LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2

Overview
Library NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
Unique NameLIBEST_026411
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
  • Cultivar: ICC4958
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO064190HO064190EST
HO064309HO064309EST
HO064316HO064316EST
HO064370HO064370EST
HO064406HO064406EST
HO064460HO064460EST
HO064536HO064536EST
HO064617HO064617EST
HO064648HO064648EST
HO064723HO064723EST
HO064749HO064749EST
HO064756HO064756EST
HO064833HO064833EST
HO064034HO064034EST
HO064041HO064041EST
HO064078HO064078EST
HO064092HO064092EST
HO064104HO064104EST
HO064117HO064117EST
HO064243HO064243EST
HO064250HO064250EST
HO064269HO064269EST
HO064294HO064294EST
HO064306HO064306EST
HO064355HO064355EST

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