GT625855

EST Overview
NameGT625855
Unique NameGT625855
TypeEST
OrganismLens culinaris (lentil)
Sequence length484
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Homology
[Close]
BLAST of GT625855 vs. TrEMBL
Match: D7M4N4_ARALY (Ferrochelatase I OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489449 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.190e-17
Identity = 50/72 (69.44%), Postives = 56/72 (77.78%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMD--DPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALGLTPSFITDLADAVIE+LPSA A+  P+   SED +  D   Y VKMFFGSILAF+L LSPK+  AFRN
Sbjct:  394 PALGLTPSFITDLADAVIESLPSAEAMSNPNAVLSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465
[Close]
BLAST of GT625855 vs. TrEMBL
Match: B9RRU8_RICCO (Ferrochelatase OS=Ricinus communis GN=RCOM_0798270 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.190e-17
Identity = 48/71 (67.61%), Postives = 56/71 (78.87%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRNH 214
            PALG T SFITDLADAVIEALPSA AI    ++S E   DP +Y +K+FFGSILAF+L LSPK+I AFRN+
Sbjct:  408 PALGCTSSFITDLADAVIEALPSAKAISTSKSTSEEADYDPFRYAIKLFFGSILAFILLLSPKMIFAFRNN 478
[Close]
BLAST of GT625855 vs. TrEMBL
Match: Q0WW90_ARATH (Ferrochelatase OS=Arabidopsis thaliana GN=At5g26030 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 4.522e-17
Identity = 48/73 (65.75%), Postives = 55/73 (75.34%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPD---NSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALGLTPSFITDLADAVIE+LPSA A+  P+   +S   +  D   Y VKMFFGSILAF+L LSPK+  AFRN
Sbjct:  393 PALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465
[Close]
BLAST of GT625855 vs. TrEMBL
Match: C0Z2M8_ARATH (Ferrochelatase OS=Arabidopsis thaliana GN=AT5G26030 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 4.522e-17
Identity = 48/73 (65.75%), Postives = 55/73 (75.34%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPD---NSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALGLTPSFITDLADAVIE+LPSA A+  P+   +S   +  D   Y VKMFFGSILAF+L LSPK+  AFRN
Sbjct:  393 PALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465
[Close]
BLAST of GT625855 vs. TrEMBL
Match: D7STM2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035351001 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.828e-16
Identity = 46/70 (65.71%), Postives = 54/70 (77.14%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T SFITDLADAVIEALP+A A+     S   +MD PV Y +KMFFGSILAF+L LSPK+I+ F+N
Sbjct:  394 PALGCTSSFITDLADAVIEALPAAKAMTTQSTSKEFNMD-PVNYAIKMFFGSILAFVLLLSPKMISKFKN 462
[Close]
BLAST of GT625855 vs. TrEMBL
Match: A5AY30_VITVI (Ferrochelatase OS=Vitis vinifera GN=VITISV_021593 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.828e-16
Identity = 46/70 (65.71%), Postives = 54/70 (77.14%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T SFITDLADAVIEALP+A A+     S   +MD PV Y +KMFFGSILAF+L LSPK+I+ F+N
Sbjct:  374 PALGCTSSFITDLADAVIEALPAAKAMTTQSTSKEFNMD-PVNYAIKMFFGSILAFVLLLSPKMISKFKN 442
[Close]
BLAST of GT625855 vs. TrEMBL
Match: B9HDQ5_POPTR (Ferrochelatase OS=Populus trichocarpa GN=POPTRDRAFT_1081443 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.900e-15
Identity = 44/70 (62.86%), Postives = 52/70 (74.29%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T SFITDLADAVIEALPSA +     ++S E   D + Y +KM FGS+LAF+L  SPK+ITAFRN
Sbjct:  340 PALGCTSSFITDLADAVIEALPSAKSFSTTRSTSEESDRDFLSYAIKMIFGSVLAFVLLFSPKVITAFRN 409
