Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.)

Publication Overview
TitleSequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.)
AuthorsHuttel B, Winter P, Weising K, Choumane W, Weigand F, Kahl G
TypeJournal Article
Journal NameGenome
Volume42
Year1999
Page(s)210-217
CitationHuttel B, Winter P, Weising K, Choumane W, Weigand F, Kahl G. Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.). Genome. 1999; 42:210-217.

Abstract

Two small-insert genomic libraries of chickpea (Cicer arietinum L.) were screened with a set of microsatellite-specific oligonucleotide probes. A total of 121 positive clones were identified among 13,000 plated colonies. Thirty-nine clones were recognized by (TAA)5, 26 by (GA)8, 18 by (GT)8, 27 by a pool of AT-rich trinucleotide repeats [(CAA)5, (CAT)5, and (GAA)5], and 11 by a pool of GC-rich trinucleotides [(TCC)5, (CAC)5, (CAG)5, and (CGA)5]. Of 53 clones selected for sequencing, 43 carried a microsatellite. Flanking primer pairs were designed for 28 loci, and used on a small test-set comprising one C. reticulatum and four C. arietinum accessions. Separation of the PCR products on agarose or polyacrylamide gels revealed single bands of the expected size with 22 of the primer pairs. Sixteen of these "Cicer arietinum sequence-tagged microsatellite site" (CaSTMS) markers were polymorphic at an intraspecific level, detecting 2-4 alleles within the four accessions examined. Primer pairs CaSTMS10 and CaSTMS15 revealed 25 and 16 alleles among 63 C. arietinum accessions from different geographic locations, reflecting gene diversity values of 0.937 and 0.922, respectively. Mendelian inheritance of CaSTMS markers was demonstrated using a set of recombinant inbred lines and their parents.
Features
This publication contains information about 22 features:
Feature NameUniquenameType
CaSTMS10CaSTMS10genetic_marker
CaSTMS11CaSTMS11genetic_marker
CaSTMS12CaSTMS12genetic_marker
CaSTMS13CaSTMS13genetic_marker
CaSTMS14CaSTMS14genetic_marker
CaSTMS15CaSTMS15genetic_marker
CaSTMS16CaSTMS16genetic_marker
CaSTMS19CaSTMS19genetic_marker
CaSTMS2CaSTMS2genetic_marker
CaSTMS20CaSTMS20genetic_marker
CaSTMS21CaSTMS21genetic_marker
CaSTMS22CaSTMS22genetic_marker
CaSTMS23CaSTMS23genetic_marker
CaSTMS24CaSTMS24genetic_marker
CaSTMS25CaSTMS25genetic_marker
CaSTMS28CaSTMS28genetic_marker
CaSTMS4CaSTMS4genetic_marker
CaSTMS5CaSTMS5genetic_marker
CaSTMS6CaSTMS6genetic_marker
CaSTMS7CaSTMS7genetic_marker
CaSTMS8CaSTMS8genetic_marker
CaSTMS9CaSTMS9genetic_marker
Properties
Additional details for this publication include:
Property NameValue
URLhttp://www.ncbi.nlm.nih.gov/pubmed/10231957