Characterization and genetic analysis of an EIN4-like sequence (CaETR-1) located in QTL(AR1) implicated in ascochyta blight resistance in chickpea

Publication Overview
TitleCharacterization and genetic analysis of an EIN4-like sequence (CaETR-1) located in QTL(AR1) implicated in ascochyta blight resistance in chickpea
AuthorsMadrid E, Rajesh PN, Rubio J, Gil J, Millán T, Chen W
TypeJournal Article
Journal NamePlant cell reports
Volume31
Issue6
Year2012
Page(s)1033-42
CitationMadrid E, Rajesh PN, Rubio J, Gil J, Millán T, Chen W. Characterization and genetic analysis of an EIN4-like sequence (CaETR-1) located in QTL(AR1) implicated in ascochyta blight resistance in chickpea. Plant cell reports. 2012 Jun; 31(6):1033-42.

Abstract

Two alleles of a chickpea (Cicer arietinum L.) ethylene receptor-like sequence (CaETR-1) were sequence-characterized using synteny analysis with genome sequences of Medicago truncatula L. The full length of the sequence obtained in the accession FLIP84-92C resistant to ascochyta blight (CaETR-1a) span 4,428 bp, including the polyadenylation signal in the 3'-untranslated region (UTR), whereas it has a 730 bp deletion in the 3'-UTR region in the susceptible accession PI359075 (CaETR-1b). The deduced protein belongs to subfamily II of the ethylene receptors and contains all the domains that define EIN4 homologs in Arabidopsis. The EIN4-like sequence (CaETR-1) has been mapped using a recombinant inbred line (RIL) population derived from an intraspecific cross between ILC3279 and WR315, resistant and susceptible to blight, respectively. The locus was located in LGIVa of the genetic map, flanked by markers NCPGR91 and GAA47 (at distances of 11.3 and 17.9 cM, respectively). This is the first potentially functional sequence identified under a QTL peak for ascochyta blight resistance in chickpea (QTL(AR1)). This EIN4-like (CaETR-1) sequence explained up to 33.8% of the total phenotypic variation. This sequence could be directly related to blight resistance, together with other QTLs that have been found to be involved in resistance to this major chickpea disease.

Features
This publication contains information about 4 features:
Feature NameUniquenameType
Ascochyta blight resistanceqABR.WR315xILC3279.LGIVa.2002QTL
Ascochyta blight resistanceqABR.WR315xILC3279.LGIVa.2003QTL
HQ009511HQ009511.1region
HQ009510HQ009510.1region
Projects
This publication contains information about 1 projects:
Project NameDescription
Chickpea-ascochyta_blight_resistance-Madrid-2013
Properties
Additional details for this publication include:
Property NameValue
Elocation10.1007/s00299-011-1221-9
Publication ModelPrint-Electronic
ISSN1432-203X
eISSN1432-203X
Publication Date2012 Jun
Journal AbbreviationPlant Cell Rep.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
DOI10.1007/s00299-011-1221-9
Journal CountryGermany
Publication TypeResearch Support, Non-U.S. Gov't