Pisum_sativum_v1_Contig3056

Contig Overview
NamePisum_sativum_v1_Contig3056
Unique NamePisum_sativum_v1_Contig3056
Typecontig
OrganismPisum sativum (pea)
Sequence length450
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CD858588ESTCD858588:1..448 +
CD859385ESTCD859385:23..450 +
Unigenes
This contig is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1contig
Analyses
This contig is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Relationships

The following EST feature(s) are a part of this contig:

Feature NameUnique NameSpeciesType
CD858588CD858588Pisum sativumEST
CD859385CD859385Pisum sativumEST


Homology
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY2_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 145.206 bits (365), Expect = 1.191e-34
Identity = 67/131 (51.15%), Postives = 93/131 (70.99%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            WWP+++ TF+DNHDTGSTQ  WPFPSD VM+GYAYILTHPG P IFYD+F+DW   + E+I +L+  R R GIH+ S ++I+EA  ++Y A I  K+ +K+G     G+  P G  + ++  G++YAVW K
Sbjct:  300 WWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDW--GLKEEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGG--FKVAAHGNDYAVWEK 426
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY6_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.6 PE=2 SV=2)

HSP 1 Score: 144.821 bits (364), Expect = 1.555e-34
Identity = 69/131 (52.67%), Postives = 92/131 (70.23%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            WWP+++ TF+DNHDTGSTQ  WPFPSD VM+GYAYILTHPG P IFYD+F+DW   + E+I +L+  R RQGIHS S ++I+EA  ++Y A I  K+ +K+G     G   P G  + +   G++YAVW K
Sbjct:  302 WWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPGNPCIFYDHFFDW--GLKEEIDRLVSIRTRQGIHSESKLQIMEADADLYLAEIEGKVIVKLGPRYDVGHLIPEG--FKVVAHGNDYAVWEK 428
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY1_HORVU (Alpha-amylase type A isozyme OS=Hordeum vulgare GN=AMY1.1 PE=1 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.653e-34
Identity = 70/131 (53.44%), Postives = 92/131 (70.23%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            WWP+++ATF+DNHDTGSTQA WPFPSD VM+GYAYILTHPGIP IFYD+F++W     +QI  L+  RKR GI + S ++IL  + + Y A I  K+ +KIG     G+  P+G  +  S  G++YAVW K
Sbjct:  302 WWPAKAATFVDNHDTGSTQAMWPFPSDKVMQGYAYILTHPGIPCIFYDHFFNWGFK--DQIAALVAIRKRNGITATSALKILMHEGDAYVAEIDGKVVVKIGSRYDVGAVIPAG--FVTSAHGNDYAVWEK 428
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY4_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 2.246e-33
Identity = 67/131 (51.15%), Postives = 90/131 (68.70%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            WWP+++ TF+DNHDTGSTQ  WPFPSD VM+GYAYILTHP  P IFYD+F+DW     E+I +L+  R R GIH+ S ++I+EA  ++Y A I  K+ +K+G     G+  P G E   +  G++YAVW K
Sbjct:   26 WWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPRTPCIFYDHFFDWGPK--EEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFEG--AAHGNDYAVWEK 152
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY1_ORYSJ (Alpha-amylase OS=Oryza sativa subsp. japonica GN=AMY1.1 PE=2 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 3.830e-33
Identity = 68/131 (51.91%), Postives = 87/131 (66.41%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            WWP+++ TF+DNHDTGSTQ  WPFPSD VM+GYAYILTHPG P IFYD+F+DW   + E+I +L+  R RQGIH  S +RI+EA  ++Y A I  K+  KIG         P G  + +   G  YA+W K
Sbjct:  307 WWPAKATTFVDNHDTGSTQHLWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDW--GLKEEIERLVSIRNRQGIHPASELRIMEADSDLYLAEIDGKVITKIGPRYDVEHLIPEG--FQVVAHGDGYAIWEK 433
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY3C_ORYSJ (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 137.502 bits (345), Expect = 2.483e-32
Identity = 68/131 (51.91%), Postives = 87/131 (66.41%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            W P ++ TFIDNHDTGSTQ  WPFPSD VM+GYAYILTHPG+P IFYD+ +DWN  + ++I  L   R R GIH  S + IL A  +VY A+I +K+  KIG     G+  PS  ++ +   G+NY VW K
Sbjct:  300 WLPEKAVTFIDNHDTGSTQNSWPFPSDKVMQGYAYILTHPGVPCIFYDHVFDWN--LKQEISTLAAVRSRNGIHPGSKLNILAADGDVYVAMIDDKVITKIGTRYDVGNLIPS--DFHVVAHGNNYCVWEK 426
