PRIMER_SEQUENCE_ID=SSR_00000 Ps_contig_mira-and-tgicl-ass_franssen_26
SEQUENCE=TCTTCTTCGTAGATTTACCTTTKGKATTTTTTTTTTCCTTCCAGTTTCATTATCACCTTCAGAACCTGAATGTAAAGAAGACGACTCATCATTAGATGAGTCATCACCCTCATCATCATCACTCCACGCCCACCAAACTTCTTGGGCTCACAGCGTCTAGGATCAGCAACAAGAAGAGTACGATCATATCGAACAAGAATATCCTTGATCTCCTTCTTACTCTGTTCATCGACATACTTCTGATAGAAAGCCACCAACGCCTTCGCGA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00001 Ps_contig_mira-and-tgicl-ass_franssen_38
SEQUENCE=AAGCAGTGGTACAACGCAGRGTTACCTTTCTTTTCTTCAACCATAATATCTCAACAACTCTATCCCCCATTCGATTCCTACACTTACTTACTCTCTAATTTTGTTACGAAATATAATTGGTTGCAATTAACATCACTTTGTGATAGCAAAAATGACAGATCAAGAGCAACCAAAAAAGGTAGAACCTGAATCAAGTTCTAATCCTTTATCAGCACCACCACCAAAGGAAGATGTGGCTCAGGAGAAATCTGTAATTCCACAACCTGATCATCTAGATCACAACCCTCCTCCTCCTCCGCCTGTTGTTGATGACTCCAAAGCTCTGGTTGTAGTTCCGAAGACCGAAGAAGCTGCTGAAGAGAAACCACAAGAGGGCTCTATCAACAGAGATGCGGTGCTTGAAAGAGTTGCAACAGAAAAGAGGCTGTCACTGGTCAAAGCATGGGAAGAAAGTGAAAAGTCTAAAGCAGAAAACAAAGCTCAGAGAAAACTGTCTACCATTACAGCATGGGAGAACAGTAAAGAAAGCTGACAACAGAAGCTGAATTAGG
TARGET=284,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00002 Ps_contig_mira-and-tgicl-ass_franssen_99
SEQUENCE=GAATCAGTTTGTCCAAAGAAGGCTTGTTTTCCAAGCAAATAGTCCACCTTAGATAGGAACTCTTCAGGTGAACCATAGTCAGTGATGGACTTCTTATCAGTTGTTTGGACTAAAACCGAAACATTGCTTGTTGCATCAAATTATCCTCATATCTAAGAACCTGACCAGGGAACTCTCTCTCTTTGCTTGGATTCCATTTTGCTGGCACCAGCAGCTTGAATCCATCTCCATTGTATGGCAAGTAATCTGTGTTTGTCTTTGCCTTTCCAAACACATTAGCAGCTTCTCCATAAGCAGCATCTGCAGGTGAAACCTTGGAGCCAACAGCAGCAGCACCAATGAGAACAGAAGTGCTAACCTACGAGACACAACAGCATCAACAACATCATTCTTTGTTTCTGTGCCTTGACAAACAATTRGTTTGGGGTTTTTGGGGTTTTTAGGGTACGGGTTAAAMCTTACGTTTGGGTCGGTTAGGAAGTAAGTTTAAACGGGTTTTCCGTTAAGGGTTTGGGGTAAGGTTTTAGTAAA
TARGET=173,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00003 Ps_contig_mira-and-tgicl-ass_franssen_104
SEQUENCE=AACACATTAGCAGCTTCTCCATAAGTTCAGCTATCTGCAGGTGAAACCTTGGAGCCAACAGCAGCAGCACCAATGAGARCAGAAAGTGCTAACCTACGAGACACAACAGCATCAACAACATCATCTTAGTTTCTGTGCCTTGACAAACAATATTGGTTTGGTTTGGTGGTAACTACTTGGCGTTGAGATAAGGTTCTAGTTGGASTTAGTAATAGCGTATTGGGTGGTGCAAAGAAACATTGTGTAGATGCCTATTTTTTACTTAAAGAGTATGCTATTTAAG
TARGET=153,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00004 Ps_contig_mira-and-tgicl-ass_franssen_123
SEQUENCE=GACAACAACACACAACANGGGTTCTTCATTTTGGACTGTGTGTTTGATTCTAGCATCATTTCTCTCTCTCTGAATTCTGTGCTCTCCCAAGGAAGCCAGTGGATGTACCATTTGGCAGAAACTATTACCCTACTTGGGCTTTTGATCACATCAAATACTTCAATGRCGGTTCTGAGATTCAGCTTCATCTTGATAAATCCACTGGTACTGGTTTTCAGTMCAAAGGTTCATACTTGTTTGGTCATTTCAGTATGAACATTAAGATGGTTCCTGGTGATTCTGCTGGAACAGTTACTGCTTTCTATTTATCTTCACAAAATGCT
TARGET=61,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00005 Ps_contig_mira-and-tgicl-ass_franssen_187
SEQUENCE=GTTAAGTTCTTTGCTGTGTCATGATTTGAAGAACACAGATCTCAAAGTTAGGTTTTATGGGCATTCAGGGTACTTGTTTTTCCCTTTTATTTGAAGGAATTTGTAGTCAGAGTATTAAGGATGCCTATATGCAGTTACAGAAAGTATTCGTTTTATATGGTTTTATGATACCAGAAGACATGCTAATGCTGGTAATGAACTTCATTGTTGATATAGTTTTAGTATTTATTGCAATTTTAGTTCTTGTNTTGCCATTGGACTATCATGATCTCGAAGGGACAAGGTATGTATTTTACCAACCAACCAACCTAAACCCTTTTGTTTAATTTTTTTCCTTACCCTTAACGTTTAAGTAAATTATTTTACCTTAAAAAGTTTAAAAAAAAAAAGT
TARGET=295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00006 Ps_contig_mira-and-tgicl-ass_franssen_205
SEQUENCE=AAGGCGCAAAGATTTAGACTTTTGAAGACCACCCTCCATATTTGATYCTTTCTTCAACCTATCATCATCATCACCTTAGTCTTCTCCACTTCTCCACCGGACAGTCTCGCCGCCGGAGCCCTTCCCCTTTCTCCGTCTCCTACGACGAATCACCGTTCCCCGATTCAGACTCCCGGCGGCGGGAGCGTGTCCAGATCTATACGAAACCACAGGCCGTAACGAACTACGAGGCGGTCGTTTGGGTCCTTTACGTAAGGAAAAAGTATTAACGTAAGGTTTAAGTAGGAAAAGGTAAGGAAAAAGCG
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00007 Ps_contig_mira-and-tgicl-ass_franssen_219
SEQUENCE=TATTCGTGTATGGGTGAAGAAAGGGGGAAGTGATCATTTTACCTGCTGGAATTTATCACCGTTTTACATTGGATGAGGGCAATTATATTAAGGCTTTGCGATTTTTTGTTGGTGAACCAGTTTGGACCCCATACAATCGCCCACACGACCATCTCCCTGCAAGAGGGGAATATATCAAGAATTTTGTGGAGAAGGATGTTGCCAACCATACTGTTGACGCCACTGCATAATTTGATTTCTGCTTGATTGTAGCATGGGATAAAAAGGACCGAGGCTTTGTTTGATACTGAAATGGTGTTTGAAAATAATAATAATGGTCTCTTTTGTTTGGTATGTAACCTTTGAGTGTTCGTGTTTGTTACCTGTGTTGTACTAGGATAATTAAAAGTTGCATTTATAAGTATTATTATTATTATTATTATTATTATTATTCAGTATTGGTGACAGAATAAACAGACAGTAATAGGATGTTGATGTATTATATATAATGTTGTACTGTTTTGGTTGCWTTYWACKTAAACT
TARGET=304,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00008 Ps_contig_mira-and-tgicl-ass_franssen_219
SEQUENCE=TATTCGTGTATGGGTGAAGAAAGGGGGAAGTGATCATTTTACCTGCTGGAATTTATCACCGTTTTACATTGGATGAGGGCAATTATATTAAGGCTTTGCGATTTTTTGTTGGTGAACCAGTTTGGACCCCATACAATCGCCCACACGACCATCTCCCTGCAAGAGGGGAATATATCAAGAATTTTGTGGAGAAGGATGTTGCCAACCATACTGTTGACGCCACTGCATAATTTGATTTCTGCTTGATTGTAGCATGGGATAAAAAGGACCGAGGCTTTGTTTGATACTGAAATGGTGTTTGAAAATAATAATAATGGTCTCTTTTGTTTGGTATGTAACCTTTGAGTGTTCGTGTTTGTTACCTGTGTTGTACTAGGATAATTAAAAGTTGCATTTATAAGTATTATTATTATTATTATTATTATTATTATTCAGTATTGGTGACAGAATAAACAGACAGTAATAGGATGTTGATGTATTATATATAATGTTGTACTGTTTTGGTTGCWTTYWACKTAAACT
TARGET=402,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00009 Ps_contig_mira-and-tgicl-ass_franssen_304
SEQUENCE=GTTCAGAAATTCATAGATGAACTTGTTAAAATTTGATTTTCCTGGATCAAAAGCAGTATCTGAAGCATCTTCAAAATTTGGAACGGCCTTAGTTTCAGGTCCAATTTTCTTTGTTTTTGAGAAAGTATCAACTTTCATAGTCACAGAAGAAAAAACTGAAGAAGTGGCTAAAACAGAAGAGGAAACAAGCGGTGTAAAAGATAGGGAAATTGTGGTTGAAGAAAGGAAAAAAAGAAGAAACAAGTGAATCTGCAGCTTGTAGAAGGCTGAAGAAAAAGCTAAGGCTGCAAATGACTCTCTGGCTCCGCCGCCGCCGGAGAAAGAGGAAGAGAAACCGGTTGAAACAACTCCGGTGGAAAAAGTGGAACCTCCAAAGGCTTGAAGATGAGACTGTTTTGGAATTGGAGCTATTTGGAGTGAAAATAATTTATATGTAATTATGACTTGTTGTATGTAGGATTGTAATTTGTTATTTGAATGTAATATGAATGATGGTNTAG
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0822
PRIMER_LEFT_PENALTY=0.048131
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=AAGCTAAGGCTGCAAATGACTC
PRIMER_RIGHT_SEQUENCE=CACTCCAAATAGCTCCAATTCC
PRIMER_LEFT=275,22
PRIMER_RIGHT=418,22
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.0822
PRIMER_LEFT_1_PENALTY=0.048131
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=AAGCTAAGGCTGCAAATGACTC
PRIMER_RIGHT_1_SEQUENCE=ACTCCAAATAGCTCCAATTCCA
PRIMER_LEFT_1=275,22
PRIMER_RIGHT_1=417,22
PRIMER_LEFT_1_TM=60.048
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.0914
PRIMER_LEFT_2_PENALTY=0.072552
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=AAAACAGAAGAGGAAACAAGCG
PRIMER_RIGHT_2_SEQUENCE=GAGTTGTTTCAACCGGTTTCTC
PRIMER_LEFT_2=169,22
PRIMER_RIGHT_2=349,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.1067
PRIMER_LEFT_3_PENALTY=0.072552
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=AAAACAGAAGAGGAAACAAGCG
PRIMER_RIGHT_3_SEQUENCE=CACTCCAAATAGCTCCAATTCC
PRIMER_LEFT_3=169,22
PRIMER_RIGHT_3=418,22
PRIMER_LEFT_3_TM=59.927
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.1067
PRIMER_LEFT_4_PENALTY=0.072552
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=AAAACAGAAGAGGAAACAAGCG
PRIMER_RIGHT_4_SEQUENCE=ACTCCAAATAGCTCCAATTCCA
PRIMER_LEFT_4=169,22
PRIMER_RIGHT_4=417,22
PRIMER_LEFT_4_TM=59.927
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_00010 Ps_contig_mira-and-tgicl-ass_franssen_309
SEQUENCE=GGCGATTTCCTTTAACGAGGACACACACACGCCACACTCCTCTCAATCTTTTTCCCATTTCCGTTCTGTAGAAAAACCCTAACCATGCTTCTCAAAACCGCCGTAACCGCTTCTTCATCTCCCTCAATTTCTCTCTTCAATTCTGAAAACTCGCTTTTGGTTCCGTTTCTTCACCGAGGAAGTAAACCGGACCGATTAGCGGTTTGTGCTACGAAAGGCTCTAGCAATAACCGTGTTTTAACCGGTGTGTTGTTTGAGCCTTTTGTAGAGGTTAAAAAGGAACTTGATCTTGTTCCTGTTTCTCCACAAGAGTCTCTTGCTCGCCATAAATTTCATGGAGACTCTGAAGCTGCTATTAACGAGCAAATCAACGTGGAATATAATGTTTCGTATGTTTACCATACTATGTATGCGTATTTTGATAGAGATAATGTTGCGCTGAAAGGTCTTGCTAAGTTTTTTAAAGAATCAAGTGAAGAGGAAAGAGGGCATGCTGAGAAGTTGATGGAATATCAGAACAAAAGGGGTGGAAAAGTGAAGTTGCAATCTATAGTGACACCGCTATTGGAACATAAAATGAGAACGCTGTAAATGGTTTATTAAGTGTTAAATTTGGTTATTATATAAGGTTTATGAAGTTTAAACATTAATTTAGGACCATAAACTT
TARGET=21,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7403
PRIMER_LEFT_PENALTY=2.703497
PRIMER_RIGHT_PENALTY=0.036789
PRIMER_LEFT_SEQUENCE=GCGATTTCCTTTAACGAGGA
PRIMER_RIGHT_SEQUENCE=ACCGGTTAAAACACGGTTATTG
PRIMER_LEFT=1,20
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=59.297
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=2.8900
PRIMER_LEFT_1_PENALTY=2.703497
PRIMER_RIGHT_1_PENALTY=0.186529
PRIMER_LEFT_1_SEQUENCE=GCGATTTCCTTTAACGAGGA
PRIMER_RIGHT_1_SEQUENCE=CACGGTTATTGCTAGAGCCTTT
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=59.297
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=2.9572
PRIMER_LEFT_2_PENALTY=2.920402
PRIMER_RIGHT_2_PENALTY=0.036789
PRIMER_LEFT_2_SEQUENCE=GGCGATTTCCTTTAACGAGG
PRIMER_RIGHT_2_SEQUENCE=ACCGGTTAAAACACGGTTATTG
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=60.920
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=2.9859
PRIMER_LEFT_3_PENALTY=2.703497
PRIMER_RIGHT_3_PENALTY=0.282365
PRIMER_LEFT_3_SEQUENCE=GCGATTTCCTTTAACGAGGA
PRIMER_RIGHT_3_SEQUENCE=CGGTTAAAACACGGTTATTGCT
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=59.297
PRIMER_RIGHT_3_TM=60.282
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=3.1069
PRIMER_LEFT_4_PENALTY=2.920402
PRIMER_RIGHT_4_PENALTY=0.186529
PRIMER_LEFT_4_SEQUENCE=GGCGATTTCCTTTAACGAGG
PRIMER_RIGHT_4_SEQUENCE=CACGGTTATTGCTAGAGCCTTT
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=60.920
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_00011 Ps_contig_mira-and-tgicl-ass_franssen_310
SEQUENCE=ACTTTTTTTAAATTTAGTAGAGAWTCACAATACACATTCACATGCATACTAAACTAAGACAAAGTACWAAATTACTAATTACACAATATAATAAAAGAATAAGAGTATTTTGGTATGTAACATTTTTTTTTGAATATATGCAAACTATTACACATATACAACATATTTATGATCTTCAAGCCTTTGGAGGTTCCACTTTTTCCACCGGAGTTGTTTCAACCGGTTTCTCTTCCTCTTTCTCCGGCGGCGGCGGAGCCAGAGAGTCATTTGCAGCCTTAGTCTTTTTYYTCAGTCCTTCTCAGCTGCAGTATTCTACTTTTGTTTTCTTTTCCTTTTTTCCTCTTACCAACCTACTTAACTTTCCCTATTCCTTTACNCCGTTCTTTCGTTTCCYYTCTKTCTTGWYTACNCCTTACTTTCTTTCTTTAGTTTTTTCTCTGTCTTAGGAACGTACCGACG
TARGET=242,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00012 Ps_contig_mira-and-tgicl-ass_franssen_310
SEQUENCE=ACTTTTTTTAAATTTAGTAGAGAWTCACAATACACATTCACATGCATACTAAACTAAGACAAAGTACWAAATTACTAATTACACAATATAATAAAAGAATAAGAGTATTTTGGTATGTAACATTTTTTTTTGAATATATGCAAACTATTACACATATACAACATATTTATGATCTTCAAGCCTTTGGAGGTTCCACTTTTTCCACCGGAGTTGTTTCAACCGGTTTCTCTTCCTCTTTCTCCGGCGGCGGCGGAGCCAGAGAGTCATTTGCAGCCTTAGTCTTTTTYYTCAGTCCTTCTCAGCTGCAGTATTCTACTTTTGTTTTCTTTTCCTTTTTTCCTCTTACCAACCTACTTAACTTTCCCTATTCCTTTACNCCGTTCTTTCGTTTCCYYTCTKTCTTGWYTACNCCTTACTTTCTTTCTTTAGTTTTTTCTCTGTCTTAGGAACGTACCGACG
TARGET=416,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00013 Ps_contig_mira-and-tgicl-ass_franssen_312
SEQUENCE=GGTTTTTTATTGTAACATTTTTTTGTTAATATATGNAACTATATACATATACAACANATTTATGATCTTCAAGCCCTTTGGTAGGGTTCCACYTTTYYACCGGTTAGTTGTTTCAACCGGTTTCTCTTCCTCTTCTCCGGCGGCGGCGGAGCCAGAGAGTCATTTGCAGCCTTAGCTTTTCTTCAGCCTTCTCAGCTGCAGATTCACTTGTTTCTTCTTTTTTCCTTCTTCAACCACAATTTCCCTATCTTTTACACCGCTTGTTTCCTCTTCTGTTTTAGCCACTTTAACTTAACAAGTTTTTTACTTCTGTGACTATGAAAGTTNATACTTTCACAAAAACAAAGAAATTGGACCTGAAACTAAGGCCGTTACC
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00014 Ps_contig_mira-and-tgicl-ass_franssen_344
SEQUENCE=GGTTGACGGAAGCTTCCACCATAACCCGATGAGTTACTACCTAGATCGACGATGGTATTCTTGGCCAGTGACCTTCTCCATGATTGTAGGGTGCTGATCAACCATGGTGACTTTCCTTTCACGTAGGGTTTTAGCGAAAGGATGAGAGAACGCTAGAGAGAGAGACGCAGGAAGAGGTTTGTGTGGAGGCGTGTGCGTGCCTTTTC
TARGET=155,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2568
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=1.252475
PRIMER_LEFT_SEQUENCE=GTATTCTTGGCCAGTGACCTTC
PRIMER_RIGHT_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT=54,22
PRIMER_RIGHT=187,21
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.748
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=1.3828
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=1.252475
PRIMER_LEFT_1_SEQUENCE=TATTCTTGGCCAGTGACCTTCT
PRIMER_RIGHT_1_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=187,21
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.748
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=1.4258
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=1.252475
PRIMER_LEFT_2_SEQUENCE=GACCTTCTCCATGATTGTAGGG
PRIMER_RIGHT_2_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=187,21
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=59.748
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=119
PRIMER_PAIR_PENALTY_3=1.5330
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=1.528639
PRIMER_LEFT_3_SEQUENCE=GTATTCTTGGCCAGTGACCTTC
PRIMER_RIGHT_3_SEQUENCE=CCTCCACACAAACCTCTTCCT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=188,21
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.529
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=1.5925
PRIMER_LEFT_4_PENALTY=0.340007
PRIMER_RIGHT_4_PENALTY=1.252475
PRIMER_LEFT_4_SEQUENCE=AGATCGACGATGGTATTCTTGG
PRIMER_RIGHT_4_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=187,21
PRIMER_LEFT_4_TM=60.340
PRIMER_RIGHT_4_TM=59.748
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_00015 Ps_contig_mira-and-tgicl-ass_franssen_367
SEQUENCE=TTTGAGTCGTTTGGGGGTACGTTGTAAGTTTGGTCCTTTCGTCCACTTTCMTCCGTATGAACATACAACCATATATCTAATTGACCACATCTTGATTACTAAGTATTCTTCTCCCTAAAATGTACTATATAATTACCCTTCTTAATTAATTATGAGCTAAATTTCAAAATAAATAAGTAATAGATGGAAGTAATTATTCTCCATCACACATTCTCTCATATAAAGCTCCAAAACAAAATAATGTTACTTTTGTTGCATAAATAAATAAACAAGTTAATATCAAAGTGTGTAATTTATGGTGTTTCACTTTCACCACTAAATTTTTCTATGTTTGTTAGGTCAAAGATGTGGCCACGATTCTACATTGGAAGCTATAATCTCGTGGTTAAAGG
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00016 Ps_contig_mira-and-tgicl-ass_franssen_378
SEQUENCE=TTTTACGTTTTCGGGGTTTTTAAGTTTHGVGACATCAAACATTACTATATAGCATTGTCATTTGAATCTTCATTGCACCAATGTCTACCTCTTCAATAAACGGCTTTAGTCTTTCTTCTTTGTCCCCTGCCAAACTTCTACCAAAAGAACTACATTGAGACCCTTTGTTTCTGCATCTCTTAACACTTCTTCTTCATCTTCTTCCTCGACTTCCTTCTCTTATCAGACAGCCGGTTTTCGTCTTCTTCTTCTCCTATCATCA
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00017 Ps_contig_mira-and-tgicl-ass_franssen_392
SEQUENCE=TRKTGGACCTTGTAATTAGGAGCTCTATTTAATCATGGCTTAAGTATGACAAATCATTATGCAACACATTCATGAAGTAAAGGCTTAGAATATCTTGTATACGAATGTTACTCATGTTTAAAGACAATAAATATCCGCCATAGGAAAGGTTACATTCTAAACAAATACCAGAACTCAGAAACTGTTCTATCTATCTATCACCTTCAGCTGAATATTTCGTAAAATTTACTCGGTGACGAGTAAACCCGAGTCTATCGACTCCAAAGGATCCTTTCTAAGACGGTGTCTCAAGCAGTTAGGGATGACAGTAGCAATATCCTCTACACTCACTTTGTCTCTTCCCTTCAAAGCAGCCAAAGCTTTAGCAGCTCTATTTGATACAATGTCTCCTCTCAATCCGTCCACGTTCAACTCTGCGTTGATACCACTGCTT
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00018 Ps_contig_mira-and-tgicl-ass_franssen_394
SEQUENCE=TGTAACGTTGGTACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTGCTTTGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCATCCCTAACTGCTTGAGACCACCGTCTTAAGAAAGGATTCCTTTGGAGTCGATAGACTTCGGGTTTACTCGTCSAACCGAGTAAATTTTACGAATATTCAGCTGAAGGTGATAGATAGATAGAM
TARGET=220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00019 Ps_contig_mira-and-tgicl-ass_franssen_395
SEQUENCE=TGAACGTGGACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTACTTTGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCRTCCCTAACTGCTTGAGACACCGTCTTAGAAAGGATCCTTTGGAGTCGATAGACTTCGGGGTTTTACTCGTCACCGAAGTAAATTTTACGAAATATTCAGCTGAAGGTGATAGATAGATAGAACWGTTT
TARGET=216,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00020 Ps_contig_mira-and-tgicl-ass_franssen_480
SEQUENCE=GTTCGGAGGRAAAAAATTAAACSGAGGAAGAAAGAGAGGGCTTCGACTCAACGCACTTTCTCTCTTCCTCTTCTTCTTCTTCCCACCCAAGTCATGAGCCTCTTCGGTATTGGCAGAAATCAGAGAACATTCCGACCCAAAAAAAAGTCTCCTTCTGGAAGCAAGGGAGCTCAGCTTAAGAAAACACATTGATGCAACATTAGGTAGTGGAAATCTGGAAGGGAAGCAAGTAAAGCTACCTCCGTGGAGAGGTATTTGAAT
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00021 Ps_contig_mira-and-tgicl-ass_franssen_649
SEQUENCE=TACGTCGGGGACCTTCCCTCTTCTCAACTCATCTTTTCCTTTTACTGAAAATCTGAACTAAATCTCATCACAATGGACGCAAGCAAACAAACAAAGAAAGGAGCCGGAGGAAGGAAAGGAGGAGGACCAAGGAAGAAGGCGGTCACAAGATCCGTCAGAGCCGGACTTCAATTTCCAGTTGGAAGAATCGGTCGATTCTTGAAGAAAGGTAGATACGCTCAGCGTGTTGGTACCGGTGCTCCTGTTTACTTGGCCGCTGTTCTTGAATATCTTGCTGCTGAGGTTCTTGAGTTGGCTGGAAATGCAGCACGTGATAACAAGAAGAAAAGTATCAGTCCAAGGCATTTGTTGTTGGCTGTGAGGAACGATGTAGAACTTGGAAAATTGCTTGCTGGTGTTACCATTGCTCACGGTGGTGTTCTTCCTAACATCAACCCAGTGTTATTGCCCAAGAGAAATGAAAACGCTGCCAGTACTCCCAAGTCTCCATCAAAGGCCAAGAAATCTCCAAAGAAGGCTTAGATAGATGACTTTAGATGTTAGGTTTTGTTTCTTCCTTTTTGTTGTGATGATATGTAAAACCCCTATGATGGAGCAGGGATATATTAACTTATACCAACCGATGTAATTCAATAGTCTCTGAATTTTCTGACTAGTATTATTCATCAATGAAAACGATTTGTTTTCTGTHMAC
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00022 Ps_contig_mira-and-tgicl-ass_franssen_676
SEQUENCE=CGATAGAAAACGAAGCACATGAACACCAAATGCATAAAACAAGTGAACACCAAATATAGTCAATTCCACTATTCATAGAATACACAAAAGATCCAACGGATCACATTACATCACCTTACAAGTTAAACATGAATCCTCTAAACAACGATACTGTTACAACTCTAAGCGCTAGTGAACTTGGTTACAGCTTTGGTACCCTCAGATACAGCATGCTTGGCCAATTCTCCAGGAAGAACAAGCCTAACCGCAGTCTGAATTTCCCTCGAAGTTATCGTTGGCTTCTTGTTATACCTCGCAAGCCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATATCATTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTTTCCACATTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCAGCGGCGCCACCGTCCTTGGGTAGCTTCTTTCCGGCCTTTGGCTTCTTCTCGGCGGGAGTCTTCTCGGCTATTGTGGATTTCTTCTCCTCTACAGGTTTCTTCTCAGCTGGTTTCTTCTCTCCTTTGGTGCCATTTTTGTTTGGGAAATGGAAATTGGAAATTGAAAGAAAATAGAAAGA
TARGET=452,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1735
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_SEQUENCE=AAACCAGCTGAGAAGAAACCTG
PRIMER_LEFT=345,22
PRIMER_RIGHT=586,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.1909
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_1_SEQUENCE=AAATCCACAATAGCCGAGAAGA
PRIMER_LEFT_1=345,22
PRIMER_RIGHT_1=553,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.1909
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_2_SEQUENCE=AGAAATCCACAATAGCCGAGAA
PRIMER_LEFT_2=345,22
PRIMER_RIGHT_2=555,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.1909
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_3_SEQUENCE=AAGAAATCCACAATAGCCGAGA
PRIMER_LEFT_3=345,22
PRIMER_RIGHT_3=556,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.1909
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_4_SEQUENCE=GAAATCCACAATAGCCGAGAAG
PRIMER_LEFT_4=345,22
PRIMER_RIGHT_4=554,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_00023 Ps_contig_mira-and-tgicl-ass_franssen_680
SEQUENCE=ACGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATTTTCGAGAAGCTTGCTGCTGAGTCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGAGAATTGGCCAAGCATGCCGTCTCTGAGGGTACCAAGGCTGTGACTAAGTTCACAAGCTCTTAAACTCAATATCTAGGTTTTTTCTGGGTCGCTATGTAGGCACCGTTATACCTTTCTAGTGTTGTAATCTGCTAATGGGATCGCGCTGATCCTTTTATTGTGTATGAACTTATCTTATTATTCTTAACATGATTAATGGTATTGGGCTTCAAAAATTGTTTTTATTGTAACACC
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00024 Ps_contig_mira-and-tgicl-ass_franssen_681
SEQUENCE=CCTAACAGGGAGGTACCATATCACATAATTCAAGAACTGGTGAATTTGGTGACGGCCTTTGTACCCTCGGAAACGGCGTGTTTGGCCAATTCACCAGGAAGTACCAATCTAACAGCGGTTTGAATCTCCCTTGAAGTAATAGTTGGCTTCTTGTTATACCTAGCCAAACGAGAGGATTCTTGAGCTAGTTTCTCGAAGATGTCGTTGATGAAACTATTCATGATTCCCATAGCCTTGCTGGAGACTCCGATATCAGGATGAACCTGTTTCAAAACCTTGAAGATGTAGATCTTGTAGGTCTCAACGCTCTTCTTGGATTTCTTCTTCTTCTTGTCGCTTCCTCCTTCCTTCGAGATCTTCTTCTCAGCCTTAGGTTTCTTCTCTGCAGGGGATTTCTCTGCTGCGGGTTTCTTCTCGGCTGGCTTCTTCTCGGCCTTTGTTGGAGCCATTGGTGATGTTGGTTGAATTGATGGGTTGGTCGTAGGAAGAAAAGAGAGGTTGATACAGAGATGGAGTGTTGAATCCCCGCGTACTCTGACGTTAG
TARGET=319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2040
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=0.095771
PRIMER_LEFT_SEQUENCE=GTCTCAACGCTCTTCTTGGATT
PRIMER_RIGHT_SEQUENCE=CCATCAATTCAACCAACATCAC
PRIMER_LEFT=297,22
PRIMER_RIGHT=472,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.2040
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=0.095771
PRIMER_LEFT_1_SEQUENCE=GTCTCAACGCTCTTCTTGGATT
PRIMER_RIGHT_1_SEQUENCE=CATCAATTCAACCAACATCACC
PRIMER_LEFT_1=297,22
PRIMER_RIGHT_1=471,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=175
PRIMER_PAIR_PENALTY_2=0.2136
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=0.095771
PRIMER_LEFT_2_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_2_SEQUENCE=CCATCAATTCAACCAACATCAC
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=472,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.2136
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.095771
PRIMER_LEFT_3_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_3_SEQUENCE=CATCAATTCAACCAACATCACC
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=471,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.2665
PRIMER_LEFT_4_PENALTY=0.170703
PRIMER_RIGHT_4_PENALTY=0.095771
PRIMER_LEFT_4_SEQUENCE=ATTCATGATTCCCATAGCCTTG
PRIMER_RIGHT_4_SEQUENCE=CATCAATTCAACCAACATCACC
PRIMER_LEFT_4=215,22
PRIMER_RIGHT_4=471,22
PRIMER_LEFT_4_TM=60.171
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_00025 Ps_contig_mira-and-tgicl-ass_franssen_683
SEQUENCE=AGGTTTCAAGAAAAGATTCATATTATCATTAATATTCAAAAACACATTTGTAAATCTACAATCCTTTTTATAAAATTTAAAACAAAAGACCAATTTATGATAAACTAAACCCTAATTCAAAGAAAAAGGGAAAAACAGAAAACAAATCAAGAACTGGTGAATTTTGTAACGGCCTTGGTACCCTCTGAGACGGCATGTTTAGCCAATTCACCGGGAAGAACCAATCTAACAGCGGTTTGAATTTCCCGAGAAGTAATCGTTGGCTTCTTGTTGTACCTAGCCAACCGAGACGATTCTTGAGCGAGTTTCTCAAAGATATCGTTGATGAAACTGTTCATGATTCCCATGGCCTTGCTTGAGACACCAATATCGGGGTGAACCTGTTTCAAAACCTTGAAGATGTAAATCTTGTAGGTTTCAATGCTCTTCTTGGTTCTCTTCTTCTTCTTGTCGCTTCCTCCTTCTTTTGAGATCTTCTTCTCGGCCTTTGGTTTCTTCTCGGCGGGGGATTTTTCTACGGCGGGCTTCTTCTCCGCTGGTTTCTTCTCGGCCTTTGTTGGAGCCATTGATGGTGTTGATTGGTAGGAAATTGGGTTGGTTGTGAATGCGAAAGAGATGAAGTGATGAATCCCCGABTA
TARGET=437,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00026 Ps_contig_mira-and-tgicl-ass_franssen_684
SEQUENCE=TCAACGCAGAGTAYTCGGGGACCACAATCTCAATTCTTCATCTAATTTCATCTCAACATTCCAATCTCAAACCCTATTTTCCCCAATGGCGCCAAAAGGAGAGAAGAAGCCAGCCGAGAAGAAACCCGTAGAGGAGAAGAAATCCACGGTAGCCGAGAAGGTTCCGGCTGAGAAGAAGCCAAAGGCCGGCAAGAAGCTGCCTAAAGACGGTGGTGCCACCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATCAATGATATTTTCGAGAAGCTTGCTGCGGAGTCGTCTAGACTTGCGAGGTACAACAAGAAGCCGACGATAACTTCGAGGGAAATTCAGACCGCGGTCAGGCTCGTTCTGCCCGGTGAATTGGCTAAGCATGCTGTTTCTGAGGGTACCAAAGCTGTAACTAAGTTCACTAGTTCTTAGATTTGAAACTGTTTTTAGGGGTTTTTGTATGCTTAAGTAGATCTAATGGGTCTGAATATTTTCTTTGAAACTGTGGATTTTATTTTATGACTCATAAAGTAATATGATTCAGTGAATTGACTCAAAGGTTATCTGTAAATGTTTTCTCTTTGAACAAAATCAAACAAGAAAACTAATTAAA
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00027 Ps_contig_mira-and-tgicl-ass_franssen_689
SEQUENCE=CTCCAACGAAGGCCGAGAAGAAGCCAGCCGAGAAGAAACCCGCAGCAGAGAAGTCCCCTGCAGAGAWGAAACCTAAGGCTGAGAAGAAGATCTCGAAGGAAGGAGGAAGCGACAAGAAGAAGAAGAAGTCCAAGAAGAGCGTTGAGACCTACAAGATCTACATCTTCAAGGTGTTGAAGCAGGTTCATCCTGATATCGGTGTCTCCAGCAAGGCTATGGGAATCATGAACAGTTTCATCAACGACATTTTTGAGAAACTCGCTCAGGAATCCTCCCGTTTGGCTAGGTATAACAAGAAGCCAACTATTACATCTCGGGAGATTCAAACCGCTGTTAGATTGGTTCTCCCCGGGGAGTTGGCCAAACACGCCGTCTCCGAGGGTACCAAGGCCGTCACCAAATTCACCAGTTCTTGAATTATCTGCTCTGTTATTATTCTCTCTATAGGGTTTAGCTTGATTTTTGTTGAATTAGTTTTTTTTAATGGAGCATTGATGTATATAATGGTTATAATCAATTTAAATGATATTTAAAATTCTTCACTTTWTAAT
TARGET=114,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00028 Ps_contig_mira-and-tgicl-ass_franssen_690
SEQUENCE=AAGCTCCGGTCGAGAAGAAGCCAAAGGCCGGAAAGAAGCTGCCTAAGGATGGCGGCGCCGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTTGCTGCTGAATCTTCAAGACTTGCAAGATACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACTGCAGTTAGACTTGTTCTCCCTGGAGAATTGGCCAAGCATGCTGTCTCTGAAGGAACAAAAGCCGTGACCAAGTTTACCAGTGCTTAAACTGTACCATTTGTAGTGTTGTTTCAGAAGTGGTTCTTGTTAATGTTTATCTCTCTTGGTATCAATTAAATCCGAATTATTCTGTAATTTTCTTTTAAATATTAAAATAATATCTTGTCTATAGAATAATTATATGTYYTTTGTCGTCTTGTAGGGCACAGTTTATAATGCGACA
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00029 Ps_contig_mira-and-tgicl-ass_franssen_691
SEQUENCE=CKYGGGGAACCACATCCATATTCAATTTGCAAATTCGAAAACCTAAATTCTCAATTTCTTCAATGGCGCCAAAGGGGGAGAAGAAGCCAGCTGAGAAGAAACCCGCGGAGGAGAAGAAAACGACTGTAGCTGAGAAGGCTCCGGCTGAGAAGAAGCCAAAGGCCGGAAAGAAGCTGCCTAAGGATGGCGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGCATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTAGCTGCCGAATCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCTAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGAGAATTGGCCAAGCATGCCGTCTCTGAGGGTACCAAAGCTGTGACTAAGTTCACTAGTTCTTAGATTTTTAGGTTCTTGTTTGCTGCGTTGATTTTGAACTTTTATCTTTTGTAAATTGGAATGAAGTAGTGGGAACCGTTGAATCTTTTCTGTGTATGAAAATGATGATTTTATTCTAATATACTTCAAAATGACTTATATAAAACTTGCTTTAAAATTAAAAAAGGTTT
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00030 Ps_contig_mira-and-tgicl-ass_franssen_692
SEQUENCE=GTTCGAACACCAAATATAATCAATTCCACCATTTTCAATACACAAAGGATCCAATATATCCCATTACTTCATCATACAAGCTAAAGAGTAGAAAAACTACTCTTGAAATCTATAATAACWTAAAACCAGATCCAGTAAACAAGAACCCATTAAACAACAACAAAACTGTTACAACTCTAACCGCTTGTAAACTTGGTTACAGCTTTAGTACCCTCAGACACGGCGTGTTTGGCCAATTCTCCGGGAAGAACAAGCCTAACCGCAGTCTGAATCTCCCTCGAAGTTATCGTCGGCTTCTTGTTATACCTCGCAAGCCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATGTCGTTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTCTCCACACTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCGCCACCGTCCTTGGGTAGTTTCTTTCCGGCCTTGGGTTTCTTCTCTACTGAATTTGTATC
TARGET=467,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00031 Ps_contig_mira-and-tgicl-ass_franssen_693
SEQUENCE=CTTTGGTACCCTCAGATACAGCATGCTTGGCCAATTCTCCAGGAAGAACGAGCCTAACCGCAGTCTGAATCTCCCTCGAAGTTATCGTTGGCTTCTTGTTATACCTCGCAAGTCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATATCGTTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTTTCCACATTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCNGCGGCGCCACCGTCCGTTGGGTACGTCCTTTCCGTTTCCCGGTCCTTTGGTCTTTCCTTCGTCGGGCGGGTAGTTCCTTCGTCGGTCTTATTGGTGGTTACTTTTCCTTCCTTCCCTTCCTTAACGTTACGGTTTTCCTTCCTCGTA
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0969
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.001455
PRIMER_LEFT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_SEQUENCE=AACGTTAAGGAAGGGAAGGAAG
PRIMER_LEFT=158,22
PRIMER_RIGHT=414,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.999
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1488
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.001455
PRIMER_LEFT_1_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_1_SEQUENCE=AACGTTAAGGAAGGGAAGGAAG
PRIMER_LEFT_1=183,22
PRIMER_RIGHT_1=414,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.999
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.1499
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.054491
PRIMER_LEFT_2_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_2_SEQUENCE=GGAAGGGAAGGAAGGAAAAGTA
PRIMER_LEFT_2=158,22
PRIMER_RIGHT_2=406,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.946
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.1499
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.054491
PRIMER_LEFT_3_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_3_SEQUENCE=GTTAAGGAAGGGAAGGAAGGAA
PRIMER_LEFT_3=158,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=59.946
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.1867
PRIMER_LEFT_4_PENALTY=0.185267
PRIMER_RIGHT_4_PENALTY=0.001455
PRIMER_LEFT_4_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_4_SEQUENCE=AACGTTAAGGAAGGGAAGGAAG
PRIMER_LEFT_4=195,22
PRIMER_RIGHT_4=414,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.999
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_00032 Ps_contig_mira-and-tgicl-ass_franssen_693
SEQUENCE=CTTTGGTACCCTCAGATACAGCATGCTTGGCCAATTCTCCAGGAAGAACGAGCCTAACCGCAGTCTGAATCTCCCTCGAAGTTATCGTTGGCTTCTTGTTATACCTCGCAAGTCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATATCGTTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTTTCCACATTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCNGCGGCGCCACCGTCCGTTGGGTACGTCCTTTCCGTTTCCCGGTCCTTTGGTCTTTCCTTCGTCGGGCGGGTAGTTCCTTCGTCGGTCTTATTGGTGGTTACTTTTCCTTCCTTCCCTTCCTTAACGTTACGGTTTTCCTTCCTCGTA
TARGET=391,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5560
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.460533
PRIMER_LEFT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT=158,22
PRIMER_RIGHT=433,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.539
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.6078
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.460533
PRIMER_LEFT_1_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_1_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_1=183,22
PRIMER_RIGHT_1=433,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.539
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.6458
PRIMER_LEFT_2_PENALTY=0.185267
PRIMER_RIGHT_2_PENALTY=0.460533
PRIMER_LEFT_2_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_2_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_2=195,22
PRIMER_RIGHT_2=433,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=59.539
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.8356
PRIMER_LEFT_3_PENALTY=0.375090
PRIMER_RIGHT_3_PENALTY=0.460533
PRIMER_LEFT_3_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_RIGHT_3_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_3=184,22
PRIMER_RIGHT_3=433,22
PRIMER_LEFT_3_TM=60.375
PRIMER_RIGHT_3_TM=59.539
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.9360
PRIMER_LEFT_4_PENALTY=0.475463
PRIMER_RIGHT_4_PENALTY=0.460533
PRIMER_LEFT_4_SEQUENCE=GGGTGAACTTGTTTCAGAACCT
PRIMER_RIGHT_4_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_4=200,22
PRIMER_RIGHT_4=433,22
PRIMER_LEFT_4_TM=59.525
PRIMER_RIGHT_4_TM=59.539
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_00033 Ps_contig_mira-and-tgicl-ass_franssen_694
SEQUENCE=CGGAGAAGAAACCGTGGAGGAGAAGAAGTCCACCATAGCAGAGAAGGCTCCTGCCGAGAAGAAGCCGAAGGCCGGAAAGAAGCTGCCTAAGGACGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATTTTCGAGAAGCTTAGCTGCTGAGTCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGAGAATTGGCCAAGCATGCCGTCTCTGAGGGTACCAAGGCTGTGACTAAGTTCACAGCTTACTTAAAACTCAAATTTACG
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3743
PRIMER_LEFT_PENALTY=0.226971
PRIMER_RIGHT_PENALTY=0.147295
PRIMER_LEFT_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT=25,22
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=59.773
PRIMER_RIGHT_TM=59.853
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.4122
PRIMER_LEFT_1_PENALTY=0.226971
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_1_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.4805
PRIMER_LEFT_2_PENALTY=0.226971
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_2_SEQUENCE=CTTCTCGAAAATGTCGTTGATG
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=59.773
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.5712
PRIMER_LEFT_3_PENALTY=0.423908
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=GGAGGAGAAGAAGTCCACCATA
PRIMER_RIGHT_3_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=208,22
PRIMER_LEFT_3_TM=59.576
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.6021
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.375090
PRIMER_LEFT_4_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_4_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=207,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=60.375
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_00034 Ps_contig_mira-and-tgicl-ass_franssen_695
SEQUENCE=ATGGCCTTGCTTGAGATGCCAATGTCAGGGTGAACTTGTTTCAGAACTCTGAAGATGTAGATCTTGTATGTCTCCACACTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCACCGCCATCCTTAGGCAGCTTCTTTCCGGCCTTTGGCTTCTTCTCAGCCGGAGCCTTCTCAGCTACGGTCGTTTTCTTCTCCTCCGCGGGTTTCTTCTCAGCTGGCTTCTTCTCCCCTTGGCGCCATTGAAGAAATTAG
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6097
PRIMER_LEFT_PENALTY=0.185267
PRIMER_RIGHT_PENALTY=0.424392
PRIMER_LEFT_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_SEQUENCE=GAAAACGACCGTAGCTGAGAAG
PRIMER_LEFT=22,22
PRIMER_RIGHT=194,22
PRIMER_LEFT_TM=60.185
PRIMER_RIGHT_TM=60.424
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.6097
PRIMER_LEFT_1_PENALTY=0.185267
PRIMER_RIGHT_1_PENALTY=0.424392
PRIMER_LEFT_1_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_1_SEQUENCE=GAGAAGAAAACGACCGTAGCTG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=199,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=60.424
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.6097
PRIMER_LEFT_2_PENALTY=0.185267
PRIMER_RIGHT_2_PENALTY=0.424392
PRIMER_LEFT_2_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_2_SEQUENCE=GAAGAAAACGACCGTAGCTGAG
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=60.424
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.7743
PRIMER_LEFT_3_PENALTY=0.185267
PRIMER_RIGHT_3_PENALTY=0.589035
PRIMER_LEFT_3_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_3_SEQUENCE=GAGGAGAAGAAAACGACCGTAG
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=202,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.411
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.8810
PRIMER_LEFT_4_PENALTY=0.185267
PRIMER_RIGHT_4_PENALTY=0.695721
PRIMER_LEFT_4_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_4_SEQUENCE=AAGAAGCCAGCTGAGAAGAAAC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.304
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_00035 Ps_contig_mira-and-tgicl-ass_franssen_697
SEQUENCE=AAGTCTAACCGCGGTCTGAATTTCCCTTGATGTTAATTGTTGGCTTCTTGTTGTATCCTGCAAGTCTTGAAGACTCTGCAGCAAGCTTCTCGAAGATGTCGTTGATGAAACTATTCATGATACCCATGGCCTTGCTTGAGATACCAATGTCGGGGTGAACTTGCTTCAGAACCTTGAAGATGTAGATCTTGTATGTCTCCACACTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCAGCGGCACCGCCATCCTTAGGCAGCTTCTTTCCGGCCTTTGGTTTCTTCTCGGCAGGAGCCTTCTCGGCCACCGTAGACTTCTTCTCTTCGACGGGTTTCTTCTCAGCAGGCTTCTTCTCTCCCTTTGGCGCCATTACAAGAATTGAGAAATTTAGGGTTTCGAATTGGAAATTGGATATTAAAACTGAAACGAGATC
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0424
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_RIGHT_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT=155,22
PRIMER_RIGHT=333,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.0734
PRIMER_LEFT_1_PENALTY=0.061230
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=CTATTCATGATACCCATGGCCT
PRIMER_RIGHT_1_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.1233
PRIMER_LEFT_2_PENALTY=0.030276
PRIMER_RIGHT_2_PENALTY=0.093026
PRIMER_LEFT_2_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_RIGHT_2_SEQUENCE=AGAAGAAGCCTGCTGAGAAGAA
PRIMER_LEFT_2=155,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=60.030
PRIMER_RIGHT_2_TM=59.907
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.1300
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_3_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=333,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.1368
PRIMER_LEFT_4_PENALTY=0.124655
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CTTCTCGAAGATGTCGTTGATG
PRIMER_RIGHT_4_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_00036 Ps_contig_mira-and-tgicl-ass_franssen_698
SEQUENCE=GTACGCGGGGACACCCTAATATCTGATTACGAACCCTATTTTTTCTCGAAATTTCAAATTCCCAACTCCTTCAATGGCGCCAAAGGGAGAGAAGAAGCCCGCGGAGAAGAAACCCGTAGAGGAGAAGAAGTCTACAGTAGCCGAGAAATCTCCGGCTGAGAAGAAGCCAAAGGCCGGTAAGAAGCTACCGAAGGACGGTGGTGCCGCCGCCGTTGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAGACATACAAGATCTACATCTTTAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCTATGGGAATCATGAACAGTTTCATTAACGATATCTTCGAGAAGCTCGCGGCTGAATCTTTCAGACTT
TARGET=221,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7125
PRIMER_LEFT_PENALTY=0.596759
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=GGAGAAGAAACCCGTAGAGGAG
PRIMER_RIGHT_SEQUENCE=CCCATAGCCTTGCTTGAGATAC
PRIMER_LEFT=102,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=60.597
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.7125
PRIMER_LEFT_1_PENALTY=0.596759
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=GGAGAAGAAACCCGTAGAGGAG
PRIMER_RIGHT_1_SEQUENCE=CCATAGCCTTGCTTGAGATACC
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=325,22
PRIMER_LEFT_1_TM=60.597
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.7441
PRIMER_LEFT_2_PENALTY=0.596759
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=GGAGAAGAAACCCGTAGAGGAG
PRIMER_RIGHT_2_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=60.597
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.8089
PRIMER_LEFT_3_PENALTY=0.693167
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=CAAATTCCCAACTCCTTCAATG
PRIMER_RIGHT_3_SEQUENCE=CCCATAGCCTTGCTTGAGATAC
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=326,22
PRIMER_LEFT_3_TM=60.693
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.8089
PRIMER_LEFT_4_PENALTY=0.693167
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=CAAATTCCCAACTCCTTCAATG
PRIMER_RIGHT_4_SEQUENCE=CCATAGCCTTGCTTGAGATACC
PRIMER_LEFT_4=54,22
PRIMER_RIGHT_4=325,22
PRIMER_LEFT_4_TM=60.693
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_00037 Ps_contig_mira-and-tgicl-ass_franssen_700
SEQUENCE=CTTTAATTCAAAAATTTAAGACGAAACATACCCCAYTATAAACTTATAATAACTGAAAAATACATACATAACCAAAAACAAGAGGATTGATTTGATTTTAAAAGAAAGTTACACACTAATTCAGACCTAATTCTAGCACTTATACAAAATTCAAGCACTTGTAAACTTAGTGACAGCTTTCGTCCCTTCAGAAACAGCATGCTTAGCCAATTCTCCAGGCAGAACAAGCCTGACCGCAGTCTGAATTTCCCTCGATGTGATTGTTGGCTTCTTGTTGTACCTTGCAAGTCTGGAAGATTCAGCAGCAAGCTTCTCGAAGATGTCGTTGATGAAGCTGTTCATGATTCCCATGGCCTTGCTTGAGATACCGATGTCAGGGTGAACCTGCTTCAGAACTTTGAAGATGTAGATCTTGTATGTCTCCACGCTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCACCGGCTTCCTTGGGAAGCTTCTTTCCGGCTTTGGGTTTCTTCTCGGCAGGAGACTTCTCGGCCACCGTAGACTTCTTCTCTTCAACAGGTTTCTTCTCAGCGGGCTTCTTCTCTCCCTTTGGCGCCATTGAGAATTGAGAATTTGGAACTTTGAAGAAAAATTAGGGTTTCGGATTGGATATTAGAATTGGAAATCAAATATGATAGATGATCCCGACGTACTCTGCGTTG
TARGET=441,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00038 Ps_contig_mira-and-tgicl-ass_franssen_701
SEQUENCE=AGAGAAGTCGACGAGCGNGAGACNCGCNGAGAAAAGACAAGCGGAAGAAGCNGCTAAGGATGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAGGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATCAACGACATATTCGAGAAGCTTGCTGCAGAATCTTCGAGGCTTGCAAGATACAACAAGAAGCCAACGATCACTTCCAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGTGAATTGGCGAAGCATGCCGTTTCTGAGGGCACCAAAGCCGTAACCAAGTTCACAAGCTCTTAGACTTGAATAAATTAAGCTGCCAATTATCAGTTATTTCTATTTTTAGATCTTGTTTAGGTATCCTTAGAAAGTTTAATTTAGGGTTTTTTCTAGCTTTTAAAATGGTAGGCAAAGTAATGGATTCTGTTTCTATTAAAAGTGTAGACG
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3774
PRIMER_LEFT_PENALTY=0.347129
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_LEFT=28,22
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.4070
PRIMER_LEFT_1_PENALTY=0.347129
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_1_SEQUENCE=TCTGAATTTCCCTGGAAGTGAT
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=59.653
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.4944
PRIMER_LEFT_2_PENALTY=0.347129
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_2_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=175,22
PRIMER_LEFT_2_TM=59.653
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.5524
PRIMER_LEFT_3_PENALTY=0.347129
PRIMER_RIGHT_3_PENALTY=0.205226
PRIMER_LEFT_3_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=GGTGAACTTGCTTCAGAACCTT
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=157,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=59.795
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.7222
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.375090
PRIMER_LEFT_4_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_4_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=174,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.375
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_00039 Ps_contig_mira-and-tgicl-ass_franssen_707
SEQUENCE=GKTCGGGGATTCATTCACATTTCAATCTTCCTTTTCAAAATTTCACAGTTGATAATTATAAGAAGAGAAAAATGTCAGGTCGCGGTAAAGGTGGAAAGGGTTTGGGAAAGGGAGGAGCAAAGAGGCACAGAAAAGTGCTTCGTGATAACATCCAGGGAATTACCAAGCCTGCAATTCGCCGTTTGGCGAGAAGAGGTGGTGTCAAGAGAATCAGTGGACTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATCTTTCTCGAGAACGTTATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAAACTGTCACTGCCATGGATGTTGTTTATGCTCTCAAGAGGCAGGGAAGGACTCTGTACGGTTTCGGTGGTTGATTTTATGATCCAATTTAGGGTTTCGTTAGTGTTAGTTGGGTGTAGATGTATTTTATCTATTTAAGCTGAACTGAGGCAATTTGTTATGTTCTTCTATCATATGAACTTAATGATGGATATTCTGTTTTTCTGCTAAATATATCTTTGATTTGTTATCGTTTATGATYAAAAAACNGTAATCTCAATTAATTAATTAGAAACC
TARGET=567,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00040 Ps_contig_mira-and-tgicl-ass_franssen_724
SEQUENCE=CCTCTCTACAAATTTACGTAGATTCTTCTTCTTCAACCTCATCGCATCTCTAACCAACTTTTCTCCTAGCCATCACCGTAACAAACCTTCAACCGCTTCCAATCTTCACTCTTCAATTCTTCCGCATAACTGTATGATCATCACAAYCGAACCACCAAAGATCCTGCAACAATTCTGCARCAATTCTCTCCATTCTCGGAGGTGATAAGCAGAAGGAAAAAGAAGAAATAATAGAAAAGAGAAATCCAGAAGAGATCGGAATAATCGGAAACTCACGATCCAAGAGGCTTCTTCTTCATTTCGATCGGCAATACCGGTGTAACGTCTTCGTCTTCATCTCAAAGCGGAGACGCGAGCGAGGCCTTCATCATCCACCAAGAACCTTCAACAAAATCCGTAGAAGCTCGCAACTCATAACGGAATTGATGAGAAGCTCCAATCAGAAAGAAACCGAAAATTGAAGAAGCAGAAGAAGGATAGGAGCAGAGGAGCGATTGTTCATACCGAAGCAGTGTCTAAGCGCCGTCCGCCGTATGCTCCGTCGTCGACGTCGAAGGTACGCAAAGATTCTCCTATTTGACTCGTGTTGTCGACGTTGTAAAGGCATCGATTAGGTGAGCGTTTATCCCAAATTCATGGATTTGATTACCTCCATGCGAGGACTTAATTGAAGTTTTTTACCTAGGTTTCTTAAAATGATCTTTCTGGTTCGGTCAATAAAGAAGAAGTTGGAGTAGGAAATAGGGAGTAGATTCGTGGCAGGTGGTGGAAGCGAGAATGAGTGTGGTGAGTAATTCGCCGCTGTCGGTGATGAAATTCGGCGCGGCAAGGCTTTTCCGGTGAGGAGCGTTCTTTCATTTTTCTTCTGTGAGGTTAGCGAGCGATCCCCGCGTACTCTGCGTTGAACTACCACTGCTT
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00041 Ps_contig_mira-and-tgicl-ass_franssen_728
SEQUENCE=AAATTTTTTAAAAATTCCATTTTCTCTTTTATCCTATTTATGCTAACCACATCCAATTTTAATTATCCACATTCAATTGAACCAATAGCTACCTACAAAACAACAATTCGCAACTCAAAGCTGCTACAGTTTAAGCCGAACACAACTTGAAACGCAGCCAAGGAATGAAACGTAAAGCAAGGCATCATGAGCTGCAAGCTACAACAACACCAAAACGCATAAGTCAGCTCCAAACGCACACCAGATGCTATGAACACAAGCCACCAAGCCAAACAACTTGCAAATCCATATTGAAACGCAGCTAACGCTTCCAAGCCGGTGAACCGCAAAGCAAAACGTGAGCCAACAACAAATTGCACAAATTAGCTCTCATAACTCTATAAAATCAATCTTCTTCTCATCTCCTAGATTCTTCTTCTTCAACCTCATCGCATCTCTAACCAACCTTTCTTCAGTTCTTTCAACCTTACA
TARGET=410,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3412
PRIMER_LEFT_PENALTY=0.124598
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=GAGCTGCAAGCTACAACAACAC
PRIMER_RIGHT_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT=188,22
PRIMER_RIGHT=450,22
PRIMER_LEFT_TM=60.125
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=10.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.4236
PRIMER_LEFT_1_PENALTY=0.206979
PRIMER_RIGHT_1_PENALTY=0.216588
PRIMER_LEFT_1_SEQUENCE=GCAAGCTACAACAACACCAAAA
PRIMER_RIGHT_1_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=450,22
PRIMER_LEFT_1_TM=60.207
PRIMER_RIGHT_1_TM=59.783
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=10.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.5133
PRIMER_LEFT_2_PENALTY=0.296664
PRIMER_RIGHT_2_PENALTY=0.216588
PRIMER_LEFT_2_SEQUENCE=ATATTGAAACGCAGCTAACGCT
PRIMER_RIGHT_2_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_2=287,22
PRIMER_RIGHT_2=450,22
PRIMER_LEFT_2_TM=60.297
PRIMER_RIGHT_2_TM=59.783
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=10.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.5498
PRIMER_LEFT_3_PENALTY=0.333229
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=AGGAATGAAACGTAAAGCAAGG
PRIMER_RIGHT_3_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=450,22
PRIMER_LEFT_3_TM=59.667
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.5498
PRIMER_LEFT_4_PENALTY=0.333229
PRIMER_RIGHT_4_PENALTY=0.216588
PRIMER_LEFT_4_SEQUENCE=AGCCAAGGAATGAAACGTAAAG
PRIMER_RIGHT_4_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_4=155,22
PRIMER_RIGHT_4=450,22
PRIMER_LEFT_4_TM=59.667
PRIMER_RIGHT_4_TM=59.783
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=10.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_00042 Ps_contig_mira-and-tgicl-ass_franssen_733
SEQUENCE=GTTCGGGGTAAAGCAATATTAGTTTCATCTACAAAACAAGCAAAGAAAGAGAGARTGTTGAGATCCCAGGACTACCACAACCACATTGCAGGAGTCMAGGAAGGGAGCCAAGTGTTCTCTCCGACTTCGACCGCTACCCAAACGCTAGAAATGGCCTTACAACAAGGGTAGGGGTTTGTCTACGATGGAGGTCGGAGGGACTCCCCACCGCCACCACCACCACCACCACCCTGAGATCGTTGGAAGGTAGGGTTGAAGGTTGGGTAGGG
TARGET=211,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00043 Ps_contig_mira-and-tgicl-ass_franssen_734
SEQUENCE=AGCTTATGCTCTGTATGTTTTCCATTTTTGCAATCCAAGACCATTTGGCCTGTTCCTTCGTGGGTTATACTACAATACCAGCTGCTAAACAGAAGTTGAAATGCAGCATTACAAGCCAATGCCGACGCCGTAAACCAAACTCCAAACGTGAGCCAGCCATGAACAAAATTCTCTCCATCACTCTATAAATCAATCTTCACCCCTTCCAGATTCTTCTTCTTCAACCTCATCGCATCTCTAACCAACTTTTCNCCCA
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6283
PRIMER_LEFT_PENALTY=0.237699
PRIMER_RIGHT_PENALTY=0.390638
PRIMER_LEFT_SEQUENCE=CCTGTTCCTTCGTGGGTTATAC
PRIMER_RIGHT_SEQUENCE=TGGGNGAAAAGTTGGTTAGAGA
PRIMER_LEFT=48,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=59.762
PRIMER_RIGHT_TM=60.391
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=4.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.6729
PRIMER_LEFT_1_PENALTY=0.237699
PRIMER_RIGHT_1_PENALTY=0.435201
PRIMER_LEFT_1_SEQUENCE=CCTGTTCCTTCGTGGGTTATAC
PRIMER_RIGHT_1_SEQUENCE=NGAAAAGTTGGTTAGAGATGCG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=251,22
PRIMER_LEFT_1_TM=59.762
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=4.6000
PRIMER_RIGHT_1_END_STABILITY=10.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.6973
PRIMER_LEFT_2_PENALTY=0.306698
PRIMER_RIGHT_2_PENALTY=0.390638
PRIMER_LEFT_2_SEQUENCE=CCAGCTGCTAAACAGAAGTTGA
PRIMER_RIGHT_2_SEQUENCE=TGGGNGAAAAGTTGGTTAGAGA
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.693
PRIMER_RIGHT_2_TM=60.391
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.7419
PRIMER_LEFT_3_PENALTY=0.306698
PRIMER_RIGHT_3_PENALTY=0.435201
PRIMER_LEFT_3_SEQUENCE=CCAGCTGCTAAACAGAAGTTGA
PRIMER_RIGHT_3_SEQUENCE=NGAAAAGTTGGTTAGAGATGCG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=251,22
PRIMER_LEFT_3_TM=59.693
PRIMER_RIGHT_3_TM=59.565
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=1.2171
PRIMER_LEFT_4_PENALTY=0.826416
PRIMER_RIGHT_4_PENALTY=0.390638
PRIMER_LEFT_4_SEQUENCE=TAAACCAAACTCCAAACGTGAG
PRIMER_RIGHT_4_SEQUENCE=TGGGNGAAAAGTTGGTTAGAGA
PRIMER_LEFT_4=130,22
PRIMER_RIGHT_4=255,22
PRIMER_LEFT_4_TM=59.174
PRIMER_RIGHT_4_TM=60.391
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00044 Ps_contig_mira-and-tgicl-ass_franssen_740
SEQUENCE=GATCCTTACACACATAAGGTTCAGCASACCGACGGCGGAATCGACGACGAACGRCGGTGGAAACGGCGGAGCAAGGACGACACAGCGAATCCCAAACCTCAGTGCTGAAACAACGGCGCCGTCTCTGGACGCGCAAAGCTTGACCTTCTCCAAATCGTGGCGCACCAAGCAAAGCAACACCAATTCGCAACTCAAAACCATCGTYGAACCTACCTTCTTGCTTCACCAGATTTAAACAGTAAAATCTAAATGTGGAGGTTGAATCGGAAAACTCACAAAACAGGTATCGCATATTTCTGCTCTGGTTTTCGCTCGATCCTTCTTCTTGAGCTATTCTGAGAGGGCTTTGCGTCGTTGTTGTTCGATGCGTTCTTTTGGTATTGAATCAAGATGAAGCACGTCACGTCGAGTTGCCGAATCGTGATGATTGACGAATCAAGATGGYGATGACGAGTTTGATTTCGTGGAGGATCGCTTCTACTTTTTCGGTTTCGATCTTCTCTCCTYCCGTCGAGGTTCTGYCTGAATTTTGTGAAGTTGCTTAGATTTTCGCAGACTATGGAGGATGGCGAATTGAAGACGTTGATGAGAGAGTGACAGGTTGTGGCTTTACTCCGGTTTCGGTTTCTGTTATATGAAGTTTGTTGAAGGAATTACGGATGGTTACAGGTCCAAGAAGATGATGGTGAAGGAGTTTGTGTGTGGTAGAGAATGAATACGAAGGGTAACGAATGAAGAAATTGGATCACGGTGGTGGTTGGTGAGGGTTGGATTCGATAGCGGTGGTGGTTCTTGGAAGGTTGAAGAAGATTAGGTTGAAGGCGGAGATTGTTACGGTGATGGCTGGGAGAAAGGTTGGTTAGAGATGCGATGAGGTTGAAGAAGAAGAATCTAGGAGATGAGAAGAAGATTGATTTTATAGAGCTAATTTGTGCAATTTGTTGTTGGCTCACGTTTTGCTTTGCGGTTCACCGGCTTGGAAGCGTTAGCTGCGTTTCAATATGGATTTGCAAGTTGTTTGGCTTGGTGGCTTGTGTTCATAGCATCTGGTGTGCGTTTGGAGCTGACTTATGCGTTTTGGTGTTGTTGTAGCTTGCAGCTCATGATGCCTTGTCCTTTTACGTTTCATTTCCTTGGCTTGTCGTTTMMAGTTGTGTTCGGACTTAAACTGTAGCAGTCTTTGAGTTGACGTTAAG
TARGET=879,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00045 Ps_contig_mira-and-tgicl-ass_franssen_764
SEQUENCE=TTTTCTATGTCGGTTTCATCATCCCATGGCTTCACATCCACAAAACAGATGATTTTCCTACTCTCTTCTTTTTTGTCCAGATGCCTTCACAGCTGCTGCCCGTTCCTCAGCTGCCTTCTTCTCTTCCTCAGTCTCTTCACCAAACAAATCCACATCATCATCATCGTCATCCTCTGCTTCGGTGGCCTTTGTGTCAGCAGCTGGGAGGAGTGGCAATGCCTTCTTCAGCCACAAGAGAAGACTTCCCACTAATGACCACCAGACTCCCCTCACCAAGAAACCACCAAGTAAATTCCTACAAACAAAGTCCAATCAATGTNTCTTCGACCCACCTAGACAAACTAGTT
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2504
PRIMER_LEFT_PENALTY=0.131699
PRIMER_RIGHT_PENALTY=0.118715
PRIMER_LEFT_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_SEQUENCE=GTGGGTCGAAGANACATTGATT
PRIMER_LEFT=111,22
PRIMER_RIGHT=331,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.881
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.50
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.75
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.3144
PRIMER_LEFT_1_PENALTY=0.131699
PRIMER_RIGHT_1_PENALTY=0.182658
PRIMER_LEFT_1_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_1_SEQUENCE=TGTCTAGGTGGGTCGAAGANAC
PRIMER_LEFT_1=111,22
PRIMER_RIGHT_1=338,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.183
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.50
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.0500
PRIMER_PAIR_1_COMPL_ANY=5.75
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.3144
PRIMER_LEFT_2_PENALTY=0.131699
PRIMER_RIGHT_2_PENALTY=0.182658
PRIMER_LEFT_2_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_2_SEQUENCE=GTCTAGGTGGGTCGAAGANACA
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=337,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.183
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.50
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3500
PRIMER_PAIR_2_COMPL_ANY=5.75
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.3376
PRIMER_LEFT_3_PENALTY=0.115965
PRIMER_RIGHT_3_PENALTY=0.221594
PRIMER_LEFT_3_SEQUENCE=TCCTCAGTCTCTTCACCAAACA
PRIMER_RIGHT_3_SEQUENCE=AGTGGGAAGTCTTCTCTTGTGG
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.884
PRIMER_RIGHT_3_TM=59.778
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.3533
PRIMER_LEFT_4_PENALTY=0.131699
PRIMER_RIGHT_4_PENALTY=0.221594
PRIMER_LEFT_4_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_4_SEQUENCE=AGTGGGAAGTCTTCTCTTGTGG
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=249,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=59.778
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_00046 Ps_contig_mira-and-tgicl-ass_franssen_788
SEQUENCE=AAGCATGGTAGTCAACGCAGAGTASWCGGGACAACAAACTCTCCATTTCTATTCACACCCCCTACGCTCACATTAGCACAAAACCAAAATAGGCAACCGCAACAGCAACCGTCCGTCTTTGTTCCTTTACTTCATCCTTCATCGGAACTCGTCTCCGTGAAGTTCAATCCAATTCCGGCCGAGTACAAGCCAGATTCGGATTTGCATTACGGAAAGAAAGAAAAGACCGCACCAAAGAAACCATCACGCTCG
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00047 Ps_contig_mira-and-tgicl-ass_franssen_796
SEQUENCE=TCGGGGATTCTAGAACACTTAAAACCCTAACTATATAAGCTAACTTCTACTGTACTTCGTTTTGCTCTTCACTTCTTCTTAGATCTGTGTCTCATTTATTTTCCGATTAGCCAAAAGGTAAAATCCAATGGCCGGAAAAGGAGAGGGACCGGCTATCGGAATCGATCTCGGAACCACCTACTCCTGTGTAGGAGTATGGCAACATGATAGAGTTGAAATCATTGCCAACGACCAAGGTAACAGAACTACACCGTCTTATGTCGCTTTCACTGACTCCGAGCGTCTCATCGGTGATGCCGCTAAGAACCAGGTCGCCATGAATCCTATCAACACCGTCTTCGATGCAAAGAGGTTGATTGGTCGTAGAGTCTCTGATGCATCTGTTCAGAGTGACATGAAGTTGTGGCCTTTTAAGATCATTGCTGGTCCTGCTGAGAAGCCAATGATTGTTGTTAACTACAAGGGTGAAGATAAGCAATTTGCTGCTGAAGAGATCTCTTCAATGGTTCTGATGAAGATGCGTGAGATTGCTGAAGCTTACCTTGGCTCTGCAATTAAGAACGCCGTTGTCACTGTCCCTGCTTACTTTAATGATTCTCAGCGTCAAGCTACCAAGGATGCTGGTGTTATTGCTGGTCTTAATGTCATGCGAATCATCAATGAGCCCACTGCTGCTGCCATTGCATATGGGCTAGACAAGAAAGCAACAAGTGTTGGTGAAAAGAATGTCTTGATTTTTGACCTTGGTGGTGGTACTTTTGATGTCTCTTTGCTTACTATTGAAGAGGGTATCTTTGAGGTGAAAGCCACAGCTGGAGACACCCATCTTGGAGGTGAGGATTTTGATAACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGAAAGAACAAGAAGGACATTAGTGGCAACCCCAGGGCACTCAGGCGATTGAGGACTGCATGTGAGAGGGCGAAGAGAACTCTTTCATCTACTGCCCAGACTACCATTGAAATTGACTCTCTATTTGAGGGAATTGATTTCTACTCCCCTATTACTCGTGCCAGATTTGAGGAGCTCAACATGGATCTCTTTAGGAAATGTATGGAACCAGTTGAGAAATGTTTGAGAGATGCCAAGATGGACAAAAAAAGCATCCATGATGTTGTTCTTGTTGGTGGTTCTACTAGAATTCCAAAGGTTCAACAACTTTTGCAGGATTTCTTTAATGGAAAGGAGCTATGCAAAAGCATTAATCCCGATGAGGCTGTTGCGTATGGAGCTGCTGTCCAGGCTGCAATTTTGAGTGGTGAGGGGAATGAGAAGGTGCAGGATCTTCTCCTTCTTGATGTTACCCCTCTCTCTCTTGGTTTGGAGACTGCTGGGGGTGTGATGACTGTTCTGATCCAAAGGAATACCACCATTCCAACAAAGAAAGAACAGGTTTTCTCAACCTATTCTGACAACCAGCCTGGTGTGTTGATCCAAGTCTTTGAAGGAGAGAGAACAAGGACTAGGGATAACAATTTGTTGGGCAAGTTTGAACTTTCTGGTATTCCTCCTGCTCCCAGAGGTGTTCCCCAAATTACTGTGTGCTTTGACATTGATGCCAACGGTATATTGAATGTCTCTGCTGAAGACAAGACTACCGGGCAGAAGAACAAGATCACCATCACAAATGACAAGGGTAGGCTTTCAAAGGATGATATTGAGAAGATGGTTCAAGAGGCTGAGAAGTACAAGTCTGAGGATGAAGAGCATAAGAAGAAGGTTGAAGCCAAGAATGCATTGGAAAACTATGCATACAACATGAGGAACACCATCAAGGATGACAAGATTGCAGGCAAACTTGATTCTGAAGACAAGAAGAAGATTGAGGACACCATTGAGCAGGCTATCCAGTGGTTAGACAGCAACCAACTTGCAGAGGCAGATGAGTTTGAGGACAAAATGAAGGAATTGGAAAGTGTCTGCAACCCTATCATTGCCAAGATGTACCAGGGTGGTGCAGGTCCTGACATGGGTGCTGCTGCAGATGATGATGCTCCTACTGGTGGAAGTGGTGCTGGCCCTAAGATTGAGGAAGTCGACTAAGTGTAGTTTCTTTCATCCTAACTATGATTTATATTTTCAAGTTATATTTAATTTGATGCATTTGCCGCTTATATTGTTTGCTTTTAGCTGGTTTTTGGAGCTATTTAGGCTACTTCGTTATGTTGTACTGTTAAGGATTGTGTTATTGTTATTGCAATTTTCATATACWATTAAAGGGTTTTAAACTTTTT
TARGET=1338,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00048 Ps_contig_mira-and-tgicl-ass_franssen_797
SEQUENCE=AACGAATGTACCAACGGTGGAGGCTGNCCTGACATGAGTGCAGATGATGATGAAGTTCCTCCAGCTGCAGGTGGTGCTGGTCCCAAAATTGAAGAAGTTGATTAAGTTTTTTCGTTTGATTGATTAGTCTCTTTTAATTTTACGCTTGTTTTAGTTAATTGGTCAGCGTTTTGGGGATTTCCTTTCTGATAATGTGATGTTGTGATGATTATTAGGTGTTTTGTTCGAATTTTGAATTGTTCATCTCAATTACAATTGGATGCTAAGTATTTGTATGAATTTTATTTTTTAATTGGAAGGAAGGAATTTTACTGGACBAACC
TARGET=295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00049 Ps_contig_mira-and-tgicl-ass_franssen_814
SEQUENCE=AAGCAGTGGTATCAACGCAAGAAGTAACGTCGGGGATCAACAATCCAACCTTTCAGCAACACTGGTTACACACTTTACTCTTTTCACTCTCTCTCTCCATCACTTACGTTATCCTTATTCTTTGTTTTCCCACCACATCAATGGAAGTACTCTCTTCAAACAAAAGAAACCAACCTACCTCCTCGTTGAANCCCAACCGCTCCGATGTTCGTT
TARGET=87,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4404
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.400488
PRIMER_LEFT_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_RIGHT_SEQUENCE=ACGAGGAGGTAGGTTGGTTTCT
PRIMER_LEFT=41,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.400
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.6038
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.400488
PRIMER_LEFT_1_SEQUENCE=CCTTTCAGCAACACTGGTTACA
PRIMER_RIGHT_1_SEQUENCE=ACGAGGAGGTAGGTTGGTTTCT
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=60.400
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.7817
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.741841
PRIMER_LEFT_2_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_RIGHT_2_SEQUENCE=AACGAGGAGGTAGGTTGGTTTC
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=186,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.742
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.8973
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.857451
PRIMER_LEFT_3_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_RIGHT_3_SEQUENCE=CGAGGAGGTAGGTTGGTTTCTT
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=60.857
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.9452
PRIMER_LEFT_4_PENALTY=0.203343
PRIMER_RIGHT_4_PENALTY=0.741841
PRIMER_LEFT_4_SEQUENCE=CCTTTCAGCAACACTGGTTACA
PRIMER_RIGHT_4_SEQUENCE=AACGAGGAGGTAGGTTGGTTTC
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=186,22
PRIMER_LEFT_4_TM=60.203
PRIMER_RIGHT_4_TM=60.742
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_00050 Ps_contig_mira-and-tgicl-ass_franssen_815
SEQUENCE=CGGTTTAAACTTTTACGGTTTTTTACGGGTTACCCGTTTAGGGGGTACCCCCGTTTCCTAAACCCCCTAACGAAACGTTAGGCCTTCGTAAAAGACTTGAAAACTACACCACCACCCTCCAAGATRMYGGACGSKARWAGWTCAATTAAATGAGAAGCACGTCGATTCCGACAACACCGACGACACCGGTGACTCCGACAACACCTTCATCGGCGCGTAAAGTTGACAACGTTTGGAGGAGCGTGTTCAATCCTGGGAGTAACTCTGCTACAAAGTCAATTGGTGCTCATGTTTTCGACAAACCACTCCCCAACACCCCCACTGTCTATGATTGGATGTACAGTGGGGATACGAGAAGCAAGCACCGTTGATTAGTGATCAAGTGGATACCAACTCCCTTGGGTTTTGTCATGTAAATAGTGTTCTTGTTTTGTTTCTATCTTCTGCCACGTGTCTACATCACCGTTACGTGTTGAGATCTGTAGTTGGTTTGGTTTGGTCGAGTAGGTTTTAGTTTTTTTTTTAAGTTTTTTAAGTTAC
TARGET=486,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00051 Ps_contig_mira-and-tgicl-ass_franssen_816
SEQUENCE=GAACACTCTTATTACATAAATTTAACTCATCAAACTCTTTTATCAAACAAACAAAATGTTAGATAAACTTTGGGATGATGTTGTTGCTGGCCCTCAACCAGAACGTGGCCTTGAAAGCTTAGAAACTCACCACCACCCTCAAAGATGATGGAAGATGGTGCTACTTATTCTCAGGCTGAACCAACAAAACCNGTATGGTGTGAGAGAAAACAGCCGTAGACAAGAAGAAATATGGANAATGAATCAATTACCTGACTAAAAACG
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00052 Ps_contig_mira-and-tgicl-ass_franssen_825
SEQUENCE=AGAGAAGGAGGAATATGAAGCGGCCCGGCGCGAATTATTGGAAGAAGTAGCTAGGGGTACCTCTGTACAAGATATTCGTGCAAGGTTGACTAACAAAACTAGTGATGTTGAAGTAAAGGAGCCTTCTGTTTCGAAAACAAAGACCATCCCTGATGAACTTGTACAGATTCAAGCATTTATACGCTGGGAAAAAGCTGGGAAGCCTAATTATTCTCCCGAACAACAACTTATTGAATTTGAGGAAGCAAGAARAGGATTGCTAAAGCAAAATCCGAGGCCGCAGAGTACCAAAAACTTCTTGCCTCCAGATTGAAGGAGCAGAGGGACCGCAGAAGTGAGAGTTTGGTTAAGAAGAGATCAAGGCTCTCTACCGCCAAGCCTGTTGCAGCATAGGTCTTTCTTTGCAACAAATGAAGTTCATACTTCGGCCGGTTAGTTTTTGTTAAGTTATTTATTTATTTTTTGGTTCG
TARGET=448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00053 Ps_contig_mira-and-tgicl-ass_franssen_834
SEQUENCE=AATTCCAACAGCATTCATGGATTTATTACTTAGGACCCAAACGTCCCTTTGTAGGGCCACAAATCTGCTCCTCGCCCATGGCAGACATGACAGACACAATCAGATCTTTACCTTCGTTGAATCCATCCTTGATCTGCGAAAGCAGACTATCATCGGACGGAAGCTTAAGGTCATCCTTTGTACCACCGCTCTCAGTCAGCAGACTCACAAATCCATCCTCTGAGATATCAATAAGCTGGTAATCAGTACGATTGACATGAGGAACATCACTAGTTATGAAGAAGAAGAACAATNTCTNATCTTTGCNGTAGAAGTNTT
TARGET=278,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00054 Ps_contig_mira-and-tgicl-ass_franssen_839
SEQUENCE=AAGCAGGGTAGTCAACGACAGAGTACGCGGGGTGGTGAAGAACAAGAAGTAAAACAATGTCTGACGAGGAACACCATTTCGAGTCCAAAGCTGATGCTGGAGCATCCAAGACATATAACCAAACAAACAAAGCTGGAACCATTCGACAAAAATGGTCACATTGTTATACAAAAACAGACCCTGCAAGGTTGTTGTAGGTTCAACTTACAAAAAC
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5645
PRIMER_LEFT_PENALTY=0.361206
PRIMER_RIGHT_PENALTY=0.203343
PRIMER_LEFT_SEQUENCE=AAACAATGTCTGACGAGGAACA
PRIMER_RIGHT_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT=51,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=59.639
PRIMER_RIGHT_TM=60.203
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.9430
PRIMER_LEFT_1_PENALTY=0.361206
PRIMER_RIGHT_1_PENALTY=0.581808
PRIMER_LEFT_1_SEQUENCE=AAACAATGTCTGACGAGGAACA
PRIMER_RIGHT_1_SEQUENCE=GACCATTTTTGTCGAATGGTTC
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=156,22
PRIMER_LEFT_1_TM=59.639
PRIMER_RIGHT_1_TM=60.582
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.9450
PRIMER_LEFT_2_PENALTY=0.741655
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GCAGGGTAGTCAACGACAGAGT
PRIMER_RIGHT_2_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=201,22
PRIMER_LEFT_2_TM=60.742
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=1.0100
PRIMER_LEFT_3_PENALTY=0.806635
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=ATGTCTGACGAGGAACACCATT
PRIMER_RIGHT_3_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=201,22
PRIMER_LEFT_3_TM=60.807
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=1.0100
PRIMER_LEFT_4_PENALTY=0.806635
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=AATGTCTGACGAGGAACACCAT
PRIMER_RIGHT_4_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=60.807
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_00055 Ps_contig_mira-and-tgicl-ass_franssen_851
SEQUENCE=CAATNAGGAGCTAGAGAAAATTAGATGGTTCTGTTTTCTCAATACAAGTTTAGAAGAAAAAAATAAAGCAAGAAGTGTACAAAATTAACAATGGCACATGGTCGTACATATATATATAGATATAGATATAATTAACAAAACCTTATTTTGGCTCTTACTTCCAGTTATTGGCAACAATGTTCAGCCAAATCAACAACCCTTTGTGAGTAACCCCACTCATTATCATACCAAGCAATCACCTTAACCAAGTCATCGCCCATGACCATTGTCACCGCCACCGATGTAAACCAAGGTACTGAATCTATCCAACCAACAAACATGGATCAGCAAACTGCTGTAGATGAGTCTGTTAAACCCAGTTCAACTACATCAGTTCTTTGTGAACTCAGAACCATTGGCGGGAAAAAA
TARGET=108,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1733
PRIMER_LEFT_PENALTY=1.135941
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=TAACAATGGCACATGGTCGTA
PRIMER_RIGHT_SEQUENCE=CAGTTTGCTGATCCATGTTTGT
PRIMER_LEFT=85,21
PRIMER_RIGHT=333,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=1.1953
PRIMER_LEFT_1_PENALTY=1.157991
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=TTAACAATGGCACATGGTCGTA
PRIMER_RIGHT_1_SEQUENCE=CAGTTTGCTGATCCATGTTTGT
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=61.158
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=1.4117
PRIMER_LEFT_2_PENALTY=1.135941
PRIMER_RIGHT_2_PENALTY=0.275749
PRIMER_LEFT_2_SEQUENCE=TAACAATGGCACATGGTCGTA
PRIMER_RIGHT_2_SEQUENCE=TCCATGTTTGTTGGTTGGATAG
PRIMER_LEFT_2=85,21
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.724
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=1.4117
PRIMER_LEFT_3_PENALTY=1.135941
PRIMER_RIGHT_3_PENALTY=0.275749
PRIMER_LEFT_3_SEQUENCE=TAACAATGGCACATGGTCGTA
PRIMER_RIGHT_3_SEQUENCE=CCATGTTTGTTGGTTGGATAGA
PRIMER_LEFT_3=85,21
PRIMER_RIGHT_3=321,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.724
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=1.4303
PRIMER_LEFT_4_PENALTY=1.392962
PRIMER_RIGHT_4_PENALTY=0.037339
PRIMER_LEFT_4_SEQUENCE=ATTAACAATGGCACATGGTCGT
PRIMER_RIGHT_4_SEQUENCE=CAGTTTGCTGATCCATGTTTGT
PRIMER_LEFT_4=83,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=61.393
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_00056 Ps_contig_mira-and-tgicl-ass_franssen_859
SEQUENCE=CCGTTTTCGGTTCTTCGTTGGAGGTTTTGGTTCTAGTGGAGTGATTATTNACAGAGCTGTTGCGAAATACTGAAATTTAGTCAGGTTCAAATGCTTATTCAAGTGTAAGTGTTGCTTGGTTGGTTGGTTAGTTAGAAGTGAGGAAGAAAGATTGCAGGGTTAGCTTCGAGGGTTCAAGAGAAGGGGTAAAAGGGCCGTTGAGTATCTACTGCTGAGGTATTCGAGTTAACCCCGTCTTTGGTTGTTGTTGAAGTGAAGAAAAAAGGAGGGGATAAAGTTGAGTATGATAGGTTTTTAAACACTGAATTGAAGCCTGCTTTGCATAGTTTAACCATGGAAGAATCTGCAGGTTCTTCATGTCAAAATACACCAGAGGAAACTTTGCAACAACGCGTCGTTTTCTGATTCCGCCATTGACAAACNTTCAGATAGCATTGAATCTCTA
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4912
PRIMER_LEFT_PENALTY=0.376728
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=TTGGAGGTTTTGGTTCTAGTGG
PRIMER_RIGHT_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT=17,22
PRIMER_RIGHT=157,22
PRIMER_LEFT_TM=60.377
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.5297
PRIMER_LEFT_1_PENALTY=0.415225
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=ACAGAGCTGTTGCGAAATACTG
PRIMER_RIGHT_1_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=157,22
PRIMER_LEFT_1_TM=59.585
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=0.5546
PRIMER_LEFT_2_PENALTY=0.440098
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=CGTTGGAGGTTTTGGTTCTAGT
PRIMER_RIGHT_2_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=157,22
PRIMER_LEFT_2_TM=59.560
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.5693
PRIMER_LEFT_3_PENALTY=0.415225
PRIMER_RIGHT_3_PENALTY=0.154110
PRIMER_LEFT_3_SEQUENCE=ACAGAGCTGTTGCGAAATACTG
PRIMER_RIGHT_3_SEQUENCE=GGTTAAACTATGCAAAGCAGGC
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=332,22
PRIMER_LEFT_3_TM=59.585
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.5737
PRIMER_LEFT_4_PENALTY=0.459263
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=GAGCTGTTGCGAAATACTGAAA
PRIMER_RIGHT_4_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=157,22
PRIMER_LEFT_4_TM=59.541
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00057 Ps_contig_mira-and-tgicl-ass_franssen_892
SEQUENCE=CCCCTCCGTTGTCGTGGTCGTCNGAAGACANGGTAGTCAACGACCACGAGTAACGCGGGGAGGTCCACTCCAATCATAATAATACTTTCCTTGTTTCTAACCAGAAAAAAACGAAACAASTCGGGGGATAGATCACTCAATCACTGCATATACATATCTTCATTGAATTAATCAACATTACTCTCTCTTCCTCCATCTTCTAGATTCTTCCATCATCAACAACAACAACAAACATGGCTGCTGCAACCGTTTATCA
TARGET=217,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00058 Ps_contig_mira-and-tgicl-ass_franssen_895
SEQUENCE=TGATGGTGCCTACCCTTCCACTTATAGAAACTTATTTGTTTTCTAATGAAACCTTTTTGAAGACAAGTCTCTCTGGCGTKCTAACAATAGATGTTCTGATCGGAAGGGAAGTGGAGTTGGAGTATGGCAAGGAGACTGCGGCTGTTACTGCAGCAGCCATGTTTGTTGTTGTTGTTGATGATGGAAGAATCTAGAAGNTGGAGGAAGAGAGAGTAATGTTGATTAATTCAATGAAGATATGTATATGCAGTGATTGAGTCCCTGCGTACTCTGCGTTGATA
TARGET=163,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00059 Ps_contig_mira-and-tgicl-ass_franssen_901
SEQUENCE=AGGTCTAAGAGGTAAAAGAAAGTACTAGTAGTAGTAGTAAGTTTACCTTCCACTTAGTTTCTTACCGTAAGACCCCCTCCCGGACGTACAGCATTGTAAGAACGTTCGGTTCCAAACCGGTAGGGAAGTACAAGAAGTTCAGATYWGYTKCAATTCTTTGCAGTGCGCTCGCAAGCCATCCTGATATACCGTCCTGATTCTCGTTCCCGTGCATGCAAGCACTTTTCTATTAGCTTTTTTTGTTTTCCTTTAGCTTTTTTCACTGCTCTCCTTCAAACAACATCTTTCCAAATCCTCTCCTTGCCACTATAATTTTTGTTTCTCTCCTTGTTTCTTTTGTATTTCTTTCTGTTTGAACTGTGGCCATGGCAGTTTCTGCAATTGGTTTTGAAGGTTTCGAAAAGAGGTTGGAAATATCCTTTTCTGACCCAGGACTTTTCTCTGACCCTCAAGGAAGGGGATACGATCTCTCACAAATCCCAATTGGACGAGATTCTTGCACCNGCTGAGTGCCACTATTTGTTTCCTTCCCTTGACTAAAGTGGAGGTATTGGTTTAGTACTTT
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00060 Ps_contig_mira-and-tgicl-ass_franssen_907
SEQUENCE=GGKTTCCGTCTGATCGAGTGTGGTTGTGGGGTTTTAGGGCGCAATCTTCTTTAGCTAGCACGCAGAGCAGACGCAACCTTCCTCACCTCTCTTCCATTCACGATGGCCGRCGATGACTACGAAGATATCGACATGGGATACGAAGAGGAGCCAGCGGAGCCTAGARATCGAGGAAGGCGCAGGAGGAGGAGGATCCAGAATAAACAAAAACGNGTRGWSWYRGAWTAGGG
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00061 Ps_contig_mira-and-tgicl-ass_franssen_910
SEQUENCE=CCTTTTCCCTAAACTTCCCTTCCTTCCTTGTCCACTTATTAATTTTTGTTTTCTTCTTCCTTTGTTTTCTTTTTGTTATTTTCTTTTCTTGTTTTGTAACTTGTTGGCCATGGCAGTTTCTGCAATTGGTTTTGAAGGTTTCGAAAAGAGGTTGGAAATATCCTTTTCTGACCCAGGACTTTTCTCTGACCCTCAAGGAAGGGGATTACGATCTCTCACAAAATCCCAATTGGTACGAGATTCTTGCACCAGCTGAGTGCACCATTGTTTCTTCCYTTGCAAATGAGGATGTTGATTCCTATGTTCTATCTGAGTCCAGCCTTTTTTGTTTATGCCTACAAGCTCATAATCAAACCTGCGGCACTACCAAGTTACTGCTTTACAATCCCAACAATTATTAGAAAATTTCGGGCCCGAAACCTTCCCTAATTTTTCCTCTTTAAATTGGTTTAAGATTCTTGGTTAGGAAGGGTACCCACCCCGGAGGGCCTACG
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00062 Ps_contig_mira-and-tgicl-ass_franssen_934
SEQUENCE=TACACTCCCTTCACGGTTACCTCAGACTTCTTACAAATCTAGTTTCCACCGATTCCACCTCCCTTCATTTTGAGTATGTCGTTTTTTTATTCTCTTGCACAACCTTCACAATGCATGTGAACTTTAAGAACAACCGTTACAATCTGAGGCTCTTCTTTCTTCTCCTCTTCAGGTTTCTTGGTTTTCTCTTCTTCCTCAACAACTTTTTCCTCCTCTGGTGGATTCGGGAGCGGAGATACAAGCTCAACCTTCCTCCGACTCTTCGTCTGAATTCTCTCCACACCTTCGCCGGNTCAGTCTTTCCTCCTACCTTTCAACCAACCAACCTCATTGTTGTTTTGCTAGTTTAGTTGTCCACCTACCCTTACCAACCGGTACCGGGTAATTCTACAACAGCGACGTACTTCCGANCCACACGTCCTTACAACAATACG
TARGET=315,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2048
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=ACAACCGTTACAATCTGAGGCT
PRIMER_RIGHT_SEQUENCE=GTTGGTAAGGGTAGGTGGACAA
PRIMER_LEFT=129,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.2048
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=ACAACCGTTACAATCTGAGGCT
PRIMER_RIGHT_1_SEQUENCE=TTGGTAAGGGTAGGTGGACAAC
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=370,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.2808
PRIMER_LEFT_2_PENALTY=0.136881
PRIMER_RIGHT_2_PENALTY=0.143968
PRIMER_LEFT_2_SEQUENCE=CAACAACTTTTTCCTCCTCTGG
PRIMER_RIGHT_2_SEQUENCE=GTTGGTAAGGGTAGGTGGACAA
PRIMER_LEFT_2=196,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=60.137
PRIMER_RIGHT_2_TM=60.144
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.2808
PRIMER_LEFT_3_PENALTY=0.136881
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=CAACAACTTTTTCCTCCTCTGG
PRIMER_RIGHT_3_SEQUENCE=TTGGTAAGGGTAGGTGGACAAC
PRIMER_LEFT_3=196,22
PRIMER_RIGHT_3=370,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.2861
PRIMER_LEFT_4_PENALTY=0.142170
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=TTCTCCTCTTCAGGTTTCTTGG
PRIMER_RIGHT_4_SEQUENCE=GTTGGTAAGGGTAGGTGGACAA
PRIMER_LEFT_4=159,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_00063 Ps_contig_mira-and-tgicl-ass_franssen_935
SEQUENCE=CTGCATGTCCTCCATACTATTCTTTAAACTTATTATTCTTTCCACCAGCACCGCAGATCTTCAGCGACGAGAATCCAAATGCTTGCAGTGTTATGTGAAATTTAATTTCACAAATATCAAGGGTCATATTAAAAATAAGAGATAGTAATTAGTTTTATTTTGCTAAATATACTAGAGTATACATGAACCATGGWAWTTTTTATTTTGTAAKATTTTTATACTTTTGTTGAAGTGAGTAATTTCTTATTTTKKTTTAAAGGCATGTGAGAAGTTAGGAAAGTTTGTTATGATATAACAAACAAACAAGCATCTATCTTAGATAGGAAANCTATTTATGTTACTTTTTAAAGTTTT
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00064 Ps_contig_mira-and-tgicl-ass_franssen_965
SEQUENCE=CAGCCTGATCAGGCAAGGATTTTAGGGTTTTGAATGCTTCGTACTACCTCAAAGCTGGAGGTCATTTTTGTTTATTTTCTTATTCAAGGCCAATTGTATCGATTCCACAGTCCCTGCTGAGGCAGTGTTCACCAGTGAAGTGAACAAGTTGAAAGCAGATCARATTTAAACCATTTGAGCAAGTGACTCTTGAACCTATTTGAGGAGGGACCATGCTTGTGTTGTTGGTGGCTACAGAATGCTAAGAAGAAAGAAAGATGCTAGAAGTTAACCTTTATTTTTGTTTAGTTTTAAAATTAGTTACTTATTTGTTA
TARGET=247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00065 Ps_contig_mira-and-tgicl-ass_franssen_979
SEQUENCE=CAACTCCTCAATAACATCTTCATTGAAATTTTCTTTCTTTCCCTTTTGAATGTTCTTCTCTTACCTCAGTTTGTAGCTTTTGCTAATACACTTGGGTTTCTTATCATTTCTGT
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00066 Ps_contig_mira-and-tgicl-ass_franssen_999
SEQUENCE=CGAAACCCCGTAAAACCTAACGGAAGTCAGGTCGTGAGCTATCTGGTTGGGTTGGGGGCGGATTCTGTTCGGTTTTTAGGAGTTAAAGGTTCGTATGTCTGAATTAGACGCACAAACTACCTACCTACCTTTGTATCCTTTTGTCTGAGGCAAATAGCTGAGGACGCCGGTGCTGGCTCATCAAAGGAGTACGTGCATATTCGTATACAGCAGCGAAATGGTAGAAAAAGCTTGACAACTGTCCAGGGTTATTTGAAGAAAGAATTCAGTTACAACAAAGATTCTTAAAGACCTTTAAGAAGGAGTTTTGCTGTAATGGTACAGTTGTCCTTAGGACCCAGAGCAAGGACAGGTTATTCAACTTCAAGGTGATCAGAGGAAGAATTGTTTCTACCTTCCTAGTCCAGGCTGGAATTGTGAAGAAGGAGCATTAATTAAAATCATGGTTTMYAAGCTGCGTAGGAAACTTGGAAAGTTGTTTAAGTTTGTATCGTGGCCCACATCGG
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00067 Ps_contig_mira-and-tgicl-ass_franssen_1001
SEQUENCE=GGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGANCTCGAGAGCACTACAGAGAAGAATTAMACCACCTGACTGGCTATTATTGTTTGATATAACNTGTGTAATGAATGAGTTGACTAACTTTAGGGTTAATTTTGGWAGGGTTTCGTTTTTAACCTTTAACCCTAACAAATTAAAACGTTAAAAA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00068 Ps_contig_mira-and-tgicl-ass_franssen_1002
SEQUENCE=GGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTAGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTGATATAACNTGTAGTAATGTAATGAGTTGCYAACTTTAGGGTTATTTKGRAGGGTTTCGTTTTTAACCTTAACCCTAACAAAGTTAAAACGTTAAAAA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00069 Ps_contig_mira-and-tgicl-ass_franssen_1003
SEQUENCE=GGGATGACRAGGAGGAAGGTTAAAGTAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTTATTGTTTGATATAACATGTGTAATGATGAGTTGCAACTTAGGTTATTTGGAGGTTGTTTTACCTTACCTAACATAAAGTAAAA
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00070 Ps_contig_mira-and-tgicl-ass_franssen_1004
SEQUENCE=GGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTTATTATTGTTTGATTAATAACATGTTAGTAATGAATGAGTTGACTAACGTTTAGGTTATTTGGAGGKTTWCGTTTTTAACCTTAACCCTAACAATTAAAACGGTTAAAA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00071 Ps_contig_mira-and-tgicl-ass_franssen_1005
SEQUENCE=GKGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAGGRNCTCGAGAGCACTACAGAGAAGAATTAACCNCCTGCTGGCTATTATTGWTTGWTAATAAACAATGTAGTAANGAATGAGTTTGACCTAAACGTTTAGGTTATTTGGAGGTTCTTTACCTACCTANAATTAAAACG
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00072 Ps_contig_mira-and-tgicl-ass_franssen_1006
SEQUENCE=GGGTATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTRGTGACGATTTCTCAGTTCAGTCTGAGAAGGGNCTCGAGAGCACTACAGAGAAGAATTAAACCNCCTGCTGGCTTATTATTGTTNATANAACNTGTAGTAATGAATGAGTT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00073 Ps_contig_mira-and-tgicl-ass_franssen_1007
SEQUENCE=AAAAGAACAAGTGGTATCAACGCAGAGTGGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTTGATATAACATGTGTAATGAATGAGTTGCTAAACTTTAGGGTTATTTTGGAGGGTTTCGTTTTTAACCTTAACCCTAACTAAATTAAAACGTTAAAAACC
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1311
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT=52,22
PRIMER_RIGHT=167,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.1311
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.1764
PRIMER_LEFT_2_PENALTY=0.175676
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=GAACAAGTGGTATCAACGCAGA
PRIMER_RIGHT_2_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=167,22
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.2112
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.080887
PRIMER_LEFT_3_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_3_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.2112
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.080887
PRIMER_LEFT_4_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_4_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_00074 Ps_contig_mira-and-tgicl-ass_franssen_1008
SEQUENCE=GGGTATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAGGGGCTCGAGAGCACTACAGAGAAAGTAAACTTAAACCCACCTGCTGGCTATTNTTGTTGATATAAC
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8133
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.682954
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT=25,22
PRIMER_RIGHT=156,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.683
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.8133
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.682954
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=156,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.683
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=1.1996
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=0.682954
PRIMER_LEFT_2_SEQUENCE=ATAGCTCAACAGTGGGAGGATG
PRIMER_RIGHT_2_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=156,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=60.683
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=1.3346
PRIMER_LEFT_3_PENALTY=0.651681
PRIMER_RIGHT_3_PENALTY=0.682954
PRIMER_LEFT_3_SEQUENCE=GGGTATGACAAGGAGGAAGGTA
PRIMER_RIGHT_3_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=156,22
PRIMER_LEFT_3_TM=59.348
PRIMER_RIGHT_3_TM=60.683
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=1.5210
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=1.390638
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=CCAGCAGGTGGGTTTAAGTTT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=143,21
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.391
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_00075 Ps_contig_mira-and-tgicl-ass_franssen_1009
SEQUENCE=GGGGTATTGTACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGAGCTCGAGAGCACTACAGAGAAGAATTAACCACCTGCTGGCTTATTATTGTTAGATATAACNTGTNTAATGAATGAGTTGACTAACGTTAGGTATTTGGAGGTTNTTACTACCANATAAGTAA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4775
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=CCAGCAGGTGGTTAATTCTTCT
PRIMER_LEFT=28,22
PRIMER_RIGHT=141,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.4775
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGGTGGTTAATTCTTCT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=141,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.5133
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.382951
PRIMER_LEFT_2_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_2_SEQUENCE=CAATAATAAGCCAGCAGGTGGT
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=151,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.383
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.5133
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.382951
PRIMER_LEFT_3_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_3_SEQUENCE=CAATAATAAGCCAGCAGGTGGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=151,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.383
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.6197
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.489375
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=TAAGCCAGCAGGTGGTTAATTC
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=145,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.489
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_00076 Ps_contig_mira-and-tgicl-ass_franssen_1010
SEQUENCE=AAGCMGTGGTATCAACGCAGAGTGGGATGACTAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAAGAGAAGAATTAAACCCACCTGCTGGCTTATTTATTTGTTTGATATAACNTGTNTAATGAATGTAGTTGACTAACGTTTAGGTTATTTTAGGTAGGTTTACGTTTTACCTTACCCTAACTATAAAGTAAACTTAAA
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00077 Ps_contig_mira-and-tgicl-ass_franssen_1011
SEQUENCE=GGGTATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGANCTCGAGAGCACTACAGAGAAGAATTAAACCNCCTGCTGGCTTATTTATTGTTTGAATAATAACNTGTGTAATGAATGAGTTGACTAACTTTAGGTTATTTTGGAACGGGTTTTACGTTTTTTAAACCCTTTAACCCTAAC
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1988
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.068420
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT=25,22
PRIMER_RIGHT=152,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.932
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.5250
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.1988
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.068420
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=152,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.932
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.5250
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.5851
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=0.068420
PRIMER_LEFT_2_SEQUENCE=ATAGCTCAACAGTGGGAGGATG
PRIMER_RIGHT_2_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=59.932
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.5250
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.7201
PRIMER_LEFT_3_PENALTY=0.651681
PRIMER_RIGHT_3_PENALTY=0.068420
PRIMER_LEFT_3_SEQUENCE=GGGTATGACAAGGAGGAAGGTA
PRIMER_RIGHT_3_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=59.348
PRIMER_RIGHT_3_TM=59.932
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.5250
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.7353
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.604956
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=AATAAATAAGCCAGCAGGNGGT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.605
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.50
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.2250
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00078 Ps_contig_mira-and-tgicl-ass_franssen_1012
SEQUENCE=GGGTATTGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGTAACTTAAACCCTACYTGTCTGGCTTATTTATTTGTTTGACTAATAACTAGTTGTAGTTAATTAGAATGTAGTTGACCTATTTAGGTTAGTTGGTAGGTTTTACGTTTTTACCCTTAACCCGTTAACCAAAGTTAAAAGTTTAAAAA
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00079 Ps_contig_mira-and-tgicl-ass_franssen_1013
SEQUENCE=GGGTATTGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGTATTTCNCAGTTCAGCTGAAGGAAGGAGCTCGAGNGCACTACAGAAAGAAGTAACTTAACCGCTG
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9041
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=1.773775
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=ACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT=26,22
PRIMER_RIGHT=128,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=58.226
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.50
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.0750
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=1.9041
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=1.773775
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=ACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=128,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=58.226
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.50
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.0750
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=2.2904
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=1.773775
PRIMER_LEFT_2_SEQUENCE=ATAGCTCAACAGTGGGAGGATG
PRIMER_RIGHT_2_SEQUENCE=ACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=128,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=58.226
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.50
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.0750
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=2.5679
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=2.437528
PRIMER_LEFT_3_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_3_SEQUENCE=TTACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=130,24
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.562
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.50
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.0750
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=2.5679
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=2.437528
PRIMER_LEFT_4_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_4_SEQUENCE=TTACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=130,24
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.562
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.50
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.0750
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_00080 Ps_contig_mira-and-tgicl-ass_franssen_1031
SEQUENCE=CCTTAATGATTCAGAATTACTTCTATAAAAAAATAAGTATCTGCTGTGTCTTTGGGAATTTTAATCAGTGTGTTTCTGGTGTGATGATCTGATAGCGTTCTGCTTTGATTTTCTTTCTTTCTTATGTAAACTACATTGTGCTGAATAAATGATATTATATGCACATGTATGAATGGCATGTAAGGTATGATATTGAAGAAGTTAACCAAAGTAGGTYAAAAAATTACAAAAG
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00081 Ps_contig_mira-and-tgicl-ass_franssen_1035
