Pisum_sativum_v1_Contig2064

Contig Overview
NamePisum_sativum_v1_Contig2064
Unique NamePisum_sativum_v1_Contig2064
Typecontig
OrganismPisum sativum (pea)
Sequence length521
Alignments
The following features are aligned
Feature Name Type LocationAnalysisReference
GH720694ESTGH720694:1..521 -Pisum sativum unigene v1n/a
GH720000ESTGH720000:23..500 +Pisum sativum unigene v1n/a
Unigenes
This contig is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1contig
Analyses
This contig is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot2010-12-28
InterProScan analysis for Pisum sativum unigene v12010-12-29
Relationships

The following EST feature(s) are a part of this contig:

Feature NameUnique NameSpeciesType
GH720694GH720694Pisum sativumEST
GH720000GH720000Pisum sativumEST


Homology
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Match: CHIT2_TULBA (Chitinase 2 OS=Tulipa bakeri PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 7.647e-14
Identity = 51/146 (34.93%), Postives = 75/146 (51.37%), Query Frame = -1 
Query:   39 IQMDFILGFASEHYIDGKG----DGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGV---GTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAP 455
            +   FIL FA + Y  G      +G FNP W+ +  S  +V   K+ +  V+V +S+GG    G    FNP     W+DNA  S+  II+ Y         +DGIDI Y+      + F+ CIGQ++ +LK +  ++F   VSIAP
Sbjct:   25 VDFHFILAFAID-YTSGSSPTPTNGNFNPFWDTNNLSPSQVAAIKRTYNNVKVSVSLGGNSVGGERVFFNPSSVSSWVDNAVSSLTKIIKQYH--------LDGIDIDYEHFKGDPNTFAECIGQLVTRLKKNGVVSF---VSIAP 158
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Match: CHIT1_TULBA (Chitinase 1 OS=Tulipa bakeri PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.795e-13
Identity = 49/146 (33.56%), Postives = 75/146 (51.37%), Query Frame = -1 
Query:   39 IQMDFILGFASEHYIDGKG----DGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGV---GTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAP 455
            +   FIL FA + Y  G      +G F P W+ +  S  +V   K+ H  V+V +S+GG    G    F+P     W++NA  S+  II+ Y         +DGIDI Y+      + F+ CIGQ++ +LK ++ ++F   VSIAP
Sbjct:   51 VDFHFILAFAID-YTSGSSPTPTNGNFKPFWDTNNLSPSQVAAVKRTHSNVKVSLSLGGDSVGGKNVFFSPSSVSSWVENAVSSLTRIIKQYH--------LDGIDIDYEHFKGDPNTFAECIGQLVTRLKKNEVVSF---VSIAP 184
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Match: RUAP_SOYBN (RuBisCO-associated protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.763e-10
Identity = 38/128 (29.69%), Postives = 68/128 (53.12%), Query Frame = -1 
Query:   75 LGFASEHYIDGKGDGRFNPTWEVDPSSIERVKKFKQGHP----EVRVFISIGGVGTEFPF---NPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDK 437
            L  A ++  +   +G+F P W+ +  + E +KKFK+ +      V+V +SIG    +FPF   +   +  W+  A  S++ II+ Y+        +DGID+ Y+ I ++  DF   +G ++R LK +K
Sbjct:   33 LSLARDYDGNNSTNGKFIPYWDTEKVTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYN--------LDGIDVSYEDIAANEADFVNSVGGLVRNLKQNK 152
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41675_VICNA (Narbonin OS=Vicia narbonensis PE=2 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 3.724e-28
Identity = 72/157 (45.86%), Postives = 100/157 (63.69%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   I++  E ++  +ILGFA E Y + GKG G F  +W+V+    E+VK  K+ HPEV+V ISIGG G   PF+P E  VW+ NA +S++ II+ Y DD    N+IDGIDIHY+ I S  + F+  +GQ+I +LK D  L    VVSIAP++
Sbjct:   19 LHDFPTEIINT--ETLEFHYILGFAIESYYESGKGTGTFEESWDVELFGPEKVKNLKRRHPEVKVVISIGGRGVNTPFDPAEENVWVSNAKESLKLIIQKYSDDSG--NLIDGIDIHYEHIRSD-EPFATLMGQLITELKKDDDLNIN-VVSIAPSE 169
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q08884_VICNA (Narbonin OS=Vicia narbonensis PE=1 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 3.724e-28
Identity = 72/157 (45.86%), Postives = 100/157 (63.69%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   I++  E ++  +ILGFA E Y + GKG G F  +W+V+    E+VK  K+ HPEV+V ISIGG G   PF+P E  VW+ NA +S++ II+ Y DD    N+IDGIDIHY+ I S  + F+  +GQ+I +LK D  L    VVSIAP++
Sbjct:   19 LHDFPTEIINT--ETLEFHYILGFAIESYYESGKGTGTFEESWDVELFGPEKVKNLKRRHPEVKVVISIGGRGVNTPFDPAEENVWVSNAKESLKLIIQKYSDDSG--NLIDGIDIHYEHIRSD-EPFATLMGQLITELKKDDDLNIN-VVSIAPSE 169
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q7DLM0_VICNA (Narbonin OS=Vicia narbonensis PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 8.296e-28
Identity = 71/157 (45.22%), Postives = 100/157 (63.69%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   +++  E ++  +ILGFA E Y + GKG G F  +W+V+    E+VK  K+ HPEV+V ISIGG G   PF+P E  VW+ NA +S++ II+ Y DD    N+IDGIDIHY+ I S  + F+  +GQ+I +LK D  L    VVSIAP++
Sbjct:   19 LHDSPTEIVNT--ETLEFHYILGFAIESYYESGKGTGTFEESWDVELFGPEKVKNLKRRHPEVKVVISIGGRGVNTPFDPAEENVWVSNAKESLKLIIQKYSDDSG--NLIDGIDIHYEHIRSD-EPFATLMGQLITELKKDDDLNIN-VVSIAPSE 169
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q08883_9FABA (Narbonin OS=Vicia pannonica PE=3 SV=2)

