LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1

Overview
Library NameLIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
Unique NameLIBEST_026412
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
  • Cultivar: ICC4958
  • Tissue: Shoot
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stress plants as tester and that from the control plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO065600HO065600EST
HO065503HO065503EST
HO065142HO065142EST
HO065560HO065560EST
HO066163HO066163EST
HO065553HO065553EST
HO066259HO066259EST
HO065526HO065526EST
HO065520HO065520EST
HO065504HO065504EST
HO065185HO065185EST
HO065797HO065797EST
HO065949HO065949EST
HO065500HO065500EST
HO065997HO065997EST
HO065200HO065200EST
HO065467HO065467EST
HO065708HO065708EST
HO065602HO065602EST
HO064847HO064847EST
HO065947HO065947EST
HO065487HO065487EST
HO065577HO065577EST
HO065486HO065486EST
HO065442HO065442EST

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