Pisum_sativum_v1_Contig231

Contig Overview


Name	Pisum_sativum_v1_Contig231
Unique Name	Pisum_sativum_v1_Contig231
Type	contig
Organism	Pisum sativum (pea)
Sequence length	561

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
GH720192	EST	GH720192:26..375 +
FG535819	EST	FG535819:1..561 -

Unigenes

This contig is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	contig

Analyses

This contig is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Relationships

The following EST feature(s) are a part of this contig:


Feature Name	Unique Name	Species	Type
GH720192	GH720192	Pisum sativum	EST
FG535819	FG535819	Pisum sativum	EST

Homology

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK1_BRAJU (Cysteine synthase OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.170e-45
Identity = 84/109 (77.06%), Postives = 93/109 (85.32%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GMKGA+ KAEEILAKTPN Y+LQQFENPANPK+HYETTGPEIWKGTDGK+D FVS           GKYLKEQN N+KL+GVEP+ES +LSGGKPGPHKIQGIGAGF+P
Sbjct:  124 GMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTDGKIDGFVSGIGTGGTITGAGKYLKEQNPNVKLYGVEPIESAILSGGKPGPHKIQGIGAGFIP 232

HSP 2 Score: 67.781 bits (164), Expect = 4.256e-11
Identity = 36/74 (48.65%), Postives = 46/74 (62.16%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     P VL+  LIDEV+Q+SSDE+I+ A+LLALKEGL VGISSG          KRPE   K +   F
Sbjct:  222 KIQGIGAGFIPSVLEVDLIDEVVQVSSDESIDMARLLALKEGLLVGISSGAAAAAAIKLAKRPENAGKLFVAVF 295

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK2_BRAJU (Cysteine synthase OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.405e-45
Identity = 83/109 (76.15%), Postives = 93/109 (85.32%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GMKGA+ KAEEILAKTPN Y+LQQFENPANPK+HYETTGPEIWKGT+GK+D F+S           GKYLKEQN N+KL+GVEPVES +LSGGKPGPHKIQGIGAGF+P
Sbjct:  126 GMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTEGKIDGFISGIGTGGTITGAGKYLKEQNPNVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIP 234

HSP 2 Score: 66.2402 bits (160), Expect = 1.238e-10
Identity = 35/74 (47.30%), Postives = 46/74 (62.16%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     P VL+T LIDEV+Q+SSDE+I+ A+LLA +EGL VGISSG          KRPE   K +   F
Sbjct:  224 KIQGIGAGFIPNVLETNLIDEVVQVSSDESIDMARLLAREEGLLVGISSGAAAAAAIKLAKRPENAGKLFVAVF 297

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK1_ARATH (Cysteine synthase OS=Arabidopsis thaliana GN=OASA1 PE=1 SV=2)

HSP 1 Score: 181.03 bits (458), Expect = 3.405e-45
Identity = 84/109 (77.06%), Postives = 93/109 (85.32%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GMKGA+ KAEEILAKTPN Y+LQQFENPANPK+HYETTGPEIWKGT GK+D FVS           GKYLKEQN+N+KL+GVEPVES +LSGGKPGPHKIQGIGAGF+P
Sbjct:  124 GMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIP 232

HSP 2 Score: 65.0846 bits (157), Expect = 2.759e-10
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     P VL+  LIDEV+Q+SSDE+I+ A+ LALKEGL VGISSG          +RPE   K +   F
Sbjct:  222 KIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIF 295

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK_CITLA (Cysteine synthase OS=Citrullus lanatus PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.906e-45
Identity = 86/112 (76.79%), Postives = 94/112 (83.93%), Query Frame = -3

Query:  224 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVPWCL 559
            GMKGAVQKAEEI AKTPN+YILQQFENPANPK+HYETTGPEIW+G+ GK+DA VS           GKYLKEQN NIKL+GVEPVES +LSGGKPGPHKIQGIGAGF+P  L
Sbjct:  127 GMKGAVQKAEEIKAKTPNSYILQQFENPANPKIHYETTGPEIWRGSGGKIDALVSGIGTGGTVTGAGKYLKEQNPNIKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVL 238

HSP 2 Score: 73.1738 bits (178), Expect = 2.779e-19
Identity = 39/68 (57.35%), Postives = 46/68 (67.65%), Query Frame = -1

Query:   61 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 264
            + +G+     PGVLD  L+DEVIQ+SS+E+IETAKLLALKEGL VGISSG          KRPE   K
Sbjct:  225 KIQGIGAGFIPGVLDVNLLDEVIQVSSEESIETAKLLALKEGLLVGISSGAAAAAAIRIAKRPENAGK 292

HSP 3 Score: 42.3578 bits (98), Expect = 2.779e-19
Identity = 20/27 (74.07%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIV +FPSFGER LS+VLF
Sbjct:  285 KRPENAGKLIVAVFPSFGERYLSTVLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK_SOLTU (Cysteine synthase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 8.386e-44
Identity = 83/112 (74.11%), Postives = 93/112 (83.04%), Query Frame = -3

Query:  224 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVPWCL 559
            GMKGA+ KAEEI AKTPN+YILQQFENPANPK+HYETTGPEIWKG++GKVDA  S           GKYL+EQN N+KL+GVEPVES +LSGGKPGPHKIQGIGAGF+P  L
Sbjct:  127 GMKGAISKAEEIKAKTPNSYILQQFENPANPKIHYETTGPEIWKGSNGKVDALASGIGTGGTITGSGKYLREQNPNVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVL 238

HSP 2 Score: 68.5514 bits (166), Expect = 7.947e-19
Identity = 36/68 (52.94%), Postives = 45/68 (66.18%), Query Frame = -1

Query:   61 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 264
            + +G+     PGVL+  LID+V+Q+SS+E+IE AKLLALKEGL VGISSG          KRPE   K
Sbjct:  225 KIQGIGAGFIPGVLEVNLIDDVVQVSSEESIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGK 292