SEQUENCE=AAGCAGTGGTACAACGCAGAGTKTTTACTTAAACTTTGTTTGTTAAGGTAAGTAAGTAATATTGTGGTGAGATTAAAGTGGCTCTCACTTTTACACCTGAGAGAAATGAAGAACAGTGGTATCAACGCAGAGGAGAGTGGTGGATGGAAAGAATCAGCTAGTGAATATTGAAGTGAATCAAGGATTTGCATTTACAGATTTCCAAGGTCTAAAATTACTGCTACAAAAAAATCTCTTCAGTCCCTTTAAGAATTTTAATCAATGTGTTTTCTGGTGTGATGATATGATAGTGTTCTGTTTTTACTTTCAACCAATTTGATATATAAACTATATTATCCTTGTTATAACATTGTACGCACTAAAATTTATTCTGATAACAGAACACGTTCAACAAATTTCTGAATGTATAGATATAATCAAATAAACACTGTCAAGACCTTATTCAAATTCGAAAACAGAAACAGTGAAATAAGAGTATATAG
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00082 Ps_contig_mira-and-tgicl-ass_franssen_1036
SEQUENCE=AAGCAGTGGTTCAACGCAGAGTTTTTACTTAAACTTTGTTGAAGGAAGAAGAATATTGTGGTGAGATTAAAGTGGCTCTCACTTTTACACCTGAGAGAAATGAAGAACAGGGTTACAATGAAGAGGAGAGTGGTGGATGGAAAGAATCAGCTAGTGAATATTGAAGTGAATCAAGGATTTGCATTTACAGATTTCCAAGGTCTAAAATTACTGCTAACAAAAAAATCTTCTTCAGTACCCCTTTAAAGTAAATTTTAATCAATGTGTTTTCTGGTGTGATGATATGATAGTGTTTCTTGTTTTTAACTTTACCAAACCAATTTTAGGTATTAATTAATTAAAAACCTTAATTATTACTTCCTTTAGGTTTAATTAAACCTAATTGGTTACCGGACCTAAACTTTAAAAATTTTAATTTCTTA
TARGET=329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3275
PRIMER_LEFT_PENALTY=0.361394
PRIMER_RIGHT_PENALTY=1.966130
PRIMER_LEFT_SEQUENCE=AGTGGTGGATGGAAAGAATCAG
PRIMER_RIGHT_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT=128,22
PRIMER_RIGHT=402,22
PRIMER_LEFT_TM=60.361
PRIMER_RIGHT_TM=58.034
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=2.4173
PRIMER_LEFT_1_PENALTY=0.451213
PRIMER_RIGHT_1_PENALTY=1.966130
PRIMER_LEFT_1_SEQUENCE=GGTGGATGGAAAGAATCAGCTA
PRIMER_RIGHT_1_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_1=131,22
PRIMER_RIGHT_1=402,22
PRIMER_LEFT_1_TM=60.451
PRIMER_RIGHT_1_TM=58.034
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=2.4768
PRIMER_LEFT_2_PENALTY=0.510631
PRIMER_RIGHT_2_PENALTY=1.966130
PRIMER_LEFT_2_SEQUENCE=ATGAAGAGGAGAGTGGTGGATG
PRIMER_RIGHT_2_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_2=117,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=60.511
PRIMER_RIGHT_2_TM=58.034
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=2.5098
PRIMER_LEFT_3_PENALTY=0.543622
PRIMER_RIGHT_3_PENALTY=1.966130
PRIMER_LEFT_3_SEQUENCE=ACAATGAAGAGGAGAGTGGTGG
PRIMER_RIGHT_3_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_3=114,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.544
PRIMER_RIGHT_3_TM=58.034
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=2.5232
PRIMER_LEFT_4_PENALTY=0.557021
PRIMER_RIGHT_4_PENALTY=1.966130
PRIMER_LEFT_4_SEQUENCE=GAGAGTGGTGGATGGAAAGAAT
PRIMER_RIGHT_4_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=402,22
PRIMER_LEFT_4_TM=59.443
PRIMER_RIGHT_4_TM=58.034
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_00083 Ps_contig_mira-and-tgicl-ass_franssen_1066
SEQUENCE=CCTTTTTTGTGACTATGTTCATCACTATCATCTGGAGACTTGTCCCTTCTGCTTCTTCTGTGCTCCCTCTTTTTCTTCCTCCTCCTCCTTTCACCGGAATCATCCGAATCACTAGCTGCCCTATTAGTCTTAACACTCCTCTTCTTTGCTCTGCCTCTCTTTCTCCTCTTCCGGTCAGAATCTTTGTCTGACTCATCATCATCTGAGTCCTCCTTCTTTCTATAAGAACCTCTCTTCCCACTAACTTTCTTCCCTGACCTTTCAGCAATAKYCMTCTMRRTTTTTCAGCCAATGGATTCGCCAAAGGTGGAACTTTAGCAAGAAACTAAAGAAGCACCACCACCATTAGTTTCTACTTTAGAACTCATTCTGTCGGAAGTTGAAGTTGAATAATTGAAGAAACGGTGAAGAGGGAGATTAGAAAGGAAAAGGAGAATCTGGATTTGGAAACATACGTGAAGATATTAGAAGGAATGGAATTGCTAGGCCACGAAAGAGTATCAAAAGATATGATGCTAAATGATAGCTCAAGTGAGAGTGAGGCACAGAAAGAGAGAAAGGGGTGTGTGTGGAGTTTGTGAATGATCAAAAGAAGTGGCSMMRATNTCTTAG
TARGET=77,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00084 Ps_contig_mira-and-tgicl-ass_franssen_1071
SEQUENCE=ATGTTAACTAAATTTTATTTTCAATAAGATAAGAAACAGATGCATTGATTAAGAGAGACGACACTCTACAATAATAATAATAACACAGTTAGAAACTCGACACAGCATACTATAGTACAAATATAAGTAAAGTTATTGTTTTCGTTTTTATTCATGACTATACTCTTCTCTAATTAGTATTACAATGACTTATTAATTATTA
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00085 Ps_contig_mira-and-tgicl-ass_franssen_1093
SEQUENCE=GAATCATTAATCACCACCTTTATTTTATTCATTTTCGCATCGAAACTCACATGGCCAATTATAGTATTTAATTATTTATAATTATTATATTTATTKACGTACTATATTTATTTATTTTCGTGGACTCTATTCTCTTGACCATCCCCATTGTTTTTCTTCATCGTTCTTCTTTCTATTCCAACAATTTTCCGATTTCGAACGATATCATGGAAGAAGTTAACGTTGTTGAAACTAAGGATGGAACCGTTTCGGTAGCTACTGCGTTTGCTGGTCACCAGGAAGCTGTGCAGGCTAGAGACCACAAATTTTTAAGAAAAGCAGTTGAAGAAGCATATAAAGGGGTAGATTGTGGAGATGGAGGCCCTTTTGGTGCTGTTATTGTTCACAACGATGAAGTAGTTGCTAGTTGTCACAACATGGTTCTGAATTACACCGATCCAACTGCTCATGCAGAGGTCACAGCAATCAGAGAGGCTTGTAAGAAGCTTAAGCAGATAGAACTTTCAGATTGTGAAATATATGCTTCTTGTGAACCCTGCCCCATGTGCTTTGGTGCAATCCACCTCTCGCGGATTAAGAGATTGGTTTATGGAGCAAAGGCAGAGGCAGCAATCGCTATTGGGTTTGACGATTTTATTGCAGATGCATTGCGAGGTACTGGATTCTACCAGAAAGCAACGTTGGAAATTAAAAGAGCTGATGGTAATGAGGCCATGATTGCAAGAAGGTTAGGTTTTWGAAGAAAAACAAAGGACTAAAAACTTACCTAAAATGTTAAGTTTGAATTATTACCTAATTTCTTTTACACAACATTAAGTGAACTAATCTACGACCGTAAACTTAAACCGTTTACGTTTAACGTAACGTTAAACGGGGTACCGGGGTTAACGTTTTAAGGTTTAA
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00086 Ps_contig_mira-and-tgicl-ass_franssen_1119
SEQUENCE=TACTTGATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACTAAGTTTGAACTTGATACAAAGGTTTGTTTCTTCTGAATTAAGGAAAGTTATAGGAAAGAATTACTAAAAGGTTTAAAAAACCGTTTCTTAACATCTAATTGTTTAGGGTAAGGTTTTCGTTTACCGTTTAGTAATACTAA
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5485
PRIMER_LEFT_PENALTY=1.065617
PRIMER_RIGHT_PENALTY=0.482886
PRIMER_LEFT_SEQUENCE=TACTTGATTTCATTTGGTGGGC
PRIMER_RIGHT_SEQUENCE=ACGGTAAACGAAAACCTTACCC
PRIMER_LEFT=0,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=61.066
PRIMER_RIGHT_TM=60.483
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=1.6594
PRIMER_LEFT_1_PENALTY=1.065617
PRIMER_RIGHT_1_PENALTY=0.593822
PRIMER_LEFT_1_SEQUENCE=TACTTGATTTCATTTGGTGGGC
PRIMER_RIGHT_1_SEQUENCE=CGGTAAACGAAAACCTTACCCT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=61.066
PRIMER_RIGHT_1_TM=60.594
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=1.7962
PRIMER_LEFT_2_PENALTY=1.313269
PRIMER_RIGHT_2_PENALTY=0.482886
PRIMER_LEFT_2_SEQUENCE=GATTTCATTTGGTGGGCTTCT
PRIMER_RIGHT_2_SEQUENCE=ACGGTAAACGAAAACCTTACCC
PRIMER_LEFT_2=5,21
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=60.313
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=1.9071
PRIMER_LEFT_3_PENALTY=1.313269
PRIMER_RIGHT_3_PENALTY=0.593822
PRIMER_LEFT_3_SEQUENCE=GATTTCATTTGGTGGGCTTCT
PRIMER_RIGHT_3_SEQUENCE=CGGTAAACGAAAACCTTACCCT
PRIMER_LEFT_3=5,21
PRIMER_RIGHT_3=186,22
PRIMER_LEFT_3_TM=60.313
PRIMER_RIGHT_3_TM=60.594
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=2.3059
PRIMER_LEFT_4_PENALTY=1.065617
PRIMER_RIGHT_4_PENALTY=1.240245
PRIMER_LEFT_4_SEQUENCE=TACTTGATTTCATTTGGTGGGC
PRIMER_RIGHT_4_SEQUENCE=CGGTAAACGAAAACCTTACCC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=186,21
PRIMER_LEFT_4_TM=61.066
PRIMER_RIGHT_4_TM=59.760
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00087 Ps_contig_mira-and-tgicl-ass_franssen_1120
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTTACTTGTATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACAAGTTTGAACTTGATCAAAGGTTGTTTCTTCTGATTAGGAAAGTATGAAGATCAAAGTTTAAAAACGTTTCTTACATCAATTGTTTGGAAGGTTTTGTTTACCGTTTGTAATACTAATGCGGATCTTCGCGATCCAATGATTAGTTTAAAAACAATGTTGTACCTTGAAGTTAACTTTTGGCAAGAAATGATCAAACA
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3281
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.212376
PRIMER_LEFT_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_SEQUENCE=CAAACGGTAAACAAAACCTTCC
PRIMER_LEFT=24,22
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=59.788
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.3281
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.212376
PRIMER_LEFT_1_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_1_SEQUENCE=CGGTAAACAAAACCTTCCAAAC
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=187,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=59.788
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4491
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.212376
PRIMER_LEFT_2_SEQUENCE=CTTGTATTTCATTTGGTGGGCT
PRIMER_RIGHT_2_SEQUENCE=CAAACGGTAAACAAAACCTTCC
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=191,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.788
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.5747
PRIMER_LEFT_3_PENALTY=0.115769
PRIMER_RIGHT_3_PENALTY=0.458951
PRIMER_LEFT_3_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_3_SEQUENCE=AACTAATCATTGGATCGCGAAG
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=229,22
PRIMER_LEFT_3_TM=60.116
PRIMER_RIGHT_3_TM=60.459
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.6956
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=0.458951
PRIMER_LEFT_4_SEQUENCE=CTTGTATTTCATTTGGTGGGCT
PRIMER_RIGHT_4_SEQUENCE=AACTAATCATTGGATCGCGAAG
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=60.459
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_00088 Ps_contig_mira-and-tgicl-ass_franssen_1122
SEQUENCE=CGTTTCTTTAATTTTGTATTTTTTGGCAGAGAGTTACTGATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACAAGTTTGAACTTGATCAAAGGTTGTTTCTTCTGATTAGGAAAGTATGAAGATACAAAGTTAAAAACGTTTCTTACATCAATTGGTTTAGGTAAGGTTTTGTTTACCGTTTGTAATACTAATGCGGAGTTCTTCGCGATCCAATGAATTAAGTTTTAAAAAMMAATTAGTTGTACCTTGAAGTACTCTTGGACAAGAAATGAA
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00089 Ps_contig_mira-and-tgicl-ass_franssen_1125
SEQUENCE=TTAAGTCTTAGTTGGTTATTCAACGCAGAGTTACTTGTATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTTCAACAAGTTTGAACTTGATACAAAAGGTTGTTTTCTTCTGATTAGGAAAGTATGAAGATCAAAGTTAAAAACGTTTCTTACATCAATTGTTTGGAAGGTTTWGTTTAACCGTTTAGTAAATACTAATGCGGAGTTCTTCGCGATCCAATGATTAGTTTTAAAAACAAATGTTGTACCTGAAGTTAACTTTTAGACAAGAAATG
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00090 Ps_contig_mira-and-tgicl-ass_franssen_1134
SEQUENCE=TTCCTTCAGCTTGCGTCGGAGACANGGTGATGGCCACCGTGAAAGAAAGGGGAAAAAGGACCCAGAATCTGTAGGGAAAAAAGGTTTTAGCCTGCTGTCATCGTTCGTCAACGTAAGCCATGGCGCCGAAAGGACGGTGTCTTCATGTACTTTGTAAGATAATGCTGGTGTCATTGTGAATCCTAAGGGTGAAATGAAAGGTTCTGCCATCACTGGTCCAATCGGTAAGGAGTGTGCTAGATACTAGTCGGACCGAAGGTATTGACTAAGTCGTACCTAAACCTAAGTCCGACCTTAAGTTTAGTTAAGTAGGTAGGTAGTAACTTACGTTTAACGGTTTAAACTACCGACCCTAGTTTGTTT
TARGET=309,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6718
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.671790
PRIMER_LEFT_SEQUENCE=GCTGGTGTCATTGTGAATCCTA
PRIMER_RIGHT_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT=163,22
PRIMER_RIGHT=352,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.328
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.6761
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.671790
PRIMER_LEFT_1_SEQUENCE=AATGAAAGGTTCTGCCATCACT
PRIMER_RIGHT_1_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_1=192,22
PRIMER_RIGHT_1=352,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.328
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=161
PRIMER_PAIR_PENALTY_2=0.6865
PRIMER_LEFT_2_PENALTY=0.014720
PRIMER_RIGHT_2_PENALTY=0.671790
PRIMER_LEFT_2_SEQUENCE=CGGACCGAAGGTATTGACTAAG
PRIMER_RIGHT_2_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_2=248,22
PRIMER_RIGHT_2=352,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=59.328
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.8061
PRIMER_LEFT_3_PENALTY=0.134318
PRIMER_RIGHT_3_PENALTY=0.671790
PRIMER_LEFT_3_SEQUENCE=AATGCTGGTGTCATTGTGAATC
PRIMER_RIGHT_3_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=352,22
PRIMER_LEFT_3_TM=59.866
PRIMER_RIGHT_3_TM=59.328
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.8827
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.671790
PRIMER_LEFT_4_SEQUENCE=AAGGTTTTAGCCTGCTGTCATC
PRIMER_RIGHT_4_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=352,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=59.328
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_00091 Ps_contig_mira-and-tgicl-ass_franssen_1156
SEQUENCE=TTGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAACTCCATAGAAGACTAAATTAATTGTTAATTACGTTCGGTTGGGGTAGGGACGGACCACGTACCTTAACTAAATTTAAACTTTAAAACTTTAAAACCTTAAAAACTTAAAAATTTAAATTTATTTTACTTTACGTTTTAGTTCGGTT
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1677
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.946688
PRIMER_LEFT_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT=76,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=60.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=1.3067
PRIMER_LEFT_1_PENALTY=0.360050
PRIMER_RIGHT_1_PENALTY=0.946688
PRIMER_LEFT_1_SEQUENCE=AACCTTGGTTCCTTGATAGCCT
PRIMER_RIGHT_1_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=60.947
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=1.4295
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=0.946688
PRIMER_LEFT_2_SEQUENCE=TCAACCTTGGTTCCTTGATAGC
PRIMER_RIGHT_2_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=60.947
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=1.4766
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=1.255615
PRIMER_LEFT_3_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_3_SEQUENCE=CCCAACCGAACGTAATTAACA
PRIMER_LEFT_3=76,22
PRIMER_RIGHT_3=185,21
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.744
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=1.5759
PRIMER_LEFT_4_PENALTY=0.629225
PRIMER_RIGHT_4_PENALTY=0.946688
PRIMER_LEFT_4_SEQUENCE=AACATCAAAGGTCTTTCCAACC
PRIMER_RIGHT_4_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=59.371
PRIMER_RIGHT_4_TM=60.947
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_00092 Ps_contig_mira-and-tgicl-ass_franssen_1157
SEQUENCE=TTGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGAACTCAGAGACAAGCAYGAATGAATGAATCMATGAAGCAATAATGTATATCTGGGTGGAGGCAGCTANAATATAATTACAATAAATAATNTTATCTTATGTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00093 Ps_contig_mira-and-tgicl-ass_franssen_1159
SEQUENCE=TTGTCCTTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAACTCCAATAGAAAGACTAAATTAAGTTGTTAATAGTCGGTGGGTAGGAACGAACCGACGTACCTTAACTAAAATTTAAACTTTAAAACTTTAAAACCTTAAAAACTTAAAATTAAATTATTTATTAC
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2874
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT=78,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.2904
PRIMER_LEFT_1_PENALTY=0.224014
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=TTCCTTGATAGCCTCTGCAAAT
PRIMER_RIGHT_1_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.224
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.3209
PRIMER_LEFT_2_PENALTY=0.254467
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=CTTGGTTCCTTGATAGCCTCTG
PRIMER_RIGHT_2_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.254
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=121
PRIMER_PAIR_PENALTY_3=0.4264
PRIMER_LEFT_3_PENALTY=0.360050
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=AACCTTGGTTCCTTGATAGCCT
PRIMER_RIGHT_3_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT_3=88,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.360
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.4702
PRIMER_LEFT_4_PENALTY=0.220992
PRIMER_RIGHT_4_PENALTY=0.249232
PRIMER_LEFT_4_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_4_SEQUENCE=CGTTCCTACCCACCGACTATTA
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_00094 Ps_contig_mira-and-tgicl-ass_franssen_1160
SEQUENCE=AGTAATTTAACCAACTATTTTATTGTAGTATAATGGCTATGATGGTGTAAGACACACATTATAATAGGATGAGGCAACATAAGATAATATTTATTTAGTTAGTAATTATATTGAGCTGCCTCCACCAGATATACATTATTGCTTCAGTTGGRTTCATTCATTCATGTCTTGTCTCTGAGCTATTTGCAGGNGGTCTATACGAAACGGGAAACCGTAAGGGTTGTAAA
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00095 Ps_contig_mira-and-tgicl-ass_franssen_1161
SEQUENCE=GATAATTWAACCAACATTTTATTGTAGTATAATGGCTATGATGGTGTAAGACACACATTATAATAGGATGAGGCAACAKAWRATATTTATTTAGTTAGTAATTATATTGAGCTGCCTCCACCAGATATACATTATTGCTTCATGGATTCATTCATTCATGCTTGTCTCTGAGCTATTTGCAGAGGCTATCAAGGAACCAGGTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00096 Ps_contig_mira-and-tgicl-ass_franssen_1189
SEQUENCE=TGGTATCAACGCAGAGTAGTCGGGKGACACAACACGTTAATTTTATCTTCACTCTTCTTCAGATCTCAAATTTCATTTCTCGCTCACATCGATTTCTCCTCCTCCTCCATGGCCGCTATTACTTCCATCAAGGCTCGTCAGATCTTCGACAGCCGTGGCAATCCAACCGTCGAGGTTGATATAACTGTCTCCGATGGCACTTTCGCTAGAGCCGCTGTTCCTAGCGGTGCATCCACTGGTATTTACGAGGCTCTTGAGTTGAGAGATGGAGGATCTGACTATCTTGGAAAAGGTGTATCAAAGGCTGTTGACAATGTGAACACCATCATTGCCCCAGCATTGATCGGCAAGGACCCAACTAAGCAGACTGAGATTGACAACTTCATGGTTCAGCAGCTCGATGGAACTGTTAATGAGTGGGGTTGGTGTAAACAGAAGCTTGGGGCAAATGCCATATTGGCAGTGTCTCTTGCAGTCTGCAAAGCTGGTGCTGGAGTCCTAAAAACCCCTCTTTACAAGCATATTGCAAACATTGCAGGCAACAAGAATTTGGTTTTGCCTGTTCCAGCTTTCAATGTCATTAATGGTGGATCACATGCAGGAAACAAGCTTGCCATGCAGGAGTTTATGGTTCTTCCTGTGGGAGCCTCCTCTTTCAAGGAAGCCATGAAGATGGGTGTGGAAGTCTATCACCATTTGAAGGCTGTGATTAAGAAGAAATATGGTCAAGATGCAATCAATGTTGGTGACGAAGGTGGTTTTGCCCCTAACATTCAGGAGAACAAAGAAGGTTTGGAGTTGCTAAAATCTGCCATTGACAAAGCCGGATACACTGGCAAAGTTGTCATTGGAATGGATGTTGCAGCTTCCGAGTTCTACAAGGAAGACAAGTCATATGATTTGAACTTCAAGGAAGATAACAACGACGGCTCACAGAAGATCTCAGGAGAGGCTTTGAAAGATCTCTACAAGTCATTTGTGTCAGAGTACCCAATTGTTTCAATTGAGGATCCTTTTGACCAGGATGACTGGGAGCACTATGCCAAGATGACTGGTGAGATTGGAACCAATGTGCAAATTGTTGGTGATGATCTCTTGGTTACCAACCCCAAGAGGGTTCAGAAGGCAATTGACTCAAAAGCATGCAATGCTCTTCTTCTCAAGGTGAACCAAATTGGATCAGTGACTGAAAGTATTGAAGCCGTGAGGATGTCCAAAAAGGCTGGATGGGGTGTCATGACCAGCCACCGAAGTGGAGAGACTGAGGATACCTTCATTGCTGATCTTGCTGTTGGTTTGTCAACGGGTCAAATTAAGACCGGAGCTCCATGCAGGTCAGAGCGTCTAGCTAAGTATAACCAGCTCTTGAGAATTGAGGAGGAGCTCGGTGCTGAAGCAGTGTATGCTGGAGTTAACTTCCGTACCTYCYGTGWYGACCCCTTAAAGTTAAAAAAGGGTTTTTAGGAAAAAAACGGGGGGGTAAAAAAAAACCCCCTAAAGGGTAAACGTTTAAACCGTTAAACCCCCCGTAAAAACCCGGTTTTAGGGTTTAAAGGGTTTTTTTTAACCGGG
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00097 Ps_contig_mira-and-tgicl-ass_franssen_1200
SEQUENCE=CGGGTTTTCCTAGTCCACCCCCTTCCCTTCCTTCCTTTTGTTCGTTAACCAACTGGTGGAGAGTCAGTGTGTTGAACGATGCTCTTAAGAGTATGTACAATGCTGAGAAAAGAGGAAAGCGCCAAGTCATGATAAGGCCATCATCCAAAGTTATCATCAAGTTCCTTATTGTTATGCAGAAGCATGGATATACTGGAGAATTTGAGTATGTTGATGATCACCGAGCTGGAAAGATTGTTGTTGAATTGAATGGTAGGTTAAACAAATGTGGGGTTATTAGTCCTCGTTTTGATGTTGGTGTCAAGGAGATTGAAGGTTGGACTGCCAGACTTCTCCCTTCTAGACAGTTTGGTTACATTGTTTTGACCACTTCTGCTGGCATTATGGATCACGAAGAGGCTAGGAGGAAGAATGTTGGTGGTAAGGTCCTTGGCTTCTTCTACTGAGAGTTTTTGTGATTTCAACCTGATGTAATGGAGGATCCTAGTTCCCTAGTAATTGAAGGAGCTGTTTTTGTGATGTGATTTAATTTTATTTTGTGAAACCTAGTTCAGTATAATTTATCTGATTTCTTCTATAAATTTTTGATTGTACCGGAATGAATGGCATACTTGTTTTGAATGGTATAGAATTTTATTATAATGTTGGAGACAYMAAGAGTTTCAATTGGTATGGTCCAGTTGTCAATATTTGGAAAGTATCAACTAGTTCTCAAGTTAGATTAAACAACTCTGAAAAATTTCATGTTTAAC
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00098 Ps_contig_mira-and-tgicl-ass_franssen_1206
SEQUENCE=GAATAATAATAATAATTGGAACACAAAAAACTACCATCACCACTAAGATTCCTCACATATCTATTCAGACCTCTTAGCATTGCCRWCRSSGAAATCACATACTAGAATGGCAACAACAACCTTTGCAACCGTCGTAGGATGGGTGGTGCATCACGTCTCACCTCAGGATTTGAACGAAACCACAAGTAACTGCTAGGAATTCCTTGAGGACAAGCTAGTGGGCGGTAAGGTAAAG
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00099 Ps_contig_mira-and-tgicl-ass_franssen_1210
SEQUENCE=GAAGTATTCAATTTTTTCATAGAAGTCAATAATAATAATAATAATAATTGGAACACAAAAACTACCATCACCACTAAGATTCCTCACATATCTATTCAGACCTCTTAGCATTGCCAACACCGAAATCACATACTAGAATGGCAACAACACTTGCACCGTGTAGGATGGGTGGTGCATCACGTCCTCCACCTCGAGGTAGTTTACGGGAAACCA
TARGET=28,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00100 Ps_contig_mira-and-tgicl-ass_franssen_1216
SEQUENCE=CCTACCTTACCCCTTCCCCGTTGGTTGGTCCGTTCCGTTCCGTACCTTACCCCTTCCCCGTTTGGTTCGGTCGGTTCGTTCCTACGACGTTCGTTAGTTAGGGTTCGGATAANAANACTCCTACTTAGTTTGTCNCCTCCTCTTTGTGAACCCAATGCAAAACATCCTAAGAGTGTAATTATCGGTGGTTTTCACATCAACATGGGCTTCAATAAGAGTTTGCCATTTGCGAACCAATGACCTGAGCTTGTCAGTTGTGAAATCCATACCCCAGAAGTTGGTCAAGAGGTTTTTCCCCTGAACATCTTCAGCCCTCAGTCTAATCTTCCTGAAAGCGTTGTCTTCATCTCCTTGAAGNTCAGCCAATGACTACCTACAAACACACGTATTGGTTTAGACGTCCAGTCGGGAANCAATTCGTTCGGGTACCCTGGA
TARGET=29,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4806
PRIMER_LEFT_PENALTY=3.425403
PRIMER_RIGHT_PENALTY=0.055230
PRIMER_LEFT_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_SEQUENCE=CGAACCCTAACTAACGAACGTC
PRIMER_LEFT=1,20
PRIMER_RIGHT=106,22
PRIMER_LEFT_TM=61.425
PRIMER_RIGHT_TM=60.055
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=3.5068
PRIMER_LEFT_1_PENALTY=3.425403
PRIMER_RIGHT_1_PENALTY=0.081398
PRIMER_LEFT_1_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_1_SEQUENCE=CACAAAGAGGAGGNGACAAACT
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=147,22
PRIMER_LEFT_1_TM=61.425
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=3.5349
PRIMER_LEFT_2_PENALTY=3.425403
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_2_SEQUENCE=CTGGGGTATGGATTTCACAACT
PRIMER_LEFT_2=1,20
PRIMER_RIGHT_2=273,22
PRIMER_LEFT_2_TM=61.425
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=3.5614
PRIMER_LEFT_3_PENALTY=3.425403
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_3_SEQUENCE=TCTTGACCAACTTCTGGGGTAT
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=286,22
PRIMER_LEFT_3_TM=61.425
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=6.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=3.5965
PRIMER_LEFT_4_PENALTY=3.541267
PRIMER_RIGHT_4_PENALTY=0.055230
PRIMER_LEFT_4_SEQUENCE=CTACCTTACCCCTTCCCCGTT
PRIMER_RIGHT_4_SEQUENCE=CGAACCCTAACTAACGAACGTC
PRIMER_LEFT_4=1,21
PRIMER_RIGHT_4=106,22
PRIMER_LEFT_4_TM=62.541
PRIMER_RIGHT_4_TM=60.055
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_00101 Ps_contig_mira-and-tgicl-ass_franssen_1217
SEQUENCE=TTGTTGGGGGGCGTAAGTTCGTTGCTAGTTTTTGGGCCTTATTGTATTGTTATTTATTTATTCTACAAGATTCTGAAATAGTTATCATATGAAGTATCGGTCCTATTTATGCATTGAGGTTGCAGACATTTGTTTATCCAATCTACAGTTTGTCCACAATTAGAAGTATTCCGTCAGCATTATTGAATTTATGACTTCATTAGTCTAGATGAATGGGATCAGCTGTTAATTCTGTAGTGGGGTAAGTTGGTGATTTTGATTGGGATCAGGGATTCATTTTTTAAGGGTCAGTTGAAAATAAAATTGTTCTTTTCGATTWCCACCGCTTTTTCTAAAGGGGCTTTGCATTGACTTTGTAAATTACTGCCGGAAAATGTGTTTTTTGTTGCATCATCAAACGGTTTTAAAACTT
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00102 Ps_contig_mira-and-tgicl-ass_franssen_1223
SEQUENCE=AAACTTTTTAAAATTTAAGTTACTTGCAACCTCAATGCATAAATAGGACCGATACTTCATATGATACATTTCAGAATCTTGTAGGATAAATAAATAACAATACAATAGGCCAAAACTGCACGAATTCGCCCCACAGACAACCCTATATTCCTCACGCAATATGGTAAAGAAACAAAAMSAGRRGGAATGACCGACGCGAAGCTCCAGCCAACAAATGGACCCTAAACCTTTAAACCCCCCCCGTTTTAAGGGACCTACCAAACTCCGGACCAACCC
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00103 Ps_contig_mira-and-tgicl-ass_franssen_1224
SEQUENCE=CAATTGTGGTTGGAAAGGGAACTTTCTGTAGAATGTGTATATTTAATAGAGTGTTCATGGTGTGATTGAGTCCTAAAGGGGGTTATGCATTTGTTGGCTAGGAGCTTCGCGTGTCATTCCTCTGTTTTGTTCTTACCATATTGCGTGAGAATATAGGGTTGTCTGTGGGGCAATTCGTGCAGTTTTGGCCTATTGTATTGTTATTTATTTATCCTACAAGATTCTGAAATGTAT
TARGET=201,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00104 Ps_contig_mira-and-tgicl-ass_franssen_1225
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTCAATTGTGATTGGAAAGGGAACTTTCTGTAGAATGTGTATATTTATGAGTGTTCATGGTGCGATTGAGTCCTAAAGGGGGTTATGCATTTGTGGCTAGGAGCTTCGCGTGTCATTCCTCTTGTTTTGTTCTTACCATATTGCGTGAGAATGTAGGGTTGTCTGKKGGGCAATTCGTGCTAGTTTTCGGCCTTATTGTATTGTTATTTATTTATCCTACAAGATTCTGAAAT
TARGET=225,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00105 Ps_contig_mira-and-tgicl-ass_franssen_1227
SEQUENCE=CYTAAGTTTGTTGTATTTGGTAAAGGGTAACTTTTCTTGTAGAATGTGTATATTTATGAGTGTTCATGGTGCGATTGAGTCCTAAAGGGGTTATGCATTTGTGGCTRGGAGCTTCGCGTGTCATTCCTCTTGTTTTGTTCTTACCATATTGCGTGAGAATAGTAGGGTTGTCTGGTGGGGCTAATTTCGGTGCAGTTTTGGCCTNTTGTATTTGTTTATTTATTTATCCTACAAGG
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00106 Ps_contig_mira-and-tgicl-ass_franssen_1253
SEQUENCE=GTTCGGGTCTTTCTTTCTTCCTAAGTCTACCCTTTCACTCCGAACAACCGATTCCAATGGCTTCAACTTCGATAATCTCAATGGCTGTGCCGGTAACCTGTGCCACCACCAAGAAGATTGAGTCACCGATCTCTCATGCATTCTTCAAGGCACCACNGGAKAKGTGAGCATCATCAAGTACTACGGAAGGTTTCAAGTTGAAACGCATCGCTGAAGGACGAAGTTGTGAACG
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00107 Ps_contig_mira-and-tgicl-ass_franssen_1262
SEQUENCE=TCAACAACAACAACAGCAGCCTCAACATCAACAGCAACAGCAGCCTCAACATCAACAACAGCAACAGCAGCCTCAACAGCAGCAGCTTCAACAACAGCTTCAACAACAGCAGCAGCAAGCAGCAGCTAGTTTATACAGTGGGGGACACTACATTCATCATAACCCTGCTAATTCCTGCATTACTATCCTAGTTATTACTCCTTCACA
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00108 Ps_contig_mira-and-tgicl-ass_franssen_1267
SEQUENCE=GTAGTAGGGTTTGGAGGGTTTTGGGGTCTACTAGAAGACATTGTTGTGGCAGAATCCCCTATATTGGCCTGCTGCACAGGAAGATTGTAAGCTTGTGGCTGTCTTGGAACATAGTAAACCTGATGATGGGGCTGTTGCTGTGAAGGATATACAGGATAGTATGCAGGAATTGCAGGGTTATGATGAATGTAGTCGTCCCCCATAGTATAAACTGCTGCTGCTGCTGCTGCTGCTGTTGTTGAAGCTGTTGTTGAGCTGCTGCTGTTGAGGCTGCTTGTGCTTGTTGTTGTAGTTTGGGTTTAGGGACGGGTCCTTGGTCCGTTTGGTTTCGGTCGTTTGGTTGTAGTTTGGGTTAGGGACGGGTTCCGTTGGTTCGTTTTGGTTTTTGTTTTTACGTTTTTAACCAAAAC
TARGET=212,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2790
PRIMER_LEFT_PENALTY=0.143555
PRIMER_RIGHT_PENALTY=0.135429
PRIMER_LEFT_SEQUENCE=TAGTAAACCTGATGATGGGGCT
PRIMER_RIGHT_SEQUENCE=CCCTAACCCAAACTACAACCAA
PRIMER_LEFT=111,22
PRIMER_RIGHT=357,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=60.135
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.3035
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.135429
PRIMER_LEFT_1_SEQUENCE=CTTGTGGCTGTCTTGGAACATA
PRIMER_RIGHT_1_SEQUENCE=CCCTAACCCAAACTACAACCAA
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=357,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=60.135
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.3286
PRIMER_LEFT_2_PENALTY=0.143555
PRIMER_RIGHT_2_PENALTY=0.185055
PRIMER_LEFT_2_SEQUENCE=TAGTAAACCTGATGATGGGGCT
PRIMER_RIGHT_2_SEQUENCE=ACCCAAACTACAACCAAACGAC
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=352,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.3449
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=0.201297
PRIMER_LEFT_3_SEQUENCE=TAGTAAACCTGATGATGGGGCT
PRIMER_RIGHT_3_SEQUENCE=CGTCCCTAACCCAAACTACAAC
PRIMER_LEFT_3=111,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=59.799
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.3463
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.135429
PRIMER_LEFT_4_SEQUENCE=AGGAAGATTGTAAGCTTGTGGC
PRIMER_RIGHT_4_SEQUENCE=CCCTAACCCAAACTACAACCAA
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=357,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.135
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_00109 Ps_contig_mira-and-tgicl-ass_franssen_1268
SEQUENCE=TTTGAACTCCAGCAGCAACAGCAGTCAATTCGAACTACAGCAACATCAGCAACATCAGCAACAGTTTGAACTCCAGCAGCAACAGCAGCCTCAACAACAACAACAGCAGCCTCAACATACAAACAGCAACAGCAGCCTCAACATACAAACAACAGCAACAGCACGCCTACAACAGCAGCAGCTACAACAACAGCCTTACAACGACA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4072
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.187326
PRIMER_LEFT_SEQUENCE=CATCAGCAACAGTTTGAACTCC
PRIMER_RIGHT_SEQUENCE=TTGTTTGTATGTTGAGGCTGCT
PRIMER_LEFT=52,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.4771
PRIMER_LEFT_1_PENALTY=0.219884
PRIMER_RIGHT_1_PENALTY=0.257221
PRIMER_LEFT_1_SEQUENCE=ATCAGCAACAGTTTGAACTCCA
PRIMER_RIGHT_1_SEQUENCE=TGTTGTAGCTGCTGCTGTTGTA
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=188,22
PRIMER_LEFT_1_TM=59.780
PRIMER_RIGHT_1_TM=59.743
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.5377
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.317786
PRIMER_LEFT_2_SEQUENCE=CATCAGCAACAGTTTGAACTCC
PRIMER_RIGHT_2_SEQUENCE=GCTGTTGTTTGTATGTTGAGGC
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=155,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=59.682
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.5377
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.317786
PRIMER_LEFT_3_SEQUENCE=ATCAGCAACAGTTTGAACTCCA
PRIMER_RIGHT_3_SEQUENCE=GCTGTTGTTTGTATGTTGAGGC
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=59.682
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=0.5377
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.317786
PRIMER_LEFT_4_SEQUENCE=CATCAGCAACAGTTTGAACTCC
PRIMER_RIGHT_4_SEQUENCE=GTTGTTTGTATGTTGAGGCTGC
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=59.682
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00110 Ps_contig_mira-and-tgicl-ass_franssen_1291
SEQUENCE=CMAAAAACACAGCAATATTTCATTCCTCAATTTCACATCCCTGCACAAATTCAATAACTACACCAAAGGCATAATTCGATATATTTGAATACATAAACCCTAATCAATCACACCAATGTTAATTTATTGAATGATTCAAACGATAAGTATAACATCATTTTACATTACCTGAAGCATTAAATTAACACAAAACCCCTTTCTAGAAAGTAAAAGTTCTAATTATTATCGAAACAAAGGGGATTCAACAACATATTCATACAAACACACTCCAGAATAAGATGATTCATGATAGAGATTATAGAGTTGAATTAATTAATTAACTAGGGTTGCAACAACAGGGTACTTAGCGACGAACTTGGTTTCCCAATCATTGAGAACTCCGATCTCTTTCTCGGTGAGCCCATCCAGTGACGAGGTGATGTCTTCTTCGTTTTTGCTCATCTTCGCCAGTGCTCTGCTCGCGTCTCTGCCGGCGAACATAGCGTAGGCTCCGCCGGGACCGTAGAACGATTTGCCGGTGGTGACGTCGAACACGCGACCCTTCACTGCAACGTATATGGGCTTCGATGGATCTGCGCCGTTGTATGCGCTGAGTTGCTGCGTTGTAATCTCCATTTTTCTTCTTGAATCTCACACCGCACAAAGAAGCAAGGTTTCGCTATGGTTTCACCTTTCTATCCCCCGAGTACT
TARGET=307,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00111 Ps_contig_mira-and-tgicl-ass_franssen_1292
SEQUENCE=AAGCAGGTGTTCAACGCAGAGACGTCGAGCAGAGCACTGGCGAAGATGAGCAAAAACGAAGAAGACATCACCTCGTCACTGGATGGGCTCACCGAGAAAGAGATCGGAGTTCTCAATGATTGGGAAACCAAGTTCGTCGCTAAGTACCCTGTTGTTGCAACCCTAGTTAATTAATTAATTCAACTCTATAATCTCTATCATGAATCATCTTATTCTGGAGTGTGTTTGTATGAATATGTTGTTGTAATCCCCTTTGTTTCGATAATAATTAGAACTTTTACTTCTGAAAGGGTTTAGGTTGGTTAATTTAAATTAGTCTTCTAGGTAAGTAGTAAAATTAAGATGTTATTACTTAATTCGTTTGTAAGTACTATTACTAAACTAAAATTAAAACTAAACTTGGTT
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3915
PRIMER_LEFT_PENALTY=0.174657
PRIMER_RIGHT_PENALTY=0.216800
PRIMER_LEFT_SEQUENCE=GAAACCAAGTTCGTCGCTAAGT
PRIMER_RIGHT_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT=123,22
PRIMER_RIGHT=261,22
PRIMER_LEFT_TM=59.825
PRIMER_RIGHT_TM=60.217
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=0.4378
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.216800
PRIMER_LEFT_1_SEQUENCE=CTCAATGATTGGGAAACCAAGT
PRIMER_RIGHT_1_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_1=111,22
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.217
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.5000
PRIMER_LEFT_2_PENALTY=0.283237
PRIMER_RIGHT_2_PENALTY=0.216800
PRIMER_LEFT_2_SEQUENCE=ACCAAGTTCGTCGCTAAGTACC
PRIMER_RIGHT_2_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=261,22
PRIMER_LEFT_2_TM=59.717
PRIMER_RIGHT_2_TM=60.217
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.7899
PRIMER_LEFT_3_PENALTY=0.573098
PRIMER_RIGHT_3_PENALTY=0.216800
PRIMER_LEFT_3_SEQUENCE=CAATGATTGGGAAACCAAGTTC
PRIMER_RIGHT_3_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.573
PRIMER_RIGHT_3_TM=60.217
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.8415
PRIMER_LEFT_4_PENALTY=0.624727
PRIMER_RIGHT_4_PENALTY=0.216800
PRIMER_LEFT_4_SEQUENCE=AGAAGACATCACCTCGTCACTG
PRIMER_RIGHT_4_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=59.375
PRIMER_RIGHT_4_TM=60.217
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_00112 Ps_contig_mira-and-tgicl-ass_franssen_1312
SEQUENCE=TGGTACATATCAGATGAAGCTCAAAAGGAGCGAGTCAGTCAGATGATTGAAGATTTTGAACCTTTTGAGGGAGAGGCTCTTTTGGATGCCAAGTGCTTCAAGTGATAATTTATTATATGCAAGGTTATTATGCTCGTCTTTGTTTTTGGTATAGTTTAGTTTCTTTGTTATCTCAGTTTCAGGGAAGTTCAAGCCGATACTTCGATTTGACTTGATGTTTTCACCATAGTTTTCATTTTTGCAAGGGAAGACTAGTATCTTTTTTTAGTATGTATGAAAAATTGTGGATTCAGTGTGTTTTTTGGTCCCCTGTTTTGGTACATGGGTTCGAAGTGTTTTTTATTTGTTTTAAAAAATAAAGAAATTTGATGGAACACTTACAAAGGAGGGGAGCATGCTTGAATTCGAAATGTTGATTTATTTATTTATATTTTTATCAY
TARGET=416,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00113 Ps_contig_mira-and-tgicl-ass_franssen_1328
SEQUENCE=ATGGTACCAGAATACTAGAGANTNTATNCGGATCGTTCTGGATNTTCCCGTGTTTTATAACTACTGATCTTCTGGCTCGTGGTATTGATGTGCAGCAAGTGTCTTTAGTTATAAATTATGATCTCCCCACACAACCCGAAAACTATCTCCATCGTATTGGTCGTAGTGGAAGGTTTGGTAGGAAAGGTGTTGCAATTAACTTTGTCACGAAGGATGATGAAAGAATGCTGGGTGACATCCAGAAGTTCTACAATGTGTTGATTGAGGAGCTTCCTTCCAATGTGGCTGAACTTCTGTGATGAAATTTTTCTTCCATCATACTAGARGGAAGTTGGTTTCTGAGTAAAAAATGGTTCAATTTTGGCCACTTTATTTTCAGTTGTGAACTTTCTAATTGTGGTATTGTCAAGTAGCCGGGGCATGGCGTTGCAATTCAGAAATTTGAATACAACTTGTAATCATYCTGCAATTCTGCACTATTCAATCAATCAATTAGACGTTGTGGCATTATAAAACTTGAAGCAGAATTTAAAAAAAACCACTTATGTAATGACATTATATTACTGATCGTTTAATCTTCTACTAGTGGTGAATTT
TARGET=481,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00114 Ps_contig_mira-and-tgicl-ass_franssen_1331
SEQUENCE=AAACCAAAGGTTTACGTAAAACAAGAAAATAACAAACAAACAAGGGCGAAAAGGTAAGGGAAACGAATTAACATATAACTTAACCTTCCTAGAAACCAAACTTACCTTCTAGTATGATGGAGAAAAATTTACATCACAGAAGTTCAGCCACATTGGAAGGAAGCTCCTCAATCAACACATTGTAGAACTTCTGGATGTCACCCAGCATTCTTTCATCATCCTTCGTGACAAAGTTAATTGCAACACCTTTCCTACCAAACCTTCCACTACGACCACGATGGAGATAGTTTTCGGGTTGTGTGGGGAGGATCATAATTTATAACTAAAGACACTTGCTGCACATCAATACCACGAGCCAGAAGATCAGTAGTTATTAAAACASGGAAGATCCASAACGGAATTCCCGCATAATAATATCTCTAGTATTCTGGTCCATGTCTCCGTGTGTTGCTGAGACTGTATGGTCACGGCTTCGCATCTTGTCAGTTAGCCAATCAAACTTTACCGTACTAGGTAGTTACAACAAAAAATAGAAC
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00115 Ps_contig_mira-and-tgicl-ass_franssen_1342
SEQUENCE=CCCCGGTTTTTCGGTTCCCGGTTCGGGTTCCGTTCCCTACGGGGAAAACCGTTTTACCCCTAACTTGGTTTGGTTCGTTCCCAAACCTACCCGTTTACCTAACGTGCACCCTCCAAAACTAAGGACCCAAAACTAGGAACAATCATGCGCCTGTTGCACAGTCTCAAACACCAGGTGTTTCTTGTCTGGTGATAGAACAACATGTTGCCACTCTGTCATGGCTATGTCAGGAGGGCACATAGGGCTGTTACAAAGGGATCTGAAGGTTATCTCTCGGACTTGACCATCATATTTTTCTGCTGTATGCCACTGTCCCATCACAAGATTAGTATCCTTTCGTACTGAGCGATACATGCTAGTAAACTTTGAATCATCCTTTAACAATAAATTAGAAAAACTGCTCAGCTGTGCAGGGGAAAACATCATTGTATATACCAACTAAAGTCTTTCTTTCTTTGATTAGTTAAAAGTTTTAAGTTATACTAACGTTTCGG
TARGET=445,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00116 Ps_contig_mira-and-tgicl-ass_franssen_1353
SEQUENCE=GGKTTTGTAAGGAGTGGGTTACTGCGGTGGTGAAGCTTGAGGTGAACGAAGAAGCGGGTGGTGCTGATGTTCATTTTGAAGCTTTTCAGATGAGTGATGTGTGTATTAGGTTATTTAAGGAGGTTGGTTTGACACTGGAAGGGTTAAGGAGGATCATGATCCTTAAGTTGTCTAATAATGAAGAGNTGTGTGTGTGGTGTAAGGATACTAAGNGTTNATAATGTTATTTTTTCGTGGGTGGTTGTAA
TARGET=187,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00117 Ps_contig_mira-and-tgicl-ass_franssen_1379
SEQUENCE=ATCTTCTTCTTCTTCTTTATAACCCAATCAACAGAGAAAAAGAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATTGGTGATTCATAGATTAGAATTATGCATCTCTAGTGGTTAGAATGGCCATAGAGTTTGTATGTCAGTTGCAAGTAACCGTTGTTATTGTTACAAAGGAAAAGAAACACACGTACCCTTTTTGTTCCGACACCGTNCAATCTCTCTTCTTTNACGTT
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00118 Ps_contig_mira-and-tgicl-ass_franssen_1380
SEQUENCE=AGTACGCGGGGACTCYTCTTCTTCTTCTTCTTCTTCTTATAACCCAATCAACAGAAGAAAAAAGAAAGCAGTTTCTAGTTTGAGATTCAGATCGGAAGAAAGTGATGGTGATTCATAGAATTGAACTATGCATATCTATGGTTAGAATGGCCATAGTAGTTTGTTATGGCAGTTGCAAGGTAAACGGGTTTGTTTATTGTTACAAAGAAAAGAAACCACCCGTTACCCTTTTTGTTTCCGTTCAGCCGTGACAAAATTCCTTCCCTTCCTTTCCTTTTCACGGTTATTCTTTCGTTG
TARGET=17,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00119 Ps_contig_mira-and-tgicl-ass_franssen_1381
SEQUENCE=TTTTAAAAATTRACCTGAATCAATTCATTCATTCATTCATAATCAACATAACTTGTGCATCATAATCTTAGGAAATTAACCAAGTAGTTTAGAAATAAAAACCAAACCCTAAAAGCATGGTTGTNTCTAATTTTCCTTANATTGTTGGCATACAAATAACAACACGATGACCAACCAAACCGAAAAAGGGTAAATTNTGTACTGATACTAAATATATATACAGTAGTTTTANAGAAAC
TARGET=23,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00120 Ps_contig_mira-and-tgicl-ass_franssen_1383
SEQUENCE=GGWTAGAAATACCTGAATCAATTCATTCATTCATTCATAATCAACATAACTTGTGCATCATAATCTTGGAAATTAACCAAGAGTTTGAAATAAAAAACCAAAACCCTAAAAGCATGGTTGTATCTAATTTTCCTTTACATAGTTTGGCATCAAATACAACACGATGCACAGACAAAAAGGGAAATTNTGTACTGATCTAAACTATATATACAGTAGTTTTANAGAAACAAGAAATTAACAACAACAAACTAAG
TARGET=21,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00121 Ps_contig_mira-and-tgicl-ass_franssen_1384
SEQUENCE=CTCTTCTTCTTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCGGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGAATTGAACTATGCATATCTATGGTTAAGAATGGACCAATAGTAGTTTGTTATGGCTAGTTGCAAGGTAAACGGTTTNTTTATTGTTACAAAGGAAAGAACCGCCGTACCTTTTGTCCTNACGCCGTGACAAATCCTCCTCTTCCTTT
TARGET=2,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00122 Ps_contig_mira-and-tgicl-ass_franssen_1385
SEQUENCE=ATCTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGATTAGAATTATGCATCTCTATTGGTTAAGAAGTGGCCATAGAGTTTGTTATGTCAGTTGCACGAAACCGGTTTGTTTATTGTTCAAGAAAAGAAACACAGTACCCTTTTTGTTCCGTACAACCGTGCAAATACTCCTCTTCTTTCACGTTA
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00123 Ps_contig_mira-and-tgicl-ass_franssen_1386
SEQUENCE=TTTAGAATGGTCCATAGAGTTGTTTATTGTCAGTTGTCAGAAACCGTTACGGTTTATTTAGTTTCTAACGAAARAAAACAACAGACCCTTTTAGTTTCCGTTRCTAACCGTAGCAAATAMTCCTCTTCCTTTTCACGGTTACCTTCGTTGATGATTTCTATCTAGCATGTTTTTTCTTCAGTTTGTGTTGTTAATTTCTGTTTCATAAAACTCTGTATATATAGTTTAGATCAGTACATAATTTTCCCTTTTGCTGTGCATCGTGTTGTATTTGATGCCAAACTATGTAAAGGAAAATTGATACAACCATGCTTTTAGGGTTTGTTTTTATTCAAACTCTTGGTTAATTTCCAAGATTATGATGCACAAGTTATGTTGATTATGAATGAATGAATGAATTGATTCAGGTATTTCTATTTCTTCAAYTTCTTCTTGTACTGTTTGCTGGTTCACTTTGCTTAATTTTCTTCTTCTTGTTTGTTAATTCTTTCATTGAAATTGGGAAAAATAAATTCTGTATTTTCTTTAG
TARGET=382,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00124 Ps_contig_mira-and-tgicl-ass_franssen_1387
SEQUENCE=CCGTGAAAGGAAGAGGAGATTTGCACGGTTGTACGGAACAAAAGGGTCTGTGTTTCTTTCTTGAACAATAACCACGGTTTCTGCAACTGACATAACAAACTCTATGGCCATTCTAACCATAGAGATGCATAATTCTAATCTATGAATCACCATCACTTTCTCCGATCTGAATCTCAAACTGAAACTGCTTTCTTTTTCTCTGTTGTTGGTTATAAGAAGAAGAAGAGATAGTACTCTGTCGTTGAACTACCCACTGTCCTTTCGTAGGAACGACCACCGACAAACGGGAGGGGTA
TARGET=214,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1137
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.047267
PRIMER_LEFT_SEQUENCE=AATAACCACGGTTTCTGCAACT
PRIMER_RIGHT_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT=66,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=60.047
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.1617
PRIMER_LEFT_1_PENALTY=0.114470
PRIMER_RIGHT_1_PENALTY=0.047267
PRIMER_LEFT_1_SEQUENCE=GGAACAAAAGGGTCTGTGTTTC
PRIMER_RIGHT_1_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=60.047
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.2145
PRIMER_LEFT_2_PENALTY=0.167242
PRIMER_RIGHT_2_PENALTY=0.047267
PRIMER_LEFT_2_SEQUENCE=AATCACCATCACTTTCTCCGAT
PRIMER_RIGHT_2_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_2=144,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=60.047
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.8000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.2813
PRIMER_LEFT_3_PENALTY=0.234069
PRIMER_RIGHT_3_PENALTY=0.047267
PRIMER_LEFT_3_SEQUENCE=CGTGAAAGGAAGAGGAGATTTG
PRIMER_RIGHT_3_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=60.234
PRIMER_RIGHT_3_TM=60.047
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.3706
PRIMER_LEFT_4_PENALTY=0.323355
PRIMER_RIGHT_4_PENALTY=0.047267
PRIMER_LEFT_4_SEQUENCE=CGGTTTCTGCAACTGACATAAC
PRIMER_RIGHT_4_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=60.047
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_00125 Ps_contig_mira-and-tgicl-ass_franssen_1388
SEQUENCE=AAAAACATGCTAGATAGAAATCATCTTAACGTAAGGTTAACCGTTGAAAAGGAAGAGGAGTATTTGCACGGTTGTACGGAACGAAAGGGTCTGTGTTTCTTTCTTGAACAATAACAACGGTTTCTGCAACTGACATAACAAACTCTATGGCCATTCTAACCATAGAGATGCRTAATTCTAATCTATGAATCACCATCACTTTCTCCGATCTGAATCTCARACTGAAACTGTCTTTTCTTTTCTCTGTTGATWGKTTAGRRGWKAAKARAGGAAAGGAAAGGAAAGAGTATTAGTTACTCGTGGCGTTTAGACTACC
TARGET=269,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00126 Ps_contig_mira-and-tgicl-ass_franssen_1389
SEQUENCE=ATCTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTYGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGATTAGAATTATGCATCTNTATGGTTANATGGCATAGTAGTTTTGTTATGTCAGTTGACAAGTAACCGTTTGTTATTGTACAAAGAAAGAACNCACGTTACCCCTTTTTGTTACCCGAACAACCGTGAACCTAAAAATTACCCTTTCCCCCTTTCCTTTTTACCCCGTTTTTTACCGAACCGGGGTTTTTAA
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00127 Ps_contig_mira-and-tgicl-ass_franssen_1395
SEQUENCE=CAGTTAAAAGATGCTGAAGAAGAGTTGGAGAAAGTCAAAAGAGGGGAAGCAAAAAGGCTTGATTTAGATTCATTGCTGTGAATCATATGAATAATGCCGTTATTGTAATTTCCTTTTGTCACTATTTTTGTTGTTGTTGAAGTTTTACATTGTTATACGAACTTCTTTANGTGTTGGATTCAAATGTAAGTCATTTATCTAGTTTTGTTCTTATTTTAATCAATAAAAAGT
TARGET=128,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00128 Ps_contig_mira-and-tgicl-ass_franssen_1406
SEQUENCE=TTCGGGGTAGAGCATTTGAAGTGTTTACGGCTGCACTCTCTCTCTCGRCTCAATAWGGCTGAACCGTCGTTNCTCGTCCCCGTCGCCGTCGAAGTCGATCCCAATCAATCGAAGTCAAGCTCTTCAACNGAGT
TARGET=36,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00129 Ps_contig_mira-and-tgicl-ass_franssen_1448
SEQUENCE=TGGAAGAGGAAGTTGAAGAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATTATTTGACTAAAATTAATTTTTTAGAAGACCTACCCTTTTATTTTATCTGTTGTAGTAAACTAGAGTCACCTATYTGTGTATATTTATTTATTATTATGAACTCTAGTGTTATTTTGTTATGTGTWMTYTATGAGTCGGGACCGGACCGTA
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00130 Ps_contig_mira-and-tgicl-ass_franssen_1449
SEQUENCE=TGGTAAGTAGGAAGTTGAAGAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATATTGACTAAATTATTTTTAGAAGACCTACCTTTTATTTATCTGTTGTAGTAAACTAGAAGTCTACCTTATTTGTRTATTATTATTATTATTA
TARGET=176,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00131 Ps_contig_mira-and-tgicl-ass_franssen_1450
SEQUENCE=TGGTAAGTAGGWAAGTTTGTAAGWAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATATTTGCTAAATATTTTTAGAGACACCCTTTATTTTATCTGTTGTGTAAACTAGAAGTCACCTATTTGTGTATATTATTATTATTATGACTCTAGTGTATTTTGTATGTGTATCATGATGGACGCCGACTATAGGAACRACGTTAGTCGAAC
TARGET=176,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00132 Ps_contig_mira-and-tgicl-ass_franssen_1451
SEQUENCE=GTGGTATCAACGCAGAGTTGGAAGAGGAAGTGAAGAAGATGAGTACGATAAGAGGGTCGAAAGAAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATATTTGCTAAAATAATTTTTTAGAAGACACCYTTTATTTTATCTGTTGTGTAAACTAGAAGTCACCTATTTGTGTATATTATTATTATGACTCTAGTGATTTGTATGTGTATCATGATGGACGCCGATATGAGAACAATTG
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00133 Ps_contig_mira-and-tgicl-ass_franssen_1452
SEQUENCE=CCTGGCATGTAATCAGAAGATTACKGTTCTCAAACATGGCAATTTAGAATGTAATGAGACTAACATTGTTTCTCATAGTCGGCGTCCATCATGATACACATACAAAATCACTAGAGTCATAATAATAATAATATACACAATAGGTGACTTCTAGTTTACACAACAGATAAATAAGGGTGTCTTACTAAAGWATTWATTTTARRCAAATATGSAMGGAGTTTGGGTGCTTAACTGATATGGAATTCTCC
TARGET=119,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00134 Ps_contig_mira-and-tgicl-ass_franssen_1459
SEQUENCE=TACCGTAAGTCAACTGATCGAACTTGTTTTTTCCTTTTTTTAATTTTTAAAACTTAAAAAATTGAAATTATTATTATTAGGTTCTTTCTTGGTTAGCTTTAAAAATTGTAATGTTTTGTTATGCATGTATTGTATTGCAGTTGTTTCTGGATGACAGCGTAAGAAACA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4138
PRIMER_LEFT_PENALTY=1.296517
PRIMER_RIGHT_PENALTY=0.117281
PRIMER_LEFT_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_SEQUENCE=TTCTTACGCTGTCATCCAGAAA
PRIMER_LEFT=2,23
PRIMER_RIGHT=164,22
PRIMER_LEFT_TM=59.703
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=1.4138
PRIMER_LEFT_1_PENALTY=1.296517
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_1_SEQUENCE=TTTCTTACGCTGTCATCCAGAA
PRIMER_LEFT_1=2,23
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=59.703
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=1.4722
PRIMER_LEFT_2_PENALTY=1.296517
PRIMER_RIGHT_2_PENALTY=0.175676
PRIMER_LEFT_2_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_2_SEQUENCE=TACGCTGTCATCCAGAAACAAC
PRIMER_LEFT_2=2,23
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=59.703
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=1.6014
PRIMER_LEFT_3_PENALTY=1.296517
PRIMER_RIGHT_3_PENALTY=0.304841
PRIMER_LEFT_3_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_3_SEQUENCE=CTTACGCTGTCATCCAGAAACA
PRIMER_LEFT_3=2,23
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=59.703
PRIMER_RIGHT_3_TM=60.305
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=1.6014
PRIMER_LEFT_4_PENALTY=1.296517
PRIMER_RIGHT_4_PENALTY=0.304841
PRIMER_LEFT_4_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_4_SEQUENCE=TGTTTCTTACGCTGTCATCCAG
PRIMER_LEFT_4=2,23
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=59.703
PRIMER_RIGHT_4_TM=60.305
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_00135 Ps_contig_mira-and-tgicl-ass_franssen_1460
SEQUENCE=TATGCATGTATGTATTGTATTGTCTAGTTGTTTCTGGATGACAGCGTAAGAAACATTGCTGCGGGTAAAGAAATGGGTCTTCACACTGTTTTGGTAATTTTACAAACCWAAAGTCTTATGATGTTGATTTATTTATTTGTGATTATGAAATTAATTAATGATGTTTGTTTAGGTTGGGGAGGGGGTGGTAAAAGACAAAGTTGGGGATTATTGTAGTGGATGTGTGAATAGAGTTGCACTTGCGGAAGTG
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00136 Ps_contig_mira-and-tgicl-ass_franssen_1485
SEQUENCE=TCCAGTGGTCGTCCCAAGCATGGTCTCTGTTGTATATTTTGGCACCTCTTCCAATTGGGTTTGTTGTGTTTTTGGTTCACTTGGCTACTATTCCTATAACTGGAACTGGTATTAACCCTGCTAGGAGTCTTGGTGCTGCCATAATATAMACAAAACAAAGAAAGAACCAATAGCTTGGGACGACCACTGGATATTCTGGGTTGGACCTTTTATTGGAGCTGCCCTTGCTGCACTATATCACCAGATCGTGATTCGAGCAATTCCTTTCAAGACAAGGGGCTAATGAATCCATCATCATCATTGACATGTCTTSKTGTTTWGAATTATCTATTCGGTGTATTCATTTCTTTGTTTTATTTTTTTATCTTATGTTAGTAAATTATGAGCTCGTGTTGTATGGACTGGTGGAGGTGGAGTATTTCTTTCTTAAAACGG
TARGET=290,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00137 Ps_contig_mira-and-tgicl-ass_franssen_1494
SEQUENCE=CCTAGTTTTAGATCTCTTTATTGATTATTCATGTGATTGGTTGTTGCTGAAATTTCAAGTTATTTAAGTTTTACTTTACTTTACTTTTATCTAGATTGTTACTCATTATTTTAATTTAAAATGAAAATTAGTAAAAATTTAAAATTTAAAAAAAAMCCTTTT
TARGET=70,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00138 Ps_contig_mira-and-tgicl-ass_franssen_1535
SEQUENCE=ACCCCGTTTTAACCCTTAACCTAAACCTTTTACCTTAAACCTAAACGAAGTTTAACTAACTAACCAAAAATTTAAAACTAAAAGTTTAACGGTACCGAAAAACCTTTAACGTTAAAGGCTTTAACTCTAANCAACAACACAAAGAACCAAGTAAACTAGTACATCACTTGTACAACTGAAAATAACAATAAAATAACAAGTCAATTGTCTGGCCTCAATCTCTTGCTAAATCGATGCAGGGACAAGAGGCCGACCTCGTCCGATTGCAAAACCCCGTGTTCCATCAGGCATTCTTGGCCCAGGAAGGCGGTTTTGATGCTTCAGCGGCWATRAGGAATAAATGATGTACCTCCATGGCCCATTCCATTTGTAGCACGATATTTGTGCTTTCNCGATCTGCCTGTTTAGGGTACCTCTGCCCCACCTTTTATCCTTTGACAAATGCCTCCCCTATTCCCTTTTTCCTCAAAGAAATTATTCCTTCATGATGCTCATGTGAGTCGTGA
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00139 Ps_contig_mira-and-tgicl-ass_franssen_1623
SEQUENCE=AACTGTTCCCGGAGGACCAACGGTTGCATGTAGTCACAGCAAAAGCTGTTTGAGTGGGATGCTGCTTAAAGGTCAAACAACCTCGATCCTTCTGGTAGAGCAGCACTCGGAGTTTCATGATTCAGCTTATGAAAACCATAACAAAGTCAACAAAACTGTAGAGTTTGAGAAGATTATACCACTGGAAGCCGCTCCTGTCGAGCTTGCCATCCAGGGCTAGTTTCNGCTATGGCAAGGATGACTGTGCTAGAAGAATGAAGATAATATGAAACTTANATAATGAAGTAATTTGCCCTTTCGTTTAGGTTTTTTAAAATTTTTACGGCCCGGGGGGACAAAAACTAATTGGACCTTAATTAATTAAATTAAG
TARGET=353,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00140 Ps_contig_mira-and-tgicl-ass_franssen_1625
SEQUENCE=AGTTTCCAGAACTCTCAATACATAATAGCAGGGAATGCGAGAATATTAAATCAATATMTACACAACCACAATTATAGGCTCTATTTTGATTGACTAAAGTTATAACAAAATATTATACAAATAACTGTTGACCAAATTAATACTGAGGCTGCTTCCTTTTCCCTTGTATTTATTTTTTTGAAAGAAAGAAAAAAAGGACTAGACAAAGTAAAGGATGCCATTCTGCAAGGCATGAGTAGATTCTCTATGCTGCTTCGACAAAACCACTCTGCGTTGACTACCCACTGTCCTTC
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00141 Ps_contig_mira-and-tgicl-ass_franssen_1627
SEQUENCE=TGGATTTGCCGAAGCAGCATAGAGAATCTGCTCATGCCTTGCAGAATGGCATCCTTTACTTAGTTCCTTTTTTTCCTCTTACAAAAAAATNTCAAAGGGAAAGAGCAGCCTCAGTTATTATTTGGCAACAGTTTATTTGAATTAATTATTTTGTAATTAACTTTGCAATACAAAATGAGCCTATATGTGTTTGTATATATATATWKTRATTTTAATATTCTCGCTACTTTCCCTGTA
TARGET=194,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00142 Ps_contig_mira-and-tgicl-ass_franssen_1634
SEQUENCE=AAAAAAACGTTTTTTTAAACCCTTTTTTTACCGGGGGGGTTTTTCGGGGGGTTCCGGAACCGGTTACCCGTAAACCTTTACCTAAAACGGGTACCCGAACTTTAAGGGTTTAGGGTAACGGCCGTGTTTTTGGGTTAGTTGGTTAAGTTAGATTTTGTATATTGTAAGACTGGTGGTCAACATTTTGTATGAACTTGCAAGAGCACTAAATGGTTTTAGTTGCACAAAAGAAGAGTTTACTTGTCTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTTGGGTTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGACTAAAACAAGTGTTCTTAGGTGTTCCTGCTAATGCTGCTGCTGCCTACCAAGGTGGTGTCTTTATTCCTTNTGTATTTGGTTTTTGTACTTTTCCTACTAACGTTTCCTTCCTGACTTTAAGTTTAAAGGTACGTTTTCCCTTAACCCTTAAAAAAGTTTACGGTTT
TARGET=448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3386
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.335045
PRIMER_LEFT_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_SEQUENCE=AAGGGTTAAGGGAAAACGTACC
PRIMER_LEFT=379,22
PRIMER_RIGHT=564,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.665
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.3394
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.335045
PRIMER_LEFT_1_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_1_SEQUENCE=AAGGGTTAAGGGAAAACGTACC
PRIMER_LEFT_1=383,22
PRIMER_RIGHT_1=564,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.665
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.6148
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.611210
PRIMER_LEFT_2_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_2_SEQUENCE=AGGAATAAAGACACCACCTTGG
PRIMER_LEFT_2=379,22
PRIMER_RIGHT_2=483,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.389
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.6156
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.611210
PRIMER_LEFT_3_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_3_SEQUENCE=AGGAATAAAGACACCACCTTGG
PRIMER_LEFT_3=383,22
PRIMER_RIGHT_3=483,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=59.389
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=1.0113
PRIMER_LEFT_4_PENALTY=0.676221
PRIMER_RIGHT_4_PENALTY=0.335045
PRIMER_LEFT_4_SEQUENCE=GATGCTCATTTGGATTCAGCTA
PRIMER_RIGHT_4_SEQUENCE=AAGGGTTAAGGGAAAACGTACC
PRIMER_LEFT_4=270,22
PRIMER_RIGHT_4=564,22
PRIMER_LEFT_4_TM=59.324
PRIMER_RIGHT_4_TM=59.665
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_00143 Ps_contig_mira-and-tgicl-ass_franssen_1635
SEQUENCE=CGGAGCTAAGAGAGTAGCTTCCAGCGCCGCCTCCGAGAGAGAGAAGAACTTTGATTCCTTTCGCTTGACATGCTTGAATCTCGGAACTAAACTTTGTGCATTCATTGGCACTTGGATTACAATGGCCAGCTAAGGTTTTAGTTGGCGGGGTTTGCCATTGCCGAAGTGGAGAGAAAGCTTATTGTTTAACAAAGTTTGGTAGTTTGTTTGTGTTGCAAGCATC
TARGET=35,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0693
PRIMER_LEFT_PENALTY=0.045851
PRIMER_RIGHT_PENALTY=0.023406
PRIMER_LEFT_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_SEQUENCE=TAAAACCTTAGCTGGCCATTGT
PRIMER_LEFT=4,22
PRIMER_RIGHT=139,22
PRIMER_LEFT_TM=59.954
PRIMER_RIGHT_TM=60.023
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.0693
PRIMER_LEFT_1_PENALTY=0.045851
PRIMER_RIGHT_1_PENALTY=0.023406
PRIMER_LEFT_1_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_1_SEQUENCE=AAAACCTTAGCTGGCCATTGTA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=138,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.1866
PRIMER_LEFT_2_PENALTY=0.045851
PRIMER_RIGHT_2_PENALTY=0.140702
PRIMER_LEFT_2_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_2_SEQUENCE=CTAAAACCTTAGCTGGCCATTG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=140,22
PRIMER_LEFT_2_TM=59.954
PRIMER_RIGHT_2_TM=60.141
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.2894
PRIMER_LEFT_3_PENALTY=0.045851
PRIMER_RIGHT_3_PENALTY=0.243575
PRIMER_LEFT_3_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_3_SEQUENCE=CATTGTAATCCAAGTGCCAATG
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=123,22
PRIMER_LEFT_3_TM=59.954
PRIMER_RIGHT_3_TM=60.244
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=0.5182
PRIMER_LEFT_4_PENALTY=0.045851
PRIMER_RIGHT_4_PENALTY=0.472356
PRIMER_LEFT_4_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_4_SEQUENCE=ACCTTAGCTGGCCATTGTAATC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=135,22
PRIMER_LEFT_4_TM=59.954
PRIMER_RIGHT_4_TM=59.528
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_00144 Ps_contig_mira-and-tgicl-ass_franssen_1637
SEQUENCE=CAATAGTCAAGCAAGGAAAGTCAAAGTACTTCTCTCTCTCGAGGCGGCGCCGGAAGGCTACTCTCTTAGCTCCGCCTGACGATGCTACACAAGTTGCAAACTATCTATGGAACAATTTCTTGGGTGGCACGTCAACTTCAAGGCCATTAGGTGACGCTGTTTTGGATGGAATTGATTTTGATATTGAAGCTGGTGGTCAACATTTTGATGAACTTGCAAGAGCACTAAATGGTTTTAGTGCACAAAGAGGGTTTACTTGTAACTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTGGGTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGACTAAACAAGTGTTCTTAGGTGTTCCTGACTAATGCTGCTGCTGCTCAGTGGTGTCTTTATTCCTTNTGGTATTTGGTTTTGTACTTTTCCTACCGTTAACGTTTCCTTCCTGTACTTAAGTTAAGT
TARGET=31,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.2960
PRIMER_LEFT_PENALTY=2.194342
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_SEQUENCE=ATCCAAAACAGCGTCACCTAAT
PRIMER_LEFT=4,22
PRIMER_RIGHT=166,22
PRIMER_LEFT_TM=57.806
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=2.4401
PRIMER_LEFT_1_PENALTY=2.338413
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=GTCAAGCAAGGAAAGTCAAAGT
PRIMER_RIGHT_1_SEQUENCE=ATCCAAAACAGCGTCACCTAAT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=166,22
PRIMER_LEFT_1_TM=57.662
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=2.6061
PRIMER_LEFT_2_PENALTY=2.194342
PRIMER_RIGHT_2_PENALTY=0.411756
PRIMER_LEFT_2_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_2_SEQUENCE=CTGTGGTGCTGCAGTTACAAGT
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=275,22
PRIMER_LEFT_2_TM=57.806
PRIMER_RIGHT_2_TM=60.412
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=2.6225
PRIMER_LEFT_3_PENALTY=2.194342
PRIMER_RIGHT_3_PENALTY=0.428205
PRIMER_LEFT_3_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_3_SEQUENCE=GTGCTCTTGCAAGTTCATCAAA
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=224,22
PRIMER_LEFT_3_TM=57.806
PRIMER_RIGHT_3_TM=60.428
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=2.6682
PRIMER_LEFT_4_PENALTY=2.194342
PRIMER_RIGHT_4_PENALTY=0.473868
PRIMER_LEFT_4_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_4_SEQUENCE=ACCTAATGGCCTTGAAGTTGAC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=57.806
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_00145 Ps_contig_mira-and-tgicl-ass_franssen_1637
SEQUENCE=CAATAGTCAAGCAAGGAAAGTCAAAGTACTTCTCTCTCTCGAGGCGGCGCCGGAAGGCTACTCTCTTAGCTCCGCCTGACGATGCTACACAAGTTGCAAACTATCTATGGAACAATTTCTTGGGTGGCACGTCAACTTCAAGGCCATTAGGTGACGCTGTTTTGGATGGAATTGATTTTGATATTGAAGCTGGTGGTCAACATTTTGATGAACTTGCAAGAGCACTAAATGGTTTTAGTGCACAAAGAGGGTTTACTTGTAACTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTGGGTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGACTAAACAAGTGTTCTTAGGTGTTCCTGACTAATGCTGCTGCTGCTCAGTGGTGTCTTTATTCCTTNTGGTATTTGGTTTTGTACTTTTCCTACCGTTAACGTTTCCTTCCTGTACTTAAGTTAAGT
TARGET=464,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4641
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.460533
PRIMER_LEFT_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_SEQUENCE=GAAGGAAACGTTAACGGTAGGA
PRIMER_LEFT=395,22
PRIMER_RIGHT=539,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.539
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.4649
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.460533
PRIMER_LEFT_1_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_1_SEQUENCE=GAAGGAAACGTTAACGGTAGGA
PRIMER_LEFT_1=399,22
PRIMER_RIGHT_1=539,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.539
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.5713
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.567736
PRIMER_LEFT_2_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_2_SEQUENCE=GGAAACGTTAACGGTAGGAAAA
PRIMER_LEFT_2=395,22
PRIMER_RIGHT_2=536,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.432
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.5721
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.567736
PRIMER_LEFT_3_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_3_SEQUENCE=GGAAACGTTAACGGTAGGAAAA
PRIMER_LEFT_3=399,22
PRIMER_RIGHT_3=536,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=59.432
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.9059
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.902354
PRIMER_LEFT_4_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_4_SEQUENCE=AGGAAACGTTAACGGTAGGAAA
PRIMER_LEFT_4=395,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.098
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_00146 Ps_contig_mira-and-tgicl-ass_franssen_1639
SEQUENCE=TTTAGTGCTCTTGCAAGTTCATCAAAATTTGTTTTGACCACCAGCYTCAATATCAAAWWCAATTCCATCCAAAACAGCGTCACCTAATGGCCTTGAAGTTGACGTGCCACCCAAGAAATTGTTCCATAGATAGTTTGCAACTTGTGTAGCATCATCAGCGGAGCTAAGAGAGTAGCTTCCGGCGCCGCCTCCGAGAGAGAGAAGAACTTTGATTTCCTTTCGCTTGACATGCTTGAATCTACGGTAAACTTAAACTTTGGGCTATTTCAGTTGGACCTAACGTTTGGGTAATTTAACAAGTTCGGGCCCTAAGGTCCGTTTAAAAGGGGTTTTTTAGGGTTTTCGGGGTTACCGGGGGGGGTTTTTTACGGACCCC
TARGET=193,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00147 Ps_contig_mira-and-tgicl-ass_franssen_1667
SEQUENCE=TTGNTACGGAGANAATCTTGACTNTTACTCTCAGAGGCCATTCGGATCTTCACTCTCGTTGTCATCAANCCTTCTCGACACCATCGCAGCAGTCCTTAGTATTTGGCTTTTTYCTTCAAAAATAAAATAAATGTGAATCACGGTTCACCTACTTCGGTTGACTCATCTATATATGAGYGTGACACATGTATAATATACTACTACTACTATCAATGGATTTCAATCCTTACTCGACTCACGCATTACTTTTCAAATCAAATAACACTGTGTCTTATTCTTTTAAAGTAAAAAAAGT
TARGET=196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00148 Ps_contig_mira-and-tgicl-ass_franssen_1671
SEQUENCE=TACTCGAATTTGGCTGCCATACAATGCAACAAAACTAAAAGAAGAGTAAAAAAGGAAGTAGTAGCTGAAATGAAAATAGTGATTTGTTGTTGTTGTTGGTCT
TARGET=84,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00149 Ps_contig_mira-and-tgicl-ass_franssen_1716
SEQUENCE=TTYTTGTATGAATAATATCAATTTATATAATAGTCAACCGAAGAAAAGATAGGAAATTTAAGAATTGTGAGACAAAAAAACAACATAACATAACATTATTAACTTCACATGTTACTACAAACAGGTGAAGGAATTAATAGAGTTACTACTCATTGTAGAAACAAACATAACATGATATCATATCATAGCATAGCAGTAACAGAAATAGTAAAAATTAAGCAGTGAGATCTTCTTCTGTGNGTCTCAATAAACAACATCAAGAGGGTAGGANANCAACANAAGTAANAAA
TARGET=82,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00150 Ps_contig_mira-and-tgicl-ass_franssen_1736
SEQUENCE=TCGGGGAAAGACACCAACACATTTCCCATCATCATCTTCTTCTTCTCCGATAGGAAACTTTCAGTTCTCCAACCCCCCCAAAAAAACTAGACCAAACCCCTTAAAACGTTCTTCGATATGACCGTCGGAGGCCAACCCTCTGGCCGTATTGTCATGGAGCTTTACGCTGATACCACTCCTCTCACCGNTGATTAACCTTCCGTGCACTCTGCCACCGGNGAAGAAAGGNGTCGCATAGGCGGGAA
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6188
PRIMER_LEFT_PENALTY=0.516652
PRIMER_RIGHT_PENALTY=0.102129
PRIMER_LEFT_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_SEQUENCE=TGAGAGGAGTGGTATCAGCGTA
PRIMER_LEFT=7,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=60.517
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.6567
PRIMER_LEFT_1_PENALTY=0.516652
PRIMER_RIGHT_1_PENALTY=0.140098
PRIMER_LEFT_1_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_1_SEQUENCE=AGAACGTTTTAAGGGGTTTGGT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=111,22
PRIMER_LEFT_1_TM=60.517
PRIMER_RIGHT_1_TM=60.140
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.6567
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=0.140098
PRIMER_LEFT_2_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_2_SEQUENCE=AACGTTTTAAGGGGTTTGGTCT
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=109,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=60.140
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.7065
PRIMER_LEFT_3_PENALTY=0.516652
PRIMER_RIGHT_3_PENALTY=0.189810
PRIMER_LEFT_3_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_3_SEQUENCE=AGTGGTATCAGCGTAAAGCTCC
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=176,22
PRIMER_LEFT_3_TM=60.517
PRIMER_RIGHT_3_TM=59.810
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.7065
PRIMER_LEFT_4_PENALTY=0.516652
PRIMER_RIGHT_4_PENALTY=0.189810
PRIMER_LEFT_4_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_4_SEQUENCE=AGGAGTGGTATCAGCGTAAAGC
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=179,22
PRIMER_LEFT_4_TM=60.517
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_00151 Ps_contig_mira-and-tgicl-ass_franssen_1741
SEQUENCE=ATCTTAGTCGATTGAATACAGGTTACACATATCTAAATATTTACATTGCAACACACAAATAATTGGCTGAGTTWRTAATAACAAAAAGGTCACAATTTCTTGTTAACTTCAAACATTGTTCCATGCCTAAATAGATGATTTCAATACCAACATTAAGTTAGCACCAAATGAAATTGAACCTAGATTTCTGAAACAAGATCTCAGATTTGACTCTCATGAATGAATAACATGTTAACTGTTTTATTCACAACTAAACTTGCAGCTATATATATTGTAGTTTTCACGAGATTCTAAATTACTAGAGACAATTATGCAGGAAATCAATGAAGGAATTTGCTTTGAGACTAGAGAAACTAGCAGAGGAGCTACTAGACTTGTTATGTGAAAATCTTGGACTAGAAAAAGGGTACCTCAAAAAGGCCTTCTATGGATCAAAAGGACCAACTTTTGGCACCAAGATAGCAAACTACCCTCCATGTCCAAAACCCGATCTAGTGAAGGGTCTCCGCGCACACACCGATGCCGGAGGAATCATCCTCCTTTTCCAAGACGACAAAGTCAGTGGCCTCCAACTTCTCAAAGATGGCATCTGGGTAGATGTTCCTCCCATGCATCATTCAATTGTCATCAACCTCGGTGACCAACTAGAGGTAATTACCAATGGTAAATACAAAAGTATAGAGCACCGTGTGATAGCACAAAGTGATGGAACAAGAATGTCCATAGCCTCATTCTACAACCCTGGTAGTGACGCTGTTATCTACCCAGCACCAACATTGATAGAAGAAAACAATGAAATTTACCCAAAATTTGTGTTTGAGGATTACATGAAACTCTATGCTGGGTTAAAGTTTCAGGCCAAAGAACCAAGATTTGAAGCATTTAAGGAATCAAATGTTGTTAATTTGGGACCAATTGCAACAGTTTAATTATGTGTTACTATAAATAAATATATTAAACATGTTTGTGTAAGGGGAAGCAAGCAAAGTAAAAAAAGGTTAAAGTGAAGTGTATTAGTTTCTCAAATTAGTATATATAGTTTTGTTTACTTATAGTGAGTGATGTGATGTTATTCCTTGTAACGTGGAGAAAATATAATAGTATGAAATGTTCTTAATAATCATAATAATAGTATTGTTTGATTGGAGTGGAGTGGAGAAATAAWTWTTATTGGTTAATGTCTATCTAGTCATTCTA
TARGET=1144,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00152 Ps_contig_mira-and-tgicl-ass_franssen_1772
SEQUENCE=TAATAAACCACTCAATAGCAATACAAACAAAATAAAAATTGAAAATCACCAAAAGTACAATATATTAATGTTACCATAAAATGCCAATGAAGATAATTACAATCAACAAAGTGRAGGAAGAGGCTTCCAAAGGCCATCCAAAGAGTGTTTCATACACACAATCAACTCAACACAAAAACTTAGAAGAAAAAAAAGGCCTAAACATTTAACAAAAGCATCTGCATTGATTCACTCATGAGCAACAACAACAACTTGTTTACCAACATTTTGACCAGTAAACAAGCCAATCAATGCAGCAGGACCATTCTCAAGACCCTCAGCTACGTCTTCCACATACGCAATCTTTCCTTCTCTTATAAGAGGCAAAACAAACTCCAAAAACTTTGTATAAAGATGATAGTACTCAAATACAGCAAAACCTTCCACTCGAACCCTCTTATATATGATATGTGCCAAATTCGTTACACCTTCGGGTTGATCAAGATTATACTGTGAAATCATTCCACACACAGGTATGCGACCGTGGACTCTCATATTCAAAAGTACAGCATCAAGTGTCTTTCCCCCAACATTCTCAAAGTAAATGTCAATGCCTTCTGGAAAGTATTTTTTCAAAACTGCATTGAGGTCTGGTTCTTCTTTGTAGTTAAAGGCTTCATCATATCCAAATTTATTCTTCAACAGATCCACTTTTTCTTTAGTTCCGGCACTTCCAACCACATAGCAACCGTGCAATTTAGCGAATTGTCCAACAAGTTGACCAACAGCACCAGATGCAGCCGAAACGAAAACATTCTCTCCTTTCTTAGGACTTCCTACTTCAAAGAAACCTGCATAAGCAGTCATTCCCGGCATACCGAGAATTCCAGTATAGTAGGAAAGAGGAACATCAGTGTGCTCAATTTTGAAAAGTATTTGAGCTGTAGGGATCAAGCTGTACTCTTCCCATTTAGTCATACCCCAAACTAAATCATCTTTCTTATAATCTGGATCTCTAGATTCTACAACTTTAGACACTCCAAAACCTGTCAATGGCGAACCAGGGGTAAGAGCACCAAGTTCTGCAGTAGTATCCTTGGTCATAAGAATCCGCAGGTAAGGGTCACAAGACAAGTAGAGATTTTTGAGAAGAACAAGATCGTTGGAACCTTCTGGAATCTTAAGAGTTATGGTACTGTCAACTATGTTCATGTCTGATTGTTTAGGAAAACCAGTAACATAGTCCGTGAGAATCACTTGCTTGTTCCTGCTCACTTGTGCCATGTTTGGAATTGTGTAACAAATGTATTCACTGTTGGTTCTGCTGTTGGTGGTGTAGTGTGGTGTGGTGTTTATGCTGTACTTTCTCGCTGTGTAGTGTATGTAAGAGTTATGTACACGGTACGCACATGTACCTCCGATAATACTCCCC
TARGET=240,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00153 Ps_contig_mira-and-tgicl-ass_franssen_1774
SEQUENCE=ACTTGATGCTGTACTTTTGAATATGTAGAGTCCATGGTCGCATACCTGTGTGTGGAATGATTTCACAGTATAATCTTGATCAACCTGAAGGTGTAACGAATTTGGCACATATCATATATAAGAGGGTTAGAGTGGAAGGTTTTGCTGTATTTGAGTACTATCATCTTTATACAAAGTTTTTGGAGTTTGTTTTGCCTCTTATAAGAGAAGGAAAGATTGCGTATGTGGAAGACGTAGCTGAGGGTCTTGAGAATGGTCCTGCTGCATTGATTGGCTTGTTTACTGGTCAAAATGTTGGTAAACAAGTTGTTGTTGTTGCTCATGAGTGAATCAATATAGATGCTTTTGTTAAATGTTTAGGCCTTTTTTTTCTTCAAGTTTTTGTGTTGAGTTGAGTGTGTATGAAACACTCTTTGGATGGCCTTTGGAAGCCTCTTCCTTCACTTTGTTGATTGTAATTATCTTCATTGGCATTTTATGGTAACATTAATATATTGTACTTTTGGTGATTTTCAAAMAAAA
TARGET=306,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00154 Ps_contig_mira-and-tgicl-ass_franssen_1882
SEQUENCE=GGGTCGAATAACAATGTTGTTGTCCTCATCTATGTTTGTATAGATGGTGCTTAAACGTAATAGCCTTGTGAACATGAAATTAAACTAAAAAGAAACAAGAAGATGCAAATATGTTTCGTAAGAAGCTTCATGACTAATTAACAAGTACTATACATAGAGAGAGAGAGAAGAGAGTTATGGAAAAGTGATGATGAAGAGAGTGATAYAAAGAGAAAGTTTTTGTTGGTTCTCCAGCTGGGGTGTAAACATCACAAATCACAAGAATATTATTCCCTCTTCTAGAAATGGGTCCTTGAAAATAGCTTGTGGATATAAAATAACTTCACTATCTTTTCCTGGTGCTTGATCTGTGCTAGACCCATCATAGTTCCACTTAGGTAAGTTTTGCAGGGTCACTCACTGGTCCAGGAAGAGT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00155 Ps_contig_mira-and-tgicl-ass_franssen_1918
SEQUENCE=TACTGGACAAGTTGCATTTGAAGTGACATAAGTAGAAACAAACAAACTACCCCAATCAATACCCTCTTAACGGCACCCAAACCTCTACTACCCATGACCAAAGCATCAAGCTTCAAATCTCCAACAGACTCACAAATWTTCTCTCTTGCATCTCCCCAATAAACCTTCGCCACCACAGTCACCTGTTTCTGATCAGCCGCAGYATCGAGTACATCCAAAACGTCAGCATCAGGCACCACCTCATACTGGTGAGTCACGTTCTTCTCACTTTTTTGTTAGTTTGATGGCAACTTTGTTTTTGTTTTTTATATTATCTTATTATTATCCCCCCTAATCCCCTNTTGTTAGATTTGAGATTA
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00156 Ps_contig_mira-and-tgicl-ass_franssen_1954
SEQUENCE=TATAATTGAATTTTACTTTCTACCAACTTTTTTAATTTCCTAAGCGGACGAATAGGGTCCTTCAGTTGCCCTGGTATCTGCTCCAAGAATGATACCATCCTGAAAACTAAACCAACAATAGTGGTTCCAGTCTTCAAATAAGAAGGGGGTTTAAGACCCTTCTTTTCAAGCATATCATTTCTTCTGCAGAGATCGAAACTGAACCCACCCTTAGGAGGAAGATCCAATGATTTAGTCATACTCTAAACTCGAATTGAACCTGAAAATAAACCGATACCAAAAAGATACGCATCGTCAAAAAATAAAATCATAATACAAAACCCTTGATTTCGGTGATTTGAAAATGAAGTGTTTGAGAGAGAGAGAGAGAGAGAGAG
TARGET=355,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00157 Ps_contig_mira-and-tgicl-ass_franssen_1964
SEQUENCE=GTCGCTCAAAAGGATATGGTTTTGTTAGGTTCGGTGATGAAAATGAAAGGACAAGGGCAATGACTGAAATGAACGGCGTTTATTGTTCTAGCAGACCTATGCGAGTCGGTGTTGCAACGCCCAAAAAAAACTTACGGCAATCCACAACAATATTCTTCACAAGTGTTGGCTGGCGGTGGTCACTCTGCTAATGGTGCTGGTCGGCCCGAAGGCCTACCCAATCTAGGGAAGGGGTATTACTAAACAACAACAACTATTATTTGTGGAGGGTCTTGTATTCTAAG
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3862
PRIMER_LEFT_PENALTY=0.110393
PRIMER_RIGHT_PENALTY=2.275811
PRIMER_LEFT_SEQUENCE=CTTACGGCAATCCACAACAATA
PRIMER_RIGHT_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT=130,22
PRIMER_RIGHT=280,22
PRIMER_LEFT_TM=59.890
PRIMER_RIGHT_TM=57.724
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=2.4299
PRIMER_LEFT_1_PENALTY=0.154110
PRIMER_RIGHT_1_PENALTY=2.275811
PRIMER_LEFT_1_SEQUENCE=GAACGGCGTTTATTGTTCTAGC
PRIMER_RIGHT_1_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_1=70,22
PRIMER_RIGHT_1=280,22
PRIMER_LEFT_1_TM=60.154
PRIMER_RIGHT_1_TM=57.724
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=2.4616
PRIMER_LEFT_2_PENALTY=0.185822
PRIMER_RIGHT_2_PENALTY=2.275811
PRIMER_LEFT_2_SEQUENCE=GATGAAAATGAAAGGACAAGGG
PRIMER_RIGHT_2_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=59.814
PRIMER_RIGHT_2_TM=57.724
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=2.5520
PRIMER_LEFT_3_PENALTY=0.276218
PRIMER_RIGHT_3_PENALTY=2.275811
PRIMER_LEFT_3_SEQUENCE=AAAACTTACGGCAATCCACAAC
PRIMER_RIGHT_3_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_3=126,22
PRIMER_RIGHT_3=280,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=57.724
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=2.6361
PRIMER_LEFT_4_PENALTY=0.360320
PRIMER_RIGHT_4_PENALTY=2.275811
PRIMER_LEFT_4_SEQUENCE=AAGGGCAATGACTGAAATGAAC
PRIMER_RIGHT_4_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=280,22
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=57.724
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_00158 Ps_contig_mira-and-tgicl-ass_franssen_1978
SEQUENCE=TATGGTAGAAAGCCTAGATTTCTTTGAGGCTATTAAATCAGGAAGATTATGAGATAAGCTGATTCAACRATAATTCCAAAAGTGTTAATGGTGATGAAGAAGAAGAGCAGCTTCTCTTTTCACAAATGCATAGTAAATCCAAAGCATTGCACTGAAAAGTGCAACAACATAAGGAAGTGACTGAAATCCTTCAGCAGATTTTTTCTTGAAGATTATGTAAAAAGTTGGCACAGGTGAAAGAAACACTGCAAAGGAAATGATGTTGCCTAGAAGGCCAAAGACAAAAGCCAAAGTTTCACGATGCATGGCCWATTTCTTGAATCTAAAGTAAAAACTGTTAGAAACTTTGGAAATTAATTTAAGAATAAGTGTCACTCTTCTTTCTTTCTACAAAGTGACTACCTAAAACCCTTTAGKTAGAAGGTATCTACTTTTNTTTCTCTCTACTAAACTTCCCNCCCCG
TARGET=378,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00159 Ps_contig_mira-and-tgicl-ass_franssen_2017
SEQUENCE=CAGGGGAAGACCGTTGGAGCTGRTGTTATTGGAGCTATCATTGAGTGAATGAAGATTTCTATCCAACGATGCAGCACCACTCTTTAAACGGGGTTCTTTATTTATTTATTTTTAATTGCAATCCATTAATTGACTTTTGTCCCTTTTTATACARCTTGTCTGTGGTAATTATAGGTTAATTTAACTTCGGGTATGATTCTTTAAGTCAAATTAGGGTTACGTTATTTTTCCTATCAGAATGTTTTGTTTGTTGTTACTTAG
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00160 Ps_contig_mira-and-tgicl-ass_franssen_2021
SEQUENCE=GTCGGGATCATCAAACATAATACCTCAAATTCTAACAACCTTACCTTAAACCTTCTCAACCCAACAACAACAACAACAACATGGGCAGTGTTGAAATTCCGACAAAGGTTCTTACCAACACATCTGCTCAAATTAAGATGCCTGTGGTTGGAATGGGATCAGCACCTGACTTCACATGCAAGAAAGACACCAAAGAAGCAATCATTGAAGCCATCAAACAAGGTTACAGACACTTTGATACTGCTGCTGCTTATGGCTCTGAACAAGCTCTTGGTGAAGCTTTGAAYGAGGCTATTCAACTTGGTCTTGTCACTAGAGAACAGCTTTTTGTTACTTCCAAACTTTGGGTTACTGAAAATCAAATACATTGCAGTCGGGAATGAGATTCACGCAGATTCGCCCGAAGCAAGTTCGGTTCTTCCCGCATTGCAAAACATTCAAAACGCGATATCT
TARGET=62,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00161 Ps_contig_mira-and-tgicl-ass_franssen_2028
SEQUENCE=CCGGTTGCAGTTCAGCCGANCCCCTTTGGTCGCGGTGGTGGCGGCGCTGGTGCTCCTGGAAGGAGGTTTAAGTTTAAGTCTTTTTCTTAATTTTGTTATGTCAAGACTATTAGATTTGTTTTACTTTTGAAAAATGTTTTGTTATGGTTGAACTGGTTTAAGTTATTGTAATGGAATTTAATATATATATTTTTCATTCTGAATTGTATGGAATTGTATGAATGATGCTAGTTTTATGACTTTGAACT
TARGET=181,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00162 Ps_contig_mira-and-tgicl-ass_franssen_2050
SEQUENCE=TCGTTGGCCTTCGTCCCGTATCCCCCTCCGTTTCGTCCGGTGGTCGTTCCTAGCCTACGCACCGAACCTACCAACACCCAAACTAGGTCAGGTCGATGAACAAGGTTGAAAGACGCTTGTCGGTGGAACTGAAGTAGAAGACATGACTTTCCAACTCCAGTGTCTCCGATGATGATATACTTGAAGAGATATGCGTAAGACATTGTTGTGTGCGAGAGAGAACGAGAAGGAAGAGGAAAGAAAGAAAAGCGAAGGAGGGAGAAGGAGAAGGAAAAGAGAGAAGAANAAAATTAGAAATCGATTATCGAGATACAAATCGTAATTATACGTCCGTTCCAGGCGAGGACTAAATTAA
TARGET=236,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2605
PRIMER_LEFT_PENALTY=0.171757
PRIMER_RIGHT_PENALTY=0.088755
PRIMER_LEFT_SEQUENCE=ATGAACAAGGTTGAAAGACGCT
PRIMER_RIGHT_SEQUENCE=TTAATTTAGTCCTCGCCTGGAA
PRIMER_LEFT=95,22
PRIMER_RIGHT=354,22
PRIMER_LEFT_TM=60.172
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.3148
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.088755
PRIMER_LEFT_1_SEQUENCE=TGTCGGTGGAACTGAAGTAGAA
PRIMER_RIGHT_1_SEQUENCE=TTAATTTAGTCCTCGCCTGGAA
PRIMER_LEFT_1=117,22
PRIMER_RIGHT_1=354,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.3148
PRIMER_LEFT_2_PENALTY=0.225996
PRIMER_RIGHT_2_PENALTY=0.088755
PRIMER_LEFT_2_SEQUENCE=TTGTCGGTGGAACTGAAGTAGA
PRIMER_RIGHT_2_SEQUENCE=TTAATTTAGTCCTCGCCTGGAA
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=354,22
PRIMER_LEFT_2_TM=59.774
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.3389
PRIMER_LEFT_3_PENALTY=0.171757
PRIMER_RIGHT_3_PENALTY=0.167146
PRIMER_LEFT_3_SEQUENCE=ATGAACAAGGTTGAAAGACGCT
PRIMER_RIGHT_3_SEQUENCE=CTTTTCCTTCTCCTTCTCCCTC
PRIMER_LEFT_3=95,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=60.172
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.3389
PRIMER_LEFT_4_PENALTY=0.171757
PRIMER_RIGHT_4_PENALTY=0.167146
PRIMER_LEFT_4_SEQUENCE=ATGAACAAGGTTGAAAGACGCT
PRIMER_RIGHT_4_SEQUENCE=CTCTTTTCCTTCTCCTTCTCCC
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=60.172
PRIMER_RIGHT_4_TM=59.833
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_00163 Ps_contig_mira-and-tgicl-ass_franssen_2053
SEQUENCE=CAAGGCAGCATGCAAATAGCAAATAATNACGATTATTGCTGATTGGTAACAAAGTAGTGANCTTGCTCACANACGGGCTGTATGCACAGAAGAAGGCGAACAGTTTGCAAAGGAGCATGGGTTGATCTTCATGGAGGTCCTTCAGCAAAAACTGCTCAGAATGTTGAAGAGGCCTTCATAAAAACAGCTGGAACCATATACAAAAAGATTCAGGATGGAGTTTTTGATGTATCAAATGAGTCTTATGGAATAAAAGTAGGATATGGTGGAATTCCTGGACCATCGTGGGGGTAGGGATGGCCCTTCTGCTGCTGGTGGAAGCTGCTGCAGTTGAAGTGAAACATATCGCAACCTTGTTTGTTACGAACGTTGTTTCTTTATGTATTCTTCTTCTTCGTTTTGGGTTGGATTATTAGTGGGTAAAGTTATACCTAACG
TARGET=385,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0902
PRIMER_LEFT_PENALTY=0.033905
PRIMER_RIGHT_PENALTY=0.056343
PRIMER_LEFT_SEQUENCE=TAGGATATGGTGGAATTCCTGG
PRIMER_RIGHT_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT=256,22
PRIMER_RIGHT=419,22
PRIMER_LEFT_TM=60.034
PRIMER_RIGHT_TM=59.944
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.2497
PRIMER_LEFT_1_PENALTY=0.193337
PRIMER_RIGHT_1_PENALTY=0.056343
PRIMER_LEFT_1_SEQUENCE=CTTCAGCAAAAACTGCTCAGAA
PRIMER_RIGHT_1_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT_1=139,22
PRIMER_RIGHT_1=419,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=59.944
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.4941
PRIMER_LEFT_2_PENALTY=0.437791
PRIMER_RIGHT_2_PENALTY=0.056343
PRIMER_LEFT_2_SEQUENCE=CAGCAAAAACTGCTCAGAATGT
PRIMER_RIGHT_2_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=419,22
PRIMER_LEFT_2_TM=59.562
PRIMER_RIGHT_2_TM=59.944
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.5536
PRIMER_LEFT_3_PENALTY=0.033905
PRIMER_RIGHT_3_PENALTY=0.519744
PRIMER_LEFT_3_SEQUENCE=TAGGATATGGTGGAATTCCTGG
PRIMER_RIGHT_3_SEQUENCE=ACCCACTAATAATCCAACCCAA
PRIMER_LEFT_3=256,22
PRIMER_RIGHT_3=420,22
PRIMER_LEFT_3_TM=60.034
PRIMER_RIGHT_3_TM=59.480
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.6110
PRIMER_LEFT_4_PENALTY=0.554687
PRIMER_RIGHT_4_PENALTY=0.056343
PRIMER_LEFT_4_SEQUENCE=TCAGCAAAAACTGCTCAGAATG
PRIMER_RIGHT_4_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT_4=141,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=60.555
PRIMER_RIGHT_4_TM=59.944
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_00164 Ps_contig_mira-and-tgicl-ass_franssen_2054
SEQUENCE=AGTCATCCTACGCACCGAAACCTACCAACACCCAACTAGGTCAGGTCATGAACAGGTTGAAAGCGCTTGTTTCGGTTGAACTGAAGTAGAAGACATGACTTTCCAACTCCAGTGTCTCCGATGATGATATACTTGAAGAGATATGCGTAAGACATTTGTTGTGNGCGAGAGAGAACGAGAAGGAAGAGGAAAGAAAGAAAAGCGAAGGAAGGGGAAAAGGAGAAAGGAAAAGAGAGAAGAAG
TARGET=189,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0622
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.049761
PRIMER_LEFT_SEQUENCE=GTCAGGTCATGAACAGGTTGAA
PRIMER_RIGHT_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT=39,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.050
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=0.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.2609
PRIMER_LEFT_1_PENALTY=0.211105
PRIMER_RIGHT_1_PENALTY=0.049761
PRIMER_LEFT_1_SEQUENCE=CCTACCAACACCCAACTAGGTC
PRIMER_RIGHT_1_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.789
PRIMER_RIGHT_1_TM=60.050
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.2653
PRIMER_LEFT_2_PENALTY=0.215550
PRIMER_RIGHT_2_PENALTY=0.049761
PRIMER_LEFT_2_SEQUENCE=ACACCCAACTAGGTCAGGTCAT
PRIMER_RIGHT_2_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=59.784
PRIMER_RIGHT_2_TM=60.050
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.4046
PRIMER_LEFT_3_PENALTY=0.354881
PRIMER_RIGHT_3_PENALTY=0.049761
PRIMER_LEFT_3_SEQUENCE=ACTTTCCAACTCCAGTGTCTCC
PRIMER_RIGHT_3_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_3=97,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=59.645
PRIMER_RIGHT_3_TM=60.050
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.5324
PRIMER_LEFT_4_PENALTY=0.482662
PRIMER_RIGHT_4_PENALTY=0.049761
PRIMER_LEFT_4_SEQUENCE=TCCAGTGTCTCCGATGATGATA
PRIMER_RIGHT_4_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=60.483
PRIMER_RIGHT_4_TM=60.050
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00165 Ps_contig_mira-and-tgicl-ass_franssen_2122
SEQUENCE=GTGGGGGGATTCATTTTTCTCTCTCTCTCAAAAGCTTTTCACTTGAATTGAGTTGATGATGGCTTCCGAATCTGAAGTTTCTGAACTTTCTTATATCTTCGAAAGAATGATCAGAAACAGAGACATGTCTCTGTTGTTGCCCTTCATCCAAGGTCTTTCCGAATCTTCAATTCAAAGACACAGGGACAACCCAGTTCAAGAATCTTCGAGCAACGAAGAAGATTCCAACCGTCAAAGAATCATCTTGGTGAACCCTTCTACACAGCGCATGATCGTGATCGAAGGAGTTTCAAGTCTGGAAACTCTGTTTCAAGGAAACTTAGAAAAGTAACTAGAAAGAAAGGTCAGCCACCAGCTTCAA
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00166 Ps_contig_mira-and-tgicl-ass_franssen_2127
SEQUENCE=ATGAGGAAGGCCTAGGTCAAAGTACTTGATTGTCCAACTCCAAGAAAGGAAAAATGAGCTTATCCTTGATTGACTTCCAGATAACTCGGGTCATTTCATCTCCGTCCATCTCAACGATGGGACTGGCGACTTTGATTTTCNGGAATGCCATGGTGGTGATCGGAAGAAGAGAAGAGGATGAAGATGCGGGARGAGAGTGKTAGGTKARGAGAAAGTAAGTAGGACGGACGGACGGACCGGTACCCGGTACCGTAGTTACCTCGGTCGGTTACGGTTACGTAACCCTACCGTTCGG
TARGET=222,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00167 Ps_contig_mira-and-tgicl-ass_franssen_2135
SEQUENCE=TYWCTTGGCGATCCGGAACCTAGTTGTAGCAGCTTGTGTCTGCAATTGAGAGCTCAAGTCCTCTATTTTTGTCTCCCCAAATATAACGTAGGTCTCAGAGTTTGGACTCTTGAAGACATCAGGTTTTGAGATGAAAAACATTATATTTTTTGTTCTCTTGATTGTTACCCTGCTAACACCATCAATTGGTTTTAGTCCCAGCTTCAACATTGCTTTGCGACTCTTCTTCTCACTTCTRCTCTGWCKTTGATACCTWCWGCTWCCACCTTGAGCGGCGTCATCTTCCTCGTCATCGGATTCGTCTTTCTCGTCGTCGTCGTCATCCTCGACAATAGGAGCGTTCGTTTTTCTTCCGTGGGGAANATGTCGTCCTTTCGTTGGGTAGGGGTTTTCGTTTTACCAGTCCGGACGGGAGGGGTAGTTTGGTTCGGGACGGGTCCGGGTTTCCGGTCGGGTCGGTT
TARGET=308,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00168 Ps_contig_mira-and-tgicl-ass_franssen_2156
SEQUENCE=CTTTGCCACTGAGGTGAGGTTTACAGAAGGCATTGAACCAGAAGCTGGTTCGGTACTGTGGAAGTAGAAGAAAAGTTAGAAGAAGAGAAGAGTGTGAGTTTTCAGGAGAGAGAGAGAGAG
TARGET=106,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00169 Ps_contig_mira-and-tgicl-ass_franssen_2161
SEQUENCE=GTTCGGGGTTGGCGCGTGCGTTTTGTTGKATATAAAAAGACAATGATGTATCAGACGAARTGGAGGGATTTTCTTCGAAATCTAATTCTTCTTCTTCTTCTAGTTCTAGATTCCGACTCGCCGTGTTGACTCGGTCCGGGTCGGGGTTTTACTACGACTCATTTCTGAATCTTGATTTTCAATTCTCACGCGGATCTAACTCCTCCACAACCGAGGAAAACAAAGGACTTATCACAATGAAGGCATTGATTCTGGTTGGAGGGTTTGGAACTAGGTTGAGGCCATTGACACTCAGTGTCCCTAAGCCTCTTGTTGATTTTGAACTAACAAACTCATGATTTTGCATCA
TARGET=86,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00170 Ps_contig_mira-and-tgicl-ass_franssen_2237
SEQUENCE=GTAACATGAAAATTGAAATGATGCAAATAAGCATAACACAAATAAGAAACAATATACAAACTTAGTAGTGGAGGGGTAATTTAAAAGAATTWCATCTAAATCATACTCAACCGAGAAAWTTACAAAAACTCCCCTCGTDGAAATCCACGCCTTCAATTTGCTCAATTTCATCCTCCCTCATAAACTCTCTCAACCTCTCCTTAAAAATCCCAACTGCTCCTCCAAAAAGCTCTTCTGAGAATCAAGTTCAACAACATTCCACCTCAACCGACTACAACTACCTACCTTACCCGTACTACTACTACTTCTTCTCCTCCTCCTCCACCACCTACCCTACCCGTAACCTACGTACTTCCGTCCTACCTAACTACCTACCTAACCCGTAACCTTACCTTT
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00171 Ps_contig_mira-and-tgicl-ass_franssen_2237
SEQUENCE=GTAACATGAAAATTGAAATGATGCAAATAAGCATAACACAAATAAGAAACAATATACAAACTTAGTAGTGGAGGGGTAATTTAAAAGAATTWCATCTAAATCATACTCAACCGAGAAAWTTACAAAAACTCCCCTCGTDGAAATCCACGCCTTCAATTTGCTCAATTTCATCCTCCCTCATAAACTCTCTCAACCTCTCCTTAAAAATCCCAACTGCTCCTCCAAAAAGCTCTTCTGAGAATCAAGTTCAACAACATTCCACCTCAACCGACTACAACTACCTACCTTACCCGTACTACTACTACTTCTTCTCCTCCTCCTCCACCACCTACCCTACCCGTAACCTACGTACTTCCGTCCTACCTAACTACCTACCTAACCCGTAACCTTACCTTT
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00172 Ps_contig_mira-and-tgicl-ass_franssen_2262
SEQUENCE=CCGTTTTAGAACAARACAAAACATTAGCCAAGAGCAGCAAAATATTTGGTAGCAAGACATTTTATTCATAAAATAGACAATCATCTACATAAGATGATCAAATCATCAGCATAGCATCCTACATAAATGTTATCATCATCCAATTTTCTACTCGTCATCATCCTCCTCCTCACTTTCTTCCTCATCATCATTCTCATCATGCACCTCAGATTCTGATTTCTCGGATTCTTCTTCTTCTTCAACAGCAGCTGGACCTTCAGCTTGTTTCTTGTTATAGGACTTCATGCTCTTCGCATATTCAGCCTTCCTTTTCTCTGCCTTAGCAGCATATGGTGCTTTCTCAGCTTCAGTCATTGACTTCCTATTTTCGACCCCGCAGCTTTGCCCACTGCAGACACAGCTTTGTTGTCTAGGGTTTTCCTTTTGACTGCTTCCTAAAGTCCTCCATAGAAAACGAAGAAAGCACTTGGAGGTCTCTTCGGCTTGGTTACGGGTTCCCTTTTTCGGGTTTTCCCCTTTTTCCCCCGTTTGGGGTCCTTTCGGGGTTTTTCCCTTTTCCCCGGTCCCCGTTTTTGGGTCGGGGCCGGGGGGTAACCGTCCCCTTAACCCCCCTTTTTTCCGTTTTAAACCTTTTCCCCGAAACCCCCGGGTACCTTAAAACGGGTTTCCCCGTTTTTTTCCCGGGGTTTTTTTACCCTAAACGGGTTTTCCCCTTTTTTTTT
TARGET=226,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00173 Ps_contig_mira-and-tgicl-ass_franssen_2264
SEQUENCE=GAAAAGGAAGGCTGAATATGAGAAGAGCATGAAGTCCTATAACAAGAAACAAGCTGAAGGTCCAGCTGCTGTTGAAGAAGAAGAAGAATCCGAGAAATCAGAATCTGAGGTGCATGATGAGAATGATGATGAGGAAGAAAGTGAGGAGGATGATGACGAGTAGAAAATTGGATGATGATAACATTTATGTAGGATGCTATGCTGATGATTTGATCATCTTATGTAGATGATTGTCATTTTATGATAAAAATGTCTTGCTACCAATATTTGCTGCTCTTGGCTTAATTGTTTTGTCTGTTACAAAACGTGAAATAAGTTAAAATGGAGGGAGTACTTAAACTTTTTATTTGTACTTTAACGGCCTAACTTCTTTAAGTTAAGAGTATTTGTTTTATTCGTTTTAGGTAGTAGTTCTTTTAGTATTTTCA
TARGET=74,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2721
PRIMER_LEFT_PENALTY=0.205226
PRIMER_RIGHT_PENALTY=0.066849
PRIMER_LEFT_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_SEQUENCE=TCAAATCATCAGCATAGCATCC
PRIMER_LEFT=41,22
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=60.067
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.2807
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.075444
PRIMER_LEFT_1_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_1_SEQUENCE=AAACAATTAAGCCAAGAGCAGC
PRIMER_LEFT_1=41,22
PRIMER_RIGHT_1=290,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.925
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.3460
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.140792
PRIMER_LEFT_2_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_2_SEQUENCE=ACTCGTCATCATCCTCCTCACT
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=60.141
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.8543
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.649076
PRIMER_LEFT_3_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_3_SEQUENCE=CTCGTCATCATCCTCCTCACTT
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=159,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=60.649
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.8825
PRIMER_LEFT_4_PENALTY=0.205226
PRIMER_RIGHT_4_PENALTY=0.677241
PRIMER_LEFT_4_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_4_SEQUENCE=CCTCCTCACTTTCTTCCTCATC
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=148,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=59.323
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_00174 Ps_contig_mira-and-tgicl-ass_franssen_2265
SEQUENCE=CTCTTCACTCGGTTCATCATGAAGGAGGAAAGTCCAAGGTGAATCGAGAAAGCTGAACGAAGCTTGCGGTGAATAAGAAGGGTGCTCCCGCCACAAAGGGCGGAAAGAAACCAGCCAAGGGAAAGGAACCAAAGGACCCTAACAAGCCGAAGAGACCTCCAAGTGCTTTCTTCGTTTTCATTGGTAGGTACTTTTAGGTAAGCAGTTCAAAAAGGAAAACCCTGACAACAAAGCTGTGTCTGCAGTGGGCAAAGCTGCTGGGGCAAAATGGAGGTCAATGACTGAAGCTGAGAAAGCACCATATGCTGCTAAGGCAGAGAAAGGAGCTGAATATGAGAGAGCATGAAGTCCTATAACAAGAAACAAGCTGGAACGGTCCAGTCGTGGTCGTTGTTAGGAAAGAAAGAAA
TARGET=398,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_00175 Ps_contig_mira-and-tgicl-ass_franssen_2267
SEQUENCE=TCGGGGGTAAACTASACACAAACAAACAATCACATAACTAGAAAATGGAGAGCACACAGAGAAGTTTAAGCTTGTTGGTACTTTGTTTCCTGCTTATGGGAACAACAATGTTAGTGAGTGGTCAGAGTGCAAACAACGTAAGAGCAACATACAATAACTACAACCCTCAGAACATAATGGATTACAACAGNGCCNGTGTGTACTGTGCCTACCGTGGGATGACCTAAACCGACGTTCC
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00176 Ps_contig_mira-and-tgicl-ass_franssen_2302