HSP 1 Score: 125.561 bits (314), Expect = 1.848e-27
Identity = 72/157 (45.86%), Postives = 98/157 (62.42%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   ID+  E ++  FILGFA+E Y + GK  G F  +W+V+    E VK  K  HPEV+V ISI G   + PF+P E  +W+  A KS++ II+ Y ++    N+IDGIDI+Y+ INS  + F  CIGQVIR+LK D  L    VVSIAP++
Sbjct:   19 LHDFPHEIIDT--ENLEFHFILGFATESYYESGKSTGNFEESWDVELFGPENVKNLKTKHPEVKVVISIRGHDDKTPFDPDEENIWVWKAVKSLKQIIKKYRNESG--NMIDGIDINYEHINSDDELFVNCIGQVIRELKKDDDLNID-VVSIAPSE 170
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41680_9FABA (Narbonin OS=Vicia pannonica PE=2 SV=2)

HSP 1 Score: 125.176 bits (313), Expect = 2.414e-27
Identity = 69/162 (42.59%), Postives = 104/162 (64.20%), Query Frame = -1 
Query:   18 IHHIPKHRIDSDVERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQVVESY 500
            +H  P   ID+  E ++  FILGFA+E+Y    KG G F  +W+ +   +E VKK K+ +PEV+V ISIGG G + PF+P E  VW++NA +S++ I + Y ++    ++IDGIDI+Y+ I SS + F+  +GQ+I +LK D  L    VVSIAP++   S+
Sbjct:   19 LHDFPSEIIDT--ENLEFHFILGFATENYNQARKGTGTFEESWDSESFGLENVKKLKERYPEVKVVISIGGRGVQTPFHPAEENVWVENAQESLKQIFQKYSNESG--SMIDGIDINYEHI-SSDEPFARLVGQLITELKKDDDLNIN-VVSIAPSETNSSH 174
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q39888_SOYBN (Putative narbonin OS=Glycine max PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 9.173e-27
Identity = 68/162 (41.98%), Postives = 103/162 (63.58%), Query Frame = -1 
Query:   18 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQVVESY 500
            +H  P   ID+  E ++  FILGFA+E Y + GK  G F  +W+ +   +E VKK K+ +PEV+V ISIGG G + PF+P E  VW++NA +S++ I + Y ++    ++IDGIDI+Y+ I SS + F+  +G +I +LK D  L    VVSIAP++   S+
Sbjct:   19 LHDFPHEIIDT--ENLEFHFILGFATESYYESGKSTGNFEESWDSESFGLENVKKLKERYPEVKVVISIGGRGVQTPFHPAEENVWVENAQESLKQIFQKYSNESG--SMIDGIDINYEHI-SSDEPFARLVGLLITELKKDDDLNIN-VVSIAPSETNSSH 174
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41679_9FABA (Narbonin (Fragment) OS=Vicia pannonica PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 7.765e-26
Identity = 67/162 (41.36%), Postives = 103/162 (63.58%), Query Frame = -1 
Query:   18 IHHIPKHRIDSDVERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQVVESY 500
            +H  P   I +  E ++  FILGFA+E+Y    KG G F  +W+ +   +E VKK K+ +PEV+V ISIGG G + PF+P E  VW++NA +S++ I + Y ++    ++I+GIDI+Y+ I SS + F+  +GQ+I +LK D  L    VVSIAP++   S+
Sbjct:   19 LHDFPSEIIGT--ENLEFHFILGFATENYNQARKGTGTFEESWDSESFGLENVKKSKERYPEVKVVISIGGRGVQTPFHPAEENVWVENAQESLKQIFQKYSNENG--SMIEGIDINYEHI-SSDEPFARLVGQLITELKKDDDLNIN-VVSIAPSETNSSH 174
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41704_VICSA (Putative narbonin-like 2S protein OS=Vicia sativa PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.951e-25
Identity = 67/144 (46.53%), Postives = 93/144 (64.58%), Query Frame = -1 
Query:   33 ERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 461
            E I+  FILGFA+E Y +G KG G F  +W  +    ++VK  K  HPEV+V ISIGG G E PF+P E  +W+ NA KS++ II+ Y ++    N+IDGIDI+Y+ I S  + F   IGQ+I +LK ++ L    VVSIAP++
Sbjct:   29 ETIEFHFILGFATEKYNEGRKGTGNFEESWMDEFFGPDKVKNLKTKHPEVKVVISIGGRGVETPFDPAEQNIWVSNAVKSLKLIIQKYKNESG--NLIDGIDINYEHIKSD-EAFPRLIGQLITELKKERDLNIH-VVSIAPSE 168