HSP 3 Score: 45.4394 bits (106), Expect = 7.947e-19
Identity = 23/27 (85.19%), Postives = 24/27 (88.89%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVVIFPSFGER LSSVLF
Sbjct:  285 KRPENAGKLIVVIFPSFGERYLSSVLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK_SPIOL (Cysteine synthase OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 1.430e-43
Identity = 85/112 (75.89%), Postives = 92/112 (82.14%), Query Frame = -3

Query:  224 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVPWCL 559
            GMKGAVQKAEEI  KTPN+YILQQFENPANPKVHYETTGPEIWKGT GK+D FVS           GKYLKEQN ++KL G+EPVES VLSGGKPGPHKIQG+GAGF+P  L
Sbjct:  127 GMKGAVQKAEEIRDKTPNSYILQQFENPANPKVHYETTGPEIWKGTGGKIDIFVSGIGTGGTITGAGKYLKEQNPDVKLIGLEPVESAVLSGGKPGPHKIQGLGAGFIPGVL 238

HSP 2 Score: 71.633 bits (174), Expect = 3.614e-19
Identity = 39/68 (57.35%), Postives = 45/68 (66.18%), Query Frame = -1

Query:   61 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 264
            + +GL     PGVLD  +IDEV+QISS+E+IE AKLLALKEGL VGISSG          KRPE   K
Sbjct:  225 KIQGLGAGFIPGVLDVNIIDEVVQISSEESIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGK 292

HSP 3 Score: 43.5134 bits (101), Expect = 3.614e-19
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIV +FPSFGER LSSVLF
Sbjct:  285 KRPENAGKLIVAVFPSFGERYLSSVLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK_WHEAT (Cysteine synthase OS=Triticum aestivum GN=CYS1 PE=2 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 9.272e-43
Identity = 82/112 (73.21%), Postives = 91/112 (81.25%), Query Frame = -3

Query:  224 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVPWCL 559
            GMKGAVQKAEE+ AKTPN+YILQQFEN ANPK+HYETTGPEIWKGT GK+D  VS           GKYL+EQN NIKL+GVEP ES +L+GGKPGPHKIQGIGAGF+P  L
Sbjct:  128 GMKGAVQKAEELAAKTPNSYILQQFENAANPKIHYETTGPEIWKGTGGKIDGLVSGIGTGGTITGTGKYLQEQNPNIKLYGVEPTESAILNGGKPGPHKIQGIGAGFIPGVL 239

HSP 2 Score: 69.3218 bits (168), Expect = 1.463e-11
Identity = 37/74 (50.00%), Postives = 45/74 (60.81%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     PGVLD  +IDE IQ+SSDE+IE AK LALKEGL VGISSG          +RPE   K +   F
Sbjct:  226 KIQGIGAGFIPGVLDVDIIDETIQVSSDESIEMAKSLALKEGLLVGISSGAAAAAAIKVAQRPENAGKLFVVVF 299

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK_MAIZE (Cysteine synthase OS=Zea mays PE=1 SV=2)

HSP 1 Score: 172.94 bits (437), Expect = 9.272e-43
Identity = 82/112 (73.21%), Postives = 92/112 (82.14%), Query Frame = -3

Query:  224 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVPWCL 559
            GMKGAV+KAEEI AKTPN+YILQQFENPANPK+HYETTGPEIWK T GK+D  VS           G+YL+EQN N+KL+GVEPVES VL+GGKPGPHKIQGIGAGF+P  L
Sbjct:  127 GMKGAVKKAEEIQAKTPNSYILQQFENPANPKIHYETTGPEIWKATAGKIDGLVSGIGTGGTITGTGRYLREQNPNVKLYGVEPVESAVLNGGKPGPHKIQGIGAGFIPGVL 238

HSP 2 Score: 72.4034 bits (176), Expect = 1.728e-12
Identity = 39/74 (52.70%), Postives = 46/74 (62.16%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     PGVLD  L+DE +Q+SSDEAIETAK LALKEGL VGISSG          KRPE   K +   F
Sbjct:  225 KIQGIGAGFIPGVLDVDLLDETLQVSSDEAIETAKALALKEGLLVGISSGAAAAAAVRLAKRPENAGKLFVVVF 298

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK1_ORYSJ (Cysteine synthase OS=Oryza sativa subsp. japonica GN=RCS1 PE=2 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 2.698e-42
Identity = 83/112 (74.11%), Postives = 92/112 (82.14%), Query Frame = -3

Query:  224 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVPWCL 559
            GMKGAVQKAEE+ AKT N++ILQQFENPANPK+HYETTGPEIWKGT GKVD  VS           G+YL+EQN +IK++GVEPVES VLSGGKPGPHKIQGIGAGFVP  L
Sbjct:  124 GMKGAVQKAEELAAKTNNSFILQQFENPANPKIHYETTGPEIWKGTGGKVDGLVSGIGTGGTITGAGRYLREQNPDIKIYGVEPVESAVLSGGKPGPHKIQGIGAGFVPGVL 235

HSP 2 Score: 67.781 bits (164), Expect = 4.256e-11
Identity = 37/74 (50.00%), Postives = 45/74 (60.81%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     PGVLD  LI+E +Q+SSDEAIE AK LALKEGL VGISSG          +RPE   K +   F
Sbjct:  222 KIQGIGAGFVPGVLDVDLINETVQVSSDEAIEMAKALALKEGLLVGISSGAAAAAAVRLAQRPENEGKLFVVVF 295

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Match: CYSK2_ORYSJ (Cysteine synthase OS=Oryza sativa subsp. japonica GN=RCS3 PE=2 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.284e-41
Identity = 80/112 (71.43%), Postives = 89/112 (79.46%), Query Frame = -3

