<div> Marker Assisted Selection (MAS) for chickpea<em> Fusarium </em><span style="font-size: 13.1199998855591px; line-height: 17.0559997558594px;"><em>oxysporum</em> wilt resistant genotypes using PCR based molecular </span><span style="font-si
Publication Overview
Abstract The exploration of genetically superior accessions
is the key source of germplasm conservation and
potential breeding material for the future. To meet the
demand of better yielding chickpea cultivars in Pakistan
the present study was organized to select more stable and
resistant lines from indigenous as well as exotic chickpea
germplasm obtained from Plant Genetic Resource Institute
(PGRI), National Agricultural Research Centre, Islamabad,
Pakistan. For the identification and evaluation of chickpea
wilt resistant lines against Fusarium oxysporum f. sp. ciceris
(Schlechtends), the germplasm was tested in the field
for the selection of wilt resistant lines and the PCR based
molecular markers were investigated to use Marker
Assisted Selection (MAS) for selection of the desirable
cultivars. In field trial, 70 % accessions were resistant to
wilt disease, while the remaining 30 % have shown susceptibility
to the disease. A total of 5 RAPD and 15 SSR
markers were screened for molecular based characterization
of wilt response. The data of molecular markers were
scored by the presence (1) and absence (0) of allele and
subjected to statistical analysis. The analysis was based on
coefficient of molecular similarity using UPGMA and
sorted the germplasm into two groups based on disease
response. Among the total used RAPD/SSR primers, only
TA194 SSR marker showed linkage to wilt resistant locus
at 85 % probability. The linkage of a marker was reconfirmed
by receiver operating characteristic curve. The use
of the sorted wilt resistant genotypes through SSR marker
TA194 can make available ample prospect in MAS
breeding for yield improvement of the crop in Pakistan.
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