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY2A_ORYSJ (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. japonica GN=AMY2A PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.483e-32
Identity = 67/131 (51.15%), Postives = 87/131 (66.41%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            WWP+++ TF+DNHDTGSTQ  WPFPSD VM+GYAYILTHPG P IFYD+F+DW   + EQI  L+  R+R G+ + S ++I+    + Y A I  K+ MKIG      S  P G  + L+  G+ YAVW K
Sbjct:  301 WWPAKAVTFVDNHDTGSTQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDW--GLKEQIAALVAVRQRNGVTATSSLKIMLHDADAYVAEIDGKVVMKIGSRYDVSSLIPPG--FHLAAHGNGYAVWEK 427
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY2A_ORYSI (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica GN=AMYC2 PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.483e-32
Identity = 67/131 (51.15%), Postives = 87/131 (66.41%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            WWP+++ TF+DNHDTGSTQ  WPFPSD VM+GYAYILTHPG P IFYD+F+DW   + EQI  L+  R+R G+ + S ++I+    + Y A I  K+ MKIG      S  P G  + L+  G+ YAVW K
Sbjct:  301 WWPAKAVTFVDNHDTGSTQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDW--GLKEQIAALVAVRQRNGVTATSSLKIMLHDADAYVAEIDGKVVMKIGSRYDVSSLIPPG--FHLAAHGNGYAVWEK 427
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY3_WHEAT (Alpha-amylase AMY3 OS=Triticum aestivum GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 4.235e-32
Identity = 68/132 (51.52%), Postives = 87/132 (65.91%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD-----GSWSPSGKEWTLSTSGHNYAVWHK 448
            W P ++ TFIDNHDTGSTQ  WPFPSD VM+GYAYILTHPGIP IFYD+ +DW   + ++I  L   R R GIH  S + IL+A+ ++Y A IG K+  KIG       +  PSG  + ++  G+NY VW K
Sbjct:  283 WMPEKTVTFIDNHDTGSTQRLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDW--KLKQEITALATVRSRNGIHPGSTLDILKAEGDLYVAKIGGKVITKIGSRYNIGDNVIPSG--FKIAAKGNNYCVWEK 410
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Match: AMY3E_ORYSJ (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 5.531e-32
Identity = 65/131 (49.62%), Postives = 89/131 (67.94%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 448
            W P ++ TF+DNHDTGSTQ+ WPFPSD VM+GYAYILTHPGIP IFYD+ +DWN  +  +I  L + R R GIH+ S + IL+A+ ++Y A+I  K+  K+G     G   PS  ++ +   G++Y VW K
Sbjct:  299 WMPDQAVTFVDNHDTGSTQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWN--LQHEIATLAEIRSRNGIHAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPS--DFHVVAHGNDYCVWEK 425
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: C6TCX9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 3.765e-64
Identity = 105/127 (82.68%), Postives = 118/127 (92.91%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSRS TF+DNHDTGSTQAHWPFP DH+MEGYAYILTHPGIPT+FYD+FYDW  S+ EQIVKLID RKRQGI SRS VRILEAKH++Y+A+IGEK+CMKIG+GSW P+G+EWTLSTSGHNYAVWHK
Sbjct:  287 WWPSRSVTFVDNHDTGSTQAHWPFPKDHIMEGYAYILTHPGIPTVFYDHFYDWGDSIREQIVKLIDVRKRQGIQSRSSVRILEAKHDLYSAVIGEKVCMKIGNGSWCPTGREWTLSTSGHNYAVWHK 413
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: B9R8H8_RICCO (Alpha-amylase, putative OS=Ricinus communis GN=RCOM_1599860 PE=3 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 6.011e-62
Identity = 103/127 (81.10%), Postives = 117/127 (92.13%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TFIDNHDTGSTQ HWPFPS H+MEGYAYILTHPGIPT+FYD+FYDW +S+HEQIVKLID RKRQ I+SRS +RILEA+ N+Y+AIIGEK+CMKIGDGSWSPSG+EWTL+TSGH YAVW K
Sbjct:  272 WWPSRAVTFIDNHDTGSTQGHWPFPSHHIMEGYAYILTHPGIPTVFYDHFYDWGNSIHEQIVKLIDIRKRQDINSRSSIRILEAQPNLYSAIIGEKVCMKIGDGSWSPSGREWTLATSGHRYAVWQK 398
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: B9GQ54_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_798380 PE=3 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 7.850e-62
Identity = 101/127 (79.53%), Postives = 116/127 (91.34%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TFIDNHDTGSTQAHWPFPSDH+MEGYAY+LTHPG+PT+FYD+FYDW +S+HEQIVKLID RK Q IHSRS +RI EA+ N+Y+AIIGEK+CMKIGDGSW P+GKEWTL+TSGH YAVW K