SEQUENCE=AAGACAGTGGGTATCAACGCAGAGTCAAAAAATTTATGAGTTGAGGGTGCTTTTTCACTTCTGACCTCCCTCCCTCCAAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAAGTATTTTGAAAGGAAAGGAAAGATAAACCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCAGAGAGAGTCACGAGAAGTTTAGATGAGAGCTTGGAAAGGTTGCAG
TARGET=65,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2656
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=GTTGAGGGTGCTTTTTCACTTC
PRIMER_RIGHT_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT=39,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.3197
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=AGGGTGCTTTTTCACTTCTGAC
PRIMER_RIGHT_1_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.3197
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=GAGTTGAGGGTGCTTTTTCACT
PRIMER_RIGHT_2_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3892
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=TTGAGGGTGCTTTTTCACTTCT
PRIMER_RIGHT_3_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.6076
PRIMER_LEFT_4_PENALTY=0.151106
PRIMER_RIGHT_4_PENALTY=0.456523
PRIMER_LEFT_4_SEQUENCE=GTTGAGGGTGCTTTTTCACTTC
PRIMER_RIGHT_4_SEQUENCE=CGTGACTCTCTCTGCACAAAAT
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.151
PRIMER_RIGHT_4_TM=59.543
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_00177 Ps_contig_mira-and-tgicl-ass_franssen_2302
SEQUENCE=AAGACAGTGGGTATCAACGCAGAGTCAAAAAATTTATGAGTTGAGGGTGCTTTTTCACTTCTGACCTCCCTCCCTCCAAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAAGTATTTTGAAAGGAAAGGAAAGATAAACCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCAGAGAGAGTCACGAGAAGTTTAGATGAGAGCTTGGAAAGGTTGCAG
TARGET=166,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2656
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=GTTGAGGGTGCTTTTTCACTTC
PRIMER_RIGHT_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT=39,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.3197
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=AGGGTGCTTTTTCACTTCTGAC
PRIMER_RIGHT_1_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.3197
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=GAGTTGAGGGTGCTTTTTCACT
PRIMER_RIGHT_2_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3197
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=GGTGCTTTTTCACTTCTGACCT
PRIMER_RIGHT_3_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.3852
PRIMER_LEFT_4_PENALTY=0.270774
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=TTTTTCACTTCTGACCTCCCTC
PRIMER_RIGHT_4_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=59.729
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_00178 Ps_contig_mira-and-tgicl-ass_franssen_2305
SEQUENCE=GGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATACTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCYTMCCCAAGCAAACGCCGAGACATGGATCTGATAGAAGCTGATGATGGCTAGAATTTAACGAAAAGGTGGGTAAGTAATTTAATACGAAATTGGAACTTAACCCAAGTTCGGAACCGAAAAGGTAACCGGTACCTTTTTCCCGGTTTTTTTTACGGGGTTAAACCCGGTTTAAAAACGTTTTTTTAACCCCCGGAAAACCCCGGGGGGTTTAAAAAACCCCCTTAAAAAAAACGGGTTAAACCCCCGG
TARGET=59,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00179 Ps_contig_mira-and-tgicl-ass_franssen_2305
SEQUENCE=GGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATACTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCYTMCCCAAGCAAACGCCGAGACATGGATCTGATAGAAGCTGATGATGGCTAGAATTTAACGAAAAGGTGGGTAAGTAATTTAATACGAAATTGGAACTTAACCCAAGTTCGGAACCGAAAAGGTAACCGGTACCTTTTTCCCGGTTTTTTTTACGGGGTTAAACCCGGTTTAAAAACGTTTTTTTAACCCCCGGAAAACCCCGGGGGGTTTAAAAAACCCCCTTAAAAAAAACGGGTTAAACCCCCGG
TARGET=69,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00180 Ps_contig_mira-and-tgicl-ass_franssen_2324
SEQUENCE=GATTCTTATTTTTTCTATCACCGCTTCAAAAATCAGKAGCATTGCAATCATCATCATCATCCATGGCTCCCAAAAGGGTTCTTCTCCTTTGCGGTGACTTCATGGAAGACTATGAAGCTATGGTTCCTTTTCNGGCATTGCAAGCATTCGGTATCACCGTCGATGCTGTTTCGTACCCGGTAAAAATCCGGTGAAGTCTGCCGCCACCCGCCGTCCATATGTTCCG
TARGET=47,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00181 Ps_contig_mira-and-tgicl-ass_franssen_2341
SEQUENCE=CAAAACCTCAAGACTGATTGGAAGAAGGTGGTTTCCGTTAGTTCAACATCATATTCCTGATTTTTCTGGTCATTGTATACTCCATCGGCTGCTGCGCATTCAGGAATAACAGGCAGGACAATTGGAAGAGATATTAGGCTCTTGAGCATTATTAGCATGTAGATGTATTTAGGCTATATTGGTTTATATGCTGTATTTGTGTGTGTGTATTCTATTACTTTGTTTTACAAGTTACTAGTTTGTACTTTGTTTAGTTAGTTAAATTGTACTTAATTATAGTATANAGTNTGTGTATTAGAATTAACGTAAAACTTAAAAAAACT
TARGET=198,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00182 Ps_contig_mira-and-tgicl-ass_franssen_2342
SEQUENCE=GGCTGCTGCGCATTCAGGAATAACAGGCAGGACAATTGGAAGAGATATTAGGCTCTTGAGCATTATTAGCATGTAGATGATTTAGGCWATATGGTTTATATGCTGTATTTTGTGTGTGTGGATTTCATTACTTGTTTTTCAGTACTAGTTTGACTTGTTTGTAGTAAATTGACTTAAACTTTATAGTATTACAGTATGTGGTATTAGWAATTGAWACTTACWAAAYKTAACCGTTTGGTGACTAACTTACCTTAAGTTAGTTTAAACTAAAACCAAAAAAAAAAC
TARGET=111,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00183 Ps_contig_mira-and-tgicl-ass_franssen_2343
SEQUENCE=GATCTTTCTCAACAATCAAAACAACCATTTCAATTTTTCCATTACTATAAAACAACATTTTCTCTTTCACAAAAAAAAAACCCTAAAACCCATTTCATCTTCATCTTCATCTTCATCTATCAATTCAATTCAATTCAACCATGTCTCGATTCAGTAACAACCTAATCGGAATCCTCAATTTCCTAACCCTACTCCTCTCAATCCCAATTCTAGTCATGGGAGTATGGCTCCACAAACAGTCCACCACGGAATGCGAACGCTTCTTAGACGAAACCCATTATTCGCCCTTTGGKCGTTTTCCTCTTGGTTGTTTCT
TARGET=120,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00184 Ps_contig_mira-and-tgicl-ass_franssen_2346
SEQUENCE=TAAGTGGGTGGTGGTTGTTGATGTTGTTGTAGACAAAGGAATGAGCATCATGTTTTTTCTATATGTTTGACCCAACATGTGAGAATGCTAAAAATTTGCTTAATCAAGTTGTTTCCTTCATCAAAAGAACTTGAAAATATGAGGGTGGGAGTGATGCTTCCTTTATTTTGTTTTCTATGTTCTATTATCATTTTAAATATTTTTATGTACTCTTTCTTGTGTGTTAAAGAAAAAAATCCAAAAAGTTAATAAAATAAAATAAATTTTTAACATAAGATTTATGRTT
TARGET=248,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00185 Ps_contig_mira-and-tgicl-ass_franssen_2349
SEQUENCE=CAACGCAGAGTACGCGGGATACCAACACAAACACAAACCAATTCCTACTGTATCTCAATACAAAAAATGGCCACACTATTTTCATCTTCATCTCTTCATTCAATCCGTCTTTCTTCTTCTACATTCCCCGTTTCTTCCTCTTCTTCTCAATCCCTTCAATTTTGCNCTTTTCCATGCCACCTCGTTGAGCNCTGTCAATAAAAAAGTAACCGTTGTTTTACTTGTTGGTTAAGGCAGTGGAAGATAGAAACCCAACCAAACAAACAAAGAAACG
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00186 Ps_contig_mira-and-tgicl-ass_franssen_2384
SEQUENCE=GGGTATTGATTCAAGATTAGATGAAAGTTTACATGATGTTTTAGTAATGAATTGTATCAATTCATTTCAATTTGACAAGTCAAGCTTATTTGTATCAAGTATTAAATTGCACATGGTATTTGGACAAAGTGAGTCTTATTTGTATAATGTAATGTAATTAGTTAAATTTGGTTTTATTTCTTATTTTTGAAGACAAGTAACTTCACAATAACTAACTAAAACTATTTATTTATTACTATTAACTAACCAAAAC
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00187 Ps_contig_mira-and-tgicl-ass_franssen_2396
SEQUENCE=AACGTTTWCGGGGATAACAACTTAACTAACCATTGACACTTAGAGCATCCATCCATCATGTCGCTCGCTTCCATTTCTTCGGCATTCTCCTCATTCTCAATTTCATTACCCTCCTCCTCCCGTTCTACTTCACTTTCATTTCTAACTTACTAATTTCCTAAGAAATACCCTCCTTCTCATCCTCAATTAGCAGCAACATGATAGACCAAAAAGACGAGTGGTTGT
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00188 Ps_contig_mira-and-tgicl-ass_franssen_2399
SEQUENCE=GGAAATCCAAAATCCCCAAACCACACCCAACCATTCTTCCATTGGTCTTCACGTCCATTCATTTCCACCTCACATCACTACAATAACCTCAAAAAACTTCCTTCATCCACTTCACTCTCTCACTCTCCCTCTCTCTCTCTCTACAATGGCTTCAGCTACAGCACCCACAACTCTCTCCCTCCTCCCCACCGCAAAATCCTCCACCTCATCAGCAACCCCATCTTCCTCCCGTGTCCACCTCTCCTCCTCATTCCTTGGCGCCCGTCCGCTCCGCCGACTAGGTTTCTCGGCTGCCGATCCCTTGCTGGCATTGCGAGGTGGCGTCCAAGGTGAGGACAGTGAGGG
TARGET=129,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2053
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.984274
PRIMER_LEFT_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_SEQUENCE=GAGAGAGTTGTGGGTGCTGTAG
PRIMER_LEFT=27,22
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=59.016
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=1.2866
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=1.065587
PRIMER_LEFT_1_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_1_SEQUENCE=GAATGAGGAGGAGAGGTGGAC
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=252,21
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.066
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=1.5359
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=1.314939
PRIMER_LEFT_2_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_2_SEQUENCE=AAGGAATGAGGAGGAGAGGTG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=255,21
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=1.5359
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=1.314939
PRIMER_LEFT_3_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_3_SEQUENCE=CAAGGAATGAGGAGGAGAGGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=256,21
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.685
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=1.6033
PRIMER_LEFT_4_PENALTY=0.619003
PRIMER_RIGHT_4_PENALTY=0.984274
PRIMER_LEFT_4_SEQUENCE=ATTGGTCTTCACGTCCATTCAT
PRIMER_RIGHT_4_SEQUENCE=GAGAGAGTTGTGGGTGCTGTAG
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=60.619
PRIMER_RIGHT_4_TM=59.016
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_00189 Ps_contig_mira-and-tgicl-ass_franssen_2405
SEQUENCE=CCCGTTTGTCGTGTCTCAGCATCAATACTGAAGAAAAATAAGACAGAATTTTTAGTTTAACCTTACCCAACTTAGATAACCGAACCGGAAAACCTAGCTACGAAAACCGAACGCTCCTCCCAATAACACCCCTCCCAACCAACCAAAACCTACCTACTCCGTCAACAACACCATCAACAACACCATCAACCACTCCATCAACCCCATCTTCTGAGACAAATACAACTTCTCCACCACCACCAAAGGATAGTGGAGCTGTCGGTGTTTCTAATGGTGTTGGTATTTTGATGGGATCTTTGTTTTTGGCTTTGGGTTTCATGGGCTAGGGAAGAGACAGTGCAATGATTATTATTGATCATTTTAATTTTATGTGTTATTGGTTTTTGTTGAATTTGGTTTAATTTTATTTAATGATTTGAGATGTACATCTTATCTTCATCTTGATATGTATCTATTTTAATGAAAAAAACTTCATTTWAAAATTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00190 Ps_contig_mira-and-tgicl-ass_franssen_2405
SEQUENCE=CCCGTTTGTCGTGTCTCAGCATCAATACTGAAGAAAAATAAGACAGAATTTTTAGTTTAACCTTACCCAACTTAGATAACCGAACCGGAAAACCTAGCTACGAAAACCGAACGCTCCTCCCAATAACACCCCTCCCAACCAACCAAAACCTACCTACTCCGTCAACAACACCATCAACAACACCATCAACCACTCCATCAACCCCATCTTCTGAGACAAATACAACTTCTCCACCACCACCAAAGGATAGTGGAGCTGTCGGTGTTTCTAATGGTGTTGGTATTTTGATGGGATCTTTGTTTTTGGCTTTGGGTTTCATGGGCTAGGGAAGAGACAGTGCAATGATTATTATTGATCATTTTAATTTTATGTGTTATTGGTTTTTGTTGAATTTGGTTTAATTTTATTTAATGATTTGAGATGTACATCTTATCTTCATCTTGATATGTATCTATTTTAATGAAAAAAACTTCATTTWAAAATTT
TARGET=231,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00191 Ps_contig_mira-and-tgicl-ass_franssen_2407
SEQUENCE=AAAAGAATGTGTTTGGTAAGAAAGAGGATTCAAGTTGGGAAGAAGAGAACAATAGTGGTTTTAGTTTGTGGAAATGGAGTCTTAGTGGTGTTGGTGCTATTTGTACTTTTGGTGTTGTTGCTGCTTCTAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATGGGACCCCGCGTACTCTGTCGTTTGTATTTACCTTTTACTTTGTTTTTTCTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.9765
PRIMER_LEFT_PENALTY=0.065960
PRIMER_RIGHT_PENALTY=4.910554
PRIMER_LEFT_SEQUENCE=TGGAGTCTTAGTGGTGTTGGTG
PRIMER_RIGHT_SEQUENCE=GGTAAATACAAACGACAGAGTACG
PRIMER_LEFT=74,22
PRIMER_RIGHT=218,24
PRIMER_LEFT_TM=60.066
PRIMER_RIGHT_TM=57.089
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=5.0478
PRIMER_LEFT_1_PENALTY=0.065960
PRIMER_RIGHT_1_PENALTY=4.981798
PRIMER_LEFT_1_SEQUENCE=TGGAGTCTTAGTGGTGTTGGTG
PRIMER_RIGHT_1_SEQUENCE=AGGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=219,25
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=58.018
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=5.0604
PRIMER_LEFT_2_PENALTY=0.149895
PRIMER_RIGHT_2_PENALTY=4.910554
PRIMER_LEFT_2_SEQUENCE=TACTTTTGGTGTTGTTGCTGCT
PRIMER_RIGHT_2_SEQUENCE=GGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=218,24
PRIMER_LEFT_2_TM=59.850
PRIMER_RIGHT_2_TM=57.089
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=5.0979
PRIMER_LEFT_3_PENALTY=0.187326
PRIMER_RIGHT_3_PENALTY=4.910554
PRIMER_LEFT_3_SEQUENCE=TGGTGTTGTTGCTGCTTCTAAT
PRIMER_RIGHT_3_SEQUENCE=GGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_3=109,22
PRIMER_RIGHT_3=218,24
PRIMER_LEFT_3_TM=59.813
PRIMER_RIGHT_3_TM=57.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=5.1317
PRIMER_LEFT_4_PENALTY=0.149895
PRIMER_RIGHT_4_PENALTY=4.981798
PRIMER_LEFT_4_SEQUENCE=TACTTTTGGTGTTGTTGCTGCT
PRIMER_RIGHT_4_SEQUENCE=AGGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_4=103,22
PRIMER_RIGHT_4=219,25
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=58.018
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_00192 Ps_contig_mira-and-tgicl-ass_franssen_2408
SEQUENCE=TATGTGATTCGAGAGTGAGAATGCTCAATGAATCGAGATAGAGGGTGTGNGAAAGATGTGTTTGGTAAGAAAGAGGATTCAATTGGAAGAAGAGAACAATAGTGGTTTTAGTTTGTTGGTAAGTAGGAGKYGTTAGTTGGTTGGTTTGGGTTGGTCGTATTTWGTTTACGTTTTGGTTTGGTTTGGTTTGGTTCGTGTCGTTCTTCTTTNTTGTTTGTTGTTGTTTGTTAGTACGACAACGAAGAAA
TARGET=172,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00193 Ps_contig_mira-and-tgicl-ass_franssen_2422
SEQUENCE=GATGTTTTTCGTTGAATCTTCACAGAGAAAGTAAATTAGAGAGAGAGAGTAGAAAAGCTTGAAGCTGTATAAGAGACATCAGTGTCTCTAGTAGAGKGTGTTTGTCTTGTGAGAGAGAGAAAGTAAAGTGAATAGTTGGTGAAGATGATGAATAACTCAGTWAGTTTACAAGACAACTAGTTTTATTATTGAAAGTGAAGAAGAAGNTGATAAGGTATTTAACGATAGGAATTGTTTCTGTATTTCCTTTACTTAAAAA
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00194 Ps_contig_mira-and-tgicl-ass_franssen_2422
SEQUENCE=GATGTTTTTCGTTGAATCTTCACAGAGAAAGTAAATTAGAGAGAGAGAGTAGAAAAGCTTGAAGCTGTATAAGAGACATCAGTGTCTCTAGTAGAGKGTGTTTGTCTTGTGAGAGAGAGAAAGTAAAGTGAATAGTTGGTGAAGATGATGAATAACTCAGTWAGTTTACAAGACAACTAGTTTTATTATTGAAAGTGAAGAAGAAGNTGATAAGGTATTTAACGATAGGAATTGTTTCTGTATTTCCTTTACTTAAAAA
TARGET=111,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00195 Ps_contig_mira-and-tgicl-ass_franssen_2428
SEQUENCE=CATCATCATCATCTTCTTCCTCCTCTTCATCTTCTTCATCCACTGAAACAGGTTTGCGAGGACCTTCCGCGTAAATCTCAGGATCGTGAACAATCAAAAGCTTGAAGCCCATTT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00196 Ps_contig_mira-and-tgicl-ass_franssen_2452
SEQUENCE=TTTACCCGGTTGTCCTTTCCACCATACGGTATTGTACACCCGTACTTAATTTAACATAGAAAGAAGAGAAAAAAACAAACATTAGATTAGATCTAATCACTGTCGCTGCTTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCCATGGATCTTGTTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTYGTTACTTCCTCTTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTKKCAAMGGWAAMCCCTTTCTTTTCCTCAGTTACCGGTTTGTTCCTTCAAACAACCTCAACTTTCNCAACTGGCACCGAAGTNTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTCNCACCTATTTTCTCCTTCCTCTTCACCTTCCCGCGTACTCTGCGTTNAA
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00197 Ps_contig_mira-and-tgicl-ass_franssen_2452
SEQUENCE=TTTACCCGGTTGTCCTTTCCACCATACGGTATTGTACACCCGTACTTAATTTAACATAGAAAGAAGAGAAAAAAACAAACATTAGATTAGATCTAATCACTGTCGCTGCTTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCCATGGATCTTGTTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTYGTTACTTCCTCTTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTKKCAAMGGWAAMCCCTTTCTTTTCCTCAGTTACCGGTTTGTTCCTTCAAACAACCTCAACTTTCNCAACTGGCACCGAAGTNTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTCNCACCTATTTTCTCCTTCCTCTTCACCTTCCCGCGTACTCTGCGTTNAA
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00198 Ps_contig_mira-and-tgicl-ass_franssen_2452
SEQUENCE=TTTACCCGGTTGTCCTTTCCACCATACGGTATTGTACACCCGTACTTAATTTAACATAGAAAGAAGAGAAAAAAACAAACATTAGATTAGATCTAATCACTGTCGCTGCTTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCCATGGATCTTGTTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTYGTTACTTCCTCTTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTKKCAAMGGWAAMCCCTTTCTTTTCCTCAGTTACCGGTTTGTTCCTTCAAACAACCTCAACTTTCNCAACTGGCACCGAAGTNTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTCNCACCTATTTTCTCCTTCCTCTTCACCTTCCCGCGTACTCTGCGTTNAA
TARGET=514,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00199 Ps_contig_mira-and-tgicl-ass_franssen_2459
SEQUENCE=GTGGTATCAACGCAGAGTGTTATTTATTGTTTCTTTTACATTGTTATTCTTTTTGTTGTCCTTATTATTAGTATATAAATAAATAAACATGTACTTTTTCATCTGAGTTTTGGGATTCATGGAGGTCTTTGTTTTCTGAATAATAAATATTTAAAGTAACTAC
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3469
PRIMER_LEFT_PENALTY=2.159969
PRIMER_RIGHT_PENALTY=0.186949
PRIMER_LEFT_SEQUENCE=GTGGTATCAACGCAGAGTGTTA
PRIMER_RIGHT_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT=0,22
PRIMER_RIGHT=121,22
PRIMER_LEFT_TM=57.840
PRIMER_RIGHT_TM=60.187
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=2.6861
PRIMER_LEFT_1_PENALTY=2.499147
PRIMER_RIGHT_1_PENALTY=0.186949
PRIMER_LEFT_1_SEQUENCE=GTGGTATCAACGCAGAGTGTTATT
PRIMER_RIGHT_1_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT_1=0,24
PRIMER_RIGHT_1=121,22
PRIMER_LEFT_1_TM=59.501
PRIMER_RIGHT_1_TM=60.187
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=2.7308
PRIMER_LEFT_2_PENALTY=2.159969
PRIMER_RIGHT_2_PENALTY=0.570821
PRIMER_LEFT_2_SEQUENCE=GTGGTATCAACGCAGAGTGTTA
PRIMER_RIGHT_2_SEQUENCE=CATGAATCCCAAAACTCAGATG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=120,22
PRIMER_LEFT_2_TM=57.840
PRIMER_RIGHT_2_TM=59.429
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=121
PRIMER_PAIR_PENALTY_3=2.8301
PRIMER_LEFT_3_PENALTY=2.159969
PRIMER_RIGHT_3_PENALTY=0.670093
PRIMER_LEFT_3_SEQUENCE=GTGGTATCAACGCAGAGTGTTA
PRIMER_RIGHT_3_SEQUENCE=AAACAAAGACCTCCATGAATCC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=133,22
PRIMER_LEFT_3_TM=57.840
PRIMER_RIGHT_3_TM=59.330
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=2.8548
PRIMER_LEFT_4_PENALTY=2.667901
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=TGGTATCAACGCAGAGTGTTAT
PRIMER_RIGHT_4_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=121,22
PRIMER_LEFT_4_TM=57.332
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_00200 Ps_contig_mira-and-tgicl-ass_franssen_2461
SEQUENCE=TTTGTTGGCTTACCCAAAACGGGAAACGTAGAAAGACTTGGGTTTAGGGGAAAACGTTAAAGTAGGGAAGGGCGTAAAATTTACTTAGGGAAAGAACAGAACAAGCCCTCTACCTGTGGAAATAAATACAAACAAACCAAAAGGACTTTTTTAGGTTCTTACAAAAACCTAACAATTCTATTGGATTCTTGGAGCTGGCCATTTTGTACCTACTGATCAACCTTGTGTTGCATTAGAAATGATAGCTTCAATTACACAGTCACCAGCTGCTTGAAAAAGAAAACATTAGTACTATCTGTAGAATAAATTAAAGAAAAAAAGTCCAGGAAACAAAATTCATTGTGGATCAGGGAAAGAACAGCAATAAAGTCATGGAAAAGTGTTATTTATTGTTTCTTTTACATTGTTATTCTTTTTTGTTGTCCTTATTATTAGTATATAAATAAATAAACATGTACTTTTTCATCTGAGTTTTGGGATTCATGGGAGGTTCTTTTGTTTTCTGAAATAATAAAAATATTTAAAGTAACAAAAACG
TARGET=439,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2243
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.186949
PRIMER_LEFT_SEQUENCE=CTGATCAACCTTGTGTTGCATT
PRIMER_RIGHT_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT=212,22
PRIMER_RIGHT=485,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.187
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.4276
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.390220
PRIMER_LEFT_1_SEQUENCE=CTGATCAACCTTGTGTTGCATT
PRIMER_RIGHT_1_SEQUENCE=TCAGAAAACAAAAGAACCTCCC
PRIMER_LEFT_1=212,22
PRIMER_RIGHT_1=505,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.610
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.5232
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.485895
PRIMER_LEFT_2_SEQUENCE=CTGATCAACCTTGTGTTGCATT
PRIMER_RIGHT_2_SEQUENCE=CAGAAAACAAAAGAACCTCCCA
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=504,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=60.486
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.5772
PRIMER_LEFT_3_PENALTY=0.186949
PRIMER_RIGHT_3_PENALTY=0.390220
PRIMER_LEFT_3_SEQUENCE=ATTCATTGTGGATCAGGGAAAG
PRIMER_RIGHT_3_SEQUENCE=TCAGAAAACAAAAGAACCTCCC
PRIMER_LEFT_3=334,22
PRIMER_RIGHT_3=505,22
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=59.610
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.5815
PRIMER_LEFT_4_PENALTY=0.394568
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=CCAGCTGCTTGAAAAAGAAAAC
PRIMER_RIGHT_4_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT_4=262,22
PRIMER_RIGHT_4=485,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_00201 Ps_contig_mira-and-tgicl-ass_franssen_2467
SEQUENCE=GKMRKRKTCCGAGTATATAGATAACCAGAGGCAGATTTTAGTGATKGGAGATATTAGGGTTTAGAGAAACCACCAGCGGAGAAGCAGCCACCAGCGGAGAAGCAACAATGGTTCTCAAGACTGAACTCTGCCGATTCAGTGGTGCAAAGATCTACCCAGGAAGAGGCATCAGATTTATTCGCAGCGATTCTCAGGTGTTCCTATTTGTGAACTCAAAATGTAAGAGGTATTTCCACAACCGTTTGAAGCCTTCAAAGCTCACATGGACTGCCATGTATAGAAAGCAACACAAGAAGGACATTGCTCAAGAAGCTGTAAAGAAGAGACGTCGTGCTACCAAGAAGCCATACTCTAGGTCAATTGTTGGTGCTACCCTGGAAGTCATTCAGAAGAAAAGAGCCGAGAAGCCTGAAGTTCGAGATGCAGCTAGGGAGGCTGCTCTGCGTGAAATCAAAGAGAGGATTAAGAAAACAAAGGATGAGAAGAAAGCCAAGAAAGCAGAAGTAGCATCTAAGGCACAAAAGTCAGGCAAAGGCAATGTTCAGAAAGGAGGTTTGCCCAAGGGTCCTAAATTGGGCGGCGGCGGCGGTAAACGTTAAGTAGTTGCAATTTTGGCTGTAATTTGAATACACTTGTTTTTAAACTTCTATATTTATATTTTATTTGCTCGATTAGCTGACCTTATTATCACTCGTGTTTGGAGCTTCAGACACTACCTTATTGCTGAATGCATTTTACTAGCAATTCTATATTTTGTCTGCTAATAATTTGCATTGTTTAAATGGTTGTCTGTCTACGAGTCTCATAGCTCAAACATGCTTTTTATTTCTAATGTTGC
TARGET=576,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00202 Ps_contig_mira-and-tgicl-ass_franssen_2472
SEQUENCE=TTACCCCTAAAAACCCCTTACGGGTAACCGTTTTAAAAAAACGTTAAAAATTTTTTAAAAAACGGTTTTAAGGGTTACCCTAAGGGGTAACCCCCGAACCGAAAACCCCTTAAAAAACCCAAACCTAATTCCCTAAACGACTCCAATCATGATATAGATCGAAGCATATGCAAATTTATTAAGCCAAAACAGAGCACCTTCTCTCCCACATAAAGCTCCATTGCCTTCTCTTGAAAACTCATTTCCTCCCATTTCTTAGGAGGAGCATCTGCCTTACTGGGTTGCTCTATGCTATTTGTAACAGTTCCTTTCTCCCTCTGCCGTTTCGCTTGTCTCCTTGTTCTTCTCCTGAGCCTTAGCTCAACCAGTTTAGCCGGTGTTGTAGTTGGTGCTTGAATTGAGTTTGTTTCATCCTGGTCTGTTTCGGCAGCGTTGCTGTTGCTGCTGCTGCTGCATGTGATGGAATAGAAAAGGGAGAGAAGT
TARGET=440,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8841
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=1.812450
PRIMER_LEFT_SEQUENCE=GTTTCGCTTGTCTCCTTGTTCT
PRIMER_RIGHT_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT=322,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=58.188
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=1.9350
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=1.812450
PRIMER_LEFT_1_SEQUENCE=TTAGGAGGAGCATCTGCCTTAC
PRIMER_RIGHT_1_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_1=255,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=58.188
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=1.9428
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=1.812450
PRIMER_LEFT_2_SEQUENCE=AGCACCTTCTCTCCCACATAAA
PRIMER_RIGHT_2_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=476,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=58.188
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=1.9428
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=1.812450
PRIMER_LEFT_3_SEQUENCE=GCACCTTCTCTCCCACATAAAG
PRIMER_RIGHT_3_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_3=193,22
PRIMER_RIGHT_3=476,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=58.188
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=1.9428
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=1.812450
PRIMER_LEFT_4_SEQUENCE=CACCTTCTCTCCCACATAAAGC
PRIMER_RIGHT_4_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_4=194,22
PRIMER_RIGHT_4=476,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=58.188
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_00203 Ps_contig_mira-and-tgicl-ass_franssen_2483
SEQUENCE=TACGTCGGCGGATGGGGGGATCTGTGTCAATCCCAACATAGTATTCATCTATGGCAGCAACAATGGCAATGATGATCAACTCCAAGTGTCTAAACACAGCCATACCCAAGTTACAAAACACNTCTACCACAGCGAAACTAACAACAACAACATGCAAAAACCCACCACCGTTACAAAACCTAACCAAGGCTATTACCTCATTTGACAACACCACGTTGTTGTGTCACCTTCCATTGCAATT
TARGET=140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3928
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.312496
PRIMER_LEFT_SEQUENCE=CACAGCCATACCCAAGTTACAA
PRIMER_RIGHT_SEQUENCE=CAATGGAAGGTGACACAACAAC
PRIMER_LEFT=94,22
PRIMER_RIGHT=235,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.312
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=0.4066
PRIMER_LEFT_1_PENALTY=0.339170
PRIMER_RIGHT_1_PENALTY=0.067419
PRIMER_LEFT_1_SEQUENCE=CAATGGCAATGATGATCAACTC
PRIMER_RIGHT_1_SEQUENCE=GCCTTGGTTAGGTTTTGTAACG
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=189,22
PRIMER_LEFT_1_TM=60.339
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.4157
PRIMER_LEFT_2_PENALTY=0.103216
PRIMER_RIGHT_2_PENALTY=0.312496
PRIMER_LEFT_2_SEQUENCE=AACACNTCTACCACAGCGAAAC
PRIMER_RIGHT_2_SEQUENCE=CAATGGAAGGTGACACAACAAC
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=235,22
PRIMER_LEFT_2_TM=59.897
PRIMER_RIGHT_2_TM=60.312
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.5368
PRIMER_LEFT_3_PENALTY=0.339170
PRIMER_RIGHT_3_PENALTY=0.197640
PRIMER_LEFT_3_SEQUENCE=CAATGGCAATGATGATCAACTC
PRIMER_RIGHT_3_SEQUENCE=CCTTGGTTAGGTTTTGTAACGG
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=59.802
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.6174
PRIMER_LEFT_4_PENALTY=0.419726
PRIMER_RIGHT_4_PENALTY=0.197640
PRIMER_LEFT_4_SEQUENCE=TGTCTAAACACAGCCATACCCA
PRIMER_RIGHT_4_SEQUENCE=CCTTGGTTAGGTTTTGTAACGG
PRIMER_LEFT_4=86,22
PRIMER_RIGHT_4=188,22
PRIMER_LEFT_4_TM=60.420
PRIMER_RIGHT_4_TM=59.802
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_00204 Ps_contig_mira-and-tgicl-ass_franssen_2485
SEQUENCE=GTTCGTTCGGGTTTATWGATCACTTGCAATCTTCTCTTTCTCATTTTCAATCATTCTTCCAGAAACCCTTTTCTTTCGTTTCAATCTTTTCATCTGCCATGGCGCCCAAGGGAGAGAAGAAACCAGCGGCAAAGAAGCCTGCAGAGAAGGCACCAGTAGAACCCAAGGCTGAGAAAAAGATCCCGAAGGATGCTTCCTCCACCGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAGACGTACAAGATCTACATCTTCAAGGTTCTTAAGCAAGTTCATCCTGACATCGGAATCTCCAGCAAAGCTATGGGGATCATGAATAGCTTCATCAATGATATCTTCGAGAAGCTCGCTCAGGAGTCTTCTAGACTCTCTCGCTACAACAAAAAATCCACCATCTCTTCCCGGGAGATCCAGACTGCTGTTCGTCTTGTTCTTCCCGGTGAGCTTGCCAAGCACGCCGTTTCCGAGGGAACCAAATCTGTCACCAAGTTCACCAGTTCTGATTAGAAATACTGATTTAAATTAGGGTTTACTTGATGTATATTTTGGATTTTCTCGTTTTCGTTTTAGTAAAGAAATATCTCTAATGAAATTAATTTTTGGGAC
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00205 Ps_contig_mira-and-tgicl-ass_franssen_2487
SEQUENCE=GTCGGGGGATCGCTTTCTCATTCTCGATTTTTCTTCCAGAAACCCTTTTCTTTCGATCAAATCTTTTCATCATCCATGGCCCCTAAGGGAGAGAAAAAACCAGCGGAGAAGAAGCCCGTAGAGAAACCTAAGGCTGAGAAAAAGATCCCGAAGGACGTTTCCTCCACCGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAGACGTACAAGATCTACATCTTCAAGGTTCTTAAGCAAGTTCATCCTGACATTGGAATCTCCAGCAAAGCTAATGGGGATCATGAAAACAGCTTCATCAATGATATCTTCGAGAAGCTCGCTCAGGAGT
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3171
PRIMER_LEFT_PENALTY=0.093130
PRIMER_RIGHT_PENALTY=0.224014
PRIMER_LEFT_SEQUENCE=CCGTAGAGAAACCTAAGGCTGA
PRIMER_RIGHT_SEQUENCE=CCATTAGCTTTGCTGGAGATTC
PRIMER_LEFT=115,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.224
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.3980
PRIMER_LEFT_1_PENALTY=0.174021
PRIMER_RIGHT_1_PENALTY=0.224014
PRIMER_LEFT_1_SEQUENCE=GATCGCTTTCTCATTCTCGATT
PRIMER_RIGHT_1_SEQUENCE=CCATTAGCTTTGCTGGAGATTC
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=59.826
PRIMER_RIGHT_1_TM=60.224
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.4206
PRIMER_LEFT_2_PENALTY=0.093130
PRIMER_RIGHT_2_PENALTY=0.327468
PRIMER_LEFT_2_SEQUENCE=CCGTAGAGAAACCTAAGGCTGA
PRIMER_RIGHT_2_SEQUENCE=ATGAAGCTGTTTTCATGATCCC
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.327
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.4207
PRIMER_LEFT_3_PENALTY=0.093130
PRIMER_RIGHT_3_PENALTY=0.327533
PRIMER_LEFT_3_SEQUENCE=CCGTAGAGAAACCTAAGGCTGA
PRIMER_RIGHT_3_SEQUENCE=CTGGAGATTCCAATGTCAGGAT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=265,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=60.328
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.4784
PRIMER_LEFT_4_PENALTY=0.254402
PRIMER_RIGHT_4_PENALTY=0.224014
PRIMER_LEFT_4_SEQUENCE=AGAAGAAGCCCGTAGAGAAACC
PRIMER_RIGHT_4_SEQUENCE=CCATTAGCTTTGCTGGAGATTC
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=60.224
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_00206 Ps_contig_mira-and-tgicl-ass_franssen_2506
SEQUENCE=TGGTTTGAAACGTTTCCGGTCAACTCCGGCGAGCTGGATAGAATCGTTTATTCTCAAAGAAGAAGAAGAGGAGGACGAAAACCAACAACAAGACAATTTCAGTTTCACTCAACTGCTTTCCAATAACGCTGCTGCCGGTCCTTCAACTCCCGATTCTCATCCATATTTACCTGATTATTACCAGTACTCTTCACCGCATACTCTTTCTGCCGCAGATGCCTCTAATAATCCCTTCACACAGCCAAAAAGTATTCAAATATTCAAGACAAAATGAGACAGAAGGAGGATTGACTATACGAAAAGATAATCAAATAGTTACAGAAAGATGATATGAATTAAATTGTGTATATACTGATTTGGAGTATTATAACATAGACATTATGAGGTTGTTTGATTTGTATAAACAAACCACTGCTTAGGGGTTGAGGAGAGTGATAATGCATTGGATCTTAAAATAGACAAAATTTTAGAGGATTCGGTTCCTTGCAAGATTCGGGCAAAGCGTGGCTGTGCTACACATCCAAGGAGTATTGCTGAAAGGGTAACTGTTAATACTAATTTGGGATTTAGACTTAATCCGAATTGTGTGAATCTTTTCTGTTTT
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1934
PRIMER_LEFT_PENALTY=0.710129
PRIMER_RIGHT_PENALTY=0.483253
PRIMER_LEFT_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_SEQUENCE=CAGAAAGAGTATGCGGTGAAGA
PRIMER_LEFT=28,22
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=60.710
PRIMER_RIGHT_TM=59.517
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=1.2362
PRIMER_LEFT_1_PENALTY=0.710129
PRIMER_RIGHT_1_PENALTY=0.526059
PRIMER_LEFT_1_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_1_SEQUENCE=TGGCTGTGTGAAGGGATTATTA
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=60.710
PRIMER_RIGHT_1_TM=59.474
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=5.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=216
PRIMER_PAIR_PENALTY_2=1.4178
PRIMER_LEFT_2_PENALTY=0.710129
PRIMER_RIGHT_2_PENALTY=0.707667
PRIMER_LEFT_2_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_2_SEQUENCE=AAATATGGATGAGAATCGGGAG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=167,22
PRIMER_LEFT_2_TM=60.710
PRIMER_RIGHT_2_TM=59.292
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=1.4316
PRIMER_LEFT_3_PENALTY=0.710129
PRIMER_RIGHT_3_PENALTY=0.721451
PRIMER_LEFT_3_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_3_SEQUENCE=TTGGCTGTGTGAAGGGATTATT
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=60.710
PRIMER_RIGHT_3_TM=60.721
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=1.4316
PRIMER_LEFT_4_PENALTY=0.710129
PRIMER_RIGHT_4_PENALTY=0.721451
PRIMER_LEFT_4_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_4_SEQUENCE=TTTGGCTGTGTGAAGGGATTAT
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=60.710
PRIMER_RIGHT_4_TM=60.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_00207 Ps_contig_mira-and-tgicl-ass_franssen_2511
SEQUENCE=AGCAAATATCATTCCTACATGAGCAACAATTCGTCTCTCTCTCTAACCCAGAGCAACAATATGCTCTGCAACTTCCGACCCAACCCACAGCCAAATCCCACTGGAGAAGACGGTGGCGGCCGGCCAACGGCGGCGGCGCCACCTTCTTCTCCGGCGGACCTCTACCTCACAACTTAAATTCCAAACACTAACCCTACCTAGTCTAACCTCACGATCACAAATCCGGTCACGTAAACACCAAAAGGTTAACCTAAAGCCTTGGATCGGAAAACCCTAACCATACACAAAAAATCTAAACAAAGTTCTTTACAAACCAAACCATATAAATCTTCTCCTCAAGCCAAAACAAACACAAAAGCAAATGAACACTAACATTCAGAGTCAAAACCCTAAGTCTACAAATGAAGCCACAACGGCGGAGATTTTAAGCAATTGACATGGGGGTTTTGGTCGGCTTATCAAGACGAATGAGTTGGTGCAAATATTTAAAGCGAAAGAGAAAGAAGAAGTACTTGACCGGAAGTGGGTCGCCGACAACGTCTTCTCCGCGGCCGTTTTCAGATAAGCTCCTTTCTAATAAACCAACTCCTTTCGTCTTACTCTGTTCTTTCTTAACTTTGAGCTCCTTTAGATGGTTGTGTTGATGAAATATTATTTTGATTTCTTTTTCGTTTCTTGATTCACTCCCCTTTTCAATGTCCTCTGTTCTGGGTATTTATGTTCTTTTCATGATGAATTTATTCAGTCTTGAGATAACTTGTTAGGTTTCTTTTTTCTTATCCCCAGATTTCATGGGATTGTTGTTTTGTGGTTTCATTGTTGTTTGAACTGGTTAACGGAACGCATTCTTGGGTGTTCATGGTAGTCTTTTAGGCAAAACATTAACCTAAGCGTTTTGTTAATTATCCTTCTAATTTTTTAAGCGCTTATTAGAAAAAGTACTTTTAACTTAGATTGTCAACTTCCTTCGNAGGTCAACAAAATCAATTGAAGGAAAAAAGGAACGNTNTNTATTGTTCTAAGAAAGGAGGGTTTGTAGTAA
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2398
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_SEQUENCE=GTGTATGGTTAGGGTTTTCCGA
PRIMER_LEFT=11,22
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.5218
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=0.390906
PRIMER_LEFT_1_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_1_SEQUENCE=TGTATGGTTAGGGTTTTCCGAT
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=1.1054
PRIMER_LEFT_2_PENALTY=0.130883
PRIMER_RIGHT_2_PENALTY=0.974470
PRIMER_LEFT_2_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_2_SEQUENCE=TGTGTATGGTTAGGGTTTTCCG
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=285,22
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=60.974
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=1.3090
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=1.178157
PRIMER_LEFT_3_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_3_SEQUENCE=GTTAGGGTTTTCCGATCCAAG
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=277,21
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.822
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=1.3424
PRIMER_LEFT_4_PENALTY=0.130883
PRIMER_RIGHT_4_PENALTY=1.211563
PRIMER_LEFT_4_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_4_SEQUENCE=TATGGTTAGGGTTTTCCGATCC
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.131
PRIMER_RIGHT_4_TM=61.212
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_00208 Ps_contig_mira-and-tgicl-ass_franssen_2512
SEQUENCE=GTGAGMAAAKATCATTCCTATATGAGCAACAATTCGTCTCTCTCTCTAACCCAGAGCAACAATATGCTCTGCAACTTCCGACCCAACCCACGGCCAAATCCCACTGGAGAAGACGGTGGCGGCCGGTCAACGGCGGCGGCGCCGCCTTCTTCTCCGGTGAACCTCTACCTCACAACTTAAATTCCAAACACTAACCCTACCTATTCTAGCCTCACGATCACAAATCCGGTCACGAAAACACCAAAAGGTTAACCTAAAGCCTTGGATCGGAAAACCCTAACCATACACAAAAATCTAAACAAAGTTCTTTACAAACCAAATCATATAAATCTTCTCCTCAAGCCAAAACAAACACAAAAGCAAATGAACACTAACATTCAGAGTCAAAACCCTAAGTCTATAAATGAAGCCACAACGGCGGAGATTTTAAGCAATTGACATGGGGTTTTTGGTCGGCTTATCAAGACGAACGAGGTGGTGCAAATATTTAAAGCGAAAGAGAAAGAAGAAGTACTTGACCGGAAGTGGGTCGCCGACAACGTCTTCTCCGCGGCCGTTTTCAGATAAGCTCCTTTCTAATAATCCAACTCCCTTCGTCTTACTCTGTTATTTTTTAACTTTGAGCTCCTTTAGATGGTTGTGTTGATGAAAGATTATTTTGATTTCTCTTTCGTTTCTTCCTTCACTCTCCTTTTCAATGTCCTCTGTTCTGGGTATTTATGTTCTTTTCATGATGAATTTATTCAGTCTTGAGATAACTTGTTAGGTTTCTTTTTTTCTTATCCCCAGATTTCATGGGATTGTTGTTTTGTGGTTTCATTGTTGTTTGAACTGGTTAACGGAATGCATTCTTGGGTGTTCGGGGTAGTCTTTTAGGCAAAACATTAACCTGAGCGTTTTGTTAATTATCCTTCAGTTTTTAAGCGCTTATTGAAAAAGCTTTTAACTTAGATTGTCACTTCCTTCGCAGGTCACAAAATCAATTGAGGAAAAAAGGAGCGGATGTATGTTGGTTCTAAGAAGAGGTTGGGATTTTTAGAGTGCTTTGGCCCAAGAGATGACGTTGGCCGCTGGCTTTTTGGAAATTCCATCTTCACACTGCTGGACAATGCAGCAACCTTTAATTTTCAAAATTAGTCATTTAAGATGTACTTTGGACTTTTGGAACGTATTGGATAATGTATCAGCCTTTGGATAATTATGTAACTTTGGGAATTTTGTAACATTTTGGATTAGAATTCATTTCCGTAATTAATCCTTTTTAATCATTCHAAACTTGACYTAG
TARGET=37,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00209 Ps_contig_mira-and-tgicl-ass_franssen_2514
SEQUENCE=AAAAACAACAAAATAAACTACTTGGCTCAAAGCTTTACCCTTAACAGATTAACATGAATACTCAAAAAARCRCTTCGAAAATAACTTAAAACAGTAACACATTTACAAAAATGGAATCCACAATAACCGGGAATGGAAAGGCATAACAGACGGTTTTTCTTTTGAGGATAAAAAATTCATCTGTGAAGAGATCAGGAGATATATAAGAACACGTCTAAAAAATCCAGGGGGACGGAGATACTTCTTTGTAAGCATAAAATCCAAAAAACAACACAACATACGTTTTGGGGAAATCTAAGAGAAGCAAWTAAAATTGTTTTTCTCTGTTAAACTCAAAAAATATTTCCTTAACACGAAGCCAGATTCAACAGTGAATCAAGAAACAAAAAATAATCTTTCATCAACACAACCATCTAAAGGAGCTCAAAGTTAAACAAGAACAGAGTAAGACGAAGGGAGTTGGATTATTAGAAAGGAGCTTATTTGGAAACAACCGCGGAGAAGACGTCGCCGGCGACCCACTTCCGGTCAAGTAATTCTTCTTTCTCCTTCGCTTTAAATATTTGCACCACCTCGTTCGTCTTGATAAGCCGACCAAAACCCTCATGTCAATTGCTTAAAATCTCCGCTGTTGTGGCTTCATTTGTAGACTTAGGGTTTTGACTCTGAATGTTGGTGTTTATTTGCTTTTGTGTTTGTTTTGGCTTGAGGAGAAGATTTATGTGGTTTGGTTTGTAAAGAACTTTGTTTAGATCTTTGTGTGTGGTTGGGGTTTTCCGATCCAAGGCTTTAGGTTAACCTTTTGGTGTTTTCGTGACCGGATTTGTGATCGTGAGGTTAGAATAGGTAGGGTTAGTGTTTGGAATTTAAGCTGTGAGGTAGAGGTTTGTCGGAGAAGAAGGCGGCGCCGCCGCCGTTGGCTGGCCGCCACCGTCTTCTCCGGTGGGATTTGGTCGTGGGTTGGGTCGGAAGTTGCAGAGCATATTGTTGCTCTGGGTTAGAGAGAGAGAGACGAATTGTTGCTCATGTAGGAATGATATTTGCTCCCCCGAGTACT
TARGET=1000,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00210 Ps_contig_mira-and-tgicl-ass_franssen_2516
SEQUENCE=GATTCCTACATGAGTAACAAATCGTCTCTCTCTCTAACTCAGAGCAACAATGTGCTCTGCAACTTCCGACCCAACCCACAGCCAAATCCCACCGGAGAAAACGGTGGCGGCCGGTCAACGGTGGCGGCCGGTCAACGGTGGCGGCGGCGCCTTCTTCTTCGGCGAACCTCTACCTCAGAGCTTAAATTCCAAACACTAACCCTACCTATTCTAACCTCGCGATCACAGATCCGGTCACGAAAACAA
TARGET=25,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00211 Ps_contig_mira-and-tgicl-ass_franssen_2524
SEQUENCE=CCAAGAAGTGTGAGTTCAATTGGGGATTGTTTGTTATTTAGTTTGAAGGTTGGGAAGGTGAAGCTTATTGGGTTCATGTTTGGACATTGAAAAAATGGGTTTGATTACGCAATTTAGAGAGTATTTTAATACATGGCTTGTTGTGAGAGATTTGAGGCCATTGAGATGGGAAGAAGATCATCATCATCAATAAGCATGATAGTATGATGTACTAGTTTGGTAGGGAAGTAAACGGTTACCCTAAACCCGTTCGGTTAGTTAACTTTTCCGTTTTTAACGGTTCCGGGTTTAAACGGGGTTTACCCCGTTTAAACCCCGGGTTTTTTACCCCCGGGGGGGTTTAAACCGTTTTTTGGGGGTTTTACGGGGTTTTTTAACCGGGGGTTTAAACCCTTTTTTAAAAAACGGTTTTTTTAAAAAACCCGGGAAAAAAACCCCTTAAAACCGGTTAAAAAGGTTTAAAACCGGGTAAAAAACCCGTTTTAAAAAGGGTTTTTTTAGGGGGTTTTTTTTAACGGGGTTTTAACCGGGTTTTTTACCCGGTTTTTTAAGGGGTTTTTACGGTTTTTTTTTAAACCCGG
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5520
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.551271
PRIMER_LEFT_SEQUENCE=GTTGGGAAGGTGAAGCTTATTG
PRIMER_RIGHT_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT=48,22
PRIMER_RIGHT=260,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.449
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.6468
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.551271
PRIMER_LEFT_1_SEQUENCE=GTGAGTTCAATTGGGGATTGTT
PRIMER_RIGHT_1_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.449
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.7880
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.551271
PRIMER_LEFT_2_SEQUENCE=AGCTTATTGGGTTCATGTTTGG
PRIMER_RIGHT_2_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.449
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.8163
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=0.551271
PRIMER_LEFT_3_SEQUENCE=AGTGTGAGTTCAATTGGGGATT
PRIMER_RIGHT_3_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=260,22
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=59.449
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.8163
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.551271
PRIMER_LEFT_4_SEQUENCE=AAGTGTGAGTTCAATTGGGGAT
PRIMER_RIGHT_4_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=260,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=59.449
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_00212 Ps_contig_mira-and-tgicl-ass_franssen_2525
SEQUENCE=CCGTTTTAACCTTTTAATTTTTACCTAACTTTACTTTTTTTTCCCTTTTCCCTAAAATTTAGGTTGATAACTTTACGCATAACCAATTAACCACAAGACATGAAAAGGATGTACACAACACATTACATTAAAACAACCAAATAAAACACAAGACACAAACATTGTCATGTGTTCTAAATAGCCAACACAAGTCCCGGAAGTGACCTACGATAAAGATCACGAGGTTGACTTCTCCACAATGTCATCATACTATCATGCTTATGATGATGATGATCTTCTTCCCATCTCAATGGCCTCAAATCTCTCACAACAAGCCATGTATTAAAATACTCTCTAAATTGCGTAATCAACCCATTTTTCAATGTCCAAACATGAACCCAATAAGCTTCACCTTCCCAACCTTCAACTATAACACAATCCCCAATTGAACTCACACTTCTTGGCTCAAATTTGAACCCTTTTTTGTGATCTATCTCACCCGTTAACACCTTCATCATGTGTTGACATTTTGGTGGACCATGGAACCAATACTCGAGATCACTTGCTAGCAATTTTGATACCGTTTGGAATTCCATTGCTCCTTGGCCTAGTAATGCTTTGTAGAGAGTCTCAACTATGGACTTGTTTTGTTTTTCAATCATTGAGTCCATTTTAGAGAGAATGGTTACTTTGTTGAGTTACCACTCCCCGACTACTCTGCGTTG
TARGET=261,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0155
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_SEQUENCE=GTTGGGAAGGTGAAGCTTATTG
PRIMER_LEFT=193,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.1103
PRIMER_LEFT_1_PENALTY=0.014720
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_1_SEQUENCE=GAGTTCAATTGGGGATTGTGTT
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=431,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.2299
PRIMER_LEFT_2_PENALTY=0.014720
PRIMER_RIGHT_2_PENALTY=0.215215
PRIMER_LEFT_2_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_2_SEQUENCE=CAAATTTGAGCCAAGAAGTGTG
PRIMER_LEFT_2=193,22
PRIMER_RIGHT_2=452,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=59.785
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.2514
PRIMER_LEFT_3_PENALTY=0.014720
PRIMER_RIGHT_3_PENALTY=0.236697
PRIMER_LEFT_3_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_3_SEQUENCE=AGCTTATTGGGTTCATGTTTGG
PRIMER_LEFT_3=193,22
PRIMER_RIGHT_3=386,22
PRIMER_LEFT_3_TM=60.015
PRIMER_RIGHT_3_TM=60.237
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.2555
PRIMER_LEFT_4_PENALTY=0.254773
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=AGATCACGAGGTTGACTTCTCC
PRIMER_RIGHT_4_SEQUENCE=GTTGGGAAGGTGAAGCTTATTG
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=399,22
PRIMER_LEFT_4_TM=59.745
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00213 Ps_contig_mira-and-tgicl-ass_franssen_2527
SEQUENCE=GATCTATCCAACTTTACCACTTACTAATTTATTTATTCACAACATCATGAGTTKAGAAATTGGCAGCGGAATCATCGGAAGGTGRAGAAGAAGGAAAGYTAAGCGGCGGCAACCAGATATTGACTGTCGACGACGACCTCCGAGAATTGGGGAAAAAGGCCGCCTGGAGCGTCAGTTCCTGCAAACCCGGCAACRGCGTTTCTTCTCTTCGAGATGACAATCTCGAGACCTATTGGCAGTTACTCTCACTCACTCATCTTCTCATGCATTCTATTTTCATTAA
TARGET=245,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00214 Ps_contig_mira-and-tgicl-ass_franssen_2528
SEQUENCE=TAGTCTAACANACTTATCCTTACGTCTCACAAAACTACWATACCACAAAAGGACTTGTTTACTAATGTGCAAAAGACTTCAAAAATACTTAAACCATCAGAATATGCTGCTCTTCATGATTGTGCTGAAGAAATCAGTGATAGTGTTGATAGGCTTAGCCGTTCATTGAAAGAGCTTAAACTCTGCAAGATCAACGGTCAGGATTTCAATTGGCATATAAGCAATGTGGAGACATGGGTTAGCTCAGCTTTGACAGATGAGAGCACTTGTGGGGATGGATTTAGTGGAAAAGCACTTGATGGAAAAATCAAAGCTTCTATTAGATCTAGAATGGTTAATGTTGCTCAAGTTACTAGTAATGCTCTTTCACTTATTAATCAATATGCTGCTAAACACTAGGTAGAAATCAAACACTAGGAATCAAAGGTTTTGCTTTTAAGGTTGTTGAGAATGCCTTTAATGTTGTTGGTTCCTTTTTTATTTTGTTTTAATTTATGTGGTGTGTAATGTGTAATTACATCTATATCTATATATATAAAGAGATCACAAAACTTTGTCAAGTGTGAAATAAATTTTCTATTTATTTTTATTGTATTTTTTTTGTTTST
TARGET=528,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00215 Ps_contig_mira-and-tgicl-ass_franssen_2535
SEQUENCE=CCTAATTCACTCTTCTTCTTCTACCTTTCCAAACTTACTTGCATTAACATTCTCAAATTACAACACCAGAACTCCAATCCAATCCTCACCTTCTTCCACAAACATTTGCACCCAAAAAATACGACACATCTTTGATCACCATCGCCGAATCTTTCTACGATGATGAACTCTGGGCAGCTTCTTCCCTCCGTGTTCGTTCCTTCAATCAATTCCGTTCCCGGAACTTACGGAGTCCAATTTTCTACTTATGGAGAAGACAGGATAGTAGTTGGTTCTCTCGATCTTAATCAGTGCTTGAGTCTTCCGGATGAAATTGTAGGAATGAAGCCRGAGGTTAGCGGAGCTGATACGACAAGGGCTTACCTGAGTAATGTGTGTGTTGCTAGAGAGTTGCATAGGAATGGATTGGCCTATGAACTTCTTGAAAAGTCGAAGTTAGTTGCTCGTAATTGGGGCATAACAGATTTATACGTCCATGTTGCCGATGACAATGAACCAGACAAAAGAAATTGTACATAGGAAGTAGGTTGGGTTTTGTTTAACGGAAGG
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00216 Ps_contig_mira-and-tgicl-ass_franssen_2537
SEQUENCE=TTTWTTTACGGCTTAGAAAATAGAAAGGAAGGAAAGTAATGAGGCAATGAGGGAATGTTCTGAAAATAACTTAAAAAGAATAGACCATATTAACGCAGGATACCCGACAACACCGTCAAGTTAGGAGATTGAAAGACCCATCCATAGCAGAAGTCTTCGCGGTCGATCAAGAAACCCGGCCTGCCATGCAGGTTCATCACTCTCATAAACAAACCCGCTCTTCATGTACAATTTCTTTGCTGGTTCATTGTCATCGGCAACATGGACGTATAAATCTGTTATGCCCCAATTACGAGCAACTAACTTCGACTTTTCAAGAAGTTCATAGGCCAATCCATTCCTATGCAACTCTCTAGCAACACACACATTACTCAGGTAAGCCCTTGTCGTATCAGCTCCGCTAACTTGGACTCCGTAAGTTCCGGGACGGAATTGATTGAAGGAACGAACACGGAGGGAAGAAGCTGCCCAGAGTTCATCATCGTAGAAAGATTCGGCGATGGTGATCAAAGATGTGTCGATTTTTTGGGTGCAAATGTTTGTGGAAGAAGGTGAGGATTGGATTGGAGTTCTGGTGTTGTAATTTGAGAATGTTAATGCAGAATTTGGAAAGGTAGAAGAAGAAGAGTGAATTGAAGGGAAGGAACAGAGCGAAGAAATTAGCGTCATTTTTTGTTAGGTGTGTTGTTTGTTGTATTATTCGTCGTTGCATCCCCT
TARGET=616,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00217 Ps_contig_mira-and-tgicl-ass_franssen_2553
SEQUENCE=AGAGCTCCTTCGAATAAGCAGAATATTTTATTCATACCTTACACTGCAAATGATCTGGGATGCCACGGTGACTCCTCGTGGTGCGTGTAAGCCAACTGCACGTCGTCCCCAACCTGCAGCGACACCGCCGACGCCTCCTCTCACAACTTACTTACAAGTAAACGTAACGTCCGTCCGTCGTTCCGGTTACGGACGGTACGGTTCGGTTCCGGTACCGGTACCGGTCCGTCCGACCCAACCGACCGTCCGTTTCCCGGTTACCGGGAACGGGAACGGAACCGACGGACGGACGTACTACGTA
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6543
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=3.538580
PRIMER_LEFT_SEQUENCE=GAGCTCCTTCGAATAAGCAGAA
PRIMER_RIGHT_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT=1,22
PRIMER_RIGHT=203,20
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=61.539
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=60.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=3.7706
PRIMER_LEFT_1_PENALTY=0.232060
PRIMER_RIGHT_1_PENALTY=3.538580
PRIMER_LEFT_1_SEQUENCE=AGAGCTCCTTCGAATAAGCAGA
PRIMER_RIGHT_1_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=203,20
PRIMER_LEFT_1_TM=59.768
PRIMER_RIGHT_1_TM=61.539
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=3.9068
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=3.538580
PRIMER_LEFT_2_SEQUENCE=CTTACACTGCAAATGATCTGGG
PRIMER_RIGHT_2_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=203,20
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=61.539
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=3.9068
PRIMER_LEFT_3_PENALTY=0.368190
PRIMER_RIGHT_3_PENALTY=3.538580
PRIMER_LEFT_3_SEQUENCE=CCTTACACTGCAAATGATCTGG
PRIMER_RIGHT_3_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=203,20
PRIMER_LEFT_3_TM=59.632
PRIMER_RIGHT_3_TM=61.539
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=4.0404
PRIMER_LEFT_4_PENALTY=0.501869
PRIMER_RIGHT_4_PENALTY=3.538580
PRIMER_LEFT_4_SEQUENCE=TTACACTGCAAATGATCTGGGA
PRIMER_RIGHT_4_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=203,20
PRIMER_LEFT_4_TM=60.502
PRIMER_RIGHT_4_TM=61.539
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_00218 Ps_contig_mira-and-tgicl-ass_franssen_2553
SEQUENCE=AGAGCTCCTTCGAATAAGCAGAATATTTTATTCATACCTTACACTGCAAATGATCTGGGATGCCACGGTGACTCCTCGTGGTGCGTGTAAGCCAACTGCACGTCGTCCCCAACCTGCAGCGACACCGCCGACGCCTCCTCTCACAACTTACTTACAAGTAAACGTAACGTCCGTCCGTCGTTCCGGTTACGGACGGTACGGTTCGGTTCCGGTACCGGTACCGGTCCGTCCGACCCAACCGACCGTCCGTTTCCCGGTTACCGGGAACGGGAACGGAACCGACGGACGGACGTACTACGTA
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00219 Ps_contig_mira-and-tgicl-ass_franssen_2591
SEQUENCE=GAGTATAAATTTAGTTCTCTCGTATTCATTATTCATTTTCATTATTACGAATCTATCCATGGCTTCTTCTTTCACTTCGTTCCTTCGATTCAACAACCGNTGGATTTGATACTCTCAAGAACTTTGGTCAAATTTCTCCCGTTGTGAGGAATCATCTCAAACAGGTTTACTGACCTTGTGTTTGCGGTGCTGCTGCTGCTGTGGNTCTACT
TARGET=188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00220 Ps_contig_mira-and-tgicl-ass_franssen_2592
SEQUENCE=GGACTTTGATGAAGAAAGAAAATTACAACTACCAGACTTCATTAGGGTTTTCTCCCAGACTTCATCCKATYWKMATKTTATTTACGAATCTATCCATGGCTTCTTCTTTCACTTCGTTCTTCGATTCAACAAACCGATGGAATTTTGATAACTACTCAAGAACTTCGGTCAAATTTCTCCCGTTGTGAGGAATCATCYCAAACAGGTTTACTTGACCTTGTGTTTTGCGGTTGCTGCTGCTGCTGTTGGACGTCCTTTACCTTCATTCGTTCCCTTCTTTCCACCGTTGGG
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00221 Ps_contig_mira-and-tgicl-ass_franssen_2593
SEQUENCE=GACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAGGAATTGATCCAACAGGTATCCATTACCTGCGTGGATCTCTACTCCATCAAAACCTGCTTTCCATTGCATTTACCTTTTGCGGCAATTTTAAAATCATTGACAAT
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6728
PRIMER_LEFT_PENALTY=1.577079
PRIMER_RIGHT_PENALTY=0.095684
PRIMER_LEFT_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT=8,21
PRIMER_RIGHT=142,22
PRIMER_LEFT_TM=60.577
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=1.6728
PRIMER_LEFT_1_PENALTY=1.577079
PRIMER_RIGHT_1_PENALTY=0.095684
PRIMER_LEFT_1_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_1_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT_1=7,21
PRIMER_RIGHT_1=142,22
PRIMER_LEFT_1_TM=60.577
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=1.7017
PRIMER_LEFT_2_PENALTY=1.577079
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_2_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_2=8,21
PRIMER_RIGHT_2=119,22
PRIMER_LEFT_2_TM=60.577
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=1.7017
PRIMER_LEFT_3_PENALTY=1.577079
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_3_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_3=7,21
PRIMER_RIGHT_3=119,22
PRIMER_LEFT_3_TM=60.577
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=1.7116
PRIMER_LEFT_4_PENALTY=1.577079
PRIMER_RIGHT_4_PENALTY=0.134571
PRIMER_LEFT_4_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_4_SEQUENCE=CAATTCCTAAAGGGTCAAGTGG
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=170,22
PRIMER_LEFT_4_TM=60.577
PRIMER_RIGHT_4_TM=59.865
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_00222 Ps_contig_mira-and-tgicl-ass_franssen_2593
SEQUENCE=GACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAGGAATTGATCCAACAGGTATCCATTACCTGCGTGGATCTCTACTCCATCAAAACCTGCTTTCCATTGCATTTACCTTTTGCGGCAATTTTAAAATCATTGACAAT
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2959
PRIMER_LEFT_PENALTY=1.200250
PRIMER_RIGHT_PENALTY=0.095684
PRIMER_LEFT_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT=9,21
PRIMER_RIGHT=142,22
PRIMER_LEFT_TM=60.200
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=1.3249
PRIMER_LEFT_1_PENALTY=1.200250
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_1_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_1=9,21
PRIMER_RIGHT_1=119,22
PRIMER_LEFT_1_TM=60.200
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=1.3348
PRIMER_LEFT_2_PENALTY=1.200250
PRIMER_RIGHT_2_PENALTY=0.134571
PRIMER_LEFT_2_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_2_SEQUENCE=CAATTCCTAAAGGGTCAAGTGG
PRIMER_LEFT_2=9,21
PRIMER_RIGHT_2=170,22
PRIMER_LEFT_2_TM=60.200
PRIMER_RIGHT_2_TM=59.865
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=1.3363
PRIMER_LEFT_3_PENALTY=1.200250
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_3_SEQUENCE=TCCTAAAGGGTCAAGTGGATGT
PRIMER_LEFT_3=9,21
PRIMER_RIGHT_3=166,22
PRIMER_LEFT_3_TM=60.200
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=1.3770
PRIMER_LEFT_4_PENALTY=1.200250
PRIMER_RIGHT_4_PENALTY=0.176730
PRIMER_LEFT_4_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_4_SEQUENCE=TGTTGGATCAATTCCTAAAGGG
PRIMER_LEFT_4=9,21
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.200
PRIMER_RIGHT_4_TM=60.177
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00223 Ps_contig_mira-and-tgicl-ass_franssen_2598
SEQUENCE=TAGTCGGGGAAGTCGCAGTTACTTCTGCGCAGTCTCTTTTTCGTTCGATTAGGGTTTTCTCTCAACAAGTTCTCTTCCGTTTTCAACCGACAATGAGGGCCAAGTGGARGAAGAAGCGTATGAGGAGGTTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCCAAGTAGTGTGTGTTCTCAGCCATACGTACGTACTAGCTCTTAGTTKTGTTTTTCCCTTTCGTGTTGTTTTGTGGTTATTTAATCAGACTCCTCAGCAAGAGAGTGGTATCTGTCTCTCTCTCTTTGCGCTGTTATGATATTGCGGGTTTGGTATTAGCTCAAAGATATTGATCTTTTTGGAATTGAGTAGCTATAACAGTTGGATTTCGCTCCTATTGTGTGGTTG
TARGET=277,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00224 Ps_contig_mira-and-tgicl-ass_franssen_2607
SEQUENCE=ACTTGGGGAGTGATGGGAAGTATGAGNGTTGGTTAAATGGGTTGATTGAAGAGCCACTTGGGAGGCCTGTGGAGAATGTTGATTCTGAGCTTGTTAAGATGTTTGAAGTGAATAATAATCAAGCCCAGCCCAGTGTTGATAATGGGCTTTCTACGCTCGGTTTGAGTGAGAATGGACCAACTACTGCTAGTGCTTGAGAATTGTTTTATTTCACAAATGAAATACATTACAATTTTTTTCTTCCATTTTTGCATKWTASTTATTWKTCTCTTASTTTTATTTATTTACTTTTACTTGNGTGTATAAGAGTTATTTTAGTTACGAANAAAGTATTAACTAAATTAATTTAACCTTTTCCCGTTGT
TARGET=276,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00225 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1431,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1705
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.143960
PRIMER_LEFT_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT=1311,22
PRIMER_RIGHT=1532,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1705
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.143960
PRIMER_LEFT_1_SEQUENCE=GGTGATGAGTTCTTGTCCATCA
PRIMER_RIGHT_1_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_1=1312,22
PRIMER_RIGHT_1=1532,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2022
PRIMER_LEFT_2_PENALTY=0.058242
PRIMER_RIGHT_2_PENALTY=0.143960
PRIMER_LEFT_2_SEQUENCE=GATTTTCTTGCAATCCCATAGC
PRIMER_RIGHT_2_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_2=1260,22
PRIMER_RIGHT_2=1532,22
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2190
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.143960
PRIMER_LEFT_3_SEQUENCE=CAGAAGATTTTCTTGCAATCCC
PRIMER_RIGHT_3_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1532,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.3056
PRIMER_LEFT_4_PENALTY=0.026532
PRIMER_RIGHT_4_PENALTY=0.279044
PRIMER_LEFT_4_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_4_SEQUENCE=GAGGAGTTTGGGAAACCCTAAT
PRIMER_LEFT_4=1311,22
PRIMER_RIGHT_4=1497,22
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00226 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1447,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1705
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.143960
PRIMER_LEFT_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT=1311,22
PRIMER_RIGHT=1532,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1705
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.143960
PRIMER_LEFT_1_SEQUENCE=GGTGATGAGTTCTTGTCCATCA
PRIMER_RIGHT_1_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_1=1312,22
PRIMER_RIGHT_1=1532,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2022
PRIMER_LEFT_2_PENALTY=0.058242
PRIMER_RIGHT_2_PENALTY=0.143960
PRIMER_LEFT_2_SEQUENCE=GATTTTCTTGCAATCCCATAGC
PRIMER_RIGHT_2_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_2=1260,22
PRIMER_RIGHT_2=1532,22
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2190
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.143960
PRIMER_LEFT_3_SEQUENCE=CAGAAGATTTTCTTGCAATCCC
PRIMER_RIGHT_3_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1532,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.3056
PRIMER_LEFT_4_PENALTY=0.026532
PRIMER_RIGHT_4_PENALTY=0.279044
PRIMER_LEFT_4_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_4_SEQUENCE=GAGGAGTTTGGGAAACCCTAAT
PRIMER_LEFT_4=1311,22
PRIMER_RIGHT_4=1497,22
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00227 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1462,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1705
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.143960
PRIMER_LEFT_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT=1311,22
PRIMER_RIGHT=1532,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1705
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.143960
PRIMER_LEFT_1_SEQUENCE=GGTGATGAGTTCTTGTCCATCA
PRIMER_RIGHT_1_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_1=1312,22
PRIMER_RIGHT_1=1532,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2022
PRIMER_LEFT_2_PENALTY=0.058242
PRIMER_RIGHT_2_PENALTY=0.143960
PRIMER_LEFT_2_SEQUENCE=GATTTTCTTGCAATCCCATAGC
PRIMER_RIGHT_2_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_2=1260,22
PRIMER_RIGHT_2=1532,22
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2190
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.143960
PRIMER_LEFT_3_SEQUENCE=CAGAAGATTTTCTTGCAATCCC
PRIMER_RIGHT_3_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1532,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.3056
PRIMER_LEFT_4_PENALTY=0.026532
PRIMER_RIGHT_4_PENALTY=0.279044
PRIMER_LEFT_4_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_4_SEQUENCE=GAGGAGTTTGGGAAACCCTAAT
PRIMER_LEFT_4=1311,22
PRIMER_RIGHT_4=1497,22
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00228 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1530,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00229 Ps_contig_mira-and-tgicl-ass_franssen_2622
SEQUENCE=GGCGGGGGGGTCTTAGGCCCAAATAATCCACCCTCCCCATACGACGTCGTAGATAAAACTCACATTCCACCGTAATCATCGATCTGAGAACAGAAACTGAAAAAGAGAGAGTGGGAAAACACTTTCAATTTCTTCATCTTCTTCTTCTTGTAATAATAAAACCAGAAAATCGAAACGAAACCGAAATGGGCAAGGATCTGAGCGACGATCAAATTTCTTCCATGAAAGAAGCTTTCACTCTATTCGACACCGACGGCGACGGCAAGATCGCTCCATCGGAGCTCGGAATCCTCATGCGATCTCTCGGCCGGAAACCCNCCAAGCTCAACTCAAT
TARGET=137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7696
PRIMER_LEFT_PENALTY=0.147734
PRIMER_RIGHT_PENALTY=0.621889
PRIMER_LEFT_SEQUENCE=AAATAATCCACCCTCCCCATAC
PRIMER_RIGHT_SEQUENCE=TCGGTGTCGAATAGAGTGAAAG
PRIMER_LEFT=20,22
PRIMER_RIGHT=252,22
PRIMER_LEFT_TM=60.148
PRIMER_RIGHT_TM=59.378
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.9054
PRIMER_LEFT_1_PENALTY=0.147734
PRIMER_RIGHT_1_PENALTY=0.757660
PRIMER_LEFT_1_SEQUENCE=AAATAATCCACCCTCCCCATAC
PRIMER_RIGHT_1_SEQUENCE=GTCGGTGTCGAATAGAGTGAAA
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.148
PRIMER_RIGHT_1_TM=59.242
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.9288
PRIMER_LEFT_2_PENALTY=0.306868
PRIMER_RIGHT_2_PENALTY=0.621889
PRIMER_LEFT_2_SEQUENCE=ATTCCACCGTAATCATCGATCT
PRIMER_RIGHT_2_SEQUENCE=TCGGTGTCGAATAGAGTGAAAG
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=59.693
PRIMER_RIGHT_2_TM=59.378
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=1.0645
PRIMER_LEFT_3_PENALTY=0.306868
PRIMER_RIGHT_3_PENALTY=0.757660
PRIMER_LEFT_3_SEQUENCE=ATTCCACCGTAATCATCGATCT
PRIMER_RIGHT_3_SEQUENCE=GTCGGTGTCGAATAGAGTGAAA
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.693
PRIMER_RIGHT_3_TM=59.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=1.2627
PRIMER_LEFT_4_PENALTY=0.640802
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=AACTCACATTCCACCGTAATCA
PRIMER_RIGHT_4_SEQUENCE=TCGGTGTCGAATAGAGTGAAAG
PRIMER_LEFT_4=56,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=59.359
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_00230 Ps_contig_mira-and-tgicl-ass_franssen_2626
SEQUENCE=CGGGGGTTATTTTGTAAATACAGTTTGGGTTCGTAAAAAAGCGCTTATTCCAAACGCGATCTGTGGTGGTGGTGGTGCTGCAGAAACATCAAGGCAAAACCAAAACCCCCATTCACACACCCGCTAGGGTATCTTTCTTCTGCTAAACCAAACCAAATGGCTGATCCTGAGCTGGAAGCTATCAGACAGAGAAGGATGCGGAGGAGCTTAGTGGGGTCGGCATGGCGAACGGGAAATCCAACCGAACCTGAACAGCAGAGCGCTCGAGGGAG
TARGET=63,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6195
PRIMER_LEFT_PENALTY=2.615161
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=AAAAGCGCTTATTCCAAACG
PRIMER_RIGHT_SEQUENCE=CTGATAGCTTCCAGCTCAGGAT
PRIMER_LEFT=36,20
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.385
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=2.6195
PRIMER_LEFT_1_PENALTY=2.615161
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=AAAAGCGCTTATTCCAAACG
PRIMER_RIGHT_1_SEQUENCE=ATAGCTTCCAGCTCAGGATCAG
PRIMER_LEFT_1=36,20
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.385
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=2.6806
PRIMER_LEFT_2_PENALTY=2.676221
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=GCTTATTCCAAACGCGATCT
PRIMER_RIGHT_2_SEQUENCE=ATAGCTTCCAGCTCAGGATCAG
PRIMER_LEFT_2=42,20
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=59.324
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=2.6806
PRIMER_LEFT_3_PENALTY=2.676221
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=GCTTATTCCAAACGCGATCT
PRIMER_RIGHT_3_SEQUENCE=CTGATAGCTTCCAGCTCAGGAT
PRIMER_LEFT_3=42,20
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=59.324
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=2.7233
PRIMER_LEFT_4_PENALTY=2.615161
PRIMER_RIGHT_4_PENALTY=0.108177
PRIMER_LEFT_4_SEQUENCE=AAAAGCGCTTATTCCAAACG
PRIMER_RIGHT_4_SEQUENCE=AGAAGAAAGATACCCTAGCGGG
PRIMER_LEFT_4=36,20
PRIMER_RIGHT_4=140,22
PRIMER_LEFT_4_TM=59.385
PRIMER_RIGHT_4_TM=60.108
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00231 Ps_contig_mira-and-tgicl-ass_franssen_2627
SEQUENCE=TTCTTACTTAATTGTTCTTGGAGATTCTGATGATGTACTTGAGCATTTGGAGTGATTCTTGTATTTTAATTGTGATATGTGATACAAAACTTTGATACTTTCCCCCTTGAGCCATGTATTATGTACACTACCAAGGCTTTCTTTTGGTCTTGTGTTAATATATTGTAGACATTTATGAAGTCTAAGTTTTWRWAAMSTCTATTCATTGTCTATGTTATTTTTATTTACAACAGACCAGAGATATACAACGTGGAATAAGTGTAATATATAAATAAATAAAACTAAAAATT
TARGET=268,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00232 Ps_contig_mira-and-tgicl-ass_franssen_2637
SEQUENCE=GTAGTGAAAACAAAAACTCAAGCTTTTGAGGTCACTTATTTTCGTTCCTAGTAATTCGGATATGGTGGTTCTCAGAGTTTTGGGTCTTTGGAATGGAGTGATGGAGTTCATATTGTGAGGAGCCCAATTGCTGCTAGATGGAGCAATGGATTCTCATCAGCATTCCYTTTTGTAWTTTTTCATTCTGTAGAAAGTGGAAACACATTATTATTATTACCATGCATAGGTAGGAAACATTGTTATGTATGTAAAGTTAATTAGTTCTTGTTAATTAGTTAAGTTAAACTTTTAATTAGTTAGTTACTTTTAATTAGTAAACAAAACTAAAAACAAAGTAAAA
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00233 Ps_contig_mira-and-tgicl-ass_franssen_2638
SEQUENCE=AGTTAAACAAGAAACAAACCAAACCAAATAATTATTCTTGTTTAGTTTGTTACAATAAAAGACTAACATAAAAGTTAACAACAATACAAGACTAATTAACTTTCATACATAACATGTTTCCACCTATGCATGGTAATAATAATAATGTGTTTCCACTTTCACAGAATGAAAAATCAAAAGGATGCTGATGAGAATCCATTGCCTCCATCTAGCAGCAAGTTGGGGCCTCCCTACAACAATATKMR
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00234 Ps_contig_mira-and-tgicl-ass_franssen_2639
SEQUENCE=TTTGGGTTCTTTGGAATGGAGTGATGGAGTTCATATTGTGAGGAGCCCAATTGCTGCTAGATGGAGCAATGGATTCTCATCAGCATYCYTTTGTATTTTTCATTCTGTGAAGTGGAAACACATTATTATTATTACCATGCAAKTAGGGTAGGAAAMMTWGTTATGTATNAAGTTTAATTAGTTCTTTAGTTATTTAGTTTAGTTTAAAC
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00235 Ps_contig_mira-and-tgicl-ass_franssen_2686
SEQUENCE=GCATAAGTTTCTAGCGACGTATCCATCCAATTACTAAACTTGGTCGACCTCCTCAATCTTGGGACCAGGGCCGCCACTGGCACTTGTAGGTGGAGGAACTTCATCATCATCACCACCCATGTCAGGAGCAGCTCCACCTTGGTACATCTTAGCAATTATTGGGTTGCAAAGACTCTCAAGTTCCCTCATCTTATCTTCAAACTCATCTGCTTCAGCCAACTGGTTATTATCCAACCAAGTAATTGAAGCATCAATTGCATCTTCAATTCTTTTTCTTTGTCATCAGCCGAAAGCTTTGAAGCAATTCTTTTCATCCTTAATCGTGTTCCTCATGTTGTAAGCATTAGTTTTCCAGTGCTATTTCTTTGCACTACCTAACCTTTTCCTTTCTTTAGGTTTCGTTTCCCCTC
TARGET=101,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2122
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.205226
PRIMER_LEFT_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_SEQUENCE=AACTTGAGAGTCTTTGCAACCC
PRIMER_LEFT=6,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.795
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.2276
PRIMER_LEFT_1_PENALTY=0.006951
PRIMER_RIGHT_1_PENALTY=0.220659
PRIMER_LEFT_1_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_1_SEQUENCE=TAATAACCAGTTGGCTGAAGCA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.2604
PRIMER_LEFT_2_PENALTY=0.006951
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_2_SEQUENCE=TGAGAGTCTTTGCAACCCAATA
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=0.3929
PRIMER_LEFT_3_PENALTY=0.006951
PRIMER_RIGHT_3_PENALTY=0.385922
PRIMER_LEFT_3_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_3_SEQUENCE=GGTTGGATAATAACCAGTTGGC
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=59.614
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.4365
PRIMER_LEFT_4_PENALTY=0.231280
PRIMER_RIGHT_4_PENALTY=0.205226
PRIMER_LEFT_4_SEQUENCE=AGCGACGTATCCATCCAATTAC
PRIMER_RIGHT_4_SEQUENCE=AACTTGAGAGTCTTTGCAACCC
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.231
PRIMER_RIGHT_4_TM=59.795
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00236 Ps_contig_mira-and-tgicl-ass_franssen_2691
SEQUENCE=ACTTTTTTTAACGTTTTAGTCAATNTTACATTACTTTATTGTAAATCAAAATTAACTTGGAACTTTAATTTTTTTATTTTCTAATTACAAATAAAATTAATAATCTTTAAAGAATCCAAAGTAAATAGCAAGAAATAACACAGGTCTGGGAAGGAGGAGAAAAATTGAAGAAATGTATCTCACATACATACATGTAACAGCTTTATTTCTATATCTCGAACAAAAATAGGGTATGATTTCCGGCTAGTCTCTCATCGAACTTGGCGCGATAAGATTTGACATAGTTATTGGCAATAATTATCTTCTTTCCAAAAATGAAAAATGTAATTTGAAAGCAGCTAGTTGACAACATCAAGAACCAACTTGCGAGACTTCAGAATGGACCATCGAAGCCGACGATAATATGCGGCTTGAAGTAATGCCAATGATAGTACAAATATCCATTTAAATCCCATCAGTTTTCTCTCTTCCATCTCCGGTTCTGCAGCCCAAGACAAAAATGACACAACATCTTTCCCCATCTGAGATTCTGTAGCAGGGGTACCATCTTCATATTCAACAGCACCATCATTAAGCATTTTAGGCATGGCAATGGCTCCGCCTMGG
TARGET=182,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00237 Ps_contig_mira-and-tgicl-ass_franssen_2702
SEQUENCE=TTTTATGCATATATATATATATAAAAGACAAGACTATGTTCTATTTAATGTCTTAAGACTGTTTTTCAGGTGTACGTATGTTTATTGTTACGAGCATATATATATGATTATTCTTAAGAATAACT
TARGET=9,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00238 Ps_contig_mira-and-tgicl-ass_franssen_2702
SEQUENCE=TTTTATGCATATATATATATATAAAAGACAAGACTATGTTCTATTTAATGTCTTAAGACTGTTTTTCAGGTGTACGTATGTTTATTGTTACGAGCATATATATATGATTATTCTTAAGAATAACT
TARGET=96,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00239 Ps_contig_mira-and-tgicl-ass_franssen_2741
SEQUENCE=GGAAAAGAAAAAGAAAAGTAAAAAGWSGGAGTACTAGTGACCGGTGAAGCAGAGGGAAAATTGAAGAGAAAGAAAACAGTTGATTCCGACGAAAAAGAGTGAAATCCAAGCTTCAAATTTACGATGGATGTGATCAAAACGCAGCAAATCTCATCTCGATCGATCGAGAAGGTGGTGGTTCATCCATTAGTTTTGCTCAGCATCGTCGACAACTACAACAGAGTCGCCAAGGACACACGGAAGCGCGTCGTCGGCGTCTTGCTAGGTTCCACTTTCAAAGGCACCGTCGACGTTTCCAACAGCTACGCAGTGCCCTTTGAAGAAGATGACAAGGATCCTAGCATTTGGTTTCTCGACCACAATTACCATGAAGCAATGTTTTCCATGTTTAAGAGAATAAACGCAAAGGAGCACGTCGTGGGGTGGTATAGCACAGGTCCAAAATTGCGTGAAAATGACCTCGACATTCATGGGTTATTTAATGACTATGTTCCAAATCCAGTGTTGGTTATAATTGATGTTGAACCTAAGGAGTTGGGAATTCCAACAAAAGCATACTATGCTGTTGAGGAAGTTAAAGAGAATGCTACTCAGAAAAGCCAAAAGGTCTTTGTGCATGTGCAATCCGAGATAGCTGCTCATGAGGTTGAGGAAATAGGAGTGGAACACTTGCTTAGGGATGTAAAGGATACAACCATCAGCACCCTTGCAACCGAGGTGAGTGGGAAACTCACAGCCTTGAAAGGTTTGGATGCAAGGCTTAAAGAGATAAGGAGTTACCTTGATCTTGTAATTGATGAAAAGCTTCCCTTAAACCATGAAATCCTATACCATCTTCAGGATGTGTTCAACCTGCTACCAAATCTTAATGTGTCTGATCTTATCAAGGCTTTTGCAGTGAAAACCAATGATATGATGCTGGTAATATACCTATCATCTCTCATTAGAAGTGTAATTGCACTCCACAACTTGATTAACAACAAGATGCTCAACAAAGAACATGAAAGGGCAGAAGACTCAAAATCTGTACCGGTGCCAAGTGCATCTGCGTAAGGTTTGAGGGTTGTAATGGTTTGAGCTCTAAAGGCACCGTGATTGATTCCATGGACAGATTCTTACAACTTAAATTGCTTTTGTCAGCCCTCAAGCATTTCTGATAGGGTAGAGAAATACATGTAACCTAGCATAACGATCCTTTTGCATCATTTATGTATTTCACTCTTCTATCCTATGATCATATCCTTAGTTTAGTATCTTTGTTAACATCTGTTGAATAACTTGTTAAAAACGAATTGATCACCGATATCAGGAATCAAATGTCTCCTTAATTGTTTTTTATAATATTTATCACTTGCCCAAT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00240 Ps_contig_mira-and-tgicl-ass_franssen_2747
SEQUENCE=CTAAACAAATAAAAAATAAATAGTACCAGACAAGAAATAACCAAAAACGTACCGGGACAACTACCAGTTCTAAGAATAAGAAAAAAAAGTAACAAAAAGGTGGACACAATCCATGAAATTKTTAAGATATTAAACTCTCTTCTCAATCTAATTCAATCAAGCATGTCTGTTCCTCTTCTTGGCACCAGACTTTGCAACTTTGAGGCTTCTGTTAACAACACTCAACCTTGCAAGAGCAGCCTTCTTGAGATCTGGTCTGTAGTAGTTATCTGCCACCTGGTTCTGAACAGCCTTAGCCATTCTCCTAAACTCTTTCTTCATCACAGATTTGTGAAGCAAAGCCGAAGGCTTGTTCTGCTTCCTTGGCTTGGAGGTGGCCAACAACACAGACTGATCCTTCCCAGCTGACTGAATAGTAACAGTTTTCTTGTTGGCCAAACCAGAGTACTTGTAGGAGTTGAGGTTGTAGAGATTGTTTGGCTCTCTGCTAAACTGAACACTCTGAGTGTTGTTACCAAACTCCTTGACCAAGAAAGAGTTGTTCCTCTTCACAATCTCCCAAACAAGAGGTCCAGGTACTGTTGTCATCTTCTTCTTCTACGAGCAAACCCTTTGGCTGTTGTGAGAGAAATGCGATTTGAGAAGAAAGAGTAAGCCGCAAAGCAATATATGTAGAGAGATCCCCGACGTACTCTGCGTTG
TARGET=588,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00241 Ps_contig_mira-and-tgicl-ass_franssen_2748
SEQUENCE=TCTGGTCTGTAGTAGTTATCTGCCACCTTTGGTTTTCTTTGKTAACTWAGTCCTTAGTCCATTCTCCTAAACTCTTTCTTCATCACAGATTTGTGAAGCAAAGCCGAAGGCTTGTTCTGCTTCCTTGGCTTGGAGGTGGCCAACAACACAGACTGATCCTTCCCAGCTGACTGAATAGTAACAGTTTTCTTGTTGGCCAAACCAGAGTACTTGTAGGAGTTGAGGTTGTAGAGATTGTTTGGCTCTCTGCTAAACTGAACACTCTGAGTTGTTGTTACCAAACTCCTTGACCAAGAAAGAGTTGTTCCTCTTCACAACTMMWCCWAANAAGGACGGTCCGTAACGGTTACTTGGTTTCGTACTACTTTCCTTTCCTTTCCTTACCTAACGACGAACCCTTAAACCCGTTTGGTCGTTGGTTTGGTAGGAAGGAAAGTAAAACGTCGGTACCGTTTACCCCG
TARGET=365,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00242 Ps_contig_mira-and-tgicl-ass_franssen_2764
SEQUENCE=ACATGGTATCAACGSRGRTATCATCATTATGCAGCCTTATCGTTAACACTATTTCTCTCCGTTTTCTGAAATTACCTTTTCACAACAATGAATTCATCATCGCTACTTCGTCCTTCTCCTCCTCCTCTTCTTTTTCATCGCAACCGTTTCGTTATTTCTTCTTCATCTAGAAACCAATCTTACATCCCTAAACTCGAACCTTTTAGCCGATCCAAAATCGACAGAGTCGCTAAAAGCCTCCCTATCATTAGAAAAATCTAGAAAAAGATCTCTCCGATTATTGTTCTACACTTGAAGGTGATGAATGTTATAGTTGCTGGCAGGCTTATTTTGAGCTACAAAGATACTTACAAAAGGNTCACCGAGAGGAGATATAGAGAGGTTAATTATTCAGATTGGTGGTAGTAAATCCTTGATAGGATTAGTTTACAGTTAGGTAATTGAC
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00243 Ps_contig_mira-and-tgicl-ass_franssen_2793
SEQUENCE=TAAGATTTYTTTTYTTAACTWAATACAATAGATCATATTATAACAATCTTGTTTGTAAACAAAAAGTAAATACGCTAAATTCAAAATACAATCTTGTACCAGTACATAAAAGAACAAAAATAAAACAAACTGCATAACAGGTAAAACAAAATAACTACAACAAAAAACAAACTAGGAAAAATGCAACCCACGTCAGAGTTTGCCGGGAATGAAGTTTTGTTAAAACACAATTATCTTCAGTAAGCTCTTAATGTGCTAACTGATAAAACAAGCAACTCCAATATGAGAAAGGAAATGTGATTACTGAAGAAAGAGGAAAACGAGGAAGTAACTGCTGCTGCTGAACTCCTTGTGAAATTGAGTGTTGA
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00244 Ps_contig_mira-and-tgicl-ass_franssen_2817
SEQUENCE=GGGGTATCAAGATCCAACGGTCCATAATGTACGAGCGTTCTTACTCCTATTTTTTCAGAGGTTTTAGTGCTTCAAACCCCAGATCTTGCTTATCAGTCTCTAGATCTTCGTTCCTGGAATTAAAAATTTTCTGGTTTGAAGAAAAGCTTGTTTGTTTGTCACACGAATGGAGACTGAACACTAGTTCATNATGCACGCGCGGCGACTTACTGC
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2774
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=1.276629
PRIMER_LEFT_SEQUENCE=TTTAGTGCTTCAAACCCCAGAT
PRIMER_RIGHT_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT=62,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=58.723
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=1.3107
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=1.276629
PRIMER_LEFT_1_SEQUENCE=CAAACCCCAGATCTTGCTTATC
PRIMER_RIGHT_1_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=58.723
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=1.3107
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=1.276629
PRIMER_LEFT_2_SEQUENCE=AAACCCCAGATCTTGCTTATCA
PRIMER_RIGHT_2_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=58.723
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=1.3117
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=1.276629
PRIMER_LEFT_3_SEQUENCE=GTTTTAGTGCTTCAAACCCCAG
PRIMER_RIGHT_3_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=181,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=58.723
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=1.3581
PRIMER_LEFT_4_PENALTY=0.081468
PRIMER_RIGHT_4_PENALTY=1.276629
PRIMER_LEFT_4_SEQUENCE=GTATCAAGATCCAACGGTCCAT
PRIMER_RIGHT_4_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=58.723
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_00245 Ps_contig_mira-and-tgicl-ass_franssen_2829
SEQUENCE=TGAAATATTCCCCGTCCCAAATTGATCATCCTTCCCAACAGCAGGAACAGGCGGCCCTTCCATCCCCGAACCCGTTGTCCATCCTCTACCAGTTGCAGCACTCAACACCAGCCCCATCGCAGCATCATTGGAAAGAGGGGAAATGGAGAAACAGCGGTTACTGGGAACTGGAAGGTTGATGTTGAGGTTGATCTCGTCGGAATCGTTGGCATCGTCACGTTTGGAGGAGGGTAGACGAAAGTGGTAGGTTTTCTGGAAAGGAAARGGKGATSKTRTTCGTTTGGTTTGGTTTGGTTTGGTTTAGGAAAGGGGAAGGAACGGGAAGGAAAAGGAAAGGGTAGTCGGTTTGTTAGGAAAGAAGATGGTAAAGGTTAAAG
TARGET=279,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00246 Ps_contig_mira-and-tgicl-ass_franssen_2834
SEQUENCE=TGGATCTAGGTTTTCTAAAATTTTGTTAGGTTTTCTTCAATTTTAAGTTAAGGATTCACCGATTTTGTGGTTAGATTTTATTGAATTGAATTGATTGAAAATTTGGACTTATTTTGTGTGATTTGTGATTAGGTTTTGGAATTGGCTGGAAATGCTGCAAGGGACAACAAGAAGACTAGAATTGTGCCAAGACATATTCAATTGGCTGTTAGGAATGATGAAGAATTGAGCAAGCTTCTCGGTGATGTTACCATTGCTAACGGTGGTGTTATGCCCAATATTCACAACCTGTTNCTCCAAGAGGCTGGTACCTCCAAGGGTNCTGCTGCTGATGAANATNATNATTCTGACGACACGCAAACGGG
TARGET=80,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9569
PRIMER_LEFT_PENALTY=0.939039
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=GGATTCACCGATTTTGTGGTTA
PRIMER_RIGHT_SEQUENCE=TAGCAATGGTAACATCACCGAG
PRIMER_LEFT=51,22
PRIMER_RIGHT=258,22
PRIMER_LEFT_TM=60.939
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.9832
PRIMER_LEFT_1_PENALTY=0.939039
PRIMER_RIGHT_1_PENALTY=0.044122
PRIMER_LEFT_1_SEQUENCE=GGATTCACCGATTTTGTGGTTA
PRIMER_RIGHT_1_SEQUENCE=CTCTTGGAGNAACAGGTTGTGA
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=60.939
PRIMER_RIGHT_1_TM=60.044
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=1.0732
PRIMER_LEFT_2_PENALTY=1.055315
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=TAAGGATTCACCGATTTTGTGG
PRIMER_RIGHT_2_SEQUENCE=TAGCAATGGTAACATCACCGAG
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=61.055
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=1.0994
PRIMER_LEFT_3_PENALTY=1.055315
PRIMER_RIGHT_3_PENALTY=0.044122
PRIMER_LEFT_3_SEQUENCE=TAAGGATTCACCGATTTTGTGG
PRIMER_RIGHT_3_SEQUENCE=CTCTTGGAGNAACAGGTTGTGA
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=61.055
PRIMER_RIGHT_3_TM=60.044
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=1.1722
PRIMER_LEFT_4_PENALTY=0.939039
PRIMER_RIGHT_4_PENALTY=0.233127
PRIMER_LEFT_4_SEQUENCE=GGATTCACCGATTTTGTGGTTA
PRIMER_RIGHT_4_SEQUENCE=NAACAGGTTGTGAATATTGGGC
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=293,22
PRIMER_LEFT_4_TM=60.939
PRIMER_RIGHT_4_TM=60.233
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_00247 Ps_contig_mira-and-tgicl-ass_franssen_2859
SEQUENCE=GGCCTTTTCAGAATCTTATTCCCAAAATTCGTCTATTCGTACAATTTCGCAGGCTTAACGCCCAAACCCTATTCCCCCAAATTCTCTGTCTTTCTCTTCACTTCACTTCATCTACAACAATGGGATCCTCCGCGATGGTTTTAGACCCAAAACCCGTCTCAGAACCGCCGCCTTCTCTTCTATCATCAAAATCCGATTACCTTTACGGTGGCGACTCAGCCTCCGATGAAGATGACCTCTACAGCCGCCTCAAATCTCTGCAGCGACAACTAGAGTTCATCGACATCCAAGAGGAGTATGTAAAAGATGAACAGAAGAATCTAAAGCGCGAGCTTCTTAGGGCTCAGGAAGAAGTTAAGAGGATTCAATCTGTTCCACTCGTTATCGGTCAGTTCATGGAGATGGTTGATCAGAACAACGGTATTGTTGGATCAACTACTGGATCCAACTACTATGTCAGGATTCTCAGTACTATCAACCGTGAGCTTCTCAAGCCTTCTGCTTCGGTTGCTCTTCACCGACATTCCAATGCACTTGTTGATGTTCTTCCGCCGGAGGCTGATTCTAGTATCTCGCTACTTAGCCAGTCTGAGAAGCCTGATGTTTCTTACAATGATATTGGAGGATGTGATATCCAAAAGCAGGAAATTCGCGAGGCTGTGGAGTTACCTCTCACACATCACGATTTATACAAGCAAATTGGTATTGATCCTCCTCGAGGTGTTTTACTATATGGCCCCCCTGGAACCGGTAAAACAATGCTTGCCAAAGCTGTTGCTAATCATACCACTGCCGCCTTTATCAGGGTTGTGGGCTCTGAGTTTGTGCAGAAGTATCTTGGCGAGGGTCCAAGAATGGTTCGTGATGTATTTCGTCTCGCAAAAGAAAATGCCCCTGCAATTATATTTATTGATGAGGTTGATGCAATAGCTACTGCTAGGTTTGATGCCCAAACTGGAGCTGATAGAGAAGTACAACGTATCCTCATGGAACTTTTGAATCAGATGGATGGTTTTGACCAGACAGTGAATGTCAAAGTCATAATGGCAACCAATCGAGCAGACACTTTGGATCCCGCCCTCTTGCGTCCTGGAAGACTTGATCGGAAAATCGAGTTCCCCTTGCCCGATAGACGCCAAAAGAGGCTTGTATTTCAGGTTTGCACAGCAAAAATGAATTTGAGTGATGAGGTGGACCTGGAGGATTATGTTTCCCGTCCTGACAAAATTAGTGCTGCCGAGATATCTGCTATTTGTCAAGAAGCGGGGATGCATGCTGTTCGTAAGAATAGATATGTCATACTGCCAAAGGACTTCGAGAAGGGTTATAGGACCAACGTGAAGAAACCTGATACCGACTTTGAATTTTACAAGTGATGATGGAGGGTATTTTTCTGTATTTTGTTTGTGTATGTATGTGCTACGTTTCAGAATATAGTTGAAGGTTCAGAATAKAGTTAAGGTNAG
TARGET=96,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00248 Ps_contig_mira-and-tgicl-ass_franssen_2874
SEQUENCE=CAAAATATTCATTCCAAAAGTGTTGTCAAATTTTTTAGTAATTCCACCCTCTTTAWTTGAATTTGTTAAGTGGTACCACACGTCTTTGAGAACTTGGTCGTGTTGCATGAGAAAGAAAGAAATCATGGAGGCACCATCTTCATCTTTATTATTATTTTCGACAAAGTATTTAGCTAAAGGGTGAACACCATACAATCTTTCTTCATTACAATYTCCATCAACTTGTTCAATGGAACATGTCAGAATTTTGTAGCTAGMCAAAAGACGCAACAATCTATCCAACATAGAAGCTGCGTCTGGATTT
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00249 Ps_contig_mira-and-tgicl-ass_franssen_2895
SEQUENCE=TTACATCTGATTAGGTGAATCATTCTCTTTCTCTTTCTCTTTCTCTTCTTCRWACATTCAACACCCAAACACCTCTCTCTCTCTCTCTCCGCATATAACTCCTCCTGCTATCATCCACATAGACACAACAACCACCTGTGAAGATGGGTAGGAGCTTAAGTTAAGGCTGCACGTCGGGAGACGCAGTTATTGTACCTTTCAGTTGGGTTTGGGTTTTTTTCTTCCTTCCAGTTGGG
TARGET=73,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00250 Ps_contig_mira-and-tgicl-ass_franssen_2896
SEQUENCE=CGGGTTTTTAACTTTTTTTACTTTTTTAAACTTTTTCTTAACTAAWTGAACAACAACATCAACAACAACACAAATCCATTTTGAATTCTCTCTCTCTCTCTCTATTGAATGGAGTCTTTCTCTTACACTAACACCAACCCTTCTCATCTTCNCTTGTCACTTCTCTAACAAGCTATGTGTAGTTTCATCAAAGGAAAGGTAAAGGTTTCTTTTC
TARGET=87,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00251 Ps_contig_mira-and-tgicl-ass_franssen_2899
SEQUENCE=TCGGGGGAACACAAACTCAGSATGAACAACAACATCAACAACAACACAAATCCATTTTGAATTCTCTCTCTCTCTCTCTATTGAATGGAGTCTTTCTCTACACTAACACCAAACCTTCTTCATCTTCTACTTGTCACTTCTCTAACAAGCTATGTGAGTTTCATACAAAGGAAAGTAAAGGTTTCTTTTCTTGAT
TARGET=63,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00252 Ps_contig_mira-and-tgicl-ass_franssen_2904
SEQUENCE=GCTCTGAAGTATTTTAGGGTTTTTGTTTCCGTGAACAGTTTCTTCTTAGGTCTGAGCCAATATCCTCTGACAAAAATGGTTCTTACAAAAACGATTATTGTATTTTACGTTAAACCCCTCCTGCTGAGCTTAGAAGAAGAAGAAGCAACAAGCTCAAGTCGTTCTTGTTCACGTACCAACCAACTTCTTCT
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2278
PRIMER_LEFT_PENALTY=0.163658
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=TCTGAGCCAATATCCTCTGACA
PRIMER_RIGHT_SEQUENCE=GTTGGTTGGTACGTGAACAAGA
PRIMER_LEFT=50,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=59.836
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.2376
PRIMER_LEFT_1_PENALTY=0.163658
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=TCTGAGCCAATATCCTCTGACA
PRIMER_RIGHT_1_SEQUENCE=TTGGTACGTGAACAAGAACGAC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=178,22
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.3292
PRIMER_LEFT_2_PENALTY=0.265053
PRIMER_RIGHT_2_PENALTY=0.064105
PRIMER_LEFT_2_SEQUENCE=AGGTCTGAGCCAATATCCTCTG
PRIMER_RIGHT_2_SEQUENCE=GTTGGTTGGTACGTGAACAAGA
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=183,22
PRIMER_LEFT_2_TM=59.735
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.3390
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=0.073989
PRIMER_LEFT_3_SEQUENCE=AGGTCTGAGCCAATATCCTCTG
PRIMER_RIGHT_3_SEQUENCE=TTGGTACGTGAACAAGAACGAC
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.3565
PRIMER_LEFT_4_PENALTY=0.292414
PRIMER_RIGHT_4_PENALTY=0.064105
PRIMER_LEFT_4_SEQUENCE=TGAGCCAATATCCTCTGACAAA
PRIMER_RIGHT_4_SEQUENCE=GTTGGTTGGTACGTGAACAAGA
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=183,22
PRIMER_LEFT_4_TM=59.708
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_00253 Ps_contig_mira-and-tgicl-ass_franssen_2924
SEQUENCE=CRTTCCAAATACTCAATAAATTAAAATCACGTAAATAACTTAAACAAACCTATTTGATGTCTCTGATGTCTCTGCAACTTAATAAAACCAACATGTAACTGAGCACAAGGTCCAGACAAACAAACAAAATTTGGTAACAAGAAAATTAAACAAAACATAAATCATAGCAACAATGTCTAAACGCAAGAAATTAAAAAATTGTTCACTGATAATATCTCTTCTGACAAGACAAAAAACAGAAACTACTTTATCCGTCTTSGWTASRSAACTGTTTTTCC
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00254 Ps_contig_mira-and-tgicl-ass_franssen_2935
SEQUENCE=GAAGCCAAGTAAGGCKTCKGRGGACCTAAGAGTGCAAAGCAAACAAGAGCAGACTTAGAAGCTACTTCGAATTAGGTAAAATATAATTTGACATGGGAAATAGTCTCAACGGTGTTAGTCTCTTCAACGACGACACAGATTATCCATGTCGGATACAGCTAGGTAGATATGCATTCATCGATGTGACACAAACAGAGAACAAAGGCGATTGCAATGATAGTGATGTGAGGCAAACAAAGAACAAAGGCGATTGCAGTGATAATGATGATGAAATTTCCAGTGATAATGATGAAGCTCCCAGTGATAGTGATGATGAAGTTCTCAGTGATGATGATGAAGTCCCCGGTGATAATGATGATGAAATTCTCAGTGATAGTGATGATGAAGTTACCAAAGTGACCAAGATGTTATACACCGTAGGTAGGTCGTCCATATGTGAACTCAAATTTAAGAGAAGACATAAAGAGGCATCATTATCTCTCCTTGATGTCGAGAATGGTTCCAATTCCAATTGTGTGGATGTTAAAATTATAATAAGAAAAACTGATAAGGGATATCTCGAGATACCTTATCCTGATTTCTCCTTTAGAGGCGGAGGGATATACATTTTCATGAGGGATAATAATTCTAACAGAACCATCAATGCATGGGGAGGTGTGACAGAATTAACAACCTTTCTCTATGGTGAAGGAAGGAGGGTAAGTCTTGTTGTTATAGAGTCTAAGAAAAAGAGTATAAGTGATCCAAATCCCTATATGGTGACTGTGGTTCACTATTTTGCTATTGATGATGGAAATTTTTTCAATGCAAAAATGGATATTGGCCTTTCTGTGATGGTAAAGATTCAACCATCAGATGATTTGGGATTGAAATATACTGTAGAGGGTCCAGAGAGACACCCTAGTTCATCATTATTTTACATGTTTGAACGAGTAAAAAGACTTAATGGGGCATGGAAGTCTAATTTGTGTCCTCATTGTGCAAATCTTCAGATGCGGCGTAAATCCTGGCAGACCTCTGAAAGTGAAGACAGCGATTATGTTCCACGACGACATGGTAATGGTAAGCAGAAGTCAGAAAGTATGGTTGCCAATGATTCTAACGTTAAGGGAAATTATAATGGTAGTTTGTTTGGGAAGTATTTCCATTTTCATACAAGGTGAAATATGTAATGGAATCCTTTAATATGAAAAGAAAGAAAATAAAAAGGTTAGGGCAAGGTTAGCCACGGCTAAAGATGGATACCATACCTAACTAAGTATGTTGTTAATTAGAGAGCATTTTAAAATATTGGTAAGTAATTTTGTGTGTTGCACACACCTAGTTGAATGTAAAGAGAGAGGGTTAGCTATCTTCCATGAATGTAATATTTGACACTTAAGATTTGATCATACCTTTAATGTCAAATTACTTGAAATAAATAAAAAAAATACTTTTGTTCAACC
TARGET=326,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00255 Ps_contig_mira-and-tgicl-ass_franssen_2959
SEQUENCE=GTCTAGAAAGTAGGCGTTCACTGGAGTTCAACTACTTTTGTTGTTCCACCTTTCCTTGCTCTCAACCTTGAGTGCCTGCAGAGCTTTCCTTTTCTCTTCTAAAACTTTCTCATATTCTTCTAGTGTCATTTCCTTGTCCTCAGGCTCCTTTTCTTCAGCTTCATTAGCAGGTGCTTCTTTGTTTCCTTCAGCAGCTGCATCTTCTTCACCAGCAGGCTTCTCCTCAGACACATTCTTTTCGGTTTCGTTAGCAACTTCATCAGTCACCTGAGCAATTTCATCTGACTGAGTTCCCCAATTACCACGTCCAGCACCTTCACGTTTGAATCCGCCTCCTCGTCCAGTCCCGCTGTGGCGATCAAATGTCCTTCGTGGACGTCCTTCTTCACCACCTTCACCATTGCTAAATCCTCCACGACGACCGCCACCTCCGCCGACACGGTAAGGAGCACGAGGTGCACCATAACCACGCCTTTCAGAGGGATTCCCAGAATCTCCTTCAAAAGAACCTTGATTAGCAGGAGGAACAGGCAGTGAAGAGGAGTTATCGTCATTGGAAAAGTCACGACCAAAGCCGCCGCCGCCGCCACCACCACCGCGACCACGTCCAAATCCTCCTCCATATCCCCGTCCGCCTCCACGGCCACCACGAGATGGTTCGTTTCTTGACTCCCTCACAGCCTGAGAGGGAGGTAGTGGTTTGGAAGGCAAAAGAGCAGCCTTTTTGGGAGCAACTTGTTTGCCTTGTTCCAAACCCTTTTTGGGAGCAGCCG
TARGET=574,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0386
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=TATCGTCATTGGAAAAGTCACG
PRIMER_RIGHT_SEQUENCE=CTTTTGCCTTCCAAACCACTAC
PRIMER_LEFT=545,22
PRIMER_RIGHT=713,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.1124
PRIMER_LEFT_1_PENALTY=0.077340
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GAACAGGCAGTGAAGAGGAGTT
PRIMER_RIGHT_1_SEQUENCE=CTTTTGCCTTCCAAACCACTAC
PRIMER_LEFT_1=524,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.1280
PRIMER_LEFT_2_PENALTY=0.093026
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=AGGCAGTGAAGAGGAGTTATCG
PRIMER_RIGHT_2_SEQUENCE=CTTTTGCCTTCCAAACCACTAC
PRIMER_LEFT_2=528,22
PRIMER_RIGHT_2=713,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.1567
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.153161
PRIMER_LEFT_3_SEQUENCE=TATCGTCATTGGAAAAGTCACG
PRIMER_RIGHT_3_SEQUENCE=GTGAGGGAGTCAAGAAACGAAC
PRIMER_LEFT_3=545,22
PRIMER_RIGHT_3=677,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=60.153
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.1633
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.159665
PRIMER_LEFT_4_SEQUENCE=TATCGTCATTGGAAAAGTCACG
PRIMER_RIGHT_4_SEQUENCE=CAAACCACTACCTCCCTCTCAG
PRIMER_LEFT_4=545,22
PRIMER_RIGHT_4=702,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.160
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_00256 Ps_contig_mira-and-tgicl-ass_franssen_2981
SEQUENCE=GGATTTTTGTTTGTTTGTTTCTGAATCGGAACTTTACTTTACTTGTATTTTACATAAAGCATGTTCTGTCCAAGATCACTGCTCCTCCCCAAAAGCTCCTTTACCCTCTCTTTCTTCTCCCATCGCTTCACCTGTAACTACAAGATTGTTGAAATGGCGCGATCTTCGTTGGATGAGATATCAGACTCGGGAGCATTCACAAGATCTGCTTCCACATTCCGACAGTTCGTTTCAAGGGATCTTAATTCCCTAATTCCACCTGAGTCTGGAAGTNTCATCTNTACATNTNAT
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00257 Ps_contig_mira-and-tgicl-ass_franssen_2986
