GT625158

EST Overview
NameGT625158
Unique NameGT625158
TypeEST
OrganismLens culinaris (lentil)
Sequence length445
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Homology
[Close]
BLAST of GT625158 vs. TrEMBL
Match: D7TB29_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_130.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00000247001 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.364e-13
Identity = 34/55 (61.82%), Postives = 42/55 (76.36%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQNCDSKPI-SGEHYKEE 162
            DDGERM+ACD CGVW+HTRC+ IPD+A VPARF+C RC +    P  + EH K+E
Sbjct:  631 DDGERMLACDGCGVWQHTRCAEIPDSAAVPARFICWRCGSSGQMPTPASEHCKDE 685
[Close]
BLAST of GT625158 vs. TrEMBL
Match: B9INZ8_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_738146 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.155e-12
Identity = 30/38 (78.95%), Postives = 34/38 (89.47%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRC 114
            DDGERM+ACD CGVW+HTRCSGIPD+ PVPA+FVC  C
Sbjct:  642 DDGERMLACDVCGVWQHTRCSGIPDSDPVPAKFVCVGC 679
[Close]
BLAST of GT625158 vs. TrEMBL
Match: B9H427_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_759447 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.155e-12
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQ 117
            DDGERM+ACD CGVW+HTRCSGIPD+  VPA+FVC RC+
Sbjct:  646 DDGERMLACDVCGVWQHTRCSGIPDSDSVPAKFVCLRCR 684
[Close]
BLAST of GT625158 vs. TrEMBL
Match: B9RY52_RICCO (DNA binding protein, putative OS=Ricinus communis GN=RCOM_0814500 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.486e-12
Identity = 28/40 (70.00%), Postives = 36/40 (90.00%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQN 120
            DDGERM+ACD CGVW+HTRCSGI D+  VPA+F+C+RC++
Sbjct:  628 DDGERMLACDVCGVWQHTRCSGILDSDSVPAKFICRRCRD 667
[Close]
BLAST of GT625158 vs. TrEMBL
Match: B9S1T6_RICCO (DNA binding protein, putative OS=Ricinus communis GN=RCOM_0868160 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.178e-11
Identity = 34/77 (44.16%), Postives = 43/77 (55.84%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQNCDSKPISGEHYKEEXXXXXXXXXXXCFGNGAPVLSDV 231
            DDGE+M+ACDTCGVW+HTRC+GI ++  +P+ FVC RC N   +       KEE           C G   P  S V
Sbjct:  625 DDGEKMLACDTCGVWQHTRCAGIDNSDTIPSMFVCLRCMNARRRECK---RKEEVPCQSITTSSTCRGEAVPTGSVV 698
[Close]
BLAST of GT625158 vs. TrEMBL
Match: Q8LJG8_ORYSJ (Os01g0877500 protein OS=Oryza sativa subsp. japonica GN=P0471B04.9 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.412e-10
Identity = 26/38 (68.42%), Postives = 32/38 (84.21%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRC 114
            DDGERM+ACD CGVW+HTRCSGI D   VP +F+C++C
Sbjct:  630 DDGERMLACDVCGVWQHTRCSGISDFDDVPEKFICRKC 667
[Close]
BLAST of GT625158 vs. TrEMBL
Match: A2WXJ6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_04645 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.412e-10
Identity = 26/38 (68.42%), Postives = 32/38 (84.21%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRC 114
            DDGERM+ACD CGVW+HTRCSGI D   VP +F+C++C
Sbjct:  630 DDGERMLACDVCGVWQHTRCSGISDFDDVPEKFICRKC 667
[Close]
BLAST of GT625158 vs. TrEMBL
Match: C5XRD3_SORBI (Putative uncharacterized protein Sb03g041550 OS=Sorghum bicolor GN=Sb03g041550 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.844e-10
Identity = 28/48 (58.33%), Postives = 33/48 (68.75%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQNCDSKPISG 144
            DDGERM+ACD CGVW+HTRCSGI D   VP  F+C++C     K   G
Sbjct:  625 DDGERMLACDICGVWQHTRCSGISDFEEVPENFICRKCATRKGKGRGG 672
[Close]
BLAST of GT625158 vs. TrEMBL
Match: B9T5F9_RICCO (DNA binding protein, putative OS=Ricinus communis GN=RCOM_0052530 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.145e-10
Identity = 32/54 (59.26%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQNCDSKPISGEHYKEE 162
            DDGERM+ACD C VW+HTRC+GI D+  VP  FVC RC  CDS   S +  K E
Sbjct:  621 DDGERMVACDICEVWQHTRCNGIEDSEAVPLLFVCTRC--CDSMIKSRKKVKVE 672
[Close]
BLAST of GT625158 vs. TrEMBL
Match: C5WN10_SORBI (Putative uncharacterized protein Sb01g010120 OS=Sorghum bicolor GN=Sb01g010120 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.195e-9
Identity = 30/50 (60.00%), Postives = 33/50 (66.00%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQNC--DSKPISG 144
            DDGERM+ACD C VW HTRC GI D APVP  F+C  C      + PISG
Sbjct:  648 DDGERMVACDACNVWHHTRCVGIADGAPVPPLFLCISCSGALMAAGPISG 697
[Close]
BLAST of GT625158 vs. SwissProt
Match: Y1342_ARATH (PHD finger protein At1g33420 OS=Arabidopsis thaliana GN=At1g33420 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 4.450e-10
Identity = 26/44 (59.09%), Postives = 32/44 (72.73%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQNCDSK 132
            DDGERM+ACD CGVW HTRC GI +   +P++F+C RC    SK
Sbjct:  613 DDGERMLACDGCGVWHHTRCIGINNADALPSKFLCFRCIELYSK 656
[Close]
BLAST of GT625158 vs. TAIR peptide
Match: AT1G33420.1 (| Symbols: | RING/FYVE/PHD zinc finger superfamily protein | chr1:12121063-12123346 REVERSE LENGTH=697)

