Saturation mapping of regions determining resistance to Ascochyta blight and broomrape in faba bean using transcriptome-based SNP genotyping

Publication Overview
TitleSaturation mapping of regions determining resistance to Ascochyta blight and broomrape in faba bean using transcriptome-based SNP genotyping
AuthorsOcaña-Moral S, Gutiérrez N, Torres AM, Madrid E
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2017
CitationOcaña-Moral S, Gutiérrez N, Torres AM, Madrid E. Saturation mapping of regions determining resistance to Ascochyta blight and broomrape in faba bean using transcriptome-based SNP genotyping. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2017 Aug 08.

Abstract

KEY MESSAGE
Transcriptome-based SNP markers were genotyped in a faba bean map to saturate regions bearing QTL for Ascochyta fabae and broomrape and distinguish positional and functional candidates underlying both resistances. Faba bean is an important food crop worldwide. Marker-assisted selection for disease resistance is a top priority in current faba bean research programs, with pathogens such as Ascochyta fabae and broomrape (Orobanche crenata) being among the major constraints in global faba bean production. However, progress in genetics and genomics in this species has lagged behind that of other grain legumes. Although genetic maps are available, most markers are not in or are too distant from target genes to enable an accurate prediction of the desired phenotypes. In this study, a set of SNP markers located in gene coding regions was selected using transcriptomic data. Ninety-two new SNP markers were genotyped to obtain the most complete map reported so far in the 29H × Vf136 faba bean population. Most of the QTL regions previously described in this cross were enriched with SNP markers. Two QTLs for O. crenata resistance (Oc7 and Oc8) were confirmed. Oc7 and Oc10 located nearby a QTL for A. fabae resistance suggested that these genomic regions might encode common resistance mechanisms and could be targets for selection strategies against both pathogens. We also confirmed three regions in chromosomes II (Af2), III (Af3) and VI associated with Ascochyta blight resistance. The QTLs ratified in the present study are now flanked by or include reliable SNP markers in their intervals. This new information provides a valuable starting point in the search for relevant positional and functional candidates underlying both types of resistance.

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Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
FabaBean-Resistance_Ascochyta_Broomrape-Ocana-Moral-2017
Featuremaps
This publication contains information about 1 maps:
Map Name
faba-29H_x_Vf136-RIL-2017
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
29H29Haccession
Vf136Vf136accession
29H_x_Vf13629H_x_Vf136population
Properties
Additional details for this publication include:
Property NameValue
DOI10.1007/s00122-017-2958-5
eISSN1432-2242
Elocation10.1007/s00122-017-2958-5
ISSN1432-2242
Journal AbbreviationTheor. Appl. Genet.
Journal CountryGermany
LanguageEnglish
Language Abbreng
Publication Date2017 Aug 08
Publication ModelPrint-Electronic
Publication TypeJournal Article
URLhttps://link.springer.com/article/10.1007/s00122-017-2958-5