LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2

Overview
Library NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
Unique NameLIBEST_026411
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
  • Cultivar: ICC4958
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO064268HO064268EST
HO064277HO064277EST
HO064321HO064321EST
HO064332HO064332EST
HO064390HO064390EST
HO064422HO064422EST
HO064444HO064444EST
HO064563HO064563EST
HO064619HO064619EST
HO064036HO064036EST
HO064069HO064069EST
HO064087HO064087EST
HO064090HO064090EST
HO064128HO064128EST
HO064159HO064159EST
HO064331HO064331EST
HO064357HO064357EST
HO064414HO064414EST
HO064432HO064432EST
HO064450HO064450EST
HO064483HO064483EST
HO064502HO064502EST
HO064560HO064560EST
HO064681HO064681EST
HO064717HO064717EST

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