LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2

Overview
Library NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
Unique NameLIBEST_026411
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
  • Cultivar: ICC4958
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO064574HO064574EST
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HO064348HO064348EST
HO064362HO064362EST
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HO064604HO064604EST
HO064591HO064591EST
HO064796HO064796EST
HO064588HO064588EST
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HO064587HO064587EST
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HO064561HO064561EST
HO064548HO064548EST
HO064541HO064541EST
HO064525HO064525EST
HO064372HO064372EST
HO064523HO064523EST
HO064522HO064522EST
HO064506HO064506EST
HO064632HO064632EST
HO064626HO064626EST

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