SEQUENCE=CTTTAGCAATTGAGACTRCTTATGAGGRCCTTGAACGATCCGGCTTCTCTGATGGTAAATCTATACTTGTTCTTGGCGGTGCCGGTGGAGTTGGAACCCATATCATTCAGCTTGCCAAACATGTTTTTGGTGCTTCCAGAGTCGCAGCTACATCTAGCACCAAGAAACTAGAATTGTTAAGAGAGTTAGGAGCTGATTTGCCTATTGATTATACAAAGGAGAATTTTGAAGAGTTGGCTGAGAAGTTTGATGTGGTGTTTGATACAGTCGGGGAAACTGAGAAGGCATTCAAGGCTGTGAAAGAAGGTGGCAAAGTTGTGACAATTGTGCCGCCAGGAATTCCACCAGCAATAGTGTTCATACTTACTTCAAATGGGGCTATCTTAGAGAAACTAAAGCCTTATTTGGAGAGTGGAAAGGTGAAGGCAGTGCTTGATCCCAAAAGTCCATTTCCATTTTCTCAAGCTGTTGAAGCATTTTCCTATCTCGAAACTGGCAGAGTTACTGGAAAACTAGTTATCCATCCCATACCATGAAATTCAAAACAACCCAATACAATCTTTGGAATCTGAATGTGTGTGTGTTTGTATTTCTGTATGATCTCTACTTTCAGTGTAGTCATCATCTCTTACTACTTACTAGTGTGTTTGTATGATGTGAGAACTTGAAAATGGGTTAGTCTTCCTTTGGATTTGTATTTGGTTTGGGGTTGAAAAAACACTACGTTTGGCTTTTGTAGTTTTAGTTAATGGAATAATAATGTGATAACAAGTTTGCTTCATATATTACTATATTGTTCATCTTGGGAGCAACATTTCTTAAAAATCTCAAATGTTTATGGTTGTGTT
TARGET=574,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00258 Ps_contig_mira-and-tgicl-ass_franssen_3012
SEQUENCE=GGSCTCACATCACAATACACAAATACACGAATACACATACACTCTCTCTCTCTCTCCCTCTTTCTCAATCCGCGTTATTACCCTAAAAACAACAAATTCTTCAATCGAATCCAACAATGGCTCTGCGAGTAACAACCCCTTTCCCTACTCAAACTTCCTCCTTCTCCCTCCCTCAAATGGCCAGTCTCAGATCCTACCCAAATTCGTCATGGCCTTCCCACCCTCCGCTCCGGTTACCAAAAGGAGGGTTTAG
TARGET=42,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00259 Ps_contig_mira-and-tgicl-ass_franssen_3019
SEQUENCE=CTTTTTTTTTTSGKAATATTAATAAACGAATATATCATTTAAAAATAAAAGTCAAGCAAAATATATGTGTGTAGGATATGCATGTGTAGTTACTTGTCCTTTGTGAGACAAATGTACATGCTATAGGAATATGACAGTAATACGATACAAAATATCTTTTTCAGTCGTAATCTTAGTTATAAAATGGCATGGCATGGCAGCCTTACAAAATATATCTTCACGACCAAACAAAATCCCATATCCATCAA
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00260 Ps_contig_mira-and-tgicl-ass_franssen_3023
SEQUENCE=GCTATTTCCCTTGGGGAGAATGATATCAAGTTAAGTTATTTCCTTCCCTTGACACCTGAATCTTCAAGCTCTGATTGGTAGCTGATAATTCGTGGCTTATAGGGTCTGATATGGTTGTTGTTGTTGTTAATAAATGAGTTGTTCATCAGMCCAAATCTACGAGGCTTAGGGCAAACCACAGATTCCATCCTCATCTCTTACACAGCTTCTCATGGCGTTCTTCTGATGGTAACCACAGTGATTCATTCTTTCTCTCTAATTTCACACCTAGAAAACCAAAGATACTTAAACAAAGAACACCTGGAAGGTAAGCTAGAATCAGAGACCTCCTACGTAAAGGAAGGGAAGGAAAAGGAAAGGGAAAAAAATACG
TARGET=114,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00261 Ps_contig_mira-and-tgicl-ass_franssen_3025
SEQUENCE=GGTTTTTCCGTATTTTTTTTTCCCCCCTTTTTTCCCTTTCCCTCCTTAGTAGGAGGCTCTGATTCTAGCTTACCTTCCAGGTGTGAAAATTAGAGAGAAAGAATGAATCRCTGTGGTTACCATCAGAAGAACGCCATGAGAAGCTGTGAAGAGATGAGGATGGAATCTGTGGTTTGCCCTAAGCCTCGTAGATTTGGTCTGATGAACAACTCATTTATTAACAACAACAACAACCATATCACGACCCTANAAGCCACAAATTATCAGCCTACCAATCAGAGTCTTAGAAGTATTCGAGGTGTACGAAGGGAANTTCCTTTCGTTTAGTAA
TARGET=220,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00262 Ps_contig_mira-and-tgicl-ass_franssen_3035
SEQUENCE=GTCGGGACCTACAAAAACAAACTCACGCTCTCTCTCTCAARGGGGATTCATTCCGCCTCAAACCCTAGCCGCCGCTAAACCCTTTTCCTCTTCTCTTCAACAACCGTCAAAACCCTAAATCTCTCTCTTTCAAACCGCGAGCTCGCCGCCATCACCACCGGCGAAAACCCCAACGAACACCATACAACCTCCACGCCGTAACATCTAAACCATCAATCGTCTTCCCCATTTCATCCGCCGAAATACACCGATTAACCTCCGCCTTGACCAAAAACCCCCAAATCCTCACTTCATTTCGTCCCTTTCGAAATTA
TARGET=28,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00263 Ps_contig_mira-and-tgicl-ass_franssen_3041
SEQUENCE=GAAGCAGTGGTAGTCAACGTCGGGGGCTAATTTCCATTTTGTTAGTTCGGTTTAGGTGGAGAGAGAGAGAGAGTGTGTTGAGATTTTTTCTTTCATTTTTCTTATCAATTTCTCTTTATTTGTTAGGTTGTTTTTTCTCTTTCCTCATTTTTGTAGGTATATTGTGGAAGAAGACTTTGTAGCTTGACTTTGTTCAAAAGCATACGATTCAGGGAAGGCCGTAAAGTTGGAGTGAAATTAGATTGAAATCTTAGAAGTTTCTCTCTTTGGATTTGAAGAAATGGAGGTAYATGGAAATTCTATGGTTGCCGCTCCTTCAAATGTTATTTATCTGTCAAGTAT
TARGET=59,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00264 Ps_contig_mira-and-tgicl-ass_franssen_3043
SEQUENCE=TCACACAAAAACCCTACAACAAGTTTAAGACAAAAAAAAGTAGATTTTTTTGACCAAAAAAATAAGAGGAGAATCYTCGTTTTTTATAGAAGCCGGAAAATTAAAAAAATWAAATTAAAAATGTAGAATTTACATAAGGAGAAATGTGATCAAGCGTTTTTATGAGTGCCCCAGTTTGAGAAAAACCGGATAGGATCAAAATCGGAGTCAATTAAAATATATATTCTGAAAATTGTTTTATAAAATATACATTGAACCAGAAATGGAAACAAAGGGGATTAGGGTTTTTAGATGGATCATCAGAGAACAAACAACAAACGGAGAGAGAGTGAGAGGGAGAGAGAGAAGAGTTGGTGTTGTTGGAATAACAAAGGCTTTGGCTTTCGCTTACACTCTCTTGGCCTTTCTTTGTGTTGTC
TARGET=337,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00265 Ps_contig_mira-and-tgicl-ass_franssen_3054
SEQUENCE=CCGACTCCTTAAGTAGTTTTCCGGTCTCAGAATCCCAAATACTCATAGAGACATTCAACTTCATTCACTGTCACAGTAAAGAGTATTTCTAATTCCGATCAACGAAGATAAACATCAAACACATCAAAACCATCCACATCTTAGCGCTTCTCTTTCCCTGATTTCGCTCCATCACTTCTCCCCTTTTTATTTATTTAATTTGGGGTTTTTTTCTGGAATTGAAAAAAAATCGATAATTGTSTATTGCAGNTCTAAGNTTGGATTTGAGAGGTTTNACTTNCGTCATGCAGTNCACGCAACAACGNAT
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00266 Ps_contig_mira-and-tgicl-ass_franssen_3055
SEQUENCE=AGGACGTCCACGAAAATAAATACATGGTCAACAACAACAAAATTACCCCGAAACCAAGTGTCCCCTCCGTAAAGCACACACTCATAGAGACATTCAACTTCATTCACNGTCACAGAAAGAGATTCAATCCGATCAACGAAGATAAACATCAAACACATCAAAMCCATCCACATCTTAGCGCTTCTCTTTCCCTGATTTCGCTCCATCACTTCTCCCCTTTTTATTTATTTAATTTGGGTTTTTTC
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00267 Ps_contig_mira-and-tgicl-ass_franssen_3055
SEQUENCE=AGGACGTCCACGAAAATAAATACATGGTCAACAACAACAAAATTACCCCGAAACCAAGTGTCCCCTCCGTAAAGCACACACTCATAGAGACATTCAACTTCATTCACNGTCACAGAAAGAGATTCAATCCGATCAACGAAGATAAACATCAAACACATCAAAMCCATCCACATCTTAGCGCTTCTCTTTCCCTGATTTCGCTCCATCACTTCTCCCCTTTTTATTTATTTAATTTGGGTTTTTTC
TARGET=220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00268 Ps_contig_mira-and-tgicl-ass_franssen_3070
SEQUENCE=GAAGGCAGTGACAGATCGACTTTATCAGATTTTTTTGTTGTAACCCCTTATATTTATTTATCCTTCCGTAGATAGTCGGGGAATGAGAGAGAGAGAAGAACAAGGGTGACTCTCATTCCCCAAACCCTATTCCTCTCTTTCCGTTTCCACGCTTTCTCTTCTCTGACTTTCATTTCAGGATTAACGCCGTCGTTTCGCCGCCGATCATCGTCTGATTAAGAGCAGTGAAGAATAAACTGATGGCTAACATGGATGGCAAATCACTCCTGAGTCCTAAGAATTCCATTCCCATACCCCCTCCTTCGCCTCAATCAGCAGCAACAGGTTATCCTTGCGAGTATGTGGTGATGAATAATTTAGCACCCAGACGGCGAAGCAGTTGTGTCCCTACTGCAACAAGAGATTTGTGGGAGCGTCTTTTCAATGAAGGTTATAAAGCCAATGTTCGCA
TARGET=85,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5499
PRIMER_LEFT_PENALTY=0.519744
PRIMER_RIGHT_PENALTY=0.030114
PRIMER_LEFT_SEQUENCE=TATCCTTCCGTAGATAGTCGGG
PRIMER_RIGHT_SEQUENCE=GGCGTTAATCCTGAAATGAAAG
PRIMER_LEFT=58,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=59.480
PRIMER_RIGHT_TM=59.970
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.6780
PRIMER_LEFT_1_PENALTY=0.519744
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=TATCCTTCCGTAGATAGTCGGG
PRIMER_RIGHT_1_SEQUENCE=AGGATAACCTGTTGCTGCTGAT
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=331,22
PRIMER_LEFT_1_TM=59.480
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=0.6906
PRIMER_LEFT_2_PENALTY=0.519744
PRIMER_RIGHT_2_PENALTY=0.170825
PRIMER_LEFT_2_SEQUENCE=TATCCTTCCGTAGATAGTCGGG
PRIMER_RIGHT_2_SEQUENCE=GAGTGATTTGCCATCCATGTTA
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=59.480
PRIMER_RIGHT_2_TM=59.829
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.6930
PRIMER_LEFT_3_PENALTY=0.675181
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=CTTCCGTAGATAGTCGGGGAAT
PRIMER_RIGHT_3_SEQUENCE=CACCACATACTCGCAAGGATAA
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=60.675
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.7053
PRIMER_LEFT_4_PENALTY=0.675181
PRIMER_RIGHT_4_PENALTY=0.030114
PRIMER_LEFT_4_SEQUENCE=CTTCCGTAGATAGTCGGGGAAT
PRIMER_RIGHT_4_SEQUENCE=GGCGTTAATCCTGAAATGAAAG
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=187,22
PRIMER_LEFT_4_TM=60.675
PRIMER_RIGHT_4_TM=59.970
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00269 Ps_contig_mira-and-tgicl-ass_franssen_3079
SEQUENCE=GTTCGGGGGGKCAGAGATTGGTTTGGTTAACGAACGCTTCAACGGCAAAATTTCCCGGGCTACAAGGCTTCCTGTCTCTCTCTCTCTTTCATCTCCATGCGCGACAATTCCCATTTTCTCACTCATAATTTCCATCTCCTCCAAAAGGATGCGTAATTGGAAGGGGGATTGGCAATAACTGGAGGAGGAGAAGGTAGGGAGGTGTGGTGGGTGAGAGTGAGTTGGGTCTAAGGTGAAGNT
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00270 Ps_contig_mira-and-tgicl-ass_franssen_3082
SEQUENCE=AATAGTTTGAGTCGAACCACCGGTAATAGCTTTCTTTTTTAAGTTTCTCCCAGTTTAGCTTCCACCTTCTCTTTAACAGGGGCGGGAACTTTCYTCTCCTAGCTCTTTCAATTGCTTCTCAGTCTGGTAGACAACAGAGTCTGCCTGGTTCTTGGTGTCGATTGCTTCCCTCTTTTCTTTGTCCTCTTTTGAAAATCTCTCTGCCTCGCTCACCATTCTCGACAACATCATCTTTTGCCCATATAAACAAAACAAACAAACAAAAAAACCAA
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00271 Ps_contig_mira-and-tgicl-ass_franssen_3095
SEQUENCE=CGTCGGGGACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACACCACAACTCACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACAGTTAGCTCAGAAGAACTCATCCAAAATGGTTATCTCATCAACTGTCAAACATCCAGATGAACATAGTGAAAGCATAGACTGTACTTTTGCTTCTAGATACGTGCGTGAAGCAGTTCCAAAGTTCAAGCTGTCAGAGAGTTCTATACCAAAAGATGCAGCATATCAGATAATAAACGATGAGTTGATGTTAGATGGGAAACCAAGGCTTAACTTGGCTTCATTTGTCACTACTTGGATGGAACCTGAGTGTGATAAACTCATCATGGATTCGCTTAACAAGAACTATGTTGACATGGATGAGTATCCTGTCACCACTGAGCTTCAGAATCGGTGTGTGAATATAATAGCACATCTATTCAATGCTCCTATTGGTAAAGAGGAAACAGCAGTAGGTGTTGGAACCGTAGGATCATCAGAAGCAATAATGTTAGCTGGTTTAGCTTTTAAAAGGAAGTGGCAAACTAAGAGAAAATCAGAAGGCAAACCTTACGATAAGCCCAACATCGTTACCGGGGCTAATGTGCAGGTTTGTTGGGAAAAATTTGCTAAGTACTTTGAGGTTGAACTAAAGGAAGTGAAACTCTCGGAAGGATACTATGTGATGGATCCATTGAAAGCAGTTGAAATGGTAGATGAGAACACTATCTGTGTTGCAGCCATTTTGGGTTCAACGCTGACCGGAGAGTTTGAGGATGTGAAGCTTCTTAACGAACTTCTTACTGTAAAGAACAAAGAGACGGGATGGGATACGCCGATTCATGTAGATGCTGCGAGCGGTGGTTTTGTTGCTCCGTTTCTCTACCCTGATCTGCTATGGGATTTTCGCTTGCCATTGGTGAAAAGTATCAATGTTAGCGGTCACAAGTACGGTCTTGTTTATCCCGGTATTGGTTGGGTTGTCTGGAGGAATCAAGATGACTTGCCGGATGATCTTGTTTTTCATATCAATTATCTTGGATCTGATCAGCCAACTTTCACATTGAATTTCTCCAAAGGATCGAGTCAAATCATTGCTCAATACTACCAATTCATACGACTTGGATTCGAGGGCTACAAAAGTGTGATGGAAAATTGCGTTGGACGAAACACGGAAGTAATTCGTAAGGAAAAAGGAAAGGGTAATTTAGGAAAGTAAAACGTTAGGGTTAAAAGTTTTAAGTATTTGGTACGTACCGAAAGGGGTACCGGTTCCGGG
TARGET=39,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3776
PRIMER_LEFT_PENALTY=2.347318
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT=1,20
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=2.3783
PRIMER_LEFT_1_PENALTY=2.347318
PRIMER_RIGHT_1_PENALTY=0.030936
PRIMER_LEFT_1_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_1_SEQUENCE=AACTGCTTCACGCACGTATCTA
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=59.653
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=2.6910
PRIMER_LEFT_2_PENALTY=2.347318
PRIMER_RIGHT_2_PENALTY=0.343680
PRIMER_LEFT_2_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_2_SEQUENCE=TCTCTGACAGCTTGAACTTTGG
PRIMER_LEFT_2=1,20
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=59.653
PRIMER_RIGHT_2_TM=59.656
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=2.6910
PRIMER_LEFT_3_PENALTY=2.347318
PRIMER_RIGHT_3_PENALTY=0.343680
PRIMER_LEFT_3_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_3_SEQUENCE=CTCTGACAGCTTGAACTTTGGA
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=59.656
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=2.7599
PRIMER_LEFT_4_PENALTY=2.347318
PRIMER_RIGHT_4_PENALTY=0.412596
PRIMER_LEFT_4_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_4_SEQUENCE=GCTTGAACTTTGGAACTGCTTC
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.413
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_00272 Ps_contig_mira-and-tgicl-ass_franssen_3095
SEQUENCE=CGTCGGGGACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACACCACAACTCACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACAGTTAGCTCAGAAGAACTCATCCAAAATGGTTATCTCATCAACTGTCAAACATCCAGATGAACATAGTGAAAGCATAGACTGTACTTTTGCTTCTAGATACGTGCGTGAAGCAGTTCCAAAGTTCAAGCTGTCAGAGAGTTCTATACCAAAAGATGCAGCATATCAGATAATAAACGATGAGTTGATGTTAGATGGGAAACCAAGGCTTAACTTGGCTTCATTTGTCACTACTTGGATGGAACCTGAGTGTGATAAACTCATCATGGATTCGCTTAACAAGAACTATGTTGACATGGATGAGTATCCTGTCACCACTGAGCTTCAGAATCGGTGTGTGAATATAATAGCACATCTATTCAATGCTCCTATTGGTAAAGAGGAAACAGCAGTAGGTGTTGGAACCGTAGGATCATCAGAAGCAATAATGTTAGCTGGTTTAGCTTTTAAAAGGAAGTGGCAAACTAAGAGAAAATCAGAAGGCAAACCTTACGATAAGCCCAACATCGTTACCGGGGCTAATGTGCAGGTTTGTTGGGAAAAATTTGCTAAGTACTTTGAGGTTGAACTAAAGGAAGTGAAACTCTCGGAAGGATACTATGTGATGGATCCATTGAAAGCAGTTGAAATGGTAGATGAGAACACTATCTGTGTTGCAGCCATTTTGGGTTCAACGCTGACCGGAGAGTTTGAGGATGTGAAGCTTCTTAACGAACTTCTTACTGTAAAGAACAAAGAGACGGGATGGGATACGCCGATTCATGTAGATGCTGCGAGCGGTGGTTTTGTTGCTCCGTTTCTCTACCCTGATCTGCTATGGGATTTTCGCTTGCCATTGGTGAAAAGTATCAATGTTAGCGGTCACAAGTACGGTCTTGTTTATCCCGGTATTGGTTGGGTTGTCTGGAGGAATCAAGATGACTTGCCGGATGATCTTGTTTTTCATATCAATTATCTTGGATCTGATCAGCCAACTTTCACATTGAATTTCTCCAAAGGATCGAGTCAAATCATTGCTCAATACTACCAATTCATACGACTTGGATTCGAGGGCTACAAAAGTGTGATGGAAAATTGCGTTGGACGAAACACGGAAGTAATTCGTAAGGAAAAAGGAAAGGGTAATTTAGGAAAGTAAAACGTTAGGGTTAAAAGTTTTAAGTATTTGGTACGTACCGAAAGGGGTACCGGTTCCGGG
TARGET=113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4258
PRIMER_LEFT_PENALTY=0.395499
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=ATCTTCTTCTCTCCCACACCAC
PRIMER_RIGHT_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT=55,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=59.605
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.4264
PRIMER_LEFT_1_PENALTY=0.395499
PRIMER_RIGHT_1_PENALTY=0.030936
PRIMER_LEFT_1_SEQUENCE=ATCTTCTTCTCTCCCACACCAC
PRIMER_RIGHT_1_SEQUENCE=AACTGCTTCACGCACGTATCTA
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=59.605
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.6258
PRIMER_LEFT_2_PENALTY=0.395499
PRIMER_RIGHT_2_PENALTY=0.230329
PRIMER_LEFT_2_SEQUENCE=ATCTTCTTCTCTCCCACACCAC
PRIMER_RIGHT_2_SEQUENCE=TCTGATATGCTGCATCTTTTGG
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=59.605
PRIMER_RIGHT_2_TM=60.230
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.6586
PRIMER_LEFT_3_PENALTY=0.628294
PRIMER_RIGHT_3_PENALTY=0.030276
PRIMER_LEFT_3_SEQUENCE=GATCTTCTTCTCTCCCACACCA
PRIMER_RIGHT_3_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=281,22
PRIMER_LEFT_3_TM=60.628
PRIMER_RIGHT_3_TM=60.030
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.6586
PRIMER_LEFT_4_PENALTY=0.628294
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=TGATCTTCTTCTCTCCCACACC
PRIMER_RIGHT_4_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.628
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_00273 Ps_contig_mira-and-tgicl-ass_franssen_3096
SEQUENCE=TTTCGCTAAGGAAACAAGACAAGTCTTATTCATTCATTCATATAAATATATGGTTTAGAGATACATTGACAATGGTTATATCTCTACATAGATAAATAAAGTAACAATGAAACAATCCTTACACACATTAAATTTAATTGTACCGCACAAACAAAGTAGCTCACAAAACACAACAAACATTTGACAACACTAGCATGTTCCAACTCTCTTTCCTGTAGCAAGCTTCTTCCAATAACTTGCAATCTCAGTTTGAGTCTCTGTAATATCTTTCTTAACCTCCTCGCTTGTCTCACTCGCGATAACCGCGACATGAGCACTTTTCGAGGAGATGGTACTTGGAATTGTCTCCAAGAGAGCC
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3172
PRIMER_LEFT_PENALTY=1.302444
PRIMER_RIGHT_PENALTY=0.014720
PRIMER_LEFT_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_SEQUENCE=CAAGCGAGGAGGTTAAGAAAGA
PRIMER_LEFT=1,22
PRIMER_RIGHT=286,22
PRIMER_LEFT_TM=58.698
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=1.3213
PRIMER_LEFT_1_PENALTY=1.302444
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_1_SEQUENCE=GAGACAAGCGAGGAGGTTAAGA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=290,22
PRIMER_LEFT_1_TM=58.698
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=1.3236
PRIMER_LEFT_2_PENALTY=1.302444
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_2_SEQUENCE=GGAAGAAGCTTGCTACAGGAAA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=229,22
PRIMER_LEFT_2_TM=58.698
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=1.3895
PRIMER_LEFT_3_PENALTY=1.302444
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_3_SEQUENCE=ATTGGAAGAAGCTTGCTACAGG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=58.698
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=1.4102
PRIMER_LEFT_4_PENALTY=1.302444
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_4_SEQUENCE=GACAAGCGAGGAGGTTAAGAAA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=58.698
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_00274 Ps_contig_mira-and-tgicl-ass_franssen_3123
SEQUENCE=ACAACAACTGTTTACTTATTTATTTATACATTAACATTTCAGGATTACATGACCATATAACTTCTTGCACACCCACAAAAGGAAACATTATAACCAAGAAAGCACACGATTTAAAGCAACAGAGTAACAGACTCAGTCTAAGTTTAACCAGAAATCTCAATAGACTTAACCTCAGCCTTCTTTATCTCTTTCTTTTGGAACTGTCACTGTGAGAACGCCGTTCNCCATGGGAAGCTTTCACTTTATCCTATTTTTA
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00275 Ps_contig_mira-and-tgicl-ass_franssen_3126
SEQUENCE=ATTGTCTAACCTTTCTTGTTTCATCAAGCGCTATTTTTGTACTTATGTTCATCATTTGATATCTTGGTCTCTGGAATTACATCATTGAGGTGGACTTTTTAAGGACTATAAAGTACAGAATGGTAGGTACACATCATACAACATGCGCCCACCGGTTATTCTCGAACCATCCGCCACCTTTTCTTCTTCTTCTTCTCCAACCGATCGCAGGCTAAACACCCTCGCAAGACACCTTTCTCAATTTCCAGATCATAATCAAATGGCTTCTCATATTTCTCCTTCTCCAACAGCTTCTTCCGATTCCGTTTTCGCTCACATCGCTCAAGCCTCCCGAAGATCCTATCCTYGGGGTAACTGTTGCTTACAACAAAGATCCAAGTCCTAWTAAGCTCAACTTGGGCGTTGGTGCTTACCGAA
TARGET=181,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00276 Ps_contig_mira-and-tgicl-ass_franssen_3127
SEQUENCE=GATAAAGTAGGCGTACAGAAACCACACCTTTTCKACTTTACATTTTTTTAGTTAACTTTTAATTTAAATTTATTCAACCAAAACCAAACCAGAAAATTCATACACAGAATATATATACACCACCCCATCATACAACATGCGCCCACCGGTTATTCTCGAACCATCCGCCACCTTTTCTTCTTCTTCTTCTCCAACCGATCGCAGGCTAAACACCCTCGCAAGACACCTTTCNCAATTCCAGATCATAATCAATGGCTTCTCATATTCNCCTTCTCCAC
TARGET=175,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00277 Ps_contig_mira-and-tgicl-ass_franssen_3128
SEQUENCE=TCGGGKACCGGTTATTCTCGAACCATCCGCCACCTTTTCTTCTTCTTCTTCTCCAACCGATCGCAGGCTAAACACTCTCGCAAGACACCTTTCTCAATTTCCAGATCATAATCAAATGGCTTCTCATTATTTCCTTCCCTTTCCTACCCAAACAGTCCTTTCCTTCCGTATTTCCGTTTTCGCTCACNTCGCTCAAG
TARGET=37,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00278 Ps_contig_mira-and-tgicl-ass_franssen_3129
SEQUENCE=ACCAACGCCCAAGTTGAGCTTAATAGGACTTGGATCTTTGTTGTAAGCAACAGTTACCCCGAGGATAGGATCTTCGGGAGCTTGAGCGATGTGAGCGAAAACGGAATCGGAAGAAGCTGTTGGAGAAGGAGAAATATGAGAAGCCATTTGATTATGATCTGGAAATTGAGAAAGGTGTCTTGCGAGAGTGTTTAGCCTGCGATCGGTTGGAGAAGAAGAAGAAGAAAAGGTGGCGGATGGTTCGAGAATAACCGGTGGGCGCATGTTGTATGATGGGGTGGTGTATATATATTCTGTGTATGAATTTTCTGGTTTGGTTTTGGTTGAATAAATTTAAATTAAAAGTTAACTAAAAAAATGTAAAGTCCCCGAGTASTSTGGTTTCTGTACGCCTACTTTATCCCCGNGTA
TARGET=211,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00279 Ps_contig_mira-and-tgicl-ass_franssen_3130
SEQUENCE=CTCCTTTGTTGCGTAAAGAAACAAAGTCGTTCAAACAGTTGGTGTTCAACACGCCTCACTACATCCAACACAAGGGGTTTACCTTCCTCAGTTCGGTAAGCACCAACGCCCAAGTTGAGCTTAATTAGGACTTGGATTCTTTTGTTGTAAGCAACACGTTACCCCGAGGATAGGATCGTTCGGGAGCTTGAGCGATGTGAGACGAAAACGGAATCGGAAGAAGCTGGTTGGAAGAAGGAAGAAATATGAAGAAGCYATTTGATTATGATCGTAGGAAATTGGAAGGTAAAAGGTTGTTCTTGGCGAGGAGTTGTTTTACGCCCTGGCGAGTTCGGGTTAGGAAGAAAGAAAGAAAGAAAGGAAAAGGGTGGACGGAGT
TARGET=342,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00280 Ps_contig_mira-and-tgicl-ass_franssen_3143
SEQUENCE=TTAGGGTCAAGGAAAAAGGAAGAAAGTAATGGATGCTCGAGGACGTCATTACCAGAAAGGAGGAAGACAAAGNTCCACCGATTGTGTCATTAAGAAGACCCTACCGAACGGGAGTGATTGTAAAGAGCTCCGAACGAAGTAGGGTACGATGAGTCTGTGATGTAGAGAGTGAATGTGTTTGTATAGGAACTTGTTCTGATGCATGTACCTGTGCATAAAGTATTGCCATGTGTCCATTACCAACATAGCAGTAACAAATTGTCTTGCTAAAACAAGAATAGAAGCATTCTCATTGGTACTACTTTGTGTATCACTTCCTGTCACTGCAAATAAGAGGGTTGCAACCACAGCTTGAACAGCTTGTTGAAGGAGAACTCCTTTAACCACATCAAACTTAGAAACCAAATTCTTTTCATCCTCATCTTTTATTGTATGTAACCTATAATCTTCAGCAAACAACCCCAAAACAACGTAAAAACCAGAATAAATCCAATAAACTAAAAGTGGAGCAAAAGTCCCCAACATCTCATCAGAAATCTCCAAACCCATTTTCTCACACACCCAGAAGAAAAAATCAAATTTTTTTCTCCGATTTGATTTAGGTACAACCCACAAACCCCAAAATTTGCATGAATTTCAAAGTACCCAAAGAAACAAAAGGATTTAATAACAAAATTGGAAACCCTTAACCCTAACCCTCTTTATGGAAAATTTATAAAAAAAAAACCTTAAAATTGAAACTCTCCCTCTTCTTGTTCCTCTAACCCTAATGATTGAATTCAAACCCAAAACCAATAACAACGCGATTAAATATTGGATTAGGTTTCCAGGAAGGTTCCAAAGAGAGAAGAGAGAGAGAATGTTGAAAACTGTTTTTCCT
TARGET=849,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00281 Ps_contig_mira-and-tgicl-ass_franssen_3144
SEQUENCE=ATTTCCTCAAGGACCCTGTGAAATACGATGTTAAATATCAGGCTAGTGCACAACCGTTTTTGGTTCTTGGTCTGTTGGTTACTGCTCTAGCAACAAGCCATTGATAAAAAGAAGTTCAAGAGAGAGAGAGTCACACCAGTTAGAAATAATTTCATAAATTTGTATTGAAGATGTGTGCCATAGTATTAATGTAATTTCATTTTTCTTGTTTTTTCCTTCTAATTTTTGTTTTGTTCTATCTTCTCTTATCCACTTAATTTTTTTTAAAGTATTTCTAAGCTCAGGTTGAGAAAAGATTTATTTTTAGGAAACTATAAATAAGACCAATTGTACAAATGTATTGTAGACATATTCGCATATACAATTTTCCTTCCATAGTTYAATAAATAATTAAAACATTGGCTACCTATCATGGTTTTATTAAGTT
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00282 Ps_contig_mira-and-tgicl-ass_franssen_3157
SEQUENCE=ACAAGAGGGTGCCCTGGGAACAAAGAAGCAACGTTKTSCCYATTCAGGGCCCATGCCAAGCAACATGAGATCAAATTTTGGAAATCCACTGGTTGATGATGATGTTATCACATTGCTATTGACCAAGTGTTTGACACATGTCTCGTAATCATCAGCTGCTCCTTCAGCTGGAAAGGGCTCATTGATTGACAATAAACAGTTCGGCCCGGGGTTAGGGAAAGGTTCGGGGGTAACCCGTTTGGGGAAGGAAGGAAAACGGCCCAA
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00283 Ps_contig_mira-and-tgicl-ass_franssen_3178
SEQUENCE=AGKAAAGAAGCAAAGATTCATAGTTCATTGCAGAAAATTTCATAATAAAACAACAGAAACAATTGAAAGAAAGTAAAAATAATGTTACAACTTAAGAGTTTCGCAAATAAGGATAGAAAAACCTTTTACTGAAAATGTTTAGAAGTGATTGAACATTATACATAGCAATGGCACATTTAAAGTGATACAGATACTAGTACATAACCACTAAAGCACAACCAACCCACAAGAAGCATCAGGAGTTGTTCCTTTTGCGAGCTATCTCCCTCGCCATCTCTCTAAGCTCCTCTGGTGGTGGTGGCTTTTGTGTATCTTTGGGATATTCAACGTATGATTTTTTTCCGATGAACTTGTGGAGGGTGTTGTCGGTATTGCTAGCATAAACGTACATAAGAACGATGGGTACAGTAACGTAAACCCCAAACTTGGCGATCTCTAGAATCCCCTTTGAAGTTCCCAGTGATGACATCTTTCCTTTCCTTCCGCCAACAAACTCAACTAACAACTCAGATCAAATCACACACCTCTCAATTTAGATCAAACCATATGGGTGTTGCAATAAATTAAATTGACCCAGCCAGTTGAATTGAGAGGATAGTGTGAACCTAATATGACCTCTGCTCCCGGTATCTCACGTGTTCCACGTGATTATACTATAGTGGACCGAGATCCCCGAC
TARGET=290,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00284 Ps_contig_mira-and-tgicl-ass_franssen_3183
SEQUENCE=TAATATCGGGACAATCAGGTGCCGTTGATCGATCGATCTGGGAGAAGAATGGGAGCAGGAAAGAATACGGCAGAGTTCTTCAGGAGGAGAGACGAATGGAGGAAGCATCCGATGCTTGGGAATCAGTTTCGTCACGCTACTCCTGGCCTCGGTATCGCTCTCGTTGCGTTTGGTGTTTACCTCGTCGCTGAGCAAGTTTACAACAAGGCCAATGCTTCTTCCTCTTCTCACCATGTGAAGGCGTGGGGAGCACTGAAGGAAAATCAACTAATTCATCTATTTTCCTTTGTTTTGTTTTGGTTTAAGTTGTTTAGTTAAATTTAAACGG
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1195
PRIMER_LEFT_PENALTY=1.057682
PRIMER_RIGHT_PENALTY=0.061818
PRIMER_LEFT_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_SEQUENCE=AGCATTGGCCTTGTTGTAAACT
PRIMER_LEFT=0,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=61.058
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=1.1671
PRIMER_LEFT_1_PENALTY=1.057682
PRIMER_RIGHT_1_PENALTY=0.109391
PRIMER_LEFT_1_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_1_SEQUENCE=CTTGTTGTAAACTTGCTCAGCG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=61.058
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=1.1743
PRIMER_LEFT_2_PENALTY=1.057682
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_2_SEQUENCE=GTTGATTTTCCTTCAGTGCTCC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=61.058
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=1.2137
PRIMER_LEFT_3_PENALTY=1.057682
PRIMER_RIGHT_3_PENALTY=0.156061
PRIMER_LEFT_3_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_3_SEQUENCE=TCACATGGTGAGAAGAGGAAGA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=61.058
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=1.4399
PRIMER_LEFT_4_PENALTY=1.057682
PRIMER_RIGHT_4_PENALTY=0.382228
PRIMER_LEFT_4_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_4_SEQUENCE=ATGGTGAGAAGAGGAAGAAGCA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=61.058
PRIMER_RIGHT_4_TM=60.382
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_00285 Ps_contig_mira-and-tgicl-ass_franssen_3185
SEQUENCE=GAGAGAAATTTATGATAAATGGTAGAATTAATTTTGATTTAAAATCAATAACATTTTATGGAGTTATTATTATATGCTGGCGGGAGAATCTGTCCAGATTTGGAAGAGAGAAAAAAAAGTTTATTTTTATTTATTTATAAGTAAAATTTTGTTGATTCCATAAAAAAAATTAGTTTATGGTGTTTGTTGTGCACATGGTTTGGTTTCGAATTGTTATTTGCAATGTGAAAGGTAGGTGTTGTTTTTTTTAACCTT
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5153
PRIMER_LEFT_PENALTY=0.177514
PRIMER_RIGHT_PENALTY=0.337818
PRIMER_LEFT_SEQUENCE=ATTATTATATGCTGGCGGGAGA
PRIMER_RIGHT_SEQUENCE=CATGTGCACAACAAACACCATA
PRIMER_LEFT=65,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=59.822
PRIMER_RIGHT_TM=60.338
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.6380
PRIMER_LEFT_1_PENALTY=0.177514
PRIMER_RIGHT_1_PENALTY=0.460467
PRIMER_LEFT_1_SEQUENCE=ATTATTATATGCTGGCGGGAGA
PRIMER_RIGHT_1_SEQUENCE=CAACACCTACCTTTCACATTGC
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=59.822
PRIMER_RIGHT_1_TM=59.540
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.9284
PRIMER_LEFT_2_PENALTY=0.177514
PRIMER_RIGHT_2_PENALTY=0.750851
PRIMER_LEFT_2_SEQUENCE=ATTATTATATGCTGGCGGGAGA
PRIMER_RIGHT_2_SEQUENCE=AAACCATGTGCACAACAAACAC
PRIMER_LEFT_2=65,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=59.822
PRIMER_RIGHT_2_TM=60.751
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=1.0955
PRIMER_LEFT_3_PENALTY=0.757635
PRIMER_RIGHT_3_PENALTY=0.337818
PRIMER_LEFT_3_SEQUENCE=TTATTATATGCTGGCGGGAGAA
PRIMER_RIGHT_3_SEQUENCE=CATGTGCACAACAAACACCATA
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=60.758
PRIMER_RIGHT_3_TM=60.338
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=1.2181
PRIMER_LEFT_4_PENALTY=0.757635
PRIMER_RIGHT_4_PENALTY=0.460467
PRIMER_LEFT_4_SEQUENCE=TTATTATATGCTGGCGGGAGAA
PRIMER_RIGHT_4_SEQUENCE=CAACACCTACCTTTCACATTGC
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=240,22
PRIMER_LEFT_4_TM=60.758
PRIMER_RIGHT_4_TM=59.540
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_00286 Ps_contig_mira-and-tgicl-ass_franssen_3237
SEQUENCE=ATTTTTAGCTCAAAGGAAGAAGGTTCTTGAGGGAAAGGAGTGAAGTGGGTTTTGTAACGAGTTTTGTTGGGTTGTGGAACTTTGGTGAACTAGGTAGTGTTGAAGCACTTTGTGTTCATCATGGTCATTGCGATTATGGTTGATGCCATTGATTGATTGATGAATGAGTAGAAGTGTCTTTGTTTGAGNGTGTAGAATGATGTGTGGTNTTTGCGTAGGTTAGG
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1400
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.102638
PRIMER_LEFT_SEQUENCE=AAGCACTTTGTGTTCATCATGG
PRIMER_RIGHT_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT=102,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.1679
PRIMER_LEFT_1_PENALTY=0.065292
PRIMER_RIGHT_1_PENALTY=0.102638
PRIMER_LEFT_1_SEQUENCE=TGTAACGAGTTTTGTTGGGTTG
PRIMER_RIGHT_1_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.935
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.2046
PRIMER_LEFT_2_PENALTY=0.102004
PRIMER_RIGHT_2_PENALTY=0.102638
PRIMER_LEFT_2_SEQUENCE=AAGAAGGTTCTTGAGGGAAAGG
PRIMER_RIGHT_2_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=221,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.2046
PRIMER_LEFT_3_PENALTY=0.102004
PRIMER_RIGHT_3_PENALTY=0.102638
PRIMER_LEFT_3_SEQUENCE=AAAGGAAGAAGGTTCTTGAGGG
PRIMER_RIGHT_3_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=0.2448
PRIMER_LEFT_4_PENALTY=0.142170
PRIMER_RIGHT_4_PENALTY=0.102638
PRIMER_LEFT_4_SEQUENCE=TTCTTGAGGGAAAGGAGTGAAG
PRIMER_RIGHT_4_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00287 Ps_contig_mira-and-tgicl-ass_franssen_3255
SEQUENCE=TTTAGGAAATACTCTCCTGGCCAGCTGTATCCCAAATCTGTAAGGTGAAAAGCCTATCTTCAAATTGCACTTCTTTGGTTAAGAAATCCGCTCCAATGGTTGCCTTGTACTGATTACTAAACTTCTTAATTCACATATTGGTTCATCAAAGACGTCTTCCCCACACCGCTGTCACCGAGAATGATGACCTTTAAGAGAGTTCTTCTGCGAGAAGGCATAGTACGGATCGGTGGAAGAAGAAGAAACTGTTTGCTTGTGTTTGATGATTCTTTTTTTACTTCAACTAGGACTCTCTCACACCGTCTTCAACTCTATTTCTCTCTCTCTAACTTTCTAAGCAGCCGYCTTTCTCTTTCTATCTACAAATTCTTGAGRACGG
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00288 Ps_contig_mira-and-tgicl-ass_franssen_3255
SEQUENCE=TTTAGGAAATACTCTCCTGGCCAGCTGTATCCCAAATCTGTAAGGTGAAAAGCCTATCTTCAAATTGCACTTCTTTGGTTAAGAAATCCGCTCCAATGGTTGCCTTGTACTGATTACTAAACTTCTTAATTCACATATTGGTTCATCAAAGACGTCTTCCCCACACCGCTGTCACCGAGAATGATGACCTTTAAGAGAGTTCTTCTGCGAGAAGGCATAGTACGGATCGGTGGAAGAAGAAGAAACTGTTTGCTTGTGTTTGATGATTCTTTTTTTACTTCAACTAGGACTCTCTCACACCGTCTTCAACTCTATTTCTCTCTCTCTAACTTTCTAAGCAGCCGYCTTTCTCTTTCTATCTACAAATTCTTGAGRACGG
TARGET=317,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00289 Ps_contig_mira-and-tgicl-ass_franssen_3277
SEQUENCE=GTCGGRKATTGAAGGTGAGTAMGTCGGGGAGAGATTCAAACATCTCTTCTTCTTTCCCTCACTCTACCCTCTCCTCTAAAAAAAATTCACTTTTTAATAGAATAATATTTTCAAGATTAATTTGTGCATCCTCTTTCAGTTTTCAAGAACATGTTTTTGATAGTTCCTTTCCAAATATGCACCTAGAGAGAGAGAGAAACAATAGCAAGGATCAATCTTCATTTGTGATATAGCTATTGAAGAAAGATTAGTAGAATTTTTGGCTTGCAATGATATGGCTTTCTTTCCATCAAATTTCATGCTTCAAACTTCTCATCAAGATGACCATCATCAACCTCCACCTTCACTCAACTCAATTATAACCTCATGCGCACCTCAAGATTACCATGGAGGAGTATCCTTTTTGGGAAAGAGATCTATGTCGTTTTCCGGGATAGAACTCGGAGAAGAAGCGAATGTGGAAGAAGAATTATCGGACGACGGATCGCAGTTAGGGGAGAAGAAGAGAAGGCTTAACATGGAACAAGTGAAGACCCTTGAGAAAAGCTTTGAGTTAGGAAACAAGCTTGAACCTGAGAGGAAAATGCAGCTTGCAAGGGCTCTTGGACTTCAACCAAGACAAATTGCTATATGGTTTCAAAACAGAAGGGCTAGATGGAAGACAAAACAGTTGGAGAAAGACTATGATGTTCTTAAAAGACAATATGATTCTGTTAAGGCTGATAATGATGCACTTCAAGCTCAGAACCAGAAGCTTCAGTCAGAGATATTGGCACTGAAAAATAGAGAACCAACAGAATCAATCAACCTAAACAAAGAAACAGAAGGATCAAGCAGCAACAGAAGTGAAAACAGTTCAGAAATCAAGTTGGATATTTCAACAAGGACGACACAAACAGCTATTGATAGTCCTTTATCCACTCAACAACAAACAAGCATAAACCTCTTTCCATCTTCTTCTAGACCAACACTAGTTCCTCATCAACTCTTTCAAACCAATTCGACAAGACAAGACATTAATCAGTGCCAAAAGATTGATCACATGGTTAAAGAAGAGAGTTTGAGTAACATGTTTTCTGCCATTGATGATCAATCTGGATTATGGCCATGGTTGGAGCAACAACACTTCAATTGATCAAATAAAAGAGTTTTCAGATTTAAAATTTTCTTGATTATCATACTCGCGCATGTTCGGATAAACAACGACTTTATGTCGTAGTGATTTTGACAAAAATACGCTATGAACTTTTAATAAAATCACAGTGTCGCTGTAATTTTGTCGAATTTACTGTCGATTTATTGAGTTGCGCAAGTTATAAACTTTGTTTTGGTGAACTTGATGTATAAAATAGGATATATATCAAGCATAATTTTTCAAGTTGAAGTTGAAATATATATATTCTTTCCAGTAATTAGTGAAATATTGCAGATTTGCAACTTTKTAAAAAA
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00290 Ps_contig_mira-and-tgicl-ass_franssen_3277
SEQUENCE=GTCGGRKATTGAAGGTGAGTAMGTCGGGGAGAGATTCAAACATCTCTTCTTCTTTCCCTCACTCTACCCTCTCCTCTAAAAAAAATTCACTTTTTAATAGAATAATATTTTCAAGATTAATTTGTGCATCCTCTTTCAGTTTTCAAGAACATGTTTTTGATAGTTCCTTTCCAAATATGCACCTAGAGAGAGAGAGAAACAATAGCAAGGATCAATCTTCATTTGTGATATAGCTATTGAAGAAAGATTAGTAGAATTTTTGGCTTGCAATGATATGGCTTTCTTTCCATCAAATTTCATGCTTCAAACTTCTCATCAAGATGACCATCATCAACCTCCACCTTCACTCAACTCAATTATAACCTCATGCGCACCTCAAGATTACCATGGAGGAGTATCCTTTTTGGGAAAGAGATCTATGTCGTTTTCCGGGATAGAACTCGGAGAAGAAGCGAATGTGGAAGAAGAATTATCGGACGACGGATCGCAGTTAGGGGAGAAGAAGAGAAGGCTTAACATGGAACAAGTGAAGACCCTTGAGAAAAGCTTTGAGTTAGGAAACAAGCTTGAACCTGAGAGGAAAATGCAGCTTGCAAGGGCTCTTGGACTTCAACCAAGACAAATTGCTATATGGTTTCAAAACAGAAGGGCTAGATGGAAGACAAAACAGTTGGAGAAAGACTATGATGTTCTTAAAAGACAATATGATTCTGTTAAGGCTGATAATGATGCACTTCAAGCTCAGAACCAGAAGCTTCAGTCAGAGATATTGGCACTGAAAAATAGAGAACCAACAGAATCAATCAACCTAAACAAAGAAACAGAAGGATCAAGCAGCAACAGAAGTGAAAACAGTTCAGAAATCAAGTTGGATATTTCAACAAGGACGACACAAACAGCTATTGATAGTCCTTTATCCACTCAACAACAAACAAGCATAAACCTCTTTCCATCTTCTTCTAGACCAACACTAGTTCCTCATCAACTCTTTCAAACCAATTCGACAAGACAAGACATTAATCAGTGCCAAAAGATTGATCACATGGTTAAAGAAGAGAGTTTGAGTAACATGTTTTCTGCCATTGATGATCAATCTGGATTATGGCCATGGTTGGAGCAACAACACTTCAATTGATCAAATAAAAGAGTTTTCAGATTTAAAATTTTCTTGATTATCATACTCGCGCATGTTCGGATAAACAACGACTTTATGTCGTAGTGATTTTGACAAAAATACGCTATGAACTTTTAATAAAATCACAGTGTCGCTGTAATTTTGTCGAATTTACTGTCGATTTATTGAGTTGCGCAAGTTATAAACTTTGTTTTGGTGAACTTGATGTATAAAATAGGATATATATCAAGCATAATTTTTCAAGTTGAAGTTGAAATATATATATTCTTTCCAGTAATTAGTGAAATATTGCAGATTTGCAACTTTKTAAAAAA
TARGET=1391,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00291 Ps_contig_mira-and-tgicl-ass_franssen_3284
SEQUENCE=CCTTAACGTCCAACCAACGTTCTAAACCAACCAAACCAATTTACCTAATACCCTAAAAATTTAACCTAATTCCAATACCTTTGTATNTTTAAAAAAAAGGTAACAAAATAACTAAGCAGACCCATGACACGAATTATTCAATAACACCCTAAAAACGAACGACTTGAGAAAAAAGTAACAAAATAGAAAATAATAGTAAAAGCCATGAATGAAAGGCTGCGATTTGATGATGATGATGATAACGATCACTTTTTGAGGCTTACCTACGTAACTACCTAGGGCGAGGCTTGGAAGATTCCGGAACAGCAGCTGCAGATGCGTGACGATTCACGAGCTTATCTTCCTCGAAAGGGGATTCGTTCGGGTGACGAGCATCGTGATGAATCTGCATCGTATTTCACATCGTAGGTTACCGTTTGTTTGCGTAGGT
TARGET=225,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0467
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.011696
PRIMER_LEFT_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_SEQUENCE=CGATGTGAAATACGATGCAGAT
PRIMER_LEFT=145,22
PRIMER_RIGHT=404,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.0467
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.011696
PRIMER_LEFT_1_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_1_SEQUENCE=AATACGATGCAGATTCATCACG
PRIMER_LEFT_1=145,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.1034
PRIMER_LEFT_2_PENALTY=0.035021
PRIMER_RIGHT_2_PENALTY=0.068420
PRIMER_LEFT_2_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_2_SEQUENCE=ATCCCCTTTCGAGGAAGATAAG
PRIMER_LEFT_2=145,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=59.932
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.1153
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=0.080323
PRIMER_LEFT_3_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_3_SEQUENCE=CAAACGGTAACCTACGATGTGA
PRIMER_LEFT_3=145,22
PRIMER_RIGHT_3=418,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.1576
PRIMER_LEFT_4_PENALTY=0.035021
PRIMER_RIGHT_4_PENALTY=0.122569
PRIMER_LEFT_4_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_4_SEQUENCE=CGAGGAAGATAAGCTCGTGAAT
PRIMER_LEFT_4=145,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=59.877
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_00292 Ps_contig_mira-and-tgicl-ass_franssen_3285
SEQUENCE=CTTCCAAGCCTCGCCCTGGTGTTCGTGGTAGTTCCTTCTAAAAAGTTGTATCGTTATCGTCATCATCATCAAATCGCAGCCTTTCATTCATGGCTTTTACTATATTTTCTATTTTTGTACTTTTTTTTCTTTCAAGTCGTCGTTTTTAGGGTGTTATTGAATAATTCGTGTCATGGGTCTGCTTAGTTATTTTGTCCTTTTTTTTTTAAATATCAAGATGATGTAATTTTAGGTATGAATGTTGTGTTGAACTGTTGCGATGTAAAGGTTTTGATGGATCGGNTT
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4827
PRIMER_LEFT_PENALTY=0.447703
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=CTGGTGTTCGTGGTAGTTCCTT
PRIMER_RIGHT_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT=15,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=60.448
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.4880
PRIMER_LEFT_1_PENALTY=0.453004
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GGTGTTCGTGGTAGTTCCTTCT
PRIMER_RIGHT_1_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=151,22
PRIMER_LEFT_1_TM=59.547
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.6132
PRIMER_LEFT_2_PENALTY=0.447703
PRIMER_RIGHT_2_PENALTY=0.165541
PRIMER_LEFT_2_SEQUENCE=CTGGTGTTCGTGGTAGTTCCTT
PRIMER_RIGHT_2_SEQUENCE=CCTTTACATCGCAACAGTTCAA
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=60.448
PRIMER_RIGHT_2_TM=60.166
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.6185
PRIMER_LEFT_3_PENALTY=0.453004
PRIMER_RIGHT_3_PENALTY=0.165541
PRIMER_LEFT_3_SEQUENCE=GGTGTTCGTGGTAGTTCCTTCT
PRIMER_RIGHT_3_SEQUENCE=CCTTTACATCGCAACAGTTCAA
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=59.547
PRIMER_RIGHT_3_TM=60.166
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.6648
PRIMER_LEFT_4_PENALTY=0.447703
PRIMER_RIGHT_4_PENALTY=0.217054
PRIMER_LEFT_4_SEQUENCE=CTGGTGTTCGTGGTAGTTCCTT
PRIMER_RIGHT_4_SEQUENCE=TACATCGCAACAGTTCAACACA
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=60.448
PRIMER_RIGHT_4_TM=60.217
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_00293 Ps_contig_mira-and-tgicl-ass_franssen_3286
SEQUENCE=GAACACCGTTTCATTCATCAACTCAATCCGATTCCCATCAAAACCTTTCATCGCAACAGTTCAAACACAACATTCATACCTAAAATTCATCATCTTGAATAATTTAAAAAAAAAGGACAAAATAACTAAGCAGACCCATGAACACGAATTATTCAATAACCAACCCTAAAAACGACGACTTAGAAAGAAAAAAAGTAACAAAATAAGAAAATATAAGTAAAAGCCATGAATGAAAGGCTGCGTATTTGATGATGATGATGATAACGATCTTCTTTTGGACGGCCTAACCCG
TARGET=247,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8970
PRIMER_LEFT_PENALTY=0.081185
PRIMER_RIGHT_PENALTY=0.815854
PRIMER_LEFT_SEQUENCE=ATCGCAACAGTTCAAACACAAC
PRIMER_RIGHT_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT=49,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.816
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=1.1630
PRIMER_LEFT_1_PENALTY=0.081185
PRIMER_RIGHT_1_PENALTY=1.081865
PRIMER_LEFT_1_SEQUENCE=ATCGCAACAGTTCAAACACAAC
PRIMER_RIGHT_1_SEQUENCE=TTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=286,21
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=1.3381
PRIMER_LEFT_2_PENALTY=0.522246
PRIMER_RIGHT_2_PENALTY=0.815854
PRIMER_LEFT_2_SEQUENCE=AAACCTTTCATCGCAACAGTTC
PRIMER_RIGHT_2_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=60.522
PRIMER_RIGHT_2_TM=60.816
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=1.4758
PRIMER_LEFT_3_PENALTY=0.659953
PRIMER_RIGHT_3_PENALTY=0.815854
PRIMER_LEFT_3_SEQUENCE=GAACACCGTTTCATTCATCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=59.340
PRIMER_RIGHT_3_TM=60.816
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=1.4876
PRIMER_LEFT_4_PENALTY=0.671790
PRIMER_RIGHT_4_PENALTY=0.815854
PRIMER_LEFT_4_SEQUENCE=ATAACCAACCCTAAAAACGACG
PRIMER_RIGHT_4_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_4=155,22
PRIMER_RIGHT_4=287,22
PRIMER_LEFT_4_TM=59.328
PRIMER_RIGHT_4_TM=60.816
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_00294 Ps_contig_mira-and-tgicl-ass_franssen_3287
SEQUENCE=CAAAAAGTGATCGTTATCATCATCATCAAATCGCAGCCTTTCATTCATGGCTTTTACTATATTTTCTATTTTGTACTTTTTTTCTTTCAAGTCGTCGTTTTTAGGGTGTTATTGAATAATTCGTGTCATGGGTCTRCTTAGTTATTYTGTCCTTTTTTTAAAATATCAAGATGATGTAATTTTAGGTATGAATGTTGTGTTTGAACTTGTTGCGATGTAAAGGTTTTGAGTGGGAATCGGATTGAGTTGTGCTTGTTAATTTAATTTAAGTTTCGGKCCCCTTTACTTAACGTCC
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00295 Ps_contig_mira-and-tgicl-ass_franssen_3311
SEQUENCE=ATAATACTATTAATTAATTAAATTTAATACGTTAAGCTTTCTCAGCCGCTACGTTCTTCACCACAGCCGTCACAGCCAGCGTGTTCTTTTGTTCTAATCCCATTCATTAATAATTTACCTGTTTAATTATCACTAATCCTCAATTACAACTCTTTATTCTCATTCTATTAATTAACTAATCTTTTCTCTAGAAAACGATTAATACCGATTTGTCACCTTTCGCCGTTTCTTCCTCTCTCTTTCATCATAACCTTTCCGACTCTGTTATCTTTCCCCGTTAACATTCTTTCTCTTCAATTTCACCTTTCTTATCTCTTCCTCTCAATTCCTCTCAATTCCTCTCAATTCCTCAACCACTCCTTCTCTTCAATCAGGTTTTTTTTCATCTGAAAGAACAGATCTACCTGAATCTATGGCGGAAGAACACCGATGCGAAACTCCGGAAGGTCACAGACTCTGCGCTAACAACTGCGGCTTCTTCGGGAGCTCAGCTACGATGAATTTCTGTTCAAAATGTTACCGAGACATTCATTTGAAGGAGCAAGAACAAGCCAAAACCAAATCCACAATCGAAACCGCTCTTTCTTCTGCCTCCTCCTCCGCCGCTACCGTAGTTGTTGAATCTGCGGTTCTAGAGATCGAATCTCTCCCTCAACCACCGGTACCGTCGATCGTGACTAACGCGTCGGAATCTTCGATCGGATCGGTTCAACCAAACCGGTGCGGGACGTGTAGAAAACGGACCGGGTTAACCGGATTCAAGTGCAGATGTGGAATCACGTTTTGTGGGACCCACAGGTATCCGGAGAAACACGAGTGCGGTTTCGATTTCAAGGCGGTGGGAAGAGAGGAGATAGCTAAAGCGAATCCTGTGATCAAAGCCGATAAGCTGAGAAGAATTTGATTTGATTTCCGCCGTTTGATGTGATTTCGATGATTTCATCTGGACCGTTCGCTGGGGAACTTACCTACACAAACGAATCATGTGGCGACGCTTGATGAAATGATGTAAAATAATCTATCTAATTAAAAAAGGTTGAATTAARTTTAAGTTTTTTTACCG
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00296 Ps_contig_mira-and-tgicl-ass_franssen_3312
SEQUENCE=GTTTCTTAAACAAAAGTCCTAAACAAGTAGAATGTAAAATAACATAACTTACAAAACCTAACTTCTTCCTAATTTTGCTACTAAACAAAAAACCAATAAAATAGTCTTTTTTTTTCCTAACAAAAGTAATTTACAAATAACTGTAATAACAGAACACCATCCACAAAATCCAAGAAGAATTTAACAAGTAAAAAAACAACTTTTCCATTCCCAAACTCACGTGTGTTTATGAAATCTAGATCCTCATACAAAAACCATAGTCGATAACCTCTCCCCGAGCGGAGCTCGCTCGCGGAGAGGATCCTAACGTCGGTTTTCGAAACAAGAACACACATCTAAAAAAGTTGTAGGGATATGGTTTGGGGAGAATGTAATGTGTCCATGATAAGAGAAGAAAAACAAAACATCCAAAACTTGTGAGGAATAAAATTCATGTTTCCAACCATACTCCAAACTTGGACTTGGGATCAGAAATCAAAGGATCATAACCCTTTATACATTCTTACGGATTAACATTGATAGGATCGGTTCGACCCTGCACGGCCATCCTCTCCGAGACATCCGCTAAAGAACTCAGGTTGCACCTGATCAACGCCTCTACGAAGTAACACGTTTCATCTCTCGTGTTCCCTTCAGGCACGTCCACGACGAACGATTCAATCACCATTGTACCTGGTCTTCCATCAATAACCTCCGGATGGACAGTGATTACGGAGGAATAGTTCCTGAGCCTATGATCRCCACCAACAATCCTGATACCGAGAATATGTTCTTCATCATCAAGCTGTTCCAACCTCTCAGTACTAGTTGTAGCCGGAAGACCAGATTTAACATTCACTTCTCTAACACTTCCAATGCCAAGATCACCTTGCATAATACACCTGCTAACAAATGGTTTATATTTCTGTGGCTGATCAAATCTTCTAACCAGAGACCAAACAAGATGAACAGGGGCTTTGATGTGTTTAACAAGTGCAGAGGAACACTGRTTGTCTCTTAAATCATGTTTGTGAYGTCTTCTTATGTACTGTGTCTCAATAGCAGAGTATTGTTCACCACCGTTGTTCATCTTGTTGGATGATTATTGTTTTGGTGGCTAGAACAAAAGAAAAAGAAGAAGAAGAATAAGAAGATAGAACAAGTGGTGGAGAGGGATAAGCTTGAGAGAGAGAGAGAGTAGWKRGWGTARARAGTGTGTAAGTAAATA
TARGET=1112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00297 Ps_contig_mira-and-tgicl-ass_franssen_3312
SEQUENCE=GTTTCTTAAACAAAAGTCCTAAACAAGTAGAATGTAAAATAACATAACTTACAAAACCTAACTTCTTCCTAATTTTGCTACTAAACAAAAAACCAATAAAATAGTCTTTTTTTTTCCTAACAAAAGTAATTTACAAATAACTGTAATAACAGAACACCATCCACAAAATCCAAGAAGAATTTAACAAGTAAAAAAACAACTTTTCCATTCCCAAACTCACGTGTGTTTATGAAATCTAGATCCTCATACAAAAACCATAGTCGATAACCTCTCCCCGAGCGGAGCTCGCTCGCGGAGAGGATCCTAACGTCGGTTTTCGAAACAAGAACACACATCTAAAAAAGTTGTAGGGATATGGTTTGGGGAGAATGTAATGTGTCCATGATAAGAGAAGAAAAACAAAACATCCAAAACTTGTGAGGAATAAAATTCATGTTTCCAACCATACTCCAAACTTGGACTTGGGATCAGAAATCAAAGGATCATAACCCTTTATACATTCTTACGGATTAACATTGATAGGATCGGTTCGACCCTGCACGGCCATCCTCTCCGAGACATCCGCTAAAGAACTCAGGTTGCACCTGATCAACGCCTCTACGAAGTAACACGTTTCATCTCTCGTGTTCCCTTCAGGCACGTCCACGACGAACGATTCAATCACCATTGTACCTGGTCTTCCATCAATAACCTCCGGATGGACAGTGATTACGGAGGAATAGTTCCTGAGCCTATGATCRCCACCAACAATCCTGATACCGAGAATATGTTCTTCATCATCAAGCTGTTCCAACCTCTCAGTACTAGTTGTAGCCGGAAGACCAGATTTAACATTCACTTCTCTAACACTTCCAATGCCAAGATCACCTTGCATAATACACCTGCTAACAAATGGTTTATATTTCTGTGGCTGATCAAATCTTCTAACCAGAGACCAAACAAGATGAACAGGGGCTTTGATGTGTTTAACAAGTGCAGAGGAACACTGRTTGTCTCTTAAATCATGTTTGTGAYGTCTTCTTATGTACTGTGTCTCAATAGCAGAGTATTGTTCACCACCGTTGTTCATCTTGTTGGATGATTATTGTTTTGGTGGCTAGAACAAAAGAAAAAGAAGAAGAAGAATAAGAAGATAGAACAAGTGGTGGAGAGGGATAAGCTTGAGAGAGAGAGAGAGTAGWKRGWGTARARAGTGTGTAAGTAAATA
TARGET=1163,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00298 Ps_contig_mira-and-tgicl-ass_franssen_3323
SEQUENCE=TAWGCATGATGATGATGATGATGAGAGAGAGAGAGAGGTTGAAGGTGGATGGCATGGTGTGGGGTCGGAGATGAAAGGTTGATGATGATTAAGACCGTGCGGGAAAGGGTTAATTAATGAATAATACTACTAGTAATAACGTGAGTAATGAAAAGTGTTGTAAATTTTTTTTGAAATTCATCGGTACAAGAGAATTACTGTTCTAATGGATTTTTTTTTCTTTA
TARGET=6,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00299 Ps_contig_mira-and-tgicl-ass_franssen_3323
SEQUENCE=TAWGCATGATGATGATGATGATGAGAGAGAGAGAGAGGTTGAAGGTGGATGGCATGGTGTGGGGTCGGAGATGAAAGGTTGATGATGATTAAGACCGTGCGGGAAAGGGTTAATTAATGAATAATACTACTAGTAATAACGTGAGTAATGAAAAGTGTTGTAAATTTTTTTTGAAATTCATCGGTACAAGAGAATTACTGTTCTAATGGATTTTTTTTTCTTTA
TARGET=23,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00300 Ps_contig_mira-and-tgicl-ass_franssen_3324
SEQUENCE=TTTTAMGWAAAAAAATACCATTAGAACAGTAATTCTCTTGTACCGATGAATTTACAAAAAAAATTTACAACACTTTTCATTACTCACGTTATTACTAGTAGTATTATTCAATTTAACTTTAACCCCTTTCCCGCACGGTTCTTAATCATCATCAACCTTTCATCCTCCCGACCCCACACCATACCATCCACCTTCACCTCTCTCTCTCTCNCATCATCATCATCATCATGTCTTAC
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00301 Ps_contig_mira-and-tgicl-ass_franssen_3324
SEQUENCE=TTTTAMGWAAAAAAATACCATTAGAACAGTAATTCTCTTGTACCGATGAATTTACAAAAAAAATTTACAACACTTTTCATTACTCACGTTATTACTAGTAGTATTATTCAATTTAACTTTAACCCCTTTCCCGCACGGTTCTTAATCATCATCAACCTTTCATCCTCCCGACCCCACACCATACCATCCACCTTCACCTCTCTCTCTCTCNCATCATCATCATCATCATGTCTTAC
TARGET=212,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00302 Ps_contig_mira-and-tgicl-ass_franssen_3325
SEQUENCE=AGTGGTATCAACGCAGAGTGGAATTAACTAGGGAGGAAGAAATGGGAATCGGGAGTTTGATTTGAGAGGTGCGAATAGAAAAAAAAATAAGACATTATGCGCAACGGTGTATAGATGAAAATGGATAGATGAAGAAACTGCATGATGATGATGATGATGAGAGAGAGAGAGGTAGGTTAGGAAGGTGGAGTGGCTAGTTGGGTGGGTGGGGGTACGGAARTAAGKTAAGGGTAAAAGGGTTTGAAGTAGAAGTTAGGTAAAACGTTTAAACCGGACCCGGGTAGGGGTAAAACGGGGGTTTAAAAAGGTTTTTAAAA
TARGET=142,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00303 Ps_contig_mira-and-tgicl-ass_franssen_3325
SEQUENCE=AGTGGTATCAACGCAGAGTGGAATTAACTAGGGAGGAAGAAATGGGAATCGGGAGTTTGATTTGAGAGGTGCGAATAGAAAAAAAAATAAGACATTATGCGCAACGGTGTATAGATGAAAATGGATAGATGAAGAAACTGCATGATGATGATGATGATGAGAGAGAGAGAGGTAGGTTAGGAAGGTGGAGTGGCTAGTTGGGTGGGTGGGGGTACGGAARTAAGKTAAGGGTAAAAGGGTTTGAAGTAGAAGTTAGGTAAAACGTTTAAACCGGACCCGGGTAGGGGTAAAACGGGGGTTTAAAAAGGTTTTTAAAA
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00304 Ps_contig_mira-and-tgicl-ass_franssen_3325
SEQUENCE=AGTGGTATCAACGCAGAGTGGAATTAACTAGGGAGGAAGAAATGGGAATCGGGAGTTTGATTTGAGAGGTGCGAATAGAAAAAAAAATAAGACATTATGCGCAACGGTGTATAGATGAAAATGGATAGATGAAGAAACTGCATGATGATGATGATGATGAGAGAGAGAGAGGTAGGTTAGGAAGGTGGAGTGGCTAGTTGGGTGGGTGGGGGTACGGAARTAAGKTAAGGGTAAAAGGGTTTGAAGTAGAAGTTAGGTAAAACGTTTAAACCGGACCCGGGTAGGGGTAAAACGGGGGTTTAAAAAGGTTTTTAAAA
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00305 Ps_contig_mira-and-tgicl-ass_franssen_3326
SEQUENCE=CCCCTAACCGGGTCCGTTTAACTTTACCGTAATCCAATCAAACCTTTACCCTACTTACTTCCGTACCCCCGAACCCAACCCAACCTAGTTCATCCACCTTCCTAACCTCTCTCTCTCTCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTTTTTTTCTTATTTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTACGGAAAAGAAATTACAAGTCTCATCGGCAACTAAAGAAAATTNTGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTAGAAACTAGAAAAACATTACTCATCGAAGACGGCGAAAGAAGATAGAAAACCGAAACCCCCCCCTAAAATTACG
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1563
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT=79,22
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.1563
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_1_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=180,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.5424
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.406341
PRIMER_LEFT_2_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_2_SEQUENCE=CATTATGCGCAACGGTGTATAG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.406
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=4.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.5429
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.406861
PRIMER_LEFT_3_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_3_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.593
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.5429
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.406861
PRIMER_LEFT_4_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_4_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.593
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_00306 Ps_contig_mira-and-tgicl-ass_franssen_3326
SEQUENCE=CCCCTAACCGGGTCCGTTTAACTTTACCGTAATCCAATCAAACCTTTACCCTACTTACTTCCGTACCCCCGAACCCAACCCAACCTAGTTCATCCACCTTCCTAACCTCTCTCTCTCTCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTTTTTTTCTTATTTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTACGGAAAAGAAATTACAAGTCTCATCGGCAACTAAAGAAAATTNTGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTAGAAACTAGAAAAACATTACTCATCGAAGACGGCGAAAGAAGATAGAAAACCGAAACCCCCCCCTAAAATTACG
TARGET=118,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1563
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT=79,22
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.1563
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_1_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=180,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.5424
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.406341
PRIMER_LEFT_2_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_2_SEQUENCE=CATTATGCGCAACGGTGTATAG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.406
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=4.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.5429
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.406861
PRIMER_LEFT_3_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_3_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.593
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.5429
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.406861
PRIMER_LEFT_4_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_4_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.593
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_00307 Ps_contig_mira-and-tgicl-ass_franssen_3330
SEQUENCE=TTACKGAGTTAGTTATCATCGGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCACTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACCAAGATGAGA
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00308 Ps_contig_mira-and-tgicl-ass_franssen_3330
SEQUENCE=TTACKGAGTTAGTTATCATCGGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCACTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACCAAGATGAGA
TARGET=203,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00309 Ps_contig_mira-and-tgicl-ass_franssen_3330
SEQUENCE=TTACKGAGTTAGTTATCATCGGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCACTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACCAAGATGAGA
TARGET=287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00310 Ps_contig_mira-and-tgicl-ass_franssen_3331
SEQUENCE=CGAACATCTTGTWTGCAAGAATAAGAACTGTTACTAACATAGAAAAATCAGCATAACTAGCATCAAATAACAAGCTAAAGAAGAATCACAACTAAATCAAACCCAGCRTAAAAAACACTATTATCATTGCCGTTTTCAGGATACAACATAGATAATCCCCTCAAATGTTTGAACCAAATTACACTGGATATCAGATTTAAGTTATTTGAAAGTTTAACCAAAACTAACATTTACCGAAGAACACAAACCCTAATCAATCAATCGTGCAGAATACCCAAAATGTCCTCGTCCCTAAACAAATGGAACTCTTTGTCATCAAGCTTAATTTGAGAGCCACCGTACTCCGGCAAGAGAACGTGGTCACCTTC
TARGET=252,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00311 Ps_contig_mira-and-tgicl-ass_franssen_3355
SEQUENCE=AAGCAGGTGGTATACACGCAGAGTACGSGGACACCATCACCACATTCGTCTTCTCTTTCTCTCTCTCTCAATTCCTCCTTTTCAGGAATTTGGAGAGAGTAGTGATTAACCAAACATGAAGTTTTCAATGTATTTTTCTTGTTAGCAACATTCTATTGCTTCTAAGTTCTTCACCAGCTATTTYGACCAAAACTGTAACGTCCACCGGCACCGATCGCGCCGACACCGCTCCAACTCCCGGCCCCAGCCACCTGCCAACCAGTA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00312 Ps_contig_mira-and-tgicl-ass_franssen_3357
SEQUENCE=TTACCAACGGACGTGTGGCGGCCCGACTGCCTCTCAAAAACTTAACTTCTATTACTTGAAAATAGCTTTCTTCCTTAATTGTCATTGTTCTTTAGTCAAAATCACGTGTCCCCTTAGGAATCTTGGGCAATCTTCTGCTTTCATTGCTTTCTACAACATCCTTTACTTTCAATAAAAATTCATTGAATTCAGGTCTTGCCAAATCCAAAAACAATAGAAAATCAGCAACCCAAGTCTCTACATCACTCTTTTTTTCACTCTGCAACCTATCCTTAATCAACTGCAAAATCGAACTAGTTCCTTTTCCCAAAACAGCCTTCTTCTTCTTCTTTTCTACTTTGGAATTCTCTCCATTTTCTGTAGCACCTCGGATCTTTTCATTCAACTCACCAGCCTCAAGTACAGCATAAGCAGTCACAAGTTCCCACGCCACAATTTTCCACACAAGCGCAAACAACACATTGATTTCATGTGCAAACTTATCATACACCTTTCCAGAATACAAATTGAAAGACTCGTTAAAACTACTAAGCAACCTAGCATCAGTTGGACATTCCTTCCCAAAGATTTCACCAATTTTCGATTTCAAATCATCACCACCAATAGCCAACAAGTTACTTTGTGCACGAGCAAAACTACAGTCACCCAATTCCCTCAATGCCATTGCCAAAGGCAACAACACATTGCACACAGTATGCTCACTCCCAAGCTCAATTTTCCCCAATTTATGACCGGAATTCAACTCAACTCGCATCCTAGAATGCACTGATTTCGCCTTCTCCCTAATAATCCCATGAATTTTAGGAATTTTCACAACCGAAGAAACAGTTTCCTTTCCCACAAACTTAGATCCATTAAGCAAAATCCTCATATCACCAGCAACTCCAACTTCCTCCTTAGAACTATGTCCATCACCGGAATCCATCAAATTGAAAACACTCACATCAGCCTTCAAAGCCTCACAAGAAAACGCCGCAGCCACATCAGCAAAAGCAGACAGCGCCGTCGATTGATGATCCAAAACTGCGGAAACACCAATCAACCCTAAACACCCATTATTGATCAACAATGACTGTTCGTCTTCAGTCACATCGAGTTCTTCTTCGAAATGAAAACTCTTCTGATTCAAATTCAGTGTTTCTGATATGCATAATGGAACGTGTTTAGATGAGTTGCAGAGGAGGAGTTTGTTGAGAAGGACGATGGAGGAAGGCTCTTGTTTCTTCGTAGGGTTAGGTAA
TARGET=317,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2120
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_SEQUENCE=AATGAAAAGATCCGAGGTGCTA
PRIMER_LEFT=216,22
PRIMER_RIGHT=381,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.3786
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.261998
PRIMER_LEFT_1_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_1_SEQUENCE=AGATCCGAGGTGCTACAGAAAA
PRIMER_LEFT_1=216,22
PRIMER_RIGHT_1=374,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=60.262
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.3786
PRIMER_LEFT_2_PENALTY=0.116582
PRIMER_RIGHT_2_PENALTY=0.261998
PRIMER_LEFT_2_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_2_SEQUENCE=AAGATCCGAGGTGCTACAGAAA
PRIMER_LEFT_2=216,22
PRIMER_RIGHT_2=375,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.3786
PRIMER_LEFT_3_PENALTY=0.116582
PRIMER_RIGHT_3_PENALTY=0.261998
PRIMER_LEFT_3_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_3_SEQUENCE=AAAGATCCGAGGTGCTACAGAA
PRIMER_LEFT_3=216,22
PRIMER_RIGHT_3=376,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.3786
PRIMER_LEFT_4_PENALTY=0.116582
PRIMER_RIGHT_4_PENALTY=0.261998
PRIMER_LEFT_4_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_4_SEQUENCE=AAAAGATCCGAGGTGCTACAGA
PRIMER_LEFT_4=216,22
PRIMER_RIGHT_4=377,22
PRIMER_LEFT_4_TM=60.117
PRIMER_RIGHT_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_00313 Ps_contig_mira-and-tgicl-ass_franssen_3358
SEQUENCE=TTCGGGTATACCAGATAATCTCCGTTGGAGGGCATTACAGTAAATGCAAGAATTATTGCCGCCGCCTTCGTCATTACCATCCCGGTGGGCCCGTTTCGCGATCTTCAAATTTTTGGAAGTTCCTTATAAGAAAACGCTTCCAAATAAKKSRSGACCCTTGATTTTGAATTCACGATAGATAAACAAGATAAGGTGTTTTTGTTTTTGCGTGTGTGTGTATGCGAAGGAAGAAAGAGTTTTTCGAGCAATGGGAAGAGCGTTCGTGTACGTGATTCTCGGTGGTGGTGTTTGAGGCATTTTATAAGAAAGATCTTGCTAAAAGACTACTGTTAGGAAAGAGTGCTTCGATTGATGCAGAGAAATCCATGATCTCCAAGTTGAAGACTGAGTGT
TARGET=209,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00314 Ps_contig_mira-and-tgicl-ass_franssen_3361
SEQUENCE=AGTACGCGGGACCGTCCCTTTCCCCTTTTCSAATGATCTCAATTCATAACTCTTCTTTCTTTCCCCACCTATGCATTCATCACATTTTCCCCAAATTTCTCAATCTCTTCCTCTTCCCAACCCTAAATTCTCCATCCTTTCAAAACCCTAATCCAAACCCAAACCCTCTTAAGGGCTTTTAAACCTTTTCCTTAAATTCATCATTTACTCACTCAGATAACAATTCAAACCAAAAGCTCAAACCTTTCCCATCCCAGATTGCTTTTTTCATCCACTTCATTTTCAATATGCACGCAAAAACTGATTCTGATGTTGCAAGTTTCGACCCATCGTCACCCAGTTCACCAAAACGAACCGTTTACTATGTTCACAGTCCTTCTAGAGACTCTCATGATGGTGACAAATCTTCAACTATGCAACCAAGTCCTATGGATTCACCTTCACATCAATCCTATGTTCACCATTCAAGAGCTTCTTCTTCCAGCAGAATCTCTGGTGGTTATAACTCTTCTTTGGGTAAGAAAGGGAATAGAAAAGGTAATGATAAAGGCTGGCCTCATTCTAAGGTTATAGAAGAAGAAGATGGTGATTTTTATCATGAGAGTAATGGAATTTCTAGGAGGTTTCAAATCTTCATTGCTATTGTCGGGTTTGTGCTGGTTTTGCYGTCTTTTG
TARGET=572,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00315 Ps_contig_mira-and-tgicl-ass_franssen_3363
SEQUENCE=TAACTTTCTCATTAGGTCATGCTGATGACAAATGCAAAGAGCCTCTTGCTGTTATGGCCTTTGCTGGTGACCAAACTCAGAACATTCATTAAACAAACAAACCAAAATTACGAATTCTACTTTTCAAGTTTCTAATCTGAATTTCACTGCCAAAGCTGATATGACTAGAATTGCCTTCTATAGTGTTTACTACAATACTAGAAGTGATGATATGAGCTCTCTTTGTGGGCCTGTTGTGGATGATGTGGAGGGTTTGGTTTTCTAGGTCTAACGGGCTTCGTGGGCTGGGCTTTGAAGACTTGGGCTTGGGTATTTTKGGKTTTAGTTTTGGTTCTTTAGGTTTWAAARGGGGTTNATTTATTGGTTTTTGGTTTATTTAATGTTTATTACTACTAAGCTTGATTAGGTTTCTTTTGTAGTTTTGGGGG
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00316 Ps_contig_mira-and-tgicl-ass_franssen_3404
SEQUENCE=TAGCTTAGGTTTTTTCCTCTGTTGGTAAACCAGCTCCATYAGCACTCTCACACTCGATCTCAYACAAAGTTCCAAAAATCAAAATCAGTCTCATCCACTTCCAATTTCAACCCCTTTCCAATCATWAAMATTCCTCACATTCCGAAAACCTACCTAAACCTAAAAACCCAATTTTCTACCCACCCACCCCAAACTTCCTCCTTCACCCTTTTCTAGAATCCTCGACTCCAACAAACCCACGCTTCCCCGGTTCCGAGAACACATTCAC
TARGET=178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00317 Ps_contig_mira-and-tgicl-ass_franssen_3418
SEQUENCE=AAGGCAAAGACACGCTCGTCTTTCACTGTTCGTCTTTCTTTAAGTTTCTTAGGTTTTCGTTAGGTTCCCTTAAGTTTTGTTGTCAAGGAAGCTTGTCAATTATCTTGAAGAGAACAAGGAAGATGTTGGGATAAAGTTAACTATTATGGTGTTGGAACGTGGCTTCAATGGGTGGGAAGCTTCTGGTAGACCTGTTTGCCGCTGCAACAATGTTCCTTGTAAGGAGCATGAGAATGCTTGATCAAAGGCTTATTCTGGTANACCCATCTTACCTGTACTATCTGCAAACTNTTTGATAACTAAAGAAATGTATGAACTTATTAATTAATTACATCATTATAATAATCATAGGTAAGCTAG
TARGET=320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00318 Ps_contig_mira-and-tgicl-ass_franssen_3481
SEQUENCE=AAAAGTTTCAACATCCAAGAGATATAATTCAGACCAACCAGTTACATTATGTAATCTGCAGCCCATAAACTTGTTGGAGCTTCGCATAAAGACTCGAAGTGCGTATAGTATACACAGAAACACAGAAAACAAGTTTAACTAAGATGGAACAAACAGAATAAATAATTATGAACAACATGATGCAGCTCTGGATCCTTCGGCGGGTCTATCAACAAGAACCATTCCTGCTGGTTGTGCTGGCTGTGCCCTCGGTAACCTCTTGGCTATTTCATAAAATATTTCATTGACATTGGCTGCAGTTTTTGCAGAGGCCTCAATGAAAAACAGACCATTTTCCTCAGCATATACACGTGCTTCTTCAGCTGTCACTTTCCTTTTATCTTCCAAGTCAGCTTTGTTACCGGCAAAAGCCACGACCATGTTAGGATTCCCTTGTTTCTGAAGCTCTTGGACCCATTTCTTCGCCCGTGTAAATGAATCCAAGTTAGTAATGTCATAGACAATGATAGCAGCAGCTGCGCCTCTATAGTACATGGGGGCCAAGCTATGGTATCTCTCTTGTCCTGCTGTGTCCCAAATCTCAAATTTCACTGTGGCGTCATTTACTGCCAGCGTCTGGGAGAAAAATGCTGCCCCTATTGTTGATTCCTGAAACTCAAGGAATTGACCCTTGACAAAACGCAATACAAGGCTTGATTTTCCAGCACCCATATCCCCAAGCAGAACCAATTTGGCGTTGAGGTTGCTGTGTCCGATCGTAGCCATTGAAGTGAAATTGGAGCGATCGGAATGGAAGAAGCTTCGAGACGAAGAAATTGAAATTAAAAAAAAAGAGGAAGAAGAAGAAGACGCCGAAAACAGAGTCCTTCTCCTAGTTGTATTGTTATAGCTTTTAGCTTTGCGTGCAAACGCGCTTTTCTTTTCTTTTACTACTATTTTCTAATGTTTTTTGTTCWSMGACAGATTCCACTCAGCTTTCC
TARGET=836,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00319 Ps_contig_mira-and-tgicl-ass_franssen_3481
SEQUENCE=AAAAGTTTCAACATCCAAGAGATATAATTCAGACCAACCAGTTACATTATGTAATCTGCAGCCCATAAACTTGTTGGAGCTTCGCATAAAGACTCGAAGTGCGTATAGTATACACAGAAACACAGAAAACAAGTTTAACTAAGATGGAACAAACAGAATAAATAATTATGAACAACATGATGCAGCTCTGGATCCTTCGGCGGGTCTATCAACAAGAACCATTCCTGCTGGTTGTGCTGGCTGTGCCCTCGGTAACCTCTTGGCTATTTCATAAAATATTTCATTGACATTGGCTGCAGTTTTTGCAGAGGCCTCAATGAAAAACAGACCATTTTCCTCAGCATATACACGTGCTTCTTCAGCTGTCACTTTCCTTTTATCTTCCAAGTCAGCTTTGTTACCGGCAAAAGCCACGACCATGTTAGGATTCCCTTGTTTCTGAAGCTCTTGGACCCATTTCTTCGCCCGTGTAAATGAATCCAAGTTAGTAATGTCATAGACAATGATAGCAGCAGCTGCGCCTCTATAGTACATGGGGGCCAAGCTATGGTATCTCTCTTGTCCTGCTGTGTCCCAAATCTCAAATTTCACTGTGGCGTCATTTACTGCCAGCGTCTGGGAGAAAAATGCTGCCCCTATTGTTGATTCCTGAAACTCAAGGAATTGACCCTTGACAAAACGCAATACAAGGCTTGATTTTCCAGCACCCATATCCCCAAGCAGAACCAATTTGGCGTTGAGGTTGCTGTGTCCGATCGTAGCCATTGAAGTGAAATTGGAGCGATCGGAATGGAAGAAGCTTCGAGACGAAGAAATTGAAATTAAAAAAAAAGAGGAAGAAGAAGAAGACGCCGAAAACAGAGTCCTTCTCCTAGTTGTATTGTTATAGCTTTTAGCTTTGCGTGCAAACGCGCTTTTCTTTTCTTTTACTACTATTTTCTAATGTTTTTTGTTCWSMGACAGATTCCACTCAGCTTTCC
TARGET=914,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00320 Ps_contig_mira-and-tgicl-ass_franssen_3532
SEQUENCE=TAAAGGTTAACATCCTAATGTTGCTGAGATGAGAAGGATATATATAATGTAATGTAATGATTAAACTCTCTAAACTTTTGTATTAACTTTTTTATTCTGTCGGTTTTGTTTTGAGCACTTAACAAGAGAAGTGTCTTTTGTGACTTCTTATTTTCTATCATCACTATATTCATGTACTAAAATTTAAAAGT
TARGET=45,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6163
PRIMER_LEFT_PENALTY=1.063505
PRIMER_RIGHT_PENALTY=0.552823
PRIMER_LEFT_SEQUENCE=AATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_SEQUENCE=AAGTGCTCAAAACAAAACCGAC
PRIMER_LEFT=16,22
PRIMER_RIGHT=119,22
PRIMER_LEFT_TM=58.936
PRIMER_RIGHT_TM=60.553
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=2.3405
PRIMER_LEFT_1_PENALTY=1.063505
PRIMER_RIGHT_1_PENALTY=1.277028
PRIMER_LEFT_1_SEQUENCE=AATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_1_SEQUENCE=GCTCAAAACAAAACCGACAGA
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=115,21
PRIMER_LEFT_1_TM=58.936
PRIMER_RIGHT_1_TM=60.277
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=2.5766
PRIMER_LEFT_2_PENALTY=1.063505
PRIMER_RIGHT_2_PENALTY=1.513104
PRIMER_LEFT_2_SEQUENCE=AATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_2_SEQUENCE=GCTCAAAACAAAACCGACAGAA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=115,22
PRIMER_LEFT_2_TM=58.936
PRIMER_RIGHT_2_TM=61.513
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=2.6399
PRIMER_LEFT_3_PENALTY=2.087031
PRIMER_RIGHT_3_PENALTY=0.552823
PRIMER_LEFT_3_SEQUENCE=CTAATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_3_SEQUENCE=AAGTGCTCAAAACAAAACCGAC
PRIMER_LEFT_3=14,24
PRIMER_RIGHT_3=119,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=60.553
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=2.6399
PRIMER_LEFT_4_PENALTY=2.087031
PRIMER_RIGHT_4_PENALTY=0.552823
PRIMER_LEFT_4_SEQUENCE=TCCTAATGTTGCTGAGATGAGAAG
PRIMER_RIGHT_4_SEQUENCE=AAGTGCTCAAAACAAAACCGAC
PRIMER_LEFT_4=12,24
PRIMER_RIGHT_4=119,22
PRIMER_LEFT_4_TM=59.913
PRIMER_RIGHT_4_TM=60.553
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_00321 Ps_contig_mira-and-tgicl-ass_franssen_3535
SEQUENCE=TTTCACGRCCCAAAGTCACTGTCTTGATTGACATTAAGAAACACCAAACAAACCATCTCAATAAACTAAGCTACCACTAMTACTAATATTAAATAAATAAA
TARGET=90,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_00322 Ps_contig_mira-and-tgicl-ass_franssen_3554
SEQUENCE=TAAAATTAACTTAGTCATCATAAAAATTACTAGYCTTAAAATTACTTGCATCAAGTACAACCAAAATTACTGCNTTAAAAGGACATGTAGAAATTACTGCATAAAATGCATCAAAATTCTGCTGCCAAGCCAAACAAGCCACAAGCGAGCCAAGCCGGTAAACCGCAAGCAAAACGCAGCTAATACACAAACCTTCTAACAAAACCTGCATAAAAGAAAGCAAAAGCATAACAGTGGAAAGGAGGCAAAATAGAAGCATAGCATAATAGAGAATTGGAAACACTCACTGAAACCATGACTAACTTCGTGTGAGAACCGACTCGGTAACTAACCTGCCAAGTGCCATAACCGCCTCATAACAGAATAACTAACTCCACAAGATAGAAATCCAACTTCAAAACACCTTCAGAACCTATTTTCCTCATCCTCCTTCAACTCTCATAACAACTTCCAACTGTCATAACCAACCATCTCTCTAACCTTACTCTCCTATAAGAACCATCCTCCATCTTCAATTCAAGTTTCTTCCATCATCTTTCCCATAATTCAACAATTCACCATTCTTCTCCACCTCCACTCTCAATCCACTTCCAGCTTCTTCGTCAAAACCTTCACCAAACCCCAAATTCCATAAACATAATCTTCTTCAACATCAACCTTCACCTTCCAATCCACACCTTCACCACTACGGATCCGTAACCGTAATCTCCACCTTCACAAGATCTTCAACAACCTCTCTACAATTTTTCCGGGATTCTCCTTTTCTTCCTCCATCGCCTATTCAATTCTTCAACCTCATCACAAACTCTTCGAATCATTGAACCCAAACCTTCAACCACCGTGAATCCATCCAAGAACTCCTCTTCATCATAACTGCAACCAACCAAAACCGTAACCGAAACCGTAATCTCCACCTTCATCAAACCTTCACCAATTCGCAACGGTGCGGTCACGACGAAATCGCGGTCAAAAATCAAACGCCTTCATCTCCGTTACGGTCAACGACGCGCCACGACGAACAACAACAACACGGCTCGAACACGCGGGTTCGTGTGATTCTTCTGGTGACGAAGGAGGATACGAAGAAAGAGAAGTAGAAAGTGGAACACATCGCTTGATTACGGATCTGATCAAGGTGGACGTCAGCTTCACTCCGACGACGTGTTGGTAACGACATTACGCAAACCGTTCCACTCTGGCGAAGACGCATCGACGGCGATGATGCACGGTTGAGGCGCGAGCAGTTTTACTCCGCCGGTAGCGTGTTGACGACGATGATGTGCGGTTGTTACACCAGCAGTTTCCGTTCATGGACGTTTTGACTCCTGAAGGTGAGTTTCGGTTTTCTCATCTGAACCTGTCTTTAGGGATTTCATAGAACATTTTCAATTACCCCGT
TARGET=1018,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00323 Ps_contig_mira-and-tgicl-ass_franssen_3587
SEQUENCE=CCCTTTCAAGTGTTGAATTCCAACCACAAATGTGTTAAGAAATCCTCCAATTGAAGRATGTGATAATATCTTACCACTTGTGGAGCCCAACCTCTAATTATAAGGCCTCTCTCTTTGTTCCTTTCCTCAACCTTTCTTCACTAATCCATTTTTCCAACTCTTCACTTTTATTTCCTTCTCTAATAACCCAAATAAATGGCCTTTGTGTTGCTTCCAAGGCTAAAGCCAATTCCATAAGCTGTGAAGGAACTAAGTTGCATAAACTTCCAAGACAAGCATAGATCACAGACTTTGGTTGGTGCAAATCTAGCCAATTTAAGCAATGATGTTGACTTATTGAAGCCATGTTACCTCTTTGTGCTTTGTCCAACCCATCTTTGTTGCAAAGGGAAACAGGTCCAACACACCACACTTTATCATTTCTTTCTTTCTTGTAATCCTTAACATATGCTTCCTCCAACTCCTCAAAAGTGTTTATGATTTCACCATATGACCTTACTTCAGCCTCATTCATCTTCTCTTTAAACTCCTTAAACTCATCTGGTATTGTTCCTCCTGGTACTTGCACTTTGGTGACATGAATTTGGTCAGGAATACCAGGCACAGTGAAATACTCAGATTCTGAATCGATACTTTCAAGAATCTTCGATGCATTTACCTTGACCCGCGTACTCTGCGTTGA
TARGET=420,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00324 Ps_contig_mira-and-tgicl-ass_franssen_3594
SEQUENCE=GATTCAGACTTTGATTTTTTTCCCGAAAATAATAAAATAAAATAAAAAATAGCCCTGTGTTTTTCCATTTCCTCTAAGAGAGTGAGTTAATGGCAGAAGAAGAAGAAGAATCTGAACTCAGCGAGAAGCAAAAAGTTGAAATCGCGAAATGGTTTCTTCTCAACTCTCCTCCCGGAGAGATACGAAATCCGAAAGAGATAAAGAACATGCTTGACTTTCATCCCCAACGGATTGGACATGCTTGTTTCTTCGG
TARGET=31,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00325 Ps_contig_mira-and-tgicl-ass_franssen_3594
SEQUENCE=GATTCAGACTTTGATTTTTTTCCCGAAAATAATAAAATAAAATAAAAAATAGCCCTGTGTTTTTCCATTTCCTCTAAGAGAGTGAGTTAATGGCAGAAGAAGAAGAAGAATCTGAACTCAGCGAGAAGCAAAAAGTTGAAATCGCGAAATGGTTTCTTCTCAACTCTCCTCCCGGAGAGATACGAAATCCGAAAGAGATAAAGAACATGCTTGACTTTCATCCCCAACGGATTGGACATGCTTGTTTCTTCGG
TARGET=95,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5760
PRIMER_LEFT_PENALTY=0.343977
PRIMER_RIGHT_PENALTY=0.232060
PRIMER_LEFT_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_RIGHT_SEQUENCE=AGTTGAGAAGAAACCATTTCGC
PRIMER_LEFT=49,22
PRIMER_RIGHT=164,22
PRIMER_LEFT_TM=60.344
PRIMER_RIGHT_TM=59.768
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.7043
PRIMER_LEFT_1_PENALTY=0.343977
PRIMER_RIGHT_1_PENALTY=0.360320
PRIMER_LEFT_1_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_RIGHT_1_SEQUENCE=AAGAAACAAGCATGTCCAATCC
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.344
PRIMER_RIGHT_1_TM=60.360
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.7326
PRIMER_LEFT_2_PENALTY=0.500551
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=CCTGTGTTTTTCCATTTCCTCT
PRIMER_RIGHT_2_SEQUENCE=AGTTGAGAAGAAACCATTTCGC
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=0.8609
PRIMER_LEFT_3_PENALTY=0.500551
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=CCTGTGTTTTTCCATTTCCTCT
PRIMER_RIGHT_3_SEQUENCE=AAGAAACAAGCATGTCCAATCC
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=1.1206
PRIMER_LEFT_4_PENALTY=0.343977
PRIMER_RIGHT_4_PENALTY=0.776671
PRIMER_LEFT_4_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_RIGHT_4_SEQUENCE=CTTTCGGATTTCGTATCTCTCC
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.344
PRIMER_RIGHT_4_TM=59.223
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_00326 Ps_contig_mira-and-tgicl-ass_franssen_3595
SEQUENCE=GAAGTACGCCGGGGGGGAGGGTAAACCGWAACCTTTWCTAAGTTCAATCTGTTTTACTATTTGTAAGCTGAAAGAGAAAGTTCGTGTTGTGCATAGTTTCAAGAAAGTTAAAACTGATGATGATGAAGCATGGCAAGTGTTGAGAGGAGAGGTTTTTAGCTGCAGTGAGAATTTGGAGTTGATTAAGGTTGGATTTGAAGAGATACTGGAGAAAGACATGGATTGTAACAAGGTTCATCAAAATAGTGTTCTTGAGTATCTTGAAAGGGTTGATGAATGTAATAGTCTTAGGTTTAAAAGGGGTAAATACGAAGGGTTTTGGGATAGGGAGTAAGTAATTGGTTGGAAGGTGGGGGAAAGAACCGGCGGATTAATTAAAATCGGGCCAA
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00327 Ps_contig_mira-and-tgicl-ass_franssen_3617
SEQUENCE=ATCAAGATCTTCAATACCGAGTAATCTTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCANGTCCCGGTTTTTTTTTAAGAACAAAGACTAACCAAAAACCCTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGGTAGGTACAAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTAAGGAAGGNGTGAGAG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6612
PRIMER_LEFT_PENALTY=0.502921
PRIMER_RIGHT_PENALTY=0.158317
PRIMER_LEFT_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT=66,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.497
PRIMER_RIGHT_TM=59.842
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.6847
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=0.340219
PRIMER_LEFT_1_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_1_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=338,22
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=59.660
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.7491
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.404566
PRIMER_LEFT_2_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_2_SEQUENCE=ACCATACTTTCTTCCGTCGTTG
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=302,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=60.405
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.7491
PRIMER_LEFT_3_PENALTY=0.344527
PRIMER_RIGHT_3_PENALTY=0.404566
PRIMER_LEFT_3_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_3_SEQUENCE=CAACCATACTTTCTTCCGTCGT
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.7810
PRIMER_LEFT_4_PENALTY=0.502921
PRIMER_RIGHT_4_PENALTY=0.278124
PRIMER_LEFT_4_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_4_SEQUENCE=CACAGCAACTGAATGATGATGA
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.497
PRIMER_RIGHT_4_TM=59.722
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00328 Ps_contig_mira-and-tgicl-ass_franssen_3617
SEQUENCE=ATCAAGATCTTCAATACCGAGTAATCTTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCANGTCCCGGTTTTTTTTTAAGAACAAAGACTAACCAAAAACCCTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGGTAGGTACAAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTAAGGAAGGNGTGAGAG
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6612
PRIMER_LEFT_PENALTY=0.502921
PRIMER_RIGHT_PENALTY=0.158317
PRIMER_LEFT_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT=66,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.497
PRIMER_RIGHT_TM=59.842
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.6847
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=0.340219
PRIMER_LEFT_1_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_1_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=338,22
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=59.660
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.7491
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.404566
PRIMER_LEFT_2_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_2_SEQUENCE=ACCATACTTTCTTCCGTCGTTG
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=302,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=60.405
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.7491
PRIMER_LEFT_3_PENALTY=0.344527
PRIMER_RIGHT_3_PENALTY=0.404566
PRIMER_LEFT_3_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_3_SEQUENCE=CAACCATACTTTCTTCCGTCGT
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.7810
PRIMER_LEFT_4_PENALTY=0.502921
PRIMER_RIGHT_4_PENALTY=0.278124
PRIMER_LEFT_4_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_4_SEQUENCE=CACAGCAACTGAATGATGATGA
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.497
PRIMER_RIGHT_4_TM=59.722
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00329 Ps_contig_mira-and-tgicl-ass_franssen_3617
SEQUENCE=ATCAAGATCTTCAATACCGAGTAATCTTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCANGTCCCGGTTTTTTTTTAAGAACAAAGACTAACCAAAAACCCTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGGTAGGTACAAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTAAGGAAGGNGTGAGAG
TARGET=207,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6252
PRIMER_LEFT_PENALTY=0.284978
PRIMER_RIGHT_PENALTY=0.340219
PRIMER_LEFT_SEQUENCE=TTCATCATCATCATCATCCTCC
PRIMER_RIGHT_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT=180,22
PRIMER_RIGHT=338,22
PRIMER_LEFT_TM=59.715
PRIMER_RIGHT_TM=59.660
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.6612
PRIMER_LEFT_1_PENALTY=0.502921
PRIMER_RIGHT_1_PENALTY=0.158317
PRIMER_LEFT_1_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_1_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=59.497
PRIMER_RIGHT_1_TM=59.842
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.6847
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.340219
PRIMER_LEFT_2_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_2_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=338,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=59.660
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.6895
PRIMER_LEFT_3_PENALTY=0.284978
PRIMER_RIGHT_3_PENALTY=0.404566
PRIMER_LEFT_3_SEQUENCE=TTCATCATCATCATCATCCTCC
PRIMER_RIGHT_3_SEQUENCE=ACCATACTTTCTTCCGTCGTTG
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=59.715
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.6895
PRIMER_LEFT_4_PENALTY=0.284978
PRIMER_RIGHT_4_PENALTY=0.404566
PRIMER_LEFT_4_SEQUENCE=TTCATCATCATCATCATCCTCC
PRIMER_RIGHT_4_SEQUENCE=CAACCATACTTTCTTCCGTCGT
PRIMER_LEFT_4=180,22
PRIMER_RIGHT_4=304,22
PRIMER_LEFT_4_TM=59.715
PRIMER_RIGHT_4_TM=60.405
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_00330 Ps_contig_mira-and-tgicl-ass_franssen_3619
SEQUENCE=GTGCGGGGACTTCTTCTCAAACTCTCTCCTTCAAATCTCTCCCCCCCTCTCTCTCTTCAACTAAAACAAACACACCATTCACCTTCCCACATTCAAACCAAACATAACACACGAATGGCAATTGAAAACCAAGACAACACCTCTGTCAGAGAAATCAAACCAAAAAACAGAAGAATCATGGGTGCTGGAGGTCCTGATGAAGAGGATGACAGTAGATGGCCACCATGGTTGAAACCTTTGTTGAAAGAAAATTTCTTTGTTCAATGCAAGTTACATGCTGATTCCCACAAAAGTGAATGTAACATGTATTGTTTGGATTGTATGAATGGTCCTATATGTTCTCTCTGTCTTTCTTACCATAAAGAACATCGTTTTATTCAGATCCGAAGATCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAGGTATTGGACATCACTGGTGTTCAAACGTATATTATCAACAGTGCAAGAGTTGTGTTTTTGAATGAAAGGCCTCAGCCAAGGCCGGGGAAAGGTGTCACAAACACTTGTGAAGTTTGCGAGCGTAGTCTTCTCGATTCCTTCCGGTTCTGCTCTCTGGGTTGCAAGATTGTAGGAACATCTAAGAATTTCCAGAAGAAAAAGAAACAAATAACAGCAATGGCATCAGATTCAGAAGATTCATACAGTAGCAACAACAACATTCACAGCCGTCACAAGAAAATCAAAGTTCAAAGCTTCACACCATCAACACCTCCTCCAACTTCTGTTAATTACAGAACAGCCAAGAGAAGAAAGGGAATTCCACATAGAGCCCCATTAGGGGATAGATAGTTGGTACTATTAATAGTTGTTATTTTATTTATTGCTATAATCGACCCTAGTGTACAAGTTAACTTTTAATTTTTTTTAAAAAGTTGTTTTAGGTGGTGTATATAGAC
TARGET=47,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0515
PRIMER_LEFT_PENALTY=1.047917
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=TCTCTCCTTCAAATCTCTCCCC
PRIMER_RIGHT_SEQUENCE=CTTTTGTGGGAATCAGCATGTA
PRIMER_LEFT=22,22
PRIMER_RIGHT=292,22
PRIMER_LEFT_TM=61.048
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=1.0523
PRIMER_LEFT_1_PENALTY=1.047917
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=TCTCTCCTTCAAATCTCTCCCC
PRIMER_RIGHT_1_SEQUENCE=AGCACCCATGATTCTTCTGTTT
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=61.048
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=1.1227
PRIMER_LEFT_2_PENALTY=1.119099
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=CGGGGACTTCTTCTCAAACTCT
PRIMER_RIGHT_2_SEQUENCE=CTTTTGTGGGAATCAGCATGTA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=61.119
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=1.1235
PRIMER_LEFT_3_PENALTY=1.119099
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=CGGGGACTTCTTCTCAAACTCT
PRIMER_RIGHT_3_SEQUENCE=AGCACCCATGATTCTTCTGTTT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=61.119
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=1.1808
PRIMER_LEFT_4_PENALTY=1.047917
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=TCTCTCCTTCAAATCTCTCCCC
PRIMER_RIGHT_4_SEQUENCE=TTTGTGGGAATCAGCATGTAAC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=290,22
PRIMER_LEFT_4_TM=61.048
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_00331 Ps_contig_mira-and-tgicl-ass_franssen_3627
SEQUENCE=CTTGTGATTTACTCTTACTTCACTTAATGTACCTTTCTATTAGATCTTATTTTGTGATTTTATTTAATTAATTAAGATGTTGATTTTCGGTTACTGTTGGTTTAGGTGCTCTGATTGCTGTTCATTCTATTCTGTTTCATTTTTGGGATAAGTTGGGAGCTGAAGTGAAAAAAGAGGAGAAAGAAGAAAAGCTTGATGGTGATGCTGCTTTGAATAAATTGTTCCGTGATATTTATCAAAATGCAGATGAGGACATGAGGAGAGCCATGAGCTAAGTTCTTTTCNGGAATCAAATGGAACAGTGCTATCAACTGATTAGGAAAGAGGTGGATACGAAAGGAAGGTGGAAGGAACGTCCCTACCGAAGGAAGGAAGTGGGAGGTAGAA
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00332 Ps_contig_mira-and-tgicl-ass_franssen_3627
SEQUENCE=CTTGTGATTTACTCTTACTTCACTTAATGTACCTTTCTATTAGATCTTATTTTGTGATTTTATTTAATTAATTAAGATGTTGATTTTCGGTTACTGTTGGTTTAGGTGCTCTGATTGCTGTTCATTCTATTCTGTTTCATTTTTGGGATAAGTTGGGAGCTGAAGTGAAAAAAGAGGAGAAAGAAGAAAAGCTTGATGGTGATGCTGCTTTGAATAAATTGTTCCGTGATATTTATCAAAATGCAGATGAGGACATGAGGAGAGCCATGAGCTAAGTTCTTTTCNGGAATCAAATGGAACAGTGCTATCAACTGATTAGGAAAGAGGTGGATACGAAAGGAAGGTGGAAGGAACGTCCCTACCGAAGGAAGGAAGTGGGAGGTAGAA
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00333 Ps_contig_mira-and-tgicl-ass_franssen_3628
SEQUENCE=TGGTATCAACGCAGAGTCGGGGGGAAGTTTCAACGAACAAGTGAAGAAAGGGATTTGAAGAAGCAGAAGAAGAAAATCAGAGATGGCGAAAGAGCTTGAGAACAAAGCCAAAGAAGCGTTCTTCGATGACGATTTTTCTCTAGCCGTTGATCTCTATTCTCAGGCTATAGAACTCGATCCTGCCAACGCAAATCTCTTCGCCGACAGAGCTCAAGCACATATCAAACTCAACGCTTTCACTGAAGCTGTTTCTGATGCTAATAAGGCTATTCAATTAAACCCTACTTTATCAAAGGCTTATATCCGCAAGGGGACTGCTTGTATTAATCTGGAAGAATATCATACTGCTAAGGTAGCACTTGAAAAGGGTGCTTCGATTGCACCTGCAGATTCCAGGTTTACCAATTTGATTCAACAATGTGAACGCTACATTGCTGAGGAATCAGATAGTCTTACTGGTATCCTACCAACAAATGGGCCCAAAACTTCTGTACCATCTGTTGATGATACCCATATATGTGATAAAGGTGATGGAACAGGAGCATCCAAAGAACCTCAAAGAGACAGTACCACATCTGAAACAAGCGAAGCTGCACAAGTCAGACCAAAATACAGACATGAATATTACCAAAAGCCTGAAGAAGTGGTTGTCACAATATTTGCAAAAGGAATACCAGCAGACAATGTTGTTGTGGACTTCGGTGAACAAATTCTGAGTGTTACCATTAATGTTCCTGGCCAACATGCATATCTTTATCAACCTCGATTGTTTGGAAAGATTATCCCAGCTAAGTCCAAAGTTTTGGTGTTGTCAACCAAAATAGAAGTTCGTCTGGCAAAAGCTGAAGCTATTAATTGGACATCTCTGGAATATTGCAAGGATGTGGTTCCCCAGAAGATAAGCGTGCCTACAATTCAATCTGAAAGGCCTACATATCCATCATCAAAGTCAAGGACTAAGGATTGGGATAAGTTGGAAGCTGAAGTGAAAAAAGAGGAGAAAGAAGAAAAGCTTGATGGTGATGCTGCTTTGAATAAATTGTTCCGTGATATTTATCAAAATGCAGATGAGGACATGAGGAGAGCCATGAGCAAGTCTTTTCTGGAATCAAATGGAACAGTGCTATCAACTGATTGGAAAGAAGTGGGATCAAAGAAGGTGGAAGGAAGTCCTCCAGAAGGAATGGAGGTGAAGAAATGGGAGTATTAGTTCAGTTTCAATTACTGGGTCATATAAGCTTATGCTTTTGAGTTAAAATTCATTTAGCTTTTGTTTTTTGCATAGCCAATTTGTCTGCAATCTCTTGTCATTAGTTTCTATTTATTTATTTTTGTATGATTAAGTTTTATTTTTCCGTTGGGGTAAGTAATTKKAACTTAATTTGCTAGTTTCGGGACCGGGGTAAGTTTAGTTAAATTTTTTATTTATCTTAGGAACGACC
TARGET=1319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00334 Ps_contig_mira-and-tgicl-ass_franssen_3629
SEQUENCE=TTGTTCTTTATTTSYTAWCTWWSTKATTGGAAAGAAGTGGGATCAAAGAAGGTGGAAGGAAGTCCTCCAGAAGGAATGGAGGTGAAGAAATGGGAGTATTAGTTCAGTTTCAATTACTGGGTCATATAAGCTTATGCTTTTGAGTTAAAATTCATTTAGCTTTTGTTTTTTGCATAGCCAATTTGTCTGCAATCTCTTGTCATTAGTTTCTATTTATTTATTTTTGTATGATAAGTTATTTTCCTGGGAGAATTGACTAATTTGCATTTGCCGGGGAAGTGAATTTTATTTATTCTCTTGGTCTTTGTTGATTATAGTTATCGGTTAACTTCTGATAATACCCAGACTTGTTTTTATTTTATATTGACATTGGAACTTATATCTTGTAAATTTGACTGMAAAAA
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00335 Ps_contig_mira-and-tgicl-ass_franssen_3647
SEQUENCE=AGTTGTTCAAGCTTTTGAGCAACTTCAGCCATGTCTGATGATTTCACCTTGTAACCAACCACCGCTAAAAGCTCATCCATACCACCGTCGTCTTCCCAACAAATCTCCGATTTACCAGTCATACTCGCATCTTCGTGTTCTTGGTTATGCTCTCTCTTCATCTTCTTACACTTCTCTCTCTCTCTCACTCACACACAAGTTCTATATTCTGTTTTCTGATTGTAATTTGTGACTTTCTCTTTTCTTTCTCTGTCTTTTTTTTTTTTCTCACTACTGCTTCTAAACTTTCTTCATCAACACAATCATATACTCCGTMAAACTCATATATAT
TARGET=173,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00336 Ps_contig_mira-and-tgicl-ass_franssen_3662
SEQUENCE=CGAAGGTTCTCGAAATCTGGTGGCGGAAACTACGTAAWWCAACCAAAAATCGGGAAAAATAGCAACCTTTGGATCAAGATTGTAATTTAGAAACTCACCTTCGGGCGCCGGCAAATCTCCTCCGACGGCCGCAAGAATCAACATGATCCAACTCCTCCAATCTACGTCGTCATCATTGCTATGTTGGGAAGAACTCGAAGATACTTGTGTTGGCGATGGAGAGAGAATGGTTGTCTGGTTCGTATGCGTCGCATAGAGATGAAGATGAAGGTCGTCTGGACGTRGACGGGTAAGCTCTCTCTCGATTCTCGTTTCCGAWTTTTTGTTTCAGATTCCTTCGCTTTTCCTTTTCGATTCCGTTGCTCTTCTTCTTCTATTTTTTTATTTCTGTTGCAGGGTGGTGGATCATTGTGTTGGAGGTTGTTGTTGGAGATAGAATGGAGTTGTCGTCGATGGTGATGGATGACAGTGGATGAGTCTCGGGTGAATCTGGAGTAACCGAAGGTGAAGAAACCACGCTCAGGAAGTTTGAAGCAGTGGTTATTGAAGAAGGTCGGAGGGTTATGAAAATTTCATATGATGTTGTTGTAGAAAAAAACGGATTGGTTATAAGGAGGTGGAAGTTAGTTCGTGGAATGAATGCTTTGGCAACTGATCTCGTTTAGCTTCTCAATTCTATTCCTGTTCTTTTTTTGCTGTTGTTTCTGTTATGTTTTTTTATTTATTTCTACAGGTTATGAGCTCGAAAGAGTAGTAGTTAGCGAAACTCCGGAAGCTGGTTTTTAGTAGGAATTGA
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00337 Ps_contig_mira-and-tgicl-ass_franssen_3679
SEQUENCE=CTAAAAACCACCATCTATGCGAAACCACCACCTTGATAGTAACCCCGACAAAACGAAAGAAAATCGCACAAGAAGAAGAAGCAGAGATTAGGACAAGAAGAAAGCAGAGCAAGGGGAAACGATAAGGTATACCTTATACACTGCAGATTCGGTTGGTTTATGCGCTTTGCCTTTGTATAGATCCATGACTCTTAGGGCATTTTGATTTTGTTTTGATGTATGGTGATAACTGTTGATTTATTTTTTACATTTGTTGGATGAATTGTGTTTGTTGCAAACCATGTGAGGTTTGATTCCGACTTTGAAGGTACTGAGAACGAAAATCTGGAGTTCGGTTTTAACATGCGGAATTTGTTTCGCCTTTAAGGACTCTGATCCTTCCAATTGTTGTTCATTTAACGTTAGAATCATGTCTGGTTGGGGATTTGGGTATAGGATCTCTGTTCAGTAGAAGATTTTAGGAAAGTTGGTTCTTAGACTTTGTGTTTGTGGGTTCGGTTGTCCGGAGAAAATGGTGGTAACCACCGCAGACCACCGACTTTTTGTTCTTCTTCCCCGGAGAGGATTCAGAATGAGGGAGAAAGTATCCTAACGATAAGAAGGGAGGCACAATTAGGGAAGAGAAAGAATTGTTTGGTCTTTTAAGTCTGAATTTCATTTATCTTAATTACAADAACS
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00338 Ps_contig_mira-and-tgicl-ass_franssen_3681
SEQUENCE=TTGGTATTCGAATTTACTGTTGCTGTACATCGATCTCCGTTTTCTGTTTGGATTTGCAGTGGTTGATGTGGAGAGGAGGTGGACGATGGTGCGATTGGTAGAGTTGTTGTAGACTGGAAATGGACGTTTGATTGGTAAGGTTTTTGTTTAAAGGTTGAAAGTTTTGTTACGGTGATGACTGGTTGGAAGAAGAAGAAGAATCTGGAATCGTTGCAGAGTATTTGGAGAGTGAAAGAATGAAGAAGAAAAAGG
TARGET=186,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4876
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.483253
PRIMER_LEFT_SEQUENCE=ACATCGATCTCCGTTTTCTGTT
PRIMER_RIGHT_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT=26,22
PRIMER_RIGHT=228,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.517
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.5135
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.483253
PRIMER_LEFT_1_SEQUENCE=TGTTGTAGACTGGAAATGGACG
PRIMER_RIGHT_1_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.517
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.5922
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.483253
PRIMER_LEFT_2_SEQUENCE=AATGGACGTTTGATTGGTAAGG
PRIMER_RIGHT_2_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=228,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=59.517
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.6131
PRIMER_LEFT_3_PENALTY=0.129818
PRIMER_RIGHT_3_PENALTY=0.483253
PRIMER_LEFT_3_SEQUENCE=AGACTGGAAATGGACGTTTGAT
PRIMER_RIGHT_3_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=228,22
PRIMER_LEFT_3_TM=59.870
PRIMER_RIGHT_3_TM=59.517
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.7117
PRIMER_LEFT_4_PENALTY=0.228464
PRIMER_RIGHT_4_PENALTY=0.483253
PRIMER_LEFT_4_SEQUENCE=GACTGGAAATGGACGTTTGATT
PRIMER_RIGHT_4_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=60.228
PRIMER_RIGHT_4_TM=59.517
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_00339 Ps_contig_mira-and-tgicl-ass_franssen_3688
SEQUENCE=ACTTTTTAAAACTTTTACTATATCTTCWWTTTCCGCACTTGGTTTTTCTCACATATATACAATCTTGCAGAAAATAATTTAATGCTAACCAAATAAAAAAATAATTACTAGCTAATTAGCCTATGGAGACACTCAAAATCAGTGTTTTCACAGCACATGTAAGGACAACAAAATAAGGAGAATAAATTTCAGGTCCTTCCAGGATAAAATCTTACTCTTTGCTTCCAACTTTCCGCTTCTTTCGTTTGGAAGTTGGCGTCGAACTGTAAACATAAACAAAAATATTAAAAACAGCTCAATTGCAGATGATAGCATCATTAAATTTCATGAATGAATGAATTTATAAGGTGATGGATACCTCCCATTTATAAACACATTTTCAGGTCATAATGTAGAATAGTTAACACACCCCTCAAATTCAGGACTGAACCTTTGAAGTGTGATTCAACCGATCAAGGATAGACCTTCGTAGCTACTTAGAATTTAACTTGGGCCTAACTTAACTTTACACAATTGACTTGTAAGATGAGGGAAATAACTCAAAAACTTTCAGGTCATATATCACCCAACGTAATACTGCCAGCACACACCCATCTGACACTGAATACTAGACTTTTGGTGTCTCATCCGATTGCAAGAGGCGCAACGCATCTTGGAAACTCTCATCCGATCACTCAAGTCACACTTCAGATATTCAATCTTTGGACTCGAGAGAGTTGTATTAATAGTATCATATTTGACGATAAGACCTGAAAATGTGTTTACATAAGTTGACTCCCCCGCCTTACAAGCTAATTTTGTAGGGTATGTAACCTAAATTTAAGACAAATAAATCAAAATCTCATTAGGTTTGTTCTCTTTTGTTTATATGGTTCTAGTGGAAATTTGCTGTATTCATTGATTATTGAATTCAATCAGTTGGTTAACTGCTACATATTTTACAGCTGCTCCAGAGGAAAATGGATCATATGCCATGTATCTATTTATAATAAAGCTCAAGTTCTAACCATGGAAAATAATTTGCACACTCAATCAGACAGTAATCATATATTTAGTCCATTCCGAAAATATTAGAGTTTGGCATAGGTACCTTGTTAGAATAGGTTGTATATCAGCACCAACAGTTTTACTAAGCTCTTCAATAGTTGCAGAAACCAACATTACTACATTTAAGTATTAGTTGCAAG
TARGET=327,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00340 Ps_contig_mira-and-tgicl-ass_franssen_3692
SEQUENCE=TACACTCACTCACTCACCACTAGCTTTCTACCATCCCCTTTTCCCTTTACTTAACTAGAAAAACCAAAAGTAACTATCAATTTGTAAGAACTCTTCTTCTTTCACTATACATGATGATCAATCAGTCTCTTTCACAGTCCTGCATCAAACGGCACACCACTACCAGAAATCCATGACTCACCAGCCAAGAAATTACCAACAGAAAATTTAACTGCTTCCGAAGGATTTGTAATTACATGAAAACCAGACCAATTCACTCTCCCATCTGTATTAGCACCATCACCAACATTCATATATTCTGCATAATAAAGTGTGCTCAAGGCAAAACCACCACTCCATGGAGCCCAACCTTGTGAATTTATAACACCGTCAATATTACTTTTCATTACAACCGTTCTTGAATACTTCTGCCACGGCCTTCCTAGATAACTTTTCACTGAATTTTGAGATGCTTTCAAATCACCTGCTGCGGTGATACGGCAGTTATGGATCACTATTCCGGTATTCTCGTTTGGATCTGTTCTTCCCTGTGCCGTTACGGTGTTCTGTTGGTTGCCCATAGGTTTTCTAACTAAGATGTTGCAGTTTTGAAGAACTGTAACGGCGTTRCCAAAGATGAAATCGACTGTTCCGTAGATGTTACAGTCACGGTAGAATTGACGGTTGGCATAGACGTATAGAGTGTCCTGATAGCCTTTGAAGGAACATCGGTAAGAAAACGGAGTGATCGGCG
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00341 Ps_contig_mira-and-tgicl-ass_franssen_3699
