GT624324

EST Overview


Name	GT624324
Unique Name	GT624324
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	461

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT624324 vs. TrEMBL
Match: A2Q320_MEDTR (Epoxide hydrolase OS=Medicago truncatula GN=MtrDRAFT_AC154391g5v2 PE=4 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.013e-37
Identity = 75/94 (79.79%), Postives = 81/94 (86.17%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTGG          WELTA WSG+KIKVPVKFITG+LD+VYT+P +KEY+HGGGFKEDVPNLEEVIVQK VAHFNNQEAAEEISNHIYEFIKKF
Sbjct:  226 FTGGLNYYRNFNLNWELTAPWSGVKIKVPVKFITGDLDMVYTSPHVKEYIHGGGFKEDVPNLEEVIVQKGVAHFNNQEAAEEISNHIYEFIKKF 319

[Close]

BLAST of GT624324 vs. TrEMBL
Match: A2Q324_MEDTR (Epoxide hydrolase OS=Medicago truncatula GN=MtrDRAFT_AC154391g9v2 PE=4 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.112e-35
Identity = 71/94 (75.53%), Postives = 78/94 (82.98%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTG           WEL A W+G+KIKVPVKFITG+LD+VYT+  MKEY+HGGGFKEDVPNLEEVI+QK VAHFNNQEAAEEISNHIYEFIKKF
Sbjct:  226 FTGALNYYRNFNVNWELMAPWNGVKIKVPVKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGVAHFNNQEAAEEISNHIYEFIKKF 319

[Close]

BLAST of GT624324 vs. TrEMBL
Match: A2Q321_MEDTR (Epoxide hydrolase OS=Medicago truncatula GN=MtrDRAFT_AC154391g6v2 PE=4 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.112e-35
Identity = 71/94 (75.53%), Postives = 78/94 (82.98%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTG           WEL A W+G+KIKVPVKFITG+LD+VYT+  MKEY+HGGGFKEDVPNLEEVI+QK VAHFNNQEAAEEISNHIYEFIKKF
Sbjct:  226 FTGALNYYRNFNLNWELMAPWNGVKIKVPVKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGVAHFNNQEAAEEISNHIYEFIKKF 319

[Close]

BLAST of GT624324 vs. TrEMBL
Match: Q8LPE6_CICAR (Epoxide hydrolase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 6.145e-35
Identity = 72/94 (76.60%), Postives = 76/94 (80.85%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTGG          WEL A W G K+ VPVKFITGELD+VYT+  MKEY+HGGGFKEDVPNLEEVIVQK VAHFNNQEAAEEISNHIYEFIKKF
Sbjct:  182 FTGGLNYYRNLNLNWELMAPWRGAKVYVPVKFITGELDMVYTSLNMKEYIHGGGFKEDVPNLEEVIVQKGVAHFNNQEAAEEISNHIYEFIKKF 275

[Close]

BLAST of GT624324 vs. TrEMBL
Match: B7FJM9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 8.025e-35
Identity = 70/94 (74.47%), Postives = 77/94 (81.91%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTG           WEL A W+G+KIKVPVKFITG+LD+VYT+  MKEY+HGGGFKEDVPNLEEVI+QK VAHFNNQEAAEEISNHIYEF KKF
Sbjct:  226 FTGALNYYRNFNVNWELMAPWNGVKIKVPVKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGVAHFNNQEAAEEISNHIYEFTKKF 319

[Close]

BLAST of GT624324 vs. TrEMBL
Match: B7FJ70_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.369e-34
Identity = 70/94 (74.47%), Postives = 77/94 (81.91%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTG           WEL A W+G+KIKVPVKFITG+LD+VYT+  MKEY+HGGGFKEDVPNLEEVI+QK  AHFNNQEAAEEISNHIYEFIKKF
Sbjct:  226 FTGALNYYRNFNLNWELMAPWNGVKIKVPVKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGGAHFNNQEAAEEISNHIYEFIKKF 319

[Close]

BLAST of GT624324 vs. TrEMBL
Match: C6TK81_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 3.728e-32
Identity = 65/94 (69.15%), Postives = 77/94 (81.91%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTGG          WELT  W+ ++IKVPVKFI GELD+VYT+ G+KEY+HGGGFKEDVPNLE+VIVQK VAHFNNQEAAE+ISN+I++FI KF
Sbjct:  225 FTGGLNYYRNINSNWELTTPWTRVQIKVPVKFIAGELDMVYTSLGIKEYIHGGGFKEDVPNLEQVIVQKGVAHFNNQEAAEDISNYIHDFINKF 318

[Close]

BLAST of GT624324 vs. TrEMBL
Match: B7FKA3_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.417e-31
Identity = 62/94 (65.96%), Postives = 77/94 (81.91%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            F+GG          WELTAAW+G +IKVP+KFITG+ D+VYT+ G K+Y+  GGFK+DVPNLEEV++Q+ VAHFNNQEAAE+ISNHIY+FIKKF
Sbjct:  223 FSGGLNFYRNLNLNWELTAAWTGAQIKVPLKFITGDSDLVYTSSGTKQYIESGGFKKDVPNLEEVVIQEGVAHFNNQEAAEDISNHIYDFIKKF 316

