LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2

Overview
Library NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
Unique NameLIBEST_026415
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
  • Cultivar: RILs derived from cross between ICC4958 and ICC1882
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: A RIL population was derived from cross between ICC4958 a high root biomass genotype and ICC1882 a low root biomass genotype. 10 extreme RILs exhibiting HRB and LRB were selected. Bulks of the 10 RILs were used for construction of cDNA SSH library. RILs were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and equal amount of total RNA from 10 HRB RIL and 10 LRB were mixed to produce two Bulks of RNA, further mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from water stressed bulk of 10 LRB RILs plants as tester and that from the bulk of 10 RILs with HRB plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO067907HO067907EST
HO067904HO067904EST
HO067845HO067845EST
HO067840HO067840EST
HO067839HO067839EST
HO067826HO067826EST
HO067805HO067805EST
HO067799HO067799EST
HO067791HO067791EST
HO067790HO067790EST
HO067788HO067788EST
HO067784HO067784EST
HO067776HO067776EST
HO067772HO067772EST
HO067769HO067769EST
HO067757HO067757EST
HO067750HO067750EST
HO067747HO067747EST
HO067745HO067745EST
HO067729HO067729EST
HO067893HO067893EST
HO067717HO067717EST
HO067716HO067716EST
HO067714HO067714EST
HO067701HO067701EST

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