Sbjct:  280 WWPSRAVTFIDNHDTGSTQAHWPFPSDHIMEGYAYLLTHPGMPTVFYDHFYDWGNSIHEQIVKLIDIRKHQDIHSRSSIRIFEAQPNLYSAIIGEKVCMKIGDGSWCPAGKEWTLATSGHRYAVWQK 406
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: Q9SGS0_ARATH (T23E18.6 OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 2.983e-61
Identity = 99/127 (77.95%), Postives = 116/127 (91.34%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TF+DNHDTGSTQAHWPFPS HVMEGYAYILTHPGIP +FYD+FYDW  S+H+QIVKLID R+RQ IHSRS VR+L+A+ N+YAAI+GEK+CMK+GDGSW PSG++WTL+TSGH YAVWHK
Sbjct:  286 WWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 412
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: Q8LFG1_ARATH (Alpha-amylase like protein OS=Arabidopsis thaliana GN=At1g76130 PE=2 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 2.983e-61
Identity = 99/127 (77.95%), Postives = 116/127 (91.34%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TF+DNHDTGSTQAHWPFPS HVMEGYAYILTHPGIP +FYD+FYDW  S+H+QIVKLID R+RQ IHSRS VR+L+A+ N+YAAI+GEK+CMK+GDGSW PSG++WTL+TSGH YAVWHK
Sbjct:  287 WWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: D7KTA0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_316534 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 8.679e-61
Identity = 98/127 (77.17%), Postives = 115/127 (90.55%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TF+DNHDTGSTQAHWPFPS H+MEGYAYILTHPGIP +FYD+FYDW  S+H+QIVKLID R+RQ IHSRS +R+L+A+ N+YAAI+GEKLCMK+GDGSW PSG +WTL+TSGH YAVWHK
Sbjct:  287 WWPSRAVTFLDNHDTGSTQAHWPFPSHHIMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTIRVLKAESNLYAAIVGEKLCMKLGDGSWCPSGGDWTLATSGHRYAVWHK 413
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: Q5BLY0_MALDO (Cytosolic alpha-amylase OS=Malus domestica GN=AMY9 PE=3 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.347e-60
Identity = 98/127 (77.17%), Postives = 115/127 (90.55%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TF+DNHDTGSTQAHWPFPS+H+MEGYAYIL HPGIPT+FYD+FYDW  S+H+QIVKLID RKRQ IHSRS + ILEA+ N+Y+AIIGEK+CMKIGDGSW P+ +EWTL+TSGH YAVW+K
Sbjct:  288 WWPSRAVTFLDNHDTGSTQAHWPFPSNHIMEGYAYILMHPGIPTVFYDHFYDWGDSIHDQIVKLIDIRKRQDIHSRSSITILEAQPNLYSAIIGEKICMKIGDGSWCPASREWTLATSGHRYAVWNK 414
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: Q9M6R9_MALDO (Alpha-amylase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.596e-60
Identity = 97/127 (76.38%), Postives = 115/127 (90.55%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSRS TF+DNHDTGSTQAHWPFP++H+MEGY YILTHPGIPT+FYD+FYDW  S+H+QIVKLID RKRQ IHSRS + ILEA+ N+Y+A+IGEK+CMKIGDGSW P+G+EWTL+T GH YAVW+K
Sbjct:  287 WWPSRSVTFLDNHDTGSTQAHWPFPTNHIMEGYTYILTHPGIPTVFYDHFYDWGDSIHDQIVKLIDIRKRQDIHSRSSITILEAQPNLYSAMIGEKVCMKIGDGSWCPAGREWTLATCGHRYAVWNK 413
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: Q5ZFR9_PLAMJ (Alpha-amylase OS=Plantago major GN=aamy1 PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.061e-58
Identity = 97/127 (76.38%), Postives = 113/127 (88.98%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TF+DNHDTGSTQAHWPFPS H+MEGYAYILTHPGIP++FYD+FYDW  ++H+QIVKLID RK QGIHSRS + I+EA+ N+YAA IG+K+ MKIGDGSW PSGKEWTL+TSGH YAVW K
Sbjct:  287 WWPSRAVTFLDNHDTGSTQAHWPFPSHHIMEGYAYILTHPGIPSVFYDHFYDWGTAIHDQIVKLIDIRKHQGIHSRSSIHIIEAQPNLYAANIGDKVSMKIGDGSWCPSGKEWTLATSGHRYAVWQK 413
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Match: C8CII0_SOLTU (Alpha-amylase OS=Solanum tuberosum GN=amyA1 PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 2.613e-57
Identity = 98/126 (77.78%), Postives = 114/126 (90.48%), Query Frame = -3 
Query:   68 WPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 445
            WPSR+ TFIDNHDTGSTQAHWPFPS HVMEGYAYILTHPGIP++FYD+FY+W++S+H+QIVKLI  R+ QGIHSRS +RILEA+ N+YAA I EK+ MKIGDGSWSP+GKEWTL+TSGH YAVW K
Sbjct:  282 WPSRAVTFIDNHDTGSTQAHWPFPSRHVMEGYAYILTHPGIPSVFYDHFYEWDNSMHDQIVKLIAIRRNQGIHSRSSIRILEAQPNLYAATIDEKVSMKIGDGSWSPAGKEWTLATSGHRYAVWQK 407
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TAIR peptide
Match: AT1G76130.1 (| Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 | chr1:28561647-28563914 FORWARD LENGTH=413)

