LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1

Overview
Library NameLIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
Unique NameLIBEST_026412
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
  • Cultivar: ICC4958
  • Tissue: Shoot
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stress plants as tester and that from the control plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO065415HO065415EST
HO065079HO065079EST
HO065960HO065960EST
HO065026HO065026EST
HO065913HO065913EST
HO065455HO065455EST
HO065816HO065816EST
HO065899HO065899EST
HO066142HO066142EST
HO065491HO065491EST
HO065203HO065203EST
HO065180HO065180EST
HO065224HO065224EST
HO065328HO065328EST
HO065466HO065466EST
HO065626HO065626EST
HO065692HO065692EST
HO065712HO065712EST
HO065173HO065173EST
HO065746HO065746EST
HO065751HO065751EST
HO065823HO065823EST
HO065860HO065860EST
HO066196HO066196EST
HO064950HO064950EST

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