[Close]

BLAST of GT624324 vs. TrEMBL
Match: C6TCC6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.156e-31
Identity = 62/94 (65.96%), Postives = 75/94 (79.79%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTGG          WELTA W+G ++KVPVKFITG+LD+VYT+ G K Y+  G FK+DVPNLEEV+VQ+ VAHFNNQEAAE++SNHIY+FIKKF
Sbjct:  223 FTGGLNYYRNFNLNWELTAPWTGAQVKVPVKFITGDLDLVYTSLGTKNYIESGAFKKDVPNLEEVVVQEGVAHFNNQEAAEDVSNHIYDFIKKF 316

[Close]

BLAST of GT624324 vs. TrEMBL
Match: Q39856_SOYBN (Epoxide hydrolase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 5.383e-31
Identity = 64/94 (68.09%), Postives = 75/94 (79.79%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTG           WELTA W+G +IKVPVK+ITGELD+VY +  +KEY+HGGGFK+DVPNLE+VIVQK VAHFNNQEAAEEI N+IY+FI KF
Sbjct:  248 FTGPLNYYRNFNLNWELTAPWTGGQIKVPVKYITGELDMVYNSLNLKEYIHGGGFKQDVPNLEQVIVQKGVAHFNNQEAAEEIDNYIYDFINKF 341

[Close]

BLAST of GT624324 vs. TAIR peptide
Match: AT3G05600.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:1623485-1624704 REVERSE LENGTH=331)

HSP 1 Score: 122.094 bits (305), Expect = 1.341e-28
Identity = 54/97 (55.67%), Postives = 69/97 (71.13%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF*DD 293
            FTGG          WELTA W+G KI+VPVKF+TG+ D+VYTTPGMKEY+HGGGF  DVP L+E++V +D  HF NQE  +E++ HI +F  K  D+
Sbjct:  231 FTGGLNYYRAMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIEDAGHFVNQEKPQEVTAHINDFFTKLRDN 327

[Close]

BLAST of GT624324 vs. TAIR peptide
Match: AT4G02340.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:1035722-1037403 FORWARD LENGTH=324)

HSP 1 Score: 115.931 bits (289), Expect = 9.609e-27
Identity = 52/94 (55.32%), Postives = 66/94 (70.21%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTGG          WELTA W+G++IKVPVKFI G+LDI Y  PG KEY+H GG K+ VP L+EV+V + V HF +QE  +E+++HIY F KKF
Sbjct:  223 FTGGLNYYRALNLSWELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQEKPDEVTDHIYGFFKKF 316

[Close]

BLAST of GT624324 vs. TAIR peptide
Match: AT4G15960.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9045763-9047199 REVERSE LENGTH=375)

HSP 1 Score: 103.605 bits (257), Expect = 4.935e-23
Identity = 47/94 (50.00%), Postives = 61/94 (64.89%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTG           WELTA W+G KI+VPVKFI G+ D+ Y  PG KEY++GGGFK DVP L+E +V K + HF ++E  + I+ HI+ F  KF
Sbjct:  281 FTGPINYYRNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKGLGHFLHEENPDVINQHIHNFFHKF 374

[Close]

BLAST of GT624324 vs. TAIR peptide
Match: AT2G26740.1 (| Symbols: ATSEH, SEH | soluble epoxide hydrolase | chr2:11393148-11394257 REVERSE LENGTH=321)

HSP 1 Score: 98.2117 bits (243), Expect = 2.073e-21
Identity = 47/79 (59.49%), Postives = 56/79 (70.89%), Query Frame = 3

Query:   48 ELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            EL   W G KI+VP KF+ GELD+VY  PG+KEY+HG  FKEDVP LEE +V + VAHF NQE  +EI   I +FI KF
Sbjct:  243 ELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAHFINQEKPQEILQIILDFISKF 321

[Close]

BLAST of GT624324 vs. TAIR peptide
Match: AT2G26750.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr2:11395971-11397085 REVERSE LENGTH=320)

HSP 1 Score: 95.1301 bits (235), Expect = 1.755e-20
Identity = 45/79 (56.96%), Postives = 55/79 (69.62%), Query Frame = 3

Query:   48 ELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            EL   W G KI+VP KF+ GELD+VY  PG+KEY+HG  FKEDVP +EE +V + VAHF NQE  +EI   I +FI  F
Sbjct:  242 ELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHFLNQEKPQEILQIILDFISTF 320

[Close]

BLAST of GT624324 vs. TAIR peptide
Match: AT4G15955.3 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9043872-9045544 REVERSE LENGTH=304)