SEQUENCE=GAATCTAAATGAAAGTGAGTTATGAGAATGAAAGGAAGGAACCRATGARAGGAGGAACATCATGTTTGCTGCTGCTGCAGCAGCTGTTTGTTCTTGTTGCTGGGATGGTCTTTGGCTGATGAACCTAACGTGGTTCTCCAGAAGACTAAAGAAAGCATATGCCACCGTTGTTTGGTACGTTACCA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00342 Ps_contig_mira-and-tgicl-ass_franssen_3704
SEQUENCE=TAAATAAGTACTAAACAAACAAACTAACCACTAAACCTTACCATCCAAATTAACAAAGTAAAGGTAGCTCAAACAATAGCTATGTTCATGGAATCATGTAAAAAAACTTTTAATAGTCAATTTATTGAATGAAACAAAGTAACCATTTGATTAACGAGCATTCAGGAACACAGATTCTTCAAGTTCTGATGTTCCTGTTTTTGTAGCTGGAGCCTAAGAAAATCAATGGCCGCTTCCGTGATCATCATGACCCCTCCCAGGGATGCCCTCCAGCCCAGTACGTACGGGAGCATTCTTGTCTTTCGCCCTGTACATACCAACCCCAAAACCACATGACAGCGCACAACGTCCGTTTGGCCGGTAGGTACGGTGTCGCCTAGTCGTCGTTTAGGTTG
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00343 Ps_contig_mira-and-tgicl-ass_franssen_3800
SEQUENCE=GAGTTGTGAGGAGAAAAAAGAAGAGAGGKTTAATTATTTATGAAGAAAATAAAGAGAGAGTAAGATTGGATTTATGAAGAGAGAGAGGAGTGTGGGTATAGTATRKYMMSSTAGA
TARGET=78,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00344 Ps_contig_mira-and-tgicl-ass_franssen_3802
SEQUENCE=CCTCTCTCKMWYCATAAATCCAATCTTACTCTCTCTTTATTTTCTTCATAAATAATTAAACCCCTCTCTCTTTTTCTCCTCACAACTCAACTGTTACCATTTTCTTCTTCTTCTTCTTCTTCCTTCCACCCTCAATATGGATTGCTGCATAATCAGTAAACTCAAATCCGAACCTCTCTGGTTCCTCCTTCTCTTCTCACTCGGTCTCACCACCATTCTCAGATTCACTCTAACATTCCTCAACTGGGTCTATGTTAATTTTCTCAGACAACCCAAAAACCTACAAAAAGTACGGATCTTGGGCACTCGTTACCGGATTCTTCTGGTTTAGG
TARGET=102,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00345 Ps_contig_mira-and-tgicl-ass_franssen_3805
SEQUENCE=GGTAGTCAACGACAGAGTAAACAACGGGGGAAGGTTAATTATTTAAAAACCCTAAAACGAATAAATAGCTCACCACCCCTCSGGTGTCATGTTCTTTTTCTAACTCTCTCTCTCTTTCTTCTCACCTTCCTTCTTTCTTCTTCTCCTCCTTTTCCATCTCTATATCTTCCATTTCTCCAATTTTCTCCTCCCTTATTGACCTTTGGTCACCACTCTCAAATACCCATTTCTCTATTTTCTGGTTTAAAGGTCTGAAAAAAGGTTGATAACTCTTTGAGTTGAGTTGGTGTTTGAAGCTAAACAAAGGTTTTTGAAACCCAATTTACAGGTTGAAGCTGATTTTTCTGGAAATTTGGGCCATCTTTAGCGGTGTTTTCCTGAGAAAAGATGATSATGTYWGTCTACGACACAAATATCACAGG
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00346 Ps_contig_mira-and-tgicl-ass_franssen_3811
SEQUENCE=ATTGAGGTAGTTAGATTTTTGCTTAGAGAAGTGTAGAGTAGGAACTAGTCTTGTTGTATTCCACAGTTTTCTCTTACTTTTCCAAGCTTTCGGACCATCTTGTCTTCACTACTATTTAGACCATCTCTAATAGAGGAGTTGTTGTTGTTTTATGTGTGTTTTTAAGCATAAGAATCTGTTGAGATGACATGTTGTGTTCTTCATATGTAGTAATAACATTCTTCAATAAAATAGTTGGTTCAAATTGWC
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00347 Ps_contig_mira-and-tgicl-ass_franssen_3812
SEQUENCE=CYAATTGAACCAACAATTTTATTGAAGAATGTTATTACTACATATGAAGAACACAACATGTCATCTCAACAGATTCTTATGCTTAAAAACACACGTAAAACAACAACAACTCCTCTATTAGAGATGGTCTAAATAGTAGTAAAGACCAGATGGTCCGAAAGCTTGGAAAAATAAAAGAAACCTGAAAACTGTGGAATACAACAAGACTAGTTCCTACTCTACACTTCTCTAAGCAAAAATCTAACTACCACAATGTCAAAGCTAATTTATTACAAATGATTGACCAAAATTCATACATCCCATGTTCACCTTCCTCTTTCTTTTTTTCTTTCTTTCTTTTAGTAAATCATGTCAACCAATCACGAACTCCTCGATATTTCTTTCAACAAGAAACTCTCACTGGAGTGATTGGTCGACGCTCGTCTGCTCATTTTATGTCTCTTCAAGTGCCGACAACACTGGCAGCAACTCAAGCATCATCACTGTTTCAACTTAGGTTTTGAAATGTTTTCAACTTACTTAAAGCTCCGGCATGCCATTTGATCGCGATGAATATTCTGTCACCAACTTCAAAAACATGGATGACTTGTCTTCTAATAGCCGCAATGGAGTGTCAAACTCTGCGTTGATACCACTYGTCTT
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00348 Ps_contig_mira-and-tgicl-ass_franssen_3812
SEQUENCE=CYAATTGAACCAACAATTTTATTGAAGAATGTTATTACTACATATGAAGAACACAACATGTCATCTCAACAGATTCTTATGCTTAAAAACACACGTAAAACAACAACAACTCCTCTATTAGAGATGGTCTAAATAGTAGTAAAGACCAGATGGTCCGAAAGCTTGGAAAAATAAAAGAAACCTGAAAACTGTGGAATACAACAAGACTAGTTCCTACTCTACACTTCTCTAAGCAAAAATCTAACTACCACAATGTCAAAGCTAATTTATTACAAATGATTGACCAAAATTCATACATCCCATGTTCACCTTCCTCTTTCTTTTTTTCTTTCTTTCTTTTAGTAAATCATGTCAACCAATCACGAACTCCTCGATATTTCTTTCAACAAGAAACTCTCACTGGAGTGATTGGTCGACGCTCGTCTGCTCATTTTATGTCTCTTCAAGTGCCGACAACACTGGCAGCAACTCAAGCATCATCACTGTTTCAACTTAGGTTTTGAAATGTTTTCAACTTACTTAAAGCTCCGGCATGCCATTTGATCGCGATGAATATTCTGTCACCAACTTCAAAAACATGGATGACTTGTCTTCTAATAGCCGCAATGGAGTGTCAAACTCTGCGTTGATACCACTYGTCTT
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00349 Ps_contig_mira-and-tgicl-ass_franssen_3823
SEQUENCE=GGAGTAGAATAGTGTGACCACTTATTTGATACTTCCTTACGTTCCTGCTTCTTTTTTCTTAACTGCTCTAGGTGCTAGACCAATCGCTTCGGAGCTTTTCTATGATCCTTAGCCTCCTGCATGTATCGATAAACATCTCCCAAGGAACATCACCGACAAGCATCCAGTCACCGTCTTTATCCTCGTAAGTAATAACATATTCTGACCCATGAAGCAGATCTCTCAGCTTTGTTTCATTCAGCATCTGATTTCCATGAGATTCACATTGTCCTGAATATAACATCGACATAAGCAGTAACTTGATCCAGACACAAAATTTGCCGAAAATCATTAGAAAGACTATTTATTTTTCTTACGAAAAAATGAACCTATTCCTACCTACCTATGGTGAAACAGCTGAACATCTTCTCAAGAGATGAAGAGAGTTCTGAATATGCAGTGTAGTTCTTCAGATCTACTTTTCTCAAATAAGGAGCACCATCCATGCTAACCTTCACAAACACGGCGCCTCCTAATCCCAGTTTTCCATCAGCCTCTTCGGTGTTCTTTGAAGCAGTTGTCAAGGAATTTTTGCGAAATGATCTTATTGGAGGCCAACCAACAACCT
TARGET=374,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2375
PRIMER_LEFT_PENALTY=0.203367
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TTCCATGAGATTCACATTGTCC
PRIMER_RIGHT_SEQUENCE=GCTGATGGAAAACTGGGATTAG
PRIMER_LEFT=249,22
PRIMER_RIGHT=532,22
PRIMER_LEFT_TM=59.797
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.3739
PRIMER_LEFT_1_PENALTY=0.203367
PRIMER_RIGHT_1_PENALTY=0.170538
PRIMER_LEFT_1_SEQUENCE=TTCCATGAGATTCACATTGTCC
PRIMER_RIGHT_1_SEQUENCE=CTGATGGAAAACTGGGATTAGG
PRIMER_LEFT_1=249,22
PRIMER_RIGHT_1=531,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.4092
PRIMER_LEFT_2_PENALTY=0.375090
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CCATGAGATTCACATTGTCCTG
PRIMER_RIGHT_2_SEQUENCE=GCTGATGGAAAACTGGGATTAG
PRIMER_LEFT_2=251,22
PRIMER_RIGHT_2=532,22
PRIMER_LEFT_2_TM=60.375
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.5456
PRIMER_LEFT_3_PENALTY=0.375090
PRIMER_RIGHT_3_PENALTY=0.170538
PRIMER_LEFT_3_SEQUENCE=CCATGAGATTCACATTGTCCTG
PRIMER_RIGHT_3_SEQUENCE=CTGATGGAAAACTGGGATTAGG
PRIMER_LEFT_3=251,22
PRIMER_RIGHT_3=531,22
PRIMER_LEFT_3_TM=60.375
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.5533
PRIMER_LEFT_4_PENALTY=0.102129
PRIMER_RIGHT_4_PENALTY=0.451213
PRIMER_LEFT_4_SEQUENCE=TGAAGCAGATCTCTCAGCTTTG
PRIMER_RIGHT_4_SEQUENCE=GGATGGTGCTCCTTATTTGAGA
PRIMER_LEFT_4=209,22
PRIMER_RIGHT_4=482,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.451
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_00350 Ps_contig_mira-and-tgicl-ass_franssen_3837
SEQUENCE=TGCTAGATTGTGTTTTGTTGAAATAAAAATGTCATTGCCTTGAGGTTATATATATATATATATATTGTATGAGACAATTAAGGTCATTCTTATGTTTCATTGAGAGTGAAAATTCATAATAAAAAAGGAATAACTACTTTACTTTTACTGATG
TARGET=47,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00351 Ps_contig_mira-and-tgicl-ass_franssen_3840
SEQUENCE=GTAACTTAAGAGCAAGTGTGACATTAATACTAAAACCAAGGTTAGGGTCATCATGATCCTTAATAAGAATACACAAACATCTTTTACTCTTATTAACAACATCCTTAACACCAGTGCAACAATCTATGGTTGGTGTTTTGGACTGACCACTTACAAAAGGAAGACAATTGGCCAAACCTATGAGTTTGTCTGTGCATTCTGCTTTGTCTTGGTTCACATCTGAGGTTACAAGACCAAACATAAGGACTAGGAAAATCAAGGAAGAGAAGAGTGATGATAGATTTTTTGCACTTTTGTAAACCATTTTGATGATGAAAGAGTAGCTCAGAAAGTGAGAGAGAGAGAGGGAGAGACAAAACAAACAAGAAATGTGTGATATAGAATGGAAACACCTCCCCGACCACTGTCTTCG
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8981
PRIMER_LEFT_PENALTY=0.059316
PRIMER_RIGHT_PENALTY=1.838812
PRIMER_LEFT_SEQUENCE=TCTGTGCATTCTGCTTTGTCTT
PRIMER_RIGHT_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT=188,22
PRIMER_RIGHT=397,21
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.161
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=1.9185
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=1.838812
PRIMER_LEFT_1_SEQUENCE=ACACCAGTGCAACAATCTATGG
PRIMER_RIGHT_1_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=397,21
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.161
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=1.9873
PRIMER_LEFT_2_PENALTY=0.148509
PRIMER_RIGHT_2_PENALTY=1.838812
PRIMER_LEFT_2_SEQUENCE=TTGTCTTGGTTCACATCTGAGG
PRIMER_RIGHT_2_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_2=203,22
PRIMER_RIGHT_2=397,21
PRIMER_LEFT_2_TM=60.149
PRIMER_RIGHT_2_TM=59.161
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=2.0587
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=1.838812
PRIMER_LEFT_3_SEQUENCE=CTGCTTTGTCTTGGTTCACATC
PRIMER_RIGHT_3_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_3=198,22
PRIMER_RIGHT_3=397,21
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=59.161
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=2.0587
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=1.838812
PRIMER_LEFT_4_SEQUENCE=TGCTTTGTCTTGGTTCACATCT
PRIMER_RIGHT_4_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_4=199,22
PRIMER_RIGHT_4=397,21
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=59.161
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00352 Ps_contig_mira-and-tgicl-ass_franssen_3847
SEQUENCE=CCAATCATCCACAAAATTAGCTTGTCCTATATAAAAAATAAAAATCGAAATATATAATAAATTGGCTAAACCATCTTCTATCTAACCCAACTCAAATAAATCCATTTACACTACTCAACTAAAAAAAACTTTAAGTCCTTTTAATTTTTAAATGTAATAATAATCTTGAAAATTGAAATACTAGTATTATTATTATTACTAAATATTTTAAAAACCGAACCAGCAAAACTATGGATCGGGTTCAACAATGTTAAACTGTTCGTTTCAGAATAAAGCCTGAATCAAACGGTTTAAATAATCAAACCCTAGATATCGTCGGTTGATTCGATTTTTAGAATATCGATTAAAAGGTTACTTAATAGTTGAATTGTCTTCCGGTGTAGGTTTGACCAAAAGACCAACTAGCTGGAGCAGCATTGAATGAAACCACACTATGACCATTACTTGTGGTGACAACAAAGGAAAGACTTTGTCCATTAAGGTAAGAGTTACTTTGCCAATTTTCGTCCCCCCAACTTTCTTTGACATGGCTTGCCATCTTGTTCTTGAACCTTTTATGGCCACAGAATGTACATCACCAGCACCTCCAACATTTGTCACAAGTACTAATTGAGAAGAATGT
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7655
PRIMER_LEFT_PENALTY=1.370891
PRIMER_RIGHT_PENALTY=0.394568
PRIMER_LEFT_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_SEQUENCE=CATAGTTTTGCTGGTTCGGTTT
PRIMER_LEFT=5,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=58.629
PRIMER_RIGHT_TM=60.395
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=1.7727
PRIMER_LEFT_1_PENALTY=1.370891
PRIMER_RIGHT_1_PENALTY=0.401769
PRIMER_LEFT_1_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_1_SEQUENCE=ATTGTTGAACCCGATCCATAGT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=58.629
PRIMER_RIGHT_1_TM=59.598
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=1.9636
PRIMER_LEFT_2_PENALTY=1.370891
PRIMER_RIGHT_2_PENALTY=0.592680
PRIMER_LEFT_2_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_2_SEQUENCE=TGTTGAACCCGATCCATAGTTT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=58.629
PRIMER_RIGHT_2_TM=60.593
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=1.9636
PRIMER_LEFT_3_PENALTY=1.370891
PRIMER_RIGHT_3_PENALTY=0.592680
PRIMER_LEFT_3_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_3_SEQUENCE=TTGTTGAACCCGATCCATAGTT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=58.629
PRIMER_RIGHT_3_TM=60.593
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=2.1253
PRIMER_LEFT_4_PENALTY=1.370891
PRIMER_RIGHT_4_PENALTY=0.754440
PRIMER_LEFT_4_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_4_SEQUENCE=GTTGAACCCGATCCATAGTTTT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=58.629
PRIMER_RIGHT_4_TM=59.246
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_00353 Ps_contig_mira-and-tgicl-ass_franssen_3850
SEQUENCE=GATAAACAGAATTTCGAGCAGGAATTTCCAATATTACCCTTTTCTCTTCCTCAAAATCTCGTTTCGCAATAGCATCTTTTGATTCCCACAGAAACACGCGCTCTCTCTCTCTCTCTTTCTCTCACTTCACGATGGAGAAAGCAGAGAGCTCCACCGCCTCCGCCTCCACCTCCGACCAAGACTCCGACGAAAAGCACCGCACACAACACCACCTAACAGTTCCATCAGGTCTAACTCAACACGAGTTCGACTCACTCATCTCTTTCGTCGCTGAACACCACACCTACCTCATAGGTCCCGGCCAATGCTCCACCTTGCTCGCTCAACGAATCCACGCGCCTCCTGAAACCGTCTGGTCCGTCGTACGCAGCTTCGACAAACCACAAATCTACWATAGACTCAATCCAAACTCACAATCACAATTACAAT
TARGET=101,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00354 Ps_contig_mira-and-tgicl-ass_franssen_3867
SEQUENCE=GTCGGGGAACCCTAAACTAARYCSTSGAAACAATTGTAAATTCCATTCCCAACAAACAGGGGTACACCACACCACACCGCACCGCATGACCAACCTCGCCGTCCCCCTCCTCCTCCTAACGGCGGCGCTAACCCTCTCCGCCGAACCAACCGCCTACGAAGCCCTTCGAAACTTCAATTTTCCAGAGGGAATTCTCCCACAAGGCGTAACACGCTACGAACTCGACGAATCAAGTGGCAATTTCCGCGCCGATCTGAACGGTTCGTGCCGTTTCTCCTTACAAGGTTCGTATC
TARGET=64,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00355 Ps_contig_mira-and-tgicl-ass_franssen_3867
SEQUENCE=GTCGGGGAACCCTAAACTAARYCSTSGAAACAATTGTAAATTCCATTCCCAACAAACAGGGGTACACCACACCACACCGCACCGCATGACCAACCTCGCCGTCCCCCTCCTCCTCCTAACGGCGGCGCTAACCCTCTCCGCCGAACCAACCGCCTACGAAGCCCTTCGAAACTTCAATTTTCCAGAGGGAATTCTCCCACAAGGCGTAACACGCTACGAACTCGACGAATCAAGTGGCAATTTCCGCGCCGATCTGAACGGTTCGTGCCGTTTCTCCTTACAAGGTTCGTATC
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00356 Ps_contig_mira-and-tgicl-ass_franssen_3888
SEQUENCE=GGTAGAACATAATTAAAATTTTGACCAAACAATAGAAACTCAATAAGAATTGTTACATTATTACAATATATATATTGATTGTTTGTCCTACAAAATACAACAGTACTTCTTTTCTTCGTACGGTAATACTCGAAGCTCGCAGTAAGATCTGATAGCCATTGACAGACATTGTTTCTCGAGGTTTTCTGATTTCTGCACAAAGCTCTGCAAGTCATCCCTATGAGAAACTCTTTCAACCTGCAGTTGAAAAGGTTGTAGCACAGTTTA
TARGET=66,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00357 Ps_contig_mira-and-tgicl-ass_franssen_3907
SEQUENCE=AGTTTGGCACCTATCCAATATTTTWCCAAATAACCTTGGCTGAACTAGATAGAGTTGTCTACTCAGAACTTCTGTATGATAGCATCATCATCATCTANTAGTAATTTNCTCTACATTCAATAGCTAGAAACCATC
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00358 Ps_contig_mira-and-tgicl-ass_franssen_3914
SEQUENCE=TAAACAGCTATGTAACCGATCCAGTTAGACTCACGGTCCCAACTCTCACGGGAAAGCGAGTGAAGCAAGAAGGCTTCAGGAACAGAGACACGTGCAGTAGCGTAAAGGAAAGAAACAACAGTGTAGTGTGTTGGGTTTTCAAGCATGACTTTGTTAAAGAACGTGGATTTTCCGTAACGGCTTGAACCGCAGTAGATAGAGTGTTGGTCGTTGTTGAAAGCATCGTAGGTTGTTTGTATGAAGTCACCGCAACGGAGTATGAGGTTGCGGAGGTTGAGGTCTAGTGGGTTTAATAGTGTTTCCCAGTTGTTGCTTCCTAGGAGTTGGTTCCATGTGTCTTGGTTTGTTGTTGTTGTTGATGTTGTTGATAATGTTGGTGGTGGTGGTGTTGTTTCTGTGTCTGTTGCCATGTTTATGGGACTTTTTTTGTGTGATAATCCCATGTTATAGGGAGTGTGTCTGTCTGTTTTTTTGAGGGAGTGGATATATATTGTGAGGAGTTGATGAGGGAAGAGAAGTGTGTGGTGAGA
TARGET=344,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1369
PRIMER_LEFT_PENALTY=0.121635
PRIMER_RIGHT_PENALTY=0.015253
PRIMER_LEFT_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_SEQUENCE=ACCACCACCAACATTATCAACA
PRIMER_LEFT=152,22
PRIMER_RIGHT=383,22
PRIMER_LEFT_TM=59.878
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.1369
PRIMER_LEFT_1_PENALTY=0.121635
PRIMER_RIGHT_1_PENALTY=0.015253
PRIMER_LEFT_1_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_1_SEQUENCE=CACCACCACCAACATTATCAAC
PRIMER_LEFT_1=152,22
PRIMER_RIGHT_1=384,22
PRIMER_LEFT_1_TM=59.878
PRIMER_RIGHT_1_TM=60.015
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2024
PRIMER_LEFT_2_PENALTY=0.121635
PRIMER_RIGHT_2_PENALTY=0.080808
PRIMER_LEFT_2_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_2_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=59.878
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.2374
PRIMER_LEFT_3_PENALTY=0.121635
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_3_SEQUENCE=AAAGTCCCATAAACATGGCAAC
PRIMER_LEFT_3=152,22
PRIMER_RIGHT_3=423,22
PRIMER_LEFT_3_TM=59.878
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.2454
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.080808
PRIMER_LEFT_4_SEQUENCE=GTTGTTGCTTCCTAGGAGTTGG
PRIMER_RIGHT_4_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_4=305,22
PRIMER_RIGHT_4=411,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_00359 Ps_contig_mira-and-tgicl-ass_franssen_3914
SEQUENCE=TAAACAGCTATGTAACCGATCCAGTTAGACTCACGGTCCCAACTCTCACGGGAAAGCGAGTGAAGCAAGAAGGCTTCAGGAACAGAGACACGTGCAGTAGCGTAAAGGAAAGAAACAACAGTGTAGTGTGTTGGGTTTTCAAGCATGACTTTGTTAAAGAACGTGGATTTTCCGTAACGGCTTGAACCGCAGTAGATAGAGTGTTGGTCGTTGTTGAAAGCATCGTAGGTTGTTTGTATGAAGTCACCGCAACGGAGTATGAGGTTGCGGAGGTTGAGGTCTAGTGGGTTTAATAGTGTTTCCCAGTTGTTGCTTCCTAGGAGTTGGTTCCATGTGTCTTGGTTTGTTGTTGTTGTTGATGTTGTTGATAATGTTGGTGGTGGTGGTGTTGTTTCTGTGTCTGTTGCCATGTTTATGGGACTTTTTTTGTGTGATAATCCCATGTTATAGGGAGTGTGTCTGTCTGTTTTTTTGAGGGAGTGGATATATATTGTGAGGAGTTGATGAGGGAAGAGAAGTGTGTGGTGAGA
TARGET=375,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2024
PRIMER_LEFT_PENALTY=0.121635
PRIMER_RIGHT_PENALTY=0.080808
PRIMER_LEFT_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT=152,22
PRIMER_RIGHT=411,22
PRIMER_LEFT_TM=59.878
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.2374
PRIMER_LEFT_1_PENALTY=0.121635
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_1_SEQUENCE=AAAGTCCCATAAACATGGCAAC
PRIMER_LEFT_1=152,22
PRIMER_RIGHT_1=423,22
PRIMER_LEFT_1_TM=59.878
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.2454
PRIMER_LEFT_2_PENALTY=0.164560
PRIMER_RIGHT_2_PENALTY=0.080808
PRIMER_LEFT_2_SEQUENCE=GTTGTTGCTTCCTAGGAGTTGG
PRIMER_RIGHT_2_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_2=305,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=60.165
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=0.2454
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.080808
PRIMER_LEFT_3_SEQUENCE=TTGTTGCTTCCTAGGAGTTGGT
PRIMER_RIGHT_3_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_3=306,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.2454
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.080808
PRIMER_LEFT_4_SEQUENCE=TGTTGCTTCCTAGGAGTTGGTT
PRIMER_RIGHT_4_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_4=307,22
PRIMER_RIGHT_4=411,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00360 Ps_contig_mira-and-tgicl-ass_franssen_3934
SEQUENCE=ATCCCTACCTTAGGGTCCGAGTAACTTTACCTTTCCGTTGGTTCCTAGACTNCGGGCGNGTGGCTTCGTACTTGGCCCTCGTCCGTCCTTGTACATGTGCGCAATTTCTAGGCACAAAGTGGATCGTCAGGGTTGGGGTCCGGTCAACAATGAGCAAAATTGACAAAAAGGAACCTTGGAAATGGTTAGTGCTGGACTCCACTGTTCTTTGAGAATGTCGAGACATATGCTACCGTTGCTGTTGATGTTTGGGTGGAATACCTTGGTCCTAAAAGCAACCTTTGGTGGCTTGAAAGGATAGTCAGGAGGAAAATGTATCGAAACCAGGAAAACACCGCCTGTATACGGACTATCAGACGGTCCCATGATCGTGGCTTGCCAGTGGAACATGTCCTCAGCCACAGGACCTGCACTGCATGACGTTGGAGGATCCTTCTGCAAATCCTTGAGCTCCTTCAAGATACGTTTCGAAGCCATGATAATGGAAACTGAGTCGGGAGCGTGGAAGAAGAACGAATGATTGGGAATGAGGGTTGAAGAAGAAGAAAAGGAGAGATTTAGAAAAAATCGGAAAGTATTGAGTTGAATGTTCCCGCGTACTCTGCGTTGA
TARGET=536,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7376
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.730647
PRIMER_LEFT_SEQUENCE=AGATACGTTTCGAAGCCATGAT
PRIMER_RIGHT_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT=458,22
PRIMER_RIGHT=595,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.731
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=0.9145
PRIMER_LEFT_1_PENALTY=0.183834
PRIMER_RIGHT_1_PENALTY=0.730647
PRIMER_LEFT_1_SEQUENCE=GGAAAATGTATCGAAACCAGGA
PRIMER_RIGHT_1_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_1=307,22
PRIMER_RIGHT_1=595,22
PRIMER_LEFT_1_TM=60.184
PRIMER_RIGHT_1_TM=60.731
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.9384
PRIMER_LEFT_2_PENALTY=0.207756
PRIMER_RIGHT_2_PENALTY=0.730647
PRIMER_LEFT_2_SEQUENCE=GATCCTTCTGCAAATCCTTGAG
PRIMER_RIGHT_2_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_2=428,22
PRIMER_RIGHT_2=595,22
PRIMER_LEFT_2_TM=60.208
PRIMER_RIGHT_2_TM=60.731
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.9502
PRIMER_LEFT_3_PENALTY=0.219551
PRIMER_RIGHT_3_PENALTY=0.730647
PRIMER_LEFT_3_SEQUENCE=GACTATCAGACGGTCCCATGAT
PRIMER_RIGHT_3_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_3=347,22
PRIMER_RIGHT_3=595,22
PRIMER_LEFT_3_TM=60.220
PRIMER_RIGHT_3_TM=60.731
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.5000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.9840
PRIMER_LEFT_4_PENALTY=0.253332
PRIMER_RIGHT_4_PENALTY=0.730647
PRIMER_LEFT_4_SEQUENCE=GTATCGAAACCAGGAAAACACC
PRIMER_RIGHT_4_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_4=314,22
PRIMER_RIGHT_4=595,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=60.731
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_00361 Ps_contig_mira-and-tgicl-ass_franssen_3937
SEQUENCE=AAAGAAAGGTTTCCCTTTCTTTTGGATTGGAAGCTTCACTGGAAGGCTTAGAGCCAGGGTATTTCCATTTCCAACGGCAGGCACGCTCACAACACACACACACTCGCACTTTTGAATCTGATTATGTTTTTTCTTTCACTATTGTACA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00362 Ps_contig_mira-and-tgicl-ass_franssen_3944
SEQUENCE=CGTNTGGCAGTGACACCAAACCATCACGTTGCTTCTATCGTGGCTGCTGCATTTTATGCAATTTGGAACCNCTTTTCAGGGTTCTTCTTACCTTTTGATAACAGAGCATTCCAGTATGGTGGAGATGGTACTACTGGGCATGTCCAGTTGCATGGACCATCTACGGATTGGTTGCATCTCAATTTGGAGACATAACTACTCGTATGACTAGTGAAAGCGGAAAGGAGGTGAAATTTTTCCTTGAAGACTATTTTGGTATCAAACATGACTTCATAGGAGAGTGCGCAATTGTGGTTGCCGGTATTGCTGTTCTCTTTGCATTTATTTTTGCTGTTGCAATCAAGTCCTTTAACTTTCAAAAGAGATAGATTGTACTCTCTCTCTTTCTCCAACAAGAATTTCTTTATTTATTTTTTACTTTTCCCCTCCCCCTCTACTTTTCCTTGCTTGTATAATCTTGTATCTTGTTATTTCATACTAGTTAGTTTTTAAGGTAATTTACTTTTCCCCTTTGTTTGACACTAA
TARGET=375,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5638
PRIMER_LEFT_PENALTY=0.102129
PRIMER_RIGHT_PENALTY=0.461713
PRIMER_LEFT_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_SEQUENCE=AGTGTCAAACAAAGGGGAAAAG
PRIMER_LEFT=263,22
PRIMER_RIGHT=522,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=59.538
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.6912
PRIMER_LEFT_1_PENALTY=0.102129
PRIMER_RIGHT_1_PENALTY=0.589035
PRIMER_LEFT_1_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_1_SEQUENCE=GTGTCAAACAAAGGGGAAAAGT
PRIMER_LEFT_1=263,22
PRIMER_RIGHT_1=521,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.411
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=1.1450
PRIMER_LEFT_2_PENALTY=0.102129
PRIMER_RIGHT_2_PENALTY=1.042913
PRIMER_LEFT_2_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_2_SEQUENCE=ACAAGCAAGGAAAAGTAGAGGG
PRIMER_LEFT_2=263,22
PRIMER_RIGHT_2=450,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=58.957
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=1.1542
PRIMER_LEFT_3_PENALTY=0.102129
PRIMER_RIGHT_3_PENALTY=1.052069
PRIMER_LEFT_3_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_3_SEQUENCE=AAGGAAAAGTAGAGGGGGAGG
PRIMER_LEFT_3=263,22
PRIMER_RIGHT_3=444,21
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=1.2595
PRIMER_LEFT_4_PENALTY=0.216588
PRIMER_RIGHT_4_PENALTY=1.042913
PRIMER_LEFT_4_SEQUENCE=ATGACTAGTGAAAGCGGAAAGG
PRIMER_RIGHT_4_SEQUENCE=ACAAGCAAGGAAAAGTAGAGGG
PRIMER_LEFT_4=203,22
PRIMER_RIGHT_4=450,22
PRIMER_LEFT_4_TM=59.783
PRIMER_RIGHT_4_TM=58.957
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_00363 Ps_contig_mira-and-tgicl-ass_franssen_3964
SEQUENCE=GATCAAGAAACAAAGTAAAGGTGTGAACGTGAACTGAAAATGATGAAGAAGAATTCAGTGGTTTCTATGATGCTTTTAGTATTTCTTATAGTATTTTCTCAAATGGATAACGTTGTTCCAGATGCTTCTGATTGCTTAGATGGTTGCTCCACTGCATGCGTTTCAAGAGACTGTTTGTTACTCTTCTCAACCTTTTATTTTACTTATACTTAATTAATTAATTTTCTTCTTCTATGTTTTCTATTTTCTTATTTCTTACTACTAATTAAAATTTAT
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00364 Ps_contig_mira-and-tgicl-ass_franssen_3969
SEQUENCE=TTGCTGCATGTCACATAAATTTTAATTTAGTAGTAAGAAAATGAAAATAAAAAACATAGAAGAAGAAAATTAATTAATTAATTAATTAATTAATTAAGTATAAATAAAAGGTTGAGAAGAGTAACAAACAG
TARGET=68,28
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00365 Ps_contig_mira-and-tgicl-ass_franssen_3975
SEQUENCE=GCCAGCATAATCCACCAGCTTTAAATCGTCCATTATAGTGTATTCTAATTCAAATTAACWCCACCACCTTCACATTGAGTACATAATATTGCACCATTTCCATCACAAATACAGGAACAGAACAATGCTCCTAACTTTTGTTAGCTGGAGTACTATCTCCTGTTGTAGCCTTAACTTCCAAAGACTCAAAGTTTCTAACTTTAGAATTCAGACAAACGTCCTTCTTCTAAAAAGAACCTTTCCGGGTCCGAACCGAAACTTAACCCGTATAAAGAAGGTTCCGTACGGTTGGTTGGTTTTGGTTAAAGG
TARGET=287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00366 Ps_contig_mira-and-tgicl-ass_franssen_3983
SEQUENCE=CCGTAATAAATNAAAAGGACTCTGAGGCAATTGGGCTAGGTAAAATTATAACTCAAAATACATTTTTAAACTATTATGGTTATAMAAGTAAAGAACATCTTATATTAAACTATTATGGTTAATTTGTAAATTACAGCGCCAAGGGCAGTGGAAAAGTGCAAGAACAGAAGCTGAAAACCTCGTTATCATGTTCCGACAAAAATCCTCTTGTTGTTGATCATGTATTGGCGTTCCAAGGAAGCAAATTGGGCTCGAGTAAGATCGGGGCTAATATAGGAATAATAAGAGTTCAATTATTATTATTATTATTATTGTTATGTGTCTGTGTTATTTCTTTTGTATTTTTTTTCTTCTTATATGTGTATTATATATGAATGAGAAATTAGGTGGAAATTCGTTAGGGACAAGAAATAATATATAAGATGTTTATATGACCCACTTTTTGTTTTCTAAGGAAAAAAAAATGTGACTTGTCTATTGCCAGCGTGTGCATGACTTATAATAAATGTCAATTTTCCAGACTATMAAACT
TARGET=293,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00367 Ps_contig_mira-and-tgicl-ass_franssen_4000
SEQUENCE=CTAGTGAAAAGCAGTTACATAATTTTAAGCAAATAAAGGAAGCGTTGGATTTGGATTACATTTTGGTACATAATATAACATTTATAAAGATAAAAATAAAATTAAAAACAGGTCACCAGTGCTAGATTCATCATTCATGTTATGGTAACAATCTCAGCATCTGATTCAACCAATTTAGTTCTTTCTTTTTTTGAAAATAGTACTAGTAATAATAATAATAATAATAATAAATTAAAAAAAAAACATGAACTAAGAAGAAAGTGGCCTTGGTGGAGGAGGAAGTAGTTGATGGAGTATTTGGAGGACTCTGCGTTGACTACCACTGCTT
TARGET=207,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3365
PRIMER_LEFT_PENALTY=0.312496
PRIMER_RIGHT_PENALTY=0.023981
PRIMER_LEFT_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_SEQUENCE=CAGTGGTAGTCAACGCAGAGTC
PRIMER_LEFT=108,22
PRIMER_RIGHT=324,22
PRIMER_LEFT_TM=60.312
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.5336
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=0.221115
PRIMER_LEFT_1_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_1_SEQUENCE=ACGCAGAGTCCTCCAAATACTC
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.7364
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.423908
PRIMER_LEFT_2_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_2_SEQUENCE=TCCATCAACTACTTCCTCCTCC
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.576
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.7997
PRIMER_LEFT_3_PENALTY=0.775718
PRIMER_RIGHT_3_PENALTY=0.023981
PRIMER_LEFT_3_SEQUENCE=TCTCAGCATCTGATTCAACCAA
PRIMER_RIGHT_3_SEQUENCE=CAGTGGTAGTCAACGCAGAGTC
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.776
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.8001
PRIMER_LEFT_4_PENALTY=0.312496
PRIMER_RIGHT_4_PENALTY=0.487583
PRIMER_LEFT_4_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_4_SEQUENCE=CCATCAACTACTTCCTCCTCCA
PRIMER_LEFT_4=108,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=60.488
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_00368 Ps_contig_mira-and-tgicl-ass_franssen_4002
SEQUENCE=TCCTCCAAATCTCCATCAACTACTTCCTCCTCCACCAAGGCCACTTTCTTCTTAGTTCATGTTTTTTTTTTAATTATTATTATTATTATTACTAGTACTATTTTCAAAAAARRAAGAACTAAATTGGTTGAATACAGATGCTGAGATTGTTACCATAACATGAATGATGAATCTAGCACTGGTNACCTTGTTTTTTAATTTTATTTTTATCTTTATAAATGTTATATTATGTACCAAAGTGTAANCCAAATCCAACGCTTTCTTTATTTGTTTAAAATTNTGTAACTGTCTTTT
TARGET=73,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00369 Ps_contig_mira-and-tgicl-ass_franssen_4008
SEQUENCE=GACGTTCCGGTGAATATGCGGGAACTAGTTGACTGCATGAGAGATGTTCGAATTGCGGATTCGGGTCGGGTCGGGTGTCCGCAGGGTGGGTTTTTAGTCTTCCGGCAAGCTATGGAAGGGGTTAGTATGGAGAGGGTGGAATCTGGAAGAGATCTTAGAGCCAAAATCTATGGAAAATTCAGTAAGGTGAACTTCAACGACGGTTGTTGTTTCGGTTCCTGTTCCTGTATTTTGGATTGGGTTTCGGAACTGGTTAACTGGTAATTTTRGGGATTGGAGGTTGAGGAGTATGATTYYGCTTTGTATATAGGATGAATGATGACAAAAAGGAAGAAGTAAATTCGTTTTGGTGCTAAGTTAAGGTTTGGAAGGG
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00370 Ps_contig_mira-and-tgicl-ass_franssen_4010
SEQUENCE=GACGTCCACAATAAACTAGTTACATTGACAATCTGTCACCATGGCGGCTCATATTAAACACCAATCCCAATCCACTCTTCTCTCTCAATCTCACCCTCTTATAATTCCCTCACCACCACCACCATTTCTCCGATCACCCTTCTCTTCACTTTCCGGCGAGCCAAATCTTTTTATCACCATTTCCTACACTAAGTAACAACTCAACAAAAAAAACAAATCATGTACAAACGCAGATTAACAAACAAACTAAACCATTNTA
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2570
PRIMER_LEFT_PENALTY=0.226866
PRIMER_RIGHT_PENALTY=0.030114
PRIMER_LEFT_SEQUENCE=GCGGCTCATATTAAACACCAAT
PRIMER_RIGHT_SEQUENCE=GGTGATAAAAAGATTTGGCTCG
PRIMER_LEFT=43,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=60.227
PRIMER_RIGHT_TM=59.970
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.2846
PRIMER_LEFT_1_PENALTY=0.182682
PRIMER_RIGHT_1_PENALTY=0.101938
PRIMER_LEFT_1_SEQUENCE=TCCCAATCCACTCTTCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=GTGATAAAAAGATTTGGCTCGC
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=59.817
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.3288
PRIMER_LEFT_2_PENALTY=0.226866
PRIMER_RIGHT_2_PENALTY=0.101938
PRIMER_LEFT_2_SEQUENCE=GCGGCTCATATTAAACACCAAT
PRIMER_RIGHT_2_SEQUENCE=GTGATAAAAAGATTTGGCTCGC
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=176,22
PRIMER_LEFT_2_TM=60.227
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.4540
PRIMER_LEFT_3_PENALTY=0.423908
PRIMER_RIGHT_3_PENALTY=0.030114
PRIMER_LEFT_3_SEQUENCE=CAATCCCAATCCACTCTTCTCT
PRIMER_RIGHT_3_SEQUENCE=GGTGATAAAAAGATTTGGCTCG
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=59.576
PRIMER_RIGHT_3_TM=59.970
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=117
PRIMER_PAIR_PENALTY_4=0.4757
PRIMER_LEFT_4_PENALTY=0.226866
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=GCGGCTCATATTAAACACCAAT
PRIMER_RIGHT_4_SEQUENCE=AAAGTGAAGAGAAGGGTGATCG
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_00371 Ps_contig_mira-and-tgicl-ass_franssen_4013
SEQUENCE=GTACTTGACCCGATAGCTCCATACAAATTACAACACCTCTCTCTCTCTCTCTTCCATTCACGCTTCTCAACAAACTCTCTCTCTCCCATGGCGACTTCTTTCGCGCCATTATCCATTTCAGTTGGTGGATCTCATCTCAAGTCATCTCTGGTTCGACCTAACCTTGCTGTTCAAAGGAAATCAAACATTCTGACAAGAAAGAACCTCACTTCACCACTTCGTGCAGAATACCGTGATAACAGAGGAGGTGGAGGTGGGGAATTGCTTA
TARGET=37,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4966
PRIMER_LEFT_PENALTY=0.491057
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT=2,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=59.509
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.4966
PRIMER_LEFT_1_PENALTY=0.491057
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=GTACTTGACCCGATAGCTCCAT
PRIMER_RIGHT_1_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=255,22
PRIMER_LEFT_1_TM=59.509
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.5016
PRIMER_LEFT_2_PENALTY=0.491057
PRIMER_RIGHT_2_PENALTY=0.010545
PRIMER_LEFT_2_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_2_SEQUENCE=TCGAACCAGAGATGACTTGAGA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=155,22
PRIMER_LEFT_2_TM=59.509
PRIMER_RIGHT_2_TM=59.989
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.5016
PRIMER_LEFT_3_PENALTY=0.491057
PRIMER_RIGHT_3_PENALTY=0.010545
PRIMER_LEFT_3_SEQUENCE=GTACTTGACCCGATAGCTCCAT
PRIMER_RIGHT_3_SEQUENCE=TCGAACCAGAGATGACTTGAGA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=59.509
PRIMER_RIGHT_3_TM=59.989
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.5729
PRIMER_LEFT_4_PENALTY=0.491057
PRIMER_RIGHT_4_PENALTY=0.081801
PRIMER_LEFT_4_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_4_SEQUENCE=ACCAACTGAAATGGATAATGGC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=125,22
PRIMER_LEFT_4_TM=59.509
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_00372 Ps_contig_mira-and-tgicl-ass_franssen_4013
SEQUENCE=GTACTTGACCCGATAGCTCCATACAAATTACAACACCTCTCTCTCTCTCTCTTCCATTCACGCTTCTCAACAAACTCTCTCTCTCCCATGGCGACTTCTTTCGCGCCATTATCCATTTCAGTTGGTGGATCTCATCTCAAGTCATCTCTGGTTCGACCTAACCTTGCTGTTCAAAGGAAATCAAACATTCTGACAAGAAAGAACCTCACTTCACCACTTCGTGCAGAATACCGTGATAACAGAGGAGGTGGAGGTGGGGAATTGCTTA
TARGET=75,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1587
PRIMER_LEFT_PENALTY=0.153161
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_RIGHT_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT=42,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.153
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.4629
PRIMER_LEFT_1_PENALTY=0.153161
PRIMER_RIGHT_1_PENALTY=0.309780
PRIMER_LEFT_1_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_RIGHT_1_SEQUENCE=CCTTTGAACAGCAAGGTTAGGT
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=60.153
PRIMER_RIGHT_1_TM=59.690
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.4966
PRIMER_LEFT_2_PENALTY=0.491057
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_2_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.509
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.4966
PRIMER_LEFT_3_PENALTY=0.491057
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=GTACTTGACCCGATAGCTCCAT
PRIMER_RIGHT_3_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=59.509
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.5016
PRIMER_LEFT_4_PENALTY=0.491057
PRIMER_RIGHT_4_PENALTY=0.010545
PRIMER_LEFT_4_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_4_SEQUENCE=TCGAACCAGAGATGACTTGAGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=155,22
PRIMER_LEFT_4_TM=59.509
PRIMER_RIGHT_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_00373 Ps_contig_mira-and-tgicl-ass_franssen_4014
SEQUENCE=GTATACAACGCAGAGTACGCGGAGTGACCCGATAGCTCCATACAAATTACAACACCTCTCTCTCTCTCTCTCTCTTCCATTCACGCTTCTACAAACAAACTCTCTCTCCTCCCATGGCGACTTCTTCGCGCCATTATCCTATTTCTAGGTTGGGTGGATCTCATCTACAAGTCATCTCGTTGGGTTCCGACCCTTAACCTTG
TARGET=56,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1808
PRIMER_LEFT_PENALTY=0.010294
PRIMER_RIGHT_PENALTY=0.170538
PRIMER_LEFT_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_SEQUENCE=TCCACCCAACCTAGAAATAGGA
PRIMER_LEFT=21,22
PRIMER_RIGHT=157,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=59.829
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.1836
PRIMER_LEFT_1_PENALTY=0.010294
PRIMER_RIGHT_1_PENALTY=0.173354
PRIMER_LEFT_1_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_1_SEQUENCE=ATGAGATCCACCCAACCTAGAA
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=163,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=59.827
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.3378
PRIMER_LEFT_2_PENALTY=0.010294
PRIMER_RIGHT_2_PENALTY=0.327533
PRIMER_LEFT_2_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_2_SEQUENCE=GATGAGATCCACCCAACCTAGA
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=60.328
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.6833
PRIMER_LEFT_3_PENALTY=0.010294
PRIMER_RIGHT_3_PENALTY=0.672968
PRIMER_LEFT_3_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_3_SEQUENCE=GTAGATGAGATCCACCCAACCT
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=167,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=59.327
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.8118
PRIMER_LEFT_4_PENALTY=0.641309
PRIMER_RIGHT_4_PENALTY=0.170538
PRIMER_LEFT_4_SEQUENCE=GACCCGATAGCTCCATACAAAT
PRIMER_RIGHT_4_SEQUENCE=TCCACCCAACCTAGAAATAGGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=157,22
PRIMER_LEFT_4_TM=59.359
PRIMER_RIGHT_4_TM=59.829
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_00374 Ps_contig_mira-and-tgicl-ass_franssen_4039
SEQUENCE=GTATTTGGTAATAGNTTCNCAAGCCAGCTATAACAGAAATCAAATAGTTGGTTGGAGGTTTTGTCAAGAGATWCATGAAACAAGCAAACTTACTCTATCAGTAATTGATTTCAGATCCATTTGGTCGGTCAACGCTTGTTTAGAAAGAGCCCCTGCCTTGTCAATCTTGTTCCCCAACACCAATAGAGGGATACCACTCAATGATGGTTTGCTCAGCAAATCATGAAGTTCACTTCTTGATATGCTAAGATTATCTGGATCAGCAGCATCAACAACATAAACAATAGCAGAAACCGCACGGCAGTAGCGTTCCCACATGCTACGGAACCTAGGTTGCCCTCCAAGATCCCATAGCTTAATTGTAACATTGCCTTTTGTTACTTTCCTCATATTGAATCCCACGGTAGGAATCATGTCCTCGCTGTATCCACCGGTAGCAACAACATTTACGAAGGGAAGTCTTTCCAGCATTCTGCAGTCCTATAAGAGATAACTCCATTTCCTGCTTAGAAGAAAGGCTGACGTAACCAATTACAAAAAGCCTTCCCACAACTCCCTATTTCGTTCCTTTTTACCCGTAACTTTAATTTCCTTTTCGTTCCTTCCAACCAACCAAACCCTCCCTTCCCTCTTCCCG
TARGET=605,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00375 Ps_contig_mira-and-tgicl-ass_franssen_4052
SEQUENCE=GGGGKAGAATCGTACCTTCAAAATTTTCCCAAAATGAGCGATTGTGGTTGTGAGACCTCACAGACCAACTGAAGCCGTCATGGAGGTTGCGCCGGTGAGGAGCGTTTGAAGGTTGATTGTGGCCGGCGGAGAAATTGTGGTTTTGTGAGGGTGCTCGTTGGAGGTAGAAAATGAGGGTGTGGAACGTGTGGGTTCGCGGTCGAAGGAGGAGAAGAGGTGCGTGTTTGGAGGTTTGGAAGGATTCGTTGGAAGAAGAAGAAGATGAAGTTGAGTTTGATGCGTCCCGTCTTCAGATAAGCCTCCTTCTTCTGTTTCGGTTTTTTTTTAGGGCTTTTTGAAAATATTGCTCTTTGTTGTTGTTCTTGCTGTGCGTTTTCTGTTGTGTACTGGTTTAGGGTTTTGAGAAAAATATCTTTGTTGTGTATCCTCTTGCTGTTAACTTTTTAGGGCTCTTAGAAAAAATTGATTTGTTCTTGCTGTTGTGTTGTTTAGGGTTTTAGAAAAAATCTCTGTGTTGTTGCTGTTGTTCTTTTGGTTTTTTTTTAGAAATAATTTGTTACTGTGTTGTGTTCCTGTTAACCTCTCCTAATCCATTTTTGAAGACAAGATTCAGACTTTAAAAACTCTTTGCTTTTTGAATTTCCCATCCCAGATTATGTGGGATTTGGTTTATTCTGTTTTAAGCTAATGCCAAAAGTGCTTTTTGGATGTTTATGTTAACCTTATGGATAAAGCTTCAAACTGAGTAATTTAAGTTTAAAGTTATTTTCAGTTTTTAAGCGCTTTTGAAAAGTGCT
TARGET=249,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00376 Ps_contig_mira-and-tgicl-ass_franssen_4053
SEQUENCE=GCCTGCATAACCACCAGCACCACCACTGCCCCCATAACCTCCAAAACCAAACTCTTCAGAACTTTTCAAAAACCTGTTGAATAACAACCCCCTTTTTCAATCAATCAACATCAACATCACTTCACCTGACAAAAAACCAATTACAAAAAAAACAGCTTATTCAAAAAGCACTTTTC
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5309
PRIMER_LEFT_PENALTY=0.717980
PRIMER_RIGHT_PENALTY=0.812957
PRIMER_LEFT_SEQUENCE=CCATAACCTCCAAAACCAAACT
PRIMER_RIGHT_SEQUENCE=TTGTCAGGTGAAGTGATGTTGA
PRIMER_LEFT=31,22
PRIMER_RIGHT=131,22
PRIMER_LEFT_TM=59.282
PRIMER_RIGHT_TM=59.187
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=2.0433
PRIMER_LEFT_1_PENALTY=0.717980
PRIMER_RIGHT_1_PENALTY=1.325343
PRIMER_LEFT_1_SEQUENCE=CCATAACCTCCAAAACCAAACT
PRIMER_RIGHT_1_SEQUENCE=TTTGTCAGGTGAAGTGATGTTG
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=132,22
PRIMER_LEFT_1_TM=59.282
PRIMER_RIGHT_1_TM=58.675
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=2.4367
PRIMER_LEFT_2_PENALTY=1.623715
PRIMER_RIGHT_2_PENALTY=0.812957
PRIMER_LEFT_2_SEQUENCE=CCCATAACCTCCAAAACCAAAC
PRIMER_RIGHT_2_SEQUENCE=TTGTCAGGTGAAGTGATGTTGA
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=131,22
PRIMER_LEFT_2_TM=61.624
PRIMER_RIGHT_2_TM=59.187
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=2.6598
PRIMER_LEFT_3_PENALTY=0.717980
PRIMER_RIGHT_3_PENALTY=1.941852
PRIMER_LEFT_3_SEQUENCE=CCATAACCTCCAAAACCAAACT
PRIMER_RIGHT_3_SEQUENCE=TGTCAGGTGAAGTGATGTTGAT
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=130,22
PRIMER_LEFT_3_TM=59.282
PRIMER_RIGHT_3_TM=58.058
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=2.7279
PRIMER_LEFT_4_PENALTY=1.914963
PRIMER_RIGHT_4_PENALTY=0.812957
PRIMER_LEFT_4_SEQUENCE=CCCATAACCTCCAAAACCAAA
PRIMER_RIGHT_4_SEQUENCE=TTGTCAGGTGAAGTGATGTTGA
PRIMER_LEFT_4=30,21
PRIMER_RIGHT_4=131,22
PRIMER_LEFT_4_TM=60.915
PRIMER_RIGHT_4_TM=59.187
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_00377 Ps_contig_mira-and-tgicl-ass_franssen_4057
SEQUENCE=CTCTYTTTGGAAGTTCCCAATTCAAAAACCAACTCTYCAGAACTTTTCAAAAACCTGTTGAATAACAACCCCCTTTTTCAATCAATCAACATCAACATCACTTCACCTGCAAAAACCAATTCAAAATCAAAAAACAGCTTATTCAAAAAGCACTTTTCAAAAGCGCTTAAAAAACTGAAAAATAACTAAACAAATTACTCAGTTTGAAGCTTTATCCATAAGGTTAACATAAACATCCAAAAAGCACTTTTGGCATTAGCTTAAAACAGAATAAACCAAA
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00378 Ps_contig_mira-and-tgicl-ass_franssen_4061
SEQUENCE=GATTCACGTTCATCTCAATGTCAGCTCTACACACTCCCTTCACTCACATTCCCTACAATCATTATTCAATCTTCACAAATAGGCTTTATTACGCTTCTACATTTCCCCCTCCAATAACACTCCACCACCTCCCCAAATTAACATTCTCTGCTTCTTCTTCTTCTGTTCTCCCTACTGTTGTTGATAATGGAAGCCACAACGTCGAACAAGATTCGAAGAAAGAGAATCAATTTGTGGATGAAAAAGAGATGACGAGAAGAATGAGAATAGGACTAGCTAACAAAGGAAAAGCACCTTGGAATAAGGGAAGGAAACACACTGCAG
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0544
PRIMER_LEFT_PENALTY=0.050763
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_SEQUENCE=TGGCTTCCATTATCAACAACAG
PRIMER_LEFT=20,22
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=60.051
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.1612
PRIMER_LEFT_1_PENALTY=0.050763
PRIMER_RIGHT_1_PENALTY=0.110392
PRIMER_LEFT_1_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_1_SEQUENCE=CGTCATCTCTTTTTCATCCACA
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.1836
PRIMER_LEFT_2_PENALTY=0.050763
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_2_SEQUENCE=GTGGCTTCCATTATCAACAACA
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=60.051
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.1836
PRIMER_LEFT_3_PENALTY=0.050763
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_LEFT_3_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_3_SEQUENCE=GTTGTGGCTTCCATTATCAACA
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=60.051
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.1836
PRIMER_LEFT_4_PENALTY=0.050763
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_4_SEQUENCE=TGTGGCTTCCATTATCAACAAC
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=60.051
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_00379 Ps_contig_mira-and-tgicl-ass_franssen_4068
SEQUENCE=GAACASAACACTAAATTCCACTCAACCCTACTTTCTCTCTCTTCTCTCTCTCTCTCTCTACAATCTCAACAATGGTCGTTCGCTTCTTTCCTCGGAAGAGTCCTACTTAAACGCCTCCGTCTTCATCCTCTCTGCTTA
TARGET=43,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00380 Ps_contig_mira-and-tgicl-ass_franssen_4091
SEQUENCE=CTTCTTTGCAGCCACCTGCAATGCATATTTACAGTTCAAAAATTGACAAAAAGCTCCTGGCCAGTTAGCAAACTCCAACCACAACATAAACATGGAAGCATTTTGAATTTATTTATTTCAAACCTCTTCTCCAATAATAGTAATGTTTGGATGAGTTTGTAGAGCACATTCCAGTGTAATGTGGGAAGCTGCACGCCCCATAAGCCGCACAAAATGGTAATATTTTCCGGTTGATCGGGCATCTATCATAACGTTTCCTATCATTTCTGCATATATCTTGCAAGCAGTATCAAACCCAAAGCTTGTCGGAACTTCTTTACTTTTCAGATCGCCATCAATGGTTTTGGGGCATCCAAGCACCTGTGTTTTTAAATTTTTGTCTTCTGAAGTTCTCGGCAAGGAGGCATGCATTCGTGTTGGAATCATCTCCACCAATAACAACAAGCCCATCCAACTCTAGCTTCTTCGCTGTTTCCTCAGCTTGTTTAAACTGCTCTGGAGTTTCAATCTTGTCCCTCCCACTGCAAATCATGTCAAAACCACCCTGATTTCTATAAGGATAAATATACTCAGAATTGAGTTCAGTGTATTTGCACTTCATAATGCCGGCAGGACCACCTTTGAAACCATACAATGTGCTTCCACTTGCAAGTTGTTGAAGATAATCGTAAATTCCAGATATAACATTGTGACCTCCAGGAGCCTGTCCACCGGAGAGAACAACGCCAATCTTCAACTTCCGATGAGGAAGCGCATTGGTCGAATCAGCAGGCACCAAAGCAGCGGAAGGTTGTCCAAACAAAAATGGAAACAGTTTCGCGATCTCATCTGGATTTCCGGCGGCGGAGCTTTTAGGACCGTCGACAATGGCGAACGGTGTTTGGAGAACGGAAGGAAGACGGAGTTTGTGATCGACGCGGCTGTTTTGGACTTCGCTGTAGACGGAAGCGAAGCGAGTTGTGACGGTGGGAGTTACGTTTCCGTTACTTACGATGGAAGCTGGTGCCATTGTTTGTTGAGAGAGTGAGAGAGAGAGTGTGTGTGTTGTTGAGAAGTCCCCCCGTACTCTGCGTTGATAC
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3686
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.273036
PRIMER_LEFT_SEQUENCE=GCAAACTCCAACCACAACATAA
PRIMER_RIGHT_SEQUENCE=AACGTTATGATAGATGCCCGAT
PRIMER_LEFT=67,22
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.727
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.3896
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.294013
PRIMER_LEFT_1_SEQUENCE=GCAAACTCCAACCACAACATAA
PRIMER_RIGHT_1_SEQUENCE=GGAAACGTTATGATAGATGCCC
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=59.706
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.4764
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.273036
PRIMER_LEFT_2_SEQUENCE=CAGTTAGCAAACTCCAACCACA
PRIMER_RIGHT_2_SEQUENCE=AACGTTATGATAGATGCCCGAT
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.727
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.4974
PRIMER_LEFT_3_PENALTY=0.203343
PRIMER_RIGHT_3_PENALTY=0.294013
PRIMER_LEFT_3_SEQUENCE=CAGTTAGCAAACTCCAACCACA
PRIMER_RIGHT_3_SEQUENCE=GGAAACGTTATGATAGATGCCC
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=59.706
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.5461
PRIMER_LEFT_4_PENALTY=0.513718
PRIMER_RIGHT_4_PENALTY=0.032347
PRIMER_LEFT_4_SEQUENCE=CCAACCACAACATAAACATGGA
PRIMER_RIGHT_4_SEQUENCE=TAAAAACACAGGTGCTTGGATG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=370,22
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=60.032
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_00381 Ps_contig_mira-and-tgicl-ass_franssen_4091
SEQUENCE=CTTCTTTGCAGCCACCTGCAATGCATATTTACAGTTCAAAAATTGACAAAAAGCTCCTGGCCAGTTAGCAAACTCCAACCACAACATAAACATGGAAGCATTTTGAATTTATTTATTTCAAACCTCTTCTCCAATAATAGTAATGTTTGGATGAGTTTGTAGAGCACATTCCAGTGTAATGTGGGAAGCTGCACGCCCCATAAGCCGCACAAAATGGTAATATTTTCCGGTTGATCGGGCATCTATCATAACGTTTCCTATCATTTCTGCATATATCTTGCAAGCAGTATCAAACCCAAAGCTTGTCGGAACTTCTTTACTTTTCAGATCGCCATCAATGGTTTTGGGGCATCCAAGCACCTGTGTTTTTAAATTTTTGTCTTCTGAAGTTCTCGGCAAGGAGGCATGCATTCGTGTTGGAATCATCTCCACCAATAACAACAAGCCCATCCAACTCTAGCTTCTTCGCTGTTTCCTCAGCTTGTTTAAACTGCTCTGGAGTTTCAATCTTGTCCCTCCCACTGCAAATCATGTCAAAACCACCCTGATTTCTATAAGGATAAATATACTCAGAATTGAGTTCAGTGTATTTGCACTTCATAATGCCGGCAGGACCACCTTTGAAACCATACAATGTGCTTCCACTTGCAAGTTGTTGAAGATAATCGTAAATTCCAGATATAACATTGTGACCTCCAGGAGCCTGTCCACCGGAGAGAACAACGCCAATCTTCAACTTCCGATGAGGAAGCGCATTGGTCGAATCAGCAGGCACCAAAGCAGCGGAAGGTTGTCCAAACAAAAATGGAAACAGTTTCGCGATCTCATCTGGATTTCCGGCGGCGGAGCTTTTAGGACCGTCGACAATGGCGAACGGTGTTTGGAGAACGGAAGGAAGACGGAGTTTGTGATCGACGCGGCTGTTTTGGACTTCGCTGTAGACGGAAGCGAAGCGAGTTGTGACGGTGGGAGTTACGTTTCCGTTACTTACGATGGAAGCTGGTGCCATTGTTTGTTGAGAGAGTGAGAGAGAGAGTGTGTGTGTTGTTGAGAAGTCCCCCCGTACTCTGCGTTGATAC
TARGET=1026,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7462
PRIMER_LEFT_PENALTY=0.269585
PRIMER_RIGHT_PENALTY=0.476630
PRIMER_LEFT_SEQUENCE=AAACAGTTTCGCGATCTCATCT
PRIMER_RIGHT_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT=808,22
PRIMER_RIGHT=1058,22
PRIMER_LEFT_TM=60.270
PRIMER_RIGHT_TM=59.523
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.9195
PRIMER_LEFT_1_PENALTY=0.442900
PRIMER_RIGHT_1_PENALTY=0.476630
PRIMER_LEFT_1_SEQUENCE=CTGTTTTGGACTTCGCTGTAGA
PRIMER_RIGHT_1_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT_1=920,22
PRIMER_RIGHT_1=1058,22
PRIMER_LEFT_1_TM=59.557
PRIMER_RIGHT_1_TM=59.523
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=1.0522
PRIMER_LEFT_2_PENALTY=0.575571
PRIMER_RIGHT_2_PENALTY=0.476630
PRIMER_LEFT_2_SEQUENCE=TGTTTTGGACTTCGCTGTAGAC
PRIMER_RIGHT_2_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT_2=921,22
PRIMER_RIGHT_2=1058,22
PRIMER_LEFT_2_TM=59.424
PRIMER_RIGHT_2_TM=59.523
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=1.5937
PRIMER_LEFT_3_PENALTY=0.269585
PRIMER_RIGHT_3_PENALTY=1.324098
PRIMER_LEFT_3_SEQUENCE=AAACAGTTTCGCGATCTCATCT
PRIMER_RIGHT_3_SEQUENCE=GGACTTCTCAACAACACACACA
PRIMER_LEFT_3=808,22
PRIMER_RIGHT_3=1057,22
PRIMER_LEFT_3_TM=60.270
PRIMER_RIGHT_3_TM=58.676
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=1.6643
PRIMER_LEFT_4_PENALTY=1.187697
PRIMER_RIGHT_4_PENALTY=0.476630
PRIMER_LEFT_4_SEQUENCE=GTTTTGGACTTCGCTGTAGACG
PRIMER_RIGHT_4_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT_4=922,22
PRIMER_RIGHT_4=1058,22
PRIMER_LEFT_4_TM=61.188
PRIMER_RIGHT_4_TM=59.523
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_00382 Ps_contig_mira-and-tgicl-ass_franssen_4091
SEQUENCE=CTTCTTTGCAGCCACCTGCAATGCATATTTACAGTTCAAAAATTGACAAAAAGCTCCTGGCCAGTTAGCAAACTCCAACCACAACATAAACATGGAAGCATTTTGAATTTATTTATTTCAAACCTCTTCTCCAATAATAGTAATGTTTGGATGAGTTTGTAGAGCACATTCCAGTGTAATGTGGGAAGCTGCACGCCCCATAAGCCGCACAAAATGGTAATATTTTCCGGTTGATCGGGCATCTATCATAACGTTTCCTATCATTTCTGCATATATCTTGCAAGCAGTATCAAACCCAAAGCTTGTCGGAACTTCTTTACTTTTCAGATCGCCATCAATGGTTTTGGGGCATCCAAGCACCTGTGTTTTTAAATTTTTGTCTTCTGAAGTTCTCGGCAAGGAGGCATGCATTCGTGTTGGAATCATCTCCACCAATAACAACAAGCCCATCCAACTCTAGCTTCTTCGCTGTTTCCTCAGCTTGTTTAAACTGCTCTGGAGTTTCAATCTTGTCCCTCCCACTGCAAATCATGTCAAAACCACCCTGATTTCTATAAGGATAAATATACTCAGAATTGAGTTCAGTGTATTTGCACTTCATAATGCCGGCAGGACCACCTTTGAAACCATACAATGTGCTTCCACTTGCAAGTTGTTGAAGATAATCGTAAATTCCAGATATAACATTGTGACCTCCAGGAGCCTGTCCACCGGAGAGAACAACGCCAATCTTCAACTTCCGATGAGGAAGCGCATTGGTCGAATCAGCAGGCACCAAAGCAGCGGAAGGTTGTCCAAACAAAAATGGAAACAGTTTCGCGATCTCATCTGGATTTCCGGCGGCGGAGCTTTTAGGACCGTCGACAATGGCGAACGGTGTTTGGAGAACGGAAGGAAGACGGAGTTTGTGATCGACGCGGCTGTTTTGGACTTCGCTGTAGACGGAAGCGAAGCGAGTTGTGACGGTGGGAGTTACGTTTCCGTTACTTACGATGGAAGCTGGTGCCATTGTTTGTTGAGAGAGTGAGAGAGAGAGTGTGTGTGTTGTTGAGAAGTCCCCCCGTACTCTGCGTTGATAC
TARGET=1036,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0691
PRIMER_LEFT_PENALTY=0.269585
PRIMER_RIGHT_PENALTY=2.799545
PRIMER_LEFT_SEQUENCE=AAACAGTTTCGCGATCTCATCT
PRIMER_RIGHT_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT=808,22
PRIMER_RIGHT=1078,21
PRIMER_LEFT_TM=60.270
PRIMER_RIGHT_TM=61.800
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=57.143
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=3.0880
PRIMER_LEFT_1_PENALTY=0.288468
PRIMER_RIGHT_1_PENALTY=2.799545
PRIMER_LEFT_1_SEQUENCE=ACGTTTCCGTTACTTACGATGG
PRIMER_RIGHT_1_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_1=974,22
PRIMER_RIGHT_1=1078,21
PRIMER_LEFT_1_TM=60.288
PRIMER_RIGHT_1_TM=61.800
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=3.1785
PRIMER_LEFT_2_PENALTY=0.378922
PRIMER_RIGHT_2_PENALTY=2.799545
PRIMER_LEFT_2_SEQUENCE=GAAGGAAGACGGAGTTTGTGAT
PRIMER_RIGHT_2_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_2=890,22
PRIMER_RIGHT_2=1078,21
PRIMER_LEFT_2_TM=59.621
PRIMER_RIGHT_2_TM=61.800
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=3.2424
PRIMER_LEFT_3_PENALTY=0.442900
PRIMER_RIGHT_3_PENALTY=2.799545
PRIMER_LEFT_3_SEQUENCE=CTGTTTTGGACTTCGCTGTAGA
PRIMER_RIGHT_3_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_3=920,22
PRIMER_RIGHT_3=1078,21
PRIMER_LEFT_3_TM=59.557
PRIMER_RIGHT_3_TM=61.800
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=3.2889
PRIMER_LEFT_4_PENALTY=0.489375
PRIMER_RIGHT_4_PENALTY=2.799545
PRIMER_LEFT_4_SEQUENCE=TTCCGTTACTTACGATGGAAGC
PRIMER_RIGHT_4_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_4=978,22
PRIMER_RIGHT_4=1078,21
PRIMER_LEFT_4_TM=60.489
PRIMER_RIGHT_4_TM=61.800
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00383 Ps_contig_mira-and-tgicl-ass_franssen_4098
SEQUENCE=ATTTTCGATGATGGCAGGTTTCTTCGTCTTCAGTGCTGTTCATCTTTACAACAAGTAGATCATCGCACGAAGTTAATGTAGTCTGTAACATGGGAGTTGTACTTATATTGAGATTGAAAATTTTGATTAGGCAATGATAAAAATCATAAGACAGGTTGTTTCTAACTTTATTATAGATGTTRCTTCTTTTTATTATTATTATTCTGTCATTTTAATTTTAGTATTTAGTTATGTGAGTATTAATCACTGTAGCAAACCAATTAACTGAAAATTGAAATGACATGAAAAACCACAC
TARGET=190,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00384 Ps_contig_mira-and-tgicl-ass_franssen_4107
SEQUENCE=AACGTAATGCAATACAAAATAACCGAAACTAATTTTTGTAGTTGTACACATGATTCAATAACTAAAATAGCATCAAACTATGGAACCATTCGTAATCAGTGTTTTCGGTTCGAGATATTACCGAATGCAGCACCGTTGATGAAGCGAATCTGGATCTTTCCGTTCCGAGTCTTAACAGAACCCTTACCTGCATAAACAACCAAACAAAAACCAGCAGCGAACCGATCAAAGAAAACCCTCTAACTTGAAATTGTCCAAAACAAAATTGAAGCAAGTGGCATCGTCCTTGTTTGATATCCTATGGCACCACTGAGTTGTTTTCGGTTTGGTGGAAGAAGATCAACGGCGAATGCGTGAGGTCATGGTTTTGACGGTCGTTTGCGTGTCGTTGTCCCAGATGACGGTGACGGACCCGTGTTGTGATGTTGTGAACAGTATTGATGATGGAAGGTTGAGCTATCACCGACAAGATTCTGCCACGGTGAGTGGTCGGATGTGCGGTGAATAGGACGGCGGTTGTGAAAGGATTGGAAAAGAAACGGCGGTCGTTTGAGGTTGTGGTTTTGGGATTCGTCGGAGGGTTAACGGTGAAGAGGGTGAGTTTAAGGGTTTGACGGCAAAGGGTGGTTTAGCGGCGCATGTGGAGATTTTGTGGAGGTGGGTCGGAGGTGCTTTGATGGTGAGAGAGAATTTGGAGAAAGATAAAGAAGATTTTCTGAGATAGAGAGATGAATCCTGGAAAAAGTTTTTGTTTGTTGGTTAATAGAGAGAGTCTGAGTGGTGAGAGAGAGATAGTGAATGAATTTGTTGAGAGTGGTGTGTGTAATGAGTGGTCTCGTCGGAGAGTACCCCGA
TARGET=783,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3507
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.317612
PRIMER_LEFT_SEQUENCE=TAACGGTGAAGAGGGTGAGTTT
PRIMER_RIGHT_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT=582,22
PRIMER_RIGHT=848,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.318
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.3507
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.317612
PRIMER_LEFT_1_SEQUENCE=AACGGTGAAGAGGGTGAGTTTA
PRIMER_RIGHT_1_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_1=583,22
PRIMER_RIGHT_1=848,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.318
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.3507
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.317612
PRIMER_LEFT_2_SEQUENCE=TTAACGGTGAAGAGGGTGAGTT
PRIMER_RIGHT_2_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_2=581,22
PRIMER_RIGHT_2=848,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=60.318
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.4750
PRIMER_LEFT_3_PENALTY=0.157415
PRIMER_RIGHT_3_PENALTY=0.317612
PRIMER_LEFT_3_SEQUENCE=CGGTGAAGAGGGTGAGTTTAAG
PRIMER_RIGHT_3_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_3=585,22
PRIMER_RIGHT_3=848,22
PRIMER_LEFT_3_TM=60.157
PRIMER_RIGHT_3_TM=60.318
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.6472
PRIMER_LEFT_4_PENALTY=0.329550
PRIMER_RIGHT_4_PENALTY=0.317612
PRIMER_LEFT_4_SEQUENCE=TGATGGTGAGAGAGAATTTGGA
PRIMER_RIGHT_4_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_4=674,22
PRIMER_RIGHT_4=848,22
PRIMER_LEFT_4_TM=59.670
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=59.091
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_00385 Ps_contig_mira-and-tgicl-ass_franssen_4120
SEQUENCE=TTGAAATTCCTATCACATTTCACCCTAATCTAATCTAATCTAATCTGTAATCGACGATTTACATAACATATGGACAACTTTGAGACAACAAATCATAATCTTGTTCATTACAGTATAATTTTGACATGTTATTGCTAGTTACAAATCCTTTTTTGTTATGCTACTTGTAATTTTGTCACTTTCATGAAATTCTATCAGTACTTACAGGGAAATTCAACCCATTCCCTCTTCCAAAG
TARGET=25,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00386 Ps_contig_mira-and-tgicl-ass_franssen_4133
SEQUENCE=CGTTTTCCACCCTTACGTAGAAATTTCCTTTTTCTTGTCTACAGGTGCCAGTCCATCTCTCACAGCAGCCGTTGTTGTGTAACTGGTCTATTGGGGCGGAATTTCGTGCAAGCAACTCTTGATAGACTCAGATTTAGTGATCCGATTGCCCGAGGAATAGCAACAGCTTCTAGTGCACATGGACTTGGAGCAGCAGCATTATCTGCAAAGGAACCTGAGGCTCTCCCATTTTGTGCCATTGCTTATGCTTTGAATGGCATATTTGGATCTTTACTATGTTCAGTTCCTATTGTCAGACAAAGCTTGCTTGCAATTATTGGCTGAAATTGTCATTAATATCACAATCTGTTACCATTTTCCTTCCTTCCTATGTCATAACTTCCATTGTTTTTATTACGGAAGTACTAGTACAAATGTATGTATAGAGAAATATATTGAGAATCATAATTTTGTGAGTTGTATAGGATGCAATAATCTATTCATTTGAGTAATCAAATATCTATGTCATATTCAATTTTTTTTCGTT
TARGET=357,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00387 Ps_contig_mira-and-tgicl-ass_franssen_4135
SEQUENCE=GGGGGGACTCACTCACCACCACCATGGACCCAACTTCCTTCGATCTCCCTCCACCACCACCCACCACCACCACCACCGTCCCCTTCTCCCTGGAACCCCCTTGTTTGGAACCATCATCATCCACTCCTTACCATTGATGTATGGGAGCATGCTTACTATCTTGACTATCAGAACCGGCGCCCTGATTATATATCAGTGTTCATGGATAAGCTTGTGTCTTGGGAAGCAGTCAG
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0782
PRIMER_LEFT_PENALTY=1.066501
PRIMER_RIGHT_PENALTY=0.011696
PRIMER_LEFT_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_SEQUENCE=GTAAGCATGCTCCCATACATCA
PRIMER_LEFT=26,21
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=1.2089
PRIMER_LEFT_1_PENALTY=1.197222
PRIMER_RIGHT_1_PENALTY=0.011696
PRIMER_LEFT_1_SEQUENCE=CAACTTCCTTCGATCTCCCTC
PRIMER_RIGHT_1_SEQUENCE=GTAAGCATGCTCCCATACATCA
PRIMER_LEFT_1=30,21
PRIMER_RIGHT_1=155,22
PRIMER_LEFT_1_TM=60.197
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=1.2531
PRIMER_LEFT_2_PENALTY=1.066501
PRIMER_RIGHT_2_PENALTY=0.186600
PRIMER_LEFT_2_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_2_SEQUENCE=TGGTAAGGAGTGGATGATGATG
PRIMER_LEFT_2=26,21
PRIMER_RIGHT_2=132,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=1.4391
PRIMER_LEFT_3_PENALTY=1.066501
PRIMER_RIGHT_3_PENALTY=0.372557
PRIMER_LEFT_3_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_3_SEQUENCE=CCCAAGACACAAGCTTATCCAT
PRIMER_LEFT_3=26,21
PRIMER_RIGHT_3=222,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=60.373
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=1.5429
PRIMER_LEFT_4_PENALTY=1.066501
PRIMER_RIGHT_4_PENALTY=0.476376
PRIMER_LEFT_4_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_4_SEQUENCE=TAAGCATGCTCCCATACATCAA
PRIMER_LEFT_4=26,21
PRIMER_RIGHT_4=154,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=60.476
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_00388 Ps_contig_mira-and-tgicl-ass_franssen_4150
SEQUENCE=CATTTTTTTCCCAATTCTAATTTCATATTACAACCTAAATTTTACCCTTAACAACCCATTAATTAACAAGATCTTACATAAAAACCTCTCAACCACACTACTCCAGACTTTTTTAATTAACATTTCCGCCCCGAGTTTTACCCCATTTTTTAATTCAACCCTTCCGTTTAAAAAAATCACGGTTGAGTTAGAATTTGAAAAAATGGGATAGGGACGTAACGGGTAATAAGAAGATTCTAGATTAAGGAGAGAAAGAGAGAGAGAGGGAGAGAGAGAGAGAGAACAAAAAGGAAGAGAAGATAGAAAGTTAGACGTGGATATGGATATCGACCGTACGGTAAACCCGG
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0456
PRIMER_LEFT_PENALTY=0.321492
PRIMER_RIGHT_PENALTY=2.724156
PRIMER_LEFT_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT=205,22
PRIMER_RIGHT=329,24
PRIMER_LEFT_TM=60.321
PRIMER_RIGHT_TM=59.276
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=3.2172
PRIMER_LEFT_1_PENALTY=0.321492
PRIMER_RIGHT_1_PENALTY=2.895724
PRIMER_LEFT_1_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_1_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=329,22
PRIMER_LEFT_1_TM=60.321
PRIMER_RIGHT_1_TM=57.104
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=3.4344
PRIMER_LEFT_2_PENALTY=0.710267
PRIMER_RIGHT_2_PENALTY=2.724156
PRIMER_LEFT_2_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_2_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT_2=210,22
PRIMER_RIGHT_2=329,24
PRIMER_LEFT_2_TM=59.290
PRIMER_RIGHT_2_TM=59.276
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=3.4481
PRIMER_LEFT_3_PENALTY=0.321492
PRIMER_RIGHT_3_PENALTY=3.126610
PRIMER_LEFT_3_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_3_SEQUENCE=TCGATATCCATATCCACGTCTAACT
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=329,25
PRIMER_LEFT_3_TM=60.321
PRIMER_RIGHT_3_TM=60.127
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=3.6060
PRIMER_LEFT_4_PENALTY=0.710267
PRIMER_RIGHT_4_PENALTY=2.895724
PRIMER_LEFT_4_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_4_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_4=210,22
PRIMER_RIGHT_4=329,22
PRIMER_LEFT_4_TM=59.290
PRIMER_RIGHT_4_TM=57.104
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_00389 Ps_contig_mira-and-tgicl-ass_franssen_4150
SEQUENCE=CATTTTTTTCCCAATTCTAATTTCATATTACAACCTAAATTTTACCCTTAACAACCCATTAATTAACAAGATCTTACATAAAAACCTCTCAACCACACTACTCCAGACTTTTTTAATTAACATTTCCGCCCCGAGTTTTACCCCATTTTTTAATTCAACCCTTCCGTTTAAAAAAATCACGGTTGAGTTAGAATTTGAAAAAATGGGATAGGGACGTAACGGGTAATAAGAAGATTCTAGATTAAGGAGAGAAAGAGAGAGAGAGGGAGAGAGAGAGAGAGAACAAAAAGGAAGAGAAGATAGAAAGTTAGACGTGGATATGGATATCGACCGTACGGTAAACCCGG
TARGET=267,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0456
PRIMER_LEFT_PENALTY=0.321492
PRIMER_RIGHT_PENALTY=2.724156
PRIMER_LEFT_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT=205,22
PRIMER_RIGHT=329,24
PRIMER_LEFT_TM=60.321
PRIMER_RIGHT_TM=59.276
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=3.2172
PRIMER_LEFT_1_PENALTY=0.321492
PRIMER_RIGHT_1_PENALTY=2.895724
PRIMER_LEFT_1_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_1_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=329,22
PRIMER_LEFT_1_TM=60.321
PRIMER_RIGHT_1_TM=57.104
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=3.4344
PRIMER_LEFT_2_PENALTY=0.710267
PRIMER_RIGHT_2_PENALTY=2.724156
PRIMER_LEFT_2_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_2_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT_2=210,22
PRIMER_RIGHT_2=329,24
PRIMER_LEFT_2_TM=59.290
PRIMER_RIGHT_2_TM=59.276
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=3.4481
PRIMER_LEFT_3_PENALTY=0.321492
PRIMER_RIGHT_3_PENALTY=3.126610
PRIMER_LEFT_3_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_3_SEQUENCE=TCGATATCCATATCCACGTCTAACT
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=329,25
PRIMER_LEFT_3_TM=60.321
PRIMER_RIGHT_3_TM=60.127
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=3.6060
PRIMER_LEFT_4_PENALTY=0.710267
PRIMER_RIGHT_4_PENALTY=2.895724
PRIMER_LEFT_4_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_4_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_4=210,22
PRIMER_RIGHT_4=329,22
PRIMER_LEFT_4_TM=59.290
PRIMER_RIGHT_4_TM=57.104
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_00390 Ps_contig_mira-and-tgicl-ass_franssen_4151
SEQUENCE=CTTTTAACCTAACCCTTTAAACCCGTTTTAAAAAACTTAACTACGGTTAGGTAGTTAAGTAATTAAGTAAAAGTAGGAGTAGGGACGGTAACGGTAAGTAAGAAGATNTANATAGAGAGAAGAGAGAGAGRRGAGAGAGAGAGAGACAAARAGGAAGAGAAGATGAAAAGTTGACGTGGATATGGATATGACCTGTAACCCGGAAATGACTCGTTTCAGACTCATGCAATACGAAGAAGACGTCTCAGATCCCGTGTTGCGGAGTCGTCGAAGGCCACCGGCGCAAACTTCCTCTGAAAAGACGGAATCGGAAGTGCGTTCGGCGACGGAGACATCGCGAACGCGGATCCGGCGTAAACTTCGCAACCAGTCTTGAAATCAACGATCCGGATCTGTTTAGGAACCATATTCGGGTCGGGTACGAGTCTCTGTGTTCCAATGACAACCAAATCGTCACCTTCCTTCTTCAAACCCTCGTTGTTCATCTTCGAATCAAGAGATTCGCCTCTCCTGAGAATCATCACCTTCTCCATCATCATCTCACTACCTTTTGCCATCTTCAAATCATCGTAGCTAGGTCTTCTCTCCGTTGTCATTTTCAAATCATCATAACTAGGTCTTTTCTCCGCCACCGCAACACGTTTTCTCTGTTCCGGTCGAATAACCGGTCTCCGGTTAGTGTTAACCCTCCCGGAGTTTGTTGCCCCATAAAAAGTATTACTATTCCCATTACCATAATTGTTATAATTATTATGGCAATTTGTATAAGTTCTTCGTCGGGAAAAACTTGTCGGAGGAACTCTGATTCGTTCATGCAAACAATCATGTGGTAGCAAAATTTCTGTTCCCATGGTGAATGGTAAAAAAATTCAAAAACTGAGAGAGGAAATGAAGAAGAAAAAAAGTATATGAGCGTTTAGATCTGAATAATTAATCAAAACGACAAAACTATTGTCGTTTTGTTAATTTTATGTTTAGAATTTCTATTCTATTGTAATAATATTATATTCTTCTCTGTTTTGTTTCTTCTTCTTCCTCTGAGAAGACAAGGAAGAGAAGAAGGGGGTGTTGATAAAAAAAAATTAGGAAGAAACAAGAAGAAAAGAAATGAAGAAAATATGAATTTTCGSGAACCCCC
TARGET=121,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00391 Ps_contig_mira-and-tgicl-ass_franssen_4151
SEQUENCE=CTTTTAACCTAACCCTTTAAACCCGTTTTAAAAAACTTAACTACGGTTAGGTAGTTAAGTAATTAAGTAAAAGTAGGAGTAGGGACGGTAACGGTAAGTAAGAAGATNTANATAGAGAGAAGAGAGAGAGRRGAGAGAGAGAGAGACAAARAGGAAGAGAAGATGAAAAGTTGACGTGGATATGGATATGACCTGTAACCCGGAAATGACTCGTTTCAGACTCATGCAATACGAAGAAGACGTCTCAGATCCCGTGTTGCGGAGTCGTCGAAGGCCACCGGCGCAAACTTCCTCTGAAAAGACGGAATCGGAAGTGCGTTCGGCGACGGAGACATCGCGAACGCGGATCCGGCGTAAACTTCGCAACCAGTCTTGAAATCAACGATCCGGATCTGTTTAGGAACCATATTCGGGTCGGGTACGAGTCTCTGTGTTCCAATGACAACCAAATCGTCACCTTCCTTCTTCAAACCCTCGTTGTTCATCTTCGAATCAAGAGATTCGCCTCTCCTGAGAATCATCACCTTCTCCATCATCATCTCACTACCTTTTGCCATCTTCAAATCATCGTAGCTAGGTCTTCTCTCCGTTGTCATTTTCAAATCATCATAACTAGGTCTTTTCTCCGCCACCGCAACACGTTTTCTCTGTTCCGGTCGAATAACCGGTCTCCGGTTAGTGTTAACCCTCCCGGAGTTTGTTGCCCCATAAAAAGTATTACTATTCCCATTACCATAATTGTTATAATTATTATGGCAATTTGTATAAGTTCTTCGTCGGGAAAAACTTGTCGGAGGAACTCTGATTCGTTCATGCAAACAATCATGTGGTAGCAAAATTTCTGTTCCCATGGTGAATGGTAAAAAAATTCAAAAACTGAGAGAGGAAATGAAGAAGAAAAAAAGTATATGAGCGTTTAGATCTGAATAATTAATCAAAACGACAAAACTATTGTCGTTTTGTTAATTTTATGTTTAGAATTTCTATTCTATTGTAATAATATTATATTCTTCTCTGTTTTGTTTCTTCTTCTTCCTCTGAGAAGACAAGGAAGAGAAGAAGGGGGTGTTGATAAAAAAAAATTAGGAAGAAACAAGAAGAAAAGAAATGAAGAAAATATGAATTTTCGSGAACCCCC
TARGET=133,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00392 Ps_contig_mira-and-tgicl-ass_franssen_4151
SEQUENCE=CTTTTAACCTAACCCTTTAAACCCGTTTTAAAAAACTTAACTACGGTTAGGTAGTTAAGTAATTAAGTAAAAGTAGGAGTAGGGACGGTAACGGTAAGTAAGAAGATNTANATAGAGAGAAGAGAGAGAGRRGAGAGAGAGAGAGACAAARAGGAAGAGAAGATGAAAAGTTGACGTGGATATGGATATGACCTGTAACCCGGAAATGACTCGTTTCAGACTCATGCAATACGAAGAAGACGTCTCAGATCCCGTGTTGCGGAGTCGTCGAAGGCCACCGGCGCAAACTTCCTCTGAAAAGACGGAATCGGAAGTGCGTTCGGCGACGGAGACATCGCGAACGCGGATCCGGCGTAAACTTCGCAACCAGTCTTGAAATCAACGATCCGGATCTGTTTAGGAACCATATTCGGGTCGGGTACGAGTCTCTGTGTTCCAATGACAACCAAATCGTCACCTTCCTTCTTCAAACCCTCGTTGTTCATCTTCGAATCAAGAGATTCGCCTCTCCTGAGAATCATCACCTTCTCCATCATCATCTCACTACCTTTTGCCATCTTCAAATCATCGTAGCTAGGTCTTCTCTCCGTTGTCATTTTCAAATCATCATAACTAGGTCTTTTCTCCGCCACCGCAACACGTTTTCTCTGTTCCGGTCGAATAACCGGTCTCCGGTTAGTGTTAACCCTCCCGGAGTTTGTTGCCCCATAAAAAGTATTACTATTCCCATTACCATAATTGTTATAATTATTATGGCAATTTGTATAAGTTCTTCGTCGGGAAAAACTTGTCGGAGGAACTCTGATTCGTTCATGCAAACAATCATGTGGTAGCAAAATTTCTGTTCCCATGGTGAATGGTAAAAAAATTCAAAAACTGAGAGAGGAAATGAAGAAGAAAAAAAGTATATGAGCGTTTAGATCTGAATAATTAATCAAAACGACAAAACTATTGTCGTTTTGTTAATTTTATGTTTAGAATTTCTATTCTATTGTAATAATATTATATTCTTCTCTGTTTTGTTTCTTCTTCTTCCTCTGAGAAGACAAGGAAGAGAAGAAGGGGGTGTTGATAAAAAAAAATTAGGAAGAAACAAGAAGAAAAGAAATGAAGAAAATATGAATTTTCGSGAACCCCC
TARGET=1024,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00393 Ps_contig_mira-and-tgicl-ass_franssen_4153
SEQUENCE=TCATATCCATATCCACGTCAACTTTTCATCTTCTCTTCCTTTTTGTCTCTCTCTCTCTCTCCCTCTCTCTCTCTTTCTCTCTCTTGTTAACCAAAATGGTACTTTA
TARGET=46,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00394 Ps_contig_mira-and-tgicl-ass_franssen_4153
SEQUENCE=TCATATCCATATCCACGTCAACTTTTCATCTTCTCTTCCTTTTTGTCTCTCTCTCTCTCTCCCTCTCTCTCTCTTTCTCTCTCTTGTTAACCAAAATGGTACTTTA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00395 Ps_contig_mira-and-tgicl-ass_franssen_4171
SEQUENCE=AATTCATTCCTCCATTCCTATGTTGATTTCGTTTGTTCCTTTTGTTTTTTGTTTTTGTCTGYATTTCCTATTAATTGGAATTGGATTTTTTTTTTATATTTTTTTGGTGCTATTTAGTTACCAGATCTGGTTTTTAATTCTGGCAATTAGTATGTCTAAGTCTTGTTGAAGGGTTGTATTTTAATTTTAAATTTTAATGAGTATTGATAAATATATATATATTGAATTTAGGG
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00396 Ps_contig_mira-and-tgicl-ass_franssen_4179
SEQUENCE=CGGTCCTRMAATTAATAATAATAATTTATAGATAAATAYTATTCAAACATTAGGTTACAATTATTTGGTTGCTTTTTGCAGATGACCACTGTTAAAATGGAGCATGATGCGACTATATACCATACTATATTTGCTTCTCTTTCCCTCTCTCTCCAAGTAGTCCTTACGAAACCCAACATGCATTCATGAGATCTGTTACCTTAACGCAAGCTATTGTTA
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00397 Ps_contig_mira-and-tgicl-ass_franssen_4181
SEQUENCE=CTTACTTTGGAGTTTGTGGTCTTAACCTTCGTAAAGACTACATAATTATCTGACTTGTTAGATAATTGCAAAGAGCATGAGATCTGCTTCCTCAATTCAAAAGGGAACTGTAGCTCCTGAGGGTGTATCTGGAGGAGCTCGCTGGAACTCATGGCGGAGAGATCGGAGAAGAATCGGCGGAGACTAAGATCAGTGTTGATTTTCCGGTGAAGAAAAACGGTTGTGTTAGAGAGAGAGAGAGAGAGAGAGAGAGGGAGAAGAACTAATAACGTCCGCGTACT
TARGET=228,26
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3797
PRIMER_LEFT_PENALTY=0.193133
PRIMER_RIGHT_PENALTY=0.186529
PRIMER_LEFT_SEQUENCE=GAACTGTAGCTCCTGAGGGTGT
PRIMER_RIGHT_SEQUENCE=CGCGGACGTTATTAGTTCTTCT
PRIMER_LEFT=104,22
PRIMER_RIGHT=276,22
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.4076
PRIMER_LEFT_1_PENALTY=0.221115
PRIMER_RIGHT_1_PENALTY=0.186529
PRIMER_LEFT_1_SEQUENCE=GGCGGAGACTAAGATCAGTGTT
PRIMER_RIGHT_1_SEQUENCE=CGCGGACGTTATTAGTTCTTCT
PRIMER_LEFT_1=175,22
PRIMER_RIGHT_1=276,22
PRIMER_LEFT_1_TM=59.779
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=0.5005
PRIMER_LEFT_2_PENALTY=0.193133
PRIMER_RIGHT_2_PENALTY=0.307346
PRIMER_LEFT_2_SEQUENCE=GAACTGTAGCTCCTGAGGGTGT
PRIMER_RIGHT_2_SEQUENCE=ACGCGGACGTTATTAGTTCTTC
PRIMER_LEFT_2=104,22
PRIMER_RIGHT_2=277,22
PRIMER_LEFT_2_TM=59.807
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.5285
PRIMER_LEFT_3_PENALTY=0.221115
PRIMER_RIGHT_3_PENALTY=0.307346
PRIMER_LEFT_3_SEQUENCE=GGCGGAGACTAAGATCAGTGTT
PRIMER_RIGHT_3_SEQUENCE=ACGCGGACGTTATTAGTTCTTC
PRIMER_LEFT_3=175,22
PRIMER_RIGHT_3=277,22
PRIMER_LEFT_3_TM=59.779
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=0.6412
PRIMER_LEFT_4_PENALTY=0.454655
PRIMER_RIGHT_4_PENALTY=0.186529
PRIMER_LEFT_4_SEQUENCE=TCCTCAATTCAAAAGGGAACTG
PRIMER_RIGHT_4_SEQUENCE=CGCGGACGTTATTAGTTCTTCT
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=276,22
PRIMER_LEFT_4_TM=60.455
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_00398 Ps_contig_mira-and-tgicl-ass_franssen_4194
SEQUENCE=ACAAGGCGTTGGTAATCTTCAAATCATGTTTGGATCTATTGGTTTGAGGCCTGCTGTTGACACACGTTGGAGTTCACCGCACCGTTATTCTTTTCTTCATTGCGTTTCCGGTTAGTAATCCCTGAGCATTAGATTTTATTTTATTTTTTATATATATTGTTGAGGGTCGTGTTTGTTCTCAAATGAAAAAGCAGAGGGATGTTGCAAATCAAAGGGAGTGGATTTTAGCGTTTTTCTTTGTTATTGTTCTGGAACATCATAAGTCTTAATTAATTTTATTATTATTATTTCATCCCTATTAATCTATTTGGGATTTAGAAGTCATTTGGRCTGWATWTTTTTTACAACCTCAAGCAAACTCTAAATTTAMAWAWAMMMWGNTTGTTATGTACCACCCATTTTATTACTTTTATTGTCATTTTTTGTCTTTTCTTATTCAAAATTAAGGATCGAACCGTAAGGGTTGTATTAAGAGGACCTATTGGGTACC
TARGET=133,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00399 Ps_contig_mira-and-tgicl-ass_franssen_4194
SEQUENCE=ACAAGGCGTTGGTAATCTTCAAATCATGTTTGGATCTATTGGTTTGAGGCCTGCTGTTGACACACGTTGGAGTTCACCGCACCGTTATTCTTTTCTTCATTGCGTTTCCGGTTAGTAATCCCTGAGCATTAGATTTTATTTTATTTTTTATATATATTGTTGAGGGTCGTGTTTGTTCTCAAATGAAAAAGCAGAGGGATGTTGCAAATCAAAGGGAGTGGATTTTAGCGTTTTTCTTTGTTATTGTTCTGGAACATCATAAGTCTTAATTAATTTTATTATTATTATTTCATCCCTATTAATCTATTTGGGATTTAGAAGTCATTTGGRCTGWATWTTTTTTACAACCTCAAGCAAACTCTAAATTTAMAWAWAMMMWGNTTGTTATGTACCACCCATTTTATTACTTTTATTGTCATTTTTTGTCTTTTCTTATTCAAAATTAAGGATCGAACCGTAAGGGTTGTATTAAGAGGACCTATTGGGTACC
TARGET=276,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00400 Ps_contig_mira-and-tgicl-ass_franssen_4196
SEQUENCE=TATATAAAAAATAAAATAAAATCTAATGCTCAGGGATTACCAACAGCAAACACAATGATGAAAATAACGGTGCGGTGAACTCCGACGTGCGTCAACAGCAGGCATCAAACCAAATAGATCCAAACATGATTTGAAGATWACCAACGCCTTGTCAACACGGTGTTGTCGACATCATGAATCCATGGTTA
TARGET=8,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00401 Ps_contig_mira-and-tgicl-ass_franssen_4217
SEQUENCE=GCCTTAAAGCAATATAAATAAATAAAAGTATTAGATCATAGAATCATACYYWTTACATCCCTCACTTTTATTATTTATTTCCACCTTCTCTCATAGAAAGTGAGGAATATTTAATTTGCATGACTAWTTTAACTTAGGAAAGTCAAGTCACACTACTATTGCATCATATACAAATGACAACATAGAATAACCAAAATAAAATACGTTCAGAGTGCTCTTATTAGAGTCCAAT
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00402 Ps_contig_mira-and-tgicl-ass_franssen_4248
SEQUENCE=AAGCTAAGTCTAGATTGGGATTGTCTTATGCTAAAAGGGAAGATGGCAAGGAGTCTATCTTTGGTGATGAGTCAAGAGTAACTATTCAAGAAGTTGAAGTGTTTTGTAGCCCTAAAATTGCAAGCTTATATTAGAATTTCATGAAAAATACTATACTATACTAATTCTCCTACTTTGAAGCATTTGTTTTTCTTTTATCAGAAAGAGAGATTGTAAAACTTTAAAACTAAGTACTGCTTATTATTATACTATTACTTAAAGTTAATTAATAAGAAAAAAACCT
TARGET=148,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00403 Ps_contig_mira-and-tgicl-ass_franssen_4262
SEQUENCE=GCAGAGTACGTCGGGATCATCATCAGAAGAAAAAGATACTATGTGGTGAAACCGAGGGCCAAACTCCAACAACAACAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATCTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCATCTGAACCGTCCGNTTCAACTTACCCCAAAAAAACGTCCGGGGGTTGGGTTTTGGTCCTCTAATGTGAACACTAAAAGGAAGCAAACAACTAAAGGAAG
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2423
PRIMER_LEFT_PENALTY=1.021310
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_SEQUENCE=TCACATTAGAGGACCAAAACCC
PRIMER_LEFT=11,21
PRIMER_RIGHT=239,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=1.2653
PRIMER_LEFT_1_PENALTY=1.021310
PRIMER_RIGHT_1_PENALTY=0.243965
PRIMER_LEFT_1_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_1_SEQUENCE=GGTGGTGAAATAAGTTGCGAAT
PRIMER_LEFT_1=11,21
PRIMER_RIGHT_1=118,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=60.244
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=1.2703
PRIMER_LEFT_2_PENALTY=1.132848
PRIMER_RIGHT_2_PENALTY=0.137489
PRIMER_LEFT_2_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_2_SEQUENCE=ACGGTTCAGATGCTTTTCAGAT
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=61.133
PRIMER_RIGHT_2_TM=60.137
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=1.2802
PRIMER_LEFT_3_PENALTY=1.021310
PRIMER_RIGHT_3_PENALTY=0.258908
PRIMER_LEFT_3_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_3_SEQUENCE=CATTAGAGGACCAAAACCCAAC
PRIMER_LEFT_3=11,21
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=59.741
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=1.2802
PRIMER_LEFT_4_PENALTY=1.021310
PRIMER_RIGHT_4_PENALTY=0.258908
PRIMER_LEFT_4_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_4_SEQUENCE=ACATTAGAGGACCAAAACCCAA
PRIMER_LEFT_4=11,21
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=60.021
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_00404 Ps_contig_mira-and-tgicl-ass_franssen_4263
SEQUENCE=TCGGGGGKTGGTGAAACCGAGGGCCAAACTCCAACAACAACAAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATACTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCAATCTGAACCGTCCGATTCAATTCCCCAAAAAAACTACCGGGGTGGGTTTTGGTCCGTCTAATGTGAACACTAAAGGAAGCAAACACTAAAGGAAGCAGAAGGGACAAGAGAAGAGCTTCGATAA
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00405 Ps_contig_mira-and-tgicl-ass_franssen_4264
SEQUENCE=GTCGGGGATCATTATCATCATCAGAAGAAAAAAGATACTATGTGGTGAAACCGAGGGCCAAACTCCAACAAACAAACAAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATCTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCAATCTGAACCGTCCGATTCAATTCCAAAAATCCGGGTGGTTTGGTCCGTCTAATGTGAACACTAAAAGGAAGCAAAAA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2350
PRIMER_LEFT_PENALTY=1.132848
PRIMER_RIGHT_PENALTY=0.102129
PRIMER_LEFT_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_SEQUENCE=GTGTTCACATTAGACGGACCAA
PRIMER_LEFT=35,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=61.133
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=1.3264
PRIMER_LEFT_1_PENALTY=1.224301
PRIMER_RIGHT_1_PENALTY=0.102129
PRIMER_LEFT_1_SEQUENCE=GATACTATGTGGTGAAACCGAGG
PRIMER_RIGHT_1_SEQUENCE=GTGTTCACATTAGACGGACCAA
PRIMER_LEFT_1=33,23
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=59.776
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=1.5283
PRIMER_LEFT_2_PENALTY=1.132848
PRIMER_RIGHT_2_PENALTY=0.395499
PRIMER_LEFT_2_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_2_SEQUENCE=AACTGTGAGGATGGGAGAGAAC
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=159,22
PRIMER_LEFT_2_TM=61.133
PRIMER_RIGHT_2_TM=59.605
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=1.5317
PRIMER_LEFT_3_PENALTY=1.132848
PRIMER_RIGHT_3_PENALTY=0.398877
PRIMER_LEFT_3_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_3_SEQUENCE=TGTTCACATTAGACGGACCAAA
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=233,22
PRIMER_LEFT_3_TM=61.133
PRIMER_RIGHT_3_TM=60.399
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=1.6049
PRIMER_LEFT_4_PENALTY=1.132848
PRIMER_RIGHT_4_PENALTY=0.472052
PRIMER_LEFT_4_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_4_SEQUENCE=TGGGAGAGAACGAATGATGATA
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=148,22
PRIMER_LEFT_4_TM=61.133
PRIMER_RIGHT_4_TM=59.528
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_00406 Ps_contig_mira-and-tgicl-ass_franssen_4266
SEQUENCE=AAGTTACACAGCTAATTTTAAGAAGGTAAAGGGTATTAAATGCAGCTCAGTACATAATATATATATACAAAAAGAAGTTTCATAGCTAAGAAAAGAAGTGA
TARGET=57,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00407 Ps_contig_mira-and-tgicl-ass_franssen_4267
SEQUENCE=TCGGGTACSACTTCATTCATTCATTCATTCTCCGATCTTCACCGCTCCGGTTCTTTGATTCCGGTTCACGTAAAAAACCGAAAAAGAATAACCGAACCGTAAAATCTCGGTGCTCTAATGGAAGCTCACGGCGGAGGACTCCGCCGCGTCCTGGTACTAGCCTTCTGCGTCGCCGGAATCTGGTCGGCTTATATCTACCAAGGCATTCTTCAGGAAACACTGTCAACGAAACGATTCGGTCCAGACGGTGAGAGATTTGAACACCTAGCGGTTTTGAACTTGGCACAGAACGTAGTGTGTTTAATCTGGTCATTCATAATGATACAGATGTGGTCTGGTGGAATTTCTGGTGGTGCTCCTTGGTGGTCTTATTGGAGTGCTGGTATTACTAACACCATTGGTCCTGCTATGGGAATTGAAGCTTTGAAGTATATTAGTTACCCTGCTCAGGTGCTGGCAAAGTCCTCAAAAATGATTCCAGTTATGCTGATGGGTTCACTCGTATACGGTATAAGATACACCATTCCAGAATACCTTTGCACCTTCCTTGTTGCTGGAGGGGTTTCAA
TARGET=12,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00408 Ps_contig_mira-and-tgicl-ass_franssen_4268
SEQUENCE=GGGGATCACACCATTGAGTGGAGAGTGGTATATCGAACAACATTAGAATTAAATATTAAGAAAAAAAAGAAAGAGTGTAAGGTTGTGAGGTACAGAGGAGATAGAAAAAGGAAGTGAGAACAGAGAGAGTGAAGWMTGAAAATAGATAGATAGATCTGAAGAAAAAAATGTCGACGGGTTAGTAGATCTGTATTCGAAGGGGTATTTAGGTTAAGAGTTGGGTTGTTTTTTGGG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00409 Ps_contig_mira-and-tgicl-ass_franssen_4282
SEQUENCE=CTTAAGTATTCAAGTGTTTTCGGTTTGAATTTCGAAACAACAACAACTGGGTGGAGTGGGTGGGTTGGAAGGGAAAAATTAGGGGAGGGAAAAAAGGGTAGCATAGATAGTGTTTGTGATATGGCTTCTGCTAGCCTTGGAAGTGGAAATGGAAATGGTGGTGTTGGTAGTTCTAGGTCTGCTTTAAGAGGTTCTTCAAGTTCTGTTGATTGGCTTGGGAGAGAGATGCTTGAGATGAGGCTCAGAGATAAAGTTGAGCATGACCATGATGACGATGATGATGGCAGGGAGAGTGAGCCTGATATCATTGATGGTGTGGGTGCTGAAACAGGACATGTAATACGAACCAGCATTGGTGGACGAAATGGTCAATCTAAGCAGAATGTTAGTTATATTGCGGAACACGTAGTTGGGACGGGCTCTTTTGGCACTGTTTTTCAGGCAAAATGTAGAGAAACAGGAGAGATTGTTGCCATCA
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00410 Ps_contig_mira-and-tgicl-ass_franssen_4302
SEQUENCE=TACCGTTTTTGTTTTTATTATTAATGTAATACAACAATTAACGCACAAATAAAATAATACAAGATTGTATTTTGTATTAGAGAGGAAAGATCAGTATCAAGAAAAGAAGAGACGACATTATATATACTTTTGAATCATTTCTCTGACTTCTTCAATAACTAGCCATGCCTTATGACCTCCAATCACTTCTGCTTTCATTTCTCCATCTTTCCAAAGCTGAATTGTTGGCATTTTAGAAATGTTGCCACGCTTGACTAGAGACTGAGGTACTTTATTAACATCCACATAGTAAATTTGTTCTGCAAAGTAAGAAAATAAATAATTAGTAGAAAGAGTAATTGAATCAAATTAATTAATTAAGTAAAATGTAATGAAAGGAGTTAGTTTTACTTGGTGTACTAAATTCAGCTGTCTTTAA
TARGET=347,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.8973
PRIMER_LEFT_PENALTY=0.139083
PRIMER_RIGHT_PENALTY=4.758221
PRIMER_LEFT_SEQUENCE=ATGACCTCCAATCACTTCTGCT
PRIMER_RIGHT_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT=171,22
PRIMER_RIGHT=412,24
PRIMER_LEFT_TM=60.139
PRIMER_RIGHT_TM=57.242
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=4.9509
PRIMER_LEFT_1_PENALTY=0.139083
PRIMER_RIGHT_1_PENALTY=4.811858
PRIMER_LEFT_1_SEQUENCE=ATGACCTCCAATCACTTCTGCT
PRIMER_RIGHT_1_SEQUENCE=AGACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_1=171,22
PRIMER_RIGHT_1=413,25
PRIMER_LEFT_1_TM=60.139
PRIMER_RIGHT_1_TM=58.188
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=4.9734
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=4.758221
PRIMER_LEFT_2_SEQUENCE=ATGCCTTATGACCTCCAATCAC
PRIMER_RIGHT_2_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=412,24
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=57.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=5.0013
PRIMER_LEFT_3_PENALTY=0.243031
PRIMER_RIGHT_3_PENALTY=4.758221
PRIMER_LEFT_3_SEQUENCE=GACCTCCAATCACTTCTGCTTT
PRIMER_RIGHT_3_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_3=173,22
PRIMER_RIGHT_3=412,24
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=57.242
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=5.0013
PRIMER_LEFT_4_PENALTY=0.243031
PRIMER_RIGHT_4_PENALTY=4.758221
PRIMER_LEFT_4_SEQUENCE=ACCTCCAATCACTTCTGCTTTC
PRIMER_RIGHT_4_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_4=174,22
PRIMER_RIGHT_4=412,24
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=57.242
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_00411 Ps_contig_mira-and-tgicl-ass_franssen_4321
SEQUENCE=GAAGAGAAGATGTTCCATTACWCGGCASKGGCCTACACCACTCCACGTTACCATCTTTGTCCGSAAAACCAACAATTCCATACCCATCTTCGTCCTCACAAAATTAAATATCTCCATCTACATACACTACACCTTTTCAATACTCAACTCTTCTCTCTCTCTCTCTTTTCTACCATGGTTGCAAGCTTGGGAAACCTAGCGTTATTACTCGACATTTCTTCGCCGCGAACCTTATCTCTCGATAGAAAGTCTCGTCTCGTTGGCGTAGACGCAATTCTCACCTTACCGCTCAAGCGAGACTCTTCTTCTTCTTCTTCTACTTCCTCCTCTTGCGGATACGATGGGGAGGTACGTGGGCGCGTGGTGGTGGCGCGTGGGAAGTCAAACTCGGAGCAGAACGGGGTTGATTTTGACGCGGAGTGGAGTGACGAGGAAGAAGGAGAGTTTGGGGATGATTTTGAGAAGATAAGGATGAGAGTGAGGGAGCTTGAGGAGAGGAGAGTGAGGGAGCTTGAGGAGAGGAGAGAGCTTGAGAAGAAAACGGAAGAGTATTTGCAGAGTGAAAAAATCGATGATGGAGATGAAGAGATCACCGAGGAAGAGAAGAGGATGAGAGTCAGGAAAGTGCTCGAAAAGGTGGCTAAGGAGCAGGCAGAGAAGAGGGCCATGGCTCAGTTGATGTTCGATTTGGGTCAGAAAGCTTACGGGAGAGGCAAGTATGGACGGGCCATTAAATTTCTTGAAGGTGCGCTCACTATCATAGCAAGACCTACATTATTCGGTGGTGAGATCCAAATATGGTTGGCCATGGCTTATGAGGCCAATAATCGCCATAACGATTGCATTGATCTATACAAGCAATTAGAAAAGTCACACCCTATTGTTAGCATCCGGCGCCAAGCTGCAGAGCTTCGTTACATTTTGGAAGCACCAAAGCTAAAGATAACCCAGGAGGAGATGTTATGCTGTAACATGGAGTGATAAAAACAAGGGTAGAAAGAGTGGAACAGTTAACACTCCATTTCCATCATCAAAAGATAACCTTTTGGATTCTCTTGTGTGGAGCCCTCCTACTGGATTAGGGAAAAATCGAGCATTCTGGGTTGGTTTAACATTATGGTTGGGATTAGTAGGTGCTGCTCTCTTCATTCAAAGATGAAGTTCCAGTTCTTCAATTTCTGATGATTCTAATTTTGTGTTATAATTTTTCATTTTAGTTTTTAGTGTATATAGTCCATTGTAAAATTATTGTATGTAACCAATAGGAAATATGTGTTATTTTATTATATTTTCCTGTTAGGGTGGTTGTTCTATTGAAAGTACAAGATTGTAAGTTCAAATTTAATTGTGTCATTGTATATTGAAAAAATAATTGTTGAATTACAAATATTTATGGTAATTTCTGTTAAACTT
TARGET=152,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00412 Ps_contig_mira-and-tgicl-ass_franssen_4321
SEQUENCE=GAAGAGAAGATGTTCCATTACWCGGCASKGGCCTACACCACTCCACGTTACCATCTTTGTCCGSAAAACCAACAATTCCATACCCATCTTCGTCCTCACAAAATTAAATATCTCCATCTACATACACTACACCTTTTCAATACTCAACTCTTCTCTCTCTCTCTCTTTTCTACCATGGTTGCAAGCTTGGGAAACCTAGCGTTATTACTCGACATTTCTTCGCCGCGAACCTTATCTCTCGATAGAAAGTCTCGTCTCGTTGGCGTAGACGCAATTCTCACCTTACCGCTCAAGCGAGACTCTTCTTCTTCTTCTTCTACTTCCTCCTCTTGCGGATACGATGGGGAGGTACGTGGGCGCGTGGTGGTGGCGCGTGGGAAGTCAAACTCGGAGCAGAACGGGGTTGATTTTGACGCGGAGTGGAGTGACGAGGAAGAAGGAGAGTTTGGGGATGATTTTGAGAAGATAAGGATGAGAGTGAGGGAGCTTGAGGAGAGGAGAGTGAGGGAGCTTGAGGAGAGGAGAGAGCTTGAGAAGAAAACGGAAGAGTATTTGCAGAGTGAAAAAATCGATGATGGAGATGAAGAGATCACCGAGGAAGAGAAGAGGATGAGAGTCAGGAAAGTGCTCGAAAAGGTGGCTAAGGAGCAGGCAGAGAAGAGGGCCATGGCTCAGTTGATGTTCGATTTGGGTCAGAAAGCTTACGGGAGAGGCAAGTATGGACGGGCCATTAAATTTCTTGAAGGTGCGCTCACTATCATAGCAAGACCTACATTATTCGGTGGTGAGATCCAAATATGGTTGGCCATGGCTTATGAGGCCAATAATCGCCATAACGATTGCATTGATCTATACAAGCAATTAGAAAAGTCACACCCTATTGTTAGCATCCGGCGCCAAGCTGCAGAGCTTCGTTACATTTTGGAAGCACCAAAGCTAAAGATAACCCAGGAGGAGATGTTATGCTGTAACATGGAGTGATAAAAACAAGGGTAGAAAGAGTGGAACAGTTAACACTCCATTTCCATCATCAAAAGATAACCTTTTGGATTCTCTTGTGTGGAGCCCTCCTACTGGATTAGGGAAAAATCGAGCATTCTGGGTTGGTTTAACATTATGGTTGGGATTAGTAGGTGCTGCTCTCTTCATTCAAAGATGAAGTTCCAGTTCTTCAATTTCTGATGATTCTAATTTTGTGTTATAATTTTTCATTTTAGTTTTTAGTGTATATAGTCCATTGTAAAATTATTGTATGTAACCAATAGGAAATATGTGTTATTTTATTATATTTTCCTGTTAGGGTGGTTGTTCTATTGAAAGTACAAGATTGTAAGTTCAAATTTAATTGTGTCATTGTATATTGAAAAAATAATTGTTGAATTACAAATATTTATGGTAATTTCTGTTAAACTT
TARGET=301,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00413 Ps_contig_mira-and-tgicl-ass_franssen_4342
SEQUENCE=TATTTGAACCTTGAAGGACAAAGCAATGAAACTTGCATCTTGAAACCAATGAAGATTCATTTTTCTCTTGTAGATTTTCATAAATCAGAAGTATGACCATCCTAAGTCCTATTGTATATATTGAAAATCACGAAATTATTTTTCATGCTCATAAATAAACTATTCTTGAAAAGTATGATCACAATGAAATCAATTTATTTATTTACATGTACATAATATCTTTTTGGTCAATAGATTATTGGATTGAAATAWAATTGTCACAGGTTACTCTTGCTATTGATATTGTGTTTGAATGTATAATTAGGAACTTAGGATCGAATAAGTACAATCACTATTTCGATAGTATACAAGTAATAGTGTGATCACACTGCTCACTACTTTTATTATTCCTA
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00414 Ps_contig_mira-and-tgicl-ass_franssen_4357
SEQUENCE=CTTGGAGCAACATGTGTTTACCCATTACAGGTKGGGAGAACCTTGCGGGTGAATTTCTCGGACAAACCGAAGCCGAAAGAACCGTTGTACCCTGAATCCGAATACAAGCTTTTCGTTGGGAACTTGTCTTGGACAGTAACTTCTGAGAGTCTGGCAGAAGTCTTTCAACAACATGGAACTGTGGTTGGAGCTAGGGTGTTGTATGATGGCGAAACGGGAAAGTCTCGTGGCTATGGCTTTGTTTGCTATGCCAATAAATCAGAAATGGAAGCTGCCCTTACAGTCATGAATGATGTGGAACTAGATGGACGGACATTGCGACGKAAGTTTGGCGCANNAAACGGAAAAACGTACTACTAAAGTTAACGGGTACGTAAGTAAACGAAAACCAACGAAAAAGGAAAAACGTACCGTCCGACGAAGAGTTGGTTACGAAACTAAGTTGGTTGGTCGAACCGAAAAGAAAGTAGGAAGGAAGGAAGG
TARGET=469,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00415 Ps_contig_mira-and-tgicl-ass_franssen_4358
SEQUENCE=ATGGGTGGGGGTGGGCGGGTGGGTGGGTGGTGCTGCAAGAAAGAAACCACGGGTGGGTGGGAAGTAGAAAGAAAAATAGAATATTATTATAATCCACCAAGGTATGGTATGGTAGTTTTATGCTTATCCTGGACCAGTTTACCCTCCACAGTATTTTCAGTGATGACGAGAACCCCAATGCTTGTTCTGTAATGTAAAAATTATTCAAGAATGGAAATGGTAAGGGTAATATTGGAATTATGAAATTTAGACTCTATGAGTGAAATGTGTTTGTGATAGCCATATCCTGGGTAAGATGTGCCCTATGTACTTTTCCCTGCAGTAGAAAAGTGTAATCTACAGATAACTGTAGAGTTTTGAAATGCTTGTACTTTTTTTATTTAACCAAAATTAATTGTTATATATGAAAAATATATAAATAATTAAAGTGTTTTATCTWWAACT
TARGET=17,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00416 Ps_contig_mira-and-tgicl-ass_franssen_4359
