LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2

Overview
Library NameLIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
Unique NameLIBEST_026413
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
  • Cultivar: ICC4958
  • Tissue: Shoot
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
HO067090HO067090EST
HO067151HO067151EST
HO066418HO066418EST
HO066421HO066421EST
HO066447HO066447EST
HO066515HO066515EST
HO066546HO066546EST
HO066609HO066609EST
HO066623HO066623EST
HO066652HO066652EST
HO066700HO066700EST
HO066726HO066726EST
HO066807HO066807EST
HO067017HO067017EST
HO066279HO066279EST
HO066293HO066293EST
HO066419HO066419EST
HO066420HO066420EST
HO066514HO066514EST
HO066701HO066701EST
HO066859HO066859EST
HO067070HO067070EST
HO066445HO066445EST
HO066562HO066562EST
HO066650HO066650EST

Pages