Query:  224 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVPWCL 559
            GMKGA+QKA+E+ AK PN+YILQQFENPANPK+HYETTGPEIWK T GKVD  VS           GKYLKEQN  IK++GVEP ES +LSGG+PGPHKIQGIGAGFVP  L
Sbjct:  127 GMKGAIQKADELAAKMPNSYILQQFENPANPKIHYETTGPEIWKATAGKVDILVSGIGTGGTVTGTGKYLKEQNPEIKIYGVEPTESAILSGGRPGPHKIQGIGAGFVPGVL 238

HSP 2 Score: 67.781 bits (164), Expect = 1.426e-17
Identity = 35/64 (54.69%), Postives = 42/64 (65.62%), Query Frame = -1

Query:   73 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPE 264
            + +G+     PGVLD  L+DEV+Q+SSDEAI  AK LALKEGL VGISSG          +RPE
Sbjct:  225 KIQGIGAGFVPGVLDVNLLDEVVQVSSDEAISMAKQLALKEGLLVGISSGAAAVAAIRVAQRPE 288

HSP 3 Score: 41.9726 bits (97), Expect = 1.426e-17
Identity = 19/24 (79.17%), Postives = 22/24 (91.67%), Query Frame = -2

Query:    3 KNAGKLIVVIFPSFGERDLSSVLF 74
            +N GKL+VV+FPSFGER LSSVLF
Sbjct:  288 ENKGKLVVVVFPSFGERYLSSVLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: D7TUP4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_30.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021870001 PE=4 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.411e-55
Identity = 70/109 (64.22%), Postives = 80/109 (73.39%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G+KG   KA+EIL  TPN Y+L QFENPANPK+HYETTGPEIWKGT GKVDA VS           G +LKE+N  IK++GVEP ES VL+GG PGPHKI GIG G +P
Sbjct:  126 GIKGVFDKADEILKNTPNGYLLNQFENPANPKIHYETTGPEIWKGTGGKVDALVSGIGTGGTATGAGHFLKEKNPKIKVYGVEPCESAVLNGGTPGPHKILGIGTGIIP 234

HSP 2 Score: 68.9366 bits (167), Expect = 3.411e-55
Identity = 38/65 (58.46%), Postives = 43/65 (66.15%), Query Frame = -1

Query:   61 GLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 255
            G+   + P VLD  L+DEV+QISSDEA ETAKLLALKEGL VG+SSG          KRPE   K
Sbjct:  227 GIGTGIIPKVLDVKLLDEVVQISSDEAFETAKLLALKEGLLVGMSSGAAAAAAIKVAKRPENAGK 291

HSP 3 Score: 42.743 bits (99), Expect = 3.411e-55
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLI VIFPSFGER LSS+LF
Sbjct:  284 KRPENAGKLITVIFPSFGERYLSSMLF 310

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: A3RM06_SOYBN (Cysteine synthase OS=Glycine max GN=OAS-TL7 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 1.364e-53
Identity = 64/109 (58.72%), Postives = 85/109 (77.98%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G+ G +QKAEE++AKTP++++L QFENPANP +HYETTGPEIW+ +  K+DA VS           G++L+E+N +IKL+GVEP ESPVL+GG+PG H IQGIGAG +P
Sbjct:  127 GIDGVIQKAEELIAKTPDSFMLNQFENPANPNIHYETTGPEIWRDSGEKIDALVSGIGTGGSIAGAGRFLREKNPDIKLYGVEPAESPVLNGGQPGKHLIQGIGAGIIP 235

HSP 2 Score: 68.5514 bits (166), Expect = 1.364e-53
Identity = 37/66 (56.06%), Postives = 44/66 (66.67%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   + P VLD  L+DEVIQ+SS+EAIET KLLALKEGL +GISSG          KRPE   K
Sbjct:  227 QGIGAGIIPKVLDVNLLDEVIQVSSEEAIETVKLLALKEGLLMGISSGAAAAAAIKVGKRPENAGK 292

HSP 3 Score: 43.1282 bits (100), Expect = 1.364e-53
Identity = 22/27 (81.48%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVVIFPSFGER LSS LF
Sbjct:  285 KRPENAGKLIVVIFPSFGERYLSSPLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: C6TJE5_SOYBN (Cysteine synthase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.776e-53
Identity = 64/109 (58.72%), Postives = 85/109 (77.98%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G+ G +QKAEE+LAKTP++++L QFENPANP +HYETTGPEIW+ + GK+DA V+           G++L+E+N +IKL+GVEP ES VL+GG+PG H IQGIGAG +P
Sbjct:  127 GIDGVIQKAEELLAKTPDSFMLNQFENPANPNIHYETTGPEIWRDSGGKIDALVAGIGTGGSIAGAGRFLREKNPDIKLYGVEPAESAVLNGGQPGKHLIQGIGAGIIP 235

HSP 2 Score: 68.5514 bits (166), Expect = 1.776e-53
Identity = 37/66 (56.06%), Postives = 44/66 (66.67%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   + P VLD   +DEVIQ+SS+EAIETAKLLALKEGL +GISSG          KRPE   K
Sbjct:  227 QGIGAGIIPKVLDVNFLDEVIQVSSEEAIETAKLLALKEGLLMGISSGAAAAAAIKVGKRPENAGK 292

HSP 3 Score: 43.1282 bits (100), Expect = 1.776e-53
Identity = 22/27 (81.48%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVVIFPSFGER LSS LF
Sbjct:  285 KRPENAGKLIVVIFPSFGERYLSSPLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: B9MZI2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_595469 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 2.310e-53
Identity = 68/109 (62.39%), Postives = 83/109 (76.15%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G +G + K++EIL  TPN Y+L+QFENPANP++HYETTGPEIWK + GKVDAFV+           GK+LKE+N  IK++GVEP ES VL+GGKPG H IQGIGAG VP
Sbjct:  133 GFQGVLDKSDEILNNTPNGYMLRQFENPANPQIHYETTGPEIWKDSRGKVDAFVAGIGTGGTVTGAGKFLKEKNPEIKVYGVEPTESAVLNGGKPGSHHIQGIGAGVVP 241

