Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)

Publication Overview
TitleConstruction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)
AuthorsSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S
TypeJournal Article
Journal NameMolecular Breeding
Volume35
Year2015
Page(s)185
CitationSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S. Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.). 2015; 35:185.

Abstract

Field pea (Pisum sativum L.) is a grain legume crop that is cultivated for either human or livestock consumption. Development of varieties adapted to damaging abiotic and biotic stresses is a major objective for field pea breeding. Bacterial blight is a serious disease caused by the pathogenic agents Pseudomonas syringae pv. syringae and Pseudomonas syringae pv. pisi. A recombinant inbred line (RIL) genetic mapping population was generated by crossing the susceptible genotype Kaspa to the resistant genotype PBA Oura. Previously described sets of single-nucleotide polymorphism and simple sequence repeat markers were assigned to a genetic linkage map of the Kaspa × PBA Oura population, which contained 358 markers spanning 1070 cM with an average marker density of 1 locus per 3.0 cM. Combination with multiple previously published maps (including that of Kaspa × Parafield) subsequently generated an integrated structure with much higher marker density of 1 locus per 0.85 cM. The Kaspa × PBA Oura and Kaspa × Parafield RILs were screened at the seedling stage for resistance to both pathovars. Totals of four and two QTLs for resistance to infection by P. syringae pv. syringae were detected in the Kaspa × Parafield and Kaspa × PBA Oura populations, respectively. A single common genomic region associated with P. syringae pv. pisi resistance was identified in both mapping populations. To integrate information on bacterial blight resistance from various QTL mapping studies, the relevant regions were extrapolated on to the integrated map through use of common flanking markers. The resources generated in this study will support map enhancement, identification of marker-trait associations, genomics-assisted breeding, map-based gene isolation and comparative genetics.
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
KaspaKaspacultivar
PBA OuraPBA Ouracultivar
Kaspa_x_PBA OuraKaspa_x_PBA Ourapopulation
Features
This publication contains information about 3,014 features:
Feature NameUniquenameType
Ps001484Ps001484genetic_marker
PsC20526p286PsC20526p286genetic_marker
PsC11597p484PsC11597p484genetic_marker
PsC21112p188PsC21112p188genetic_marker
PsC5240p234PsC5240p234genetic_marker
PsC21549p66PsC21549p66genetic_marker
PsC22864p300PsC22864p300genetic_marker
PsC13109p598PsC13109p598genetic_marker
PsC14530p126PsC14530p126genetic_marker
Ps001631Ps001631genetic_marker
Ps001232Ps001232genetic_marker
PsC12002p94_Ps001310PsC12002p94_Ps001310genetic_marker
PsC22912p327PsC22912p327genetic_marker
Ps001294Ps001294genetic_marker
Ps001318Ps001318genetic_marker
Ps001424Ps001424genetic_marker
Ps001466Ps001466genetic_marker
PsC3880p181PsC3880p181genetic_marker
PsC9520p293PsC9520p293genetic_marker
PsC21248p110PsC21248p110genetic_marker
SNP_100000714SNP_100000714genetic_marker
PsC25617p184PsC25617p184genetic_marker
Ps001397Ps001397genetic_marker
PsC9925p203PsC9925p203genetic_marker
PsC15776p227_Ps001415PsC15776p227_Ps001415genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Bacterial_Blight_Resistance-Sudheesh-2015
Featuremaps
This publication contains information about 3 maps:
Map Name
Pea-Kaspa_x_PBA Oura-RIL
Pea-Integrated_Map-Sudheesh-2015
Pea-Composite_Map-Sudheesh-2015
Properties
Additional details for this publication include:
Property NameValue
URLhttp://link.springer.com/article/10.1007/s11032-015-0376-4