SEQUENCE=TGGTATCAACGACAGAGAACGAGACACTATATTTCATACATAAAAAAACACCGCAAATAAAAGTAGCCGCAGAACCATCCTCGCTTATCTCTCTATCTCTCTCTAACCTTTCAGTTCAGTTACACTAAATCAATGGGAGAGGAAGAAAAGAAACCAGAGGAAACTAAAGTTGAGGAGAAAAAACCAGAAGAACCGAAGAAAGAAGAAGAGAAGAAAGAAGATGAAAAAGCCACAGAGGAGAAAAAGCCGGAGGAATCTAAGGAACCGGTTGCCCCGCCGGAAATTGTGCTTAAAGTTTTCATGCATTGTGAAGGCTGTGCTCGTAAAGTTCGTCGTTCACTCAAAGGATTTCCAGGTGTTGAAGATGTTGTAACTGATTGCAAATCTCACAAGGTTGTTGTGAAAGGAGAGAAAGCGGATCCATTGAAGGTTCTAGAAAGAGTACAAAGGAAGAGTCATAGGCAAGTTGAGCTTCTTTCTCCGATCCCGATACCACCGTCGGAAGAAGATAAAAAATCGGAAGAGAAAGAGAAACCTAAACAAGAGGAAGAGAAGAAAGAAGAGCCTCAAGTTATCACAGTGATTCTGAAAGTTCACATGCATTGTGAAGCTTGTTCACAGGAAATCAAAAGACGCATTGAGAGAATCAAAGGAGTGGAATCAGCAGAACCGGATCTGAAGAACTCACTAGTGACAGTGAAAGGAGTGTTTGAAAGTGAGAAATTAGTTGAATATGTTTACAAGAGAACCGGAAAACAAGCGGTGATAGTGAAACAAGAGGCGGAGAAGAAAGAAGAAGCGAAAGAAACAAAGGAAGAAGAGAAGAAAACTGAGGAGAAGGACAAGAATGAGAAAGGAGGTGGTGAAGGAGAGGAAAAGAAAGAAGCTGGAGAAGGAGATGAAAAATCAGATGGTGGGGGTGGCGGTGGTGGTGGTGCTGCAGAAGAAACCACGGTGGTGGAAGTGAAGAAAAATGAATATTATTATAATCCACCAAGGTATGGTATGGAGTTTTATGCTTATCCTGGACCAGTTTACCCTCCACAGATTTTCAGTGATGAGAACCCCAATGCTTGTTCTGTAATGTAAAAAATTATTCAAGAATGGAAATGGTAAGGGTAATATTGGAATTATGAAATTTAGACTCTATGAGTGAAATGTGTTTGTGATAGCCATATCCTGGGTAAGATGTGCCCTATGTACTTTTCCCTGCAGTAGAAAAGTGTAATCTACAGATAACTGTAGAGTTTTGAAATGCTTGTACTTTTTTTATTTAACCAAAATTAATTGTTATATATGAAAAAATATATAAATAATTAAAGTGTTTTATACTTY
TARGET=925,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00417 Ps_contig_mira-and-tgicl-ass_franssen_4371
SEQUENCE=GAAAGTGAAGAGAGGAGGTGAACATTACGAAGTAACAACAACAACACTTATTTCGTCAACTACTTCGCCATTTGCATTACATCTCTTCTCTATAACATAATAAACTCTTCACACTTTACCCATTCTCTGCAAACTCTCGTTTCTTCCATGGATTCCTCCGCCGCCATTCGATCTTCTCACTGCTTTATGTCCCATATGCAATCCTCATTTCAGAAACCAGTTGTAATTCCCATAAAAATGTTGGATGGAACTTTCAAAGTAGGACTTTCATCCAGAGCTTGGCAGTTAGTGTTAAGCACAATGATTCTCACAACAAATGGAAGAAAACACTAGTGTCTTGCTCAAAACCAACAGAAGCTGTTGACACTACCAAGTCCGAGGCTTCTTTGGACAACATTACAAAAGAATCATTGGAGAAAAATCCTTTGCAAACAGCTACATTTCCTAATGGATTTGAGGCTTTACTATTAGATATATGCGATGAGACTCAAATTGCTGAAGTGAAATTAAAGGTTGGAGAGTTTGAGATGCATATGAAACGAAGCATGGGAGTAGCAGATGCTCCAATTTCCGACATTTCACAAGCTACACCATCGCCTGTTCCAAACTAAACTCATAGTTGAATCAACAACTTCRACCCCACCACCTAAACCATCTCCAGGAAAAACCAATCCATTTGTAAATGAGTTCCATGATAAGTCACCAAAGTTGGCTGCATTGGAGGCATCTAAGAMCMAAAATTATCTCTTGGTAGAATCTCCTATGGTATGSYWTATWWCGAAGAGGTAAAACTGTGAAAGGGAA
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00418 Ps_contig_mira-and-tgicl-ass_franssen_4376
SEQUENCE=GATAGAAAAAAGAAAAGAAAAAAGGAAAAGAGAAAAGCTGTACAGCTTTTCCTCCCCCACCTTCCTTCCTTCTTCGCCACCCACACAAAACGACAACCGCCGCTGCTCCCCTTTCCACTTCCATCATTGCCAACACCTCCACGTAACCGTTCTCCGTCCGCAACCCATCTTCATCATTCATCTCCACCGCAAAAACCCACACGTCGGCGGACTCAAACAACCACACAACACACGTCGCCGGCAAAAATCCTTCTCCAACCGCCGTGACCTAAAGCCGACGAACACCACCATCGCAACGCCCTTCTTCCGCTTCACCCTCACAAACCCTCCTTCTCCGAATCTCCTTCGCCAACAAGGAAAATCCGACCGCCGTACCACCACACACGCGACTCGCATCTCCGATCTACTTTTCCATTTCGATTTCAACTTCACCCTTCCACCATCACAGTTTCACCATTACATACCCTTGAAACCACCTTCAATCTATTCAATCTA
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.2099
PRIMER_LEFT_PENALTY=3.103576
PRIMER_RIGHT_PENALTY=0.106310
PRIMER_LEFT_SEQUENCE=GTACAGCTTTTCCTCCCCCAC
PRIMER_RIGHT_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT=39,21
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=62.104
PRIMER_RIGHT_TM=59.894
PRIMER_LEFT_GC_PERCENT=57.143
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=3.3169
PRIMER_LEFT_1_PENALTY=3.210543
PRIMER_RIGHT_1_PENALTY=0.106310
PRIMER_LEFT_1_SEQUENCE=CTGTACAGCTTTTCCTCCCC
PRIMER_RIGHT_1_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_1=37,20
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=58.789
PRIMER_RIGHT_1_TM=59.894
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=3.3193
PRIMER_LEFT_2_PENALTY=3.213030
PRIMER_RIGHT_2_PENALTY=0.106310
PRIMER_LEFT_2_SEQUENCE=CTGTACAGCTTTTCCTCCCCC
PRIMER_RIGHT_2_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_2=37,21
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=62.213
PRIMER_RIGHT_2_TM=59.894
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=3.4768
PRIMER_LEFT_3_PENALTY=3.370497
PRIMER_RIGHT_3_PENALTY=0.106310
PRIMER_LEFT_3_SEQUENCE=TACAGCTTTTCCTCCCCCAC
PRIMER_RIGHT_3_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_3=40,20
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=61.370
PRIMER_RIGHT_3_TM=59.894
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=3.4768
PRIMER_LEFT_4_PENALTY=3.370497
PRIMER_RIGHT_4_PENALTY=0.106310
PRIMER_LEFT_4_SEQUENCE=GTACAGCTTTTCCTCCCCCA
PRIMER_RIGHT_4_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_4=39,20
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=61.370
PRIMER_RIGHT_4_TM=59.894
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_00419 Ps_contig_mira-and-tgicl-ass_franssen_4402
SEQUENCE=GACTACAACAACAAGAATCACCTTCTGTACCCARWMRRKKYAATTTGACTTCTTTTTCTCTAACCCTTCTTCTTCTTCTTCTTCACCGATTCTTCTGAACCTGAAAATACTACAACATTGCCACATGAATAATTAGGGTTTACACACAACAAAGTTGAAACGATTTCCGAAATGAGCGGTTCCAGATTCATAAAGTGTGTCACAGTAGGCGATGGAGCCGTTGGCAAGACTTTGTTTGCTTATCT
TARGET=66,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00420 Ps_contig_mira-and-tgicl-ass_franssen_4414
SEQUENCE=GAATATTCAATTTCTATCCACTATTCATCTTCTTCTTCTTCTGTTTCTCATCTAGGGTTTTCTATTCCATTTCCATGGCCACCGCAATTCGAACTCCCTTCTTCTCCTCCCACCACCACCA
TARGET=28,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00421 Ps_contig_mira-and-tgicl-ass_franssen_4414
SEQUENCE=GAATATTCAATTTCTATCCACTATTCATCTTCTTCTTCTTCTGTTTCTCATCTAGGGTTTTCTATTCCATTTCCATGGCCACCGCAATTCGAACTCCCTTCTTCTCCTCCCACCACCACCA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_00422 Ps_contig_mira-and-tgicl-ass_franssen_4425
SEQUENCE=GGCCACCGGAGCTAACAATTCTCCTGTCAAAACAAATTGACGAGAAAGAGAAGCCGTTAGAAACGGGAGTTTTCCGTAGACGGAAGTTATGCGGCGGAGGAGCGGTGGAGGAAGCGACTCGGAAGATAGTAGCCATGGTAATGGTTTGTTACTCTAGAAGAAGAAGAAGAAGAAGAATTGAGAAATTGAGGAAGAGGAGAATAATTAGGGTTAAGGAATTGCGTGAGGGAGGAGAAAGAGAAAGAGAAAGGGAAATGGTATTTCCTTTCTTTCTTTCTTGTTGGTTTTGATTCACTCCCTCACTCACAAGAGAACTCAGACACTCTCCCCGAGTACTCTGCGTTG
TARGET=156,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0283
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.025669
PRIMER_LEFT_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT=56,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1482
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.025669
PRIMER_LEFT_1_SEQUENCE=GGAAGCGACTCGGAAGATAGTA
PRIMER_RIGHT_1_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=4.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.1542
PRIMER_LEFT_2_PENALTY=0.002623
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_2_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=59.997
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.1680
PRIMER_LEFT_3_PENALTY=0.142283
PRIMER_RIGHT_3_PENALTY=0.025669
PRIMER_LEFT_3_SEQUENCE=CGAGAAAGAGAAGCCGTTAGAA
PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=60.142
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.2577
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.025669
PRIMER_LEFT_4_SEQUENCE=CGACTCGGAAGATAGTAGCCAT
PRIMER_RIGHT_4_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_4=114,22
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.026
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00423 Ps_contig_mira-and-tgicl-ass_franssen_4425
SEQUENCE=GGCCACCGGAGCTAACAATTCTCCTGTCAAAACAAATTGACGAGAAAGAGAAGCCGTTAGAAACGGGAGTTTTCCGTAGACGGAAGTTATGCGGCGGAGGAGCGGTGGAGGAAGCGACTCGGAAGATAGTAGCCATGGTAATGGTTTGTTACTCTAGAAGAAGAAGAAGAAGAAGAATTGAGAAATTGAGGAAGAGGAGAATAATTAGGGTTAAGGAATTGCGTGAGGGAGGAGAAAGAGAAAGAGAAAGGGAAATGGTATTTCCTTTCTTTCTTTCTTGTTGGTTTTGATTCACTCCCTCACTCACAAGAGAACTCAGACACTCTCCCCGAGTACTCTGCGTTG
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0283
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.025669
PRIMER_LEFT_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT=56,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1482
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.025669
PRIMER_LEFT_1_SEQUENCE=GGAAGCGACTCGGAAGATAGTA
PRIMER_RIGHT_1_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=4.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.1542
PRIMER_LEFT_2_PENALTY=0.002623
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_2_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=59.997
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.1680
PRIMER_LEFT_3_PENALTY=0.142283
PRIMER_RIGHT_3_PENALTY=0.025669
PRIMER_LEFT_3_SEQUENCE=CGAGAAAGAGAAGCCGTTAGAA
PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=60.142
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.2577
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.025669
PRIMER_LEFT_4_SEQUENCE=CGACTCGGAAGATAGTAGCCAT
PRIMER_RIGHT_4_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_4=114,22
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.026
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00424 Ps_contig_mira-and-tgicl-ass_franssen_4459
SEQUENCE=GAAACTGTGAGACTTTCCAGCTGCCATTTCAACGCCAAAACAATCATCTACAGAGCTATTTTCAATGTCAAACGCAGTCCATCTGGGGTGTTGTTGAAGGAGGATAGTGTGATGAGAAGGTTGTTGAAGAAGGAAAGGAGAAGAAGAAGAAGAATGATGATGAGATTGGGAGTCGAGGAGGTGCTGAAGCTGACCGATTAGCTGCTTCAGCATGGCCAGGCCTTCCATCGCCCCCCCCGCGTACTTCCCCCGCAAT
TARGET=139,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1659
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=1.029845
PRIMER_LEFT_SEQUENCE=GGGTGTTGTTGAAGGAGGATAG
PRIMER_RIGHT_SEQUENCE=CTAATCGGTCAGCTTCAGCAC
PRIMER_LEFT=85,22
PRIMER_RIGHT=200,21
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=1.2971
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=1.161090
PRIMER_LEFT_1_SEQUENCE=GGGTGTTGTTGAAGGAGGATAG
PRIMER_RIGHT_1_SEQUENCE=CAGCTAATCGGTCAGCTTCAG
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=203,21
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.161
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=1.4713
PRIMER_LEFT_2_PENALTY=0.481423
PRIMER_RIGHT_2_PENALTY=0.989871
PRIMER_LEFT_2_SEQUENCE=CAACGCCAAAACAATCATCTAC
PRIMER_RIGHT_2_SEQUENCE=CTCGACTCCCAATCTCATCATC
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=176,22
PRIMER_LEFT_2_TM=59.519
PRIMER_RIGHT_2_TM=60.990
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=1.5076
PRIMER_LEFT_3_PENALTY=0.481423
PRIMER_RIGHT_3_PENALTY=1.026160
PRIMER_LEFT_3_SEQUENCE=CAACGCCAAAACAATCATCTAC
PRIMER_RIGHT_3_SEQUENCE=TCGACTCCCAATCTCATCATC
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=175,21
PRIMER_LEFT_3_TM=59.519
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=1.5113
PRIMER_LEFT_4_PENALTY=0.481423
PRIMER_RIGHT_4_PENALTY=1.029845
PRIMER_LEFT_4_SEQUENCE=CAACGCCAAAACAATCATCTAC
PRIMER_RIGHT_4_SEQUENCE=CTAATCGGTCAGCTTCAGCAC
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=200,21
PRIMER_LEFT_4_TM=59.519
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_00425 Ps_contig_mira-and-tgicl-ass_franssen_4464
SEQUENCE=TTCATGCAACCACTGCTTGACATTGTTGAAACTTTCCATATCAGTTACATCATAAACAATAATGATTCCATGTGCTCCTCTATAATAACTGCTAGTTATAGTCCTGAATCGCTCTTGTCCAGCAGAAGTCACAATTTGCAGTTTGACGGTTTTGCCTTCAAGTTCAACTGTTCTGATTTTCTGGCATAAAAAGTGTGAATGAGTATATAATTGATGGATAGAATAGAATAGAAAAGAATAGAAAATATAGTTACAAAATCGACGCCAATGGTGCTAATGTAGCTGTCAACATAAGAGTCATCCTAGAAAAAAAGTTAGGGTTAGGGATGGAAAAGGTCAAGTAAGTAAAAAGGATGTGAGTAGAAAAGGAACGGACGGCGAATCTGAGAAGCAGGA
TARGET=218,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2892
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.069319
PRIMER_LEFT_SEQUENCE=TGTCCAGCAGAAGTCACAATTT
PRIMER_RIGHT_SEQUENCE=ACCTTTTCCATCCCTAACCCTA
PRIMER_LEFT=115,22
PRIMER_RIGHT=336,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=60.069
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3530
PRIMER_LEFT_1_PENALTY=0.283676
PRIMER_RIGHT_1_PENALTY=0.069319
PRIMER_LEFT_1_SEQUENCE=CAACTGTTCTGATTTTCTGGCA
PRIMER_RIGHT_1_SEQUENCE=ACCTTTTCCATCCCTAACCCTA
PRIMER_LEFT_1=164,22
PRIMER_RIGHT_1=336,22
PRIMER_LEFT_1_TM=60.284
PRIMER_RIGHT_1_TM=60.069
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.3844
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=TGTCCAGCAGAAGTCACAATTT
PRIMER_RIGHT_2_SEQUENCE=CGTCCGTTCCTTTTCTACTCAC
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=376,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=0.4482
PRIMER_LEFT_3_PENALTY=0.283676
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=CAACTGTTCTGATTTTCTGGCA
PRIMER_RIGHT_3_SEQUENCE=CGTCCGTTCCTTTTCTACTCAC
PRIMER_LEFT_3=164,22
PRIMER_RIGHT_3=376,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.6680
PRIMER_LEFT_4_PENALTY=0.598673
PRIMER_RIGHT_4_PENALTY=0.069319
PRIMER_LEFT_4_SEQUENCE=TTGCCTTCAAGTTCAACTGTTC
PRIMER_RIGHT_4_SEQUENCE=ACCTTTTCCATCCCTAACCCTA
PRIMER_LEFT_4=151,22
PRIMER_RIGHT_4=336,22
PRIMER_LEFT_4_TM=59.401
PRIMER_RIGHT_4_TM=60.069
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_00426 Ps_contig_mira-and-tgicl-ass_franssen_4469
SEQUENCE=AGATGATATAACCTTCAAATTTGCTTATCATGTTTCAATGATGATCACAAAGTAAAATCAAAGACAACACAAAATATCRAATATTCCACATCATTAATAACCTTTTAGAATCTACATTCTTCTCGCCCGGGTTAATAAACACAATTCTATATATTGGATTTGTTGCAATGATAACTGAATAAAAAGGAAAAGAAAAGAAAAATCAGTAGTTG
TARGET=187,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00427 Ps_contig_mira-and-tgicl-ass_franssen_4492
SEQUENCE=AAGTAAATAACAAAACGACCCAAACAAAAAATAGTAATTTAAATTTTAAACTAAAAGTTTAACAGCAGACAACTAGCACTTAATAATTTCGTCCTATTACAATGAGATTATTCTTATTTTGTATACCAAATGTCAAGATTATTGCAATGTAGTACTCATACACATACATAATAAGAACATAATGTATTATAAATGTTTTTGGTCCATTATCCCAAAGCCAATGTGTAACTCATTCCTTCCATCTCTGCTCATGAGCAAATGAAGCTTCAAGAGCACATTGCCTTCTCCACAACTTCCTGTAATTCTCCATTCTTATATGCCTCAACAGTGATATCACATCCACCGAAAAACTCTCCATCAATGTATAGCTGAGGAAAAGTAGGCCAACTGCTATACTCCTTAAGTCCCTGGCGCACCAAATCATTATCCAATATGTTTACAGTTTCAAAAGAAGCATTCAAAGACTTCAAAATTTGCACCACTGTATTTGAGAATCCACACTGTGGAAAATCCCTAGTACCCTTCATAAACAAAACTATTTTGTTTGAAGTAACAACTTTATCCAACGTGGTCTTCAATTCAGGAGTCAACGCAGAGCAGCGAGTTGTGGTGGAAGAGCGTTTGAGATGTAGTTTGGGTTGGGAATGGAAAACGGTGTTTTGAGAGAGAAGAGGAGATGAACGAGGTAGTAGAGACAGGGACGAGGTTGTTGTTGTTGTTGTTGTTCCAGAAACGTGGGAGTGGAAGCATGATGTTGTCGGGTTGAATTGTAGCTGTGAAGAAATTGGGTTAACGCACCATGTGAAAGACATGTTCTGAGTTCTAACTTCCACTCACAATTCTCTTCCAACAAATATCACTTGTTTGATTTCTCCGATTTTTTTTTCTCAACTCCCCCGAC
TARGET=706,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3081
PRIMER_LEFT_PENALTY=0.038090
PRIMER_RIGHT_PENALTY=0.269968
PRIMER_LEFT_SEQUENCE=ACGTGGTCTTCAATTCAGGAGT
PRIMER_RIGHT_SEQUENCE=TACAATTCAACCCGACAACATC
PRIMER_LEFT=565,22
PRIMER_RIGHT=771,22
PRIMER_LEFT_TM=60.038
PRIMER_RIGHT_TM=59.730
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.3269
PRIMER_LEFT_1_PENALTY=0.038090
PRIMER_RIGHT_1_PENALTY=0.288804
PRIMER_LEFT_1_SEQUENCE=ACGTGGTCTTCAATTCAGGAGT
PRIMER_RIGHT_1_SEQUENCE=TTGGAAGAGAATTGTGAGTGGA
PRIMER_LEFT_1=565,22
PRIMER_RIGHT_1=849,22
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=59.711
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.4139
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.269968
PRIMER_LEFT_2_SEQUENCE=GGAAAACGGTGTTTTGAGAGAG
PRIMER_RIGHT_2_SEQUENCE=TACAATTCAACCCGACAACATC
PRIMER_LEFT_2=646,22
PRIMER_RIGHT_2=771,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=59.730
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.4328
PRIMER_LEFT_3_PENALTY=0.143968
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_LEFT_3_SEQUENCE=GGAAAACGGTGTTTTGAGAGAG
PRIMER_RIGHT_3_SEQUENCE=TGGAAGAGAATTGTGAGTGGAA
PRIMER_LEFT_3=646,22
PRIMER_RIGHT_3=848,22
PRIMER_LEFT_3_TM=60.144
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.4328
PRIMER_LEFT_4_PENALTY=0.143968
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_LEFT_4_SEQUENCE=GGAAAACGGTGTTTTGAGAGAG
PRIMER_RIGHT_4_SEQUENCE=TTGGAAGAGAATTGTGAGTGGA
PRIMER_LEFT_4=646,22
PRIMER_RIGHT_4=849,22
PRIMER_LEFT_4_TM=60.144
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_00428 Ps_contig_mira-and-tgicl-ass_franssen_4493
SEQUENCE=GACAATCATTAATATACATGGCTAGAATGAAAATAACAAGATTCGCCACAACAAACAAAGGAACAAGCCATGATGTCCAACTAGTTTTCTTGGATCATAAACACGTGAAGGAGGAGGAGGAATAGAAGAAGAATTAGTTCTTTGTTTCGCCCCTTCTCTCTCAAGATCCTCCTAACCAGTACTTATTTTTCTCTCTCTACGAACCAGAGAGAACTAAATTTACGTAAATTTAGTAGGAACGTAAAGGGTTTGTAGGTAGTGGAAGAAGGAGTAGGGTAAGGGGTTGGGGTTT
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5344
PRIMER_LEFT_PENALTY=0.306698
PRIMER_RIGHT_PENALTY=0.227726
PRIMER_LEFT_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_RIGHT_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT=46,22
PRIMER_RIGHT=285,22
PRIMER_LEFT_TM=59.693
PRIMER_RIGHT_TM=60.228
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.6521
PRIMER_LEFT_1_PENALTY=0.424392
PRIMER_RIGHT_1_PENALTY=0.227726
PRIMER_LEFT_1_SEQUENCE=ACAACAAACAAAGGAACAAGCC
PRIMER_RIGHT_1_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=60.424
PRIMER_RIGHT_1_TM=60.228
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.6585
PRIMER_LEFT_2_PENALTY=0.306698
PRIMER_RIGHT_2_PENALTY=0.351777
PRIMER_LEFT_2_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_RIGHT_2_SEQUENCE=AGGGGCGAAACAAAGAACTAAT
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=153,22
PRIMER_LEFT_2_TM=59.693
PRIMER_RIGHT_2_TM=60.352
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.6757
PRIMER_LEFT_3_PENALTY=0.447971
PRIMER_RIGHT_3_PENALTY=0.227726
PRIMER_LEFT_3_SEQUENCE=CCACAACAAACAAAGGAACAAG
PRIMER_RIGHT_3_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=285,22
PRIMER_LEFT_3_TM=59.552
PRIMER_RIGHT_3_TM=60.228
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.7290
PRIMER_LEFT_4_PENALTY=0.501224
PRIMER_RIGHT_4_PENALTY=0.227726
PRIMER_LEFT_4_SEQUENCE=AACAAGCCATGATGTCCAACTA
PRIMER_RIGHT_4_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=60.228
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_00429 Ps_contig_mira-and-tgicl-ass_franssen_4534
SEQUENCE=CTAATRGTAAGAAAATTGTGTCCAAGATTAAGTTCGAATAGAAAATTTCTTGAGTACAGATGTGATGCATAATCTAAATTAAAAGTTACATTCAAGCTTAAGAAAAGACTTCATAAATTTATTACTTTCTCTATGGTACAAGAAAGAAAGTTTATTGGATCAAACGAAACTACACATGAAGAAAAGTAGACAAATGGAAATAAATATATATATGATCAANAATTGGATACACATATAGAACATTAAA
TARGET=204,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00430 Ps_contig_mira-and-tgicl-ass_franssen_4542
SEQUENCE=AGAAAAACCATAAGAGCTACATCTGCATCACATAGAGTTGAAAGCTCCTGAGCTTTCTTGAAAAGCCCTTTCCTCCTCTTAGAGAAAGTCACTTGCCTTGAACTGATGTTGTCAATTTTCTTGATCTGTATCTTCTTTCTTGTCATACTATTATTATTCCTCTCTCTCTCAAAATTTAGGTTACTTTATGAACTATGAAGAGAGAGAGAAAGCGGAAGTTACAAGAACAAACCGAACCAGTTTMTCTTATTGAGATTCCCCGA
TARGET=160,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00431 Ps_contig_mira-and-tgicl-ass_franssen_4542
SEQUENCE=AGAAAAACCATAAGAGCTACATCTGCATCACATAGAGTTGAAAGCTCCTGAGCTTTCTTGAAAAGCCCTTTCCTCCTCTTAGAGAAAGTCACTTGCCTTGAACTGATGTTGTCAATTTTCTTGATCTGTATCTTCTTTCTTGTCATACTATTATTATTCCTCTCTCTCTCAAAATTTAGGTTACTTTATGAACTATGAAGAGAGAGAGAAAGCGGAAGTTACAAGAACAAACCGAACCAGTTTMTCTTATTGAGATTCCCCGA
TARGET=199,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00432 Ps_contig_mira-and-tgicl-ass_franssen_4560
SEQUENCE=AATAYTAKTAGTTCTAAGAAACCTTACCATTCAACCTCAAAATTTACATTCCCGAAATATTAATGTACAGTTGTACTTTGTTTGTGAGTACATAAAACTACACATGTTCCATATAAAAAAACACAAGGATTTAACTAAAATTCTAAACCAATCACAGAACATGCAAACCAAGTTTTGTTTCTTAAACGTTCTGATCCGTTGATGTTGTTGTTTCTGTTGTAGTTGTTGCTGCTGTTGTTGTTCTTTCGACACTCTCTGTTCTTGGAACACGATTTTCATCACCACTTCCTTGTTTCACACTTGAATCACCATCATCATCATCGTTATCGTTATTGTTATCCGCATCATCGTTCATGTTTTCCTTTAATTTCTGGTTCTGGTCACAGGTACATGTACTAGTGTTATCGCCGAAAGACGAGGATCTACTAGTACCTACGCTTAATGAAGACATGCTTGGTGTTGCTAAGAATGTTGGTTTGGCTTGTCCCGCCATAATCACCAAGATTTTCTCCTCGTAAGGCTTCTGAGGTTTTTGATCTGTGTCGTTTGTTTCTTGCCCAGCTTCAAGGTCTCGTTCGGATTCGCCGTTGCCTTCGAGGTAACCAGAGAGTTTCCAATAAGAACATGCAAGGATTAAGAGAGCGAAAGCTATGAGGCCTAACATGGCTGASCTAAAACCCACCGAAGGAGGGTAAGGGACTGGTAGAATGCCAGTGGTAGG
TARGET=310,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00433 Ps_contig_mira-and-tgicl-ass_franssen_4563
SEQUENCE=GCCAAGCTTTAGTAATACGCTGAGGACCGCGTACACTGACTCTTGTTATTGTGGCGTTTCTTCTTGTTGCATTGTCGTCTGTTGATTCGGCTCTCAGCTACCTTTTGAATTTAGCTCTTCGGAAAAGCACATAACAGTAGCAGTATTGCTCATGTTACCATTACTATCTATTCAAGTAGCTCTTATCGAAGCCGTGGATTCAGGTGAGGAAATTGGTTGCATTGCATGGAATCTTGGTAACATAATTTCATGCATAGAAACTGTATATTGAAATACATATTTGAGGATTATAAGTTTGAAGTGTAAAAAATCGGTTGTATTAAACTGAAAAATCAATTAAGTTCCGCATCGATTAGTGTGTGTGTGTGTGAAAAAATAGAACTTGAGAGTTATTATTCATATGAATAGTACTCCCTCCGTTTCTTTTTAAGTGTCGTTTTAGCATAAAGCTCTCCAACCAAAG
TARGET=356,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3183
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.256503
PRIMER_LEFT_SEQUENCE=AGTAGCTCTTATCGAAGCCGTG
PRIMER_RIGHT_SEQUENCE=CTTTGGTTGGAGAGCTTTATGC
PRIMER_LEFT=174,22
PRIMER_RIGHT=462,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=60.257
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.4209
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.359050
PRIMER_LEFT_1_SEQUENCE=AGTAGCTCTTATCGAAGCCGTG
PRIMER_RIGHT_1_SEQUENCE=CACTTAAAAAGAAACGGAGGGA
PRIMER_LEFT_1=174,22
PRIMER_RIGHT_1=432,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=59.641
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.4775
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=0.256503
PRIMER_LEFT_2_SEQUENCE=ATTCAGGTGAGGAAATTGGTTG
PRIMER_RIGHT_2_SEQUENCE=CTTTGGTTGGAGAGCTTTATGC
PRIMER_LEFT_2=197,22
PRIMER_RIGHT_2=462,22
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=60.257
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.5800
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.359050
PRIMER_LEFT_3_SEQUENCE=ATTCAGGTGAGGAAATTGGTTG
PRIMER_RIGHT_3_SEQUENCE=CACTTAAAAAGAAACGGAGGGA
PRIMER_LEFT_3=197,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.641
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.7758
PRIMER_LEFT_4_PENALTY=0.519311
PRIMER_RIGHT_4_PENALTY=0.256503
PRIMER_LEFT_4_SEQUENCE=CAAGTAGCTCTTATCGAAGCCG
PRIMER_RIGHT_4_SEQUENCE=CTTTGGTTGGAGAGCTTTATGC
PRIMER_LEFT_4=172,22
PRIMER_RIGHT_4=462,22
PRIMER_LEFT_4_TM=60.519
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_00434 Ps_contig_mira-and-tgicl-ass_franssen_4574
SEQUENCE=GGAACTAATAACTCTAAATTTTATTAAGTCATAAAGTAGAAGTATCTAACTCTTCCAAACATGGATTAAATTGGATCAAATAAGAATACCAAACACACTACCTTAAATTAAACTAACAAAACAAATCATGTGAATATGTGAATCACCTAACAACAAACAACTAACAATAAAAAATACGAAGGGGGTAGGAGTTCTACACCTATTTTTTCCAACAGGCAATAGGTGAGCAACAAACAAACAGACAAATAGAAGTAATAGTAACTTAAG
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00435 Ps_contig_mira-and-tgicl-ass_franssen_4585
SEQUENCE=GATTGYGATTGCAGATAAGAAAGAGTTTCTTCTCTCTTCACCGCCTCAAACCACTCTTCCTATTTTCTCTCTCTCTATTGTCTCTGATTTACAACTAATAACAGTGTAGTTTAGTATCATCATCATTCATCCGGACAGATAAAAACAGAGTCCTCTGTTTCACAGAGTGTTGTGTTGTGTTCTGTTTCCTGCTCTCTCTCAGGTCACTCACAGGCTCTCACCTTTGTTTGGATTCACTGATATTTGAAAAATAGCAGCAGCAGCACCTGATGCAGATGCAGCCTATGATGGCAGCTTACTATCCCAACAACGTCACCACTGATCATATTCAACAGTACCTGGACGAGAACAAGTCCCTGATTCTCAAGATCGTCGAAAGCCAGAACTCTGGCAAACTCACCGAATGTGCCGAGAACCAATCAAGGCTTCAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCCCAACCACCTACCATGTCTGGTCAGTATCCTTCAAGTGGAATGATGCAGCAGGGAGGACACTACATGCAGGCTCAGCAAGCTCAACAGATGACACAGCAGCAACTGATGGCTGCGCGTTCTTCTCTACTGTATGCTCAACAACTTCAACAGCAGCTTGGCATGAATTCCAGTGGAACTCAAGGTCTTCACAT
TARGET=66,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00436 Ps_contig_mira-and-tgicl-ass_franssen_4585
SEQUENCE=GATTGYGATTGCAGATAAGAAAGAGTTTCTTCTCTCTTCACCGCCTCAAACCACTCTTCCTATTTTCTCTCTCTCTATTGTCTCTGATTTACAACTAATAACAGTGTAGTTTAGTATCATCATCATTCATCCGGACAGATAAAAACAGAGTCCTCTGTTTCACAGAGTGTTGTGTTGTGTTCTGTTTCCTGCTCTCTCTCAGGTCACTCACAGGCTCTCACCTTTGTTTGGATTCACTGATATTTGAAAAATAGCAGCAGCAGCACCTGATGCAGATGCAGCCTATGATGGCAGCTTACTATCCCAACAACGTCACCACTGATCATATTCAACAGTACCTGGACGAGAACAAGTCCCTGATTCTCAAGATCGTCGAAAGCCAGAACTCTGGCAAACTCACCGAATGTGCCGAGAACCAATCAAGGCTTCAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCCCAACCACCTACCATGTCTGGTCAGTATCCTTCAAGTGGAATGATGCAGCAGGGAGGACACTACATGCAGGCTCAGCAAGCTCAACAGATGACACAGCAGCAACTGATGGCTGCGCGTTCTTCTCTACTGTATGCTCAACAACTTCAACAGCAGCTTGGCATGAATTCCAGTGGAACTCAAGGTCTTCACAT
TARGET=167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00437 Ps_contig_mira-and-tgicl-ass_franssen_4585
SEQUENCE=GATTGYGATTGCAGATAAGAAAGAGTTTCTTCTCTCTTCACCGCCTCAAACCACTCTTCCTATTTTCTCTCTCTCTATTGTCTCTGATTTACAACTAATAACAGTGTAGTTTAGTATCATCATCATTCATCCGGACAGATAAAAACAGAGTCCTCTGTTTCACAGAGTGTTGTGTTGTGTTCTGTTTCCTGCTCTCTCTCAGGTCACTCACAGGCTCTCACCTTTGTTTGGATTCACTGATATTTGAAAAATAGCAGCAGCAGCACCTGATGCAGATGCAGCCTATGATGGCAGCTTACTATCCCAACAACGTCACCACTGATCATATTCAACAGTACCTGGACGAGAACAAGTCCCTGATTCTCAAGATCGTCGAAAGCCAGAACTCTGGCAAACTCACCGAATGTGCCGAGAACCAATCAAGGCTTCAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCCCAACCACCTACCATGTCTGGTCAGTATCCTTCAAGTGGAATGATGCAGCAGGGAGGACACTACATGCAGGCTCAGCAAGCTCAACAGATGACACAGCAGCAACTGATGGCTGCGCGTTCTTCTCTACTGTATGCTCAACAACTTCAACAGCAGCTTGGCATGAATTCCAGTGGAACTCAAGGTCTTCACAT
TARGET=253,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00438 Ps_contig_mira-and-tgicl-ass_franssen_4604
SEQUENCE=TTACAACATACAACATCTTAAAAGGTTAATACTAGATATATTAAACAAAAGGTTCATAATCTCCTGCTCATAACTCAGTTAACTAGTGAAGATATGTAGCTTTGGTTACTGATAGCCATTTCCAAAGTAGTTTCCATGATTATGAGAGAAGTTATAATGTGCGTTGCCGAGGATAGGGGGACAATGATTGTCGGTTGGTCCTGCGTACATGTAAGGTCGGTCGTACACATATTGTGGGTACCTGTCAGCAGCAACTCTAGCATAGGAAGTTTTGTCAGAAGGGATGTTGTTAGTGACACGGGGACCATATCCTACACCGTTAGCACGAGGTCTCTTTGGTTGAGGCTTTGTCGCTTCAGTTTCCCTTTTTTTGTCAGCTTTCGCCTTCTCTAGTTGAACAACTCGTTTCACGAGAGGTTCCAGAGGATACTGCTCTTCGAGTTTATGTTCTTCAATGCACTTGATTACAGCTTTATGAGCAATCAGCTCTCGTTCATTAACCTCAATCTGTGCAGTGGGGGATGAGTTACCCCTTTTGACAGGTGAGCAAGAATTTCTAGCATCCTTCAAATAAGATTTCAGAAGTGAAACAGGAGAAAACTGTTTTGTCAGATCAAATGCAAAAGCCAAGTTAACCGCGTCAATTTGCCTCCCACTGTTCACCAAAACCTCAATTACACCAGGCATCTTCTCTGATAATCCAAGGCAACGGCATAAATCAGCAGTTTGGCGACGCCGAGACACCATTGGAATTAGCCTGGATAACTCTTCCTCGTCAAAACCAGACGCAATATCAAAACTAGCTACGAGTTGCAAAAACGCATGAGCCTCCAAGGAATTCCCATTGCTAGCATCGATGTCAAGCGCATCCAATCTTGGCTTCCACTCTTCAGCAACTGCCTTTGCCCTATCCTTGATATCTTTGGAAATCACATTAGAAACGGAATGCAAATCGGTTAGGAAATCACTCAGACACTCCATCAGCATAATACAGGTCCGGCGAAGACCTAATAAGTTCGCATCTTTCTTAATATCCTGACTAGACACTTCCGTGCAGTAAAATCCTTCCAAAGAATCTAACACCAGACAGGCAGCATTAGGAGAAGCTCTTAACGCAAGTGGTATTTCCTCCCTTATAGCAGCAAGGTTCTTACGGTTATCAGATATGAATTTGTGAAGTCCAGAAACATCCATCTCTTTACAAAGTTTTACCAATTCTGGATAAGAGATCAATTCCACATTTCTATCTTCACGAGAAACTTTCACATCTTCTACGCGACCTTCCGTCACATTAGAAATAGCATCTGCATCCATCGGTTTCTCCTCCACACGTAATGCACATTGACCCCCATTGGAGACGATGCCCAAATCTTTTTGCAAAACCTTCCTGTGCTTTTCTCGAGCATTTACAATCGCAAATGTAGCAGCATCTCTTTTCTCTTGAAGCCTCTGCAATGTGGCTTGCTCCTGGGCAAAAATAGCCGCTTCCTGCTTCTTCAACATTTCACGAGCTTTCAGTGTTTTGTTCTCAAACACTTTTTCTTGATCTTCTAATTCGTGAAAGCGCCTCTTCAAGGATTTCTCAAGCCCGTGAAAATGTTCCTCCAGTTCTTTCCATTTCATGTTAAGAGTAACCGCTCGATAACTTTCGAGTTCAGCAAACGCCTTCTGCAGCTGTTGTATCTTCGAAGACGTAGAATCCATCAGTGTAGCAACCGAGTTTGTTTCTTCCATTGCTAAGACGCTGATTGATCTAGAAAAGAAACTAGTACTAGTAAAAACAAAGAGGAGGGAGAGGGAGAGATAGAGAGAAAGAGAGAGAGAGTTCTCAATCCATATT
TARGET=1814,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00439 Ps_contig_mira-and-tgicl-ass_franssen_4609
SEQUENCE=ACGCAACAAAAATTCATTGATTATTAAACATCCACTACATCTTACAAGACCTACAACAAACATCAAGAAATTAACATACTATCCTGCATATCCTTCTCATCCATAAGAACAAAAAAATATATACAGAAGCCTAAAAAAAATAGAAAACCATATTACATACTATAGTATATTTATCATTAATTCATAGGTTAATGAAATTCAAGAGCCCTATACTATACTCAAGATTATTATTATTAATTAAAACACTTGAAATCAAATCATTCAACAGGAAGGAACGACGCCGTATCCATCATAAGACTCATCCTCACCGATTAGATTCACATTCATATTCAAATCAGTGCGTTTTGCGAACCTTCCTTTAATTCTAGGTCTAGTTTCTGCATACGCTTTTCTCGAAGCGTAACGAATTGTCTTCTCGAACCTTCTATTCTTTCTCTTCTCTCTGTACCTCATAACCCTAGCTTCTCTATCCGCCGCCGTGATTTGCACCGCCTCCGTCCTTCCGTAACCGCAGTATGACATCTCCGACATCACGTTTCCGTCTGGTACAACTCCAACCTCCAGCGAAGGTGACGATACGCTGTGATTGTAGTTGTAAGTAAAAGGTTTAGAAGTAGAGAAATCGTTGTTGATATCGGAGTAGTGTTCGGTGACAGTTTTGCTATGAGACTGCACCGGCACAACTCCGTCTGCGGTGCCGGGACTATGTTGCTCCGGCGAGTCAGTTTTGGCGTCAACACAGACGAAATTGAGGTCGGTAGCGTCGATTTCGGTGAATGAGTAGTGAGATGAGTTGAGATCGGGGCCTTTAGGGTTGGCGGGAAGTTTGGAGGAGCCAGGAAGCAGCTTCGGCTTCTTCGGTGCTGACGTCAGCATCTGGATCGGATTCGGAGAAGAATTGTTGCTTGCTGTTGTGGTGGAAAGTGTTGAGCGGGATGCGTTCGTGGCGGCTAGCGRGGGGATTGGCGGAGTGGATGTCGTGGTCGCAGGAGACGCAGAGAGCAGCGGCGTCTGCTTTGCATGTGACGTGAGCAGGAGCTTGTTCACAGACTTCGCAGAGTGTGACGCGGTGATGGCGGGAGGCGAGTTTGTTGGCGGCSTGGATGTTGGAGTCACAGGTGATACAGAGGAAAGCTGAGTCAGAACGGCAGAATAGTGTTGCTTTTGTTGACTTGCAGGAATCACAGAGCTTAGTGGCCATTTGTTGTTTATTGAGTTAGTTAGAAGTTTGAGAAGTTGTGAGTGTATTGGCGAAGTGTTTTGGTTGGATTG
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00440 Ps_contig_mira-and-tgicl-ass_franssen_4628
SEQUENCE=TACGGCGGGTTTGGTAAATCAAGTAGTCTCCGGCCTTCGGTTCCTAGACCAAATTACGACGAATTCAAACGAAAGGAAGGAAGGAGAAAACAAAATAGGTGGAAATTACTTCAACTGGTTCTTCTTTTTCTATAAGCATAGGTTCATTAACAGCGGTGACAGTTCTGGTTTACATTCAAGATCATGTGGGAAGAGATTGGGGATAYGGTTTGTGTGCGTGTGCTATTGTTGTGGCGCTTCTTGTGTTCTTGTCGGGGACAAAGAGGTATCGGTTTAAGAAACTTGGTGGGGAGTCCTTTGACTCAGATTGGTCGGGTTGGGTTTTTTGGTGGGCGGCGTGGAGGAAGGAGGGAGGGTTGGAGTTGCCGTATGATTCGTCGTTGTTGTATAATTTGGATGATGTTGAAGNTCATGATACTGAGACCACGCAAACGG
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00441 Ps_contig_mira-and-tgicl-ass_franssen_4633
SEQUENCE=TTTCTACAAAGTCCTACATTTTGAGTTAAAATATTTTAGCACACAAGCAAGCTCAATTATTATTCTGTCTTTTGTAGCCATGTGCTAATTTTTTGTTATGATTCCTCGTGTATTGCTTTTCAAAGCAACTTCATCCATATGGCCTGAATTAATTTATAATAATCTCCTACAATTCATTAATATGGCCTGTGTTAATTTCTAATAATCTCCAACAATTGTATTTTCTGCTTTGCCATGAAAAATCCAATTCATATCTTGTTGCAGAGAAAAGAAGAATATGACATGGGTGGAACTAGAGGGCAAAAGCTTTCTGGAATGCAAAAGCAAGTACTTAGTTTATACAGAGGRTTTCTACGTGCAGCACGTTCCAAATCTGAAGAGGAACGCTGCAAGATAGAATCTGTAATATGTCAAGAGTTTCGATGCAATTCCAAGGAAGTGGATAGGAAGAATTTTCAATACATTGAATATTTACTTCGTCGAGGATATAAACAGCTTGATCAACTTCGGAACCCTGGTACCACTGGCTTATCTTTCTTTGACTAAAACTTAGTAATTTTTACAAAAGTTAAAACTTTAAAATTCTTTTCAATAAAGNTTCTTGTCTAATTTGTTATCCAATTAATTAATTCAGGTA
TARGET=620,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00442 Ps_contig_mira-and-tgicl-ass_franssen_4664
SEQUENCE=ATCTACTACCCACTTTCTAACTTTTAATTACAATAACAAACAACCAAAAAATAAAAATACAACCAAACCAACCAACCCTACCTTGGCCGAAACCCAAAACTACCACAAACCTAATCTCTCTGGCCATCCCACTTCCACCTTATATCTGTAGACTTGAGTACAGTGGAGCAAACCAAAAGCAAACCAAATCCATTTTCTATTGAGTTTCTCTGTTTCCTCAAACTCTACCAAATATACAATAATAATAACAACAACAAAAAAACATTTTTTTATATAAACCTCAAACTCTGCACAAACCATGAAAAATCTCTCTACTGTTCTGAACTTTGAGAATCATCCTTAGTAATATTCTGATTCTGGTTCTGATTTTGCTGATGCACCCTAGTTTGATGATTTTGACCAAACTGCTGATAAATCTGACTCAAAGCCTGGTTATTCAACACACCAATATGTCCATCTTGTGACAAACCTAATTCCAAACCAGGTGGCATCTGTTGATTCCCACCCAAAATTGATGTAAAACTCATCCCAACCCAAATTACGCCCCTGGACATATCAAATCCAGAAAACCCTAATTTTCTAGAAAATAACTTGAA
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00443 Ps_contig_mira-and-tgicl-ass_franssen_4677
SEQUENCE=ATTGTTAGTAAATTTTGGATGTAACAAATACCCTCCCAAGTTTACTCCCCGAAAGTGACGCGTGTACTTCAGAAAGTGAAGCGTCACTAGTTGAAAATTCCATGTCCGGGATTCGACTGTATGCTCCGGCTTTATCAATTAGAATAGTTCCAATTTTTCACCATTACCTCATTCTTGTATGCATTTATTGATATTGAAATGTCTGGCCTTTGGTGTAATGGTAACTTTTGCGCAAGTTCAGCTACTGGTTCATGTTTCTCTCTTTTTCCGGACTTGTGCGAGCGCATGAGTATTTGACTGGTTCTATAGTACACGGCAGTTCAAGGTGGTTTATGTGCACAAAGAGAATGGTCTCACTCAAACCAGCCAATAAAGCTTAAATATGTAATGACCTTGAAATTCAACTCATTCTTCCGTTTATGTTACTGGAATATATATATTTTGTAATATGATCATTAACTGGMTAAGAGATCGAGT
TARGET=431,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00444 Ps_contig_mira-and-tgicl-ass_franssen_4689
SEQUENCE=TTTCAGAATTAACAACAGAAAAGCTGAATTCAGATTTAACAGCAGAAGAACCATTGAGGAGCATAAGTATAAGTTGATTTAAGGGTGCATCAATGTTTTCTTTGTTTTGAAGTTGAGTGAGTGAGCTTTGTTTTGCTTTTGTTTATTGGAAATTAGAGTGATTAGGGTTTTAGACTGCTGTGGTTTAATATGATTGTTTTAATAAGAAATGTTTGAGTTTTTGTGTAGAAAATTATTATTGCATTTTATGTTAAAATATATACGTCGGTTTAGTTTTTGTTTTA
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0652
PRIMER_LEFT_PENALTY=0.629991
PRIMER_RIGHT_PENALTY=0.435201
PRIMER_LEFT_SEQUENCE=CAGAAGAACCATTGAGGAGCAT
PRIMER_RIGHT_SEQUENCE=AAACCACAGCAGTCTAAAACCC
PRIMER_LEFT=42,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.630
PRIMER_RIGHT_TM=59.565
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=1.0959
PRIMER_LEFT_1_PENALTY=0.660713
PRIMER_RIGHT_1_PENALTY=0.435201
PRIMER_LEFT_1_SEQUENCE=AACAGCAGAAGAACCATTGAGG
PRIMER_RIGHT_1_SEQUENCE=AAACCACAGCAGTCTAAAACCC
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.661
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=1.4181
PRIMER_LEFT_2_PENALTY=0.629991
PRIMER_RIGHT_2_PENALTY=0.788102
PRIMER_LEFT_2_SEQUENCE=CAGAAGAACCATTGAGGAGCAT
PRIMER_RIGHT_2_SEQUENCE=AACCACAGCAGTCTAAAACCCT
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=60.630
PRIMER_RIGHT_2_TM=59.212
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=1.4488
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=0.788102
PRIMER_LEFT_3_SEQUENCE=AACAGCAGAAGAACCATTGAGG
PRIMER_RIGHT_3_SEQUENCE=AACCACAGCAGTCTAAAACCCT
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=59.212
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=1.6154
PRIMER_LEFT_4_PENALTY=1.180219
PRIMER_RIGHT_4_PENALTY=0.435201
PRIMER_LEFT_4_SEQUENCE=ACAGCAGAAGAACCATTGAGGA
PRIMER_RIGHT_4_SEQUENCE=AAACCACAGCAGTCTAAAACCC
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=61.180
PRIMER_RIGHT_4_TM=59.565
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_00445 Ps_contig_mira-and-tgicl-ass_franssen_4699
SEQUENCE=TTCCAAGGCGGAAGAGCTTGGTTTACTCAGCTTATTGGAGAAATTCGCCACTGTTTCTCCTTCTGTTTTGGCTTCTCTTTCGCTTCCTGTGCTTGTTGCTGCTGTTGCCGCGGTTGTTCTGATTCCGGATGATTCTACTGCTCTGGTTGTAGTTCAGGCCGTCGTGGCGGCGGTTCTTGGTGTCGGCGCTGTTGGTTTGTTCGCTGGGTCGGTTGTGCTAGGTGGATTGCAAGAGGCTGATTGATTGGTTCAGTTATTTTAAACGTTTTTTACTGTTGGTTTGTTTTTATGTTAATTACATTGTAGTAATTACTTTACTTCTATTTGTTTTAATACTAAAATTAATTAATTTTTAATTTTAACTTAAAAGAAAAAAACT
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00446 Ps_contig_mira-and-tgicl-ass_franssen_4703
SEQUENCE=AAAGACTTTCTTATNTGCTGCTGATTTGTTTGTTTGGTTTTTGTTGGATTGTTCCTTCAGAATTAGAGCTAGTTTGAGTGTTGGGATAGTGTGGATGCGAATGCGCATTTCTTGCTTAGTTTGATAGATATATATGATATTTTATATGATTTTTCCTAATAATCTGGAGATTTATTTGAAGTTTTATGTTTACTCAAAAACAAGATTAAAGTTTATATTAACAGACCATACYAATAAATTTTTTATTGTTGTTTCG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00447 Ps_contig_mira-and-tgicl-ass_franssen_4707
SEQUENCE=GGGGGATGGATTGAGTTAAAGTGTGTGTACAAACAAAGTCAAGTCTAGAGCTTCTTTCTTTCTACATTAGCACCTATAGTTCTTTTCTCTGTGAAACTTCAAAACATGGAGAAGAAAATGAGTTGGACAGTTGGAGATGCTGTGGACTATAAAGGCTTCCCTGCTGATAGGTCCAAAACTGGTGGTTGGGTTCCAGCTGCTCTTATTTTAGGGATTGAAATTGTTGAGAGGCTCTCTACAATGGGGATTGCAGTGAACTTGGTGACATATCTGATGGGAGTCATGCATCTTCCAAGCTCAACAGCAGCCAATACCGTGACCGATTTCATGGGCACGTCGTTTCTCCTTTGTTTGCTTGGAGGTTTTCTAGCAGATTCCTTCCTTGGAAGATTCAAGACTATTGGAATCTTTGCTGCAATACAAACACTGGGTACTGCAGCATTAGCAATCTCAACAAAATTGCCACGGCTAAGTCCACCACCTTGTCACGCGGGCGAAATTTGCAAACCGGCTAATGGAATCCAAATGGGAATCTTATACTTGGCTCTATACCTTATTGCACTAGGAACCGGTGG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3728
PRIMER_LEFT_PENALTY=0.296177
PRIMER_RIGHT_PENALTY=0.076597
PRIMER_LEFT_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_SEQUENCE=GATGCATGACTCCCATCAGATA
PRIMER_LEFT=1,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.704
PRIMER_RIGHT_TM=59.923
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.4109
PRIMER_LEFT_1_PENALTY=0.296177
PRIMER_RIGHT_1_PENALTY=0.114715
PRIMER_LEFT_1_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_1_SEQUENCE=AGCAGGGAAGCCTTTATAGTCC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=164,22
PRIMER_LEFT_1_TM=59.704
PRIMER_RIGHT_1_TM=60.115
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4178
PRIMER_LEFT_2_PENALTY=0.296177
PRIMER_RIGHT_2_PENALTY=0.121635
PRIMER_LEFT_2_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_2_SEQUENCE=CCCTAAAATAAGAGCAGCTGGA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.704
PRIMER_RIGHT_2_TM=59.878
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.4401
PRIMER_LEFT_3_PENALTY=0.296177
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_3_SEQUENCE=CAACCACCAGTTTTGGACCTAT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=187,22
PRIMER_LEFT_3_TM=59.704
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.4828
PRIMER_LEFT_4_PENALTY=0.296177
PRIMER_RIGHT_4_PENALTY=0.186600
PRIMER_LEFT_4_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_4_SEQUENCE=TCCCATCAGATATGTCACCAAG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=278,22
PRIMER_LEFT_4_TM=59.704
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_00448 Ps_contig_mira-and-tgicl-ass_franssen_4720
SEQUENCE=GATTTGGAGTTGAATTAGATCTTCCGTGATTGTGAGAGTGATTCATTCTTAGTATACACTCCCACTGCAAAATACAAAGAAAGAAGAAGAAGGATCTGTTTGGATCCAACGATGAAGTATTTTGGGTTTATTTTTTGTCTTGTGTTATTGATCCACTCTTGCCGCCGCCGCCGAACCATTAACTGTGGATGGGAAAGGTGTTGATACTCGACGAGTCCAATTTCGATTCCCGCCTATTTC
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1622
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT=56,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2277
PRIMER_LEFT_1_PENALTY=0.118777
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_1_SEQUENCE=ACCCAAAATACTTCATCGTTGG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=126,22
PRIMER_LEFT_1_TM=60.119
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.2486
PRIMER_LEFT_2_PENALTY=0.118777
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_2_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.119
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.2739
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_3_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.3603
PRIMER_LEFT_4_PENALTY=0.118777
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_4_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=60.119
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_00449 Ps_contig_mira-and-tgicl-ass_franssen_4720
SEQUENCE=GATTTGGAGTTGAATTAGATCTTCCGTGATTGTGAGAGTGATTCATTCTTAGTATACACTCCCACTGCAAAATACAAAGAAAGAAGAAGAAGGATCTGTTTGGATCCAACGATGAAGTATTTTGGGTTTATTTTTTGTCTTGTGTTATTGATCCACTCTTGCCGCCGCCGCCGAACCATTAACTGTGGATGGGAAAGGTGTTGATACTCGACGAGTCCAATTTCGATTCCCGCCTATTTC
TARGET=161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1622
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT=56,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2387
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.129818
PRIMER_LEFT_1_SEQUENCE=CCAACGATGAAGTATTTTGGGT
PRIMER_RIGHT_1_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=0.2486
PRIMER_LEFT_2_PENALTY=0.118777
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_2_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.119
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.2739
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_3_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.3505
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=CCAACGATGAAGTATTTTGGGT
PRIMER_RIGHT_4_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_4=105,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00450 Ps_contig_mira-and-tgicl-ass_franssen_4724
SEQUENCE=AAAAATTWACGATAAGCTTATGGATCTTAATATTATATATATACTACTGACCAATGTTTTTGGGTGAATACACCATTTACAAAATCAATTTAAAAATAAAACAATATACACTGGCAAGTCTTTTTAAAATAAAACTCTAAATTCCACAACATTACCTCGTATGCAATTATAAATCATATTTTAGAAGAAAATATCTGATTGTTTTGACTGGGCTTTAAGATCCTTCAATTTCTTCAATATGTTTGTGAAGTAGTCCTGCAGATACTTTTCCAATGTAATTATATCTTTTGTATCCACACCTAGCAGATTGTATGTCTCCCCCATCGGAACAGAGAAAATTGTGTCACTCGTAAGTACCTCTGAAAATGCCAATCTGTCAGCAACATCATTTGTCCACTCAAAAAACCTAGTTAACTGGCGAGTAAATCTTAAAACAGAGACAGGAACTGTAGTTACATTAGCATCTTGACCAGCAAGCCTCTCACACAATGTTATCACCTCTTGGGTTGTCCATGCACGAGGACCGGCAAAAGTAAGAAGTTTACCATTGATTTTTTCATTTCGCAATGCTATGAAAGTCAAGCGAGCTATATCCTGAGTATCCATGTAAGCAATTCTGGTGGGAGCATCAGTTCCCCAAACAGACTTTTCTTCCAGGATAGGAACTGCATACTGGCCTATAAGACCTTGCATGAAACCACATAGGCGAATGACAACGTGATTGAGTCCGGAATCACGGAGGAAGTTCTCGGTGCAATACTTGATCTCCATGAGTGGAACCTCGGGATGTTTGTCGCAGTTATGGATGGAATAGAAAACATATTTCTGAATTCCCATAGCTTTGGCACATTGTATAAGAGCCACTTTACCTTCCCAGTCTACAGTTTTGATGGGCTCTTCAGGACGGCCAGTGGCGCAGTCAATAATAGTATGAACGCCAACCAAAGTAGCGGGAATAGTCTCTGGTTTACTAAGATCGCCATTGACAACAGTTGCTCCCCAATCACGAAGGAAGTCAGCAGGAGCGGGCCTCGGCCTTACAAGGCACCTGACGTCGTATCCTTCGTCGAGTGCTCTTCTCACAATCTGCCTTCCGAGAGTGCCGGTGGCTCCCACAACAAGTATGTTGGTTGACCTAACAGGTGTCCCGGTCCCAAGATTCACGGCGGATCTATCACCAACATCTCCACTAATACTACATTTCACACCACCGACTCTCCTATTACTACTTCCATAAACGTCAAGGTGAAATGCATGTCTCCTCGTGGAGGGTGCTAGTGCTACACCCCATAGTCTCACCCTAGAACCAGTAGCAGTAGAAGTGGGTAGCCTCAAACCCGCCATTTTCATATCACCGGGAAATGCTTCGTTCTTCTCTTTCTAATTCTCATTCAATCCGATACTCATGATCGTAATTTCGCCACGTCTTCCATTGCTACCACACACCGCC
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00451 Ps_contig_mira-and-tgicl-ass_franssen_4725
SEQUENCE=AAGATATAATTACATTGGAAAAGTATCTGTCTAGGACTACTTCACAAACATATTGAAGAAATTGAAGGATCTTAAAGCCCAGACAAAACAATCAGATATTTTCTTCTAAAATATGATTTATAATTGCATACGGGGTGATGTTGTGGAATTTAGAGTTTTATTTTAAAAAGCTTGCCAGTGTATATTGTTTTATTTTAAATTGATTTTGTAAATGGTGTATTCACCAAAANTTGGTCAGTATGTATATATATAATATTMAGTWCCWTAAGACTT
TARGET=243,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00452 Ps_contig_mira-and-tgicl-ass_franssen_4730
SEQUENCE=AGGTTAGCCAGCAAAGAGGATTCCCAGGTGGCAGGTGACGATTGAACAATTAAGAACCAAACACAGCTCCTCCAAAAGCATCTTGAAAACTAGAAACACGCAAAGAATGCATGTTTTTGGGGATTCGGTTGTGAAGAACAAGAGGGTTTGGAAATCGAGTTATTAAATTGGGTTTTGGAGAGAGAGAAATGGGTTTGCGTGGGAAGTGAAAAGGAAGAGAAAGATACGTGATYGAGTGAAGAGTTAATTGCGCCATAATGTGTCTGTCGATGTCAACGTGAAGGGAAAAAAGATGAGAATGAAAAGAAA
TARGET=178,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00453 Ps_contig_mira-and-tgicl-ass_franssen_4757
SEQUENCE=GATAGAGAGTGTGACTAAAGAATCSGTGTTCGAATCGAAGAGAGCGCAAACTAAACACGGAGCTATTTATTTATTCCTTCTTTAACGCCCTTTTGACTTCACACCCTTCGCATTCTCCGCCACCTTCTCAACCCTCTCTTCTCTTTTTCACTCTCTTTCTCTTCGTCTCTCCACACAAACAACACCACCAACCTCCATGGCGATCAATATGCAAGCATCACACTCTGTGCTCTCTCTCCGTCCTCAACTCAATCAGCGACTGGCTGTCACTTCAACCGCCGGCCAAAGGTCTTCCTTAATTTCAAGCTATGGGAGATGGAATGTGTCTTTTAGCCCAATGTCACGCCCAATGCGCCTTACTGTTACCTGTGCTGCTAAACCACAGACAGTGGAAAAAGTGAGCCAGATAGTGAAGAAGCAGCTGGCTTTATCAGATGACTCTAATGTGACCGGCGAATCAAAATTTGCAGCTCTTGGAGCTGATTCGCTTGACACGGTTGAGATTGTGATGGGACTCGAGGAAGAGTTTGGCATCAGCGTTGAAGAGGATAGTGCTCAGAGCATCACAACAGTTCAAGAAGCTGCTGATCTTATCGAGGATATCCTGAGTAAACAGAGTCCTTAGACGAAAAATGTTGCAGATGTAAAACGCCTCTGAAAGACATGCATAGTTCTTATTCATACTGTTTTTTAGAATGTGGTGGTCAAGTCTCTAGTGTTTTATCTGCATAGTTTGCTACTGTCTTGTTTCGTAGGTCTTAAAGAGCAAATTAGCGAGCTACTTGTGTTTTGGAGAAAATGGCTGTGTAGTCCGTTCTTACTTTCTTTGCTCATTAAGGGCTTATCAAATTGTTTTAGTTACTTTAAGTTGTTGAGCATGCAATCCATAAATTTGCTGCATAATAATGAATAAGAGACTAAGTTAAGGGTAAATAAAAAATTTAAAAAAAAAACG
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00454 Ps_contig_mira-and-tgicl-ass_franssen_4776
SEQUENCE=AAAAGTAAAAAGAAGAAAGTAAAAGTAAAGTAGTGAATTCATCAAGAACAACCGGTTAATATTCCTAATTGGTCAAAGATTTATGGTCATGATGATGATAAGGTAAACAAGAGTGTTAAGAATGTTTCAAGGTATGGTAATGATAATTATGGTTACTATGATGATCATGAAGGTGGTGATGATCATGAAGTTTTGAACCATGGCGAAAGAGGTAGCGATGATGATGATGATGATGGTGAAGATGAAGATGAAGATGATGATGGTGATGGTGAGTATGGTACTAGGCTGCCACCTCATGAGATAATTGCGAGAAGGCTTGCAAGGAGTCAGATTTCTTCATTTTCTGTTTTTGAAGGTGTTGGTAGGACACTTAAGGGTAGGGATCTTAGCAAAGTGAGGAACTCTGTCCTCATAAAGACTGGTTTTCTTGAATCATTATGATTAAGGGTTTTTCAATTGATCAAATTTGTGAGGTTTTAAATTACGGTTGCGAACTTTTGTATTGCATTAAATTTTGAGAATTTGAAATTGCAGTTGTAATATAGTTGCAAGGACTTCTCAATTA
TARGET=217,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2808
PRIMER_LEFT_PENALTY=0.203457
PRIMER_RIGHT_PENALTY=0.077340
PRIMER_LEFT_SEQUENCE=GATGATCATGAAGGTGGTGATG
PRIMER_RIGHT_SEQUENCE=GGACAGAGTTCCTCACTTTGCT
PRIMER_LEFT=159,22
PRIMER_RIGHT=408,22
PRIMER_LEFT_TM=60.203
PRIMER_RIGHT_TM=59.923
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.2808
PRIMER_LEFT_1_PENALTY=0.203457
PRIMER_RIGHT_1_PENALTY=0.077340
PRIMER_LEFT_1_SEQUENCE=GATGATCATGAAGGTGGTGATG
PRIMER_RIGHT_1_SEQUENCE=AGGACAGAGTTCCTCACTTTGC
PRIMER_LEFT_1=159,22
PRIMER_RIGHT_1=409,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=59.923
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.2808
PRIMER_LEFT_2_PENALTY=0.203457
PRIMER_RIGHT_2_PENALTY=0.077340
PRIMER_LEFT_2_SEQUENCE=TGATGATCATGAAGGTGGTGAT
PRIMER_RIGHT_2_SEQUENCE=GGACAGAGTTCCTCACTTTGCT
PRIMER_LEFT_2=158,22
PRIMER_RIGHT_2=408,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.923
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.2808
PRIMER_LEFT_3_PENALTY=0.203457
PRIMER_RIGHT_3_PENALTY=0.077340
PRIMER_LEFT_3_SEQUENCE=ATGATCATGAAGGTGGTGATGA
PRIMER_RIGHT_3_SEQUENCE=GGACAGAGTTCCTCACTTTGCT
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=408,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=59.923
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.2808
PRIMER_LEFT_4_PENALTY=0.203457
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_LEFT_4_SEQUENCE=ATGATCATGAAGGTGGTGATGA
PRIMER_RIGHT_4_SEQUENCE=AGGACAGAGTTCCTCACTTTGC
PRIMER_LEFT_4=160,22
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=60.203
PRIMER_RIGHT_4_TM=59.923
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_00455 Ps_contig_mira-and-tgicl-ass_franssen_4788
SEQUENCE=AGTGGTACATATTGTCCGAAGAACCATGTGKMDGGGAAGGTTCATTCATTTTGTTCCTCCAACGTATGCTTCTCACTAACATCATCAACAAACCAGTGACAGAAAATGGAATAAAAAATAACAAGAAAAAAACACAGGAAGAATCATAATGCAAATGGCACTTTTGTTAAGAGGTGGAGCACTTGGAGATTCTACCTTTCGTTTGTGTTCTTTAACTTCAACCTCATGTTCTTTACATGTCTCCCAAAATGTTGTCATACCGAACTCATCGTCATCACCAATACTACCTTTGATTGCAAGTAGATTTAAAACGGTAAATAGAAACAAGATAATTTGCTCTGCAGTTCAAGAATCATCTACTACTAGTACATCAGCTACTGCTGAAACAAAGGGGGAGGTAAAGGCAGCAGCAGCAGCAGCTCCAAAAGCAGCAACAGGGAAGAAGGCTCCACCTGCTAAAGCTCCGGTTAAGCCTTTACCTCAGATGATGGAAGAGGATGTGATTCCTTCGTTGAAAACAATTTTGGAATCCAATAAGGATCTCTCTGATATTGAGTTGGTTTTTCAGGACAATAAGTTGGAAGGTTCATTTCTGAAAAAAGGCAATCCCTATTCATTTTGGGCCTTTTTTCCCACAGGAATTACTGGTCCAAAGGGGTTTTCACTGTCATCTTATAACTCAGGAGCAAGCTACGGTTGAACCGATTCTCATTGATGAGAAGAAATCNCTTCAAAACACATAGTTNTTTTGGGTTAGAAAAAACGGTTTTGGGCGAGACGAACGAGGGGGGTAATTTATTCCTGTAGTGGAAAG
TARGET=404,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00456 Ps_contig_mira-and-tgicl-ass_franssen_4811
SEQUENCE=AGGCATGGATTTAGGGTTTGACCACGAATAGAAAGATGATTTTCTTGAAAATTTGGATGCAATTAGAACCCTTCTTCTCTTGTTAAAAGGACTCTCTTGCTTCCGCAACTCGGTTAAATTGCTTACTTGTGATAGATCTGTGAAGGTATTTTTGACTTTTTCCTTACAAAAATGACTCGATAACTCCCCTCTAATCACAAGATTAATACAAAAATTAGAAAAATTAAAACAAAAACAAGTAGAGAGAAGAAAGACAAAGAGAGAAGAGAGAGAGAGAAGAAGAGAACTAACG
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00457 Ps_contig_mira-and-tgicl-ass_franssen_4813
SEQUENCE=ACCTTTCKTCKSRKYCTYCRTYTGGTTCGTCTCCATAACCTTGCCCCGAACCCGAACCATAGCACCCGACCCAAACTCCTAACCTAACCTAACCTAACACCGTTACAGTTATGGGAACGGAGAGTTATACCTACATGGGACGGAGTTTCAACGAATTCACCATTAACGACGATTCTACTTCCTCCGCTTTCAGCGACTGTAACAGTGATAGATCCGGCGAATTCGCCACCACTTCTTCTCAATCCCCGACGACTCTATTATCGCCTGTGCTT
TARGET=78,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00458 Ps_contig_mira-and-tgicl-ass_franssen_4814
SEQUENCE=TGTTGATATTTGGGTGGAAAACTTTAGTCCTAAATGTCTAACCTTGGGTGGCTTGAATGGATAATCAGGAGGAAAATGAATGGTAACTAGAAAAACACCTCCAGCATAAGGGCTATCAGGGGGACCCATTATAGTTGCTTGCCAATGAAACATATCTTCTGCTACAGGGCCAGCACTGCATGAAGTTGGAGGATCTTTCTGAAGGTCCTTGAGTTCCTTCAGGATCCGCTTCGAGGCCATGGAAATACAGAACAAGATGTGAGAGAGAGAGAGAGAGAGGAGGTASAMCCTTAAACGTTTTAAACGTTAAAAAACGTAAAAGGAAAAAGGTAAAAAGGTAAAGGAAAAAAGAAAACGAACTAAAACGG
TARGET=261,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00459 Ps_contig_mira-and-tgicl-ass_franssen_4817
SEQUENCE=GGAAAACAGCCGCCAATAATAAACCAAACAGAAAAAACGCTGGGGGATTTTTTTTCATTTCTCATTCTCGTTCGTTCATTCCTTAGCGTTTTTTGTTGAATCCAATCGAAGAAGAAGAAGATGGGGAAAAATATTGTGCCGGTGAAATCTGTTGTGTATGCTCTTTCTCCTTTCCAGCAGAAAATCATGAGCGGTTTGTGGAAGGATTTGCCAAACCAAAATTCATCACAAGGTCTCCGAAAAATTGGATCAGCGCTATTCTCTTGTTACTCCCGTCGTCGCACTTACTCTATGCTA
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3415
PRIMER_LEFT_PENALTY=0.328094
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_SEQUENCE=GCTGGAAAGGAGAAAGAGCATA
PRIMER_LEFT=69,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=59.672
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.4514
PRIMER_LEFT_1_PENALTY=0.328094
PRIMER_RIGHT_1_PENALTY=0.123278
PRIMER_LEFT_1_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_1_SEQUENCE=TTCTGCTGGAAAGGAGAAAGAG
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.672
PRIMER_RIGHT_1_TM=60.123
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.5852
PRIMER_LEFT_2_PENALTY=0.571751
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=CCGCCAATAATAAACCAAACAG
PRIMER_RIGHT_2_SEQUENCE=GCTGGAAAGGAGAAAGAGCATA
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=60.572
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.5970
PRIMER_LEFT_3_PENALTY=0.328094
PRIMER_RIGHT_3_PENALTY=0.268895
PRIMER_LEFT_3_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_3_SEQUENCE=CGGGAGTAACAAGAGAATAGCG
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=59.672
PRIMER_RIGHT_3_TM=60.269
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=9.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.6011
PRIMER_LEFT_4_PENALTY=0.328094
PRIMER_RIGHT_4_PENALTY=0.273036
PRIMER_LEFT_4_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_4_SEQUENCE=GAGAATAGCGCTGATCCAATTT
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=59.672
PRIMER_RIGHT_4_TM=59.727
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_00460 Ps_contig_mira-and-tgicl-ass_franssen_4826
SEQUENCE=CGYGTAGTAATAAATCACATGACCATATAGAGAATCAAAACTAACAAGAAAGATATTGTCACAACGACCCTGCAAGCATTCTTTAAATTATCGTGTGATAGACCATGGCAAAATAATTGATCATAAGAGTCTCAGGTTTCTTAAATAAATGACAAATACACCTGTTACTTATGCACTATTGGCTATCGTAAGAAAGGTCATTGAAATTGCGTAAAGTTAGGGGGTTGACTCAAGACTGATAAAATTGTGTGCATGGCATGTCCATGCTGCAGCAGCAGCAGCAGCAGCGCCTGTTGAGAGTTGAACACAATGAAGGGATACGGAGATACCCGTATAGTTTTATCTCACTTTCTTCTCTTTAGTAGCCTGAGGCTTTTGGTACATCACTATCTGACTTAAGATGGTAAAATTTGTCGCAACACCAATTGCATAGCCCAGTAGAACACTTTGGGGGGCATTTTCTTGGATGGTGGTGAATACTCTAACCATTGAACCTCCAAAGTTCATAAAAGAAGTTAAAAAGCTGAGCTCCCCTGTACTTTTATTAGAAAAGTTTTGCCATACTTGTGGGACCCTTGCACACAGAAATATTAAATGCTGGGATGCATACAAGGCCTCAAAGAGGATAGGATCAATTTTACCAGCTAAGACTGTGGGTGCTACAGCGCAATATATCAATGGCCGAATCCATGTGATTGTACTAATAGGTTGAGAATAGTAGTAGATTATGGCAACCAATACAAAAGCTTGGATCAAAAGAAACAACAATTCTCCATAAGCTGAAAAAGGAAGGCCTTTGTGAAGAGAGTAAGCCAATGCTATGGTATACCCAACAACTTCAAGCTCGAACGCTAACATGCTAAGGCCTCTCACACTTTTATGCTTCAAAATTTTCAAAATCTGAGGGAGTTTGACGGTGGTGGAAGCGGCGACGATGGCGTAACCGAGGAGTTTGGAAATGAGAGGAAGCAAGCAATCTTTGTCAGGGAAGTTTCCGTTACGAAGAGATCCAACGGCACAACTCAAATCAATCCCTAGATATTCCATCGTCTTCTCCGATCTCAATCTAAGGGTTTCTTTCAATGCAAAAAATCGCTTCTCTGGTTTGTTGTTGTTGTTGCCGTCAATGTTTACTCAAACTGAACTTTCTAAAATTCTCTTTTTTTCGTTGAATCMTCCCCGA
TARGET=269,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00461 Ps_contig_mira-and-tgicl-ass_franssen_4826
SEQUENCE=CGYGTAGTAATAAATCACATGACCATATAGAGAATCAAAACTAACAAGAAAGATATTGTCACAACGACCCTGCAAGCATTCTTTAAATTATCGTGTGATAGACCATGGCAAAATAATTGATCATAAGAGTCTCAGGTTTCTTAAATAAATGACAAATACACCTGTTACTTATGCACTATTGGCTATCGTAAGAAAGGTCATTGAAATTGCGTAAAGTTAGGGGGTTGACTCAAGACTGATAAAATTGTGTGCATGGCATGTCCATGCTGCAGCAGCAGCAGCAGCAGCGCCTGTTGAGAGTTGAACACAATGAAGGGATACGGAGATACCCGTATAGTTTTATCTCACTTTCTTCTCTTTAGTAGCCTGAGGCTTTTGGTACATCACTATCTGACTTAAGATGGTAAAATTTGTCGCAACACCAATTGCATAGCCCAGTAGAACACTTTGGGGGGCATTTTCTTGGATGGTGGTGAATACTCTAACCATTGAACCTCCAAAGTTCATAAAAGAAGTTAAAAAGCTGAGCTCCCCTGTACTTTTATTAGAAAAGTTTTGCCATACTTGTGGGACCCTTGCACACAGAAATATTAAATGCTGGGATGCATACAAGGCCTCAAAGAGGATAGGATCAATTTTACCAGCTAAGACTGTGGGTGCTACAGCGCAATATATCAATGGCCGAATCCATGTGATTGTACTAATAGGTTGAGAATAGTAGTAGATTATGGCAACCAATACAAAAGCTTGGATCAAAAGAAACAACAATTCTCCATAAGCTGAAAAAGGAAGGCCTTTGTGAAGAGAGTAAGCCAATGCTATGGTATACCCAACAACTTCAAGCTCGAACGCTAACATGCTAAGGCCTCTCACACTTTTATGCTTCAAAATTTTCAAAATCTGAGGGAGTTTGACGGTGGTGGAAGCGGCGACGATGGCGTAACCGAGGAGTTTGGAAATGAGAGGAAGCAAGCAATCTTTGTCAGGGAAGTTTCCGTTACGAAGAGATCCAACGGCACAACTCAAATCAATCCCTAGATATTCCATCGTCTTCTCCGATCTCAATCTAAGGGTTTCTTTCAATGCAAAAAATCGCTTCTCTGGTTTGTTGTTGTTGTTGCCGTCAATGTTTACTCAAACTGAACTTTCTAAAATTCTCTTTTTTTCGTTGAATCMTCCCCGA
TARGET=1106,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00462 Ps_contig_mira-and-tgicl-ass_franssen_4837
SEQUENCE=GAATCTCTCTTGCTCTTTCATCGCTTCTCTTCAATTTTATTTCAAAGTTGAAAGAAGCTATCATACTCTCATGGCGTCCGAAAATTCCAAACCAGAGGTCTCGTTAGAGAGTTCAAAGGTAGAAACAATAAGCAAAGCTGTAGATTCTCTTCTCAAATGGCACAAATACGACGTTTAAGAAAACCGAAAAACCTTAAACTTTTACGAAGAAGAAGAATTCATCTATCTGGTATTAACCCTAAAGAAGATTCCTAACAAATCGGCCGTGTTAACCCTCA
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7510
PRIMER_LEFT_PENALTY=0.003166
PRIMER_RIGHT_PENALTY=0.747796
PRIMER_LEFT_SEQUENCE=TCTCAAATGGCACAAATACGAC
PRIMER_RIGHT_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT=150,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=60.748
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.8715
PRIMER_LEFT_1_PENALTY=0.123706
PRIMER_RIGHT_1_PENALTY=0.747796
PRIMER_LEFT_1_SEQUENCE=ATCTCTCTTGCTCTTTCATCGC
PRIMER_RIGHT_1_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.124
PRIMER_RIGHT_1_TM=60.748
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.8000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=1.0098
PRIMER_LEFT_2_PENALTY=0.003166
PRIMER_RIGHT_2_PENALTY=1.006651
PRIMER_LEFT_2_SEQUENCE=TCTCAAATGGCACAAATACGAC
PRIMER_RIGHT_2_SEQUENCE=TTAACACGGCCGATTTGTTAG
PRIMER_LEFT_2=150,22
PRIMER_RIGHT_2=271,21
PRIMER_LEFT_2_TM=60.003
PRIMER_RIGHT_2_TM=60.007
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=1.0302
PRIMER_LEFT_3_PENALTY=0.282356
PRIMER_RIGHT_3_PENALTY=0.747796
PRIMER_LEFT_3_SEQUENCE=CTCTCTTGCTCTTTCATCGCTT
PRIMER_RIGHT_3_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=272,22
PRIMER_LEFT_3_TM=60.282
PRIMER_RIGHT_3_TM=60.748
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=1.0302
PRIMER_LEFT_4_PENALTY=0.282356
PRIMER_RIGHT_4_PENALTY=0.747796
PRIMER_LEFT_4_SEQUENCE=CTTGCTCTTTCATCGCTTCTCT
PRIMER_RIGHT_4_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=60.282
PRIMER_RIGHT_4_TM=60.748
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_00463 Ps_contig_mira-and-tgicl-ass_franssen_4838
SEQUENCE=RGATATGAATAATCGACGCGATTTATTTGCTCATGACACGAGCTTAGCTCATTCTTTTCTGTAGAATCATACACCAAGAGTCTCATCACTTATTTTCTTCATATAATACAGAAATCAATTAACTTATTTCCTCAAAATACATACTCCAACTATTTGCTAATTTCTGAACTTAATACATCAATTATAACTTACATGGTAAAAACGTCACGCAGTAAAGGAAAATTCAATTGAAACGCGCCACCAATATTGAATGACTCACCAGAGCTGAAACCATATTCTCTCAAGCAGCCAGCCTGTGATTTGTACTCTACTTGAGGAATTACATTTGTTTGTACAAAATGTTATTCGCCATCACTCAAGATGGGAACTATGAGGGGGTTCATTTGTGCCAGATATACAATTGCCCACATCATCCAGCAGCATATTGTTGAAGTAAGGACCAAAGTGAGGTGAAATAAATTAGCAGAAGGTCCTTTGTTGAAGCAAATTCTTGTAGAAAGGCATGCGATGATTCCGACGACAAGGAAGATAAGCGAGGTGACAACGAAACCCATATCGATCTCTGAAACGGTTTGTCGTTTATTGTATGAATGAATGATGGGAAGGTGAAGCTCCCAGGTGGGGTGTGTATTTTATTTTTTATTATTCTTATTTMAAATTTTGGTATTTTTGTGGTACCTTATACTC
TARGET=587,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00464 Ps_contig_mira-and-tgicl-ass_franssen_4839
SEQUENCE=GATCATTCATTCATACAATAAACGACAAACCGTTTCAGAGGTAACCTAATTCTCACGTCGTTTTGATTCTTCCCTCACTTTCTCGCAAATCAAATCAAAAACCGTAACTTTAACAGAACCATTTTCTTTCAATTTGTGTAATTCAGATCGATATGGGTTTCGTTGTCACCTCGCTTATCTTCCTTGTCGTCGGAATCATCGCATGCCTTTCTACAAGAATTTGCTTCAACAAAGGACCTT
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00465 Ps_contig_mira-and-tgicl-ass_franssen_4841
SEQUENCE=ACGGGGGAGGAAATGNGATTGTTGAAGAAAAAGAAAGACTAGAGATGGTGCAAGATTTCTTCATTAGTTACTATGAGATGTTAAGATTTATGATTATGATGATGATGATAGTTGAAAAGTGGTTTGAAATGACGTGTTATAAGAGAAACCACATTTCTTAGGATCTTCTTTAGATTTCCCTTTAAAAATTATAACAATGTTGTTTTGGTGTTTTAAACTTATTTTTTATTTTCTTTGATCTTTGTGTTAGAGAGTTCATTTATTGTATGTGCGAGGTTTTGTTGAATGAAGATGAATATCTCTCCTTTATTTTCTCATAM
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00466 Ps_contig_mira-and-tgicl-ass_franssen_4892
SEQUENCE=ACATGCCTGCTCAAACCATGACCCCTGAGCTGCAGAGAAATCTGAAGTTACTAAAGTTGAGGGATGCCATTGATCCAAAACGGCACTACAAGAAGGGTGATTCAAAATCAAAGAGTCTTCCAAAATACTTTCAGATCGGAACAGTTGTAGGTTCTCCGCTGGACTACTTCTCAGGCAGATTAACAAAGAAAGAAAGGAAAGAAACGTTTGCAGATGAGTTGCTTGCTGATCAAAAAGATTCAGCTTACAGGAAGCGTAAGGTTCGAGAAATCGAAGAGCAGAGGCAACCAGCTGGGAATGAAAAGTGGAAGATCAGAGGCCAGAGTTCTCGGAAACGTGCAAAAGAAAGGAGGAAGTTCTGAATTTTACAGACTTGTTGGTTGGAGTTTATTGCATTCAGTTTTGTTTACAAGTCTGGAAATTAATGCAATGGAACAATTTATAATGGTTATATTTAGTCGATTTATATAATAAGTCTAGTCAGTGTTTGGAGTCTCATTTTTGTATTATGGAAATTTATGGTCGTAATTTCTTTCAACTTTAGCATTTGTATCATAAGAATTAGTTATTGTTGTTTAAGCTCATTTTTAAAAAAATGAAGAAGTGAAACAATTTAAGAAGCTGAGTACGCATATTAT
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0267
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_SEQUENCE=TTGATCAGCAAGCAACTCATCT
PRIMER_LEFT=81,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.2536
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.249232
PRIMER_LEFT_1_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_1_SEQUENCE=CTTCGATTTCTCGAACCTTACG
PRIMER_LEFT_1=81,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.3034
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.299089
PRIMER_LEFT_2_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_2_SEQUENCE=GATCTTCCACTTTTCATTCCCA
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.3034
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.299089
PRIMER_LEFT_3_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_3_SEQUENCE=TGATCTTCCACTTTTCATTCCC
PRIMER_LEFT_3=81,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.299
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.3991
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.394711
PRIMER_LEFT_4_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_4_SEQUENCE=TTTGATCAGCAAGCAACTCATC
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_00467 Ps_contig_mira-and-tgicl-ass_franssen_4921
SEQUENCE=AACACGATTCTGACACTTATGATTATGTTTAATTTATTTATTTTTCAAATCATCATTGATATCAATGTGTCAGAATCGAAGTCGTGTCTGGTGTCCGTCATGATATCTATTCAATAAAAAAAAAGTATTACATGTTAGT
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00468 Ps_contig_mira-and-tgicl-ass_franssen_4923
SEQUENCE=TTTTTTTGTATGATTTGAAAAAATAAATAAATTAAACATAATCATAAGTGTCAGAATCGTGTTGATATCTGACGTTGACACGAACACACCCTTTTTTCCCT
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00469 Ps_contig_mira-and-tgicl-ass_franssen_4925
SEQUENCE=ACACACANTANATTNTCTAATAATAATAACACATAATTGGTCAACTCCATACCAGGANCTTTAAGAAATAAACAAATTACGTATCTTCTTGAACTGGATGTGGTTGTCTCAAATTTGTATTATTATTRTTAGAGAAATRTAACATGTTGGTGTTGAAACTTGTGACACATGAATTAAGTTTCTTCTATTTTTTTCATTATGTTTATAAAATATTTTTAGAAAACACCA
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00470 Ps_contig_mira-and-tgicl-ass_franssen_4927
SEQUENCE=CTCTCTCTCTCTATAAGATACGCAGCATGCAACAGAAGAAGGGAAGTGTAAATTTTCTTGCACTGATTCAGATGGATATATGAAGATAGAAGTGTCCTGCTTCTACTTGTGTATTTTTTTTTTTCAATTCATTTGCATGATTTGTCTTCTGTCTGGCTATAAAGGTCATTTGAAACTTCATATAGGGTCATGCGATGGGCCTAGACAAATTTGAGGTTAACTTTTCTTGCTTTCTTTTGAAGAAAACTAGNGGTTCTGTCTTTTTGTAGTTGGTTTCTATTTCA
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00471 Ps_contig_mira-and-tgicl-ass_franssen_4963
SEQUENCE=CGAGAGCAAACATCATTTTATTTGKTCTTTGATGAGATCCAACTTTTCATTATTGTATGTTTAAGTTCACACACAATAAAGGTGCAATGCAAAGGCTTGTATATGTGTGTAAATTAAAACTCATACAGGGATAAATACACTGGGGAGCGACTGTATTCAGTATGAAATTAACCCATATCCCGGGATGGGAAACGGTGGGATCTAAGTCAAAAGTGGAAGAACAATATATAATACCACTTCCCATATATTTCTAACTATTTGTTACATTTTCAATTGAAGGAGAAACAACGCTTGTTGTCATATCCAATTTGAGCGCATGCCTTGTAATCTGGTTTGTTTGTTTTCTCAATCTAACATTATCAACGAACAAATCTGCTATGATCTTTCTCATTTCATCACCACAGATTGCACCATCTTGTATGACATTGGTTTTTTCCCCGATAAAAGCTGCATTTCCGTAGTCCTTCTCTAAGTTTTCTACCTCTGCTAGTAGGATGTTGATTTGGTCATCAGATGTCTTTAATAGATTAAAAGCATCAGTTGATGTTGATGAATGCAAGACTGTCCTATTCTCGTCATTGAAAGGAAGATCGACATTGCATTCTTGAAGCTGACTTAAAAAGAAGGCCACATTTCTCAAGCAATTCTCTAGAAGCTGTTTCTGCTTGTTTCCCTTTTCTCTCTTTCATGAAGTTAGTTAGTTTTCCTCCGCTTTCTTAACG
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00472 Ps_contig_mira-and-tgicl-ass_franssen_4963
SEQUENCE=CGAGAGCAAACATCATTTTATTTGKTCTTTGATGAGATCCAACTTTTCATTATTGTATGTTTAAGTTCACACACAATAAAGGTGCAATGCAAAGGCTTGTATATGTGTGTAAATTAAAACTCATACAGGGATAAATACACTGGGGAGCGACTGTATTCAGTATGAAATTAACCCATATCCCGGGATGGGAAACGGTGGGATCTAAGTCAAAAGTGGAAGAACAATATATAATACCACTTCCCATATATTTCTAACTATTTGTTACATTTTCAATTGAAGGAGAAACAACGCTTGTTGTCATATCCAATTTGAGCGCATGCCTTGTAATCTGGTTTGTTTGTTTTCTCAATCTAACATTATCAACGAACAAATCTGCTATGATCTTTCTCATTTCATCACCACAGATTGCACCATCTTGTATGACATTGGTTTTTTCCCCGATAAAAGCTGCATTTCCGTAGTCCTTCTCTAAGTTTTCTACCTCTGCTAGTAGGATGTTGATTTGGTCATCAGATGTCTTTAATAGATTAAAAGCATCAGTTGATGTTGATGAATGCAAGACTGTCCTATTCTCGTCATTGAAAGGAAGATCGACATTGCATTCTTGAAGCTGACTTAAAAAGAAGGCCACATTTCTCAAGCAATTCTCTAGAAGCTGTTTCTGCTTGTTTCCCTTTTCTCTCTTTCATGAAGTTAGTTAGTTTTCCTCCGCTTTCTTAACG
TARGET=692,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00473 Ps_contig_mira-and-tgicl-ass_franssen_4987
SEQUENCE=AAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAAGCACAGAGGGGGTGAGGATTATCATGGAGAAAGAAAGCGCAGAAGGGATGATGATGATCATGTGCATGGAAGAAAGAGCATTANAGAAGGNTGATGATGATGGAAGAAAAGAAAGTACAGGAAGGGAGTTAGTTT
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1829
PRIMER_LEFT_PENALTY=0.109273
PRIMER_RIGHT_PENALTY=0.073614
PRIMER_LEFT_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_SEQUENCE=TCTTCCATCATCATCANCCTTC
PRIMER_LEFT=32,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.4889
PRIMER_LEFT_1_PENALTY=0.415247
PRIMER_RIGHT_1_PENALTY=0.073614
PRIMER_LEFT_1_SEQUENCE=GTGAGGAAAGAAAGCACAGAGG
PRIMER_RIGHT_1_SEQUENCE=TCTTCCATCATCATCANCCTTC
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=60.415
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.7085
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.634599
PRIMER_LEFT_2_SEQUENCE=AGAACTCAAGGGATGAGGATGA
PRIMER_RIGHT_2_SEQUENCE=TAATGCTCTTTCTTCCATGCAC
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.365
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.7439
PRIMER_LEFT_3_PENALTY=0.109273
PRIMER_RIGHT_3_PENALTY=0.634599
PRIMER_LEFT_3_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_3_SEQUENCE=TAATGCTCTTTCTTCCATGCAC
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=160,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.365
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.8678
PRIMER_LEFT_4_PENALTY=0.233224
PRIMER_RIGHT_4_PENALTY=0.634599
PRIMER_LEFT_4_SEQUENCE=GAGCTCTAGGGATGAGGATGTG
PRIMER_RIGHT_4_SEQUENCE=TAATGCTCTTTCTTCCATGCAC
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=160,22
PRIMER_LEFT_4_TM=60.233
PRIMER_RIGHT_4_TM=59.365
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_00474 Ps_contig_mira-and-tgicl-ass_franssen_4988
SEQUENCE=TCTTTCTTTCTCCAATGCCACTCTCATTCCCTTTTGTTTTGTGCTTCTCTATGACGATCATCATCCCTTAATTTCTTTCTTTCTTCATAATCATCCCCGTCCCTCAAGGTCTTTCTTCCTGTACGTTCATCCTCAGTCCCTCTAATCTTTGTTTCTATGTGACCACCCTCATCCCTCAAGTGCTTTCTTCCTATAGGAAAATCTTCATCCTTTCCATGTTTTCTCTCTCCACGACCATCTTCATCACCTCTTAAGCTCTTTTTCTCTTGGTGAACTTCCCTGCCTCTTTTATGTTTTCCATCTCTTGAATCATCCTCATCCCTTAAGTGCTTTCTTTCTATGCGCTCGTCTTCATTCCTTCCATGTTTTTTTTCTCCATGGTTATCCTGATCCTGTTTATAATCATTATCATGTCTTCCCTTCCTTCTTGTATCATAATCCTCCCTATACCGATCAATTCGTCCAACAGATGGTGATTCAACATCCTTGTTATAATTTCTGCCTCTCTGCTCAGAAGTCTCAATATCATTGCCTCTGAGCCTAGCTGACTTAGAATAACCCTCTTTTTCTGGATTTTCATTTTGATAAATTCGAGATTTTGACTCAGACTTCCTACCCTCTCCATCATGCTTAGCCAATTTGAAAGTCTCCCTATCTCTATCTTCATCAATCTTCTTGTCACCCTTTGCCATTGCTTGACTTCTAGAATTTGGATCCTCTTTAAAATCTCTCCCGTATGATTTTCTTAAAGTATCCAATCCATCAGTATTAGCACTACTTTTCSCBTTTTCGGCAGCAATACTCTGTACTATTTATTTCTCCTTCG
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00475 Ps_contig_mira-and-tgicl-ass_franssen_4989
SEQUENCE=AAAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAGCACAGAGGGGGTGAGGATTATCATGGAAGAAAGAAGCGCAAGGAAGGGATGATGATGATCATGTGCAGTGGAAGAAAGGAGCTATTAGAGGAAGGNTGATGATGATGGAAGAAAAGTAAACGTACGAAGGG
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1829
PRIMER_LEFT_PENALTY=0.109273
PRIMER_RIGHT_PENALTY=0.073614
PRIMER_LEFT_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_SEQUENCE=TCTTCCATCATCATCANCCTTC
PRIMER_LEFT=33,22
PRIMER_RIGHT=190,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.9965
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.922591
PRIMER_LEFT_1_SEQUENCE=AGAACTCAAGGGATGAGGATGA
PRIMER_RIGHT_1_SEQUENCE=TAGCTCCTTTCTTCCACTGCAC
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=162,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.923
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=1.0246
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.950696
PRIMER_LEFT_2_SEQUENCE=AGAACTCAAGGGATGAGGATGA
PRIMER_RIGHT_2_SEQUENCE=AATAGCTCCTTTCTTCCACTGC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.049
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=1.1558
PRIMER_LEFT_3_PENALTY=0.233224
PRIMER_RIGHT_3_PENALTY=0.922591
PRIMER_LEFT_3_SEQUENCE=GAGCTCTAGGGATGAGGATGTG
PRIMER_RIGHT_3_SEQUENCE=TAGCTCCTTTCTTCCACTGCAC
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=60.233
PRIMER_RIGHT_3_TM=60.923
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=1.1839
PRIMER_LEFT_4_PENALTY=0.233224
PRIMER_RIGHT_4_PENALTY=0.950696
PRIMER_LEFT_4_SEQUENCE=GAGCTCTAGGGATGAGGATGTG
PRIMER_RIGHT_4_SEQUENCE=AATAGCTCCTTTCTTCCACTGC
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=60.233
PRIMER_RIGHT_4_TM=59.049
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_00476 Ps_contig_mira-and-tgicl-ass_franssen_4993
SEQUENCE=TGTGTTTGAGTTGCAAGTAAAACTTCTCAAGCAAGTTCCAGAAGAGTATCCATATAAGGATGATGAGTAGTAATGTTCTGTTTTGTATTGTTGTACTTCATCATCCCAAGTATTAGATTATTATTATTATTATCTTTCTTGCCTTGTRTGCATTTTGACAAAACTAAAAATAACGATAGAAAATATTTAATTTAAATCATTATGATAATTGGGATTTAARACTTAAAAAA
TARGET=117,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00477 Ps_contig_mira-and-tgicl-ass_franssen_4994
SEQUENCE=AATTCTTAACTTATAAAYCCCTAATTATCATAATGTATTTAATTAAATTATTTTCTATCGTTATTTTTTAGTTTTGTACAAAATGCACACAAGGCAGAAAGATAATAATAATAATCTAATACTTGGGATGATGAAGTACAACAATAACAAAACAGAACATTACTACTCATCATCCTTATATGGATACTCTTCTGGAACTTGCTTGAGTAAGTTTTCTGCAACTACAAACAC
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00478 Ps_contig_mira-and-tgicl-ass_franssen_4997
SEQUENCE=CAACCCTCTGAACCATTGTAAATTGAAATATTCTCCTCTTCCTCAGGTCGACATCCACGAACCCAAATCCGTTGTTTATACTTGTTCTCCTCCACAAATAAACTACTCATGATCTTTCTTTCTTTGTGACCCAATAAAACTCTGTAAGTAGAATGATGAAAAATAACGCCTGAACCTTTCAAACTGCACAACAAGAGTTTAGCTATTTGCTTTACCATCAACATATACAATACACTATATAGTTGCAATCAAATTAAAAAGATGTGTTATTGCTTAATAAAAAAAGACGTTGTGTTATTTTTGGCACAAAATAGGAACAAA
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3084
PRIMER_LEFT_PENALTY=0.157667
PRIMER_RIGHT_PENALTY=0.150698
PRIMER_LEFT_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_SEQUENCE=GGTTCAGGCGTTATTTTTCATC
PRIMER_LEFT=28,22
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=59.842
PRIMER_RIGHT_TM=59.849
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.3199
PRIMER_LEFT_1_PENALTY=0.157667
PRIMER_RIGHT_1_PENALTY=0.162217
PRIMER_LEFT_1_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_1_SEQUENCE=AGTTTGAAAGGTTCAGGCGTTA
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=59.842
PRIMER_RIGHT_1_TM=60.162
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.3473
PRIMER_LEFT_2_PENALTY=0.157667
PRIMER_RIGHT_2_PENALTY=0.189596
PRIMER_LEFT_2_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_2_SEQUENCE=TGTTGTGCAGTTTGAAAGGTTC
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=59.842
PRIMER_RIGHT_2_TM=60.190
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.4755
PRIMER_LEFT_3_PENALTY=0.157667
PRIMER_RIGHT_3_PENALTY=0.317850
PRIMER_LEFT_3_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_3_SEQUENCE=TCTTGTTGTGCAGTTTGAAAGG
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=59.842
PRIMER_RIGHT_3_TM=60.318
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.4755
PRIMER_LEFT_4_PENALTY=0.157667
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_4_SEQUENCE=TTGTGCAGTTTGAAAGGTTCAG
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=190,22
PRIMER_LEFT_4_TM=59.842
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_00479 Ps_contig_mira-and-tgicl-ass_franssen_5015
SEQUENCE=CCATCTGGAGRWTTGTTTTTGTAGTACTAAGGGCTCGATGGAGCCTTCAGTCAGCCCTGGTAGCTCTGAGAGGCTGCCAGAGTAAAAATCTGACATTGGGCTTTTTTCTTTCAGTGCTGGTGACGGAGAAAAAGGTTCGCCAAATATTTGAGGTAATGCTGTTGGAGTTGGGGCATTTCCACCTTCCTCGATAGCTGAGATAGGTATAGAGTTCATGGTGCAGTGCACTCTTCTTGGCCCTCTAGTCCAGAGAGCATTCAGCTCATAGGAAACAGTGCTAACAAGATTGCTACATGCTGGAACTCTTGGTGACACCTGCTTAGAATTAAATATCCCACTTGGACGATGCTTTGGTTGAACTGCAGCATCATGCGGGGGTTGACTGTCGTATACAGTGAATTTGGTGCCCCAAAAATTAGACCTCAGTTTACCAACATATTTGTTGCTGGCTCGAGAAAAATTGTCTGCAGCCAAGGATATGATAAAGCCGGTACCTACGGCCCTCCTTATCTTTCTGGCTGCTAGTAACAACTTATTACTCTCATGCTCCGATGGCACCAAACCTAAGTACAACACGAACGTAGAAGTTTCTCTGTTCCTCCTAATAAAGCACTGTATTGGATAATCACGGGGACCGGGCTGCTTCAATGAAATAGGAAATGTGATCCTTCCACATTGCTGAGGAGTCTTGATGATCTCTTTCATAACAGACCTCCATGATTTACATACCGAAGCACAAAAGACAACAACAGCACGTGCAGGCCAAGATGTCTCACTCTCTTCGACCCTCCGGATTATATCCAAAAGCAATTCAGGTGGTAGACTTGCCCACTGTCCCTGTTGAATAGGTTCAGTCGGAGTTACATCCGGAGCAACATGTGACTTCGACCGAGAGAGCCAATGCTTGCTCTCACCACCCCGCCTTGATATGCTACCTATTCCATTTTTCATCCCCTTGAGTTCACGGACTATACTTTTCAGCGACATGATTCCACGCCTTACAAACTATGTCAATCCAACCACAAGAAGCTTCTAGATTTCTGATGCTAACTGAATGAAARAAAAAAAGAGATAAATAGCAACAAGTTTAGAATACCGATCTACTAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAACGTTATTTGAATTTGTGGCGAAAATTAGTTGACATCGCTCAGTTAAGCGCCACCGCGATTCTGCGGAATCGCGTTTGTGGAATTAGATCAAGCTTTTTTTATCATGTCTCATTTTTATAATAATTGATTATTTCSSTC
TARGET=1106,45
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00480 Ps_contig_mira-and-tgicl-ass_franssen_5018
SEQUENCE=TCGTAATATGACATTCTCAACATGTAATCAACAAGAATTCGCTTCAAGCGAGTGTTATTCCATTCCGACATTTTTCTGCATCTGCAGATTCCAAATGTTCGAGACGTGCACGACACTTCTGCGCTTGCAGATGCTCAGCTCGATTTCCTTCTTCCAATTTTCTTTTGAGTCCTTGGAGGCGGGAGACGAGAGAGCTAAGGTGATTAACGGCGTCGGAGGGGGATAAATCAGAAGAGACGGCATCGTTGACGCCGGAGATAACGGCGGTCATCTCCTTCTCGACGACACGGTTGTTGGCTCGGAGGGTTTTCTTGAAGTGCTCGAAAGGGACTCTGAGGAACTGATGTTCGAGCTTGAGAGACTCCGTCAGTCGGGGAAGGTTGGAGGACGGAGCAGGCGTAGGGTTGACGGCGGCGGTGGCTCCGATTTGAGTTCCGGTGGCAGTGTTGTTTCCGTTCGGAGTGGAATCCATTTCCATTGGGGAAGGAAGGAACGAAAAATTGGAATCTCACAGATTGCAACTTGTAACTGGATAAATGCTCTTACAAAACTAAATCC
TARGET=482,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4339
PRIMER_LEFT_PENALTY=0.023267
PRIMER_RIGHT_PENALTY=3.410595
PRIMER_LEFT_SEQUENCE=ATGTTCGAGCTTGAGAGACTCC
PRIMER_RIGHT_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT=343,22
PRIMER_RIGHT=541,25
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=60.411
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=3.5546
PRIMER_LEFT_1_PENALTY=0.143960
PRIMER_RIGHT_1_PENALTY=3.410595
PRIMER_LEFT_1_SEQUENCE=GAAAGGGACTCTGAGGAACTGA
PRIMER_RIGHT_1_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_1=322,22
PRIMER_RIGHT_1=541,25
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=60.411
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=3.6101
PRIMER_LEFT_2_PENALTY=0.199477
PRIMER_RIGHT_2_PENALTY=3.410595
PRIMER_LEFT_2_SEQUENCE=GAGCTTGAGAGACTCCGTCAGT
PRIMER_RIGHT_2_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_2=349,22
PRIMER_RIGHT_2=541,25
PRIMER_LEFT_2_TM=60.199
PRIMER_RIGHT_2_TM=60.411
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=3.6713
PRIMER_LEFT_3_PENALTY=0.260719
PRIMER_RIGHT_3_PENALTY=3.410595
PRIMER_LEFT_3_SEQUENCE=AAGGGACTCTGAGGAACTGATG
PRIMER_RIGHT_3_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_3=324,22
PRIMER_RIGHT_3=541,25
PRIMER_LEFT_3_TM=59.739
PRIMER_RIGHT_3_TM=60.411
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=3.8061
PRIMER_LEFT_4_PENALTY=0.395499
PRIMER_RIGHT_4_PENALTY=3.410595
PRIMER_LEFT_4_SEQUENCE=GGGACTCTGAGGAACTGATGTT
PRIMER_RIGHT_4_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_4=326,22
PRIMER_RIGHT_4=541,25
PRIMER_LEFT_4_TM=59.605
PRIMER_RIGHT_4_TM=60.411
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_00481 Ps_contig_mira-and-tgicl-ass_franssen_5023
SEQUENCE=GAGAGCAGTGGTTTTTACGACGGCGAAATTGAGGTCGATGTTGTTGTTGTTGAGGATAGGGAAGACGGTGATGATGATGAAGGAAATCAGTAGATAATGATGAATTGAGTGGTTGTGGAAGAAAGCGGAGAAACGCCATGTTCACGGTGGTGATGCTGCAGATCTATACTCGGAGAAGATGATTGTTTGTTGTATTYGTTACTACCTCCCCGCGTACTCTGCGTT
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00482 Ps_contig_mira-and-tgicl-ass_franssen_5023
SEQUENCE=GAGAGCAGTGGTTTTTACGACGGCGAAATTGAGGTCGATGTTGTTGTTGTTGAGGATAGGGAAGACGGTGATGATGATGAAGGAAATCAGTAGATAATGATGAATTGAGTGGTTGTGGAAGAAAGCGGAGAAACGCCATGTTCACGGTGGTGATGCTGCAGATCTATACTCGGAGAAGATGATTGTTTGTTGTATTYGTTACTACCTCCCCGCGTACTCTGCGTT
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00483 Ps_contig_mira-and-tgicl-ass_franssen_5026
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTTAAAAATTTTAACAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGTGGTGGGAACATGAGAATTTGTTATT
TARGET=5,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00484 Ps_contig_mira-and-tgicl-ass_franssen_5026
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTTAAAAATTTTAACAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGTGGTGGGAACATGAGAATTTGTTATT
TARGET=44,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00485 Ps_contig_mira-and-tgicl-ass_franssen_5027
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTAAAAATTTAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGCGGTTGGGAAACATGAGAATTTGTATCAAATTCAGAAGAAGGATGTGTTGGAATTATTGAAGTTACTATGAAAAGACAAATTAATCTGTAC
TARGET=5,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00486 Ps_contig_mira-and-tgicl-ass_franssen_5027
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTAAAAATTTAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGCGGTTGGGAAACATGAGAATTTGTATCAAATTCAGAAGAAGGATGTGTTGGAATTATTGAAGTTACTATGAAAAGACAAATTAATCTGTAC
TARGET=39,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00487 Ps_contig_mira-and-tgicl-ass_franssen_5028
SEQUENCE=TCGGGGATACTGCCATGGCCACACGTTCATAAGAAAAGAAAAAGNGAGAGTGAAACTTGTAATAGAGTAAGGAAAGCTTTTGCGAAAGAGTCAGAATAATCAACATTCATCATCATCATCATCATCATCTAGAATGGCCACTATATCATCATCTTTGCCAACAAATAGTAAACCTNTTTTCTCAGTATCNGCAAGACCACTACTTTCTTTGTTCCTTT
TARGET=107,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2208
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.193133
PRIMER_LEFT_SEQUENCE=GCTTTTGCGAAAGAGTCAGAAT
PRIMER_RIGHT_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT=75,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.807
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.3752
PRIMER_LEFT_1_PENALTY=0.182061
PRIMER_RIGHT_1_PENALTY=0.193133
PRIMER_LEFT_1_SEQUENCE=AAAGCTTTTGCGAAAGAGTCAG
PRIMER_RIGHT_1_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=198,22
PRIMER_LEFT_1_TM=60.182
PRIMER_RIGHT_1_TM=59.807
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=0.5057
PRIMER_LEFT_2_PENALTY=0.312541
PRIMER_RIGHT_2_PENALTY=0.193133
PRIMER_LEFT_2_SEQUENCE=AGAGTAAGGAAAGCTTTTGCGA
PRIMER_RIGHT_2_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=59.687
PRIMER_RIGHT_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.7310
PRIMER_LEFT_3_PENALTY=0.027715
PRIMER_RIGHT_3_PENALTY=0.703266
PRIMER_LEFT_3_SEQUENCE=GCTTTTGCGAAAGAGTCAGAAT
PRIMER_RIGHT_3_SEQUENCE=GTGGTCTTGCNGATACTGAGAA
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=59.297
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.8543
PRIMER_LEFT_4_PENALTY=0.661199
PRIMER_RIGHT_4_PENALTY=0.193133
PRIMER_LEFT_4_SEQUENCE=AGCTTTTGCGAAAGAGTCAGAA
PRIMER_RIGHT_4_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=198,22
PRIMER_LEFT_4_TM=60.661
PRIMER_RIGHT_4_TM=59.807
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_00488 Ps_contig_mira-and-tgicl-ass_franssen_5033
SEQUENCE=TACCACGAAACGGAAGTACAACGAAACCGAAAAAAGGAAAAAGGAAATAGAAGAAAGAAAGAAACAAGTGAAACTAGATCAGTTCCGAGGGTTTCGGTAAWKTWCGTTGGTTTGGTACAAAGAAAGACTGCCATGGTTTCTTCAGACCATTCGCAACATGCTTACTTCTCCAGGGAGTTTCGCTGAAAGCCAACACGGAGAAGTGACTTTTCCTGAGATCAGTACCACTATTCTTGAGAAGATTTGCCAGTATTTTTATTGGCATCTTGAATTCGCCAGTGGAAAGGACAGAGAGTTTCCTATTGAACCGGAATTAACTCTGGAGTTAATGATGGCTGCCAATTACCTACATACTTGAGCATATTCACTTCCCAAAGGTAATGCAGAAGAAACTGTTGCTTTGCTGGGCTATGTAAAAATACAAGCTACTATTGTTGTTGTCGTTATAAACTAGAATATACATGTAAGGGGAGATGTCAACTCCTCGTCTTACTTCTTTGTAGTCTTTTAATTTTGGGCTTTGACACTTAATATTTGTTAAAAATAATATGATAGCTAGCTAGATTCAGCTGTTAGACTGACCTGATTGATAATATGATTAGTTAGATTCAGACTWAAGTT
TARGET=51,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00489 Ps_contig_mira-and-tgicl-ass_franssen_5037
SEQUENCE=ACGTCGGGGATGGGTAGAAAAGAAGAAGAAGAAGTGGCGGTGGGAGTACCAGTTTATGAAAGAAACGGCATCCCACCAAACGCGTTGATCGGAGATCCCAAAGGTATTCCCATCCAACAGACTATTTACAGAGACACTCCTGCTCCCTTCAATTGTCCTCACTGCGGTCACACCTCTCTCACCTCTATCCAAGATTAGTCTGGCTGCATTCGTTAGGATGCTTGATGCCGA
TARGET=20,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00490 Ps_contig_mira-and-tgicl-ass_franssen_5052
SEQUENCE=GCAGTGGTTATTCAACGCAGAGTTATTTATATTAAAGTTTTTTTTTTAATTTAAAATCTTTCCATTTTTCCTACGGCGGCGGCGGCTACCAAACAGGACACGACATAACACAGCCCTCCATCAAGTGAATCGAAGGAAATTTCTGACCATGGCTTCAACCATTCCTGTTGAACAAGTCAACAGTGGTCCGCTTCAGGTTACTGGTGACTCCTTTATCAGACAACATCTTAGGAAATTGGCTCCTTATCAACCCATTTTGCCTTTTGAGGTTTTATCTTCTCGTCTTGGACGGAAGCCGGAGGATATTGTCAAGTTAGATGCTAATGAGAATCCTTACGGCCCTCCTCCAGAGGTAATGGAAGCCCTAGGGTCAATACGATTCCCATATGTCTATCCAGATCCAGAGAGCCGTCGGTTGCGTGAAGCTCTCGCTCAAGATTCAGGCCTTGAATCTGAGTATATTCTTGTAGGATGTGGTGCCGACGAGCTTATTGATTTGATCATGCGTTGTGTGCTTGATCCTGGTGACAAGATCGTCGACTGCCCTCCAACTTTCACAATGTATGAATTTGATGCCGCAGTTAACGGAGCACTCGTCATCAAAGTTCCAAGGAAGCCAGACTTCAGCTTGAATGTCGAACACATAATCGAGGTTGTTGAACAAGAGAAGCCCAAATGCATATTTTTAACATCTCCTAACAATCCAGATGGGAGTGTAATTAATGACGATGATCTCTTAAAGATACTCGAGCTTCCCATACTGGTGGTGCTGGATGAAGCATACATCGAGTTTTCACAAATTGAATCAAAGATGAGTTGGGTGAAGAAACATGAGAATCTCATTATTCTTCGAACATTCAGCAAAAGAGCTGGTTTAGCTGGACTTCGTGTGGGATATGGAGCTTTTCCTTTGAGTTTAATTAAGTATCTTTGGAGAGCAAAGCAACCTTATAATGTATCCGTTGCAGCCGAAGTTTCTGCATGCGCAGCACTGGAAAATCCTACCTATCTAGAGAATGTGAAAGATGCTTTGGTGAAAGAAAGAGGGAGGCTTTTTGAGCTTTTGAAGGCAGTTCCATTCCTTAGGCCATTTCCAAGCCATTCTAATTTCATTCTTTGTGAGGTTACATCTGGATTTGATGCAAAGAAGTTAAAGGATGATCTTGCAGCAATGGGCGTGATGATTCGTCATTACAGCAGTAAAGAGCTGAAAGGCTACGTTCGAGTATCGGTTGGGAAGCCTGAACACACGGACGCGCTTATGAACGGCCTCAACAGACTATTGTAGGAGAAAAATTTCACTGTGATCGATCGATCATACAAAGAGATTCTCTTACATAACATTCAAAACTAGAGGAAAATGTATTATGAGAAGTTGAGCTAGTTTATGTTATGCAGACAATAATGAGTTCATCTCAGATATACAACACCAACGAACGTAATCTTAAGTTGTAACATTTGTATTATGTATTTTAAGGTGGTTGATTCAGGACATAAGTTACTTCCTTGTTTAATAAATTATCTTTAAGGTGAGAAATCAAAATC
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6993
PRIMER_LEFT_PENALTY=0.691033
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_SEQUENCE=TTGACTTGTTCAACAGGAATGG
PRIMER_LEFT=1,22
PRIMER_RIGHT=179,22
PRIMER_LEFT_TM=59.309
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.7944
PRIMER_LEFT_1_PENALTY=0.691033
PRIMER_RIGHT_1_PENALTY=0.103353
PRIMER_LEFT_1_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_1_SEQUENCE=GCTGTGTTATGTCGTGTCCTGT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=113,22
PRIMER_LEFT_1_TM=59.309
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.7992
PRIMER_LEFT_2_PENALTY=0.691033
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_2_SEQUENCE=ACTTGATGGAGGGCTGTGTTAT
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=125,22
PRIMER_LEFT_2_TM=59.309
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.8656
PRIMER_LEFT_3_PENALTY=0.691033
PRIMER_RIGHT_3_PENALTY=0.174518
PRIMER_LEFT_3_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_3_SEQUENCE=GGAGTCACCAGTAACCTGAAGC
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=59.309
PRIMER_RIGHT_3_TM=60.175
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=1.1947
PRIMER_LEFT_4_PENALTY=0.691033
PRIMER_RIGHT_4_PENALTY=0.503696
PRIMER_LEFT_4_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_4_SEQUENCE=TGACTTGTTCAACAGGAATGGT
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=59.309
PRIMER_RIGHT_4_TM=59.496
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_00491 Ps_contig_mira-and-tgicl-ass_franssen_5052
SEQUENCE=GCAGTGGTTATTCAACGCAGAGTTATTTATATTAAAGTTTTTTTTTTAATTTAAAATCTTTCCATTTTTCCTACGGCGGCGGCGGCTACCAAACAGGACACGACATAACACAGCCCTCCATCAAGTGAATCGAAGGAAATTTCTGACCATGGCTTCAACCATTCCTGTTGAACAAGTCAACAGTGGTCCGCTTCAGGTTACTGGTGACTCCTTTATCAGACAACATCTTAGGAAATTGGCTCCTTATCAACCCATTTTGCCTTTTGAGGTTTTATCTTCTCGTCTTGGACGGAAGCCGGAGGATATTGTCAAGTTAGATGCTAATGAGAATCCTTACGGCCCTCCTCCAGAGGTAATGGAAGCCCTAGGGTCAATACGATTCCCATATGTCTATCCAGATCCAGAGAGCCGTCGGTTGCGTGAAGCTCTCGCTCAAGATTCAGGCCTTGAATCTGAGTATATTCTTGTAGGATGTGGTGCCGACGAGCTTATTGATTTGATCATGCGTTGTGTGCTTGATCCTGGTGACAAGATCGTCGACTGCCCTCCAACTTTCACAATGTATGAATTTGATGCCGCAGTTAACGGAGCACTCGTCATCAAAGTTCCAAGGAAGCCAGACTTCAGCTTGAATGTCGAACACATAATCGAGGTTGTTGAACAAGAGAAGCCCAAATGCATATTTTTAACATCTCCTAACAATCCAGATGGGAGTGTAATTAATGACGATGATCTCTTAAAGATACTCGAGCTTCCCATACTGGTGGTGCTGGATGAAGCATACATCGAGTTTTCACAAATTGAATCAAAGATGAGTTGGGTGAAGAAACATGAGAATCTCATTATTCTTCGAACATTCAGCAAAAGAGCTGGTTTAGCTGGACTTCGTGTGGGATATGGAGCTTTTCCTTTGAGTTTAATTAAGTATCTTTGGAGAGCAAAGCAACCTTATAATGTATCCGTTGCAGCCGAAGTTTCTGCATGCGCAGCACTGGAAAATCCTACCTATCTAGAGAATGTGAAAGATGCTTTGGTGAAAGAAAGAGGGAGGCTTTTTGAGCTTTTGAAGGCAGTTCCATTCCTTAGGCCATTTCCAAGCCATTCTAATTTCATTCTTTGTGAGGTTACATCTGGATTTGATGCAAAGAAGTTAAAGGATGATCTTGCAGCAATGGGCGTGATGATTCGTCATTACAGCAGTAAAGAGCTGAAAGGCTACGTTCGAGTATCGGTTGGGAAGCCTGAACACACGGACGCGCTTATGAACGGCCTCAACAGACTATTGTAGGAGAAAAATTTCACTGTGATCGATCGATCATACAAAGAGATTCTCTTACATAACATTCAAAACTAGAGGAAAATGTATTATGAGAAGTTGAGCTAGTTTATGTTATGCAGACAATAATGAGTTCATCTCAGATATACAACACCAACGAACGTAATCTTAAGTTGTAACATTTGTATTATGTATTTTAAGGTGGTTGATTCAGGACATAAGTTACTTCCTTGTTTAATAAATTATCTTTAAGGTGAGAAATCAAAATC
TARGET=1306,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9613
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.935692
PRIMER_LEFT_SEQUENCE=ATGAACGGCCTCAACAGACTAT
PRIMER_RIGHT_SEQUENCE=AAGATTACGTTCGTTGGTGTTG
PRIMER_LEFT=1261,22
PRIMER_RIGHT=1445,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=59.064
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=1.1670
PRIMER_LEFT_1_PENALTY=0.231323
PRIMER_RIGHT_1_PENALTY=0.935692
PRIMER_LEFT_1_SEQUENCE=TATGAACGGCCTCAACAGACTA
PRIMER_RIGHT_1_SEQUENCE=AAGATTACGTTCGTTGGTGTTG
PRIMER_LEFT_1=1260,22
PRIMER_RIGHT_1=1445,22
PRIMER_LEFT_1_TM=59.769
PRIMER_RIGHT_1_TM=59.064
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=1.4642
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=1.438617
PRIMER_LEFT_2_SEQUENCE=ATGAACGGCCTCAACAGACTAT
PRIMER_RIGHT_2_SEQUENCE=AGATTACGTTCGTTGGTGTTGT
PRIMER_LEFT_2=1261,22