HSP 2 Score: 67.0106 bits (162), Expect = 2.310e-53
Identity = 36/66 (54.55%), Postives = 44/66 (66.67%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   + P VLD  L+DEV+Q+SS+EAI+TAKLLAL EGL VGISSG          KRPE   K
Sbjct:  233 QGIGAGVVPPVLDVDLLDEVVQVSSEEAIDTAKLLALNEGLLVGISSGAAAAAAIKVAKRPENAGK 298

HSP 3 Score: 42.3578 bits (98), Expect = 2.310e-53
Identity = 22/27 (81.48%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVVIFPS GER LSSVLF
Sbjct:  291 KRPENAGKLIVVIFPSAGERYLSSVLF 317

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: B9I8A4_POPTR (Cysteine synthase OS=Populus trichocarpa GN=POPTRDRAFT_570971 PE=3 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 1.461e-52
Identity = 65/107 (60.75%), Postives = 82/107 (76.64%), Query Frame = -3

Query:  233 KGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 553
            +G ++KAEEIL  TPN Y+LQQFENPANPK+HYETTGPEIWK + G++D  V+           G++LKE+ + IK++G+EPVES VLSGGK GPH IQGIGAG +P
Sbjct:  128 EGGLKKAEEILRNTPNGYMLQQFENPANPKIHYETTGPEIWKDSGGEIDILVAGIGTGGTVTGAGRFLKEKKAAIKVYGIEPVESAVLSGGKHGPHLIQGIGAGIIP 234

HSP 2 Score: 67.781 bits (164), Expect = 1.461e-52
Identity = 36/66 (54.55%), Postives = 43/66 (65.15%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   + P VLD  ++DEV Q+SS+EAIET KLLALKEGL VGISSG          KRPE   K
Sbjct:  226 QGIGAGIIPSVLDVDMLDEVFQVSSEEAIETTKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGK 291

HSP 3 Score: 42.743 bits (99), Expect = 1.461e-52
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKL+VVIFPSFGER LSS LF
Sbjct:  284 KRPENAGKLVVVIFPSFGERYLSSKLF 310

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: B9MZI5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_595472 PE=4 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.461e-52
Identity = 68/109 (62.39%), Postives = 81/109 (74.31%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G +G + KA EIL  TP+ Y+L+QFENPANP++HYETTGPEIWK + GKVDAFV+           GK+LKE+N  IK++GVEP ES VL GGKPG H IQGIGAG VP
Sbjct:  122 GFQGVLDKAVEILNNTPDGYMLRQFENPANPQIHYETTGPEIWKDSRGKVDAFVAGIGTGGTVTGAGKFLKEKNPEIKVYGVEPTESAVLHGGKPGSHHIQGIGAGIVP 230

HSP 2 Score: 68.5514 bits (166), Expect = 1.461e-52
Identity = 37/66 (56.06%), Postives = 44/66 (66.67%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   + P VLD  L+DEV+Q+SS+EAIETAKLLAL EGL VGISSG          KRPE   K
Sbjct:  222 QGIGAGIVPPVLDVDLLDEVVQVSSEEAIETAKLLALNEGLLVGISSGAAAAAAIKVAKRPENAGK 287

HSP 3 Score: 40.817 bits (94), Expect = 1.461e-52
Identity = 21/27 (77.78%), Postives = 22/27 (81.48%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLI VIFPS GER LSSVLF
Sbjct:  280 KRPENAGKLITVIFPSAGERYLSSVLF 306

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: B9NJL3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_590257 PE=4 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 1.469e-52
Identity = 68/109 (62.39%), Postives = 81/109 (74.31%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G +G + KA EIL  TP+ Y+L+QFENPANP++HYETTGPEIWK + GKVDAFV+           GK+LKE+N  IK++GVEP ES VL GGKPG H IQGIGAG VP
Sbjct:   58 GFQGVLDKAVEILNNTPDGYMLRQFENPANPQIHYETTGPEIWKDSRGKVDAFVAGIGTGGTVTGAGKFLKEKNPEIKVYGVEPTESAVLHGGKPGSHWIQGIGAGVVP 166

HSP 2 Score: 68.1662 bits (165), Expect = 1.469e-52
Identity = 37/66 (56.06%), Postives = 44/66 (66.67%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   + P VLD  L+DEV+Q+SS+EAIETAKLLAL EGL VGISSG          KRPE   K
Sbjct:  158 QGIGAGVVPPVLDVDLLDEVVQVSSEEAIETAKLLALNEGLLVGISSGAAAAAAIKVAKRPENAGK 223

HSP 3 Score: 42.3578 bits (98), Expect = 1.469e-52
Identity = 22/27 (81.48%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVVIFPS GER LSSVLF
Sbjct:  216 KRPENAGKLIVVIFPSAGERYLSSVLF 242

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: B9RET7_RICCO (Cysteine synthase OS=Ricinus communis GN=RCOM_1428710 PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.474e-52
Identity = 67/106 (63.21%), Postives = 82/106 (77.36%), Query Frame = -3

Query:  233 GAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 550
            GA++KAEEIL K P+ Y+LQQFENPANPK+HYETTGPEIW+ T GKVD  VS           G++LKE+N +IK++G+EP ES VL+G KPGPH IQG+GAG VP
Sbjct:  129 GALEKAEEILNKLPDGYMLQQFENPANPKIHYETTGPEIWRDTSGKVDILVSGIGTGGTATGAGRFLKEKNKDIKVYGIEPEESAVLNGEKPGPHLIQGLGAGIVP 234

