LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2

Overview
Library NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
Unique NameLIBEST_026411
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
  • Cultivar: ICC4958
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
HO064103HO064103EST
HO064169HO064169EST
HO064176HO064176EST
HO064198HO064198EST
HO064208HO064208EST
HO064222HO064222EST
HO064235HO064235EST
HO064280HO064280EST
HO064327HO064327EST
HO064345HO064345EST
HO064352HO064352EST
HO064378HO064378EST
HO064437HO064437EST
HO064442HO064442EST
HO064455HO064455EST
HO064514HO064514EST
HO064558HO064558EST
HO064565HO064565EST
HO064572HO064572EST
HO064684HO064684EST
HO064697HO064697EST
HO064741HO064741EST
HO064802HO064802EST
HO064815HO064815EST
HO064828HO064828EST

Pages