PRIMER_RIGHT_2=1444,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=58.561
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=1.4672
PRIMER_LEFT_3_PENALTY=0.531474
PRIMER_RIGHT_3_PENALTY=0.935692
PRIMER_LEFT_3_SEQUENCE=CGTGATGATTCGTCATTACAGC
PRIMER_RIGHT_3_SEQUENCE=AAGATTACGTTCGTTGGTGTTG
PRIMER_LEFT_3=1176,22
PRIMER_RIGHT_3=1445,22
PRIMER_LEFT_3_TM=60.531
PRIMER_RIGHT_3_TM=59.064
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=1.5893
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=1.563720
PRIMER_LEFT_4_SEQUENCE=ATGAACGGCCTCAACAGACTAT
PRIMER_RIGHT_4_SEQUENCE=TATGTCCTGAATCAACCACCTT
PRIMER_LEFT_4=1261,22
PRIMER_RIGHT_4=1495,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=58.436
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_00492 Ps_contig_mira-and-tgicl-ass_franssen_5061
SEQUENCE=ACGTTAAAAAATTACATCAATTTTCCATTACTATGATCAACCAATGTAAACGGGATAGTACTGCAACCAGTTGTTAGTAATACAAAAACAGAATATAGAATTCCATCATTTCATCTTTTGTGTGTGTCTTGCTAATCTTTGGATTCAAAATCTTGTTACAAAGGGTGATCAGACATCAGCAATGATACCAGCTAACAAGAAGAATGGGAAGGTGCGTAAAATCGTTTCCCAACAAACTATTGTACAAAATGAATACTACTTTGAAGCTCTGGRAAAAAAGGTCGCTGCCTTCATCATTGAGATGCTAGAGTAGTACTCGTAGTCTCCGGCTTGGTTTCAACAGGGCACCCTCCCTTAACAACTGTGGCGGCCATTCTATTTGGTGGCCAATAGCGGATTATCGATCTACCTATGATATTCTTGGCAGGCAGGGGACCCCACACATGTGAGTCGTCGCTGTTATTGCGGTTGTCTCCCATCACAAAAACGGAGTTTTCTGGCACACGTATGGGTTTCATCTCATACTTCGGTGCCTCAATTATAAACTTCTCATCTCTCTCAACCCCATTAACTACAACATGTCCATTTCGAACCTCCACGATATCACCTTCCTTTGCAACAACGCGTTTTATTAAAACATCATCATCAGTATATTTAACTTCTTGAAGATCTGGAGGGCTTTTAAAAACCACAATATCATTTGCACACGGCTTTCTAAAATAGTATGAAACCTTTTCCGCAACAATTCCATCTCCAATATCATACGTAGGATACATTGACATAGAAGGAATAAACCTCGGTTCAGCAATGAAAGTACGAAAAACAAGTGAAATTGCCAAAGCAGCCAAAACCGTTTTGGCATCATCAGTAGTGAAATTCAACCACTCTGGAAAAAGTTCGGACGACACATCATTCATCTCAACCTCCTGGTCACCATCTCCACCATCACCACCACCACCTCCACCGCCACCATCACCGGAATCTTTCAAAGCTTTATGGCATGGGAATCTACGGCGAAGAGTGGTGGAGGTTTTGTAGAGTTGGGGAAAGTTGGGACGAGAGCGTGGCGGTGAAGAGATTCTAGAACTGAAACGGAGATTGGATAATTTGAGGGAATTGAAGAGATAGGGATGGTCCAAGTTAGGGTTTTGAAGTGATGGTAGTGAAGAAGAGATAGAGAATGAAGATGACATAGTTGAGTTGAATTGAAAACGGTTAAATGAGTTTYC
TARGET=947,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00493 Ps_contig_mira-and-tgicl-ass_franssen_5069
SEQUENCE=GGGATTCTGAGTGCTCTCTCCAATCTCAAATCTCATTCCCTTCACAAAATCTCTGAAGCCACAAACTTGTTCTTTCGCACTTGTATTAATTGCTTCCAAACTCTCTCTCTCTCTCTCTTCTCTCTCTGCCCCAAATATGGAGGCCATCACTGCTTCCTTAGAGAGGTCTCTTCAAAACTGTTCCCTAAACAACCACCACCACCACCACCACCAACAGAACGGAAGGAGACAAGACGACGGTTCCGCCACCACAGATGCACCGGCCGCAGAAGGAGAAGGAGGAGGCATAGGAATCTCATCCACCAG
TARGET=101,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1056
PRIMER_LEFT_PENALTY=0.043988
PRIMER_RIGHT_PENALTY=0.061624
PRIMER_LEFT_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_SEQUENCE=GATTCCTATGCCTCCTCCTTCT
PRIMER_LEFT=61,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.956
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.1056
PRIMER_LEFT_1_PENALTY=0.043988
PRIMER_RIGHT_1_PENALTY=0.061624
PRIMER_LEFT_1_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_1_SEQUENCE=GAGATTCCTATGCCTCCTCCTT
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=59.956
PRIMER_RIGHT_1_TM=60.062
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.1056
PRIMER_LEFT_2_PENALTY=0.043988
PRIMER_RIGHT_2_PENALTY=0.061624
PRIMER_LEFT_2_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_2_SEQUENCE=ATTCCTATGCCTCCTCCTTCTC
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.956
PRIMER_RIGHT_2_TM=60.062
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1056
PRIMER_LEFT_3_PENALTY=0.043988
PRIMER_RIGHT_3_PENALTY=0.061624
PRIMER_LEFT_3_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_3_SEQUENCE=AGATTCCTATGCCTCCTCCTTC
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.1125
PRIMER_LEFT_4_PENALTY=0.043988
PRIMER_RIGHT_4_PENALTY=0.068521
PRIMER_LEFT_4_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_4_SEQUENCE=GTGGATGAGATTCCTATGCCTC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=59.956
PRIMER_RIGHT_4_TM=59.931
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_00494 Ps_contig_mira-and-tgicl-ass_franssen_5069
SEQUENCE=GGGATTCTGAGTGCTCTCTCCAATCTCAAATCTCATTCCCTTCACAAAATCTCTGAAGCCACAAACTTGTTCTTTCGCACTTGTATTAATTGCTTCCAAACTCTCTCTCTCTCTCTCTTCTCTCTCTGCCCCAAATATGGAGGCCATCACTGCTTCCTTAGAGAGGTCTCTTCAAAACTGTTCCCTAAACAACCACCACCACCACCACCACCAACAGAACGGAAGGAGACAAGACGACGGTTCCGCCACCACAGATGCACCGGCCGCAGAAGGAGAAGGAGGAGGCATAGGAATCTCATCCACCAG
TARGET=192,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1056
PRIMER_LEFT_PENALTY=0.043988
PRIMER_RIGHT_PENALTY=0.061624
PRIMER_LEFT_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_SEQUENCE=GATTCCTATGCCTCCTCCTTCT
PRIMER_LEFT=61,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.956
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.1056
PRIMER_LEFT_1_PENALTY=0.043988
PRIMER_RIGHT_1_PENALTY=0.061624
PRIMER_LEFT_1_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_1_SEQUENCE=GAGATTCCTATGCCTCCTCCTT
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=59.956
PRIMER_RIGHT_1_TM=60.062
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.1056
PRIMER_LEFT_2_PENALTY=0.043988
PRIMER_RIGHT_2_PENALTY=0.061624
PRIMER_LEFT_2_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_2_SEQUENCE=ATTCCTATGCCTCCTCCTTCTC
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.956
PRIMER_RIGHT_2_TM=60.062
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1056
PRIMER_LEFT_3_PENALTY=0.043988
PRIMER_RIGHT_3_PENALTY=0.061624
PRIMER_LEFT_3_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_3_SEQUENCE=AGATTCCTATGCCTCCTCCTTC
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.1125
PRIMER_LEFT_4_PENALTY=0.043988
PRIMER_RIGHT_4_PENALTY=0.068521
PRIMER_LEFT_4_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_4_SEQUENCE=GTGGATGAGATTCCTATGCCTC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=59.956
PRIMER_RIGHT_4_TM=59.931
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_00495 Ps_contig_mira-and-tgicl-ass_franssen_5092
SEQUENCE=ACCATGGCATCGGTTTCGCCACCGGCTCGAGGATGTGGTGTTCCGATGGAATTCGGCAACACACAAGCTGCTGTTAGGATTGAAGGTTTTGACTGTCTTAGAAGAGATAGAAGGAGTATCTCTGCTGTAGCCTAATCCATTTTTGAAGAAAGAAAGAAGAACATAGAAAATAGTGAGAGAGAACACAGCTCAATAACCAAGAATCAGCTAGTTTAGTTTTTTTTTTCCTTAGGAAAGAGGATCTAATCTTAATAATTAGTAGTTGTTTGATGTATATATATGTGATGTTTGAAGTTTGATTAGTTAATACAAATAAAAAGAGAAGAAACAAATCAAGAAGTAAAACAAAGGTAAAGAAAAGAAGCCTATTTTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2900
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=1.270039
PRIMER_LEFT_SEQUENCE=AACACACAAGCTGCTGTTAGGA
PRIMER_RIGHT_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT=57,22
PRIMER_RIGHT=207,23
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.270
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=1.3571
PRIMER_LEFT_1_PENALTY=0.087031
PRIMER_RIGHT_1_PENALTY=1.270039
PRIMER_LEFT_1_SEQUENCE=AAGCTGCTGTTAGGATTGAAGG
PRIMER_RIGHT_1_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=207,23
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.270
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=1.4809
PRIMER_LEFT_2_PENALTY=0.210886
PRIMER_RIGHT_2_PENALTY=1.270039
PRIMER_LEFT_2_SEQUENCE=GCTGCTGTTAGGATTGAAGGTT
PRIMER_RIGHT_2_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=207,23
PRIMER_LEFT_2_TM=59.789
PRIMER_RIGHT_2_TM=60.270
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=1.7105
PRIMER_LEFT_3_PENALTY=0.440473
PRIMER_RIGHT_3_PENALTY=1.270039
PRIMER_LEFT_3_SEQUENCE=CACAAGCTGCTGTTAGGATTGA
PRIMER_RIGHT_3_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=207,23
PRIMER_LEFT_3_TM=60.440
PRIMER_RIGHT_3_TM=60.270
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=1.8226
PRIMER_LEFT_4_PENALTY=0.552581
PRIMER_RIGHT_4_PENALTY=1.270039
PRIMER_LEFT_4_SEQUENCE=ACACAAGCTGCTGTTAGGATTG
PRIMER_RIGHT_4_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=207,23
PRIMER_LEFT_4_TM=59.447
PRIMER_RIGHT_4_TM=60.270
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_00496 Ps_contig_mira-and-tgicl-ass_franssen_5093
SEQUENCE=GTACGTACCTACTTACNGTGGCGTCGTCNCNNCGGCTCGAGGATGTGGTGTTCCGATGGAATTCGGCAACACACAAGCTGCTGTTAGGATTGAAGGTTTTGACTGTCTTAGAAGAGATAGAAGGAGTATCTCTGCTGTAGCCTAATCCATTTTTGAAGAAAGAAAGAAGAACATAGAAAATAGTGAGAGAGAACACAGCTCAATACCAAGAATCAGCAGTAGTTTTTTTTCCTTTGKAARRAGGATCTAATCTTAATAATTAGTAGTGTTGTNTGTATATATAATTRTGNTGTTGTAAGTTGT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00497 Ps_contig_mira-and-tgicl-ass_franssen_5094
SEQUENCE=CCTTTTGGTAAAGTAGGATCTAATCTTAATAATTAGTAGTGTTTGATAGTAATAATAATAATTGTGATGTTTGAAGTTTGATGTTAATCAATAAAAGAGAGAACAAATCAGAGTAAACAAGGTAAGAAAAGAAGCCATTTTCTTCTGTTTKKTTATTAGGTTTGTGGAGAGTTAAGGTATCCTTCCTNTGTAATTGTTAATTTGGAGGAAATTGGTAATTTTGTTACATCCTATTATTGTTATTAATCATAATGTAGTTTTAAAAAGT
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00498 Ps_contig_mira-and-tgicl-ass_franssen_5095
SEQUENCE=CGTTTTTTTAAATTTACTTGTATAAATATAAAGTTAGATTTGTTATCCATTATTACTTTACTATGCACACGTATTATAATAAAAATAAAAATAAAATAAATAATATAAAGGGTTTGGTTATCTTTATGTAAACGACCTATGTGAATGGTGGGGCAATCAAAARSTKWCTCCACAAAGACTTGCTTTTTAATTTCTTAATAATAAAATATACACCCCCAATTATATTAAAATAATAATTAAACARATAATGCATTATTGGTTCTTCAATTTGTTTTTTTATGGACCCAAACCGGAAAAGAAGGAGCTTTCTTATATACCCTTTCCTTTTCTTTTCCATTTAATAATCATTCTTTCTCCAAAGGCTCCTATTCTTTTTTTCAACTTTATTGACAAAAAGAATTATTATAGAATAGTAACAACTATGTTTTTTTCCCACCAAATAGGGTTTGTATTGTATTGAAATTTGAATATGTACCAATTTGAATCCATTAGAATGAAGAACCAAAAGGTGCCCTATGAGGAATACCTTTTCTTCTTCTTGCATTTAAAGGAGGAGGAGGAGGTGTGATAGGATACATATCTTGTGTTGAACCTTCACGCAATTCTTCTTCTGATCTACTACTTGACACCCCCTCCATTCTCATTGCTTCATTCTTACCCCCTTCAATAGCAAAACTTGCATCTCCATTTTTCTTTATTCTTTCAAGCTTACATCCCAAAGAACAGAACCTAAATGAATCCAAGAGACTCCTTCCACAAATCTCACAATGTGAGCTACTCCTTTACAGATTTTGGTTGAGGCCTCACATTCAAGAACAATA
TARGET=549,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00499 Ps_contig_mira-and-tgicl-ass_franssen_5110
SEQUENCE=CAGAGAGAATCATTTGAAGATGTTTAAATTAGAAGATGTTGCAATGGGAATCTGGATCGCAGAAATGAAGAAGGAAGGTCTAAAAGTTCGATACGAAAATGAGGGCAGAGTTTATAACGAGGGTTGCAAGGATGGTTATGTGGTTGCCCATTATCAAGGTCCAAGGGAGATGCTATGTTTGTGGCACAAACTTGAGGAATTAAAACGTGCAACATGCTGTGGTGATAGGAGGTGACTACTGAATGCTGACTAACCAAGATCCCACTTCTGAATAAAAGWAACTTTTCCCCWTACTTTTTTTTAGTAATAAATAAATAGTAGAATAGTTAGCTAGCCAGATATGATTAGGAATAGGTTAA
TARGET=306,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00500 Ps_contig_mira-and-tgicl-ass_franssen_5113
SEQUENCE=TTTTTTTTAGTAACAGAAGCAAGATTATCCATTACTGAATAAAATAGCATTCATTTTACAACATTTGATAGGGATTCTTCCTCACACACACAAAAATAACAGCAACTAGCAACTAATAATCAAGGTTCTGCAATTCTTGCAAGGCTCTACAGTTTTTGATATTCGCGGTTATATGCAGCGCGATCAGAGACAGACATCAAAACCCTTGATGTTATATGTGACTGATCATCAGAGACATCAAAACCCTTGATGTTATTATATGCGACGATCATAGACATCAATCGCAGCAAACCTATTTTGAAACCATACTAAGACTAATAACTCTTCGGGCTTAAAATTTCCCTAATACTAGCAAGGCATGGATCCAAAAAACCATAAATCCAAATGCCTTGGAGGAATACACACTATGTGACTTGATCTTCAAAGATATATCTTCATTCTCACAAGTATATATACTGCTTCATCATATTACACCAGACCTACACTGTACAAGGATGAAGTCAGAAGATGCGGTTGCATACAAACACTGCCGCATCATCTCCCAGCACCGGTTAGGGTTCTCTCCAACACTTCAGAATTCGATGTCAATGAAGGTAGAGGAATCGACTCCGAACTCGCAGGTGCAGCTTCGGTCCCCAACTCTTCATTTACAGGCAGTGGTTGAGGCGAGTATGTAAAGGAAAGATCTTTGTTTTCGGATAGAGCTATGAGCTCTTTCTCCTCTAACCCTTTGGTTGGCCCCAAGCTGCACCTTTCTGGAGTGTGTTTGGCCAATGCATCTCTAAACTTCTTGATCGTGGCATTGGTGCAGCTAAAGCTGCATACTCGACCCTGAGCGCCTCTATAGAACCGGAAAAACGGAAGGACATGAACATTAAGACTGTAACACATAGATTTATGCTCCTCATAGTTCACAGAAAGGAACTCAACATCAGGATTCATCTCTGCAAATTGACATATCTTAGGATGAAGGGCTTTGCAGCCACCACAACCAGGAGAGAAAAAATCAACAATCACAAGTTTGTCCCCTGCGTTTAAAAGTGAATCTACAAGATCTTGTGCTGAAGTCACCTCCCTCATGTTAGGTTGAAGACCCTTTTCCCACCATTTTTGAGCTTTCCCTATTCTAAGTGTCATCTTAGGAGCAGCTGAAACTGAAAACTGTGAATTGATCCTTTTGGATGGTATTGATCTAGTTACACGAAAGAGAGGCTTTTTGCCATGAAACTCAGATGAAAAAGAAGATCTGAGAAACTGAGATTTCAGCTTCAAAGATGGAAAACTTCCGACACCAGTCTTGGGAACCATGGAAGCCCTTTGAAGCTGTTGATTTTTGTTTCCATGCCAAGAAGAAACCAAGCTTGTCTTGATCAAAGTTTCAGCCATTAAATATAAATTTTTCTTCACAAAAAAAAAGCTATTCGGTTACGTTTTGTTTCAGATTTTCTCGAGAATAGAAATTGATAAGATGAGAGAAAGAAAATGAAGACTTTTTTGTTCTCCCGACGTACTCTGCGTTGKAGGATTTATCCACCC
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00501 Ps_contig_mira-and-tgicl-ass_franssen_5118
SEQUENCE=TGACAAAGAACATTGGATCATAACAAACTAAACATATCAGTTTCAGATAAACATCAACAAAGACAGGGCCAACGTGACCTAGTCTAAACACCCAAGTAATAGCTTTCATCTCACTGCTGCTCTAGCACTCATTGATGTGTGATAATTTGTGCTTCTCTGCTTCCGGTAAGATGTATATACATCATCATACTGACTCACTTCATTTGGATCAGCCGTGGCCCCAAGTCCCAATCTTCCCGAACCACTTGATCCTGGCTTTCTGTCGACACTCCCATCAGCATAGGTCCCTGTTTCTGGATCAATTTGCTGCAAGTTTGGGGGTGGAGGTATGCAAGCACCTCCCTTTCGTACTTGAGATTCTCTCTCGTATTCCCCATCGTATTCATCATCATCAT
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00502 Ps_contig_mira-and-tgicl-ass_franssen_5123
SEQUENCE=AAAAGGAAGATTTGAAGCAGAACCTATGTGTTTTGTAGTAARATCATCTATACATATGTGTTCATCTTCCATATATTGTTTAGGCCCTCTCTCAGCACAACTTCCTGATCGAAATATGGGTAGATAATCTGTCTTGTCTTGAGAAGATGACTTACTTCCAGTTACCCCGGCATCTACACCCAAATTTGCTACAGCCTGCATCAATCTGGTTGAGCTAAGGCTGTGTCTCAAACCAGACACATGCCTTGGAGGTTTCCCCTTTGTGATTGGTTTCAGATTCTTAAAATCCTCTGATTTATCGTCATCATCATCATCAATAACATCAACATCATCATCATCATGAACAACAACCAGAAAATCATTGTTTTTATTGTAATCATCGCTTTCAAACATTGTAAATGGAGGAGAATAATAATCTACACCCTCAAGCATAATAGTTTCTTCCAAATCGTACACACCTAACTAACAAAAACCAATAAAAGAAGAGTCTTTTAAGGGAAAAAATGTTTCAATTGGAAGAATAAGTTAAACCGAAATCGATAAATAAAGTGCCTAACCGTGATAATTTAAGAAAGAGAAACAGATTGAAGATGGTGAATAGGAAGGAATTAGG
TARGET=302,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00503 Ps_contig_mira-and-tgicl-ass_franssen_5123
SEQUENCE=AAAAGGAAGATTTGAAGCAGAACCTATGTGTTTTGTAGTAARATCATCTATACATATGTGTTCATCTTCCATATATTGTTTAGGCCCTCTCTCAGCACAACTTCCTGATCGAAATATGGGTAGATAATCTGTCTTGTCTTGAGAAGATGACTTACTTCCAGTTACCCCGGCATCTACACCCAAATTTGCTACAGCCTGCATCAATCTGGTTGAGCTAAGGCTGTGTCTCAAACCAGACACATGCCTTGGAGGTTTCCCCTTTGTGATTGGTTTCAGATTCTTAAAATCCTCTGATTTATCGTCATCATCATCATCAATAACATCAACATCATCATCATCATGAACAACAACCAGAAAATCATTGTTTTTATTGTAATCATCGCTTTCAAACATTGTAAATGGAGGAGAATAATAATCTACACCCTCAAGCATAATAGTTTCTTCCAAATCGTACACACCTAACTAACAAAAACCAATAAAAGAAGAGTCTTTTAAGGGAAAAAATGTTTCAATTGGAAGAATAAGTTAAACCGAAATCGATAAATAAAGTGCCTAACCGTGATAATTTAAGAAAGAGAAACAGATTGAAGATGGTGAATAGGAAGGAATTAGG
TARGET=327,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00504 Ps_contig_mira-and-tgicl-ass_franssen_5127
SEQUENCE=GGTTCAATACTATAATTTTATTTATGAATAAAACACTAGTATAGTATAGTATCAAACTATGAATCAATATTATTACAACAACAAGAAGAACTTAACAAGTATTGAAACTCAAAATCAAAGACAAGCAGGACAATGAACTAACCCATTCTCATTACACTTAGGACACCTCTGCAGTGTCGAATCACTTATTTCAACCCCACTAATCACCAACTTACAGCTTCCACCACAATCCAAACAAGGAACAAACCTAGCCCCACCACACCCCCCACAACCTTGTCTCCCAACACCTCTCTCCACACGCGTCGCATTCAATATCCTCCCAAGTCTCCCCGTTTCATTTAACTCCGTCAACTCATCCAAACCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCCGACTCTTCCTCCGTTAACAACAACTCTTTCACTTCCTTCAAAAACTCTCCGTGAAGTGCCACGTCACGCTCGTCGAATATCACCCTGTGATTCTCTAACAAGTCCCGAGCTTTGTTGCAGTTATCGAAAGTTTTTCTAACTCCTCTTAACGACGTCGTGTATATCACCACGGCGTCGTTTCCTTTCGGTGGAACTTTCTCCGGGTAGTTACTCAGTGGTCCCTCCTTAACCGTTCGAAATTCGATTCTCTCTCTTCTTTTTGTTTCTCTAACATGTCTTTCTGTA
TARGET=37,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00505 Ps_contig_mira-and-tgicl-ass_franssen_5128
SEQUENCE=GACATGTACTACTACTACAACACKAGTATATGTGAGGGAGAGAGTCACTGCAACTAGCTATCATCCAAAGAACGAAATGGGGTGTGCATCTTCCAAAGGTATCGACGGCACGGCAGTCGCCGTATACCAGCCACCACCGACGAGCTTCGCCGTGTTCGACATAAACTCCATAGAAGAGCCATGGCTCAAACACCTCAACGACAACACCAACACCATGTCACAAAGAAAACAAACCCGCTCTCCCTCAACCTATTCTCCA
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00506 Ps_contig_mira-and-tgicl-ass_franssen_5137
SEQUENCE=TGCTTAAGATTACTAATAATCTCGACTTCTATTTACAAAACTCGACATCCCCTTGAAAATCGGATTCTAAAACCCGCTTAACCGCAACAACAGATCCATCAGCCAAAGTTCCTTTGAAAACCAAACCAAACCCACCTCTCCCTATGAAATGTCTTGGATGAAAAATTATCAGTTGCTTTCTCAAGTTCCGGAATTTTGTACCAAATCGACCCGGTATTCGGTCTCACCTACGGCGAGAGTCTTGTTCCTCGGGATCAAATCAATTGAAAGTTCAGCTTCTCTTCC
TARGET=118,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0421
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=1.037717
PRIMER_LEFT_SEQUENCE=AACAGATCCATCAGCCAAAGTT
PRIMER_RIGHT_SEQUENCE=CGGAACTTGAGAAAGCAACTG
PRIMER_LEFT=88,22
PRIMER_RIGHT=189,21
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=1.1663
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=1.037717
PRIMER_LEFT_1_SEQUENCE=TTTACAAAACTCGACATCCCCT
PRIMER_RIGHT_1_SEQUENCE=CGGAACTTGAGAAAGCAACTG
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=189,21
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=1.1663
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=1.037717
PRIMER_LEFT_2_SEQUENCE=TTACAAAACTCGACATCCCCTT
PRIMER_RIGHT_2_SEQUENCE=CGGAACTTGAGAAAGCAACTG
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=189,21
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.038
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=1.2252
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=1.096569
PRIMER_LEFT_3_SEQUENCE=TTACAAAACTCGACATCCCCTT
PRIMER_RIGHT_3_SEQUENCE=CATCCAAGACATTTCATAGGGA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=159,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=58.903
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=1.2252
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=1.096569
PRIMER_LEFT_4_SEQUENCE=TTACAAAACTCGACATCCCCTT
PRIMER_RIGHT_4_SEQUENCE=TCATCCAAGACATTTCATAGGG
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=160,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=58.903
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_00507 Ps_contig_mira-and-tgicl-ass_franssen_5147
SEQUENCE=CTTTTGATGCTAAGAGTTAAGCATGAATCTCTTGCCCAGACTCAAAGGAATTTTTTGGGAGAAGAACTCAATACACTAAGCATAAAAGATTTGCAGAATATAGAAAAACAACTGGAGAGAACGCTTGCACAAGCTCGAAAGCACCAAATGCAGAAACTGATGACACGAGTTGATGAATTAGGCCGAGAGGTTCACAAGGTGGAGGAGGTTAACAAACAGTTAGAATCCAAGTCAGTTTCGGCACCAACAAGCTGGTTCAAAAGGACGGGCAACATATACAAGGATTGACCGAGGCCAGAGTAGTCGCAACAAGAACAATGGATGGATGAATATTTGAACTTTCATTTACCTAAGGCTTGAGACCCCTAAAACATGTCTCAAATAATCACTAATTTATGAGAGCCTATTTGGTTTAATTTATAATAGAACTTCTTCTTTCTTTCTTTTCTWRCAYGAYARTRWSGCKTCARGTTGGGTCGGTTT
TARGET=433,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00508 Ps_contig_mira-and-tgicl-ass_franssen_5155
SEQUENCE=CAATCTATCAACCTGCAACAACAACAACAACAATAACGTGCAATAACATCATCATCATCAACATGAAAACAGAACATGCAAATGAATACGATGAGAATCAAGTCGAAGAAGAAACCAATTGATGATGAGAATCGAAGTTGACAAGATCGTGAGTAGGATGTGGCTGAATGACGAGTTCATCGGAAGGACGAAGGTGAACGGTGGCATAGAAATTGGAAAGACTGAAAGCGATTGTTTTGCCGTAATAGACATCGCTTGGATTTCCGAGAAAACCAA
TARGET=16,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00509 Ps_contig_mira-and-tgicl-ass_franssen_5155
SEQUENCE=CAATCTATCAACCTGCAACAACAACAACAACAATAACGTGCAATAACATCATCATCATCAACATGAAAACAGAACATGCAAATGAATACGATGAGAATCAAGTCGAAGAAGAAACCAATTGATGATGAGAATCGAAGTTGACAAGATCGTGAGTAGGATGTGGCTGAATGACGAGTTCATCGGAAGGACGAAGGTGAACGGTGGCATAGAAATTGGAAAGACTGAAAGCGATTGTTTTGCCGTAATAGACATCGCTTGGATTTCCGAGAAAACCAA
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0402
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=AACCTGCAACAACAACAACAAC
PRIMER_RIGHT_SEQUENCE=CGATGTCTATTACGGCAAAACA
PRIMER_LEFT=9,22
PRIMER_RIGHT=253,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.0777
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=ATCAACCTGCAACAACAACAAC
PRIMER_RIGHT_1_SEQUENCE=CGATGTCTATTACGGCAAAACA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1806
PRIMER_LEFT_2_PENALTY=0.019966
PRIMER_RIGHT_2_PENALTY=0.160609
PRIMER_LEFT_2_SEQUENCE=AACCTGCAACAACAACAACAAC
PRIMER_RIGHT_2_SEQUENCE=AGCCACATCCTACTCACGATCT
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=60.161
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.2180
PRIMER_LEFT_3_PENALTY=0.057411
PRIMER_RIGHT_3_PENALTY=0.160609
PRIMER_LEFT_3_SEQUENCE=ATCAACCTGCAACAACAACAAC
PRIMER_RIGHT_3_SEQUENCE=AGCCACATCCTACTCACGATCT
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=60.161
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.2520
PRIMER_LEFT_4_PENALTY=0.019966
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=AACCTGCAACAACAACAACAAC
PRIMER_RIGHT_4_SEQUENCE=AACAATCGCTTTCAGTCTTTCC
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_00510 Ps_contig_mira-and-tgicl-ass_franssen_5172
SEQUENCE=TTAATAATTTAAATTACTTTTTCTTTTAAAAACCCTAAAACCTTTCTTCCTAAATAAGCATCTAACACTACGCAAATACCCTACCCTTTCCAAAGACCTTCCTTCCCTTCATGAACTGAAAGAAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATCTTTCAAAATCTTTGACCAATCTGGTATATTCATCGGTAAAGAAGATGAACCTATAGGGTTAACTCGGTTATTGGATTCCATTTTTCTAGAAACTCTCTTCAAACCTGGTAGCACACACTTTTTTGATTCTGTCATGTTGTCGGAATTTGAACATGACATGTTCCATACATCAGCTTCATCGAGCTCGAACTCAGTTGCTTCGGATGATTTTGTGTTTATCTCTGAGGGTGTTGGAAAAATGTAACTTGATCTTGAAAGAAAACTCTTCCTAGAAGAAGCCATTGGAAGTGGTTTTGGTGTTTTTGTTTTTGTTTTTGTTTTTGTTACTATGTTTTCATTTTGTTCTGTTTTTTTGGCTTATATTGTACACTCTGGTCTCACTCTACGCGTTATGATAAATMAAHGAMACAAGTAATAATAGTAAATAC
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00511 Ps_contig_mira-and-tgicl-ass_franssen_5172
SEQUENCE=TTAATAATTTAAATTACTTTTTCTTTTAAAAACCCTAAAACCTTTCTTCCTAAATAAGCATCTAACACTACGCAAATACCCTACCCTTTCCAAAGACCTTCCTTCCCTTCATGAACTGAAAGAAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATCTTTCAAAATCTTTGACCAATCTGGTATATTCATCGGTAAAGAAGATGAACCTATAGGGTTAACTCGGTTATTGGATTCCATTTTTCTAGAAACTCTCTTCAAACCTGGTAGCACACACTTTTTTGATTCTGTCATGTTGTCGGAATTTGAACATGACATGTTCCATACATCAGCTTCATCGAGCTCGAACTCAGTTGCTTCGGATGATTTTGTGTTTATCTCTGAGGGTGTTGGAAAAATGTAACTTGATCTTGAAAGAAAACTCTTCCTAGAAGAAGCCATTGGAAGTGGTTTTGGTGTTTTTGTTTTTGTTTTTGTTTTTGTTACTATGTTTTCATTTTGTTCTGTTTTTTTGGCTTATATTGTACACTCTGGTCTCACTCTACGCGTTATGATAAATMAAHGAMACAAGTAATAATAGTAAATAC
TARGET=221,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00512 Ps_contig_mira-and-tgicl-ass_franssen_5173
SEQUENCE=GGGTTTTAGGGGGTAAACGGGGTTAAAAATTAAAACCGTAAAGGTTAACGGGTTTAACGGTTAAACTTTTACCTTGGTAAATTAAGTTAGAGTTGTTTACCAAACCCGTAACCGTTTAGTTATCTAGTTAGAAATAATAATTAATTAATTCTTACCAAAACCTAACTTTCCTTACCTAAATAGMATTCCTAACACTACGCAAATCCCTTACCTTTCTAAAGTCCTTCCTTTTCCCTTTCATGAACTGAAAGAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATTCTTTACAAATTCTTGACCATCTGTATATTCATACGGTAAAAGAAGATAGAACCTTAAGTTAGGGGTTAACTTCGGTTAG
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00513 Ps_contig_mira-and-tgicl-ass_franssen_5173
SEQUENCE=GGGTTTTAGGGGGTAAACGGGGTTAAAAATTAAAACCGTAAAGGTTAACGGGTTTAACGGTTAAACTTTTACCTTGGTAAATTAAGTTAGAGTTGTTTACCAAACCCGTAACCGTTTAGTTATCTAGTTAGAAATAATAATTAATTAATTCTTACCAAAACCTAACTTTCCTTACCTAAATAGMATTCCTAACACTACGCAAATCCCTTACCTTTCTAAAGTCCTTCCTTTTCCCTTTCATGAACTGAAAGAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATTCTTTACAAATTCTTGACCATCTGTATATTCATACGGTAAAAGAAGATAGAACCTTAAGTTAGGGGTTAACTTCGGTTAG
TARGET=326,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00514 Ps_contig_mira-and-tgicl-ass_franssen_5173
SEQUENCE=GGGTTTTAGGGGGTAAACGGGGTTAAAAATTAAAACCGTAAAGGTTAACGGGTTTAACGGTTAAACTTTTACCTTGGTAAATTAAGTTAGAGTTGTTTACCAAACCCGTAACCGTTTAGTTATCTAGTTAGAAATAATAATTAATTAATTCTTACCAAAACCTAACTTTCCTTACCTAAATAGMATTCCTAACACTACGCAAATCCCTTACCTTTCTAAAGTCCTTCCTTTTCCCTTTCATGAACTGAAAGAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATTCTTTACAAATTCTTGACCATCTGTATATTCATACGGTAAAAGAAGATAGAACCTTAAGTTAGGGGTTAACTTCGGTTAG
TARGET=350,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00515 Ps_contig_mira-and-tgicl-ass_franssen_5178
SEQUENCE=GKTCTTAATTTAATCATGTAATTAAGAAAAGCCAGAAAGATAGAAACATAATAATTCTGAAAGATCATCCATATATTCGGTTGGTGCGAACAATGATCATTCAGAAAACTCATAATTCAAGCATCACGAAAAGTGATATGGAGGTAGAGAATTCAACATTATGTTGATCGATTCGATTGATTAATTCACATATACGATTCAGATCCAATATTTCACATCTTATACAAAGTGAAATAGCTATATATATAATTAACATATAAAAGAACAAAAAGAAAGAAAGAAATGGATCATGATGATGAAAGAAGAGGAAATCGATAAAAGAAGAAAAATCGTGTAATTAATTTACGTAATTTAACCAACGAGCACCAAGGCAGTGTTCGAGACTGAATTTCAGAGAGAGTTTGATCAATTTTCATGTACAAGGACAAGGCCTCTTTGGAAAAGTGGATCTGAAAAACGCATCCATGTCTTCATTACTCGAAACCTCGAAGAATTGAAATTCATCCATAAGCTTCCTCTTACAAGAAACGAACGGCTTCGGTTTCTTCGGCGCCGGCGGACAATTCAAGATCTCCGGTATCTTGCTTTCCTTCGAAGTCGGAGTCCGGTAASTCKGAGTCCG
TARGET=239,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00516 Ps_contig_mira-and-tgicl-ass_franssen_5178
SEQUENCE=GKTCTTAATTTAATCATGTAATTAAGAAAAGCCAGAAAGATAGAAACATAATAATTCTGAAAGATCATCCATATATTCGGTTGGTGCGAACAATGATCATTCAGAAAACTCATAATTCAAGCATCACGAAAAGTGATATGGAGGTAGAGAATTCAACATTATGTTGATCGATTCGATTGATTAATTCACATATACGATTCAGATCCAATATTTCACATCTTATACAAAGTGAAATAGCTATATATATAATTAACATATAAAAGAACAAAAAGAAAGAAAGAAATGGATCATGATGATGAAAGAAGAGGAAATCGATAAAAGAAGAAAAATCGTGTAATTAATTTACGTAATTTAACCAACGAGCACCAAGGCAGTGTTCGAGACTGAATTTCAGAGAGAGTTTGATCAATTTTCATGTACAAGGACAAGGCCTCTTTGGAAAAGTGGATCTGAAAAACGCATCCATGTCTTCATTACTCGAAACCTCGAAGAATTGAAATTCATCCATAAGCTTCCTCTTACAAGAAACGAACGGCTTCGGTTTCTTCGGCGCCGGCGGACAATTCAAGATCTCCGGTATCTTGCTTTCCTTCGAAGTCGGAGTCCGGTAASTCKGAGTCCG
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00517 Ps_contig_mira-and-tgicl-ass_franssen_5180
SEQUENCE=AAGACAGTGGTATCAACGCAGAGAACCTTCCTTTCCCTCTGTTTTTGTCATCAAAATTAAAGTCATTGTGGTGTTTGTTTGTTTAAAGTCAGTCGTTTAAAATTTCTCATTGTGAAAAACTAAA
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00518 Ps_contig_mira-and-tgicl-ass_franssen_5181
SEQUENCE=CGTCTCGGCTTTCTCCCAAGAAAGACGAGCTGCTCGTCATCGCGGAAAAGTAGAAAAGACCTTTTCCCTAAAACGAATGATCCTACCTAAACCATGCAGCTTGACTGCTTTTGTTGGCTACAAGTCTGGGATGACCCATATTGTTAGGGAGGTTGAGAAGCCTGGATCCAAGCTTCATAAGAAAGAGACATGTGAAGCTGTCACTATCATTGAAACTCCTCCACTTGTTGTTGTTGGAGTGGTGGGATATGTGAAAACACCGCGAGGCCTTCGCACACTGAATACTGTTTGGGCTCAGCATCTAAGTGAAGATATCAAGCGTCGGTTTTACAAGAACTGGTGCAAGTCAAAGAAGAAGGCTTTTACCAAGTATTCCAAAAAGTATGAAACTGAAGAAGGAAAGAAGGATATTCAATCTCAGCTTGAGAAATTGAAAAAATATGCTACTGTCATCCGTGTTCTTGCTCATACTCAGATAAGGAAGTTGAAGGGTCTAAAACAAAAGAAAGCTCATTTGATAGAGATCCAAGTTAATGGTGGAACAATAAGCCAAAAGGTGGATTATTCATACAGTTTCTTTGAAAAGCAAGTTCCTGTCAATGCTGTTTTTCAGAAGGATGAGATGATTGATATCATTGGAGTAACTAAAGGTAAAGGTTATGAAGGTGTGGTAACTCGTTGGGGTGTGACCCGCCTTCCACGGAAGACACACAGAGGTCTAAGGAAGGTTGCTTGTATTGGTGCTTGGCACCCTGCTCGTGTTTCTTTCACAGTTGCCAGGGCTGGTCAGAATGGATACCATCATCGTACTGAATTGAACAAGAAGATCTACAAGGTTGGCAAGACTGACGAAGAGTCACACACAGCCATCACAGAATTTGACAGGACTGAGAAGGATATTACTCCCATGGGTGGATTTCCTCATTACGGTATAGTGAAGCATGACTATCTTATGGTGAAGGGAGGCTGTGTTGGACCAAAGAAGCGGGTTGTGACTCTTCGTCAATCACTACTGAAGCAAACGTCTCGATTGGCTCTTGAAGAGATCAAGTTGAAATTCATTGACACCTCCTCCAAGTTTGGACATGGTCGCTTCCAGACTACTCAGGAGAAACAGAAGTTTTATGGTCGTTTGAAGGCCTAGTTCTTATTTTATTATATTTATTGTTTATTGTTTATTGTGTCGAGAGAACCTTCCTTTTCCCTCTGTTTTTGCATCAAATTAAGTCATTGTGGTGTTTGTTTGTTTAAAGTCAGTCGTTTAAATTTCTCATTGTGAAAAACTAATTTTGTTTTACTTTGATGGGTTTGTGTATTTGCCTT
TARGET=225,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0556
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.048692
PRIMER_LEFT_SEQUENCE=GATCCTACCTAAACCATGCAGC
PRIMER_RIGHT_SEQUENCE=CTTTGACTTGCACCAGTTCTTG
PRIMER_LEFT=78,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.951
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0678
PRIMER_LEFT_1_PENALTY=0.006951
PRIMER_RIGHT_1_PENALTY=0.060824
PRIMER_LEFT_1_SEQUENCE=GATCCTACCTAAACCATGCAGC
PRIMER_RIGHT_1_SEQUENCE=AGCCTTCTTCTTTGACTTGCAC
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=360,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.1025
PRIMER_LEFT_2_PENALTY=0.006951
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=GATCCTACCTAAACCATGCAGC
PRIMER_RIGHT_2_SEQUENCE=TAGATGCTGAGCCCAAACAGTA
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.1170
PRIMER_LEFT_3_PENALTY=0.068302
PRIMER_RIGHT_3_PENALTY=0.048692
PRIMER_LEFT_3_SEQUENCE=CCCATATTGTTAGGGAGGTTGA
PRIMER_RIGHT_3_SEQUENCE=CTTTGACTTGCACCAGTTCTTG
PRIMER_LEFT_3=134,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=60.068
PRIMER_RIGHT_3_TM=59.951
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.1291
PRIMER_LEFT_4_PENALTY=0.068302
PRIMER_RIGHT_4_PENALTY=0.060824
PRIMER_LEFT_4_SEQUENCE=CCCATATTGTTAGGGAGGTTGA
PRIMER_RIGHT_4_SEQUENCE=AGCCTTCTTCTTTGACTTGCAC
PRIMER_LEFT_4=134,22
PRIMER_RIGHT_4=360,22
PRIMER_LEFT_4_TM=60.068
PRIMER_RIGHT_4_TM=60.061
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_00519 Ps_contig_mira-and-tgicl-ass_franssen_5181
SEQUENCE=CGTCTCGGCTTTCTCCCAAGAAAGACGAGCTGCTCGTCATCGCGGAAAAGTAGAAAAGACCTTTTCCCTAAAACGAATGATCCTACCTAAACCATGCAGCTTGACTGCTTTTGTTGGCTACAAGTCTGGGATGACCCATATTGTTAGGGAGGTTGAGAAGCCTGGATCCAAGCTTCATAAGAAAGAGACATGTGAAGCTGTCACTATCATTGAAACTCCTCCACTTGTTGTTGTTGGAGTGGTGGGATATGTGAAAACACCGCGAGGCCTTCGCACACTGAATACTGTTTGGGCTCAGCATCTAAGTGAAGATATCAAGCGTCGGTTTTACAAGAACTGGTGCAAGTCAAAGAAGAAGGCTTTTACCAAGTATTCCAAAAAGTATGAAACTGAAGAAGGAAAGAAGGATATTCAATCTCAGCTTGAGAAATTGAAAAAATATGCTACTGTCATCCGTGTTCTTGCTCATACTCAGATAAGGAAGTTGAAGGGTCTAAAACAAAAGAAAGCTCATTTGATAGAGATCCAAGTTAATGGTGGAACAATAAGCCAAAAGGTGGATTATTCATACAGTTTCTTTGAAAAGCAAGTTCCTGTCAATGCTGTTTTTCAGAAGGATGAGATGATTGATATCATTGGAGTAACTAAAGGTAAAGGTTATGAAGGTGTGGTAACTCGTTGGGGTGTGACCCGCCTTCCACGGAAGACACACAGAGGTCTAAGGAAGGTTGCTTGTATTGGTGCTTGGCACCCTGCTCGTGTTTCTTTCACAGTTGCCAGGGCTGGTCAGAATGGATACCATCATCGTACTGAATTGAACAAGAAGATCTACAAGGTTGGCAAGACTGACGAAGAGTCACACACAGCCATCACAGAATTTGACAGGACTGAGAAGGATATTACTCCCATGGGTGGATTTCCTCATTACGGTATAGTGAAGCATGACTATCTTATGGTGAAGGGAGGCTGTGTTGGACCAAAGAAGCGGGTTGTGACTCTTCGTCAATCACTACTGAAGCAAACGTCTCGATTGGCTCTTGAAGAGATCAAGTTGAAATTCATTGACACCTCCTCCAAGTTTGGACATGGTCGCTTCCAGACTACTCAGGAGAAACAGAAGTTTTATGGTCGTTTGAAGGCCTAGTTCTTATTTTATTATATTTATTGTTTATTGTTTATTGTGTCGAGAGAACCTTCCTTTTCCCTCTGTTTTTGCATCAAATTAAGTCATTGTGGTGTTTGTTTGTTTAAAGTCAGTCGTTTAAATTTCTCATTGTGAAAAACTAATTTTGTTTTACTTTGATGGGTTTGTGTATTTGCCTT
TARGET=1237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1341
PRIMER_LEFT_PENALTY=0.018372
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=GAACCTTCCTTTTCCCTCTGTT
PRIMER_RIGHT_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT=1189,22
PRIMER_RIGHT=1322,22
PRIMER_LEFT_TM=59.982
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.1368
PRIMER_LEFT_1_PENALTY=0.021044
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=CCTTCCTTTTCCCTCTGTTTTT
PRIMER_RIGHT_1_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_1=1192,22
PRIMER_RIGHT_1=1322,22
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.1370
PRIMER_LEFT_2_PENALTY=0.021195
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=TATGGTCGTTTGAAGGCCTAGT
PRIMER_RIGHT_2_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_2=1123,22
PRIMER_RIGHT_2=1322,22
PRIMER_LEFT_2_TM=60.021
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.2247
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=CTTTTCCCTCTGTTTTTGCATC
PRIMER_RIGHT_3_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_3=1197,22
PRIMER_RIGHT_3=1322,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.2518
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=TGTCGAGAGAACCTTCCTTTTC
PRIMER_RIGHT_4_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_4=1181,22
PRIMER_RIGHT_4=1322,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_00520 Ps_contig_mira-and-tgicl-ass_franssen_5195
SEQUENCE=TCTACTTTCTGGAAGTTTTTGACGCACGAAACGACGCGATCTGTGACCTCAGATTTATCGAGGAAAGAACCCCTAACCTCTTCGCTGAAGCGACGGAGGAAGAAATTGGGAAGAAGACGCAGAGGAGACTGGTGGTTGAGCGGAAGAGTATCAACCTGAACTCTTAGGTATTTCATCAAAGGCAAACCGGTTCTCCTCGCCGCCATTGCTGATATCAAAAACCTCACTCTCTCTCTCTCTCTCTGTTCAAATCTACACAACGAAGTGAAAAGAAATATTGTGAAGTTAGATCCMRTGATACCCAAACCAAACCTTGTTTTTAACACTGGGCTTTGTTTACTCC
TARGET=227,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00521 Ps_contig_mira-and-tgicl-ass_franssen_5196
SEQUENCE=GATCTAACTTCGACAATATTTCTTTTCACTTCGTTGTGTAGATTTGAACAGAGAGAGAGAGAGTGAGGTTTTTGATATCAGCAATGGCGGCGAAGGAGAACCGGTTT
TARGET=50,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00522 Ps_contig_mira-and-tgicl-ass_franssen_5197
SEQUENCE=CGGGGACAGAATSAGATCTAAAACTTTTGTTGTTGTTTTGTTTTTCTATATGTCTGATTTCCTATGTTTCTTCATTCATTCATGTTTCTTCAACTTGTCTGTCATTTCACCTTTCTTTCCTAAACCCCTAACTCTACACTCTCTATTTTCTCACACCNTGCACACCCAACCAATAACTACACCAACAAGAATCTTTGAGTACCTTTCCCAATACTAAATTTCAATCCGTTGGGTTTTTCTCATTCAACCATAAACG
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00523 Ps_contig_mira-and-tgicl-ass_franssen_5199
SEQUENCE=CAAAGGTGTCTTCATCGGACTAAACTGAGGGAATATATCTGATCTTCGTCCTGGCTTAGGCCTCTCCCACTCGATTGGGAAATCGAGAGGCGAACGACGGACTAACTTCTCGTCTTCAGGAGCCATACGAACAACATGCCTCGAAAAACGCGAACGGGAAGAGATAGAGATGATAGACGGTAATCGATAGTTACAAGAAGAAGAAGAAGGAGGATTAGGTTTAAGCGAAGAAGAGAGAGGAGTGAGAATAGGGTTTAGGGTGAAGAAAGCCATTCTTGTTTTTCTTCAAGTCTTGACGCACCTAATCCTATCCCAACCTTTCGTTCCCTTTTCTTATGTTCCCCGAA
TARGET=195,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0322
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_RIGHT_SEQUENCE=GGATAGGATTAGGTGCGTCAAG
PRIMER_LEFT=102,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.0703
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_RIGHT_1_SEQUENCE=GGATAGGATTAGGTGCGTCAAG
PRIMER_LEFT_1=94,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.0750
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=0.056142
PRIMER_LEFT_2_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_RIGHT_2_SEQUENCE=GGGGAACATAAGAAAAGGGAAC
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=60.056
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.0946
PRIMER_LEFT_3_PENALTY=0.018835
PRIMER_RIGHT_3_PENALTY=0.075753
PRIMER_LEFT_3_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_RIGHT_3_SEQUENCE=AACGAAAGGTTGGGATAGGATT
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=60.076
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.1130
PRIMER_LEFT_4_PENALTY=0.056850
PRIMER_RIGHT_4_PENALTY=0.056142
PRIMER_LEFT_4_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_RIGHT_4_SEQUENCE=GGGGAACATAAGAAAAGGGAAC
PRIMER_LEFT_4=94,22
PRIMER_RIGHT_4=343,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.056
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_00524 Ps_contig_mira-and-tgicl-ass_franssen_5200
SEQUENCE=GTCGGGGGGGTCGGGGTAGGTGCGTCAAGACTTGAAGAAAAACAAGAATGGCTTTCTTCACCCTAAACCCTATTCTCACTCCTCTCTCTTCTTCGCTTAAACCTAATCCTCCTTCTTCTTCTTGTAACTATCGATTACCGTCTATCATCTCTATCTCTTCCCGTTCGCGTTTTTCGAGGCATGTTGTTCGTATGGCTCCTGAAGACGAGAAGTTAGTCCGTCGTTCGCCTCTCGATTTCCCAATCGAGTGGGAGAAGGCCTAA
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1183
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=TCACTCCTCTCTCTTCTTCGCT
PRIMER_RIGHT_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_LEFT=75,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=0.1183
PRIMER_LEFT_1_PENALTY=0.099438
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=CTCACTCCTCTCTCTTCTTCGC
PRIMER_RIGHT_1_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.1563
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=TCACTCCTCTCTCTTCTTCGCT
PRIMER_RIGHT_2_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=222,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.1563
PRIMER_LEFT_3_PENALTY=0.099438
PRIMER_RIGHT_3_PENALTY=0.056850
PRIMER_LEFT_3_SEQUENCE=CTCACTCCTCTCTCTTCTTCGC
PRIMER_RIGHT_3_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=222,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.2830
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.183585
PRIMER_LEFT_4_SEQUENCE=TCACTCCTCTCTCTTCTTCGCT
PRIMER_RIGHT_4_SEQUENCE=AACGACGGACTAACTTCTCGTC
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=224,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.816
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_00525 Ps_contig_mira-and-tgicl-ass_franssen_5208
SEQUENCE=CTAATAAACCACACATTTATTATAGAAGATAACAACACAAACACAAACAACCTTGCCGTTGATGGAAAATTGTCTTCACCACCTTACAATCAAAGGATTCTTCTTCACAAAACCTATTTCTTTTTCATCTTCTTCTTCTTCTTCTTCTTCTTTGAAATTTAGGTGCAACAACAACAGCACCAAATTGAAGGTGATTAAGGCGAGTGCCGGAGCTAGCCACTGTCAGTTCAGTAGTTTGAACTCTCCTTTGTACCACAATCCRCGGTTGGTAAA
TARGET=128,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00526 Ps_contig_mira-and-tgicl-ass_franssen_5216
SEQUENCE=AATAATGGCTGTGGCTATGGCTGCTTGTAGCCTTGGACTTCACCTCACTCCTGATAATAATAATAATAATAATAATACTAATTGCTTTGCCCCTATCTTGAAAACCACTGCTATGTATGATACTAGTAAACCATGTTTCATTCTTCACAAAGCAAGATTATTATCTTCAGCAATTACGATCGTCCCTCGAGCAACAGCTTGTTTCTAATGTCGAGG
TARGET=54,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1336
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.959092
PRIMER_LEFT_SEQUENCE=GTAGCCTTGGACTTCACCTCAC
PRIMER_RIGHT_SEQUENCE=CCTCGACATTAGAAACAAGCTG
PRIMER_LEFT=26,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=59.041
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=1.1486
PRIMER_LEFT_1_PENALTY=0.189529
PRIMER_RIGHT_1_PENALTY=0.959092
PRIMER_LEFT_1_SEQUENCE=CTTGTAGCCTTGGACTTCACCT
PRIMER_RIGHT_1_SEQUENCE=CCTCGACATTAGAAACAAGCTG
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=59.810
PRIMER_RIGHT_1_TM=59.041
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=1.2610
PRIMER_LEFT_2_PENALTY=0.301926
PRIMER_RIGHT_2_PENALTY=0.959092
PRIMER_LEFT_2_SEQUENCE=TAGCCTTGGACTTCACCTCACT
PRIMER_RIGHT_2_SEQUENCE=CCTCGACATTAGAAACAAGCTG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=215,22
PRIMER_LEFT_2_TM=60.302
PRIMER_RIGHT_2_TM=59.041
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=1.4528
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=1.278288
PRIMER_LEFT_3_SEQUENCE=GTAGCCTTGGACTTCACCTCAC
PRIMER_RIGHT_3_SEQUENCE=GGACGATCGTAATTGCTGAAG
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=184,21
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=59.722
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=1.5802
PRIMER_LEFT_4_PENALTY=0.301926
PRIMER_RIGHT_4_PENALTY=1.278288
PRIMER_LEFT_4_SEQUENCE=TAGCCTTGGACTTCACCTCACT
PRIMER_RIGHT_4_SEQUENCE=GGACGATCGTAATTGCTGAAG
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=184,21
PRIMER_LEFT_4_TM=60.302
PRIMER_RIGHT_4_TM=59.722
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_00527 Ps_contig_mira-and-tgicl-ass_franssen_5217
SEQUENCE=TCGGGGATATAATCTCAACCAAACGCGWCACTCTTTTATTAGTTTTTCAAGATTAGAAGTGAACGTCACAGATTTATCTCCTATTTTCTAGATAAATTCACTCATTCATTCATTCTTCTCTCTCAAAAATCCCTTTATCCTCCTTCAACTCTTTCAATAATGGCTGTGGCTATGGCTGCTTGTAGCCTAGGACTTCACCTCACTCCTGATAATAATAATAATAATAATACTAATTGWCTTTCGCCCCTATCTTAGAAAACCACTGCTATGTATGATACTAACGTAAACC
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00528 Ps_contig_mira-and-tgicl-ass_franssen_5217
SEQUENCE=TCGGGGATATAATCTCAACCAAACGCGWCACTCTTTTATTAGTTTTTCAAGATTAGAAGTGAACGTCACAGATTTATCTCCTATTTTCTAGATAAATTCACTCATTCATTCATTCTTCTCTCTCAAAAATCCCTTTATCCTCCTTCAACTCTTTCAATAATGGCTGTGGCTATGGCTGCTTGTAGCCTAGGACTTCACCTCACTCCTGATAATAATAATAATAATAATACTAATTGWCTTTCGCCCCTATCTTAGAAAACCACTGCTATGTATGATACTAACGTAAACC
TARGET=209,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00529 Ps_contig_mira-and-tgicl-ass_franssen_5222
SEQUENCE=TTTTTTTGTAGGGAAGAGTGATCGTTTGCAGAGTTATTTGTAGCCATAGCTTCTGAGGCAGCGAAACAGAGTTTGAAAAAGAAGGGTTTGCATGTTCCACCATGGAGAAAACTCGAGTACGTTAGATCAAAATGGTTATCTCCTTACACAAGAACCAAACAACCTCTGTTTGCAATTGGCCTCGTGAAATCAGTGAGGAAACTGAGAAGAAAACAGGTAGGGGTTTTTGATTGGGTGGTGGTGGTGGTGAGTCTGAAGTGAGTCGNGA
TARGET=234,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00530 Ps_contig_mira-and-tgicl-ass_franssen_5223
SEQUENCE=AGGAAAACAAGAGGGTTTGGATTGGTGGTGGTGGTGGTGGAGGTCTGAAGTGAGTCGCGATGAGTCAGAGTCAAAAGCGGTGGTTGAATGGAAGCCACCGGAATTGAAACCCAAAAACTCGCTGACCGGGGTTAAGGTTGTGACTGGTTTGGCAGCTGTCTTTCGTGAAAACCCATGAAATTTTGTTTTTTAACCCAAAAAAAAAGAAGTCAGGTTTTTGTATTTTAATATGAGTATTTTAATTCTCTATATAGATAGAGAATTACAAGAGCTTGTGTAAGTACTGTAATAGGCTAACAGCTACTAACCCCTTTSTTAKTTTWWTAAAAGKTAAAAAAAAGG
TARGET=23,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00531 Ps_contig_mira-and-tgicl-ass_franssen_5245
SEQUENCE=CGTTTTTTTAGTAAGAGAGTTTCAAATTGCCTTTGAAGGTAAAAAACCTCACACCATACAATCCAAGTCTTTTCATTATATATACAAGCTAACAGCATGACATAATTTTATTTATGCACATTGAAGGAAATAGAATAAAGCATTCTACATAGTTTACTCATTGTGCTTCCGCGACAGAGTAATGCATTTCTGTAACTGATTTTTTCAAGAAACTAGCTCCGCAGTTTGAATCTTGGCTACAAACATCAACAGAGGCAAAATCAGAAGAACCTTCAATAACTTGAGAATCCGACAACTCATAGCACAGTTCAGAATGTTCACCAAGGTTGCGAGACTGTTTCTTATCTTTATCTTTGCCATTGCTCCCTCGAGTTGAATTTCCCTTGTTACCTTTCTTCAAACTTTTCTTTTCATTCTTCTTCTTCTTGGATTTCGGCTGTTTCTTGTTTTTGACAGTATGCGACACTAATGTAGGTATTGGATCATACACATCAGCAGCCC
TARGET=414,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0862
PRIMER_LEFT_PENALTY=0.023406
PRIMER_RIGHT_PENALTY=1.062826
PRIMER_LEFT_SEQUENCE=TTTTTCAAGAAACTAGCTCCGC
PRIMER_RIGHT_SEQUENCE=GCTGCTGATGTGTATGATCCAA
PRIMER_LEFT=200,22
PRIMER_RIGHT=498,22
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=61.063
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=1.0960
PRIMER_LEFT_1_PENALTY=0.023406
PRIMER_RIGHT_1_PENALTY=1.072607
PRIMER_LEFT_1_SEQUENCE=TTTTTCAAGAAACTAGCTCCGC
PRIMER_RIGHT_1_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_1=200,22
PRIMER_RIGHT_1=455,22
PRIMER_LEFT_1_TM=60.023
PRIMER_RIGHT_1_TM=58.927
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.1142
PRIMER_LEFT_2_PENALTY=0.041553
PRIMER_RIGHT_2_PENALTY=1.072607
PRIMER_LEFT_2_SEQUENCE=CTAGCTCCGCAGTTTGAATCTT
PRIMER_RIGHT_2_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=455,22
PRIMER_LEFT_2_TM=60.042
PRIMER_RIGHT_2_TM=58.927
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=1.1535
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=1.072607
PRIMER_LEFT_3_SEQUENCE=AACTAGCTCCGCAGTTTGAATC
PRIMER_RIGHT_3_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_3=210,22
PRIMER_RIGHT_3=455,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=58.927
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=1.2124
PRIMER_LEFT_4_PENALTY=0.139753
PRIMER_RIGHT_4_PENALTY=1.072607
PRIMER_LEFT_4_SEQUENCE=GAATCCGACAACTCATAGCACA
PRIMER_RIGHT_4_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=455,22
PRIMER_LEFT_4_TM=60.140
PRIMER_RIGHT_4_TM=58.927
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_00532 Ps_contig_mira-and-tgicl-ass_franssen_5250
SEQUENCE=GAATAATTGACCTGTTTTTTCTTAATTAGTGTGTTTAATGYAATTCATTTTAATTGGGGAAATGGTTGCTTGTGTATGCACTTTGAATTTTTTTTTTTTGGCTGAAGCACTTTCAGTTATGTATGATGCTCAAAAGTCCAAGAAATATATATATTTGTAGTTTTAAC
TARGET=145,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00533 Ps_contig_mira-and-tgicl-ass_franssen_5251
SEQUENCE=TATTCACTTTGTTGATATGACCGAATGTTGAAAGGATCATCTTGGACCTGAGRGTTCATCTGGCTGGATATGTGAAGTTGAATCGACTATCATCCAATTTGCATTAGGGTGATGCATGTGGTTGTTGTTAAAATCTTGTCGATACGTCTTTTCTTTCTGCCATTGCTCCCATTTTTCTGCCAAACCATCACCTTCTAGCATTCTCACCACCTCAGACATCTTTGGTCTTTCCATCGGCGACCCTTGTGTGCATAGTAAAGCCACTTGGATTAACTTCTCTACCTCATCATCATCATAATTTCCCGTTAATTCTGCATCTACAAGTGTTTCCAACTTCTTGTCTTTGAGAAGTCCTTTAACCCAAT
TARGET=284,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00534 Ps_contig_mira-and-tgicl-ass_franssen_5257
SEQUENCE=TAAAAAAAGGAATTAAAGATGAACCACTGTTCATCTTCCTAACAACAACCCGTAACCACCGCGAAACCCATCATGAAACCTCCGTTTCCTCCTTTTCACACCTCCTTCGCTCCGCCGCATCAACCTTCTTCAAACACACACATTACCGGCGAACCGACCCCTCTCCGAACACAACAACCATAACTCCATAAATTTTCGTCGCCGCCGCGAAAAACTTCTGCAACAAAATATCAACCGCGCATCACCGACTCCTTCTGCCGTGATTCACAACCGATGACTCCTTTTTCTCCACATCCTCCGTCTACGCGCATATCCAACACCTTGCATCCACTACAATCCCACACGGCCAAGATATCGTACCACCATCTCCGAACTCAATCTCCAAGGTGGTTTTCTTTTTAACTTTAAAATTTGAGTTGTTTTGTGATTTGTTTTGTTTATGTTTTAATTATTCTTCTTTGATTTGGAATTGGAGAGAGATTGGATGAAACAAAGTATGGATTTTGTTTTATTTCAAATGATCATTGTTGTTGTTGATTGCTGTTGAGAGAAAGGAGAGTGTGAGTTTTTTTATTCTTTTTTTTCCGTTTGAAATAAAAAGAAGAAGCTTTGTTCTTCTTTATTTTTCTGAAAGAAAGATCTGTTGCATTGGTGGTTTCATTTTAAAGATGATGTTGTTTCTCATTTATTAGTTTTATTTGTGAAATTTTAAAATAGTTGAAAAAATCTACTAGATTTCATTTTACTGTTTTTTATAAATAAGAGGAGCATATACGGTTTTCTTGAAGGAGGAGAAATGTGTGGAAATAGAGTTTGTTCATTTTAGATCTAGGTTTCTTTCTTTAATAATGGACAATAATATTATTTAGTAATGTTTGAAAAGGTCATAATCATGTTATTTACTGTTTTTGAAGAGAGATGGAAACATGGGAGAGAGCTTTGTGGGGAAGAAACTTTTCTTTAATTTCATGTTTAGAAGGGTGATTTGAGTGAAATAGAGGATGTTGGTTTCTTAGATTTTTGTTTCTTTCTCTAAATTGCCAAGTGATAATTTGGATATGGCTAATGTTTTTTTTGAGAAAAGTAATGCTAGGCCTTAGGGGCGTCGCCCCAATTGTATATATTAAATTCTTGCTCATTTATTTTATTTATCTATTTATTTATTTTACTCATTAAGGTTTAATTTTAAATTATGGTTAGTGTTAATTATTGGGTTGGATTCAATTTATTTTTTACACCCCATGGTCATGTATACACTCTTACTATTTCTTTGTACTATTTTCTYTCTTGATTCGACTAATAGTAAATTAATTGATTAAGTTTTTTT
TARGET=525,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00535 Ps_contig_mira-and-tgicl-ass_franssen_5257
SEQUENCE=TAAAAAAAGGAATTAAAGATGAACCACTGTTCATCTTCCTAACAACAACCCGTAACCACCGCGAAACCCATCATGAAACCTCCGTTTCCTCCTTTTCACACCTCCTTCGCTCCGCCGCATCAACCTTCTTCAAACACACACATTACCGGCGAACCGACCCCTCTCCGAACACAACAACCATAACTCCATAAATTTTCGTCGCCGCCGCGAAAAACTTCTGCAACAAAATATCAACCGCGCATCACCGACTCCTTCTGCCGTGATTCACAACCGATGACTCCTTTTTCTCCACATCCTCCGTCTACGCGCATATCCAACACCTTGCATCCACTACAATCCCACACGGCCAAGATATCGTACCACCATCTCCGAACTCAATCTCCAAGGTGGTTTTCTTTTTAACTTTAAAATTTGAGTTGTTTTGTGATTTGTTTTGTTTATGTTTTAATTATTCTTCTTTGATTTGGAATTGGAGAGAGATTGGATGAAACAAAGTATGGATTTTGTTTTATTTCAAATGATCATTGTTGTTGTTGATTGCTGTTGAGAGAAAGGAGAGTGTGAGTTTTTTTATTCTTTTTTTTCCGTTTGAAATAAAAAGAAGAAGCTTTGTTCTTCTTTATTTTTCTGAAAGAAAGATCTGTTGCATTGGTGGTTTCATTTTAAAGATGATGTTGTTTCTCATTTATTAGTTTTATTTGTGAAATTTTAAAATAGTTGAAAAAATCTACTAGATTTCATTTTACTGTTTTTTATAAATAAGAGGAGCATATACGGTTTTCTTGAAGGAGGAGAAATGTGTGGAAATAGAGTTTGTTCATTTTAGATCTAGGTTTCTTTCTTTAATAATGGACAATAATATTATTTAGTAATGTTTGAAAAGGTCATAATCATGTTATTTACTGTTTTTGAAGAGAGATGGAAACATGGGAGAGAGCTTTGTGGGGAAGAAACTTTTCTTTAATTTCATGTTTAGAAGGGTGATTTGAGTGAAATAGAGGATGTTGGTTTCTTAGATTTTTGTTTCTTTCTCTAAATTGCCAAGTGATAATTTGGATATGGCTAATGTTTTTTTTGAGAAAAGTAATGCTAGGCCTTAGGGGCGTCGCCCCAATTGTATATATTAAATTCTTGCTCATTTATTTTATTTATCTATTTATTTATTTTACTCATTAAGGTTTAATTTTAAATTATGGTTAGTGTTAATTATTGGGTTGGATTCAATTTATTTTTTACACCCCATGGTCATGTATACACTCTTACTATTTCTTTGTACTATTTTCTYTCTTGATTCGACTAATAGTAAATTAATTGATTAAGTTTTTTT
TARGET=1154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00536 Ps_contig_mira-and-tgicl-ass_franssen_5261
SEQUENCE=AGTGAAATAATATGGCTGAAGTTATTATATAGATCCTTCTAGTTAAAGCTGCGTGATTTTATAATCTTTAGTGATTAATTTGATTATTATATTGTTTAATAATTTATGTGTTTAATTAATTCTTTATAGCCTTTAGGCAATAATTGTATGGCTAATTATGAAGTTGTATTATTATTATTTATATAAAGGTGAATATGTACATGGGATGGGGAAACCATTTTTTAGTATATATACATCTATATATATTAGTCATCTTATAGGAAGGCTCAACCAAAGGTTTTGTAACCTTCCACTGTGTGTACAAGCAGTTTGTGGCTATTTAGTTTCATATGATAGATAAGTTTGTCCTTTCTGGCAATGATATTAATGATAGTGTTTTTTTTTAARTKTTA
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00537 Ps_contig_mira-and-tgicl-ass_franssen_5262
SEQUENCE=AAACTAAAAAAAACACTATCATTAATATCATTGCCAGAAAGGACAAACTTATCTATCATATGAAACTAAATAGCCACAAACTGCTTGTACACACAGTGGAAGGTTACAAACCTTTGGTTGAGCCTTCCTATAAGATGACTAATATATATAGATGTATATATAACTAAAAATGTTCCCCACTCCCATGTACATATTCACCTTATATAAATAATAATAATACACTTCATAATTAG
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00538 Ps_contig_mira-and-tgicl-ass_franssen_5263
SEQUENCE=CAGTGGTATCAACGCAGAGACGCGGGGAACAAGTAAAAAGATTAGAAATTTTCATGCAAAAACCACTAGACTTTCTTTTTATTTATTATTATTATTATGAGGTG
TARGET=83,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00539 Ps_contig_mira-and-tgicl-ass_franssen_5264
SEQUENCE=ATATAATAATCAAATTAATCACTAAAGATTTATAAAATCACACAGCTTTAACTAGAAGGATCTATATAACTTCAGCCATATTATTTCACTGTTTTCAAAGAATTCTTGTTATGAACTTGAGAATCCATTCTTGTGTCTTGAGATTGTTTTGGATATTTTGAGTATGAATTAGATCTTCCTTTCTTGTGCTTCCTTCTTGGTTGTGTTGCTTGTGGAATAAACACTCCTGTTCCTTTGCTTTCTCTTCTCACCAAAGCTTGCTCATAGAGGATATCAAACTGTGGAGGAGGATGAATTGCTCTAGAAAAAGCCTGAAAAATGAAACTTAACAATTATGTCGGTGCTATAGAATATTATATGCAAAAGGAAAAAAAATGTAGTTATGAGGTGTGTGAAGTAATTAAGGAGGTGTAATTAGTAAAATACCTGAAAAGAGTTTTGAGGTTGAGGAACAAAAGGATCTTCATCTTCATCCATAATTAAGAGAGCAATTTCCTTGCTCAAATTTGACAAAAAAAAAATCATCATCTAATTCAATAGCAACCTCCATAATAATAATAATAATAAAAAGAAAGTCTAGTGGTTTTGCATGAAAATTTCTAATCTTTTACTTGTTGTTCTCCMTAACTGGGGGATGTTTGGTTTTATATAGAGAAAAAGAACGGTAAGGA
TARGET=549,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00540 Ps_contig_mira-and-tgicl-ass_franssen_5274
SEQUENCE=AGCTCAAGGAATAAGTTGCTAGAGTCATCGCCCTGATGGAGTCACTCATTGCTGCTCATAACCAACCAGCTCCAACCCCTGCAACTCCTCAGCAAGGACTGTCATCTCTGAGATTATTGCAACACTAGTGTTTGTGTTTCCTGCCAGCCAGCCTGCTCAAGCTATGCATGCCAGATTCCCATGGGAAATGCCTCCTAATTTTATGCCTGAAGGTTATGCTCCAAATGTTGCTCCTATGCCGACATCTCACGGTCATGTCAATACCTCATCCTGTGGTGTATGTTATGCCACATGTTGATGAAACCATTTATCATTCTGAGCCGTATGAGGGCCTTGATGTGTATGAGAAAATGGATGAGATGAAAGACCAATTTCAAGAGTTAAGAAAATAATTGAAGACCTTGAGAGGTAAAAATTTGTTTGCAAAAAGTGCTGCTAAACTTTGCTTGGTGCCAAACGTGAAAATCCCTATCAAATTCAAGGTCCCAGATTTTGAGAAGTATAAAGGGAATACTTGTCCATTGAGCCATCTTGTGATGTATGCGCGCAAAATATCGACCCAGATAGATAGTGTTCAATTGCTCATCCATTATTTTCAAGACAATTTGACTGCTGTCATGCTTCAGCGGTATATGGGTTTAGACAGTGCTTGTGTCCATACTTTCAACGACTTAGGCGAGGCCTCCGTCAAGCAATACAAGTACAACATATATATAATGCCAGATCGTGATCAGTTGAGGTCCATGTCTCAGAAGGATAATGAGACATTCAAAGAGTATGCCCAGAGGTGGAGAGAGCTTGCCGCCTAGATTAGTCCCCCATTGGAGGAAAAGTAGATGACGAAGATCTTTTTGAAGACTTTGAGTTTGTTCTATTACGAGCGTATGATTTCCAGTGCTCCCAGTGACTTCACTAAAATGGTGAACATGGGGATTTGTTTAGAAGAGGGTGTCCGAGAGGGCCGTTTGTCTAAAGAAGAAGCATCTACGAGAAATAAGTATGGATGCAATTTCTCTAAGAAGAAGGAAGGGGAAACCAATTCAGTATCTGTGGGGAGGCAAAAGAGGCCTCATGTGAGGAGAAATTCCAGATCTCAGTATCATCATCATCATCAAGTATCCTATGCCATTCCAGCATTCACCAATAATTTCAATCATCTCCAGTTCAACATCAACAACAATGTACTCACAACAATCACAACAATTACAACAATCATCATAACAATTTTGAAAGGAAGAAAGTCACTTTTGATCCTATTCTGAGTCGTATGCAGAATTGTATCCTTCTCTCATCGTCAAGAATTTGATCCAGCTAAGGAATCCTCCGCATACACATGAGCCTCTGCCATGGTGGTCAAGCCCGATCTGCATTATGCTTTATATCAAGGGGCACCCGACCATGACATTGAGAATTGTTATCCTTTGAAGTATGAAGTTAAAAAATTGGTCAAAAGTGGGATGGTGTCCTTTGAGGACCGCGCACCTAATGTCAAAGCTAATCCTTTGCCCTCCCATGCAATTCTTTTATGAATATGGTAGATGGTTGTCTTGGTAATTTCAGAGTTTTTGATGTTCGTCGTATCTGAAGGTCTTTGGTGGAAATACATAGAACTTTGTGCATGATCAGTGATTGTGAACATGACCATGACAATTGTGTTATATACAATGTTAATCCCCGAGAATGTGCGATTGTGAAGAGGGACGTTTAGAAGTTGATGGGTGAAGGTGTTATTCAGATTAATCAGTCCAGAAATTTGGGTAATGATGTGAATGTAATTGTGCCAGTATTCAAGACTCCCGAGCGAGTAGTAATCCAGTTTGATAGCAGCAAAAGAGATAATAGATCGATATCGCTGTTGGTAATACGGTTAGCGGGCCCTATCCCTTATGCATCCGATAAAGTTGTACCTTACAAATATAATGCTACTATTATTGAGAATGGGCAAGAGGTTCCTCTTCCCGCAACAAATTCAGTAGTGAGCATTGCTGATACTGTGAAAGTGACCCATATCGGTCATGTATTTGGTTTAGTACCCATGAAGGTTATGGAGAATGTTGTGGTTGGTAAGAAGGCGAATGTTCCAGTGGTTAATCCTATTAACCCTCCAACTTTCCAGTCTGGTGAATCCAGCGGGTTGAAGGTTAAGGATGATGATGATGAGATTCTCTAGTTGATTAAGAAGAGTGAGTTCAATGTTATGGAGTAGATGCTCCAAACACCATCCAAGATCTTTATGATTTTATTGATGAATTATGAAGCGCACCGAGAGGCGTTGCAGAAGGTGCTTGAGAAAACCTATGTGGAACAGGAATTTACGGTTGATCAGTTTGAACATATCGTTGCCAATATTACTTCATGCAATAATTTGAGCTTTTGTGATCAATATATTAAAAAAAAAATTAAAAAATCAATCTAAAGGAACAAATCAATCAAATTTTATATCTACACCTTTAAATTAATCAATTTTAAAATACTCCTCTGCTTTCCATAATATGTCACTTTCATACATATTAAAAAATTATTTATTAACATGAAAAGGAAAAATGATGCATTATTTTATTAAATAATTGTATTAATAGTATAGGGAAAATAAAGTAATGAGCCGAAAGAAAAGATAGTAACTAATATTTAATAAAAGAGGTTCTAGTAGTACATRRSKKAAGAATTGTATTAAAAAATGAAAACCACATGCTCTTATTCTTTGGATTTGTAATCCTTATCTTCAAAATGGAATATTATTTTGTCTATATATTCAGTTTGATCAAGTATACAAAGATCAKATAGTGAGCAAACCAGTGCTAGCTAACTTATCAGTATGGCTATCCAAGAAACTTTGATAGACAAGCCTAGAAAATCCATTCCCAAAACTTTCTGGTTAATCCTCTCTTTAGCTGCTATCATAGGCTCATCAGCCCTTATTGTTTCTCATCTCAACAAACCTATCTCCTTCTTCCCACTCTCTTCAGCTCCCAATCTGTGTGAGCATGCTGTTGATACAAAATCATGCTTAACTCATGTATCAGAAGTGGTTCAAGGCCAAGCCTTAGCTAACACAAAAGACCACAAATTGAGTACACTCATATCCTTATTAACCAAGTCCACCTCACACATTCAGAAAGCCATGGAAACAGCCAATGTTATCAAACGCCGGGTTAACAGCCCTAGAGAGGAGACGGCTTTGAATGACTGTGAGCAACTAATGGACTTGTCCATGGATAGAGTTTGGGACTCAGTGTTGACTTTAACAAAAAATAACATTGACTCACAACAAGATGCACACACATGGCTAAGTAGTGTGCTCACTAACCATGCAACTTGTTTGAATGGTTTAGAAGGTACATCTCGGGTCGTCATGGAAAGTGACCTTCAGGACTTGATATCAAGAGCTAGATCTTCTCTCGCCGTGCTTGTTTCCGTTTTACCTGCAAAGAGTAACGACGGATTCATTGATGAATCATTGAACGGTGAATTTCCCTCATGGGTAACGAGTAAGGATCGAAGGCTTTTGGAGTCTACAGTTGGGGACATAAAGGCCAATGTTGTGGTGGCTAAAGATGGGAGTGGTAAGTTCAAAACTGTGGCTGAGGCTGTGGCATCTGCACCAGACAACGGTAAGGCAAGGTATGTTATCTATGTGAAAAGAGGAACCTACAAAGAGAAGGTAGAAATTGGTAAGAAAAAGACAAATGTGATGCTCGTTGGTGATGGTATGGATGCAACAATAATCACAGGCAACTTGAATTTTATCGATGGAACAACCACTTTCAATAGTGCAACTGTTGCTGCTGTTGGAGATGGGTTTATAGCTCAGGACATAGGGTTCCAAAACACGGCAGGCCCAGAAAAGCACCAGGCAGTTGCTCTCCGCGTAGGTGCTGATCAATCTGTCATCAACCGTTGTAAAATTGACGCATTTCAAGACACCCTCTACGCACACTCCAACCGACAATTTTACCGTGACTCCTTCATTACCGGTACTGTTGACTTTATCTTTGGAAACGCAGGTGTTGTGTTCCAGAAGAGCAAACTTGTGGCCCGAAAGCCCATGAGCAACCAAAAGAACATGGTCACGGCCCAAGGTCGAGAAGACCCAAACCAGAACACTGCAACTTCAATTCAGCAATGTAATGTCATACCAAGCTCGGACCTCAAGCCTGTGCAAGGCTCCATCAAAACATACCTAGGCCGCCCATGGAAGAAATACTCCAGGACTGTTGTGTTGCAGTCCGTCGTAGACAGCCATATTGACCCAGCAGGATGGGCTGAATGGGATGCCGCGAGTAAGGATTTTCTGCAAACATTGTATTACGGAGAGTACTTGAACAGTGGAGCAGGTGCTGGTACCAGCAAGAGAGTGACTTGGCCTGGTTATCATATCATCAAAACTGCTGCAGAGGCTAGCAAGTTTACAGTGACACAGCTCATCCAGGGTAATGTTTGGTTGAAGAACACAGGGGTAGCCTTCATTGAAGGCCTGTAGAAATTGGCTTCGGCAGGCGTGTACTATTATGTTTTTGATATGAGTGAAATTTGCAGGAAATAAAACAGAAACATTATCCTTTATGGAAGAATGAGGTTTAATTAAAAAAATTTCCTACATACGATGTAACAACTGTCTTCTACCAATGTTAAAATGAATGCCACATGTCTGGAAAATAAACTTTAGTAGTTAACARCC
TARGET=1099,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00541 Ps_contig_mira-and-tgicl-ass_franssen_5274
SEQUENCE=AGCTCAAGGAATAAGTTGCTAGAGTCATCGCCCTGATGGAGTCACTCATTGCTGCTCATAACCAACCAGCTCCAACCCCTGCAACTCCTCAGCAAGGACTGTCATCTCTGAGATTATTGCAACACTAGTGTTTGTGTTTCCTGCCAGCCAGCCTGCTCAAGCTATGCATGCCAGATTCCCATGGGAAATGCCTCCTAATTTTATGCCTGAAGGTTATGCTCCAAATGTTGCTCCTATGCCGACATCTCACGGTCATGTCAATACCTCATCCTGTGGTGTATGTTATGCCACATGTTGATGAAACCATTTATCATTCTGAGCCGTATGAGGGCCTTGATGTGTATGAGAAAATGGATGAGATGAAAGACCAATTTCAAGAGTTAAGAAAATAATTGAAGACCTTGAGAGGTAAAAATTTGTTTGCAAAAAGTGCTGCTAAACTTTGCTTGGTGCCAAACGTGAAAATCCCTATCAAATTCAAGGTCCCAGATTTTGAGAAGTATAAAGGGAATACTTGTCCATTGAGCCATCTTGTGATGTATGCGCGCAAAATATCGACCCAGATAGATAGTGTTCAATTGCTCATCCATTATTTTCAAGACAATTTGACTGCTGTCATGCTTCAGCGGTATATGGGTTTAGACAGTGCTTGTGTCCATACTTTCAACGACTTAGGCGAGGCCTCCGTCAAGCAATACAAGTACAACATATATATAATGCCAGATCGTGATCAGTTGAGGTCCATGTCTCAGAAGGATAATGAGACATTCAAAGAGTATGCCCAGAGGTGGAGAGAGCTTGCCGCCTAGATTAGTCCCCCATTGGAGGAAAAGTAGATGACGAAGATCTTTTTGAAGACTTTGAGTTTGTTCTATTACGAGCGTATGATTTCCAGTGCTCCCAGTGACTTCACTAAAATGGTGAACATGGGGATTTGTTTAGAAGAGGGTGTCCGAGAGGGCCGTTTGTCTAAAGAAGAAGCATCTACGAGAAATAAGTATGGATGCAATTTCTCTAAGAAGAAGGAAGGGGAAACCAATTCAGTATCTGTGGGGAGGCAAAAGAGGCCTCATGTGAGGAGAAATTCCAGATCTCAGTATCATCATCATCATCAAGTATCCTATGCCATTCCAGCATTCACCAATAATTTCAATCATCTCCAGTTCAACATCAACAACAATGTACTCACAACAATCACAACAATTACAACAATCATCATAACAATTTTGAAAGGAAGAAAGTCACTTTTGATCCTATTCTGAGTCGTATGCAGAATTGTATCCTTCTCTCATCGTCAAGAATTTGATCCAGCTAAGGAATCCTCCGCATACACATGAGCCTCTGCCATGGTGGTCAAGCCCGATCTGCATTATGCTTTATATCAAGGGGCACCCGACCATGACATTGAGAATTGTTATCCTTTGAAGTATGAAGTTAAAAAATTGGTCAAAAGTGGGATGGTGTCCTTTGAGGACCGCGCACCTAATGTCAAAGCTAATCCTTTGCCCTCCCATGCAATTCTTTTATGAATATGGTAGATGGTTGTCTTGGTAATTTCAGAGTTTTTGATGTTCGTCGTATCTGAAGGTCTTTGGTGGAAATACATAGAACTTTGTGCATGATCAGTGATTGTGAACATGACCATGACAATTGTGTTATATACAATGTTAATCCCCGAGAATGTGCGATTGTGAAGAGGGACGTTTAGAAGTTGATGGGTGAAGGTGTTATTCAGATTAATCAGTCCAGAAATTTGGGTAATGATGTGAATGTAATTGTGCCAGTATTCAAGACTCCCGAGCGAGTAGTAATCCAGTTTGATAGCAGCAAAAGAGATAATAGATCGATATCGCTGTTGGTAATACGGTTAGCGGGCCCTATCCCTTATGCATCCGATAAAGTTGTACCTTACAAATATAATGCTACTATTATTGAGAATGGGCAAGAGGTTCCTCTTCCCGCAACAAATTCAGTAGTGAGCATTGCTGATACTGTGAAAGTGACCCATATCGGTCATGTATTTGGTTTAGTACCCATGAAGGTTATGGAGAATGTTGTGGTTGGTAAGAAGGCGAATGTTCCAGTGGTTAATCCTATTAACCCTCCAACTTTCCAGTCTGGTGAATCCAGCGGGTTGAAGGTTAAGGATGATGATGATGAGATTCTCTAGTTGATTAAGAAGAGTGAGTTCAATGTTATGGAGTAGATGCTCCAAACACCATCCAAGATCTTTATGATTTTATTGATGAATTATGAAGCGCACCGAGAGGCGTTGCAGAAGGTGCTTGAGAAAACCTATGTGGAACAGGAATTTACGGTTGATCAGTTTGAACATATCGTTGCCAATATTACTTCATGCAATAATTTGAGCTTTTGTGATCAATATATTAAAAAAAAAATTAAAAAATCAATCTAAAGGAACAAATCAATCAAATTTTATATCTACACCTTTAAATTAATCAATTTTAAAATACTCCTCTGCTTTCCATAATATGTCACTTTCATACATATTAAAAAATTATTTATTAACATGAAAAGGAAAAATGATGCATTATTTTATTAAATAATTGTATTAATAGTATAGGGAAAATAAAGTAATGAGCCGAAAGAAAAGATAGTAACTAATATTTAATAAAAGAGGTTCTAGTAGTACATRRSKKAAGAATTGTATTAAAAAATGAAAACCACATGCTCTTATTCTTTGGATTTGTAATCCTTATCTTCAAAATGGAATATTATTTTGTCTATATATTCAGTTTGATCAAGTATACAAAGATCAKATAGTGAGCAAACCAGTGCTAGCTAACTTATCAGTATGGCTATCCAAGAAACTTTGATAGACAAGCCTAGAAAATCCATTCCCAAAACTTTCTGGTTAATCCTCTCTTTAGCTGCTATCATAGGCTCATCAGCCCTTATTGTTTCTCATCTCAACAAACCTATCTCCTTCTTCCCACTCTCTTCAGCTCCCAATCTGTGTGAGCATGCTGTTGATACAAAATCATGCTTAACTCATGTATCAGAAGTGGTTCAAGGCCAAGCCTTAGCTAACACAAAAGACCACAAATTGAGTACACTCATATCCTTATTAACCAAGTCCACCTCACACATTCAGAAAGCCATGGAAACAGCCAATGTTATCAAACGCCGGGTTAACAGCCCTAGAGAGGAGACGGCTTTGAATGACTGTGAGCAACTAATGGACTTGTCCATGGATAGAGTTTGGGACTCAGTGTTGACTTTAACAAAAAATAACATTGACTCACAACAAGATGCACACACATGGCTAAGTAGTGTGCTCACTAACCATGCAACTTGTTTGAATGGTTTAGAAGGTACATCTCGGGTCGTCATGGAAAGTGACCTTCAGGACTTGATATCAAGAGCTAGATCTTCTCTCGCCGTGCTTGTTTCCGTTTTACCTGCAAAGAGTAACGACGGATTCATTGATGAATCATTGAACGGTGAATTTCCCTCATGGGTAACGAGTAAGGATCGAAGGCTTTTGGAGTCTACAGTTGGGGACATAAAGGCCAATGTTGTGGTGGCTAAAGATGGGAGTGGTAAGTTCAAAACTGTGGCTGAGGCTGTGGCATCTGCACCAGACAACGGTAAGGCAAGGTATGTTATCTATGTGAAAAGAGGAACCTACAAAGAGAAGGTAGAAATTGGTAAGAAAAAGACAAATGTGATGCTCGTTGGTGATGGTATGGATGCAACAATAATCACAGGCAACTTGAATTTTATCGATGGAACAACCACTTTCAATAGTGCAACTGTTGCTGCTGTTGGAGATGGGTTTATAGCTCAGGACATAGGGTTCCAAAACACGGCAGGCCCAGAAAAGCACCAGGCAGTTGCTCTCCGCGTAGGTGCTGATCAATCTGTCATCAACCGTTGTAAAATTGACGCATTTCAAGACACCCTCTACGCACACTCCAACCGACAATTTTACCGTGACTCCTTCATTACCGGTACTGTTGACTTTATCTTTGGAAACGCAGGTGTTGTGTTCCAGAAGAGCAAACTTGTGGCCCGAAAGCCCATGAGCAACCAAAAGAACATGGTCACGGCCCAAGGTCGAGAAGACCCAAACCAGAACACTGCAACTTCAATTCAGCAATGTAATGTCATACCAAGCTCGGACCTCAAGCCTGTGCAAGGCTCCATCAAAACATACCTAGGCCGCCCATGGAAGAAATACTCCAGGACTGTTGTGTTGCAGTCCGTCGTAGACAGCCATATTGACCCAGCAGGATGGGCTGAATGGGATGCCGCGAGTAAGGATTTTCTGCAAACATTGTATTACGGAGAGTACTTGAACAGTGGAGCAGGTGCTGGTACCAGCAAGAGAGTGACTTGGCCTGGTTATCATATCATCAAAACTGCTGCAGAGGCTAGCAAGTTTACAGTGACACAGCTCATCCAGGGTAATGTTTGGTTGAAGAACACAGGGGTAGCCTTCATTGAAGGCCTGTAGAAATTGGCTTCGGCAGGCGTGTACTATTATGTTTTTGATATGAGTGAAATTTGCAGGAAATAAAACAGAAACATTATCCTTTATGGAAGAATGAGGTTTAATTAAAAAAATTTCCTACATACGATGTAACAACTGTCTTCTACCAATGTTAAAATGAATGCCACATGTCTGGAAAATAAACTTTAGTAGTTAACARCC
TARGET=2147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00542 Ps_contig_mira-and-tgicl-ass_franssen_5275
SEQUENCE=CTCAAAATACACAACACCACAAGCATACGAAATTGATATATTTACCGAAGCTGTGTCACTGTTGTCGTTTGTTGTTGCTGCGAAGCTGGAAGGTTGGTTGCTTGGTTTCGCCCGTTGGGGAAGAGAGAATCTGCGTCGCGGTTGGTCGTGGTGAATTTAGAAGAGCGAACCGCGGCGGCGGCGATGTTGGTGAACAGAGGTGGATTCGCCTGAGTGTGAAGGAGCTTGGTTATTGACGCGACGGTGAGGTTTGGAATTTGTCGCCGGAATAGATGAGGAAGACGGCAGATGGTTCAAAATTGACGGTGGTTGAGCGATGATGCGGTGACGTAGTGGTAGTGGTGGCGATTCGACGGAGGTTAGTCTAGGTTTGTTTTGGCGGTTTAGGTAAAAGGGGGTTTTGTTGGTTTGTTGKTWAAAGGGTGTGTCGGTGAGGTTGTTGCGAGACGGAGGAAGACGATGGGTGAGTGGAAGCTATGGCCTCATAGTGGTAGTGGTGGCGATTCACGGTGGAGGATTTTTG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00543 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2474
PRIMER_LEFT_PENALTY=0.089133
PRIMER_RIGHT_PENALTY=0.158297
PRIMER_LEFT_SEQUENCE=AGTATGCATCGTTGAGAAGCAA
PRIMER_RIGHT_SEQUENCE=ACTCGATGCGGTCTTTGTAGAT
PRIMER_LEFT=170,22
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=59.911
PRIMER_RIGHT_TM=60.158
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.3542
PRIMER_LEFT_1_PENALTY=0.089133
PRIMER_RIGHT_1_PENALTY=0.265053
PRIMER_LEFT_1_SEQUENCE=AGTATGCATCGTTGAGAAGCAA
PRIMER_RIGHT_1_SEQUENCE=GGTCTTTGTAGATTGGGTCCAC
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=391,22
PRIMER_LEFT_1_TM=59.911
PRIMER_RIGHT_1_TM=59.735
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.3843
PRIMER_LEFT_2_PENALTY=0.119274
PRIMER_RIGHT_2_PENALTY=0.265053
PRIMER_LEFT_2_SEQUENCE=CATGTATCCGTTATTGCAGCAT
PRIMER_RIGHT_2_SEQUENCE=GGTCTTTGTAGATTGGGTCCAC
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=391,22
PRIMER_LEFT_2_TM=59.881
PRIMER_RIGHT_2_TM=59.735
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.4495
PRIMER_LEFT_3_PENALTY=0.089133
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=AGTATGCATCGTTGAGAAGCAA
PRIMER_RIGHT_3_SEQUENCE=TCAGATAGCTTGATGGAAGGCT
PRIMER_LEFT_3=170,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=59.911
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.4796
PRIMER_LEFT_4_PENALTY=0.119274
PRIMER_RIGHT_4_PENALTY=0.360320
PRIMER_LEFT_4_SEQUENCE=CATGTATCCGTTATTGCAGCAT
PRIMER_RIGHT_4_SEQUENCE=TCAGATAGCTTGATGGAAGGCT
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=59.881
PRIMER_RIGHT_4_TM=60.360
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_00544 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=618,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1733
PRIMER_LEFT_PENALTY=0.054514
PRIMER_RIGHT_PENALTY=0.118777
PRIMER_LEFT_SEQUENCE=GGTTTGGTTGGGATTATCTTGA
PRIMER_RIGHT_SEQUENCE=TTCTTCATCATGTGTCGTCCTC
PRIMER_LEFT=479,22
PRIMER_RIGHT=726,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=60.119
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1733
PRIMER_LEFT_1_PENALTY=0.054514
PRIMER_RIGHT_1_PENALTY=0.118777
PRIMER_LEFT_1_SEQUENCE=GGTTTGGTTGGGATTATCTTGA
PRIMER_RIGHT_1_SEQUENCE=TCTTCTTCATCATGTGTCGTCC
PRIMER_LEFT_1=479,22
PRIMER_RIGHT_1=728,22
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=60.119
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.1796
PRIMER_LEFT_2_PENALTY=0.060824
PRIMER_RIGHT_2_PENALTY=0.118777
PRIMER_LEFT_2_SEQUENCE=ACAGGACATAAGCGACAGGTTT
PRIMER_RIGHT_2_SEQUENCE=TTCTTCATCATGTGTCGTCCTC
PRIMER_LEFT_2=462,22
PRIMER_RIGHT_2=726,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=60.119
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.1796
PRIMER_LEFT_3_PENALTY=0.060824
PRIMER_RIGHT_3_PENALTY=0.118777
PRIMER_LEFT_3_SEQUENCE=ACAGGACATAAGCGACAGGTTT
PRIMER_RIGHT_3_SEQUENCE=TCTTCTTCATCATGTGTCGTCC
PRIMER_LEFT_3=462,22
PRIMER_RIGHT_3=728,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=60.119
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.2056
PRIMER_LEFT_4_PENALTY=0.054514
PRIMER_RIGHT_4_PENALTY=0.151106
PRIMER_LEFT_4_SEQUENCE=GGTTTGGTTGGGATTATCTTGA
PRIMER_RIGHT_4_SEQUENCE=GTAGTAGCAGGTCGGAGATTGG
PRIMER_LEFT_4=479,22
PRIMER_RIGHT_4=662,22
PRIMER_LEFT_4_TM=60.055
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_00545 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=722,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2185
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.067430
PRIMER_LEFT_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT=641,22
PRIMER_RIGHT=786,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.2185
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.067430
PRIMER_LEFT_1_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_1_SEQUENCE=TAATATCAGCAGCAGGCACAGT
PRIMER_LEFT_1=641,22
PRIMER_RIGHT_1=787,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.2557
PRIMER_LEFT_2_PENALTY=0.188254
PRIMER_RIGHT_2_PENALTY=0.067430
PRIMER_LEFT_2_SEQUENCE=CCAGTCTCCCACCTGCTACTAC
PRIMER_RIGHT_2_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_2=602,22
PRIMER_RIGHT_2=786,22
PRIMER_LEFT_2_TM=60.188
PRIMER_RIGHT_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=59.091
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.2837
PRIMER_LEFT_3_PENALTY=0.188254
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=CCAGTCTCCCACCTGCTACTAC
PRIMER_RIGHT_3_SEQUENCE=GATAGTTGATGGCTTTTCGGAG
PRIMER_LEFT_3=602,22
PRIMER_RIGHT_3=847,22
PRIMER_LEFT_3_TM=60.188
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=59.091
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.1000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.2995
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.067430
PRIMER_LEFT_4_SEQUENCE=GCAAAGTTGATTTCGACCTTCT
PRIMER_RIGHT_4_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_4=515,22
PRIMER_RIGHT_4=786,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_00546 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=739,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2142
PRIMER_LEFT_PENALTY=0.118777
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GAGGACGACACATGATGAAGAA
PRIMER_RIGHT_SEQUENCE=GATAGTTGATGGCTTTTCGGAG
PRIMER_LEFT=705,22
PRIMER_RIGHT=847,22
PRIMER_LEFT_TM=60.119
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.2142
PRIMER_LEFT_1_PENALTY=0.118777
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=GGACGACACATGATGAAGAAGA
PRIMER_RIGHT_1_SEQUENCE=GATAGTTGATGGCTTTTCGGAG
PRIMER_LEFT_1=707,22
PRIMER_RIGHT_1=847,22
PRIMER_LEFT_1_TM=60.119
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.2185
PRIMER_LEFT_2_PENALTY=0.151106
PRIMER_RIGHT_2_PENALTY=0.067430
PRIMER_LEFT_2_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_2_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_2=641,22
PRIMER_RIGHT_2=786,22
PRIMER_LEFT_2_TM=60.151
PRIMER_RIGHT_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.2185
PRIMER_LEFT_3_PENALTY=0.151106
PRIMER_RIGHT_3_PENALTY=0.067430
PRIMER_LEFT_3_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_3_SEQUENCE=TAATATCAGCAGCAGGCACAGT
PRIMER_LEFT_3=641,22
PRIMER_RIGHT_3=787,22
PRIMER_LEFT_3_TM=60.151
PRIMER_RIGHT_3_TM=59.933
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.1000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.2557
PRIMER_LEFT_4_PENALTY=0.188254
PRIMER_RIGHT_4_PENALTY=0.067430
PRIMER_LEFT_4_SEQUENCE=CCAGTCTCCCACCTGCTACTAC
PRIMER_RIGHT_4_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_4=602,22
PRIMER_RIGHT_4=786,22
PRIMER_LEFT_4_TM=60.188
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_00547 Ps_contig_mira-and-tgicl-ass_franssen_5282
SEQUENCE=AGAAGCACCCTATCATTTKGTATCGTTATCTTCTCAAGTATTGATATAACCCTAGCTCCGAACACCGCTTTCTTCTTTTTCTAATCATCTCATTTTCTTCTCTCTCCAAACCCTAATACCAATTTTCCATTTTCACTCCAACTTCGTTGTATCAATTTCTCTCCATTCATTTCCTTCTAGATCTCAAACTCTTCCTTTTCTTTTTCTCGATTAAATCAAATAATACAAAAGCTTCGTTCTTTCTATAACCGCTTCACCTTTTTGAATTTCAGCGATATATAAAACCCGATTCAGTCCCAATTTTGATTTCAACCGACAACAACACAAGCGGCGAGGTTTGGAATTTCCGAGAGTAGTGAAAATCGCTGTTGACAATGGTTGGCGGTGGAAGCAGAAGAGATGATGGGTCGTTGGTAATTAACAACACCAACGTGTTTGCGGCGCTTGATACTTTGAAGAAGAAAAAGAAATCGGATAAGGAGAGAAAGAATAAGGGTTCCAAATCCACCAAATTGGAATCTGAATCGGATTCAAAGGTGTTTTGGGCTCCGGCCCCATTGAATGCATCTTCATGGGCTGATGTGGATGATGATGATGATTATTATGCCACCACTGCACCGCCTCAATCTGTTTGGTCTGTTTCCGACCCCCAAAACAGTGAGGACAAGCCAGAGATTTTTGAGGATACTGAGAGTGAGGAAGATATTTTAGATGATGGGGATGAGGAGGAGGAAGAGCAAGATCATGAACCAGAACCAGAGGACGCTGTGAAACCTGAACCTGAGCTCAAGAAGCATGCTGAAGTTCCTGTGGCACCAAAGGAAGCTGAACGGCAGCTCTCCAAAAAGGAAAGGAAGAAAAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTCGCACAGAAAGAAAGTAACGATGGTCAAGGTCAAGATGAGTCGCAAGGCGCACCTGAGGATAAGAAAGATGTTGAAGGGGATGTAAATGGAGAAAAGAAAGAAATAGCTGTGGAGTCAAAGAATGCCAAGAAGAAGAAAAAGAAGGAAAAAGCTTCCAAGGAGGTGAAAGAATCCAACGGTCAGCACAGCAATTCAGAAACAAACGTTGTGTCTGATATGGCCGAAAATGCGGAGGAGGATCCATCTGTTGTTGATGTGAAAGAGCGTCTTAAGAAACTTACTTCTATCAAGAAGAAGAAATCCAATAAAGAAATGGATGGTGCTGCTAGAGCTGCTGCTCAAGAAGCTGCTGCAAGGAATGCAAGGCTTGCTGCAGCAAAGAAAAAAGAGAAGAACCATTATAACCAACAACCTGTGCGGTAAAAGAATCTTGCTCCTAAACATCTATCTATACAGGAATTTGTATTTCTTGTCTATATGATGATGATGATGATGATGATGCCGAATAAATAGTCGGGCTTTAGGCTCTGGTTACCCTAGCCGTACTCCAGTAGACTTGATATACTGTCTTATGCTTTTTTGGGCTAGTGTTGTTTAATTGTTATAGACCATTATATTTTGAGCACGGGATTTTATCTCTAAAAATTACAAGAACTATGTTTTGAAAATCCCATGGGACTAAGTTGTTGTGCATTTGTTCTTATGTCGTGGCTACTGCTGGTGCGAGTAGTTGAAGTTTAGTTTTGAAAGTGTTGTGTTGTGTACTACAGACAGATTAAAAAATATTCTTCAAGAAAA
TARGET=585,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00548 Ps_contig_mira-and-tgicl-ass_franssen_5282
SEQUENCE=AGAAGCACCCTATCATTTKGTATCGTTATCTTCTCAAGTATTGATATAACCCTAGCTCCGAACACCGCTTTCTTCTTTTTCTAATCATCTCATTTTCTTCTCTCTCCAAACCCTAATACCAATTTTCCATTTTCACTCCAACTTCGTTGTATCAATTTCTCTCCATTCATTTCCTTCTAGATCTCAAACTCTTCCTTTTCTTTTTCTCGATTAAATCAAATAATACAAAAGCTTCGTTCTTTCTATAACCGCTTCACCTTTTTGAATTTCAGCGATATATAAAACCCGATTCAGTCCCAATTTTGATTTCAACCGACAACAACACAAGCGGCGAGGTTTGGAATTTCCGAGAGTAGTGAAAATCGCTGTTGACAATGGTTGGCGGTGGAAGCAGAAGAGATGATGGGTCGTTGGTAATTAACAACACCAACGTGTTTGCGGCGCTTGATACTTTGAAGAAGAAAAAGAAATCGGATAAGGAGAGAAAGAATAAGGGTTCCAAATCCACCAAATTGGAATCTGAATCGGATTCAAAGGTGTTTTGGGCTCCGGCCCCATTGAATGCATCTTCATGGGCTGATGTGGATGATGATGATGATTATTATGCCACCACTGCACCGCCTCAATCTGTTTGGTCTGTTTCCGACCCCCAAAACAGTGAGGACAAGCCAGAGATTTTTGAGGATACTGAGAGTGAGGAAGATATTTTAGATGATGGGGATGAGGAGGAGGAAGAGCAAGATCATGAACCAGAACCAGAGGACGCTGTGAAACCTGAACCTGAGCTCAAGAAGCATGCTGAAGTTCCTGTGGCACCAAAGGAAGCTGAACGGCAGCTCTCCAAAAAGGAAAGGAAGAAAAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTCGCACAGAAAGAAAGTAACGATGGTCAAGGTCAAGATGAGTCGCAAGGCGCACCTGAGGATAAGAAAGATGTTGAAGGGGATGTAAATGGAGAAAAGAAAGAAATAGCTGTGGAGTCAAAGAATGCCAAGAAGAAGAAAAAGAAGGAAAAAGCTTCCAAGGAGGTGAAAGAATCCAACGGTCAGCACAGCAATTCAGAAACAAACGTTGTGTCTGATATGGCCGAAAATGCGGAGGAGGATCCATCTGTTGTTGATGTGAAAGAGCGTCTTAAGAAACTTACTTCTATCAAGAAGAAGAAATCCAATAAAGAAATGGATGGTGCTGCTAGAGCTGCTGCTCAAGAAGCTGCTGCAAGGAATGCAAGGCTTGCTGCAGCAAAGAAAAAAGAGAAGAACCATTATAACCAACAACCTGTGCGGTAAAAGAATCTTGCTCCTAAACATCTATCTATACAGGAATTTGTATTTCTTGTCTATATGATGATGATGATGATGATGATGCCGAATAAATAGTCGGGCTTTAGGCTCTGGTTACCCTAGCCGTACTCCAGTAGACTTGATATACTGTCTTATGCTTTTTTGGGCTAGTGTTGTTTAATTGTTATAGACCATTATATTTTGAGCACGGGATTTTATCTCTAAAAATTACAAGAACTATGTTTTGAAAATCCCATGGGACTAAGTTGTTGTGCATTTGTTCTTATGTCGTGGCTACTGCTGGTGCGAGTAGTTGAAGTTTAGTTTTGAAAGTGTTGTGTTGTGTACTACAGACAGATTAAAAAATATTCTTCAAGAAAA
TARGET=1383,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00549 Ps_contig_mira-and-tgicl-ass_franssen_5300
SEQUENCE=TCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACCGCTGCAGCAAAGAAGCCACTTTCTCATCCCACTTTCGCTGAGGTAGAAATCAAAATCCCGTTCAATTTTCAGTATTTTTGAATATTTTTTTTGGGTGTGTATATTGATTTTGTTTTTTCTTATTTCCTTTTGAAGATGATAACTGAAGCAATCACAAGCCTGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAATTCATCGAAGAGAAATACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAAGGTGAAAAGCTCCTTCAAACTCGCTCCGGCAGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAGCTGTCACAAAGCCCAAGGCTAAAGCTGCTGGGAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTATTATTATTATTGG
TARGET=674,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00550 Ps_contig_mira-and-tgicl-ass_franssen_5300
SEQUENCE=TCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACCGCTGCAGCAAAGAAGCCACTTTCTCATCCCACTTTCGCTGAGGTAGAAATCAAAATCCCGTTCAATTTTCAGTATTTTTGAATATTTTTTTTGGGTGTGTATATTGATTTTGTTTTTTCTTATTTCCTTTTGAAGATGATAACTGAAGCAATCACAAGCCTGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAATTCATCGAAGAGAAATACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAAGGTGAAAAGCTCCTTCAAACTCGCTCCGGCAGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAGCTGTCACAAAGCCCAAGGCTAAAGCTGCTGGGAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTATTATTATTATTGG
TARGET=723,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00551 Ps_contig_mira-and-tgicl-ass_franssen_5301
SEQUENCE=GTTCGGGGACATCATCACATTCTCTTCAATCTTCTCAATATCTCTCATTCTCTCTCATTCTCTCTCATCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACTGCTGCGGCAAAGAAGCCACYTTCTCATCCCACTTTCGCTGAGATGATAACTGAAGCAATCACAAGCCTAGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAGTTCATCGAAGAGAAACACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAGGGTGAAAAGCTCCTTCAAACTCGCTCCGGCGGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAACTGTCACAAAGCCCAAGGCTAAAGCTGCKGGAAGCCGAAAGCCAAAGCTGCGGCAAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTTATTATTATTATGGTTTGGTTTTAGAAAGATTAGAGATGAAAATTAGTGTTAGGAGGTTGTTGCTGTAAATTGTTTAGGAGAAACACATCTTGTGTTCTTTGTACTCGACACCGTCTTAATGAAATGGGTTTTCTGTTTTCCYSA
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00552 Ps_contig_mira-and-tgicl-ass_franssen_5301
SEQUENCE=GTTCGGGGACATCATCACATTCTCTTCAATCTTCTCAATATCTCTCATTCTCTCTCATTCTCTCTCATCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACTGCTGCGGCAAAGAAGCCACYTTCTCATCCCACTTTCGCTGAGATGATAACTGAAGCAATCACAAGCCTAGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAGTTCATCGAAGAGAAACACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAGGGTGAAAAGCTCCTTCAAACTCGCTCCGGCGGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAACTGTCACAAAGCCCAAGGCTAAAGCTGCKGGAAGCCGAAAGCCAAAGCTGCGGCAAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTTATTATTATTATGGTTTGGTTTTAGAAAGATTAGAGATGAAAATTAGTGTTAGGAGGTTGTTGCTGTAAATTGTTTAGGAGAAACACATCTTGTGTTCTTTGTACTCGACACCGTCTTAATGAAATGGGTTTTCTGTTTTCCYSA
TARGET=672,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00553 Ps_contig_mira-and-tgicl-ass_franssen_5301
SEQUENCE=GTTCGGGGACATCATCACATTCTCTTCAATCTTCTCAATATCTCTCATTCTCTCTCATTCTCTCTCATCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACTGCTGCGGCAAAGAAGCCACYTTCTCATCCCACTTTCGCTGAGATGATAACTGAAGCAATCACAAGCCTAGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAGTTCATCGAAGAGAAACACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAGGGTGAAAAGCTCCTTCAAACTCGCTCCGGCGGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAACTGTCACAAAGCCCAAGGCTAAAGCTGCKGGAAGCCGAAAGCCAAAGCTGCGGCAAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTTATTATTATTATGGTTTGGTTTTAGAAAGATTAGAGATGAAAATTAGTGTTAGGAGGTTGTTGCTGTAAATTGTTTAGGAGAAACACATCTTGTGTTCTTTGTACTCGACACCGTCTTAATGAAATGGGTTTTCTGTTTTCCYSA
TARGET=722,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00554 Ps_contig_mira-and-tgicl-ass_franssen_5305
SEQUENCE=CCGGGTAGGACGTCTAGGCGTTTCCAAAGTTAGGCGGCCGAGAAGGAAAGGGTTAGGTTTGGTCAGAAGACCACTGGTTCTTTGTACACCACTTCTGTCTTCTTCTTCTCCTCGTTTAAAGTTMYTCCATCTTTCCGTTAGACCTAAGGATGGACTTGGTAGTTGGTCAACACTTTTCCAGAGGCACCGCTACCTCTTATCTGCTCTCGTGCTCCTTACTATTCTATGTACTGTTTATCTTTACTTCGCCATCACTATRGGAAGTGGCTCCTGTTCTGGGTTGAGTGGAGCACAGAAGCTTCATGCCATATGGAGCTTTGGTACCAAGGTNTTTCTTACTTTTACCGAAGTTCGGG
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00555 Ps_contig_mira-and-tgicl-ass_franssen_5318
SEQUENCE=CGAACAATAGAACTGCAACAGATATTAACACCTTCCAATTCTTCATCTTGGAAGGTGGCCAATTCCCCTTCTTCCTCAACCTCCTCTTTTGAACAAGCCTCTTTCTCTTCAACCTTATCTTCTTGGCAAGCTTCATCTTCAACATCCGCTCTTCCCTCTCCTTCAATTTTGATTCTAATGGAAGCTTATCTACAGAAACAACATCACTCTCTTTCTTGCTCTCACTTTCTTCTACTCCATCTGCAACATTAGAATCAGCAGCAGCAGCAGCTGATGCAATAAAAATTGCATTTTTTCTCGGTTTCACAACAATGGGTGTCAAGATTCGAAATCCCTTCAAACAGTTGGTGGGCAGACCGATTCGTACTCTAGAAATAGGGTTTGCTGTGGGATGAAGGAAAAGGGAGGAAGAAGAGGTAACAGGGGTGGTTGTGAAGGAATTGAAGCAGAGAAGTGCCATAGTGTTTGGTTTTTTATTTTTCTTTTTCAAATGAAAAATTGGTTTATATGATGTTATGTGGTATGTTATGTTAAGGATAAGAAAGGCACATCAGACGTGTCTTCTTCCATGCTCACCACTCACCCCGA
TARGET=256,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3365
PRIMER_LEFT_PENALTY=0.142170
PRIMER_RIGHT_PENALTY=0.194358
PRIMER_LEFT_SEQUENCE=TCTTCCTCAACCTCCTCTTTTG
PRIMER_RIGHT_SEQUENCE=GATTTCGAATCTTGACACCCAT
PRIMER_LEFT=69,22
PRIMER_RIGHT=332,22
PRIMER_LEFT_TM=59.858
PRIMER_RIGHT_TM=60.194
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.3365
PRIMER_LEFT_1_PENALTY=0.142170
PRIMER_RIGHT_1_PENALTY=0.194358
PRIMER_LEFT_1_SEQUENCE=CTTCCTCAACCTCCTCTTTTGA
PRIMER_RIGHT_1_SEQUENCE=GATTTCGAATCTTGACACCCAT
PRIMER_LEFT_1=70,22
PRIMER_RIGHT_1=332,22
PRIMER_LEFT_1_TM=59.858
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.3782
PRIMER_LEFT_2_PENALTY=0.202525
PRIMER_RIGHT_2_PENALTY=0.175636
PRIMER_LEFT_2_SEQUENCE=GCTCTTCCCTCTCCTTCAATTT
PRIMER_RIGHT_2_SEQUENCE=TCTTCTTCCTCCCTTTTCCTTC
PRIMER_LEFT_2=147,22
PRIMER_RIGHT_2=414,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.3966
PRIMER_LEFT_3_PENALTY=0.142170
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=TCTTCCTCAACCTCCTCTTTTG
PRIMER_RIGHT_3_SEQUENCE=CACCAACTGTTTGAAGGGATTT
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=349,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.3966
PRIMER_LEFT_4_PENALTY=0.142170
PRIMER_RIGHT_4_PENALTY=0.254467
PRIMER_LEFT_4_SEQUENCE=CTTCCTCAACCTCCTCTTTTGA
PRIMER_RIGHT_4_SEQUENCE=CACCAACTGTTTGAAGGGATTT
PRIMER_LEFT_4=70,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=60.254
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_00556 Ps_contig_mira-and-tgicl-ass_franssen_5335
SEQUENCE=CGTTTATTTCATCTTAATATATCATTGAGTATCAATAAGCTTAGATCATCCATCATATACAATTACATAATACGCACTAATATAGAAAGTACAATGGAAGGATAAATTGCTGAAAATGTGAGATTTGAGAGAATCCCAATTGAAATCTTACCATCCCCAGAAGGACCAATCAGGAAAAGGTATATCAAAATGTTCCTTTGTGGTACAATATCGAAAGAGTGGCAAAGGCTTTGAATTATGTTCAGTTTTGTTAATACTAGGCTTATCCATGCAATCAAACATCAAATCCACATCAGGAACCAACCCAGGGTACTTCCTCAAAAGCTGCAACAAACCCCAAACAGTAAACATAGCCCTACTCTGAACACAAGCATAATACCAATCAACAAACATCTTCCCTCCAACAATCACCACTCTAAAAGCCGCATACTTTTGCGCTTCCGCCAAATGCCCCTCCGAAATTCTGGTTTTCATCCACGGCTCCAGATCTCTCCGGATCGCCTTGAAAAAATCAGGACAAGTCGGCGCTTTCGCTTTCGCATCTCCGGCAGCGTAACCGAGTCGTCCGCTGCCACCGGAGACCGCGTTACGGCATGTGAGATATGAACACTGTATGATTTTGTAGGCTCTGCTCTGACGAGTCTCTTCGTCGAATGGTTTCGTAGGGAAAACGTGCCACGGCGTTGGTTCTAGGTTGTGACCTACCACGGTTCCGGTTCGGCTGACAACGTCGTCCACCTTGTAGAGAAGAAGGCCAGTAAGAGAGAAGAAAGAGAGAGCGATGACACATGGAAAGAGATAAGTTGGGGTACGAGGAGAGTGTTTGGGAGAGAGACCCATGATAGAAGAAGAAGAAAAAGTGAAGAAGAAGAAAGAATTGTTGTGAGTGTTTTGAGACATTGTGCTGTGAGTGTKWGWAAATTTGAGGTGACTTGGAAAATATGGACACTCAATTCACGTGACGGTGGAGACCACGTGTGTGAAAAAAGTC
TARGET=844,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00557 Ps_contig_mira-and-tgicl-ass_franssen_5335
SEQUENCE=CGTTTATTTCATCTTAATATATCATTGAGTATCAATAAGCTTAGATCATCCATCATATACAATTACATAATACGCACTAATATAGAAAGTACAATGGAAGGATAAATTGCTGAAAATGTGAGATTTGAGAGAATCCCAATTGAAATCTTACCATCCCCAGAAGGACCAATCAGGAAAAGGTATATCAAAATGTTCCTTTGTGGTACAATATCGAAAGAGTGGCAAAGGCTTTGAATTATGTTCAGTTTTGTTAATACTAGGCTTATCCATGCAATCAAACATCAAATCCACATCAGGAACCAACCCAGGGTACTTCCTCAAAAGCTGCAACAAACCCCAAACAGTAAACATAGCCCTACTCTGAACACAAGCATAATACCAATCAACAAACATCTTCCCTCCAACAATCACCACTCTAAAAGCCGCATACTTTTGCGCTTCCGCCAAATGCCCCTCCGAAATTCTGGTTTTCATCCACGGCTCCAGATCTCTCCGGATCGCCTTGAAAAAATCAGGACAAGTCGGCGCTTTCGCTTTCGCATCTCCGGCAGCGTAACCGAGTCGTCCGCTGCCACCGGAGACCGCGTTACGGCATGTGAGATATGAACACTGTATGATTTTGTAGGCTCTGCTCTGACGAGTCTCTTCGTCGAATGGTTTCGTAGGGAAAACGTGCCACGGCGTTGGTTCTAGGTTGTGACCTACCACGGTTCCGGTTCGGCTGACAACGTCGTCCACCTTGTAGAGAAGAAGGCCAGTAAGAGAGAAGAAAGAGAGAGCGATGACACATGGAAAGAGATAAGTTGGGGTACGAGGAGAGTGTTTGGGAGAGAGACCCATGATAGAAGAAGAAGAAAAAGTGAAGAAGAAGAAAGAATTGTTGTGAGTGTTTTGAGACATTGTGCTGTGAGTGTKWGWAAATTTGAGGTGACTTGGAAAATATGGACACTCAATTCACGTGACGGTGGAGACCACGTGTGTGAAAAAAGTC
TARGET=862,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00558 Ps_contig_mira-and-tgicl-ass_franssen_5352
SEQUENCE=ATCTCCGACAAAGTTACTAATACTTTGTAGGTAAAGGTAACGGAAGTTACTTCTTCTTTCTAATTGGGAACGTTAGGTTCGGTATGTCGTTTGGTACGGCCGGAACGGAAAGGAAAGGAAAGGAAAATAAGGTTCGAATAGGTGGTTTGGTACGGAAGGAAAGTACTTTTCTATTTTCTTTTGGGTCGTGTTTTCTTTCTTCCAATCGGTGACCCAATTGAAGGAATGAACCACCGTCTTCTTCATCTCGATGATGGGTTCAGTGAGGAACAGGTTAAGGTTAACCATGATATTGATGATGATGATGATGAAGAAGAAGACGACGATGATGATGATGAAGTATTTATTTGGATGATGAGTCTTTGTTCCAGTGGGATAGTARGGAAACTAAAGTTGGGAAANACAGGAAGAATAGGAAGGTAAAAGGTTTAGGGGGTAAAACGGTTTCAATACAAAAGATAGAAACTAAGTTACGG
TARGET=107,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00559 Ps_contig_mira-and-tgicl-ass_franssen_5352
SEQUENCE=ATCTCCGACAAAGTTACTAATACTTTGTAGGTAAAGGTAACGGAAGTTACTTCTTCTTTCTAATTGGGAACGTTAGGTTCGGTATGTCGTTTGGTACGGCCGGAACGGAAAGGAAAGGAAAGGAAAATAAGGTTCGAATAGGTGGTTTGGTACGGAAGGAAAGTACTTTTCTATTTTCTTTTGGGTCGTGTTTTCTTTCTTCCAATCGGTGACCCAATTGAAGGAATGAACCACCGTCTTCTTCATCTCGATGATGGGTTCAGTGAGGAACAGGTTAAGGTTAACCATGATATTGATGATGATGATGATGAAGAAGAAGACGACGATGATGATGATGAAGTATTTATTTGGATGATGAGTCTTTGTTCCAGTGGGATAGTARGGAAACTAAAGTTGGGAAANACAGGAAGAATAGGAAGGTAAAAGGTTTAGGGGGTAAAACGGTTTCAATACAAAAGATAGAAACTAAGTTACGG
TARGET=294,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00560 Ps_contig_mira-and-tgicl-ass_franssen_5352
SEQUENCE=ATCTCCGACAAAGTTACTAATACTTTGTAGGTAAAGGTAACGGAAGTTACTTCTTCTTTCTAATTGGGAACGTTAGGTTCGGTATGTCGTTTGGTACGGCCGGAACGGAAAGGAAAGGAAAGGAAAATAAGGTTCGAATAGGTGGTTTGGTACGGAAGGAAAGTACTTTTCTATTTTCTTTTGGGTCGTGTTTTCTTTCTTCCAATCGGTGACCCAATTGAAGGAATGAACCACCGTCTTCTTCATCTCGATGATGGGTTCAGTGAGGAACAGGTTAAGGTTAACCATGATATTGATGATGATGATGATGAAGAAGAAGACGACGATGATGATGATGAAGTATTTATTTGGATGATGAGTCTTTGTTCCAGTGGGATAGTARGGAAACTAAAGTTGGGAAANACAGGAAGAATAGGAAGGTAAAAGGTTTAGGGGGTAAAACGGTTTCAATACAAAAGATAGAAACTAAGTTACGG
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00561 Ps_contig_mira-and-tgicl-ass_franssen_5382
SEQUENCE=AGCAGTGGGATCAACGCAGAGTTCGGCTAGGGTTCTAGAGCAGGAAACTCATAATAAGAAGTTGAGACAAAAAAATTATGAGATCTGCTGAATAACTATCTTCAATGGTGGAAATTACTTCTGTTCTTTCAGATATGATATGGCAATGGACAATGTTCAGAGATATGCACCTGACTTGGATCATCAAGTTTCTTTTGAAATCACATGAAACTTGAGTTTTCCTTTTTGAACTTTTGGGTGAACGTGTAGTATATGTGGCGCAGATGTTATTGTATTTTACTTTTTGGCTACTCTTCAAGGTGAAGAAGGTATTGATTGTGCAACTTTGTTGAATGATTTGTGCCTTCATAATATTCGAAAGGTCCTGATGTTCTTGTCTCCCTGCACCACCATTTACTTCATGTAATGATATGCCAACTGTCTTCTATATTGTGTAACTCTTTCTTTCTTTTAGCTCTTCA
TARGET=439,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00562 Ps_contig_mira-and-tgicl-ass_franssen_5388
SEQUENCE=AGATTTGTAGAGAGGAAGGTGTGGAACAGGTTTTTGTTGATGCTGCTCATTCTATTGGGTGTACTGATGTTGACATGCAGGAGATTGGTGCTGATTATTACACTAGTAATTTGCACAAGTTGGTTTTTTTGTCCGCCTTCGATTGTCGTTTTTGTACTCGAAGAAAAAACACTAAACTACTGGTGGTGGTGGTTTGCATCATCCTGTGGTGTCTCATAGAGTATGGAAATGGGTTGGCTGTAGGAAAGTGCTTGGAGTTAGGG
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1368
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.050443
PRIMER_LEFT_SEQUENCE=GTGTGGAACAGGTTTTTGTTGA
PRIMER_RIGHT_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT=18,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.950
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.1920
PRIMER_LEFT_1_PENALTY=0.141511
PRIMER_RIGHT_1_PENALTY=0.050443
PRIMER_LEFT_1_SEQUENCE=CTGATGTTGACATGCAGGAGAT
PRIMER_RIGHT_1_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_1=63,22
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=59.950
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.2160
PRIMER_LEFT_2_PENALTY=0.165541
PRIMER_RIGHT_2_PENALTY=0.050443
PRIMER_LEFT_2_SEQUENCE=TCGATTGTCGTTTTTGTACTCG
PRIMER_RIGHT_2_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_2=138,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=59.950
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.2400
PRIMER_LEFT_3_PENALTY=0.189529
PRIMER_RIGHT_3_PENALTY=0.050443
PRIMER_LEFT_3_SEQUENCE=AGGTGTGGAACAGGTTTTTGTT
PRIMER_RIGHT_3_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=59.810
PRIMER_RIGHT_3_TM=59.950
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.2808
PRIMER_LEFT_4_PENALTY=0.230329
PRIMER_RIGHT_4_PENALTY=0.050443
PRIMER_LEFT_4_SEQUENCE=TTGATGCTGCTCATTCTATTGG
PRIMER_RIGHT_4_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=60.230
PRIMER_RIGHT_4_TM=59.950
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_00563 Ps_contig_mira-and-tgicl-ass_franssen_5392