HSP 2 Score: 66.2402 bits (160), Expect = 2.474e-52
Identity = 37/66 (56.06%), Postives = 42/66 (63.64%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +GL   + P VLD  L+DEV +IS +EAIET KLLALKEGL VGISSG          KRPE   K
Sbjct:  226 QGLGAGIVPNVLDVELLDEVFKISGEEAIETTKLLALKEGLLVGISSGAAAAAAIRLAKRPENAGK 291

HSP 3 Score: 41.2022 bits (95), Expect = 2.474e-52
Identity = 20/27 (74.07%), Postives = 22/27 (81.48%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIV +FPSFGER LSS LF
Sbjct:  284 KRPENAGKLIVAVFPSFGERYLSSKLF 310

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: A3RM05_SOYBN (Cysteine synthase OS=Glycine max GN=OAS-TL6 PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.646e-51
Identity = 62/109 (56.88%), Postives = 80/109 (73.39%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G    ++KAEEI+  TP +Y+L+Q +NPANPK+HYETTGPEIW+ + GKVDA V+           G++LKE+N N+KL+G+EPVES VLSGG PG H IQGIG G +P
Sbjct:  126 GTDAVLRKAEEIVNNTPGSYMLRQIDNPANPKIHYETTGPEIWRDSGGKVDALVAGVGTGGTITGAGRFLKERNPNVKLYGIEPVESTVLSGGPPGDHLIQGIGNGLIP 234

HSP 2 Score: 70.4774 bits (171), Expect = 2.646e-51
Identity = 40/66 (60.61%), Postives = 45/66 (68.18%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   L P VLD  L+DEVIQISS+EAIETAKLLALKEGL +GISSG          KRPE   K
Sbjct:  226 QGIGNGLIPSVLDFNLLDEVIQISSEEAIETAKLLALKEGLLMGISSGAAAAAAIKLGKRPENAGK 291

HSP 3 Score: 41.5874 bits (96), Expect = 2.646e-51
Identity = 20/27 (74.07%), Postives = 23/27 (85.19%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVV+FPS+GER LSS LF
Sbjct:  284 KRPENAGKLIVVVFPSYGERYLSSPLF 310

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Match: B9RET9_RICCO (Cysteine synthase OS=Ricinus communis GN=RCOM_1428730 PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 6.660e-49
Identity = 62/108 (57.41%), Postives = 81/108 (75.00%), Query Frame = -3

Query:  236 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFV 559
            G    ++K +E+LAKTPN Y+L Q ENPANPK+HYETTGPEIW+ + GK+DA V+           G++LKE++S IK++GVEPVES VL+GG PGPH IQGIG+G +
Sbjct:  127 GFTVTLEKIDEMLAKTPNGYMLHQVENPANPKIHYETTGPEIWRDSAGKIDALVAGVGTGGTVTGTGRFLKEKSSKIKVYGVEPVESAVLNGGNPGPHLIQGIGSGII 234

HSP 2 Score: 63.5438 bits (153), Expect = 6.660e-49
Identity = 36/66 (54.55%), Postives = 43/66 (65.15%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   +   VLD  L+DEV+Q+SS+EAIETAKLLALKEGL VGISSG          KR E   K
Sbjct:  227 QGIGSGIIHNVLDAELLDEVLQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAIKLAKRAENAGK 292

HSP 3 Score: 40.0466 bits (92), Expect = 6.660e-49
Identity = 21/27 (77.78%), Postives = 22/27 (81.48%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVVIFPS GER LSS LF
Sbjct:  285 KRAENAGKLIVVIFPSSGERYLSSPLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT3G04940.1 (| Symbols: ATCYSD1, CYSD1 | cysteine synthase D1 | chr3:1365681-1367508 FORWARD LENGTH=324)

HSP 1 Score: 128.642 bits (322), Expect = 1.020e-48
Identity = 57/109 (52.29%), Postives = 80/109 (73.39%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G++G + KAEEI +K P++ +L+QF+NP+NP+ HY TTGPEIW+ + G+VD  V+           G++LKE+N + K++GVEP ES V+SGGKPG H IQGIGAG +P
Sbjct:  127 GVQGIIDKAEEICSKNPDSIMLEQFKNPSNPQTHYRTTGPEIWRDSAGEVDILVAGVGTGGTLSGSGRFLKEKNKDFKVYGVEPTESAVISGGKPGTHLIQGIGAGLIP 235

HSP 2 Score: 64.6994 bits (156), Expect = 1.020e-48
Identity = 37/66 (56.06%), Postives = 43/66 (65.15%), Query Frame = -1

Query:   61 KGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 258
            +G+   L P  LD  ++DEVIQ++S EAIETAKLLALKEGL VGISSG          KRPE   K
Sbjct:  227 QGIGAGLIPDNLDFNVLDEVIQVTSVEAIETAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGK 292

HSP 3 Score: 38.5058 bits (88), Expect = 1.020e-48
Identity = 20/27 (74.07%), Postives = 22/27 (81.48%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +NAGKLIVVIFPS GER LS+ LF
Sbjct:  285 KRPENAGKLIVVIFPSGGERYLSTSLF 311

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT5G28030.1 (| Symbols: DES1 | L-cysteine desulfhydrase 1 | chr5:10030493-10032285 REVERSE LENGTH=323)

HSP 1 Score: 130.954 bits (328), Expect = 1.087e-47
Identity = 63/109 (57.80%), Postives = 79/109 (72.48%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G+KG ++KA+EIL+KTP  YI  QF NP NP++HY TTGPEIW+ + GKVD  V+           GK+LKE+N +IK+  VEP ES VLSGGKPGPH IQGIG+G +P
Sbjct:  126 GIKGQLEKAKEILSKTPGGYIPHQFINPENPEIHYRTTGPEIWRDSAGKVDILVAGVGTGGTVTGTGKFLKEKNKDIKVCVVEPSESAVLSGGKPGPHLIQGIGSGEIP 234