HSP 1 Score: 63.5438 bits (153), Expect = 5.305e-11
Identity = 26/44 (59.09%), Postives = 32/44 (72.73%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRCQNCDSK 132
            DDGERM+ACD CGVW HTRC GI +   +P++F+C RC    SK
Sbjct:  613 DDGERMLACDGCGVWHHTRCIGINNADALPSKFLCFRCIELYSK 656
[Close]
BLAST of GT625158 vs. TAIR peptide
Match: AT1G66170.1 (| Symbols: MMD1 | RING/FYVE/PHD zinc finger superfamily protein | chr1:24638793-24641222 REVERSE LENGTH=704)

HSP 1 Score: 60.4622 bits (145), Expect = 4.491e-10
Identity = 23/38 (60.53%), Postives = 28/38 (73.68%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRC 114
            DDGERM++CD C VW+HTRC GI D+  +P  FVC  C
Sbjct:  616 DDGERMISCDVCEVWQHTRCCGIDDSDTLPPLFVCSNC 653
[Close]
BLAST of GT625158 vs. TAIR peptide
Match: AT2G01810.1 (| Symbols: | RING/FYVE/PHD zinc finger superfamily protein | chr2:347537-349952 FORWARD LENGTH=697)

HSP 1 Score: 56.6102 bits (135), Expect = 6.485e-9
Identity = 22/38 (57.89%), Postives = 26/38 (68.42%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRC 114
            DDGERM+ACD C VW HT C+ I D   VP+ F+C  C
Sbjct:  645 DDGERMVACDACKVWHHTLCNSIEDDEAVPSVFLCNMC 682
[Close]
BLAST of GT625158 vs. TAIR peptide
Match: AT5G22260.1 (| Symbols: MS1 | RING/FYVE/PHD zinc finger superfamily protein | chr5:7367707-7370192 REVERSE LENGTH=672)

