LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2

Overview
Library NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
Unique NameLIBEST_026415
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
  • Cultivar: RILs derived from cross between ICC4958 and ICC1882
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: A RIL population was derived from cross between ICC4958 a high root biomass genotype and ICC1882 a low root biomass genotype. 10 extreme RILs exhibiting HRB and LRB were selected. Bulks of the 10 RILs were used for construction of cDNA SSH library. RILs were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and equal amount of total RNA from 10 HRB RIL and 10 LRB were mixed to produce two Bulks of RNA, further mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from water stressed bulk of 10 LRB RILs plants as tester and that from the bulk of 10 RILs with HRB plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO067739HO067739EST
HO067819HO067819EST
HO067856HO067856EST
HO067855HO067855EST
HO067857HO067857EST
HO067737HO067737EST
HO067852HO067852EST
HO067848HO067848EST
HO067679HO067679EST
HO068035HO068035EST
HO067874HO067874EST
HO067640HO067640EST
HO067639HO067639EST
HO067632HO067632EST
HO067634HO067634EST
HO067636HO067636EST
HO067633HO067633EST
HO067637HO067637EST
HO067635HO067635EST
HO067638HO067638EST
HO067873HO067873EST
HO067696HO067696EST
HO067875HO067875EST
HO067735HO067735EST
HO067859HO067859EST

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