LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2

Overview
Library NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
Unique NameLIBEST_026415
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
  • Cultivar: RILs derived from cross between ICC4958 and ICC1882
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: A RIL population was derived from cross between ICC4958 a high root biomass genotype and ICC1882 a low root biomass genotype. 10 extreme RILs exhibiting HRB and LRB were selected. Bulks of the 10 RILs were used for construction of cDNA SSH library. RILs were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and equal amount of total RNA from 10 HRB RIL and 10 LRB were mixed to produce two Bulks of RNA, further mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from water stressed bulk of 10 LRB RILs plants as tester and that from the bulk of 10 RILs with HRB plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO067931HO067931EST
HO067930HO067930EST
HO067929HO067929EST
HO067928HO067928EST
HO067932HO067932EST
HO067665HO067665EST
HO067666HO067666EST
HO067891HO067891EST
HO068014HO068014EST
HO067954HO067954EST
HO067816HO067816EST
HO067909HO067909EST
HO067962HO067962EST
HO067798HO067798EST
HO067912HO067912EST
HO067851HO067851EST
HO067683HO067683EST
HO067827HO067827EST
HO067800HO067800EST
HO067862HO067862EST
HO067681HO067681EST
HO067795HO067795EST
HO067902HO067902EST
HO067656HO067656EST
HO067759HO067759EST

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