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41660_VICFA (Putative narbonin-like 2S protein OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.951e-25
Identity = 67/144 (46.53%), Postives = 93/144 (64.58%), Query Frame = -1 
Query:   33 ERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 461
            E I+  FILGFA+E Y +G KG G F  +W  +    ++VK  K  HPEV+V ISIGG G E PF+P E  +W+ NA KS++ II+ Y ++    N+IDGIDI+Y+ I S  + F   IGQ+I +LK ++ L    VVSIAP++
Sbjct:   29 ETIEFHFILGFATEKYNEGRKGTGNFEESWMDEFFGPDKVKNLKTKHPEVKVVISIGGRGVETPFDPAEQNIWVSNAVKSLKLIIQKYKNESG--NLIDGIDINYEHIKSD-EAFPRLIGQLITELKKERDLNIH-VVSIAPSE 168
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41663_VICFA (Nodulin homologous to narbonin OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.574e-25
Identity = 66/144 (45.83%), Postives = 95/144 (65.97%), Query Frame = -1 
Query:   33 ERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 461
            E  +  FILGFA+E+Y +  KG G F  +W+ +    ++VK  K+ HPEV+V ISIGG G E PF+P E +VW+ NA +S++ II+ Y ++    N+IDGIDI+Y  I S  + F   IGQ+IR+LK ++ L    VVSIAP++
Sbjct:   29 ETTEFHFILGFATENYSESRKGSGNFVESWKDEFFGPDKVKILKRKHPEVKVVISIGGHGLETPFDPAEQIVWVSNAVRSLKLIIQKYKNESG--NLIDGIDINYGNIKSD-EAFPRLIGQLIRELKKERDLNIH-VVSIAPSE 168
The following BLAST results are available for this feature:
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 3
Match NameE-valueIdentityDescription
CHIT2_TULBA7.647e-1434.93Chitinase 2 OS=Tulipa bakeri PE=1 SV=1[more]
CHIT1_TULBA3.795e-1333.56Chitinase 1 OS=Tulipa bakeri PE=1 SV=1[more]
RUAP_SOYBN1.763e-1029.69RuBisCO-associated protein OS=Glycine max PE=2 SV=... [more]
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BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q41675_VICNA3.724e-2845.86Narbonin OS=Vicia narbonensis PE=2 SV=2[more]
Q08884_VICNA3.724e-2845.86Narbonin OS=Vicia narbonensis PE=1 SV=2[more]
Q7DLM0_VICNA8.296e-2845.22Narbonin OS=Vicia narbonensis PE=3 SV=1[more]
Q08883_9FABA1.848e-2745.86Narbonin OS=Vicia pannonica PE=3 SV=2[more]
Q41680_9FABA2.414e-2742.59Narbonin OS=Vicia pannonica PE=2 SV=2[more]
Q39888_SOYBN9.173e-2741.98Putative narbonin OS=Glycine max PE=3 SV=1[more]
Q41679_9FABA7.765e-2641.36Narbonin (Fragment) OS=Vicia pannonica PE=2 SV=1[more]
Q41704_VICSA2.951e-2546.53Putative narbonin-like 2S protein OS=Vicia sativa ... [more]
Q41660_VICFA2.951e-2546.53Putative narbonin-like 2S protein OS=Vicia faba PE... [more]
Q41663_VICFA6.574e-2545.83Nodulin homologous to narbonin OS=Vicia faba PE=2 ... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 7..22
score
IPR0006772S globulinPRINTSPR005512SGLOBULINcoord: 17..36
score: 2.5e-15coord: 59..77
score: 2.5e-15coord: 82..96
score: 2.5
IPR001223Glycoside hydrolase, family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 40..160
score: 7.3
IPR013781Glycoside hydrolase, subgroup, catalytic coreGENE3D3.20.20.80no descriptioncoord: 27..162
score: 3.2
IPR017853Glycoside hydrolase, catalytic coreSUPERFAMILY51445(Trans)glycosidasescoord: 27..161
score: 2.1

