LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1

Overview
Library NameLIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
Unique NameLIBEST_026412
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
  • Cultivar: ICC4958
  • Tissue: Shoot
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stress plants as tester and that from the control plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO065905HO065905EST
HO065877HO065877EST
HO065853HO065853EST
HO064876HO064876EST
HO065441HO065441EST
HO065848HO065848EST
HO065834HO065834EST
HO066244HO066244EST
HO065521HO065521EST
HO066037HO066037EST
HO065768HO065768EST
HO065782HO065782EST
HO065433HO065433EST
HO065756HO065756EST
HO065120HO065120EST
HO065753HO065753EST
HO064910HO064910EST
HO065741HO065741EST
HO065749HO065749EST
HO066149HO066149EST
HO065619HO065619EST
HO065586HO065586EST
HO066095HO066095EST
HO066140HO066140EST
HO066233HO066233EST

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