HSP 1 Score: 237.654 bits (605), Expect = 2.089e-63
Identity = 99/127 (77.95%), Postives = 116/127 (91.34%), Query Frame = -3 
Query:   68 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSPSGKEWTLSTSGHNYAVWHK 448
            WWPSR+ TF+DNHDTGSTQAHWPFPS HVMEGYAYILTHPGIP +FYD+FYDW  S+H+QIVKLID R+RQ IHSRS VR+L+A+ N+YAAI+GEK+CMK+GDGSW PSG++WTL+TSGH YAVWHK
Sbjct:  287 WWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413
[Close]
BLAST of Pisum_sativum_v1_Contig3056 vs. TAIR peptide
Match: AT1G69830.1 (| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | chr1:26288518-26293003 REVERSE LENGTH=887)

HSP 1 Score: 144.05 bits (362), Expect = 3.143e-35
Identity = 64/127 (50.39%), Postives = 85/127 (66.93%), Query Frame = -3 
Query:   74 WWPSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGDGSWSP--SGKEWTLSTSGHNYAVW 448
            WWPSR+ TFI+NHDTGSTQ HW FP    M+GYAYILTHPG P +F+D+ +      H +I  L+  R RQ +H RS V I +++ +VYAAII EK+ MKIG G + P    + W+++  G +Y VW
Sbjct:  761 WWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFS---DYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVW 884
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BLAST of Pisum_sativum_v1_Contig3056 vs. TAIR peptide
Match: AT4G25000.1 (| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr4:12852109-12853825 REVERSE LENGTH=423)