[Close]
BLAST of GT625855 vs. TrEMBL
Match: C5X8W5_SORBI (Ferrochelatase OS=Sorghum bicolor GN=Sb02g020320 PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.241e-15
Identity = 42/70 (60.00%), Postives = 53/70 (75.71%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T +FI+DLADAV+EALPSA+A+       ++   D + Y  KMFFGSILAFML LSP+L++AFRN
Sbjct:  406 PALGCTSTFISDLADAVVEALPSASALATRKPEDTDSNMDLMHYLTKMFFGSILAFMLLLSPRLVSAFRN 475
[Close]
BLAST of GT625855 vs. TrEMBL
Match: C5XRL6_SORBI (Ferrochelatase OS=Sorghum bicolor GN=Sb04g000740 PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.219e-15
Identity = 41/70 (58.57%), Postives = 53/70 (75.71%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T +FI+DLADAV+EALPSA+A+       ++   D + Y  KMFFGSILAF+L LSP+L++AFRN
Sbjct:  397 PALGCTSTFISDLADAVVEALPSASALATRKPGDTDSSMDLMHYLTKMFFGSILAFVLLLSPRLVSAFRN 466
[Close]
BLAST of GT625855 vs. TrEMBL
Match: B4FK03_MAIZE (Ferrochelatase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.429e-15
Identity = 41/70 (58.57%), Postives = 53/70 (75.71%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T +FI+DLADAV+EALPSA+A+       ++   D + Y  KMFFGSILAF+L LSP+L++AFRN
Sbjct:  404 PALGCTSTFISDLADAVVEALPSASALGTRKPEDTDSSMDLMHYLTKMFFGSILAFILLLSPRLVSAFRN 473
[Close]
BLAST of GT625855 vs. SwissProt
Match: HEMH1_ARATH (Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=HEM15 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 3.258e-18
Identity = 48/73 (65.75%), Postives = 55/73 (75.34%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPD---NSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALGLTPSFITDLADAVIE+LPSA A+  P+   +S   +  D   Y VKMFFGSILAF+L LSPK+  AFRN
Sbjct:  393 PALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465
[Close]
BLAST of GT625855 vs. SwissProt
Match: HEMH1_ORYSJ (Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica GN=Os09g0297000 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.788e-16
Identity = 44/70 (62.86%), Postives = 53/70 (75.71%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T SFI+DLADAV+EALPSA+A+       S+   D + Y  KMFFGSILAF+L LSP+LI+AFRN
Sbjct:  410 PALGCTSSFISDLADAVVEALPSASALVTKKVDESDSDMDLMHYLSKMFFGSILAFVLLLSPRLISAFRN 479
[Close]
BLAST of GT625855 vs. SwissProt
Match: HEMH_HORVU (Ferrochelatase-2, chloroplastic OS=Hordeum vulgare GN=HEMH PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.403e-15
Identity = 40/70 (57.14%), Postives = 51/70 (72.86%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALG T SFI+DLADAV+EALPSA+A+       ++   D + Y  KMF GS+LAF L LSP+L++AFRN
Sbjct:  412 PALGCTSSFISDLADAVVEALPSASAMATRKVKDTDSDMDMMHYLTKMFLGSVLAFFLLLSPRLVSAFRN 481
[Close]
BLAST of GT625855 vs. SwissProt
Match: HEMH_CUCSA (Ferrochelatase-2, chloroplastic OS=Cucumis sativus GN=HEMH PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 9.810e-15
Identity = 40/71 (56.34%), Postives = 49/71 (69.01%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPDNSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRNH 214
            PAL    SFI+DLADAVIEALPSA A+    +S+  D  DP  Y +K+ FGS+LAF+L LSPK    FRN+
Sbjct:  418 PALNCNSSFISDLADAVIEALPSATALAPHTSSTDADDHDPFLYAIKLLFGSVLAFILLLSPKAFMVFRNN 488
[Close]
BLAST of GT625855 vs. TAIR peptide
Match: AT5G26030.2 (| Symbols: FC1, FC-I, ATFC-I | ferrochelatase 1 | chr5:9096675-9098752 FORWARD LENGTH=466)

HSP 1 Score: 90.8929 bits (224), Expect = 3.744e-19
