Genetic mapping of microsatellite markers around the arcelin bruchid resistance locus in common bean

Publication Overview
TitleGenetic mapping of microsatellite markers around the arcelin bruchid resistance locus in common bean
AuthorsBlair MW, Muñoz C, Buendía HF, Flower J, Bueno JM, Cardona C
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume121
Issue2
Year2010
Page(s)393-402
CitationBlair MW, Muñoz C, Buendía HF, Flower J, Bueno JM, Cardona C. Genetic mapping of microsatellite markers around the arcelin bruchid resistance locus in common bean. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2010 Jul; 121(2):393-402.

Abstract

The deployment in common beans (Phaseolus vulgaris L.) of arcelin-based bruchid resistance could help reduce post-harvest storage losses to the Mexican bean weevil [(Zabrotes subfasciatus (Boheman)]. Arcelin is a member of the arcelin-phytohemagglutinin-alpha-amylase inhibitor (APA) family of seed proteins, which has been extensively studied but not widely used in bean breeding programs. The purpose of this study was to evaluate microsatellite markers for genetic analysis of arcelin-based bruchid resistance and to determine the orientation of markers and the rate of recombination around the APA locus. A total of 10 previously developed microsatellites and 22 newly developed markers based on a sequenced BAC from the APA locus were screened for polymorphism and of these 15 were mapped with an F(2) population of 157 individuals resulting from a susceptible x resistant cross of SEQ1006 x RAZ106 that segregated for both the arcelin 1 allele and resistance to the bruchid, Z. subfasciatus. Microsatellites derived from APA gene sequences were linked within 0.8 cM of each other and were placed relative to the rest of the b04 linkage group. In a comparison of genetic to physical distance on the BAC sequence, recombination was found to be moderate with a ratio of 125 kb/cM, but repressed within the APA locus itself. Several markers were predicted to be very effective for genetic studies or marker-assisted selection, based on their significant associations with bruchid resistance and on low adult insect emergence and positions flanking the arcelin and phytohemagglutinin genes.

Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
SEQ1006SEQ1006breeding_research_material
RAZ106RAZ106breeding_research_material
SEQ1006/RAZ106-F2:3SEQ1006/RAZ106-F2:3population
Featuremaps
This publication contains information about 1 maps:
Map Name
Bean-SEQ1006/RAZ106-F2:3
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2010 Jul
Journal AbbreviationTheor. Appl. Genet.
DOI10.1007/s00122-010-1318-5
Elocation10.1007/s00122-010-1318-5
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany
Publication TypeResearch Support, Non-U.S. Gov't
Cross References
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DatabaseAccession
PMID: PubMedPMID:20358173