Genetic and gene expression analysis of flowering time regulation by light quality in lentil.

Publication Overview
TitleGenetic and gene expression analysis of flowering time regulation by light quality in lentil.
AuthorsYuan HY, Caron CT, Ramsay L, Fratini R, Pérez de la Vega M, Vandenberg A, Weller JL, Bett KE
TypeJournal Article
Journal NameAnnals of botany
Year2021
CitationYuan HY, Caron CT, Ramsay L, Fratini R, Pérez de la Vega M, Vandenberg A, Weller JL, Bett KE. Genetic and gene expression analysis of flowering time regulation by light quality in lentil.. Annals of botany. 2021 Jun 29.

Abstract

BACKGROUND AND AIMS
Flowering time is important due to its roles in adaptation to different environments and subsequent formation of crop yield. Changes in light quality affect a range of developmental processes including flowering time, however little is known about light quality induced flowering time control in lentil. This study aims to investigate the genetic basis for differences in flowering response to light quality in lentil.

METHODS
We explored variation in flowering time caused by changes in red/far-red related light quality environments of a lentil interspecific recombinant inbred line population developed from a cross between Lens culinaris cv. Lupa and L. orientalis accession BGE 016880. A genetic linkage map was constructed and then used for identifying QTL associated with flowering time regulation under different light quality environments. Differential gene expression analysis through transcriptomic study and RT-qPCR were used to identify potential candidate genes.

KEY RESULTS
QTL mapping located 13 QTLs controlling flower time under different light quality environments, with phenotypic variance explained ranging from 1.7 to 62.9%. Transcriptomic profiling and gene expression analysis for both parents of this interspecific RIL population identified flowering-related genes showing environment-specific differential expression (flowering DEGs). One of these, a member of the florigen gene family FTa1 (LcFTa1) was located close to 3 major QTLs. Furthermore, gene expression results suggests two other florigen genes (LcFTb1 and LcFTb2), MADS-box transcription factors like LcAGL6/13d, LcSVPb, LcSOC1b and LcFULb, as well as bHLH transcription factor LcPIF6 and Gibberellin 20 oxidase LcGA20oxC,G, may be involved in the light quality response as well.

CONCLUSIONS
Our results show that a major component of flowering time sensitivity to light quality is tightly linked to LcFTa1 and associated with changes in its expression. This work provides a foundation for crop improvement of lentil with better adaptation to variable light environments.

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Projects
This publication contains information about 1 projects:
Project NameDescription
Lentil-Flowering_time-Yuan-2021
Featuremaps
This publication contains information about 1 maps:
Map Name
Lentil-Lupa/BGE016680-RIL
Stocks
This publication contains information about 2 stocks:
Stock NameUniquenameType
BGE016680BGE016680accession
Lupa/BGE016680-RILLupa/BGE016680-RILpopulation
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1095-8290
eISSN1095-8290
Publication Date2021 Jun 29
Journal AbbreviationAnn Bot
PIImcab083
Elocation10.1093/aob/mcab083
DOI10.1093/aob/mcab083
Language Abbreng
Publication TypeJournal Article
Journal CountryEngland
Copyright© The Author(s) 2021. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
LanguageEnglish