HSP 2 Score: 60.4622 bits (145), Expect = 1.087e-47
Identity = 31/58 (53.45%), Postives = 38/58 (65.52%), Query Frame = -1

Query:   61 PGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 234
            P  LD  ++DE+IQ++ +EAIET KLLA+KEGL VGISSG          KRPE   K
Sbjct:  234 PANLDLSIVDEIIQVTGEEAIETTKLLAIKEGLLVGISSGASAAAALKVAKRPENVGK 291

HSP 3 Score: 36.965 bits (84), Expect = 1.087e-47
Identity = 19/27 (70.37%), Postives = 21/27 (77.78%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +N GKLIVVIFPS GER LS+ LF
Sbjct:  284 KRPENVGKLIVVIFPSGGERYLSTELF 310

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT5G28030.2 (| Symbols: DES1 | L-cysteine desulfhydrase 1 | chr5:10030493-10032285 REVERSE LENGTH=323)

HSP 1 Score: 130.954 bits (328), Expect = 1.087e-47
Identity = 63/109 (57.80%), Postives = 79/109 (72.48%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            G+KG ++KA+EIL+KTP  YI  QF NP NP++HY TTGPEIW+ + GKVD  V+           GK+LKE+N +IK+  VEP ES VLSGGKPGPH IQGIG+G +P
Sbjct:  126 GIKGQLEKAKEILSKTPGGYIPHQFINPENPEIHYRTTGPEIWRDSAGKVDILVAGVGTGGTVTGTGKFLKEKNKDIKVCVVEPSESAVLSGGKPGPHLIQGIGSGEIP 234

HSP 2 Score: 60.4622 bits (145), Expect = 1.087e-47
Identity = 31/58 (53.45%), Postives = 38/58 (65.52%), Query Frame = -1

Query:   61 PGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWK 234
            P  LD  ++DE+IQ++ +EAIET KLLA+KEGL VGISSG          KRPE   K
Sbjct:  234 PANLDLSIVDEIIQVTGEEAIETTKLLAIKEGLLVGISSGASAAAALKVAKRPENVGK 291

HSP 3 Score: 36.965 bits (84), Expect = 1.087e-47
Identity = 19/27 (70.37%), Postives = 21/27 (77.78%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +N GKLIVVIFPS GER LS+ LF
Sbjct:  284 KRPENVGKLIVVIFPSGGERYLSTELF 310

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT4G14880.4 (| Symbols: OASA1 | O-acetylserine (thiol) lyase (OAS-TL) isoform A1 | chr4:8518209-8520050 REVERSE LENGTH=322)

HSP 1 Score: 181.03 bits (458), Expect = 3.645e-46
Identity = 84/109 (77.06%), Postives = 93/109 (85.32%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GMKGA+ KAEEILAKTPN Y+LQQFENPANPK+HYETTGPEIWKGT GK+D FVS           GKYLKEQN+N+KL+GVEPVES +LSGGKPGPHKIQGIGAGF+P
Sbjct:  124 GMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIP 232

HSP 2 Score: 65.0846 bits (157), Expect = 2.947e-11
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     P VL+  LIDEV+Q+SSDE+I+ A+ LALKEGL VGISSG          +RPE   K +   F
Sbjct:  222 KIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIF 295

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT4G14880.3 (| Symbols: OASA1 | O-acetylserine (thiol) lyase (OAS-TL) isoform A1 | chr4:8518209-8520050 REVERSE LENGTH=322)

HSP 1 Score: 181.03 bits (458), Expect = 3.645e-46
Identity = 84/109 (77.06%), Postives = 93/109 (85.32%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GMKGA+ KAEEILAKTPN Y+LQQFENPANPK+HYETTGPEIWKGT GK+D FVS           GKYLKEQN+N+KL+GVEPVES +LSGGKPGPHKIQGIGAGF+P
Sbjct:  124 GMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIP 232

HSP 2 Score: 65.0846 bits (157), Expect = 2.947e-11
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     P VL+  LIDEV+Q+SSDE+I+ A+ LALKEGL VGISSG          +RPE   K +   F
Sbjct:  222 KIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIF 295

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT4G14880.2 (| Symbols: OASA1, OLD3, CYTACS1, ATCYS-3A | O-acetylserine (thiol) lyase (OAS-TL) isoform A1 | chr4:8518209-8520050 REVERSE LENGTH=322)

HSP 1 Score: 181.03 bits (458), Expect = 3.645e-46
Identity = 84/109 (77.06%), Postives = 93/109 (85.32%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GMKGA+ KAEEILAKTPN Y+LQQFENPANPK+HYETTGPEIWKGT GK+D FVS           GKYLKEQN+N+KL+GVEPVES +LSGGKPGPHKIQGIGAGF+P
Sbjct:  124 GMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIP 232

HSP 2 Score: 65.0846 bits (157), Expect = 2.947e-11
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     P VL+  LIDEV+Q+SSDE+I+ A+ LALKEGL VGISSG          +RPE   K +   F
Sbjct:  222 KIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIF 295

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT4G14880.1 (| Symbols: OASA1, OLD3, CYTACS1 | O-acetylserine (thiol) lyase (OAS-TL) isoform A1 | chr4:8518209-8520050 REVERSE LENGTH=322)

HSP 1 Score: 181.03 bits (458), Expect = 3.645e-46
Identity = 84/109 (77.06%), Postives = 93/109 (85.32%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GMKGA+ KAEEILAKTPN Y+LQQFENPANPK+HYETTGPEIWKGT GK+D FVS           GKYLKEQN+N+KL+GVEPVES +LSGGKPGPHKIQGIGAGF+P
Sbjct:  124 GMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIP 232

HSP 2 Score: 65.0846 bits (157), Expect = 2.947e-11
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = -1

