LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2

Overview
Library NameLIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
Unique NameLIBEST_026413
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
  • Cultivar: ICC4958
  • Tissue: Shoot
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO067184HO067184EST
HO066659HO066659EST
HO066728HO066728EST
HO067033HO067033EST
HO067007HO067007EST
HO066655HO066655EST
HO066357HO066357EST
HO067072HO067072EST
HO067068HO067068EST
HO066682HO066682EST
HO066731HO066731EST
HO066291HO066291EST
HO066762HO066762EST
HO066949HO066949EST
HO066929HO066929EST
HO067130HO067130EST
HO066381HO066381EST
HO067208HO067208EST
HO066603HO066603EST
HO066884HO066884EST
HO067031HO067031EST
HO066683HO066683EST
HO066615HO066615EST
HO066781HO066781EST
HO067143HO067143EST

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