Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers

Publication Overview
TitleConstruction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers
AuthorsGupta M, Verma B, Kumar N, Chahota RK, Rathour R, Sharma SK, Bhatia S, Sharma TR
TypeJournal Article
Journal NameJournal of genetics
Volume91
Issue3
Year2012
Page(s)279-87
CitationGupta M, Verma B, Kumar N, Chahota RK, Rathour R, Sharma SK, Bhatia S, Sharma TR. Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers. Journal of genetics. 2012 Dec; 91(3):279-87.

Abstract

Lentil (Lens culinaris ssp. culinaris), is a self-pollinating diploid (2n = 2x = 14), cool-season legume crop and is consumed worldwide as a rich source of protein (~24.0%), largely in vegetarian diets. Here we report development of a genetic linkage map of Lens using 114 F(2) plants derived from the intersubspecific cross between L 830 and ILWL 77. RAPD (random amplified polymorphic DNA) primers revealed more polymorphism than ISSR (intersimple sequence repeat) and SSR (simple sequence repeat) markers. The highest proportion (30.72%) of segregation distortion was observed in RAPD markers. Of the 235 markers (34 SSR, 9 ISSR and 192 RAPD) used in the mapping study, 199 (28 SSRs, 9 ISSRs and 162 RAPDs) were mapped into 11 linkage groups (LGs), varying between 17.3 and 433.8 cM and covering 3843.4 cM, with an average marker spacing of 19.3 cM. Linkage analysis revealed nine major groups with 15 or more markers each and two small LGs with two markers each, and 36 unlinked markers. The study reported assigning of 11 new SSRs on the linkage map. Of the 66 markers with aberrant segregation, 14 were unlinked and the remaining 52 were mapped. ISSR and RAPD markers were found to be useful in map construction and saturation. The current map represents maximum coverage of lentil genome and could be used for identification of QTL regions linked to agronomic traits, and for marker-assisted selection in lentil.

Features
This publication contains information about 83 features:
Feature NameUniquenameType
ISSR1ISSR1genetic_marker
ISSR11ISSR11genetic_marker
ISSR12ISSR12genetic_marker
ISSR14ISSR14genetic_marker
ISSR21ISSR21genetic_marker
ISSR23ISSR23genetic_marker
OPA09OPA09genetic_marker
OPA13OPA13genetic_marker
OPA16OPA16genetic_marker
OPAB04OPAB04genetic_marker
OPAB11OPAB11genetic_marker
OPAB18OPAB18genetic_marker
OPAB19OPAB19genetic_marker
OPAE03OPAE03genetic_marker
OPAE04OPAE04genetic_marker
OPAE07OPAE07genetic_marker
OPAE13OPAE13genetic_marker
OPAE19OPAE19genetic_marker
OPAE20OPAE20genetic_marker
OPAI07OPAI07genetic_marker
OPAI08OPAI08genetic_marker
OPAI11OPAI11genetic_marker
OPAI12OPAI12genetic_marker
OPAI16OPAI16genetic_marker
OPAI18OPAI18genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
lentil-L830xILWL77-F2
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN0973-7731
eISSN0973-7731
Publication Date2012 Dec
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal AbbreviationJ. Genet.
Journal CountryIndia
Publication TypeResearch Support, Non-U.S. Gov't
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:23271013
AGL: USDA National Agricultural LibraryAGL:4941592