HSP 1 Score: 55.8398 bits (133), Expect = 1.106e-8
Identity = 21/38 (55.26%), Postives = 26/38 (68.42%), Query Frame = 1 
Query:    1 DDGERMMACDTCGVWRHTRCSGIPDTAPVPARFVCQRC 114
            +DGERM+ CD C VW+HTRC G+     VP  F+CQ C
Sbjct:  624 EDGERMVCCDICEVWQHTRCVGVQHNEEVPRIFLCQSC 661
The following BLAST results are available for this feature:
BLAST of GT625158 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7TB29_VITVI1.364e-1361.82Whole genome shotgun sequence of line PN40024, sca... [more]
B9INZ8_POPTR1.155e-1278.95Predicted protein (Fragment) OS=Populus trichocarp... [more]
B9H427_POPTR1.155e-1276.92Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9RY52_RICCO7.486e-1270.00DNA binding protein, putative OS=Ricinus communis ... [more]
B9S1T6_RICCO2.178e-1144.16DNA binding protein, putative OS=Ricinus communis ... [more]
Q8LJG8_ORYSJ1.412e-1068.42Os01g0877500 protein OS=Oryza sativa subsp. japoni... [more]
A2WXJ6_ORYSI1.412e-1068.42Putative uncharacterized protein OS=Oryza sativa s... [more]
C5XRD3_SORBI1.844e-1058.33Putative uncharacterized protein Sb03g041550 OS=So... [more]
B9T5F9_RICCO3.145e-1059.26DNA binding protein, putative OS=Ricinus communis ... [more]
C5WN10_SORBI1.195e-960.00Putative uncharacterized protein Sb01g010120 OS=So... [more]
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BLAST of GT625158 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
Y1342_ARATH4.450e-1059.09PHD finger protein At1g33420 OS=Arabidopsis thalia... [more]
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BLAST of GT625158 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 4
Match NameE-valueIdentityDescription
AT1G33420.15.305e-1159.09| Symbols: | RING/FYVE/PHD zinc finger superfamil... [more]
AT1G66170.14.491e-1060.53| Symbols: MMD1 | RING/FYVE/PHD zinc finger superf... [more]
AT2G01810.16.485e-957.89| Symbols: | RING/FYVE/PHD zinc finger superfamil... [more]
AT5G22260.11.106e-855.26| Symbols: MS1 | RING/FYVE/PHD zinc finger superfa... [more]
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InterPro
Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001965Zinc finger, PHD-typeSMARTSM00249no descriptioncoord: 1..39
score: 0.
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 1..39
score: 1.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10no descriptioncoord: 1..41
score: 9.4
IPR019787Zinc finger, PHD-fingerPFAMPF00628PHDcoord: 2..41
score: 2.1
NoneNo IPR availableSEGsegsegcoord: 55..65
score

Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT625158 ID=GT625158; Name=GT625158; organism=Lens culinaris; type=EST; length=445bp
GATGATGGAGAGAGAATGATGGCTTGTGACACATGTGGAGTGTGGCGGCA
CACCAGGTGTTCTGGCATTCCTGATACGGCTCCTGTTCCAGCACGTTTTG
TTTGCCAGAGATGCCAAAATTGTGACTCAAAGCCTATATCTGGGGAGCAC
TATAAAGAGGAGACTGTGACTAGTGTTAGTACCACTAGAAGTAGTTGCTT
TGGCAATGGTGCGCCAGTGCTTTCTGATGTTCGTTAGGGCATTGATTAGA
TGTGTATATAAAGCTTTGTTTTTCATTTATTTTGCTTTGGCCTGAAAGAA
CCTGTAGATAAAATATAGGCATTGGTGGGACATATGATGTCTTGTTCATA
TTTCAGGTTCTGCTTTCGTTTGTTTATTTTGTATATACAACAAACAGGTG
GAAGGAAAAAAAAATCTAACTTAAATTGCTTCGAATAGTAAAAAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
Vocabulary: INTERPRO
TermDefinition
IPR001965Znf_PHD
IPR011011Znf_FYVE_PHD
IPR013083Znf_RING/FYVE/PHD
IPR019787Znf_PHD-finger