Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)

Publication Overview
TitleConstruction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)
AuthorsSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S
TypeJournal Article
Journal NameMolecular Breeding
Volume35
Year2015
Page(s)185
CitationSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S. Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.). 2015; 35:185.

Abstract

Field pea (Pisum sativum L.) is a grain legume crop that is cultivated for either human or livestock consumption. Development of varieties adapted to damaging abiotic and biotic stresses is a major objective for field pea breeding. Bacterial blight is a serious disease caused by the pathogenic agents Pseudomonas syringae pv. syringae and Pseudomonas syringae pv. pisi. A recombinant inbred line (RIL) genetic mapping population was generated by crossing the susceptible genotype Kaspa to the resistant genotype PBA Oura. Previously described sets of single-nucleotide polymorphism and simple sequence repeat markers were assigned to a genetic linkage map of the Kaspa × PBA Oura population, which contained 358 markers spanning 1070 cM with an average marker density of 1 locus per 3.0 cM. Combination with multiple previously published maps (including that of Kaspa × Parafield) subsequently generated an integrated structure with much higher marker density of 1 locus per 0.85 cM. The Kaspa × PBA Oura and Kaspa × Parafield RILs were screened at the seedling stage for resistance to both pathovars. Totals of four and two QTLs for resistance to infection by P. syringae pv. syringae were detected in the Kaspa × Parafield and Kaspa × PBA Oura populations, respectively. A single common genomic region associated with P. syringae pv. pisi resistance was identified in both mapping populations. To integrate information on bacterial blight resistance from various QTL mapping studies, the relevant regions were extrapolated on to the integrated map through use of common flanking markers. The resources generated in this study will support map enhancement, identification of marker-trait associations, genomics-assisted breeding, map-based gene isolation and comparative genetics.
Features
This publication contains information about 3,014 features:
Feature NameUniquenameType
Ps000356Ps000356genetic_marker
Ps001039Ps001039genetic_marker
Ps001824Ps001824genetic_marker
Ps001789Ps001789genetic_marker
Ps001733Ps001733genetic_marker
Ps001804Ps001804genetic_marker
Ps001841Ps001841genetic_marker
PsC4586p208PsC4586p208genetic_marker
SNP_100000831SNP_100000831genetic_marker
Ps001706Ps001706genetic_marker
Ps001593Ps001593genetic_marker
Ps001773Ps001773genetic_marker
PsC12754p283PsC12754p283genetic_marker
AB23AB23genetic_marker
SNP_100000107SNP_100000107genetic_marker
SNP_100000067SNP_100000067genetic_marker
SNP_100000099SNP_100000099genetic_marker
SNP_100000736SNP_100000736genetic_marker
SNP_100000092SNP_100000092genetic_marker
PsC9144p234PsC9144p234genetic_marker
PsC8828p175PsC8828p175genetic_marker
SNP_100000199SNP_100000199genetic_marker
SSSSgenetic_marker
PsC14181p251PsC14181p251genetic_marker
Sts1Sts1genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Bacterial_Blight_Resistance-Sudheesh-2015
Featuremaps
This publication contains information about 3 maps:
Map Name
Pea-Kaspa_x_PBA Oura-RIL
Pea-Integrated_Map-Sudheesh-2015
Pea-Composite_Map-Sudheesh-2015
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
KaspaKaspaaccession
PBA OuraPBA Ouraaccession
Kaspa_x_PBA OuraKaspa_x_PBA Ourapopulation
Properties
Additional details for this publication include:
Property NameValue
URLhttp://link.springer.com/article/10.1007/s11032-015-0376-4