Inheritance of faba bean resistance to Broomrape, genetic diversity and QTL mapping analysis.

Publication Overview
TitleInheritance of faba bean resistance to Broomrape, genetic diversity and QTL mapping analysis.
AuthorsAbd El-Fatah BES, Nassef DMT
TypeJournal Article
Journal NameMolecular biology reports
Year2019
CitationAbd El-Fatah BES, Nassef DMT. Inheritance of faba bean resistance to Broomrape, genetic diversity and QTL mapping analysis.. Molecular biology reports. 2019 Oct 04.

Abstract

Six faba bean parents and their F1 and F2 generations were used in this investigation to study the genetic system controlling resistance of faba bean (Vicia faba L.) to broomrape (Orobanche crenata). Most of the F1 hybrids were tolerant to broomrape. In the F2 generation, the population P5 × P6 (Assiut 125 × Romy 12) gave the highest value of relative yield and tolerance index. Heterosis and inbreeding depression were only positive in number of tillers/plant and seed yield/plant characters. The results indicated that the additive effect was more important than the dominance one (D > H1) only for No. of pods/plant in the F1 generation. Moreover, the narrow-sense heritability was low for most of the studied traits. Three molecular marker systems, namely RAPD, ISSR and SRAP were used for identification and estimation of the genetic diversity among the six faba bean genotypes. The three molecular markers generated DNA unique bands for all genotypes. Only, eight DNA fragments were related to Orobanche tolerance. Clearly and reproducible polymorphic markers were subjected to QTL analysis. The linkage analysis showed that, out of 34 marker loci segregated in the F2 population, 29 (85.29%) were mapped on three linkage groups. QTL analysis using SIM method performed for the 29 markers assigned to LG-1, LG-2 and LG-3 with the eight traits, number of tillers/plant, plant height, number of pods/plant, seed yield/plant, number of broomrape spikes per plant, height of broomrape spikes, relative yield and tolerance index, showing 12 putative QTLs for all traits except number of tillers/plant. From this study, it is clear that P5 × P6 (Assiut 125 × Romy12) population could be considered promising for selection for resistance to broomrape infestation.

Stocks
This publication contains information about 36 stocks:
Stock NameUniquenameType
Assiut_85Assiut_85cultivar
Assiut_62Assiut_62cultivar
Romy_5Romy_5cultivar
Assiut_115Assiut_115cultivar
Assiut_125Assiut_125cultivar
Romy_12Romy_12cultivar
Romy_5/Assiut_125-F2Romy_5/Assiut_125-F2population
Assiut_85/Assiut_62-F1Assiut_85/Assiut_62-F1population
Assiut_85/Romy_5-F1Assiut_85/Romy_5-F1population
Assiut_85/Assiut_115-F1Assiut_85/Assiut_115-F1population
Assiut_85/Assiut_125-F1Assiut_85/Assiut_125-F1population
Assiut_85/Romy_12-F1Assiut_85/Romy_12-F1population
Assiut_62/Romy_5-F1Assiut_62/Romy_5-F1population
Assiut_62/Assiut_115-F1Assiut_62/Assiut_115-F1population
Assiut_62/Assiut_125-F1Assiut_62/Assiut_125-F1population
Assiut_62/Romy_12-F1Assiut_62/Romy_12-F1population
Romy_5/Assiut_115-F1Romy_5/Assiut_115-F1population
Romy_5/Assiut_125-F1Romy_5/Assiut_125-F1population
Romy_5/Romy_12-F1Romy_5/Romy_12-F1population
Assiut_115/Assiut_125-F1Assiut_115/Assiut_125-F1population
Assiut_115/Romy_12-F1Assiut_115/Romy_12-F1population
Assiut_125/Romy_12-F1Assiut_125/Romy_12-F1population
Assiut_85/Assiut_62-F2Assiut_85/Assiut_62-F2population
Assiut_85/Romy_5-F2Assiut_85/Romy_5-F2population
Assiut_85/Assiut_115-F2Assiut_85/Assiut_115-F2population

Pages

Features
This publication contains information about 27 features:
Feature NameUniquenameType
EM1aXME1bEM1aXME1bgenetic_marker
EM1aXME2EM1aXME2genetic_marker
EM1cXME3EM1cXME3genetic_marker
EM1cXME4EM1cXME4genetic_marker
EM2XME3EM2XME3genetic_marker
EM2XME4EM2XME4genetic_marker
HBHBgenetic_marker
HB06HB06genetic_marker
HB08HB08genetic_marker
HB09HB09genetic_marker
HB13HB13genetic_marker
HB14HB14genetic_marker
OPA06OPA06genetic_marker
OPU07OPU07genetic_marker
OPY05OPY05genetic_marker
Broomrape shoot lengthqBSL.Romy5/Assiut125.LG02QTL
Number of broomrape shoots per plantqNBBRS.Romy5/Assiut125.LG03QTL
Plant heightqPLHGT.Romy5/Assiut125.LG01QTL
Plant heightqPLHGT.Romy5/Assiut125.LG02QTL
Pod number per plantqPNB.Romy5/Assiut125.LG01QTL
Pod number per plantqPNB.Romy5/Assiut125.LG01.2QTL
Resistance to crenate broomrapeqRCBR.Romy5/Assiut125.LG01QTL
Resistance to crenate broomrapeqRCBR.Romy5/Assiut125.LG01.2QTL
Resistance to crenate broomrapeqRCBR.Romy5/Assiut125.LG01.3QTL
Relative yieldqRY.Romy5/Assiut125.LG01QTL

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Faba_bean-Broomrape_resistance-ElFatah-2019
Featuremaps
This publication contains information about 1 maps:
Map Name
Faba-Romy5/Assiut125-F2
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1573-4978
eISSN1573-4978
Publication Date2019 Oct 04
Journal AbbreviationMol. Biol. Rep.
DOI10.1007/s11033-019-05101-1
Elocation10.1007/s11033-019-05101-1
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryNetherlands
Cross References
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DatabaseAccession
PMID: PubMedPMID:31584142