Identity = 48/73 (65.75%), Postives = 55/73 (75.34%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPD---NSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALGLTPSFITDLADAVIE+LPSA A+  P+   +S   +  D   Y VKMFFGSILAF+L LSPK+  AFRN
Sbjct:  393 PALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465
[Close]
BLAST of GT625855 vs. TAIR peptide
Match: AT5G26030.1 (| Symbols: FC1, FC-I, ATFC-I | ferrochelatase 1 | chr5:9096675-9098752 FORWARD LENGTH=466)

HSP 1 Score: 90.8929 bits (224), Expect = 3.744e-19
Identity = 48/73 (65.75%), Postives = 55/73 (75.34%), Query Frame = 2 
Query:    2 PALGLTPSFITDLADAVIEALPSAAAIYAPD---NSSSEDMDDPVKYFVKMFFGSILAFMLFLSPKLITAFRN 211
            PALGLTPSFITDLADAVIE+LPSA A+  P+   +S   +  D   Y VKMFFGSILAF+L LSPK+  AFRN
Sbjct:  393 PALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465
The following BLAST results are available for this feature:
BLAST of GT625855 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7M4N4_ARALY1.190e-1769.44Ferrochelatase I OS=Arabidopsis lyrata subsp. lyra... [more]
B9RRU8_RICCO1.190e-1767.61Ferrochelatase OS=Ricinus communis GN=RCOM_0798270... [more]
Q0WW90_ARATH4.522e-1765.75Ferrochelatase OS=Arabidopsis thaliana GN=At5g2603... [more]
C0Z2M8_ARATH4.522e-1765.75Ferrochelatase OS=Arabidopsis thaliana GN=AT5G2603... [more]
D7STM2_VITVI3.828e-1665.71Whole genome shotgun sequence of line PN40024, sca... [more]
A5AY30_VITVI3.828e-1665.71Ferrochelatase OS=Vitis vinifera GN=VITISV_021593 ... [more]
B9HDQ5_POPTR1.900e-1562.86Ferrochelatase OS=Populus trichocarpa GN=POPTRDRAF... [more]
C5X8W5_SORBI3.241e-1560.00Ferrochelatase OS=Sorghum bicolor GN=Sb02g020320 P... [more]
C5XRL6_SORBI7.219e-1558.57Ferrochelatase OS=Sorghum bicolor GN=Sb04g000740 P... [more]
B4FK03_MAIZE9.429e-1558.57Ferrochelatase OS=Zea mays PE=2 SV=1[more]
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BLAST of GT625855 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 4
Match NameE-valueIdentityDescription
HEMH1_ARATH3.258e-1865.75Ferrochelatase-1, chloroplastic/mitochondrial OS=A... [more]
HEMH1_ORYSJ1.788e-1662.86Ferrochelatase-1, chloroplastic OS=Oryza sativa su... [more]
HEMH_HORVU4.403e-1557.14Ferrochelatase-2, chloroplastic OS=Hordeum vulgare... [more]
HEMH_CUCSA9.810e-1556.34Ferrochelatase-2, chloroplastic OS=Cucumis sativus... [more]
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BLAST of GT625855 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G26030.23.744e-1965.75| Symbols: FC1, FC-I, ATFC-I | ferrochelatase 1 | ... [more]
AT5G26030.13.744e-1965.75| Symbols: FC1, FC-I, ATFC-I | ferrochelatase 1 | ... [more]
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InterPro
Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 39..60
score
NoneNo IPR availableSEGsegsegcoord: 31..40
score

Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT625855 ID=GT625855; Name=GT625855; organism=Lens culinaris; type=EST; length=484bp
CCCTGCCCTTGGTCTTACTCCTTCCTTCATTACTGATCTGGCAGATGCAG
TGATAGAAGCTCTTCCATCGGCAGCAGCAATATATGCCCCAGACAACAGC
AGCTCTGAAGATATGGATGATCCGGTTAAATACTTCGTCAAGATGTTTTT
TGGTTCAATTTTGGCATTCATGTTATTTTTGTCACCCAAATTGATAACAG
CGTTCAGGAATCATAATGTCATTTAGAAGAATACCATCAAGCATAGTTGA
TAAAGCTATTCATAGCAAATTCTTATATTTTTCCCCCCTTGGTTTGACAC
CATTCCACATATAATGTATACATCACAGAGATATGAAAGAAGCAGAAGCG
TTGGTAATAATGAGAACTTGCACGTTCGCTTGGGAACAACCCAGACAGTT
TACTTGTATTTGTATACTAATCTATTATATTTTCGGTTGCTCTTGCAAAA
ATTTGCGCAAGAATATATTTATTTGTTTGAATTA
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