Query:   43 RFKGLVLALYPGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSGXXXXXXXXXXKRPEKCWKAYCCHF 264
            + +G+     P VL+  LIDEV+Q+SSDE+I+ A+ LALKEGL VGISSG          +RPE   K +   F
Sbjct:  222 KIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIF 295

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT3G61440.1 (| Symbols: ATCYSC1, ARATH;BSAS3;1, CYSC1 | cysteine synthase C1 | chr3:22735885-22737792 FORWARD LENGTH=368)

HSP 1 Score: 126.331 bits (316), Expect = 1.921e-42
Identity = 58/109 (53.21%), Postives = 76/109 (69.72%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GM G V+KA ++L  TP+A++ QQF NPAN ++H++TTGPEIW+ T G VD FV            G+YLK +N N+K++GVEP ES +L+GGKPGPH I G G GF P
Sbjct:  168 GMGGTVKKAYDLLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKP 276

HSP 2 Score: 48.521 bits (114), Expect = 1.921e-42
Identity = 22/40 (55.00%), Postives = 33/40 (82.50%), Query Frame = -1

Query:  115 PGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSG 234
            P +LD  +++ V+++SS++AI+ A+ LALKEGL VGISSG
Sbjct:  276 PEILDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSG 315

HSP 3 Score: 35.8094 bits (81), Expect = 1.921e-42
Identity = 19/27 (70.37%), Postives = 20/27 (74.07%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +N GKLIV I  SFGER LSSVLF
Sbjct:  326 KMPENKGKLIVTIHASFGERYLSSVLF 352

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT3G61440.2 (| Symbols: ATCYSC1, CYSC1 | cysteine synthase C1 | chr3:22736383-22737792 FORWARD LENGTH=272)

HSP 1 Score: 126.331 bits (316), Expect = 1.935e-42
Identity = 58/109 (53.21%), Postives = 76/109 (69.72%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GM G V+KA ++L  TP+A++ QQF NPAN ++H++TTGPEIW+ T G VD FV            G+YLK +N N+K++GVEP ES +L+GGKPGPH I G G GF P
Sbjct:   72 GMGGTVKKAYDLLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKP 180

HSP 2 Score: 48.521 bits (114), Expect = 1.935e-42
Identity = 22/40 (55.00%), Postives = 33/40 (82.50%), Query Frame = -1

Query:  115 PGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSG 234
            P +LD  +++ V+++SS++AI+ A+ LALKEGL VGISSG
Sbjct:  180 PEILDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSG 219

HSP 3 Score: 35.8094 bits (81), Expect = 1.935e-42
Identity = 19/27 (70.37%), Postives = 20/27 (74.07%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +N GKLIV I  SFGER LSSVLF
Sbjct:  230 KMPENKGKLIVTIHASFGERYLSSVLF 256

[Close]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Match: AT3G61440.3 (| Symbols: CYSC1 | cysteine synthase C1 | chr3:22736550-22737792 FORWARD LENGTH=247)

HSP 1 Score: 126.331 bits (316), Expect = 1.940e-42
Identity = 58/109 (53.21%), Postives = 76/109 (69.72%), Query Frame = -3

Query:  233 GMKGAVQKAEEILAKTPNAYILQQFENPANPKVHYETTGPEIWKGTDGKVDAFVSXXXXXXXXXXXGKYLKEQNSNIKLFGVEPVESPVLSGGKPGPHKIQGIGAGFVP 559
            GM G V+KA ++L  TP+A++ QQF NPAN ++H++TTGPEIW+ T G VD FV            G+YLK +N N+K++GVEP ES +L+GGKPGPH I G G GF P
Sbjct:   47 GMGGTVKKAYDLLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKP 155

HSP 2 Score: 48.521 bits (114), Expect = 1.940e-42
Identity = 22/40 (55.00%), Postives = 33/40 (82.50%), Query Frame = -1

Query:  115 PGVLDTGLIDEVIQISSDEAIETAKLLALKEGLFVGISSG 234
            P +LD  +++ V+++SS++AI+ A+ LALKEGL VGISSG
Sbjct:  155 PEILDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSG 194

HSP 3 Score: 35.8094 bits (81), Expect = 1.940e-42
Identity = 19/27 (70.37%), Postives = 20/27 (74.07%), Query Frame = -2

Query:    3 KGQKNAGKLIVVIFPSFGERDLSSVLF 83
            K  +N GKLIV I  SFGER LSSVLF
Sbjct:  205 KMPENKGKLIVTIHASFGERYLSSVLF 231

The following BLAST results are available for this feature:

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide

BLAST of Pisum_sativum_v1_Contig231 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CYSK1_BRAJU	1.170e-45	77.06	Cysteine synthase OS=Brassica juncea PE=2 SV=1	[more]
CYSK2_BRAJU	3.405e-45	76.15	Cysteine synthase OS=Brassica juncea PE=2 SV=1	[more]
CYSK1_ARATH	3.405e-45	77.06	Cysteine synthase OS=Arabidopsis thaliana GN=OASA1...	[more]
CYSK_CITLA	9.906e-45	76.79	Cysteine synthase OS=Citrullus lanatus PE=2 SV=1	[more]
CYSK_SOLTU	8.386e-44	74.11	Cysteine synthase OS=Solanum tuberosum PE=2 SV=1	[more]
CYSK_SPIOL	1.430e-43	75.89	Cysteine synthase OS=Spinacia oleracea PE=1 SV=1	[more]
CYSK_WHEAT	9.272e-43	73.21	Cysteine synthase OS=Triticum aestivum GN=CYS1 PE=...	[more]
CYSK_MAIZE	9.272e-43	73.21	Cysteine synthase OS=Zea mays PE=1 SV=2	[more]
CYSK1_ORYSJ	2.698e-42	74.11	Cysteine synthase OS=Oryza sativa subsp. japonica ...	[more]
CYSK2_ORYSJ	2.284e-41	71.43	Cysteine synthase OS=Oryza sativa subsp. japonica ...	[more]