Sequences
The following sequences are available for this feature:

contig sequence

>Pisum_sativum_v1_Contig2064 ID=Pisum_sativum_v1_Contig2064; Name=Pisum_sativum_v1_Contig2064; organism=Pisum sativum; type=contig; length=521bp
GCGTGGTCGCGGCCGAGGTACGATTCCACCACTTGTGTTGGAGCAATAGA
TACTACCATTTTGAATGCTAAATGTTTATCAAGTTTGAGTTGTCTTATAA
CTTGTCCAATACAGTAGGAAAAATCATCATGACTTGAATTGATCACATCA
TAATGAATATCAATACCATCAATTATATTTTGATCGCTTTGATCATCGTC
ATAGAGTTCGATGATGTGTTGGATCGATTTTGCAGCATTATCGATCCACA
CCAAATTTTCAACTGGATTGAATGGAAATTCAGTGCCAACACCTCCAATG
CTGATAAACACCCTCACTTCTGGATGACCTTGTTTAAACTTTTTCACCCG
TTCAATACTGGAAGGATCAACCTCCCAAGTGGGGTTGAAACGTCCGTCGC
CTTTTCCATCAATGTAATGCTCGCTGGCAAATCCGAGAATAAAGTCCATT
TGTATTCTCTCGACATCGGAATCGATTCTGTGTTTTGGTATATGATGTAT
GTACCTGCCCGNGGCGGCCGC
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Annotated Terms
The following terms have been associated with this contig:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0043169cation binding
Vocabulary: INTERPRO
TermDefinition
IPR000677Chitinase-like
IPR001223Glyco_hydro18_cat
IPR013781Glyco_hydro_catalytic_dom
IPR017853Glycoside_hydrolase_SF