HSP 1 Score: 90.8929 bits (224), Expect = 3.310e-19
Identity = 43/94 (45.74%), Postives = 57/94 (60.64%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTPGMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKKF 284
            FTG           WEL  + S  K+KVPVKFI G+ D+ Y  PG K+Y+H G FK  VP L+EV+V K V HF ++E  +EIS HI+++   F
Sbjct:  211 FTGPVNYYRNMDRTWELMGSLSNAKVKVPVKFIIGDQDLTYHIPGSKKYIHDGRFKSHVPLLDEVVVIKGVGHFIHEERPDEISKHIHDYFLTF 304

[Close]

BLAST of GT624324 vs. TAIR peptide
Match: AT3G51000.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:18945258-18946499 REVERSE LENGTH=323)

HSP 1 Score: 70.8626 bits (172), Expect = 3.545e-13
Identity = 41/94 (43.62%), Postives = 50/94 (53.19%), Query Frame = 3

Query:    3 FTGGXXXXXXXXXXWELTAAWSGIKIKVPVKFITGELDIVYTTP-GMKEYVHGGGFKEDVPNLEEVIVQKDVAHFNNQEAAEEISNHIYEFIKK 281
            FTG           WE+ A W   KI VP KFI G+ DI Y  P G  EYV G  FK  VPNLE V+++    HF  QE +E++S  I  F+ K
Sbjct:  226 FTGPLNYYRSMDMNWEILAPWQDSKIVVPTKFIAGDKDIGYEGPNGTMEYVKGEVFKIVVPNLEIVVIEGG-HHFIQQEKSEQVSQEILSFLNK 318

The following BLAST results are available for this feature:

BLAST of GT624324 vs. TrEMBL
BLAST of GT624324 vs. TAIR peptide

BLAST of GT624324 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A2Q320_MEDTR	1.013e-37	79.79	Epoxide hydrolase OS=Medicago truncatula GN=MtrDRA...	[more]
A2Q324_MEDTR	2.112e-35	75.53	Epoxide hydrolase OS=Medicago truncatula GN=MtrDRA...	[more]
A2Q321_MEDTR	2.112e-35	75.53	Epoxide hydrolase OS=Medicago truncatula GN=MtrDRA...	[more]
Q8LPE6_CICAR	6.145e-35	76.60	Epoxide hydrolase (Fragment) OS=Cicer arietinum PE...	[more]
B7FJM9_MEDTR	8.025e-35	74.47	Putative uncharacterized protein OS=Medicago trunc...	[more]
B7FJ70_MEDTR	1.369e-34	74.47	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TK81_SOYBN	3.728e-32	69.15	Putative uncharacterized protein OS=Glycine max PE...	[more]
B7FKA3_MEDTR	1.417e-31	65.96	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TCC6_SOYBN	3.156e-31	65.96	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q39856_SOYBN	5.383e-31	68.09	Epoxide hydrolase OS=Glycine max PE=2 SV=1	[more]

BLAST of GT624324 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT3G05600.1	1.341e-28	55.67	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G02340.1	9.609e-27	55.32	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G15960.1	4.935e-23	50.00	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT2G26740.1	2.073e-21	59.49	\| Symbols: ATSEH, SEH \| soluble epoxide hydrolase ...	[more]
AT2G26750.1	1.755e-20	56.96	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G15955.3	3.310e-19	45.74	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT3G51000.1	3.545e-13	43.62	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 28..44 score: 5.7e-10coord: 68..90 score: 5.7
None	No IPR available	GENE3D	3.40.50.1820	no description	coord: 1..93 score: 1.7
None	No IPR available	PANTHER	PTHR10992	ALPHA/BETA HYDROLASE RELATED	coord: 2..92 score: 3.7
None	No IPR available	PANTHER	PTHR10992:SF22	EPOXIDE HYDROLASE	coord: 2..92 score: 3.7
None	No IPR available	SEG	seg	seg	coord: 5..14 score
None	No IPR available	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 1..94 score: 7.4

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT624324 ID=GT624324; Name=GT624324; organism=Lens culinaris; type=EST; length=461bp
GCTTCACTGGAGGATTGAATTACTATAGAAACCTTAATTTAAATTGGGAG
CTCACAGCAGCATGGAGTGGAATCAAAATCAAGGTGCCTGTGAAATTCAT
TACAGGTGAATTAGACATAGTATACACCACGCCCGGTATGAAGGAGTATG
TCCATGGTGGAGGATTCAAGGAAGATGTTCCAAATTTGGAGGAAGTGATC
GTGCAAAAAGATGTTGCTCACTTCAACAATCAAGAAGCTGCAGAGGAGAT
CAGTAATCACATCTACGAATTTATCAAGAAGTTCTGAGATGATATGCGAA
TGAAATAATCATGCCGCTAAGTCTATTTCTTGATTCTTAGTAGTTGCATG
TGGTGTATGCTTAAAATAATTGTTCAGTTGTGCTCTATACCTAAGAAAGT
AATAATGCACTGCATACACGATCCCTTTGTAGTCTAGTTTTTTGAAATGA
ACAGGGTCAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000639	Epox_hydrolase-like

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