Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil

Publication Overview
TitleConstruction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil
AuthorsPolanco C, Sáenz de Miera LE, González AI, García P, Fratini R, Vaquero F, Vences FJ, Pérez de la Vega M
TypeJournal Article
Journal NamePloS one
Volume14
Issue3
Year2019
Page(s)e0214409
CitationPolanco C, Sáenz de Miera LE, González AI, García P, Fratini R, Vaquero F, Vences FJ, Pérez de la Vega M. Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil. PloS one. 2019; 14(3):e0214409.

Abstract

Usage of high-throughput sequencing approaches allow for the generation and characterization of reference transcriptome datasets that support gene-based marker discovery, which in turn can be used to build genetic maps among other purposes. We have obtained a transcriptome assembly including 49,453 genes for the lentil (Lens culinaris Medik.) cultivar Alpo using RNAseq methodology. This transcriptome was used as reference to obtain 6,306 quality polymorphic markers (SNPs and short indels) analyzing genotype data from a RIL population at F7 generation derived from the interspecific cross between L. culinaris cv. Alpo and L. odemensis accession ILWL235. L. odemensis is a wild species included in the secondary gene pool and can be used as a source for gene introgression in lentil breeding programs. Marker data were used to construct the first genetic interspecific map between these two species. This linkage map has been used to precisely identify regions of the CDC-Redberry lentil draft genome in which the candidate genes for some qualitative traits (seed coat spotting pattern, flower color, and stem pigmentation) could be located. The genome regions corresponding to a significant single quantitative trait locus (QTL) controlling "time to flowering" located in chromosome 6 and three QTLs regulating seed size and positioned in chromosomes 1 and 5 (two QTLs) were also identified. Significant QTLs for Ascochyta blight resistance in lentil were mapped to chromosome 6 in the genome region or close to it where QTLs for Ascochyta blight resistance have previously been reported.

Features
This publication contains information about 6,163 features:
Feature NameUniquenameType
S011625S011625genetic_marker
S071384S071384genetic_marker
S083265S083265genetic_marker
S083266S083266genetic_marker
S104739S104739genetic_marker
S102141S102141genetic_marker
S089846S089846genetic_marker
S099317S099317genetic_marker
S099322S099322genetic_marker
S107817S107817genetic_marker
S046184S046184genetic_marker
S079174S079174genetic_marker
S089845S089845genetic_marker
S080944S080944genetic_marker
S007939S007939genetic_marker
S033820S033820genetic_marker
S054008S054008genetic_marker
S026217S026217genetic_marker
S080947S080947genetic_marker
S033818S033818genetic_marker
S023776S023776genetic_marker
S116562S116562genetic_marker
S116580S116580genetic_marker
S087322S087322genetic_marker
S090689S090689genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Lentil-Ascochyta_disease_severity-Polanco-2019
Featuremaps
This publication contains information about 1 maps:
Map Name
Lentil-Alpo/ILWL235-RIL
Stocks
This publication contains information about 2 stocks:
Stock NameUniquenameType
ILWL-235ILWL-235accession
Alpo_x_ILWL-235Alpo_x_ILWL-235population
Properties
Additional details for this publication include:
Property NameValue
URLhttps://www.ncbi.nlm.nih.gov/pubmed/?term=Construction+of+a+high-density+interspecific+(Lens+culinaris+x+L.+odemensis)+genetic+map+based+on+functional+markers+for+mapping+morphological+and+agronomical+traits%2C+and+QTLs+affecting+resistance+to+Ascochyta+in+lentil
DOI10.1371/journal.pone.0214409
eISSN1932-6203
Elocation10.1371/journal.pone.0214409
ISSN1932-6203
Journal AbbreviationPLoS ONE
Journal CountryUnited States
LanguageEnglish
Language Abbreng
Publication Date2019
Publication ModelElectronic-eCollection
Publication TypeJournal Article