BLAST of Pisum_sativum_v1_Contig231 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TUP4_VITVI	3.411e-55	64.22	Whole genome shotgun sequence of line PN40024, sca...	[more]
A3RM06_SOYBN	1.364e-53	58.72	Cysteine synthase OS=Glycine max GN=OAS-TL7 PE=2 S...	[more]
C6TJE5_SOYBN	1.776e-53	58.72	Cysteine synthase OS=Glycine max PE=2 SV=1	[more]
B9MZI2_POPTR	2.310e-53	62.39	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9I8A4_POPTR	1.461e-52	60.75	Cysteine synthase OS=Populus trichocarpa GN=POPTRD...	[more]
B9MZI5_POPTR	1.461e-52	62.39	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9NJL3_POPTR	1.469e-52	62.39	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RET7_RICCO	2.474e-52	63.21	Cysteine synthase OS=Ricinus communis GN=RCOM_1428...	[more]
A3RM05_SOYBN	2.646e-51	56.88	Cysteine synthase OS=Glycine max GN=OAS-TL6 PE=2 S...	[more]
B9RET9_RICCO	6.660e-49	57.41	Cysteine synthase OS=Ricinus communis GN=RCOM_1428...	[more]

BLAST of Pisum_sativum_v1_Contig231 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G04940.1	1.020e-48	52.29	\| Symbols: ATCYSD1, CYSD1 \| cysteine synthase D1 \|...	[more]
AT5G28030.1	1.087e-47	57.80	\| Symbols: DES1 \| L-cysteine desulfhydrase 1 \| chr...	[more]
AT5G28030.2	1.087e-47	57.80	\| Symbols: DES1 \| L-cysteine desulfhydrase 1 \| chr...	[more]
AT4G14880.4	3.645e-46	77.06	\| Symbols: OASA1 \| O-acetylserine (thiol) lyase (O...	[more]
AT4G14880.3	3.645e-46	77.06	\| Symbols: OASA1 \| O-acetylserine (thiol) lyase (O...	[more]
AT4G14880.2	3.645e-46	77.06	\| Symbols: OASA1, OLD3, CYTACS1, ATCYS-3A \| O-acet...	[more]
AT4G14880.1	3.645e-46	77.06	\| Symbols: OASA1, OLD3, CYTACS1 \| O-acetylserine (...	[more]
AT3G61440.1	1.921e-42	53.21	\| Symbols: ATCYSC1, ARATH;BSAS3;1, CYSC1 \| cystein...	[more]
AT3G61440.2	1.935e-42	53.21	\| Symbols: ATCYSC1, CYSC1 \| cysteine synthase C1 \|...	[more]
AT3G61440.3	1.940e-42	53.21	\| Symbols: CYSC1 \| cysteine synthase C1 \| chr3:227...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 31..42 score
None	No IPR available	GENE3D	3.40.50.1100	no description	coord: 21..86 score: 9.6
None	No IPR available	PANTHER	PTHR10314	SER/THR DEHYDRATASE, TRP SYNTHASE	coord: 32..86 score: 1.2
None	No IPR available	PANTHER	PTHR10314:SF8	CYSTEINE SYNTHASE	coord: 32..86 score: 1.2
None	No IPR available	SEG	seg	seg	coord: 72..82 score
None	No IPR available	GENE3D	3.40.50.1100	no description	coord: 8..112 score: 9.3
None	No IPR available	PANTHER	PTHR10314	SER/THR DEHYDRATASE, TRP SYNTHASE	coord: 1..109 score: 8.2
None	No IPR available	PANTHER	PTHR10314:SF8	CYSTEINE SYNTHASE	coord: 1..109 score: 8.2
None	No IPR available	SEG	seg	seg	coord: 56..67 score
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	PFAM	PF00291	PALP	coord: 22..83 score: 4.5
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 16..85 score: 1.3
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	PFAM	PF00291	PALP	coord: 7..107 score: 1.2
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 1..112 score: 8.9

Sequences

The following sequences are available for this feature:

contig sequence

contig sequence

>Pisum_sativum_v1_Contig231 ID=Pisum_sativum_v1_Contig231; Name=Pisum_sativum_v1_Contig231; organism=Pisum sativum; type=contig; length=561bp
TCAAATAGCACAGAGGATAGGTCCCTCTCCCCAAAGCTTGGAAAAATGAC
AACAATAAGCTTTCCAGCATTTTTCTGGCCTTTTGGCTATCTTAATGGCA
GCGGCGGCTGCAGCTCCAGAAGATATTCCCACAAATAGGCCTTCTTTAAG
TGCAAGAAGCTTTGCAGTTTCTATTGCTTCATCACTTGATATTTGAATGA
CTTCATCAATAAGACCAGTGTCCAAGACACCAGGGTACAAAGCCAGCACC
AATCCCTTGAATCTTGTGAGGACCCGGCTTTCCCCCTGAGAGCACCGGAC
TCTCCACCGGTTCCACGCCAAACAGCTTTATATTAGAATTTTGCTCCTTG
AGATATTTCCCAGCACCTGTTATGGTACCACCAGTACCAATCCCAGAAAC
AAATGCGTCAACTTTCCCATCTGTGCCTTTCCATATCTCCGGACCCGTGG
TTTCATAATGCACCTTGGGATTAGCAGGGTTTTCAAATTGCTGAAGTATG
TAAGCATTAGGTGTCTTAGCCAATATCTCTTCCGCTTTCTGAACAGCTCC
TTTCATGCCCT

Annotated Terms

The following terms have been associated with this contig:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001926	TrpB-like_PALP

Search form

Pisum_sativum_v1_Contig231