Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil

Publication Overview
TitleConstruction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil
AuthorsPolanco C, Sáenz de Miera LE, González AI, García P, Fratini R, Vaquero F, Vences FJ, Pérez de la Vega M
TypeJournal Article
Journal NamePloS one
Volume14
Issue3
Year2019
Page(s)e0214409
CitationPolanco C, Sáenz de Miera LE, González AI, García P, Fratini R, Vaquero F, Vences FJ, Pérez de la Vega M. Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil. PloS one. 2019; 14(3):e0214409.

Abstract

Usage of high-throughput sequencing approaches allow for the generation and characterization of reference transcriptome datasets that support gene-based marker discovery, which in turn can be used to build genetic maps among other purposes. We have obtained a transcriptome assembly including 49,453 genes for the lentil (Lens culinaris Medik.) cultivar Alpo using RNAseq methodology. This transcriptome was used as reference to obtain 6,306 quality polymorphic markers (SNPs and short indels) analyzing genotype data from a RIL population at F7 generation derived from the interspecific cross between L. culinaris cv. Alpo and L. odemensis accession ILWL235. L. odemensis is a wild species included in the secondary gene pool and can be used as a source for gene introgression in lentil breeding programs. Marker data were used to construct the first genetic interspecific map between these two species. This linkage map has been used to precisely identify regions of the CDC-Redberry lentil draft genome in which the candidate genes for some qualitative traits (seed coat spotting pattern, flower color, and stem pigmentation) could be located. The genome regions corresponding to a significant single quantitative trait locus (QTL) controlling "time to flowering" located in chromosome 6 and three QTLs regulating seed size and positioned in chromosomes 1 and 5 (two QTLs) were also identified. Significant QTLs for Ascochyta blight resistance in lentil were mapped to chromosome 6 in the genome region or close to it where QTLs for Ascochyta blight resistance have previously been reported.

Features
This publication contains information about 6,163 features:
Feature NameUniquenameType
S080139S080139genetic_marker
S030206S030206genetic_marker
S109591S109591genetic_marker
S096125S096125genetic_marker
S065602S065602genetic_marker
S001551S001551genetic_marker
S096147S096147genetic_marker
S001559S001559genetic_marker
S001569S001569genetic_marker
S006344S006344genetic_marker
S001541S001541genetic_marker
S069139S069139genetic_marker
S006352S006352genetic_marker
S069151S069151genetic_marker
S069141S069141genetic_marker
S109257S109257genetic_marker
S088938S088938genetic_marker
S034902S034902genetic_marker
S072225S072225genetic_marker
S060040S060040genetic_marker
S034893S034893genetic_marker
S075443S075443genetic_marker
S080271S080271genetic_marker
S075444S075444genetic_marker
S060033S060033genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Lentil-Ascochyta_disease_severity-Polanco-2019
Featuremaps
This publication contains information about 1 maps:
Map Name
Lentil-Alpo/ILWL235-RIL
Stocks
This publication contains information about 2 stocks:
Stock NameUniquenameType
ILWL-235ILWL-235accession
Alpo_x_ILWL-235Alpo_x_ILWL-235population
Properties
Additional details for this publication include:
Property NameValue
URLhttps://www.ncbi.nlm.nih.gov/pubmed/?term=Construction+of+a+high-density+interspecific+(Lens+culinaris+x+L.+odemensis)+genetic+map+based+on+functional+markers+for+mapping+morphological+and+agronomical+traits%2C+and+QTLs+affecting+resistance+to+Ascochyta+in+lentil
DOI10.1371/journal.pone.0214409
eISSN1932-6203
Elocation10.1371/journal.pone.0214409
ISSN1932-6203
Journal AbbreviationPLoS ONE
Journal CountryUnited States
LanguageEnglish
Language Abbreng
Publication Date2019
Publication ModelElectronic-eCollection
Publication TypeJournal Article