HSP 1 Score: 107.457 bits (267), Expect = 3.260e-24
Identity = 60/129 (46.51%), Postives = 80/129 (62.02%), Query Frame = -3 
Query:   68 PSRSATFIDNHDTGSTQAHWPFPSDHVMEGYAYILTHPGIPTIFYDYFYDWNHSVHEQIVKLIDARKRQGIHSRSPVRILEAKHNVYAAIIGEKLCMKIGD----GSWSPSGKEWTLSTSGHNYAVWHK 442
            P  + TFIDNHDT  T   W FPSD V+ GY YILTHPG P IFY+++ +W   + E I KL+  R + GI S S V I  A+ ++Y A+I +K+ MKIG     G+  PS   + L+ SG ++AVW K
Sbjct:  301 PGNAVTFIDNHDTFRT---WVFPSDKVLLGYVYILTHPGTPCIFYNHYIEW--GLKESISKLVAIRNKNGIGSTSSVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPS--NFALAYSGLDFAVWEK 422
The following BLAST results are available for this feature:
BLAST of Pisum_sativum_v1_Contig3056 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
AMY2_HORVU1.191e-3451.15Alpha-amylase type B isozyme OS=Hordeum vulgare GN... [more]
AMY6_HORVU1.555e-3452.67Alpha-amylase type B isozyme OS=Hordeum vulgare GN... [more]
AMY1_HORVU2.653e-3453.44Alpha-amylase type A isozyme OS=Hordeum vulgare GN... [more]
AMY4_HORVU2.246e-3351.15Alpha-amylase type B isozyme (Fragment) OS=Hordeum... [more]
AMY1_ORYSJ3.830e-3351.91Alpha-amylase OS=Oryza sativa subsp. japonica GN=A... [more]
AMY3C_ORYSJ2.483e-3251.91Alpha-amylase isozyme 3C OS=Oryza sativa subsp. ja... [more]
AMY2A_ORYSJ2.483e-3251.15Alpha-amylase isozyme 2A OS=Oryza sativa subsp. ja... [more]
AMY2A_ORYSI2.483e-3251.15Alpha-amylase isozyme 2A OS=Oryza sativa subsp. in... [more]
AMY3_WHEAT4.235e-3251.52Alpha-amylase AMY3 OS=Triticum aestivum GN=AMY1.1 ... [more]
AMY3E_ORYSJ5.531e-3249.62Alpha-amylase isozyme 3E OS=Oryza sativa subsp. ja... [more]
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BLAST of Pisum_sativum_v1_Contig3056 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6TCX9_SOYBN3.765e-6482.68Putative uncharacterized protein OS=Glycine max PE... [more]
B9R8H8_RICCO6.011e-6281.10Alpha-amylase, putative OS=Ricinus communis GN=RCO... [more]
B9GQ54_POPTR7.850e-6279.53Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9SGS0_ARATH2.983e-6177.95T23E18.6 OS=Arabidopsis thaliana PE=3 SV=1[more]
Q8LFG1_ARATH2.983e-6177.95Alpha-amylase like protein OS=Arabidopsis thaliana... [more]
D7KTA0_ARALY8.679e-6177.17Putative uncharacterized protein OS=Arabidopsis ly... [more]
Q5BLY0_MALDO7.347e-6077.17Cytosolic alpha-amylase OS=Malus domestica GN=AMY9... [more]
Q9M6R9_MALDO9.596e-6076.38Alpha-amylase OS=Malus domestica PE=2 SV=1[more]
Q5ZFR9_PLAMJ1.061e-5876.38Alpha-amylase OS=Plantago major GN=aamy1 PE=2 SV=1[more]
C8CII0_SOLTU2.613e-5777.78Alpha-amylase OS=Solanum tuberosum GN=amyA1 PE=2 S... [more]
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BLAST of Pisum_sativum_v1_Contig3056 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT1G76130.12.089e-6377.95| Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 | c... [more]
AT1G69830.13.143e-3550.39| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | c... [more]
AT4G25000.13.260e-2446.51| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 69..127
score: 9.2
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810no descriptioncoord: 69..127
score: 6.9
IPR013780Glycosyl hydrolase, family 13, all-betaGENE3D2.60.40.1180no descriptioncoord: 74..127
score: 3
IPR013781Glycoside hydrolase, subgroup, catalytic coreGENE3D3.20.20.80no descriptioncoord: 2..73
score: 2
IPR017853Glycoside hydrolase, catalytic coreSUPERFAMILY51445(Trans)glycosidasescoord: 3..70
score: 4.4
NoneNo IPR availablePANTHERPTHR10357AMYLASEcoord: 1..104
score: 2
NoneNo IPR availablePANTHERPTHR10357:SF9ALPHA-AMYLASEcoord: 1..104
score: 2
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 73..127
score: 2.6

Sequences
The following sequences are available for this feature:

contig sequence

>Pisum_sativum_v1_Contig3056 ID=Pisum_sativum_v1_Contig3056; Name=Pisum_sativum_v1_Contig3056; organism=Pisum sativum; type=contig; length=450bp
AGCGTGGTCGCGGCGAGGTACACACCCAGTGCCAGTGGAGAGGAAGGCTC
AAGCTACAGGAACACTACTTGTGCCATACAGCATAGTTATGGCCACTGGT
TGATAGTGTCCATTCCTTCCCAGATGGGCTCCATGAGCCATCTCCAATCT
TCATGCAGAGCTTCTCCCCGATGATTGCAGCGTAAACATTGTGCTTGGCT
TCCAGAATCCTAACAGGCGATCTGCTATGAATACCTTGACGCTTGCGAGC
GTCAATCAACTTCACAATCTGCTCATGAACTGAGTGGTTCCAGTCATAAA
AGTAGTCATAGAAAATCGTAGGTATCCCAGGATGAGTCAATATGTAAGCA
TAGCCCTCCATAACATGATCAGAAGGGAAGGGCCAATGAGCCTGAGTTGA
GCCAGTATCATGATTATCTATAAATGTCGCTGATCTTGAAGGCCACCACC
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Annotated Terms
The following terms have been associated with this contig:
Vocabulary: Molecular Function
TermDefinition
GO:0004556alpha-amylase activity
GO:0005509calcium ion binding
GO:0003824catalytic activity
GO:0043169cation binding
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR012850A-amylase_bs_C
IPR013780Glyco_hydro_b
IPR013781Glyco_hydro_catalytic_dom
IPR017853Glycoside_hydrolase_SF