SEQUENCE=CTTAAACTTACATAATTATCTGCATGTTAAAGACAATATAAAAATAGAATAACCYWCACACTAAATCAAACTCCATTAAACTAAAATAAATCTAAGCAAACTAATATTATGACTATTTACAAATTATCTACAGAGTACCGGCTAAAACATGATAAAACAATCACAGTCCAATTTAATTGAAATTTATATTCCACAACTTTAACGCAATTGAACTTAAGATCGAAAATTTACCGACTCCAGATTTGAACTTGTTGACCTCAGACCGACTAATGCACGAACAAAAGTCAACGTAACGCGGAGGTTGTCGTGTGTATCTGTGAATGAATCTTTGTAAAGGGAGGTCGGAGAAATTGCAGATCGGAGGGTATATGGCGGTCCGACGATGAAACGGGGGCAAAGGAGCGATTTAAGGTGTTTACGGTGATGGTATTTTCGCCGGCTACAGTTAGGAGTAGATTTTGAGTTTTACAAGTTTGAGGATGTTGTTCCACGGCGAGTTGAAATCGGAGGCGGTTTTGAGTTATCGGTGAAACGCAACGGCAAAGGGAGCTTTATGGAATCCGCAGTGGATTTGACGGTGGCGGAAGATGAAGAGGTTCGTGGTGGTGGGGATGAGTGATACCGGCGCAATCGACGAGTCGGCGGCGACGGAGGTATGTGTGTCGTGGCGGCGGAGGATTTCACCGGCAATGGATGGAGTTTGGTCGGTGGTCGCCGGAACACGGAGAGGGTTTGAGCAAGCTTTTCGCCGGAATTGGTGGCGGATGGAGAGAGGATGGTGTTTGGTTTTTAAGGTCTGTTTTCTTTGTATTTTTGTTTGTGAGATTTTCAAAGAATAGGTAGCTTTTGGTTAAGGAGGAGAGATTGGGTGGGAAAGAAGAGAGGACGTGTGAATCTGTGTGTCSCCTTGTTCTTTTTGTTTTTCTTTTTAATGTACAGAGAGAGAGGGGTAACGTGGAAAGGTGAGGTAG
TARGET=938,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00564 Ps_contig_mira-and-tgicl-ass_franssen_5414
SEQUENCE=GGTTTTTAGTTTTCTTAGTACTTGAGGGTCTTGCGCGTAATTTTTGTAAAGCCTGTAATAAGGTGGTGGTGGTGGATATGTTGCCGTTGCCATAACAAAAAATCTTCCAATCAAAAACCGTTAAAGATCATACATGCATCTCTACTTCAAGATTTGACAGAGTTGTGTAGATGGAGGGCCGGCCGCCGGTGTGATGTCTGCGGCTGAGAAGCTGACTGGTGATGTGCTGGGCAGCTGTCGGCT
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4316
PRIMER_LEFT_PENALTY=0.954958
PRIMER_RIGHT_PENALTY=0.476630
PRIMER_LEFT_SEQUENCE=CGCGTAATTTTTGTAAAGCCTG
PRIMER_RIGHT_SEQUENCE=ACATCACCAGTCAGCTTCTCAG
PRIMER_LEFT=33,22
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=60.955
PRIMER_RIGHT_TM=59.523
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=1.5216
PRIMER_LEFT_1_PENALTY=1.044969
PRIMER_RIGHT_1_PENALTY=0.476630
PRIMER_LEFT_1_SEQUENCE=TCTTAGTACTTGAGGGTCTTGCG
PRIMER_RIGHT_1_SEQUENCE=ACATCACCAGTCAGCTTCTCAG
PRIMER_LEFT_1=12,23
PRIMER_RIGHT_1=224,22
PRIMER_LEFT_1_TM=59.955
PRIMER_RIGHT_1_TM=59.523
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=1.7940
PRIMER_LEFT_2_PENALTY=1.317328
PRIMER_RIGHT_2_PENALTY=0.476630
PRIMER_LEFT_2_SEQUENCE=GGTCTTGCGCGTAATTTTTGTA
PRIMER_RIGHT_2_SEQUENCE=ACATCACCAGTCAGCTTCTCAG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=61.317
PRIMER_RIGHT_2_TM=59.523
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=2.0004
PRIMER_LEFT_3_PENALTY=0.954958
PRIMER_RIGHT_3_PENALTY=1.045432
PRIMER_LEFT_3_SEQUENCE=CGCGTAATTTTTGTAAAGCCTG
PRIMER_RIGHT_3_SEQUENCE=CCCTCCATCTACACAACTCTGTC
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=177,23
PRIMER_LEFT_3_TM=60.955
PRIMER_RIGHT_3_TM=60.045
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=2.0210
PRIMER_LEFT_4_PENALTY=0.954958
PRIMER_RIGHT_4_PENALTY=1.066065
PRIMER_LEFT_4_SEQUENCE=CGCGTAATTTTTGTAAAGCCTG
PRIMER_RIGHT_4_SEQUENCE=GCCCTCCATCTACACAACTCTG
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.955
PRIMER_RIGHT_4_TM=61.066
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_00565 Ps_contig_mira-and-tgicl-ass_franssen_5415
SEQUENCE=GAAAGTTGGTTCGGAAAAACCCTAGACGGGGGAGTAGTGGAGTTTGAGTTGAGTTCAGGGTTTACTTTCAATTTCAATTCAGTACCAACTAAACCTATTGACTCTCTCTCTCTCTCTCTCTCTGCAATGGCAGTTTCAACGGAATTTCACCTTCCACGTCTCTCCGTCGTCGACTTTCCCTCTCTTCTCCAAGCTATGGCAGCCGACACTCTTCTTGCCGCTGCTCAATCTCTCGCTCTCATTGGATATCCGCTGGCTAATTTCACCAGTTTAGTGTCTCCTAAA
TARGET=102,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1693
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_SEQUENCE=CATAGCTTGGAGAAGAGAGGGA
PRIMER_LEFT=38,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.3401
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.194358
PRIMER_LEFT_1_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_1_SEQUENCE=GGATATCCAATGAGAGCGAGAG
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.5146
PRIMER_LEFT_2_PENALTY=0.145769
PRIMER_RIGHT_2_PENALTY=0.368866
PRIMER_LEFT_2_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_2_SEQUENCE=GCTTGGAGAAGAGAGGGAAAGT
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.146
PRIMER_RIGHT_2_TM=60.369
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.5360
PRIMER_LEFT_3_PENALTY=0.145769
PRIMER_RIGHT_3_PENALTY=0.390220
PRIMER_LEFT_3_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_3_SEQUENCE=TAGCTTGGAGAAGAGAGGGAAA
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=60.146
PRIMER_RIGHT_3_TM=59.610
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=0.6347
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.488948
PRIMER_LEFT_4_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_4_SEQUENCE=AGCTTGGAGAAGAGAGGGAAAG
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=60.489
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_00566 Ps_contig_mira-and-tgicl-ass_franssen_5416
SEQUENCE=AAGCATGGTATCAACGCAGAGTGTTTGGTGGCAATGGAAAGACTGGCAGAGATTGAGAGGGCTGAGAGGAAAATTGAAACTCTTGGGAGGGAGAAAGATAATCTGGAAACTGAATTGCAGAGAGCAACGTATTCAGAGAAGGATGCACTTACCAGAGTTGCGAAGCTTGAGGAGAAAGTGCAACAGAGAGAGAAGGATTTAGAAGCACTTCTGGATAAAGATAAAACACACAGGAAAAATAACGCTGAGATTCTTGAACAACTTTTGGAAACAGAACGTGAAGCACATACACAGGCAAATAACCGGGCCGAGGCTCTCTCTCTGCAGCTACAGTCTGCACAAGCCAAAATTGACTCTCTACATCAAGAGCTGACGAAGTTTCGACTGAATGAAACATTAGACAGTAAGCTAAAAACATCCGATGGTAAGCGATTGAGGGAAGAAAATGACATTGGTGTTGATTCTGTACAAGACATGGATGCGAGCCCTAGAATTTTAAGGGGAACTAAAAGAGCTAGGAGTACATCCAGTCCTAAGTACACTCAGCCAGAAGATGGTGGTTCAATCTTCGAGGGCGGAGAGGATAATCTTAGTCAGCAAACAAATGAAGAGAATTATAAAAAGTTTACCGTCCAGAAGCTTAAGGTAGAACTGACAAAACATAACTATGGTGATCAGCTGCTTCGGTTGAAGAATCCCACCAAGAAAGATATTCTTGCTCTGTATGAGAAGTTTATTCTTCAGAAGGCATAACAGGTTTTGAAAAGATGTGTATTAGTATGCTGCATGTAATCACTCCGTCTAAGTCTCTCTAAATATCATTGATGCCGGGTTTATGAGCTGTGAAATATCGAATTAATTTTAACTTTTTTTTGTTTGTTGAGTTCTACTTGTGTTTGCTAGCAATGATTATCATTTAGGCCATACAGGGTTTTGATGGCACGATCTTTATTTGTTGATTGTTTGAGTTGTATTTAATACATCTGTACAGTAGCATTTAGTCGTATTACTCATAACTCTAACATTGTTTCCTATAGTTAGTATGAACTAATTACTAGAAAAAAGT
TARGET=314,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2291
PRIMER_LEFT_PENALTY=0.165541
PRIMER_RIGHT_PENALTY=0.063537
PRIMER_LEFT_SEQUENCE=CACACAGGAAAAATAACGCTGA
PRIMER_RIGHT_SEQUENCE=TCCCCTTAAAATTCTAGGGCTC
PRIMER_LEFT=226,22
PRIMER_RIGHT=503,22
PRIMER_LEFT_TM=60.166
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.2347
PRIMER_LEFT_1_PENALTY=0.171146
PRIMER_RIGHT_1_PENALTY=0.063537
PRIMER_LEFT_1_SEQUENCE=CAGAACGTGAAGCACATACACA
PRIMER_RIGHT_1_SEQUENCE=TCCCCTTAAAATTCTAGGGCTC
PRIMER_LEFT_1=271,22
PRIMER_RIGHT_1=503,22
PRIMER_LEFT_1_TM=59.829
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2611
PRIMER_LEFT_2_PENALTY=0.165541
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=CACACAGGAAAAATAACGCTGA
PRIMER_RIGHT_2_SEQUENCE=ACACCAATGTCATTTTCTTCCC
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=457,22
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.2664
PRIMER_LEFT_3_PENALTY=0.170871
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=TGAGGAGAAAGTGCAACAGAGA
PRIMER_RIGHT_3_SEQUENCE=ACACCAATGTCATTTTCTTCCC
PRIMER_LEFT_3=167,22
PRIMER_RIGHT_3=457,22
PRIMER_LEFT_3_TM=60.171
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.2818
PRIMER_LEFT_4_PENALTY=0.060824
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=AAGCTTGAGGAGAAAGTGCAAC
PRIMER_RIGHT_4_SEQUENCE=CACCAATGTCATTTTCTTCCCT
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=456,22
PRIMER_LEFT_4_TM=60.061
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_00567 Ps_contig_mira-and-tgicl-ass_franssen_5418
SEQUENCE=TTCATTCACTTGAATATTCATTTTAATCCATTGATTGAAGTTGCTTCACTTTGCATTAGAGTTCCAAGAGAATTTGGTGTGCTAATTTTGAGATTGTGACATAATATTTTGATGTATTATTATAGAGAAAGCACAAGAAATACAATTCCTTTCTTTCTTAGGAAAAAAATCYTTCTAACTTCATAGTATGATGAAATTGAATTTGTGGTTGTAAATGTTGATATGATCTTACCGAATATGCGACGAATATGGATTCGCTACAAAATTGAATCTAAACAATATGATATGATATGAATCTTTAGTGTGTATCTATTTGGAGTTTGGACAAT
TARGET=279,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00568 Ps_contig_mira-and-tgicl-ass_franssen_5437
SEQUENCE=AAGTACTAAAATTCAGGAATTTATATATATATATACTCAACACCAGCATTTGTTTACTTGTTACGTTTCCTGCAAATCATCAAACAACCTATGATTTTGTCTTAGTGAGGATGAGATGTYAACTTCATAACAGTTGTTCAAACTTTAAAACCTATGTATTTTGCTTAGTGAGGATAAGTATTTTGCTTAGCGAGGATGGGTATTTTAACTTCAATAAAACAGCTTGACTGTCC
TARGET=22,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00569 Ps_contig_mira-and-tgicl-ass_franssen_5442
SEQUENCE=ATCGCCGTCGATGACGTCAGAAGAATCCGTGTACTTACGTACGGCGYGCTTGGAGGCTGGGATCGGAGAAAAATGGACTCACGGTGGTGATAGGAAAGTTCTCAACCTTTTCATATGAGGACTCTCTGGGTTTGTGTTGCGTTTGCGTGTATCACCTCACGCGCGCGTCGTAACGTTCCTTTGTTTTGCTTACAATACTCATTGTGGGGGAAGGGAGAAAACGGTTTTGTAGTTGGACGAGTTTTCGTGAGTTACCGGTTCAGGATTTCTCTCGAGAGAAAAATGATGGTTCGTCGGTGATTTTTTTTCTTTCTTTTCAGTCTTTTTGATTTTATTTTATTTTCTAGCAAATATGAGACATGTAGTAAACCTGTAAGCGTGGAATATTAATATTAAATCATATGAAATAAAAATAAAGAGAAGTTTTATTTAAATTAAAAATAAACG
TARGET=329,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00570 Ps_contig_mira-and-tgicl-ass_franssen_5444
SEQUENCE=ACCTTCGTTCTCTGGGTTTCTACCCATACTCATTGCCTCACACAATCTCCTCCAAGTACATCCATGAACTGTAACCACGCCACTCTCCTTTGTCTTTCTCTCCCACCACCACCACTGAATTCAAATCGTGTCATTTTCCCAAAATGCCATTCTGCAATTCCCTTATCCAACAAAACCCTTTTGTGCAAACCGAAGCTGAAGAGAAAAACGATTCTTCTTAGAATCAATGCTTCTTCAAAGGATGATAATGCTCGATCACCAAATTGGTCTAAGTGGATCCCCACAGGCTCTTTCGCTGCTGAATTAAAGTTTTCAGATTGATTTCCAGTGCCTACCGCTACGTCCCATTGGACTAGTTTGTATCCTTCCCCCCACCTACGTTTCTTCACTCTA
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2411
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=ACATCCATGAACTGTAACCACG
PRIMER_RIGHT_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT=57,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.4075
PRIMER_LEFT_1_PENALTY=0.386286
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=TACCCATACTCATTGCCTCACA
PRIMER_RIGHT_1_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.386
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.4294
PRIMER_LEFT_2_PENALTY=0.408180
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TCTACCCATACTCATTGCCTCA
PRIMER_RIGHT_2_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=59.592
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.5034
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.283555
PRIMER_LEFT_3_SEQUENCE=ACATCCATGAACTGTAACCACG
PRIMER_RIGHT_3_SEQUENCE=GGGTTTTGTTGGATAAGGGAAT
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.5143
PRIMER_LEFT_4_PENALTY=0.493142
PRIMER_RIGHT_4_PENALTY=0.021195
PRIMER_LEFT_4_SEQUENCE=GCCACTCTCCTTTGTCTTTCTC
PRIMER_RIGHT_4_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=211,22
PRIMER_LEFT_4_TM=59.507
PRIMER_RIGHT_4_TM=60.021
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_00571 Ps_contig_mira-and-tgicl-ass_franssen_5455
SEQUENCE=GGGCCTCAGTAGTTGAAATATCTCTCTCTCTCTCTCTCTCTGAGCAAGTTGTTTTCTTTAATCTTTCATATCCCAAATCTAATCTGTATCTAGCTTCTACCATTCTATACCAAAAAAATCAGAGTGAACTAACATAATTGTGAAACTAAGAAAATTAATTTAAAGGAACTGATATGAAGTGAGATTATTTTTACAAACATGGAAGGAAGGAATACAAAGATGGATAACAAAAAAGAAAAACTA
TARGET=21,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00572 Ps_contig_mira-and-tgicl-ass_franssen_5455
SEQUENCE=GGGCCTCAGTAGTTGAAATATCTCTCTCTCTCTCTCTCTCTGAGCAAGTTGTTTTCTTTAATCTTTCATATCCCAAATCTAATCTGTATCTAGCTTCTACCATTCTATACCAAAAAAATCAGAGTGAACTAACATAATTGTGAAACTAAGAAAATTAATTTAAAGGAACTGATATGAAGTGAGATTATTTTTACAAACATGGAAGGAAGGAATACAAAGATGGATAACAAAAAAGAAAAACTA
TARGET=201,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00573 Ps_contig_mira-and-tgicl-ass_franssen_5467
SEQUENCE=AGTAATAAGTTATAACAAATGATTTAAAGAAGTAAAAATTTAATGTGTGATTATTAAGCTCTGAATTGAGAGTGGAAGAGTCTGTGTGTGTGCGCGKTKGGGTTCTCTACTTCCTT
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00574 Ps_contig_mira-and-tgicl-ass_franssen_5468
SEQUENCE=ACTTTTGKAACTCTTGTTCTTTTTCAATATATATAATTTCAAGTTTACACCATAAATATAATTTGAAGTTTATACAACAATATAACATATATGGTATATCCTAGCTCACACCTCACATCAACAAAATAATAACATATAAAAAAATTACTATAAAATAAATAAATGGTATTTAACTTACTAAAATAACAAAAACATCCCTAACAAGACAAGAAACACATAAAACTTGTTGTAAAGTTGTGCATACTTTAACACTGAATAAGAACCAGATTTCTCATCACTTGTTTTATCATCATCTTTGTCTTCCTTAGGATTGTTAAAATTGGAAGGAACAGTTGTGTCCGGTGCCGAGTCATCGTCACCGGAAACCGGACTAGCAGGAGAAGGAGAATCATCTGGTAGAAGACTCTTTGGCAACCCTAATCCATGAGTCACATTTATTCTGAAATGTTGTCCATTTTTGCATTGCTCGCCATCATAATCACTCGAGAAGAAATATGTTGGTCCTTCTTTCAACAAAGGAACTGCCACTGTGAGTGGAGTTACTTCACCGTTGGGTTGAGAACCTGATGACCATTGGAGTGTGTCTTTGTCCTGTGCATCATCGTAGTCACATTGTTTGTATGTTGTGAAATTGTATGTTTGAACAATAGTGTGGTTGCTGTCTGTATTGAAAATGAGAAAATCTCCCAAGCTGAATTGTTTTTTGGCAACCCATTTCTGATAATTAACAGTTGGTTTTTCAAGTTTGTCAAACCAGCCTAAAGAATCCCCCACTGTGTAATTTTTGTAAGCTCCAACAGATCCTAAGAAACATGAAAGAAAAAGACAAGAAAGAATTGAGAATAGAAGGCATGTTGATGATGATGATGATGATGATGAGTTTCTTCTATATTCCATTATTCTTGGTGTGTGTGAAATTATTGTGAATCGAAGCAAACTAAGTAAGTAGCTTTTTTATGAGTTAGTGGATGAGAATGTAGCATTCATATGCTGCCAGCTATGGTGTCTCAAGTGGTGTATATTTAAGAAGCCTTGCTTTTCTAATGAATTACTAAAATATACAATCTGTTGTGTTGGTTAGTTCC
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00575 Ps_contig_mira-and-tgicl-ass_franssen_5468
SEQUENCE=ACTTTTGKAACTCTTGTTCTTTTTCAATATATATAATTTCAAGTTTACACCATAAATATAATTTGAAGTTTATACAACAATATAACATATATGGTATATCCTAGCTCACACCTCACATCAACAAAATAATAACATATAAAAAAATTACTATAAAATAAATAAATGGTATTTAACTTACTAAAATAACAAAAACATCCCTAACAAGACAAGAAACACATAAAACTTGTTGTAAAGTTGTGCATACTTTAACACTGAATAAGAACCAGATTTCTCATCACTTGTTTTATCATCATCTTTGTCTTCCTTAGGATTGTTAAAATTGGAAGGAACAGTTGTGTCCGGTGCCGAGTCATCGTCACCGGAAACCGGACTAGCAGGAGAAGGAGAATCATCTGGTAGAAGACTCTTTGGCAACCCTAATCCATGAGTCACATTTATTCTGAAATGTTGTCCATTTTTGCATTGCTCGCCATCATAATCACTCGAGAAGAAATATGTTGGTCCTTCTTTCAACAAAGGAACTGCCACTGTGAGTGGAGTTACTTCACCGTTGGGTTGAGAACCTGATGACCATTGGAGTGTGTCTTTGTCCTGTGCATCATCGTAGTCACATTGTTTGTATGTTGTGAAATTGTATGTTTGAACAATAGTGTGGTTGCTGTCTGTATTGAAAATGAGAAAATCTCCCAAGCTGAATTGTTTTTTGGCAACCCATTTCTGATAATTAACAGTTGGTTTTTCAAGTTTGTCAAACCAGCCTAAAGAATCCCCCACTGTGTAATTTTTGTAAGCTCCAACAGATCCTAAGAAACATGAAAGAAAAAGACAAGAAAGAATTGAGAATAGAAGGCATGTTGATGATGATGATGATGATGATGAGTTTCTTCTATATTCCATTATTCTTGGTGTGTGTGAAATTATTGTGAATCGAAGCAAACTAAGTAAGTAGCTTTTTTATGAGTTAGTGGATGAGAATGTAGCATTCATATGCTGCCAGCTATGGTGTCTCAAGTGGTGTATATTTAAGAAGCCTTGCTTTTCTAATGAATTACTAAAATATACAATCTGTTGTGTTGGTTAGTTCC
TARGET=856,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00576 Ps_contig_mira-and-tgicl-ass_franssen_5477
SEQUENCE=GAAAGTGCTTTTCATTTCTGCGTTAGAATCTGAGAGAAGAAAAAGAGAAGAAGAAGAAAGAGGAGACACAGACACAAATCAATCAACTATAGGTATYTTCACGTCTGTGGCTCCTCTTAATATAAAAGCTAGTTAGTTTCTGGGTTAAAGAAAATTTAAAGGAAAAGATGGATTAATGAGAGTAATAGTTGGTAGTTGGGAGGGTGTAACTACTATCCACTATTTATTTGGTTGGTTTAATTGGTTACGAGCGKTS
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00577 Ps_contig_mira-and-tgicl-ass_franssen_5480
SEQUENCE=CGTTCAGATAGTTTTCTACATATATATATATAAATACATACAAGAATCCAYWAAAGTAAAACCTTACACGTATATAAATACCATAAGAGCTTATTTACTAACACATACTAAAAGACTCATAAAAGATATTCCTACATAATAATAATCTACCCAGAATCTCACTAGGCAGCTTCAGCAAAACCAAGTTGAAGATTTCCATAGTCAAAGATAGTGTGATATACCCTCAATAAAAATATCACCAAGAATCCATAGAGGACCCCTTGGAGGAGGAATGTACAAAACGACAAATAAAACCCAACTAAAGACAGACTTGTGTAATACCTTCTCCGGTTTTCA
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00578 Ps_contig_mira-and-tgicl-ass_franssen_5482
SEQUENCE=TCCACCACCACCACAAACTCCACAAAATCACTGGATCTCATCAAAGCAAAAACGACAAAATCCACCGTCACAAAAACAGATCAACAAGAATCAAAGAAACCAAACAAGAAACAAACACCACCGACTTGGTTCTTCGACGACGAAGACACCGATTTCGTTTCAGACTTCAAAGATCTCCCAATGAGATTCCAACAAACACTAATCCCAGACCTCGAAAAAATCTCAACAACCTCAAAAGCCTATATAACAAAAACCAACCAACAAATCACAAAAAAATTTCAAACCTTACGTCGGAAACAAATACGCACCAACCATCGCAACTTTACNCTCTTGCACCTGTCGTTTTAC
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00579 Ps_contig_mira-and-tgicl-ass_franssen_5538
SEQUENCE=GTTAACAAAAATAAAGCCCGACATTTCATTCATTCATTATAAAAAACCAAAGAGTTCGCATAATAGATAGATCCAGCACAAAAAACCCCTAAAAAACTAATTGCAAAAA
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00580 Ps_contig_mira-and-tgicl-ass_franssen_5545
SEQUENCE=GGWKAAATTWGACTTTTAATTGAATTATTACGGTCGTTATGGAGTAAAAACAATAATAGTTACAATAGTACATTGACAATAATATTCTCCCATAGTTCAGAAAAAAAAATTAGAATGTGGTCTCATCATGCTAAGAAATTGATATTGAGTACATAAGGACACCTTAAACAAGAAAATTGGTAGAAATCAAGGGATTGGGAAGAAATTACTTTTATATAGATATAGAAACCTGTTATTACAAACCAAGGAAAACATTAGTATTTCCAAGACATTCCTCGAGCAATCAGATTACCAATCAGATTACTCAGTATCACTTTGTATCTCGCGCTACCTATACTTAAGACACATATTTCTTCAGCTTCCTCCACTATTTCTTTTCTCAAAATGATGAATCCCAAAAATAAATCAGAGAATAATAAAAGCACCTAGAATGCTGCCCATCCTGAAGCGGCTGTTGAGCCCGGAGTGGTTGATGAGGGAAGATTCTTCTGTAATTGAGAAAGATCGGATAGAGAATCAGTAGTATTATGTCTCACGCCTTCGTGTGTCCCTTTAACACTAGATCCAGTACTGGTAGTAGAAGCAATTCGGGCAGCAACAGGATCATTTGGTGGAGGTGGAAGAGCAGATCTGATTTTCCCTGCACCACTTGGTGGAGGAGCAAGGTTCAGTACTTTTGGCTTTGGAGTTGCTATGTGAGCACCAGTTAGTCCAGCTGCTGAAAGCATGCCAGCTCCCCCAGCCGCCTTATGTTTCACATTAATCCTGATGGTTTCCCCCTCCTTGAGCCTATGATTTACAGCAGGGTGAATGTCGATCTGAGAATCCTCTGAGGCATCGGAGTTACCGGACTCCTTGTCATGCTCTCGCCGGACATACTTTTCATGATCAGATAGTGCAACATTGAAATCAAAAGCTTCGTTCCTCTCCGTGAACCCTAATCCAATGAATGCGTGCTTACCTTGACCATCTTCGATCTTGAGGACGAAGTAACGAGACGAGTCGAGAACCGGTTCGACAGAACCCTCACGCTGACCGGGGAACACGAAGCACGCGGCAAAGAGATCGCCGGAGTTTGGATCTTCTAACCGGATCTCACATCGGTCCCGACGTGATACTACCCGGAGGCGTCCCGACCAGATCTTGTCGGATTGAAGCCACTCACCGCACTTGTAGCCGCCGGAGGTTGTTCGCGGCGGGATCTTGTAGACGGCGACTTCGCGAACCACCAGTAGCGTGTGCTCGAAACCCTCTTCCTCCTCCTCTAACGACATCGTTTTGGTTGCTTTGGAGTTTGAATTTGAGTTTATTGTGAATGAATTTAAGAGACGCGTTTGTTGTTGTTGTTTAATTTGGAAATTGAAGATTGGTGTGGTGTGGGGTGTGGTGTCTTTCTCAACTCTGTCTCAAAGAAAACACCAAGGTCAACTGTTTTGGGAGATAGATTAGAAATACACACAATTATCACATTACCAATATGCTGCTCTTTGTTCCTCCATTTTCTTACCTTTCATACCTTACTTCTAAATGATTTTCCTTTCAGACCCCCGCGTACTCTGCGTTGAT
TARGET=1335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00581 Ps_contig_mira-and-tgicl-ass_franssen_5548
SEQUENCE=TTTCAAAAGCAGTGCTCAGTTTGGCGTTGTTTTTTTGTAATTGTTTTCAAAGGCGCTGACTCTTCCTTCTGCTTGCAAAGTCTCTGCTCCCTCTCTCTCTAACTGTGGATTGTCTGTAACCCCTGCTGCTGCTCCTGGTGTAAGTCTCTCTCCAGCATCTTCTCCTACTGAAGCACCAGGTGTGGCCACTCCTGTGCTTCCATCTAGAGTACCGGCAGCCGCGCCGGGCGTCAAGAGTGCGGCAGCATCAGCATTGCTCCCTATTTCAGCAGGATCCATTCTTGTTTGTCTCTTATTTCCATTCTCAAGCATTTGAGTAGCCATGTTTAGTTTCATTGAGAGTAGGAAGGTTATGTCATGAGGGATTATTTTGAGTTTTGAAGGTTTTTTTTGGAGTATTATTACCATTAGAGCTATTTTCTTTGTGTGTGCAATTTAAACCTATTCATGTATATTTATTTATTTATGAGTAGTGTAATCTTGCTATTTTTTATTTTGTTGTACG
TARGET=91,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2546
PRIMER_LEFT_PENALTY=0.185055
PRIMER_RIGHT_PENALTY=0.069526
PRIMER_LEFT_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_SEQUENCE=GAATGGATCCTGCTGAAATAGG
PRIMER_LEFT=61,22
PRIMER_RIGHT=280,22
PRIMER_LEFT_TM=60.185
PRIMER_RIGHT_TM=59.930
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.3362
PRIMER_LEFT_1_PENALTY=0.185055
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_1_SEQUENCE=CCGGTACTCTAGATGGAAGCAC
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.4598
PRIMER_LEFT_2_PENALTY=0.185055
PRIMER_RIGHT_2_PENALTY=0.274768
PRIMER_LEFT_2_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_2_SEQUENCE=CTGCCGGTACTCTAGATGGAAG
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=217,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=60.275
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.4627
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.277599
PRIMER_LEFT_3_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_3_SEQUENCE=AGGAGAAGATGCTGGAGAGAGA
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.722
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.4870
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_4_SEQUENCE=ACCTGGTGCTTCAGTAGGAGAA
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_00582 Ps_contig_mira-and-tgicl-ass_franssen_5548
SEQUENCE=TTTCAAAAGCAGTGCTCAGTTTGGCGTTGTTTTTTTGTAATTGTTTTCAAAGGCGCTGACTCTTCCTTCTGCTTGCAAAGTCTCTGCTCCCTCTCTCTCTAACTGTGGATTGTCTGTAACCCCTGCTGCTGCTCCTGGTGTAAGTCTCTCTCCAGCATCTTCTCCTACTGAAGCACCAGGTGTGGCCACTCCTGTGCTTCCATCTAGAGTACCGGCAGCCGCGCCGGGCGTCAAGAGTGCGGCAGCATCAGCATTGCTCCCTATTTCAGCAGGATCCATTCTTGTTTGTCTCTTATTTCCATTCTCAAGCATTTGAGTAGCCATGTTTAGTTTCATTGAGAGTAGGAAGGTTATGTCATGAGGGATTATTTTGAGTTTTGAAGGTTTTTTTTGGAGTATTATTACCATTAGAGCTATTTTCTTTGTGTGTGCAATTTAAACCTATTCATGTATATTTATTTATTTATGAGTAGTGTAATCTTGCTATTTTTTATTTTGTTGTACG
TARGET=453,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00583 Ps_contig_mira-and-tgicl-ass_franssen_5573
SEQUENCE=ATCCCAAACCCCAACTTTAGTCTCATGAGGTTAGGATATCTTCTACATTAATGAACAACTGCTCCCAGACTCCTTGGCAATAGCTTTTGCAAGCATGGTTTTCCCAGTGCCAGGGGGTCCATACAACAGAACCCCCTTCTGTGGTCCAAGAAGCTTGCCATGCGAAAACAAGTCGGGCCGTTTCAAGGGTAGAATAACAAGCTCAAACAAAGCTTGTTTAATAGTGTCCAGTCCCCCAATGGAGTCAAATTCCACATCAATATGGTCAGGGTTTATAACATCACAGGCTATTACATCCTCATAGGGATTGGTTTGAATGAGGGGACGACCTAGACGCTTGGCAATCTGTTTCTTTTGTTCAAGAGCTTTCTTGGTTGATTCACGGTTGGGGTCAAGGTATCGGAGTCCGGTGACCAATACCCAGTAACTCAGCGCCGCCCTAGCTGCGTACAACAACAGCTCTTGCAGAATCTTGGTATTGGCTGAACCGGTCATTCTCTAAAGTTCAAAGTATAACCCTAAAACTTAGGGTAAAAAAATTATCTTTTCTTCTTCTTCGAAGTATAGCTCCTAAAATTCAAAAGTATTATCAGCTCTCTGTGAGAGAGACCACCGAGATTTTAGGATGAAATGAAATACGAGGTCGTGAAATAAAAGGAAAAATCGAMG
TARGET=547,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00584 Ps_contig_mira-and-tgicl-ass_franssen_5575
SEQUENCE=AATTTGGACATGATCTGACACTGAATGAAGCAAGCAAGCGATTCCAGGCTTACTTAGAAGACAGAAATACTCCACTTCTTCCACCTGATATAAGAAGGGCAGTTTATGTGGCTGTAGTAAAACAGGCAACCAAATCTATCCGGTCGGGTTATGAATCACTTCTGAAAGTATACAGAGAAACTGACCTAAGTGAGGAGAAAACACGTATTCTGGGCTCACTGTCTTCTTCATCTGATCCAGACCTAATTCTTGAAGTTTA
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9764
PRIMER_LEFT_PENALTY=0.861164
PRIMER_RIGHT_PENALTY=0.115189
PRIMER_LEFT_SEQUENCE=TTGGACATGATCTGACACTGAA
PRIMER_RIGHT_SEQUENCE=AGATGAAGAAGACAGTGAGCCC
PRIMER_LEFT=3,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=59.139
PRIMER_RIGHT_TM=59.885
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.9764
PRIMER_LEFT_1_PENALTY=0.861164
PRIMER_RIGHT_1_PENALTY=0.115189
PRIMER_LEFT_1_SEQUENCE=TTTGGACATGATCTGACACTGA
PRIMER_RIGHT_1_SEQUENCE=AGATGAAGAAGACAGTGAGCCC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.139
PRIMER_RIGHT_1_TM=59.885
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=1.1265
PRIMER_LEFT_2_PENALTY=1.011297
PRIMER_RIGHT_2_PENALTY=0.115189
PRIMER_LEFT_2_SEQUENCE=GGACATGATCTGACACTGAATGA
PRIMER_RIGHT_2_SEQUENCE=AGATGAAGAAGACAGTGAGCCC
PRIMER_LEFT_2=5,23
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=59.885
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=1.2922
PRIMER_LEFT_3_PENALTY=0.861164
PRIMER_RIGHT_3_PENALTY=0.431057
PRIMER_LEFT_3_SEQUENCE=TTGGACATGATCTGACACTGAA
PRIMER_RIGHT_3_SEQUENCE=TCAGAAGTGATTCATAACCCGA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=59.139
PRIMER_RIGHT_3_TM=59.569
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=1.2922
PRIMER_LEFT_4_PENALTY=0.861164
PRIMER_RIGHT_4_PENALTY=0.431057
PRIMER_LEFT_4_SEQUENCE=TTTGGACATGATCTGACACTGA
PRIMER_RIGHT_4_SEQUENCE=TCAGAAGTGATTCATAACCCGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=59.139
PRIMER_RIGHT_4_TM=59.569
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_00585 Ps_contig_mira-and-tgicl-ass_franssen_5628
SEQUENCE=GATTGTTAACAATGTTGGGTCTTTCASAAGGTACATATCATCATTACATTCTTTGCCTTCTCACACATTCTCTCTCTCTCTCTCTCACMTTCTTTCTCTTCTGTCTACTTTTMGAACACAGCAACAAACTAAAATWACCAAAACCTTAGTTTACGGTTTAATCCGCTT
TARGET=69,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00586 Ps_contig_mira-and-tgicl-ass_franssen_5630
SEQUENCE=CCGTTCTSACACATTCTCTCTCTCTCTCTCTCCAACTCTTTTCTCTTCTGTCACTTTTGAACTACTAGCACAACAAAAATTACAAAACCTATTCGGTTTAATCGCTCTTATT
TARGET=15,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00587 Ps_contig_mira-and-tgicl-ass_franssen_5642
SEQUENCE=TCACMAAAACTTACTCAAATCGATGAAGAACAATCACTCTCTCCGGATTCTGTTATCAACACTTGGGAACTCATGGATGGTTTGGATGAACATGAAGATTCTAATGTTCATAAAGCTTTAATCTTTGATAACCCAGTGAGTTTTTCAGATAAACACAGTTCATGCAGGTACACAGCCTTTGATGGGTCTGCAAAAAAGAAACTTCTTGATTCATTTGAATCACTGAAAGCTTCGGAAGCTGTAATGGAAGAAAAAAATTCCAAATCTTTTGTGAAGAAACCACTTTGGAAGCATTTATCAGAAGAAGCTTTGTTAGCTAAGTTGGATCCAAGTGTTGCTTGGAGTTACAGAAGAGCATTATCTTCAAGACAACTAGGTTGCAACAACAACAAAAACCGGTTAAGATGTGTAAGATCAATGGAATCAAGTCCTATGAACCCTTCTTGTTCTTCTTTGTTTGGTAAAAGTTTGTGTCTTTTACCAGGAACAGAAGACAGAATTGTTGTTTACTGCACAAGTTTGCGAGGGATTCGAAAGACTTACGAGGATTGTTGTTCAGTGAGGATGATTCTGAGAGGATTTCGAGTTGCGGTCGATGAAAGAGACATTTCGATGGATTCATCTTACAGAAAGGAGTTACAGAATGCACTTGGTGGAAAATCAGTAGTGACATTGCCACAGGTTTTTATCAGAGGGAAACATGTTGGAAATGCGGAGGATTTGAAACAATTGAATGAATCCGGTGAATTGGCGAAACTGTTGAGTGGTTTTCCAACTCAAGATCTTTGGTTTGTTTGTGATAAATGTGGTGATGCAAGGTTTGTGCCTTGTGATAATTGCAATGGTAGCAGGAAAGTGTTTGAGGAAGAACAAGGGAAGTTGAAAAGGTGTGTTCATTGTAATGAGAATGGATTGATAAGATGCACAAGCTGTTGTTCATGAATCAGTGATTACCAAAATTACTGATTGATTGATGTTATCTTTTTATTGTTGTTTTTGTTTTTCTTGGGTTGAGTTTGTTATTCTGATAAATTTGGCAGCTACTATTAATGAATATGCATGTTTCCATTTAGATTCGC
TARGET=381,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00588 Ps_contig_mira-and-tgicl-ass_franssen_5642
SEQUENCE=TCACMAAAACTTACTCAAATCGATGAAGAACAATCACTCTCTCCGGATTCTGTTATCAACACTTGGGAACTCATGGATGGTTTGGATGAACATGAAGATTCTAATGTTCATAAAGCTTTAATCTTTGATAACCCAGTGAGTTTTTCAGATAAACACAGTTCATGCAGGTACACAGCCTTTGATGGGTCTGCAAAAAAGAAACTTCTTGATTCATTTGAATCACTGAAAGCTTCGGAAGCTGTAATGGAAGAAAAAAATTCCAAATCTTTTGTGAAGAAACCACTTTGGAAGCATTTATCAGAAGAAGCTTTGTTAGCTAAGTTGGATCCAAGTGTTGCTTGGAGTTACAGAAGAGCATTATCTTCAAGACAACTAGGTTGCAACAACAACAAAAACCGGTTAAGATGTGTAAGATCAATGGAATCAAGTCCTATGAACCCTTCTTGTTCTTCTTTGTTTGGTAAAAGTTTGTGTCTTTTACCAGGAACAGAAGACAGAATTGTTGTTTACTGCACAAGTTTGCGAGGGATTCGAAAGACTTACGAGGATTGTTGTTCAGTGAGGATGATTCTGAGAGGATTTCGAGTTGCGGTCGATGAAAGAGACATTTCGATGGATTCATCTTACAGAAAGGAGTTACAGAATGCACTTGGTGGAAAATCAGTAGTGACATTGCCACAGGTTTTTATCAGAGGGAAACATGTTGGAAATGCGGAGGATTTGAAACAATTGAATGAATCCGGTGAATTGGCGAAACTGTTGAGTGGTTTTCCAACTCAAGATCTTTGGTTTGTTTGTGATAAATGTGGTGATGCAAGGTTTGTGCCTTGTGATAATTGCAATGGTAGCAGGAAAGTGTTTGAGGAAGAACAAGGGAAGTTGAAAAGGTGTGTTCATTGTAATGAGAATGGATTGATAAGATGCACAAGCTGTTGTTCATGAATCAGTGATTACCAAAATTACTGATTGATTGATGTTATCTTTTTATTGTTGTTTTTGTTTTTCTTGGGTTGAGTTTGTTATTCTGATAAATTTGGCAGCTACTATTAATGAATATGCATGTTTCCATTTAGATTCGC
TARGET=964,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00589 Ps_contig_mira-and-tgicl-ass_franssen_5679
SEQUENCE=CGTTTATCCTTCCTTTTCAACCAATAAGGTGAARATGACRTCGGCTCTTTCATCTTATGCTAAGTATTTCAATTACAGTAGTTTTTCTAGTTGTCGTAATCATTCAGTAATGCATTTATAATCCCTTATTCCTTTAATATGTAGTTATATTTTTGACAGTGCTATAGTTTTGACAATGTTATAGTTCTTCTGTTAGTTAGAATTACATCAGCATGGGTGATTCAGTTTGTTTAAAAATTTTGTTTCTGTATTACTCTAATTTCATATTAATATTCTGGAGTGGAGGCATGTTAWTTTTTAAATTTCTTGTTTGCATAATTATCTATCATTATTATTATTATTTGTGGAGTTAGTTGTACTATCGGTTCTTTGATAACACTCATTCCTACTCTTGTATGTTTATTAAATTAATTATTTTGTAGT
TARGET=328,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00590 Ps_contig_mira-and-tgicl-ass_franssen_5697
SEQUENCE=CAATCCTGCAACTTGATTGTTTGAGCTGGACTCAGACACTGCCTTCGTTACGAATTTGTCGAATTTTACGTTACATGATGGTGAAACCTGCTGGAAGGGTTTCACCGATGCAACATGACAAACACTTCCTAGTTTATCACATGAACCAACCTTAACCCTGCCTCCAAGAATCACAGAACCTAACTTCTGTGATGAAGTAACCCTAGGTCTTGACATTGCAACCGAAATATTAGAAACCGATGTTGTTGTAGCCATTTCGAATTATCCTAATCTGAACCTAAATCTTTTGGCAACGAAGGGATGAATGTGGAGAGAGAGAAATGAGAGAGTTTGAAACGGTATGTATGAGATGAAACTGAGGGTAGAAAATGGAGGAGTGGAATAAACGAAAAACCTTGGAAGTGTGTGTCGATGGAAACAATGTTGTTGTGTTTTAATGTGAAGTTGTTTAGAGTCC
TARGET=310,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0605
PRIMER_LEFT_PENALTY=0.003602
PRIMER_RIGHT_PENALTY=0.056850
PRIMER_LEFT_SEQUENCE=GAACCTAAATCTTTTGGCAACG
PRIMER_RIGHT_SEQUENCE=ACACACTTCCAAGGTTTTTCGT
PRIMER_LEFT=273,22
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.943
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.1682
PRIMER_LEFT_1_PENALTY=0.003602
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=GAACCTAAATCTTTTGGCAACG
PRIMER_RIGHT_1_SEQUENCE=TCGTTTATTCCACTCCTCCATT
PRIMER_LEFT_1=273,22
PRIMER_RIGHT_1=388,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.1682
PRIMER_LEFT_2_PENALTY=0.003602
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=GAACCTAAATCTTTTGGCAACG
PRIMER_RIGHT_2_SEQUENCE=TTCGTTTATTCCACTCCTCCAT
PRIMER_LEFT_2=273,22
PRIMER_RIGHT_2=389,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.1781
PRIMER_LEFT_3_PENALTY=0.121299
PRIMER_RIGHT_3_PENALTY=0.056850
PRIMER_LEFT_3_SEQUENCE=CTAAATCTTTTGGCAACGAAGG
PRIMER_RIGHT_3_SEQUENCE=ACACACTTCCAAGGTTTTTCGT
PRIMER_LEFT_3=277,22
PRIMER_RIGHT_3=406,22
PRIMER_LEFT_3_TM=60.121
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.1859
PRIMER_LEFT_4_PENALTY=0.129072
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=GCAACCGAAATATTAGAAACCG
PRIMER_RIGHT_4_SEQUENCE=ACACACTTCCAAGGTTTTTCGT
PRIMER_LEFT_4=217,22
PRIMER_RIGHT_4=406,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_00591 Ps_contig_mira-and-tgicl-ass_franssen_5703
SEQUENCE=GGCATTTGTAAGGCGGTGCAATCTACAAAAATAGCTTTCTGGGGTGAAATTCATTCCCCGTGACCAGGTAGAAGATCGGGACTTAGATAAGGATTCTGTCTCCAAGGAAAGAAAGAAATCAGACAGTAGGAGGAAAGAGGATAAGAGCCCTAGGAATAGCTCTTCTGATGAGGAGGAGCTTGATAGAATAAAAGGTTCTAGAAAGAAGAAGTTGTATTCATCGGATGAAGATTCTTCATTTTATACAATCGACAGTGAAAGTGATCGGGATGATAAGAAAAGGAAAAGTAAAGGAAAGAAGAAAAAGGACAAGAGTTATAGGAATATCTCTTCTGATGAGGATGCGTTGGATGAGATGAAAAAGGGATCTAGAAAGAAGCGGTACTCATCTGATGAGAATTCTTCATTTTATACAAGCGAAAGTGAAAGCGACCGGGATGATAAGAAAAGGAGAAGGAAAGGAAAGAAGAAAAAGGGTAGGAGTAGAGAGAACAAGATCAAAGGAGAAGCTGCCGACATTGCAGCCAATTCTACAAGTAAGAAGGATATTGCTAGAAAAGAAATGGGGTTGGATTGG
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0793
PRIMER_LEFT_PENALTY=0.390220
PRIMER_RIGHT_PENALTY=0.689105
PRIMER_LEFT_SEQUENCE=CCAGGTAGAAGATCGGGACTTA
PRIMER_RIGHT_SEQUENCE=CGATCACTTTCACTGTCGATTG
PRIMER_LEFT=63,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=59.610
PRIMER_RIGHT_TM=60.689
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=1.0966
PRIMER_LEFT_1_PENALTY=0.460533
PRIMER_RIGHT_1_PENALTY=0.636023
PRIMER_LEFT_1_SEQUENCE=ACAAAAATAGCTTTCTGGGGTG
PRIMER_RIGHT_1_SEQUENCE=GGCTCTTATCCTCTTTCCTCCT
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=148,22
PRIMER_LEFT_1_TM=59.539
PRIMER_RIGHT_1_TM=59.364
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=1.0966
PRIMER_LEFT_2_PENALTY=0.460533
PRIMER_RIGHT_2_PENALTY=0.636023
PRIMER_LEFT_2_SEQUENCE=ACAAAAATAGCTTTCTGGGGTG
PRIMER_RIGHT_2_SEQUENCE=AGGGCTCTTATCCTCTTTCCTC
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=59.539
PRIMER_RIGHT_2_TM=59.364
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=1.0982
PRIMER_LEFT_3_PENALTY=0.462203
PRIMER_RIGHT_3_PENALTY=0.636023
PRIMER_LEFT_3_SEQUENCE=CAAAAATAGCTTTCTGGGGTGA
PRIMER_RIGHT_3_SEQUENCE=GGCTCTTATCCTCTTTCCTCCT
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=148,22
PRIMER_LEFT_3_TM=60.462
PRIMER_RIGHT_3_TM=59.364
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=1.0982
PRIMER_LEFT_4_PENALTY=0.462203
PRIMER_RIGHT_4_PENALTY=0.636023
PRIMER_LEFT_4_SEQUENCE=CAAAAATAGCTTTCTGGGGTGA
PRIMER_RIGHT_4_SEQUENCE=AGGGCTCTTATCCTCTTTCCTC
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=60.462
PRIMER_RIGHT_4_TM=59.364
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00592 Ps_contig_mira-and-tgicl-ass_franssen_5705
SEQUENCE=ATAAGGATTCTGTCTCCAAGGAAAGAAAGAAATCAGACAGTAGGAGGAAARAGGATAGGAGCCCTAGGAATAGCTCTTCTGATGAGCAGGAGCTTGATAGAATAAAAGGTTCTAGAAAGAAGAAGTGGTATTCATCGGATGAAGATTCTTCATTTTACAGTGAAAGTGATCGGGATGATAAGAAAAGGAAAAGGAAAGGAAAGAAAGAAAAAGGGACAAGAGTTATAGGAATATCTCTTCTGATGAGGATGCGTTGGATGAGATGAAAAAGGGATCTAGAAAGAAGCGGTACTCATCTGATGAGAATTCTTCTATTTTATACAAGCGAAAGTGAAAGCGACCGGGATGAATAAAGAAAAAGGAAAAGGAAAAAAAAACGGG
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00593 Ps_contig_mira-and-tgicl-ass_franssen_5705
SEQUENCE=ATAAGGATTCTGTCTCCAAGGAAAGAAAGAAATCAGACAGTAGGAGGAAARAGGATAGGAGCCCTAGGAATAGCTCTTCTGATGAGCAGGAGCTTGATAGAATAAAAGGTTCTAGAAAGAAGAAGTGGTATTCATCGGATGAAGATTCTTCATTTTACAGTGAAAGTGATCGGGATGATAAGAAAAGGAAAAGGAAAGGAAAGAAAGAAAAAGGGACAAGAGTTATAGGAATATCTCTTCTGATGAGGATGCGTTGGATGAGATGAAAAAGGGATCTAGAAAGAAGCGGTACTCATCTGATGAGAATTCTTCTATTTTATACAAGCGAAAGTGAAAGCGACCGGGATGAATAAAGAAAAAGGAAAAGGAAAAAAAAACGGG
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00594 Ps_contig_mira-and-tgicl-ass_franssen_5706
SEQUENCE=TATGTGTTTCTCTTCACAACATGACACCTTTTACTTGCTCCCTACCTCTTCCAGAAACATAGGAACCTTAGATTAACTCACCNCAAAGAAGGAGAACACAATTGAAAGAAGCAAAGAAAGGAAAAAGATGATTATAGTGAAAGAAGAGATTAAGATGAAGAACTTGAAATTGTATATGGAGAATCAAACCATCATTGAGGAGAATGAAAAGTTGAGGAAACAAGCTATGCTTTTGCATAAAGAGAATCAAGACCTTATGTCTCAGCTTCAAATTAAGCTTTCACTACAAAATATCAATACCAACAATAACTAGTTTTTCAATTAATTAATTTTCTAGCAATAAGTTTAGAGTTATATAATAAAGATATTGAATAGGTAATATTTTAATTGTAACATTGCATAGAATATGATATTGTGCAATATTGTTGAAATGGGTCAAATGTTTGACTTCTTGATGGTATGTTTAGAGAAGGGAAAATAGAAAGCAAAAATGTCTTGGTTTTACTTATATTATGAGTGTAATTGTATTAAAGTCAAGCAAGTAGTTAAAAATTAAAAAGTTTTTTTTTG
TARGET=320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5826
PRIMER_LEFT_PENALTY=1.496015
PRIMER_RIGHT_PENALTY=3.086550
PRIMER_LEFT_SEQUENCE=GAATCAAACCATCATTGAGGAG
PRIMER_RIGHT_SEQUENCE=TCCCTTCTCTAAACATACCATCAAG
PRIMER_LEFT=180,22
PRIMER_RIGHT=474,25
PRIMER_LEFT_TM=58.504
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=4.5826
PRIMER_LEFT_1_PENALTY=1.496015
PRIMER_RIGHT_1_PENALTY=3.086550
PRIMER_LEFT_1_SEQUENCE=GGAGAATCAAACCATCATTGAG
PRIMER_RIGHT_1_SEQUENCE=TCCCTTCTCTAAACATACCATCAAG
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=474,25
PRIMER_LEFT_1_TM=58.504
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=4.5826
PRIMER_LEFT_2_PENALTY=1.496015
PRIMER_RIGHT_2_PENALTY=3.086550
PRIMER_LEFT_2_SEQUENCE=GAATCAAACCATCATTGAGGAG
PRIMER_RIGHT_2_SEQUENCE=CCCTTCTCTAAACATACCATCAAGA
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=473,25
PRIMER_LEFT_2_TM=58.504
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=4.5826
PRIMER_LEFT_3_PENALTY=1.496015
PRIMER_RIGHT_3_PENALTY=3.086550
PRIMER_LEFT_3_SEQUENCE=GGAGAATCAAACCATCATTGAG
PRIMER_RIGHT_3_SEQUENCE=CCCTTCTCTAAACATACCATCAAGA
PRIMER_LEFT_3=177,22
PRIMER_RIGHT_3=473,25
PRIMER_LEFT_3_TM=58.504
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=4.6512
PRIMER_LEFT_4_PENALTY=1.564634
PRIMER_RIGHT_4_PENALTY=3.086550
PRIMER_LEFT_4_SEQUENCE=TGAGGAGAATGAAAAGTTGAGG
PRIMER_RIGHT_4_SEQUENCE=TCCCTTCTCTAAACATACCATCAAG
PRIMER_LEFT_4=195,22
PRIMER_RIGHT_4=474,25
PRIMER_LEFT_4_TM=58.435
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_00595 Ps_contig_mira-and-tgicl-ass_franssen_5738
SEQUENCE=TCCTCCTAGATCTCTCCTCATCACTTCCTTCAATGGCTACTTCTTCTTCTTCTTCTTCACCTATTAGAAGATTCTATCTTATTCTTTACAATTGGACTGTTTTATTTGG
TARGET=40,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00596 Ps_contig_mira-and-tgicl-ass_franssen_5741
SEQUENCE=CKTAGCAAAAACTTTAGGATATATCTTTCAAGCCACACTTACATAYATAGTAGAGGAAATTTTTTATTAATTGAAAGAAATGAACAGCTCAGTTGCAATTACAAATATCGCGTCTATAGTCAGGAAACCATATACTGCTTAACTATTAGTATTATTTAGGTCCAAATAAACGATTCCCTGTCGTATATATATAGCTCTCTCTTTTTACATACTAGTATGTTGTAGTTGGTGGAAGCTAGATGAATCCCCAAAATGGAGAACCTTTTTGTACTGAATCCACGGCTTGACCACCACGACGGAGGTGAACATTCTGCGAACACCATTATGATCAACGGCTGAAATATTGAGATAATACTTCATCCCTGACACCACTTGCTTCTTTGCCTCCACCACCTCCACAAACTTCAACGCCTCGCCTCCGCCGTTGCTTTTTTTGTAGTTGTACTCCTCTACCGCGAACTTCCCAAGCTCCTGCACCTCCATGTTTGTCCTCACGTCAGTAATCTCCGTCTTCGCTCCAACTATCAGCCGGCCACACGACGCCGTTGATAGAATGCAGAGCAAGCTGGTTATGAGGATTGCAATATTCACAGGAACAGCCATCATAAAACCACAAAAGAAATGATTAACTTGTGTGTAGGGTAGGGTGTATAAGAAGGAGAATGAATGAAAAGATATCCCCGACTCTTGCGTTGGATACCACTGCTT
TARGET=184,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00597 Ps_contig_mira-and-tgicl-ass_franssen_5742
SEQUENCE=CGGGGGTTTATTTAATTTTTTAAAGTTAGGAGGCACACACACCAGAAAGGTCAACGATCGAAAGAGGAGGCGGTGCCACTGGCAAACTGACAGAACTCGAATCATGATAGGCATATCAGTTACCGGTATCATGTACACACTCTCCCCAACAATCTCTTCATTCACGCGCTTCTCACCCACTTTTTTCACTTCCAACTTTCGTCTTCGAGCTTCTTCTTCTTACAATACTTCTTCAACCACATTTCTTGACACCAATCCAACACTGGATTCCGTTGTAG
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1715
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=CACTGGCAAACTGACAGAACTC
PRIMER_RIGHT_SEQUENCE=GTGTTGGATTGGTGTCAAGAAA
PRIMER_LEFT=76,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.1715
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=CACTGGCAAACTGACAGAACTC
PRIMER_RIGHT_1_SEQUENCE=GGTGTCAAGAAATGTGGTTGAA
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.1845
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=CTCCCCAACAATCTCTTCATTC
PRIMER_RIGHT_2_SEQUENCE=GTGTTGGATTGGTGTCAAGAAA
PRIMER_LEFT_2=141,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=0.1845
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=TCTCCCCAACAATCTCTTCATT
PRIMER_RIGHT_3_SEQUENCE=GTGTTGGATTGGTGTCAAGAAA
PRIMER_LEFT_3=140,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.1845
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=CTCCCCAACAATCTCTTCATTC
PRIMER_RIGHT_4_SEQUENCE=GGTGTCAAGAAATGTGGTTGAA
PRIMER_LEFT_4=141,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=112
=
PRIMER_SEQUENCE_ID=SSR_00598 Ps_contig_mira-and-tgicl-ass_franssen_5745
SEQUENCE=GGACCCAAATATCAATATCATAACAAAATCTCTCTCTCGAATTACAGAACAAGTGTTGTGAGGGAACAGTGGATGGGGTGCTGCTAGTTTCTCACTTCATTCCAAAAATGGTGCACAGACTCTCTCTAATGGCTTCTCACGTCTACCCTTCACCCTTGCCCTTCCCCACATCACACATCAAGGACTGTCAAACTTTTGCACCTTCTTTCTTTTGACAAAAACCAGACATGCTCAATTATCAGTACTTCCTTTTACGTTAAGGGCGAGTCCATTTGTAGAGTCGATGAAAACCCAAAATAAATGGTTTGATGAAAGT
TARGET=29,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00599 Ps_contig_mira-and-tgicl-ass_franssen_5752
SEQUENCE=CGAACAANACAGTTNAGAGAGWACATGATCGGTGTTCCTAACGGTGCCCSTTTGGGTTGCCGGTAGGGGTTGGCAGCGGATGAACAGTAGATGAGGTAAGCCAAGACTTCTCTGTCTTCGGTTGAGAGAGCAGCTGCTAGGGTGAGGATGGTTGCCGGAAGGAAGGAAAGTTGGTCGGTGATAACCGGTGGGGTTGGATGAACCGTCGCTTTATGGTAGAGCTTCTTCATGGTTGAACAAAAAGATGAAAAAAGTC
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00600 Ps_contig_mira-and-tgicl-ass_franssen_5756
SEQUENCE=TTAAAAATAAAACCAAGTTCATAATACTCCCAACCTAAAAAGTAAAAAGTTACATTCATAAATCATCATAATTATACACTAGAGTACTTCTAAAGTATATCAAGATTTTCCAGATGCTCAATTTTTTCATGCATGAAACTGTAACCGTCCTCCGCAACGAGTCTTGACAACCCGGCCACAGAATATCAAAGAAATAAATAAATCACCAAAAATTTACTAGGACAGTCTTCATGCATCTTCTGTTTTTTTATCTGATCTTCCAATTGACACTTATCACTGAACTAGTTTCCTGCTTCACCTTCCAAGTTTGAACCTTTTTGTGG
TARGET=192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8899
PRIMER_LEFT_PENALTY=0.428205
PRIMER_RIGHT_PENALTY=0.461713
PRIMER_LEFT_SEQUENCE=CATGCATGAAACTGTAACCGTC
PRIMER_RIGHT_SEQUENCE=AAAGGTTCAAACTTGGAAGGTG
PRIMER_LEFT=127,22
PRIMER_RIGHT=316,22
PRIMER_LEFT_TM=60.428
PRIMER_RIGHT_TM=59.538
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=1.1791
PRIMER_LEFT_1_PENALTY=0.428205
PRIMER_RIGHT_1_PENALTY=0.750914
PRIMER_LEFT_1_SEQUENCE=CATGCATGAAACTGTAACCGTC
PRIMER_RIGHT_1_SEQUENCE=CCACAAAAAGGTTCAAACTTGG
PRIMER_LEFT_1=127,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=60.428
PRIMER_RIGHT_1_TM=60.751
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=1.2344
PRIMER_LEFT_2_PENALTY=0.772692
PRIMER_RIGHT_2_PENALTY=0.461713
PRIMER_LEFT_2_SEQUENCE=CATGAAACTGTAACCGTCCTCC
PRIMER_RIGHT_2_SEQUENCE=AAAGGTTCAAACTTGGAAGGTG
PRIMER_LEFT_2=131,22
PRIMER_RIGHT_2=316,22
PRIMER_LEFT_2_TM=60.773
PRIMER_RIGHT_2_TM=59.538
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=1.3753
PRIMER_LEFT_3_PENALTY=0.913575
PRIMER_RIGHT_3_PENALTY=0.461713
PRIMER_LEFT_3_SEQUENCE=GCATGAAACTGTAACCGTCCTC
PRIMER_RIGHT_3_SEQUENCE=AAAGGTTCAAACTTGGAAGGTG
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=316,22
PRIMER_LEFT_3_TM=60.914
PRIMER_RIGHT_3_TM=59.538
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=1.5236
PRIMER_LEFT_4_PENALTY=0.772692
PRIMER_RIGHT_4_PENALTY=0.750914
PRIMER_LEFT_4_SEQUENCE=CATGAAACTGTAACCGTCCTCC
PRIMER_RIGHT_4_SEQUENCE=CCACAAAAAGGTTCAAACTTGG
PRIMER_LEFT_4=131,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=60.773
PRIMER_RIGHT_4_TM=60.751
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_00601 Ps_contig_mira-and-tgicl-ass_franssen_5784
SEQUENCE=AAAGACAAGTAGGTAATCAACGCAGAGTTTAGGTTGTTTCATGGAGCAAATGAGGTCTTGCTCACTGAAGGCTCAAGTGGTAGATCAATATCATCAAAGCTGTTCTAAAATGAAAAATAAATAAATTTGTTAGTCCACTACTGGTTTCTACTCAGTGCTCATCAATGTATACATCACTAAAAACATGTGAGAAATTGAGTACTTTCTTTGTTGTATCTAAATTTTCCCTTTTAACTACTATTTATTTTTACTCAACCTTAGTAAAGGTTTTAAACAAAGGACAAAGATCTTGTATTGTTCTACAAAGGATCATATATGAACTGT
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4573
PRIMER_LEFT_PENALTY=0.385416
PRIMER_RIGHT_PENALTY=1.071885
PRIMER_LEFT_SEQUENCE=GTTGTTTCATGGAGCAAATGAG
PRIMER_RIGHT_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT=32,22
PRIMER_RIGHT=163,23
PRIMER_LEFT_TM=59.615
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=1.5336
PRIMER_LEFT_1_PENALTY=0.461740
PRIMER_RIGHT_1_PENALTY=1.071885
PRIMER_LEFT_1_SEQUENCE=CACTGAAGGCTCAAGTGGTAGA
PRIMER_RIGHT_1_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=163,23
PRIMER_LEFT_1_TM=59.538
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=1.5336
PRIMER_LEFT_2_PENALTY=0.461740
PRIMER_RIGHT_2_PENALTY=1.071885
PRIMER_LEFT_2_SEQUENCE=TCACTGAAGGCTCAAGTGGTAG
PRIMER_RIGHT_2_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=163,23
PRIMER_LEFT_2_TM=59.538
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=1.5336
PRIMER_LEFT_3_PENALTY=0.461740
PRIMER_RIGHT_3_PENALTY=1.071885
PRIMER_LEFT_3_SEQUENCE=CTCACTGAAGGCTCAAGTGGTA
PRIMER_RIGHT_3_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=163,23
PRIMER_LEFT_3_TM=59.538
PRIMER_RIGHT_3_TM=59.928
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=1.8886
PRIMER_LEFT_4_PENALTY=0.816680
PRIMER_RIGHT_4_PENALTY=1.071885
PRIMER_LEFT_4_SEQUENCE=ATGAGGTCTTGCTCACTGAAGG
PRIMER_RIGHT_4_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=163,23
PRIMER_LEFT_4_TM=60.817
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_00602 Ps_contig_mira-and-tgicl-ass_franssen_5792
SEQUENCE=TTGTCGCCGGAGAACCATTTCCGGTGGATGTTTCAGAAACGACGACTGTGTACGACGGTGGTTGCGGCGGGAAGAAAGCGGACTTAGCAGCAATCGAGGACGTTACTCCGCCCATGGTTGCTTTGATTCATAAAGACGGCAGTGACGGCGGTTGCGGCGGTGAGATTTCAGATTCAGAAAGAAAGAAGAGCGGCGCGTGTAAGTTTTTTGTTTGTGGTTGGTGTAAATTAATTTCTTTAAGTTTTTTTC
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00603 Ps_contig_mira-and-tgicl-ass_franssen_5843
SEQUENCE=AATWKTAATAATAATAATAATAATGTAATAATTAACAACCAAAGTGTAGCAACCCATCAACATGTATTTATAGTTGACCCATCTAGAATTAGGAGAAACATTTTCCTAATAATAACAAATTAATGATAAGGACATAATATATTCCATTTTCGTCCTCAATCTTAACGACACGTCCTCACATTAATTCTCAATCCAAAAAATATCAATAAAAATCATTTCCCTACATTATTTATAGATGAAGACTTCCATCAAATATTTAATTAATGTAAGATATTCCATCGCGTATCAAGATTGGGGGACGAAAATGGTACTGCAAATTTACTACTAGGTAC
TARGET=6,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00604 Ps_contig_mira-and-tgicl-ass_franssen_5855
SEQUENCE=ACAATAACACCCTACAAAAAGTAACTTGATCCTAATTTGTTATCTTCTGTATAGTTCAACATCTGCACAATTAAATTTAAAACAACATGCATGAAACAAATAAATAAATAAAAAAAATTATAACTGTAACCTAGGTAAACAAAAGATTGACAAATACAACTCAACAACAGGTGGAGT
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.9671
PRIMER_LEFT_PENALTY=3.592105
PRIMER_RIGHT_PENALTY=1.375005
PRIMER_LEFT_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_SEQUENCE=ACTCCACCTGTTGTTGAGTTGT
PRIMER_LEFT=7,25
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=59.408
PRIMER_RIGHT_TM=58.625
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=5.8022
PRIMER_LEFT_1_PENALTY=3.592105
PRIMER_RIGHT_1_PENALTY=2.210129
PRIMER_LEFT_1_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_1_SEQUENCE=CTCCACCTGTTGTTGAGTTGTA
PRIMER_LEFT_1=7,25
PRIMER_RIGHT_1=175,22
PRIMER_LEFT_1_TM=59.408
PRIMER_RIGHT_1_TM=57.790
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=5.9044
PRIMER_LEFT_2_PENALTY=3.592105
PRIMER_RIGHT_2_PENALTY=2.312305
PRIMER_LEFT_2_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_2_SEQUENCE=ACTCCACCTGTTGTTGAGTTGTA
PRIMER_LEFT_2=7,25
PRIMER_RIGHT_2=176,23
PRIMER_LEFT_2_TM=59.408
PRIMER_RIGHT_2_TM=58.688
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=6.0981
PRIMER_LEFT_3_PENALTY=3.592105
PRIMER_RIGHT_3_PENALTY=2.506003
PRIMER_LEFT_3_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_3_SEQUENCE=CTCCACCTGTTGTTGAGTTGTATT
PRIMER_LEFT_3=7,25
PRIMER_RIGHT_3=175,24
PRIMER_LEFT_3_TM=59.408
PRIMER_RIGHT_3_TM=59.494
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=6.2924
PRIMER_LEFT_4_PENALTY=3.592105
PRIMER_RIGHT_4_PENALTY=2.700295
PRIMER_LEFT_4_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_4_SEQUENCE=CCACCTGTTGTTGAGTTGTATTTG
PRIMER_LEFT_4=7,25
PRIMER_RIGHT_4=173,24
PRIMER_LEFT_4_TM=59.408
PRIMER_RIGHT_4_TM=60.700
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_00605 Ps_contig_mira-and-tgicl-ass_franssen_5856
SEQUENCE=ATTTTTATTCTTGTTTCTATGCATGTTGTTTAAATTTAATTGTGCAGATGTTGAACTATACAGAAGATAACAAATTAAGAATCAAGTTACTTTTCGTAGGGTGTTATTATTATTATTTTTTCTTTTGGTTCTGTAAGGGTGTTATTTTTTGTTATAATTATTTTGTAACACACCTCCTATTTTATGGGAGAAGCTATTTATTGTTGTTTAGCTATTCATCAGAAGAGCACACGTGT
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.7945
PRIMER_LEFT_PENALTY=3.473595
PRIMER_RIGHT_PENALTY=0.320870
PRIMER_LEFT_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_SEQUENCE=CACGTGTGCTCTTCTGATGAAT
PRIMER_LEFT=78,25
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=59.526
PRIMER_RIGHT_TM=60.321
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=4.3694
PRIMER_LEFT_1_PENALTY=3.473595
PRIMER_RIGHT_1_PENALTY=0.895776
PRIMER_LEFT_1_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_1_SEQUENCE=ACACGTGTGCTCTTCTGATGAA
PRIMER_LEFT_1=78,25
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=59.526
PRIMER_RIGHT_1_TM=60.896
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=4.5141
PRIMER_LEFT_2_PENALTY=3.473595
PRIMER_RIGHT_2_PENALTY=1.040498
PRIMER_LEFT_2_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_2_SEQUENCE=CACGTGTGCTCTTCTGATGAA
PRIMER_LEFT_2=78,25
PRIMER_RIGHT_2=234,21
PRIMER_LEFT_2_TM=59.526
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=4.7930
PRIMER_LEFT_3_PENALTY=3.473595
PRIMER_RIGHT_3_PENALTY=1.319419
PRIMER_LEFT_3_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_3_SEQUENCE=CACGTGTGCTCTTCTGATGAATA
PRIMER_LEFT_3=78,25
PRIMER_RIGHT_3=234,23
PRIMER_LEFT_3_TM=59.526
PRIMER_RIGHT_3_TM=60.319
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=5.0926
PRIMER_LEFT_4_PENALTY=3.473595
PRIMER_RIGHT_4_PENALTY=1.619006
PRIMER_LEFT_4_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_4_SEQUENCE=TCTCCCATAAAATAGGAGGTGTG
PRIMER_LEFT_4=78,25
PRIMER_RIGHT_4=190,23
PRIMER_LEFT_4_TM=59.526
PRIMER_RIGHT_4_TM=59.381
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_00606 Ps_contig_mira-and-tgicl-ass_franssen_5877
SEQUENCE=GATAATKAAATAAGTTTAGGAAAGAAACCGAACAAACTCCATCTTCTTCCTTGCAGTTACACTTGTGATACTCTAACCTCCAACCACTCCTACAAAAAAAATGACTCTATTTTCTTCACTGCAAATCACCACCACCGTCTCTCTTCACTCTTTCAATCCCTCCTCAACCATCTTCCACAAATCCTTTCTTCCTTGTTTCGCAACTTTACCATCTCTTTCTCTCAATAGATTCGTCTTTTCCACTCAAAAATCTCGAACAACTACTACTACTACCACCCGTGCTTCTTTTCATGAAAATCCAGTTCTATGGGTTGGTAGAATCTGCGTCTTCTATGCTCTTCTCAAGACTGGATTAGTCGGATCTCCATCAAATCCGTTCCTCTCAGATTTGGAAATTGGTAGTAATGGTGATAGTAATGATTCTGGTGACTTAGGGTTCTCTAAATGGACACAGTCCTTACTTGGAAAACCAGCCAAGGAAGAAGCTAACGGAGGGAACCTTACGAGCAAATGGCATCCTACAACAAAGGGTACACTGAGGAGGAACTACAGAGTCCCATCCAAGTCTGAGGGGCGACGACTTCTCAAAGCCATTGCATCTCTGTTGTCAGATGATGACCACTTTGTTGATGCCACTTCTCACAAGGGTTGCCAAATCCGGCGAGAAAGTGCTCATGGAGAAAGTGTTTGTTGCAACAACGTGAGAGCTCTTTTTGATGAGCTACCAACTCCTCATCTCATTGTGGAGATCACTCCTTTTCCTGTTGGACCTCTTAGTGACAAGGATTACACTAAAGCTGAGAAGCTTGAGAAGGTTCTCAGATCTAGCCCTTCTGTTTGAACCCTCCTTGATGTTTCTCGTTTTCATGTATATATTCTTTGCTAGCTTGTAAATATTGGCACCACGAAGAGAAATTTGTATAACATGTTTTACATTTGAGTTGAATCATAATCACATCGTTCAAAGACAGATAACCTGTTACCTTACTTTGTAGACGGATCTGTTCACCTTAAATCGACCAAGGAGAATGAATGTCTTGTARTAAAACT
TARGET=260,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00607 Ps_contig_mira-and-tgicl-ass_franssen_5885
SEQUENCE=AAAACAAAATTACTCAGCCAAGATACTCAAACAAACAAACTTTAAGGAAACCAAATACTTATATGAATTATAAGAAGAACAAAAGGTAGTCAGTGGTTCAGTATCTGCTATAGATTCACAGCAGCTTAATGATACCTCAAGCCTTTACCTCATTGGTATATTAGCATCAGTGAACATTGCAGTGTTTCTATTTGAAATAGCAAGTCCTGTAAGGGGTAATGACTTAGAGCTGTTTTCACTTCCATTACTATATGGAGCAAAGATAAATCATTTGATCATGGTTGGAGAATGGTGGAGGCTTATAACACCAATGTTTCTGCATGCAGGAATATTTCACTTGGGTCTAAGTTGTTGGGCCCTTGTATCATTTGGGCCTCAAGTTTGCAAAAGCTATGGTTCACTTACATTTTTCTTGATCTACATATTGGGTGGAGTTTCTGGCAACATCGCAAGTTTTCTACATACACCAAATCCAACTGTTGGTGGGACTGGGCCTGTATTTGCAATAATTGGTGCTTGGCTCATATACCAAATTCAAAATAGAGATGTTATTGCAAATGATGATTCTGAGAATCTATTCAAGAAGGCTATAATTATCACAATTCTTGGTTTCATATTATGCAACCTTGGTCCAGTTGATGAGTGGTCTCACTCAGGCGCAACTTTCACAGGCATGGTATATGGTTTTCTAACTAGTTCAACTCTTCAGCTTGATAGTACATCGCCACAAACCGGTGAAGAAGGACTCAAACTTGTTAAAAAATACGGTGGTTCTTTCAAATCACTAATTATATTCGTTATATCCATAGTTGTTTTAAGCTCATTACTTTTGTTTATGGATCCCCCTCTCAATGCACTGGCGTCTGCTGATGAAGTAGCTGTTGTAGCCTTAGAATATATGTCTAATTTTTTATTGACTGTCATGAGAGAATCTGATACTTATCAACTTAAAAGTTAGGGTTACTAATTACAGATATNTGATTGTATAAGCTCTACGTTCCGTTTCGGTAACCGTTAAAAA
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00608 Ps_contig_mira-and-tgicl-ass_franssen_5886
SEQUENCE=AGTTTTCTTGCACTTTTTCATTGGATAACGATGATGATGATGATGATGATGATGATTCCTCTACATCAACTTTCCTTTTCTAGCTCTTTTTCTAACTCCTATTTCTTCCATTGCATAGTCCTCTTCCTCCCATCATTTTTAGTTATGTTGAATTCAGCACATGTTTGTTGGTTGACAAGTTAGTATTTCTTATGATTTTCTTTTTGGGTAKTGTTTTTTATTGAGGAGGAAAGTAGAAGATCTTGGCCGTA
TARGET=30,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00609 Ps_contig_mira-and-tgicl-ass_franssen_5889
SEQUENCE=TAGAGAAGAGAAACCGCCTAAGCATCGATCTAGGTGATTGGTGGTGGTTTGTGTTGGTCGGTGGTTATAGTTGCCGGAGATGGGATATTCCGGTGAAGGTGAAAGGTTATGATGTTTTTTTGTAAWTATTAATTACTTTAGGTTTGTGGGGTAGTAGTGCCTAGTGGTTGAGTTGAGTTGAGTTCATCATGAATTTGTATTGTTAATTAAGAACATAATGATGATGGGTCATGTTTTATYTTTCCTTTTGCTACCTTTATTATTACAATAGTATTTTAGTAGTATTAAACTTTTTGTTTATGTAGTCTACAAACTCTTAATACTCTTAATTGGATTGGATAAATAG
TARGET=167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00610 Ps_contig_mira-and-tgicl-ass_franssen_5892
SEQUENCE=GGATTGTGCCGTGGAGATTCTGACGACCGTATTTGGATTCGAGTAACCCGGTTATCGCCGGAGCATGAAACGTGGGTCCATTTACATGGATCCGGGTCGGACCAACCGAGTGAATCGGGCAGGTTGATATTTCTTTTGAGAGATAACATGACTGAAGTGTCGTCGTTTTGGGAGTGAGTGAATAGGGTAATAATGAAGAGGAAGAAAGAAAGAGTTAAAGGGTTTAGTACTATGATGTTTTTCTTTATGTTGTTGTTGTTGTGGAAGAGAAGAGAAGAGGAACGATTTTTCCTTATCTTTCTATCTTCATTTTTATGTGGAGTGAGGTAGGTGGAGAGAAATGGAGGGTTGTTGGTTCGATTTGTGGAAGATGAGAGGTGGTCGGGTGTATTTGTGTAAGGAAGACGACGGCGGAAAGAGGATGGGTCAGCGGTGTTTGTCGGCGTCGTGGAGGTGTGGGAACGGCGGAAGGCAACGGAGGGTATGATGAGTAGAGAGTGGACGTCGCGGAGATGAAGGTGAAGGGTTTGCGGCGGTGGGTAGATGGGGTTTGTGTGTCGTTGGTGGCGAAGAAGAAAAACTTCTTCTTATTTCCTGTTTTTCCTTTTTCAGCTGAGAGAGGAAAGAGAGATGAGTGGAGGTGAGATGATGAGAAGCGAAGAGGAACCATCCTCTCAACTCCCTTTTTCCTTATTTTTCTTCGTGAGTTCAGAGGTTCCAAGAATCCACCCAAAATAAATCATCCTCTCCCCTTTTGAATTGGAAAAGTCCACTTCTTAAAAATAGTATTGTATCTTATTTTGAAAACAGGTTACCAATACTTTTTTCTGTTCTGAAAATAAGGCCCCTTTTCCATTCTTGTGTCACATGGATTTCCAATTTTGTGGACTGCTTTCCTCTACCTCCCAAAAGCAAGATGTCGCACGTAATGTTTTTGTGTCGYSTMC
TARGET=202,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00611 Ps_contig_mira-and-tgicl-ass_franssen_5892
SEQUENCE=GGATTGTGCCGTGGAGATTCTGACGACCGTATTTGGATTCGAGTAACCCGGTTATCGCCGGAGCATGAAACGTGGGTCCATTTACATGGATCCGGGTCGGACCAACCGAGTGAATCGGGCAGGTTGATATTTCTTTTGAGAGATAACATGACTGAAGTGTCGTCGTTTTGGGAGTGAGTGAATAGGGTAATAATGAAGAGGAAGAAAGAAAGAGTTAAAGGGTTTAGTACTATGATGTTTTTCTTTATGTTGTTGTTGTTGTGGAAGAGAAGAGAAGAGGAACGATTTTTCCTTATCTTTCTATCTTCATTTTTATGTGGAGTGAGGTAGGTGGAGAGAAATGGAGGGTTGTTGGTTCGATTTGTGGAAGATGAGAGGTGGTCGGGTGTATTTGTGTAAGGAAGACGACGGCGGAAAGAGGATGGGTCAGCGGTGTTTGTCGGCGTCGTGGAGGTGTGGGAACGGCGGAAGGCAACGGAGGGTATGATGAGTAGAGAGTGGACGTCGCGGAGATGAAGGTGAAGGGTTTGCGGCGGTGGGTAGATGGGGTTTGTGTGTCGTTGGTGGCGAAGAAGAAAAACTTCTTCTTATTTCCTGTTTTTCCTTTTTCAGCTGAGAGAGGAAAGAGAGATGAGTGGAGGTGAGATGATGAGAAGCGAAGAGGAACCATCCTCTCAACTCCCTTTTTCCTTATTTTTCTTCGTGAGTTCAGAGGTTCCAAGAATCCACCCAAAATAAATCATCCTCTCCCCTTTTGAATTGGAAAAGTCCACTTCTTAAAAATAGTATTGTATCTTATTTTGAAAACAGGTTACCAATACTTTTTTCTGTTCTGAAAATAAGGCCCCTTTTCCATTCTTGTGTCACATGGATTTCCAATTTTGTGGACTGCTTTCCTCTACCTCCCAAAAGCAAGATGTCGCACGTAATGTTTTTGTGTCGYSTMC
TARGET=248,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00612 Ps_contig_mira-and-tgicl-ass_franssen_5892
SEQUENCE=GGATTGTGCCGTGGAGATTCTGACGACCGTATTTGGATTCGAGTAACCCGGTTATCGCCGGAGCATGAAACGTGGGTCCATTTACATGGATCCGGGTCGGACCAACCGAGTGAATCGGGCAGGTTGATATTTCTTTTGAGAGATAACATGACTGAAGTGTCGTCGTTTTGGGAGTGAGTGAATAGGGTAATAATGAAGAGGAAGAAAGAAAGAGTTAAAGGGTTTAGTACTATGATGTTTTTCTTTATGTTGTTGTTGTTGTGGAAGAGAAGAGAAGAGGAACGATTTTTCCTTATCTTTCTATCTTCATTTTTATGTGGAGTGAGGTAGGTGGAGAGAAATGGAGGGTTGTTGGTTCGATTTGTGGAAGATGAGAGGTGGTCGGGTGTATTTGTGTAAGGAAGACGACGGCGGAAAGAGGATGGGTCAGCGGTGTTTGTCGGCGTCGTGGAGGTGTGGGAACGGCGGAAGGCAACGGAGGGTATGATGAGTAGAGAGTGGACGTCGCGGAGATGAAGGTGAAGGGTTTGCGGCGGTGGGTAGATGGGGTTTGTGTGTCGTTGGTGGCGAAGAAGAAAAACTTCTTCTTATTTCCTGTTTTTCCTTTTTCAGCTGAGAGAGGAAAGAGAGATGAGTGGAGGTGAGATGATGAGAAGCGAAGAGGAACCATCCTCTCAACTCCCTTTTTCCTTATTTTTCTTCGTGAGTTCAGAGGTTCCAAGAATCCACCCAAAATAAATCATCCTCTCCCCTTTTGAATTGGAAAAGTCCACTTCTTAAAAATAGTATTGTATCTTATTTTGAAAACAGGTTACCAATACTTTTTTCTGTTCTGAAAATAAGGCCCCTTTTCCATTCTTGTGTCACATGGATTTCCAATTTTGTGGACTGCTTTCCTCTACCTCCCAAAAGCAAGATGTCGCACGTAATGTTTTTGTGTCGYSTMC
TARGET=264,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00613 Ps_contig_mira-and-tgicl-ass_franssen_5895
SEQUENCE=CAAAGCAATTTTGGTAGCTGATATACCTTCTAAGCTATTGAAGCTTTGGAGAAAGGAAGAATCCAAAATTTGCAGAAGATGGTGCTAATGATGCTGCTAATGAAGCAAGTTATTGTGAGAGTGAGTTTAATGGGAAATCACCACTTACTATACAGAACAATGCTATGCATGTATTGTTTTCTAGTTTGTTACTGCAGCTTAGTTGGTTAGACTAGTTTAACTTTTAAGTTTAGTTTTATGTATTTATTACTTAATTAAAGTTTAATTTAATTTAATTATTGTTTATTGTTCTTAATAGGTAAAATTTAATTTAGGTTAAGTTAGTTTAAATTATAGTTAAGTTGTTCTTACTTAATTACTTAAATAAAGTTACACGCTATTA
TARGET=261,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00614 Ps_contig_mira-and-tgicl-ass_franssen_5896
SEQUENCE=TTTTCCAGCGGCATCAAGACCAAGCATTTGTACAACCCGCATCTCGGTATTGCCGAAGAAGGTATCGAAAAGCTTGCGAAAARCTTGACCCATTCGATCAAAATCAAAAGAAAATCGTTGCTTGATTGAATATACRATTCTTGGAAAACCGAAAAACTAGGTTTTTTGGATTTACAAAAATGACAAAGGGAAGGGAAAGGAAAGGGGGATTGAATTGAATTGAATTGCGTTTCCTTCAACCTCCCTGTTGCGTTTGATTTAGGAAAATGAATTCCTCCCG
TARGET=209,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00615 Ps_contig_mira-and-tgicl-ass_franssen_5901
SEQUENCE=TCAACGCAGAGTACGGGGGAAATATATGAGATGTGATGGTAATTTGGCAAGGTCTCTGAGATTTTGCGACCAACCTGCTTCTTCTATCTATCGTCTTCCTCTTTTTCTTCTTCTTTTTCTTCCAAACCATTGTTAGGGTTTCAACTTTCTCTCTCTCGGAGCCTCAAATTAATCCACAATTGAACCCAATTTCGCAACCTTTTCATACTTTTCAGTTCATACAACTCACCTTATCCGAACACATCGACAACTCCGTGTGTTATCTGTCATACACCAATGTTGACCACTAGTGCAGTTGCATCTTTGCCCAAATTAACACCAGTGAGAAGAGGTGGAAACTGCATTGAAGAGAATGTTGTCCCTACATCTATATTTTCAAGAGGATTACTGAATGTTAATAACTCAAGATCATTGAGTCGCACACAGTTTCCATTTGATAGAAGAGMAGACCCGTTCTACTCAGGCAGCAACTATAATAT
TARGET=148,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00616 Ps_contig_mira-and-tgicl-ass_franssen_5913
SEQUENCE=GATCYAGAATGATAATTGTGGTTGTATCATCGTGCAGTTGAGTTCGTTCTGTCCTGTAAGCTTTAACTATTTTCTAAAATACATCATTCAATCGAATTTAAACTAATAGAACATGTTAGTCATCATCATCATCAATCTCTCTTCAACCAACGTGNTTCTTCTTCCACCACTNATCCATCGATTACGACCCCACTACAAAACCAAACCTACNTCAAGATAG
TARGET=120,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00617 Ps_contig_mira-and-tgicl-ass_franssen_5929
SEQUENCE=AATATTTCAATGATCTTTTCKAAGAAAAACGGGAGGATAGTAACACAAATTCAATGGCTCTTTCTTACACCTTCATCACTCCTTCTATACCCTCTCTTTCTCTACACAATCATCATTCATCAAAGCTTCCCCTTAGAGTTTCTTATATAAATGCTTCCTTAAACTCTTCTGATTCAAAGGCACCATCATCATCATCATCATCATCATCATCTTCTTCTTCTTCACCACAAAGTGTGCCATCTTCAACCCCTTATGTTGAGTCTAGGCCACATGAGCCTGCCTTTAACTATGCCTTTGCTAACACTAATGGCAACCCACTTTTTAGGATGCTTCAAAATACAGAATCTTCCATTGAAAGGGTGATTTTCGACTTCCGTTTCTTGGCACTCTTTGCTGTTGCAGGCTCCTTGGCTGGCTCACTCCTCTGCTTCCTAAATGGTTGTGTTTATATTGTTGGTGCATACAAAGTGTATTGGACAAGCTGTGTCAAAGGAGTTCACACTGGAAAGATGGTTCTGCTTCTGGTCGAAGCAATTGATGTTTATCTTGCTGGTACTGTTATGTTGATTTTCGGCATGGGCTTATATGGGCTGTTCATCAGCAACACGCCTCCTGATGTGCCACCTTCTGTCGACCGTGCCCTCAAAGGATCTTCTCTCTTTGGAATGTTTGCTTTGAAGGAGAGACCTAAATGGATGAAAATAAGCTCACTTGACGAATTGAAAACGAAAGTAGGGCATGTTATTGTAATGATTCTTCTTGTAAAAATGTTTGAGAGGAGCAAAATGGTTGTAATTGCAACAGGTTTAGACTTGCTTAGTTACTCAGTATGTATTTTCTTGTCATCAGCATCTTTGTATATTCTTCATAATCTACATAAGCAAGATTAGAATGTAGTAGGTAACTAGGTATTGTAATATTCTGAATTCATATATATGTTAATGGAGAATCATATGCTCTTAATCTACTTTTAAAGTTCTAGCTMTAAAATACACTTACTTAAAAA
TARGET=184,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00618 Ps_contig_mira-and-tgicl-ass_franssen_5929
SEQUENCE=AATATTTCAATGATCTTTTCKAAGAAAAACGGGAGGATAGTAACACAAATTCAATGGCTCTTTCTTACACCTTCATCACTCCTTCTATACCCTCTCTTTCTCTACACAATCATCATTCATCAAAGCTTCCCCTTAGAGTTTCTTATATAAATGCTTCCTTAAACTCTTCTGATTCAAAGGCACCATCATCATCATCATCATCATCATCATCTTCTTCTTCTTCACCACAAAGTGTGCCATCTTCAACCCCTTATGTTGAGTCTAGGCCACATGAGCCTGCCTTTAACTATGCCTTTGCTAACACTAATGGCAACCCACTTTTTAGGATGCTTCAAAATACAGAATCTTCCATTGAAAGGGTGATTTTCGACTTCCGTTTCTTGGCACTCTTTGCTGTTGCAGGCTCCTTGGCTGGCTCACTCCTCTGCTTCCTAAATGGTTGTGTTTATATTGTTGGTGCATACAAAGTGTATTGGACAAGCTGTGTCAAAGGAGTTCACACTGGAAAGATGGTTCTGCTTCTGGTCGAAGCAATTGATGTTTATCTTGCTGGTACTGTTATGTTGATTTTCGGCATGGGCTTATATGGGCTGTTCATCAGCAACACGCCTCCTGATGTGCCACCTTCTGTCGACCGTGCCCTCAAAGGATCTTCTCTCTTTGGAATGTTTGCTTTGAAGGAGAGACCTAAATGGATGAAAATAAGCTCACTTGACGAATTGAAAACGAAAGTAGGGCATGTTATTGTAATGATTCTTCTTGTAAAAATGTTTGAGAGGAGCAAAATGGTTGTAATTGCAACAGGTTTAGACTTGCTTAGTTACTCAGTATGTATTTTCTTGTCATCAGCATCTTTGTATATTCTTCATAATCTACATAAGCAAGATTAGAATGTAGTAGGTAACTAGGTATTGTAATATTCTGAATTCATATATATGTTAATGGAGAATCATATGCTCTTAATCTACTTTTAAAGTTCTAGCTMTAAAATACACTTACTTAAAAA
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00619 Ps_contig_mira-and-tgicl-ass_franssen_5933
SEQUENCE=TAAAAATTGCTTAGGTTTTTTGGGTCTTTCCTCCATTCCTCAAAAGAATCAACGAACCAGGCCTCAGTTTCTTCAGTGCTTTTGCATGTAAAGTCTGGCCTGCTAGATCCACCCCAACCAAGTTGATCAATAGCAACAACTCTAAATCGCGAAGCAAGTGCATCAAAATTGCGAAAGAAGAAACCCTGTGATGCAGCATATCCATGAACCATTACTAGTGTAGGAGAATCCTCTTTACTATCAAAGGTAACAGTGTTGATAAACCGTGGCTCATTGCTAGATGAACGAAACCAGCTCACTCTGGAATCCGGTGGACCAGAACCAATATTAACCTGTTCTTGGACATAAGGAGTCTTGACAAGAGAAAGAAGGCGCTTCTCGGCATTGATGATGTGGTCGGTAGAAGTGGGGATCCAACGGCGGAGAAATGGCCAGAAACCGCCTGATTCGAGGTTGTTTTTTCCGGTGTTTTCTGCCGCCATTTTTGCTAAGAAACGGGATCGAACTGGGTTCATAGAATAACAAACTAGTAACAAATTAACTAACTAAGAAACGAACTTGTTTCTGTGATTGGAAATTGGAAATGATTATTACAACTTGGAAGAATGAATGAATGAAGAAGAAGGAGCAGTGAAATTGAGTTTTGACTGTTMCCGCGTACT
TARGET=604,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00620 Ps_contig_mira-and-tgicl-ass_franssen_5938
SEQUENCE=GGTGCATATATTAATAATAATTTAAAAATTGTTKGAGAGTTGAGAGAGTAGCGTAGTGTTGGCAATTGAAGTTCGTTTCTGGATAATAATGTTGATAACCGTAAGAGACCCTTCCCCTACTCTCCGCTGTTCTTCGCTTAACCGCCGTCTCATTCCAACAAAACACAACCTCTCTCCCCGTCTTCTCCGTTCATCTTTCCGTCAACACAACCTCTCTCTCCGTCTCCGTTCATCCTCCTCCTCCAATCTCTATACCAGTTGTGACCTAGCGAATTTCGATCCTCTAGGCATTAATCCAGATTTATCTTCATCTTCTACTTGGAGAAATCTCCTCACTATCTTTCAAACCTCTTCGCAAAAAGATAAACCTCGAGGCGTAGCAGCTGCAATTGAGGATAGTTCAATTGATTTTGGTGACTTCTTTAATGGTCCACTGCCTGGGAAGTTTCTCAAGCTTTTAGGGTTTCTTGCTTTGTCGAGGCTCGGTGTGTATATACCTCTGGGTGGAGTTAATAGGGACGCTTTTCTAGGAAATTTGGATCAGAACTCTTTGTTAACTACTTTGGATTCCTTTTCTGGTGGAGGTATTGGTAGACTTGGAATATGCTCTCTTGGCATTGTTCCGTTCATCAATGCGCAGATTGTTTTTCAGCTTCTTGCTCAAGTGTATCCCAAATTGCAAGATCTTCAGAAGAAAGAAGGTGAGGCGGGAAGAAAGAAGCTTCTTCAGTATACTCGATATGCTTCGGTTGGATTTGCTATTGTACAGGCTATCGGGCAAGTTCTTTTCCTACGGCCCTATGCAAATGATTTTACTACAGAGTGGGCGCTTACTTCTGTTATCTTACTAACACTTGGCTCTGTCTTTACTACATACATTGGGGAACAAATTACCGAACTCAAACTTGGGAATGGTACCTCTCTTTTGATATTCACAAACATTATCTCATACTTGCCGGCGTCTTTTGGTCGCACATTTTCACAGGCGTTTAGTGATGCTAACTATGTTGGACTTGTTACCATCATTGTCTCATTCTTTCTGTTGGTACTTGGTATTGTCTATGTTCAGGAAGCAGAGAGAAAAATTCCTATTAATTATGCTTCTAGATTCACCAGCAAAAGTGGAGGGATTGAAAAATCGGCTTACCTTCCGTTTAAGGTAAATAGTTCTGGGGTAATGCCTATCATTTTTTCTACATCATCATTAGCTCTTCCTGGTACTTTAGCACGCTTCACTGGTTTAAGCTCACTGAAAACTGCTGCAGTGGCTTTGAATCCAGGAGGCTCCTTCTATCTTCCATTTAACATACTTTTGATTGCCTTCTTCAACTACTATTATACTTTCCTACAACTGGATCCCGATGATGTGAGTGAACAATTGAAACGTCAAGGTGCTTCAATTCCACTTGTCAGACCAGGCAAAAGTACAGCTACATTTATTAAAACAGTTCTTAGTCGAATATCAGTTCTGGGTTCAACATTTTTGGCAATCTTGGCTGCTGGACCTGCAGTTGTTGAGCAGACCGCACACTTGACTGCATTCCGGGGGTTTGCTGGAACATCTATTCTTATTCTTGTTGGTTGTGCGACAGACACGGCCAGAAAAGTTCGAGCTGAGATTATATCCCAGAAGTACAAAAACATAGAGTTATATGATTTTGATAAGTATTGACCATTTAACAATTGGTTGTTTCCTTTGTCTAAACACACTACTAGATCATTTTATATTGACCTGTATATAATTTTGCAGAGCATTATTTTTTTATTATTTTATCTGTAATATAAAGATTAATTTGAATACATACAATTGCTTGCGWAGAACTAGTTTAATCAATCGTTGGAACTTATGG
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00621 Ps_contig_mira-and-tgicl-ass_franssen_5941
SEQUENCE=GTCGGGGTAACCTTTATTTCTAGATTCTAAGKTCGGGGATGTTATCGGCATATACTAGGAATCTCATCTCGCTTCTGTCACTGCAACCGTGCATCTTAAGTTAGAACGACACAAAAAGCACACAAAAACAACATTGTTGAATCTTAAACACTATAAATCAATTCAATTCAATCAAGCATTGTTGTAAAGTTTGAGTTTTGAGTTTAAAGATCTGAGCTTTGTTGATGGATAGTTCAGAGAAGGTTGTTGCTGTGATTATGGTGGGTGGACCCACCAAAGGAACTAGATTTCGACCTCTTTCTTTCAATACTCCAAAACCCCTTTTCCCTTTGGCTGGACAACCAATGGTTCATCATCCTATTTCTGCTTGTAAAAGGATACCCAATTTAGCTCAAATATATCTCATTGGATTTCCTTACGAGGAGCGAGAATT
TARGET=158,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00622 Ps_contig_mira-and-tgicl-ass_franssen_5988
SEQUENCE=GGGAAGCACCTRRGGGTAGAGAAAAGGACGAGTTATTATCATTCCAAAGAAAAGAAGGTAGAGAAAAAAAGTGTGATTTGTGGAGCAACAATGGCAACACCAAAAAGATCATATGATCAACAACAACAACAACAAATTGTGCAACAAGTGAAGAACTCAGGAATGATTAACAACATTCAGAGTCCAAGAAGAGATGAAAGAGAAGAGGAAATGTCAAGATCAGTTCTAGCAATGTTTAGAGCAAAGGAAGAAGAAATTGAGAGGAGAAAATTGGAGGTTAGAGATAAAGTTCATGCTCATCTCGGTCGAGTACGAAAAGGAAACAAAGGAGGGTTGGCTGAGATAAGAGAAGAGATAGGTAGGGTTTCACAGACTACCAATGGAAGGAAAGTNTTGGTT
TARGET=118,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00623 Ps_contig_mira-and-tgicl-ass_franssen_5997
SEQUENCE=GAACACAAATATAAAAGTAATGTTAACGTGAGATATTTATATAGTATATAAAAATTCAACCCAGAAGAAAACAGATATTAATAATAATAAAAAAAACAATAGGAATATTTCTGGATCTGAGAAACCAGAAACTCATTCATCTTACTCACGCAAAAGGGTTCTCACTCTCGACATCTTTCAAACTCTCAATCTCTCGTTAACCCTCAATCGGCTTGCGCCAACCTCACGGTACGCCACTCCCAATCTCACTCTAAACGCGAAAACCATGGTTTCAAAAGAAGATCCAGTAAGAAGACTCACCTTCGGCGGTGATTCGGCGGCGATGCTAGGGTTTCAGATTGGCCCAAGGAGATCAAAAGGGGTCTCTGCGAAATCCCCCTTAGGGTGCTCAGAATTTTGATTGCTCTATTGATGTTTGGTCTGGAACGTGAAAACTTGGTGAAAATTAAAACTTCGGTTATTTGCAAAGCTTGGTCTTGGAAGTATCGCGTTTGAGGTTATGGCTGGGACACGGTTTTAGCTACAGGCATTTTTTTTAAGTAACACCCTCAATTGATTAAGAATGACTTTTGCATTACCGGAGAATCATATGCCGGACACTATATTCCAGCTCTCGCATCCCGGGTTCACAACGGAAACAAAAACAAAGAAGGAATTATTATAAACTTCAAGGATTTTGCCATTGGTAATGGATTGACCAACCCGGAAATTCAGTACCCAGTATGTACTTAGTTTGCAGTTGATAACAAACTGATCACAAAGGAAGATCAAGCAGATATCATCAAGTTGATCCTATTTCGTGTAGGTGCCGCAAAAAATTGTGTGAGCAAAGGTGGAGAAAGTTGCGAAACAACCTTATTACAATGTCAACCGATAGTCACTAATATCTTAGCCATTGCTGGCAATTAATTACTACGACATCAGGAAGAAATGTGAAGGAGAATTGTGTTATGACTTCTCAAGTTGGGGAAATAAGAAGACCGTTACGGATGCGATGCGTTGGGTTGGTTGGGAAAAGTAAGAGGTTTGGTAAAGTAGTTTGTAAAGTTTAATTATTACCGAATGTTATTAGAAATAGTGTGTATGCGCT
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00624 Ps_contig_mira-and-tgicl-ass_franssen_6001
SEQUENCE=TCSGGGGTACTTAAGTTCGACAGTTGTTGGATACGAAACAAACAAGCAACAAATTCATTCATTCAGATATGATTTGAGAAACCAAAACCGACGAAATTGAAGCATACCGTTGAAGAGTAAAGTGTTGTAGAATTACAAGAATCACAATCGATGGCCACTGCAACAACAACAACAACAGTAGTAAAGTATGGAATAATAGGCGTTGGAATGATGGGAAGGGAACATCTCATCAATTTGTATCAT
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00625 Ps_contig_mira-and-tgicl-ass_franssen_6001
SEQUENCE=TCSGGGGTACTTAAGTTCGACAGTTGTTGGATACGAAACAAACAAGCAACAAATTCATTCATTCAGATATGATTTGAGAAACCAAAACCGACGAAATTGAAGCATACCGTTGAAGAGTAAAGTGTTGTAGAATTACAAGAATCACAATCGATGGCCACTGCAACAACAACAACAACAGTAGTAAAGTATGGAATAATAGGCGTTGGAATGATGGGAAGGGAACATCTCATCAATTTGTATCAT
TARGET=161,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00626 Ps_contig_mira-and-tgicl-ass_franssen_6025
SEQUENCE=ACTTCATGAAGGTTCGAGATTCTATCGTTAACCGGTAAGGCTTTCTTACCAGGTCCGGCCGTCCTCCCCGGTCGCTACCGGACTCACAGTTTACTTAGCTGGTGGACAAGGGCAGGTTGTTGGCGGAAGTGTTGTTGGATCTTTGATTGCTGCTGGACCTGTTATAGTTATCGCCGCTACTTTTGCTAATGCTACTTACGAGAGATTACCACTTGAGGAGGAAGAAGACGATGACGGAGGCGGGATTCACGGAGGACATCATGTTGGTGGTAATAATAATAACTCATCTCCTCCAGGAATTGGAAGCGGCGGCGGCAGTGGCGGTGGTGTGGGACATATGCAGTCTGGCCTTCCAGATCCATCTTCTTTGCCTTTGTATAACCTGCCACCAAATCTTCTCCCTAATGGTGGCCATGTGGGACATGAAGCTTTTCCATGGCCTCATGGAATTAGGCCACCATACTGAAACAAATGATATGAATCTGATGACATTAGTAAATTGGATAWTTATWTATATAGTTATTATTATATTGTTAGTTGTTTTAGTTGTAGTAATTAAAGTGGTAGTAAGTAAAAGGAAAGGTAAGGTAAGGAAAGG
TARGET=271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00627 Ps_contig_mira-and-tgicl-ass_franssen_6041
SEQUENCE=AAGGGTGAAACGGAACGGTAAGGGTCTGTATTTTTGGGTAGGTAATGAGCAGGATCCGTTGGATCAGAAGGGTTAATAACGTTTATCTTTTGAACACGTGGGACGGYTTGAACCGTTGCTAGTCGATAAGTAAGAGTTTGTACGGATCAATTGGTAACCCTTGGATTGGGTTAGTGCTCTTTCATCTGGACCGATGATTTGGTATCTGGCACGTGCTTTTGAAATTAATTGAAACTCCTAGTTTCCTTCATTTATTTATTTTGATGAGACACCAAACAATTGTATACCCGTAAAACTAAATCTTCTATGCATATAAAATGGAAGAGAAAAAGGGTGATGAATAGTGTAAATTAATTTTACAAACT
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00628 Ps_contig_mira-and-tgicl-ass_franssen_6052
SEQUENCE=CAGGTCCACCCTCGAAGGCGACTCCGAACAAGTTTCTACCGCTTTGGCTCTTTCCCCCAACGATAAACTTCTCTTCTCTTCTAGCCACAGCCGCCAAATCAGAGTTTGGGACTTATCCACTCTCAAATGCGTGCGCTCGTGGAAGGGTCACGATGGTCCTGTTATGTGTATGGCTTGTGATGCTTCTGGAGGTTTACTTGCTACTGGTGGAGCTGATAGGAAGGTCCTTGTTTGGAATGTTGATGCTGGATATTGCTCACATTTCTATAAAGGTCATGGTGGAGTTGTTTCT
TARGET=68,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2362
PRIMER_LEFT_PENALTY=0.162217
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=GAACAAGTTTCTACCGCTTTGG
PRIMER_RIGHT_SEQUENCE=TCAGCTCCACCAGTAGCAAGTA
PRIMER_LEFT=25,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=60.162
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.2385
PRIMER_LEFT_1_PENALTY=0.164560
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=CGACTCCGAACAAGTTTCTACC
PRIMER_RIGHT_1_SEQUENCE=TCAGCTCCACCAGTAGCAAGTA
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=60.165
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.2578
PRIMER_LEFT_2_PENALTY=0.162217
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=GAACAAGTTTCTACCGCTTTGG
PRIMER_RIGHT_2_SEQUENCE=ATCAACATTCCAAACAAGGACC
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.2601
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=CGACTCCGAACAAGTTTCTACC
PRIMER_RIGHT_3_SEQUENCE=ATCAACATTCCAAACAAGGACC
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.2601
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=GACTCCGAACAAGTTTCTACCG
PRIMER_RIGHT_4_SEQUENCE=ATCAACATTCCAAACAAGGACC
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_00629 Ps_contig_mira-and-tgicl-ass_franssen_6062
SEQUENCE=CCGTCCTTGCGCCCGGGCCGTAAATACCCCTTTTCGGTAGATGCTTTCAGTGGTCCTGAGATGTGGGCTAAGCTGACGGCTGATCCTACAACTAGGGCTTTTCTTCAACAACCTGATTTTGTCAAGATGATGCAAGATATTCAGAAAAATCCGAATAATCTTAATTTGTATCTGAAGGATCAAAGAGTTATGCAAGCTTTTGGGGTTTTGCTTAATGTTAAGCTTGGAAGTCCTGGTGATGATGTGGACATGCCTGATACTCCTTCTTCCTCTTCTTCTGAGAGGAAGAGAGCGGCTGAAGCTGAGCCTCCTAAGCAGCCTGAGCCGGAGCCTGAACCTGAACCCATGGAAGAGGCTGATGAGGAAAAGGAAGCTAAGCAGAGAAAGGCTGAGGCACAGAAGGAAAAGGAGGCTGGTAATGCAGCGTACAAAAAGAAGGATTTTGATACTGCAATTCACCATTACTCAAAAGCTTTGGAATTGGATGATGAAGATATATCGTTTCTCACCAACCGTGCTGCTGTATACTTGGAGATGGGAAAGTATGAGGATTGCATTAAAGATTGTGACAAGGCAGTTGAAAGAGGACGGGAACTACGATCAGACTACAAGATGGTAGCAAGAGCTTTGACAAGGAAGGGAACTGCCATGGGCAAAACGGCAAAATGCTCTAAAGATTATGAACCTGTCATTGAGACATTTCAAAAAGCACTTACCGAGCACCGCAACCCAGACACCCTAAAGAAACTGAATGAAGCTGAAAAGGCCAAGAAAGAATTAGAACAACAAGAATACTTTGATCCAAATTTGGCTGACGAGGAGCGAGAAAAAGGGAATGAATTCTTTAAGCAGCAGAAGTATCCCGAAGCTATTAGGCATTACACCGAGTCTCTAAAAAGAAACCCGAAAGATCCTAGGGCTTATAGTAACAGAGCTGCATGTTACACCAAATTGGGGGCAATGCCTGAAGGTTTAAAGGATGCAGAGAAGTGCATTGAGCTTGATCCAACCTTCACCAAGGGTTATACCAGGAAAGGTGCAGTTCAGTTTTTCATGAAAGAATATGAAAAGGCTTTGGAAACATATAAGGAGGGGTTGAAACATGATGCCAACAACCAGGAGTTGCTTGATGGCATTGGAAGGTGTGTACAACAAATTAACAGAGCCAGCCGTGGAGATTTAAGTCCTGAAGAGTTGAAGGATAGACAGGCCAAGGCAATGTCAGATCCAGAGATACAGAATATCCTTCAAGACCCTGTTATGCGACAGGTATTGGTTGACTTCCAGGAAAATCCCAAGGCTGCACAGGAACATACCAAGAACCCGGGTGTGATGGACAAGATCCAAAAGCTGATCAGTGCTGGAATTGTCCAGATGAGGTAAACTTTGTGGAAGATGGCAGCTCTCGCATAAAAGCAATTCCTACAGTGACTTTAATTTGATATTTGATAGATTGGATTGGATGTAAAATGGGAAATTTTGACTTTGTTTTTCAGTTTGTTTTCGTGATTTTTTATATTTTATAACAAACTCGAGTGCAATTCATTTTAGCATAGTAGTAGTAGTTGGTTTCGTATGTATTGACTTGGCTTTTTATAATTTAATCTTAATCCGTAWCTAAACGT
TARGET=1554,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00630 Ps_contig_mira-and-tgicl-ass_franssen_6088
SEQUENCE=GGCTATGGTTGGGGACTTGAGAAAAGAAAGAAAAAATTGAGATAGAAGAGAAAGATGAAAACATTATCATCATCTTCATCACATTCTTTCAATTTCATTCACCCTTCCAACTTCAACTATTCTTCTTCTTCATCTTCTGCATTTTCTCCTTCTTCT
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8348
PRIMER_LEFT_PENALTY=0.213575
PRIMER_RIGHT_PENALTY=2.621188
PRIMER_LEFT_SEQUENCE=TGGGGACTTGAGAAAAGAAAGA
PRIMER_RIGHT_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT=9,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=60.214
PRIMER_RIGHT_TM=57.379
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=2.8856
PRIMER_LEFT_1_PENALTY=0.264444
PRIMER_RIGHT_1_PENALTY=2.621188
PRIMER_LEFT_1_SEQUENCE=TTGGGGACTTGAGAAAAGAAAG
PRIMER_RIGHT_1_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=59.736
PRIMER_RIGHT_1_TM=57.379
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=2.9657
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=2.621188
PRIMER_LEFT_2_SEQUENCE=CTATGGTTGGGGACTTGAGAAA
PRIMER_RIGHT_2_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=57.379
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=3.0114
PRIMER_LEFT_3_PENALTY=0.390220
PRIMER_RIGHT_3_PENALTY=2.621188
PRIMER_LEFT_3_SEQUENCE=GTTGGGGACTTGAGAAAAGAAA
PRIMER_RIGHT_3_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=154,22
PRIMER_LEFT_3_TM=59.610
PRIMER_RIGHT_3_TM=57.379
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=3.3144
PRIMER_LEFT_4_PENALTY=0.693167
PRIMER_RIGHT_4_PENALTY=2.621188
PRIMER_LEFT_4_SEQUENCE=TATGGTTGGGGACTTGAGAAAA
PRIMER_RIGHT_4_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=154,22
PRIMER_LEFT_4_TM=60.693
PRIMER_RIGHT_4_TM=57.379
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_00631 Ps_contig_mira-and-tgicl-ass_franssen_6092
SEQUENCE=CCTTTCGTCTCCGTTTCCTATCGCAAAACCCAACCTTTACCTTCTCCAATCACCACTCTCAACCCCTCTCCATTTCTGCCTCACCTCCAAAACCATGCAACCGGCTCGGTTTCGTTTTTTCTCAATGGCTGCTGCTGAGCCGAAGGAATCGCCGGCTAACAACCCCGGTCTCCATGCAACTATAGATGAAGCTACTAAGGGCTACTTTATGCAACAAACTATGTATAGAATCAAAGACCCCAAAGTTAGCCTTGATTTTTACTCCCGCATTTTGGGCATGTCTTTGCTCAAGAGACTGGATTTTCCCGAGTTGAAGTTCAGCTTGTACTTTATGGGGTATGAGGATACATCAGAAGCTCCAACTAACCCTGTTGATAGAACAGTTTGGACCTTTGCTCAAAAGGCTACAATTGAATTGACACATAATTGGGGCACCGAAAGTGATCCAGAGTTCAAAGGGCACCACAATGGCAATTCCGATCCTCGTGGTTTTGGACATATTGGCATAACTGTTGATGACACCTACAAAGCATGTGAAAGATTTCAGAATCTGGGGGTTGAGTTTGTTAAGAAACCAGATGATGGAAAAATGAAAGGGATAGCATTTATTAAGGATCCCGATGGATACTGGATTGAAATCTTTGACCGGAAAACAATAGGAAATGTAACAGAAGCTGCTGCTGCTTAAGATAAAATCTTCAGGTAGAAGCCGTGGTTTCTAATTCCGTTGTCTTATTTACTTATTCACGTTTTTCGTCCTAAAAGTAATGATGTCCCTACTTCATATTCTCTGATGATATAAAGAACCACGTTACCTGCATGTGAAAATTTGTTTCATTGTTCTGAATTATGTATAACCATGTGAAATCGCTTTCTCCAAATTAGTTTGCTATAATGCATATTTGTAGCTCAGAAACAAATCACGTGATTTTG
TARGET=674,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3992
PRIMER_LEFT_PENALTY=0.182645
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=AAGATTTCAGAATCTGGGGGTT
PRIMER_RIGHT_SEQUENCE=CACGGCTTCTACCTGAAGATTT
PRIMER_LEFT=537,22
PRIMER_RIGHT=713,22
PRIMER_LEFT_TM=60.183
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.4755
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.216588
PRIMER_LEFT_1_SEQUENCE=AATCTGGGGGTTGAGTTTGTTA
PRIMER_RIGHT_1_SEQUENCE=CACGGCTTCTACCTGAAGATTT
PRIMER_LEFT_1=547,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.783
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.5351
PRIMER_LEFT_2_PENALTY=0.283783
PRIMER_RIGHT_2_PENALTY=0.251350
PRIMER_LEFT_2_SEQUENCE=GACCGGAAAACAATAGGAAATG
PRIMER_RIGHT_2_SEQUENCE=GCAAACTAATTTGGAGAAAGCG
PRIMER_LEFT_2=643,22
PRIMER_RIGHT_2=889,22
PRIMER_LEFT_2_TM=59.716
PRIMER_RIGHT_2_TM=60.251
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.5394
PRIMER_LEFT_3_PENALTY=0.322824
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=GCATTTATTAAGGATCCCGATG
PRIMER_RIGHT_3_SEQUENCE=CACGGCTTCTACCTGAAGATTT
PRIMER_LEFT_3=601,22
PRIMER_RIGHT_3=713,22
PRIMER_LEFT_3_TM=59.677
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.5742
PRIMER_LEFT_4_PENALTY=0.322824
PRIMER_RIGHT_4_PENALTY=0.251350
PRIMER_LEFT_4_SEQUENCE=GCATTTATTAAGGATCCCGATG
PRIMER_RIGHT_4_SEQUENCE=GCAAACTAATTTGGAGAAAGCG
PRIMER_LEFT_4=601,22
PRIMER_RIGHT_4=889,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=60.251
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_00632 Ps_contig_mira-and-tgicl-ass_franssen_6106
SEQUENCE=GGTTAACAARABTTTATAATAACACTTCTTTTTTTCCAATTTGGATCTCATGCAAGCTAATAAACATGTATAACTTCGCTACAATGAATTATTACATACAGCCAAAGAAGAAAATAAGTTTATGCAAAGATTATGCCTAAAATGATGGATTTCAATATTCTTATGGACTGGTGAAAATGTGTTGAGGTACTACAACTCTGCTGTGAATTTTGTAGAATGAAACTGTCATTCTGAACCAAACCAAACAATCAATTTCAATTCTTATTGCATTAACTTGTGAATTCATATTCGTTGGCGGTATCGGATGCATTCACTCTAACTCAGCTGGAGTTTGCGCAATGACAGAGGTTGATAGTGAGGGTCGTAGGAGAATGTATATAGCAGGACCCAGCAATGGTATCAATGATACTGGAAGAAGCCAAGAACCTTTGTCAAACCATTTTCTAGCTGTCATGTCATTGTAAACCCAAAATGGTGCCAATGTTGAGAGTAATGTAAAATCAATGGACATAATATGGATAAATTTGCTTCCTCTGCTATACTGGTAAAATTCTTTCCATACATCTCCCCCGGCTAAACCGGCGTAAATTATGAGGCCTATCCCCGAAGCAAGCAGTATCGATGCAGTAACTTTTGATTCCAGAAAATTGAGTGGCCATGTTTTAAGCTGAGCTTCTTCGACAGGAGGTGGTGGAGGTTTCCATAGCACAAAATAAGGAAGAAGTGCATATATGCCGCCGAAAAACGAGAGTGACAGGAAAGGCCATACAGGAACCTTGCTTTTTGAGCTTCTTCCGGTGGGAAGTAACAGCATGCTATATACCAAAGGCCACAAGCCCATTAGATACCACTCAGATACAATCACTTCATTCATTCTGAAACCATCACCTCCTTTCAAATTCAACAGCTTTTCCAAGAAATACATGTCCATAGACGGTGTTTGGTTTGGAGAGAGAAAGGAGACATAGTAGATAAGTGCAGCCCATAAGAGAAATAGCAAAATTGAGGTTGTCCAATCTCTTTTTCCTTCCTCCACAACCTCAACAACGCTTCTGCTAACCTCACTCTCTTTGTTGGAATCATGGCAAATAACAATACTACCCTTTTTCCGAAACGAAGCAGCAAAGGGTTTTTTTGGTCATCACCTTGCAGGAGCTACTGAGTCTGATGAAACAACATCGTGAGACCAATGCTGTTGTTGTTGTAGTTGATGTATGTGGTTGAATTGAATACTTGTTTTTGAGAGTTGGAAAGTTGCAAGAGACAAGATTGGGTTTAGCTACTACTCCCACCATAATGTTTTCTCTCATAAGATACACAAACAACATCMATTHCAATGTGATATAGTC
TARGET=1194,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00633 Ps_contig_mira-and-tgicl-ass_franssen_6143
SEQUENCE=GATCACTCTCATACCTAATTCATTWACCGAAAAAAWCCTATTCGTATCCGATTACTCACAAACTCTAAGCTAAGCAAAGCAATGACGCAAGAAGAGAACAATCAACCAACCTTCGAGACGGAGGTGTGTCCCGGGTTTGTTGCAATGGACAAGTCACGGACGGAACCGAACTTGCACAACTTCTGAGAGGTGTTACTTAAACTGTATGAAACTATGCGAAACCGGATCAACTGTAAAACTGTTTGGACTGAGCTACTTGTGCATTGGCTTTGGTTACTTGAAGGAACTGTTGGTATAGGAAAGTTCTAAACCCTGCTACGCCAATTGTCGATGGATGGATGCACGGTATGCCGTAGGTTTGTTAGCTTTCAAATTCTGGTGGAACTATTAATGTAGTTTGGTAGGATTTTGGAGTTAATGGTTGGATTTTAATTATTTGTTAGTG
TARGET=330,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00634 Ps_contig_mira-and-tgicl-ass_franssen_6161
SEQUENCE=AGTTGTGATTTAGCCAAAGGAAAAGCAGAAGATGATGTTTTGTGATTCTGGAAATCATTGTGGATGGTGATGTGAGGCGCGGGAAGAAGAAGAAGATATAGGTGTTGTTTGTTAGCAGAAAAGTGGGGAGAGTGAAAGCTCCGATCTTAGCTCATGAGTTTATATCGAGTTGTTTGCAATCGGATGTGAGAGCTTGAAGTTTGAACTTGGTCGCGTTTTCAATTCATGTTGCATTTCATTTTTGTCATGCTGCATTTTGGCTTGCATTTTGGTTTGTGCTGCAATTTCTTGGCAATGTTGCAATTGCGTTTCGTTTCACTTCATGCTGCATTTCATTTTGTAAATTACTGCGTTTTGGCTTTGCAATGCCTACATTTCCAATTGCCTTTTTTTCCTTTTTTGTCTTATTTGATATAGTACTTGGACCTTGCTTTTGTTGTCTTATTTGGTATGCTTCGATATGGTTACTATAATTTGAATCTTTGATATTGGATATATATAATGGATTTTGTTGGATATAC
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0521
PRIMER_LEFT_PENALTY=0.017127
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=TTAGCCAAAGGAAAAGCAGAAG
PRIMER_RIGHT_SEQUENCE=CCCCACTTTTCTGCTAACAAAC
PRIMER_LEFT=9,22
PRIMER_RIGHT=127,22
PRIMER_LEFT_TM=60.017
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.3004
PRIMER_LEFT_1_PENALTY=0.058624
PRIMER_RIGHT_1_PENALTY=0.241736
PRIMER_LEFT_1_SEQUENCE=GAAATCATTGTGGATGGTGATG
PRIMER_RIGHT_1_SEQUENCE=GAAATGCAGCATGAAGTGAAAC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=60.059
PRIMER_RIGHT_1_TM=59.758
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.3753
PRIMER_LEFT_2_PENALTY=0.017127
PRIMER_RIGHT_2_PENALTY=0.358210
PRIMER_LEFT_2_SEQUENCE=TTAGCCAAAGGAAAAGCAGAAG
PRIMER_RIGHT_2_SEQUENCE=GCTAAGATCGGAGCTTTCACTC
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=60.017
PRIMER_RIGHT_2_TM=59.642
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.3753
PRIMER_LEFT_3_PENALTY=0.017127
PRIMER_RIGHT_3_PENALTY=0.358210
PRIMER_LEFT_3_SEQUENCE=TTAGCCAAAGGAAAAGCAGAAG
PRIMER_RIGHT_3_SEQUENCE=GAGCTAAGATCGGAGCTTTCAC
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=60.017
PRIMER_RIGHT_3_TM=59.642
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.4168
PRIMER_LEFT_4_PENALTY=0.058624
PRIMER_RIGHT_4_PENALTY=0.358210
PRIMER_LEFT_4_SEQUENCE=GAAATCATTGTGGATGGTGATG
PRIMER_RIGHT_4_SEQUENCE=GCTAAGATCGGAGCTTTCACTC
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.642
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00635 Ps_contig_mira-and-tgicl-ass_franssen_6172
SEQUENCE=AAATTAAATTAATGACAGTAACAAAACATCATGTAAAATAAATGGTAAGTAAGTATATTTGGAGTTTGAAATAGGGAAATGGTAGTTACCTGWYTTTAGGTTGTGCCAGATGTTGTTGGCGGTGGCTTCTGCTTTTTTGCTCCAAGAATCGAACATGTGGAGAATTGATTCCATGGGACTGTTGGCAGGTTTTTCGACAGGGGTGTGTTGAATATAAGGATGATGATCATGTTGGTAATACTGAACCTGAGGTTGTTGTTGTTGTTGTGGATCGGTTTGTAAAGCTGCTTTTTTGTTGTCTGGGTGACTACTTGGGACTGCTGGTGCTCCCATCATGTGAGTTCCCCAATTCTCAGATGTAGAAGGAGGTGATGACGGTGATGCAAATGGGTTTTCATCTTGGGATTGGGTTTTGTGATTGTTGTTGTTGTTCATGTTTTTGTATGGGTGAGAAAGAAAAAAAGGTTAGTGATGAGAGATTGAGAAAAAGGATSAAAAAGAAAATAGAAAATGGTTAGATTTAAGGAGAGAAGAA
TARGET=252,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00636 Ps_contig_mira-and-tgicl-ass_franssen_6172
SEQUENCE=AAATTAAATTAATGACAGTAACAAAACATCATGTAAAATAAATGGTAAGTAAGTATATTTGGAGTTTGAAATAGGGAAATGGTAGTTACCTGWYTTTAGGTTGTGCCAGATGTTGTTGGCGGTGGCTTCTGCTTTTTTGCTCCAAGAATCGAACATGTGGAGAATTGATTCCATGGGACTGTTGGCAGGTTTTTCGACAGGGGTGTGTTGAATATAAGGATGATGATCATGTTGGTAATACTGAACCTGAGGTTGTTGTTGTTGTTGTGGATCGGTTTGTAAAGCTGCTTTTTTGTTGTCTGGGTGACTACTTGGGACTGCTGGTGCTCCCATCATGTGAGTTCCCCAATTCTCAGATGTAGAAGGAGGTGATGACGGTGATGCAAATGGGTTTTCATCTTGGGATTGGGTTTTGTGATTGTTGTTGTTGTTCATGTTTTTGTATGGGTGAGAAAGAAAAAAAGGTTAGTGATGAGAGATTGAGAAAAAGGATSAAAAAGAAAATAGAAAATGGTTAGATTTAAGGAGAGAAGAA
TARGET=419,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00637 Ps_contig_mira-and-tgicl-ass_franssen_6181
SEQUENCE=CAAATATGAACAATATAAGAAACCCAAAACAAGGCTTAAGGTAACACATAAACTAACAAAAGACATGGTCTCTTTTCTATCAAAATTTCACATCTTCATTCTATCAAATATACAAAAATAATAATTAAATATCAACTATGAAAAATTTCGGAGTAATTAAGGAGGTAAATGAAGTGATATCCGACAACAAATCTAAGTACCTAGGAGTAAGCTAAAATCAAAACCACAAAAGGATGGACCATGCAACTTCTTCAAATGAAGGACAAAGGTGAATGAATGAATTTCTTCTATATCTTGAAAACTTTAGTTAAGATATTGGTACTATTTTTCTAAGACCTATCTTAACTAATTGTGNTTTTTCTTTCTAAGGGAAAAGTCTTACCTTACAAGTAGAGGGCGATGACTTATGNGTTGTGTGAATAGANAATTTA
TARGET=270,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7670
PRIMER_LEFT_PENALTY=0.360320
PRIMER_RIGHT_PENALTY=0.406645
PRIMER_LEFT_SEQUENCE=GGACCATGCAACTTCTTCAAAT
PRIMER_RIGHT_SEQUENCE=CNCATAAGTCATCGCCCTCTAC
PRIMER_LEFT=235,22
PRIMER_RIGHT=410,22
PRIMER_LEFT_TM=60.360
PRIMER_RIGHT_TM=60.407
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.7670
PRIMER_LEFT_1_PENALTY=0.360320
PRIMER_RIGHT_1_PENALTY=0.406645
PRIMER_LEFT_1_SEQUENCE=GGACCATGCAACTTCTTCAAAT
PRIMER_RIGHT_1_SEQUENCE=ACNCATAAGTCATCGCCCTCTA
PRIMER_LEFT_1=235,22
PRIMER_RIGHT_1=411,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=60.407
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.50
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.7921
PRIMER_LEFT_2_PENALTY=0.385416
PRIMER_RIGHT_2_PENALTY=0.406645
PRIMER_LEFT_2_SEQUENCE=GACCATGCAACTTCTTCAAATG
PRIMER_RIGHT_2_SEQUENCE=CNCATAAGTCATCGCCCTCTAC
PRIMER_LEFT_2=236,22
PRIMER_RIGHT_2=410,22
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=60.407
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.7921
PRIMER_LEFT_3_PENALTY=0.385416
PRIMER_RIGHT_3_PENALTY=0.406645
PRIMER_LEFT_3_SEQUENCE=GACCATGCAACTTCTTCAAATG
PRIMER_RIGHT_3_SEQUENCE=ACNCATAAGTCATCGCCCTCTA
PRIMER_LEFT_3=236,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=59.615
PRIMER_RIGHT_3_TM=60.407
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.50
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=1.2429
PRIMER_LEFT_4_PENALTY=0.360320
PRIMER_RIGHT_4_PENALTY=0.882618
PRIMER_LEFT_4_SEQUENCE=GGACCATGCAACTTCTTCAAAT
PRIMER_RIGHT_4_SEQUENCE=CACACAACNCATAAGTCATCGC
PRIMER_LEFT_4=235,22
PRIMER_RIGHT_4=417,22
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=60.883
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.50
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_00638 Ps_contig_mira-and-tgicl-ass_franssen_6202
SEQUENCE=TTGGGGGAGGGTGAAGAGAAGAAAAGAAAAGGCTGCGAATTTAAAAACGTTTTCTCTGTTTGAGTATATCCACTGAAAAGAGCGCAGCCAGGAGAGAGAACAAAACCCTTTCAAAGGTTTTGAAGATAACAACAATGAATACTTGTTCTTAGACCCAACGGAACAACGTTGTTGTAGTAACAAGTAACCACAAATGCTTCCGCTTAGAAGAAGAAGAACAAGTAGTAACGAATAATAATAATAATGCAGAAACTTCTTCAGCGTCCAACTTGTTCACTCCCAATCCCTATTCTATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCAACCCTCTCTTTCTCCATCTCCCAACAACAATGTCTACAGCAGCAGCAACAACAACGCCGTCACTCCATTAAGTAATTTCATTCCC
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_LEFT=140,22
PRIMER_RIGHT=370,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.074695
PRIMER_LEFT_1_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_1_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.1692
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_2_SEQUENCE=TAGGGATTGGGAGTGAACAAGT
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.2001
PRIMER_LEFT_3_PENALTY=0.125411
PRIMER_RIGHT_3_PENALTY=0.074695
PRIMER_LEFT_3_SEQUENCE=TATATCCACTGAAAAGAGCGCA
PRIMER_RIGHT_3_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2201
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_4_SEQUENCE=GAATAGGGATTGGGAGTGAACA
PRIMER_LEFT_4=140,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_00639 Ps_contig_mira-and-tgicl-ass_franssen_6202
SEQUENCE=TTGGGGGAGGGTGAAGAGAAGAAAAGAAAAGGCTGCGAATTTAAAAACGTTTTCTCTGTTTGAGTATATCCACTGAAAAGAGCGCAGCCAGGAGAGAGAACAAAACCCTTTCAAAGGTTTTGAAGATAACAACAATGAATACTTGTTCTTAGACCCAACGGAACAACGTTGTTGTAGTAACAAGTAACCACAAATGCTTCCGCTTAGAAGAAGAAGAACAAGTAGTAACGAATAATAATAATAATGCAGAAACTTCTTCAGCGTCCAACTTGTTCACTCCCAATCCCTATTCTATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCAACCCTCTCTTTCTCCATCTCCCAACAACAATGTCTACAGCAGCAGCAACAACAACGCCGTCACTCCATTAAGTAATTTCATTCCC
TARGET=231,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_LEFT=140,22
PRIMER_RIGHT=370,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.074695
PRIMER_LEFT_1_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_1_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.1692
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_2_SEQUENCE=TAGGGATTGGGAGTGAACAAGT
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.2001
PRIMER_LEFT_3_PENALTY=0.125411
PRIMER_RIGHT_3_PENALTY=0.074695
PRIMER_LEFT_3_SEQUENCE=TATATCCACTGAAAAGAGCGCA
PRIMER_RIGHT_3_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2201
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_4_SEQUENCE=GAATAGGGATTGGGAGTGAACA
PRIMER_LEFT_4=140,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_