GT618887

EST Overview
NameGT618887
Unique NameGT618887
TypeEST
OrganismLens culinaris (lentil)
Sequence length408
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Lens culinaris unigene v12010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Lens culinaris unigene v12011-01-06
Homology
[Close]
BLAST of GT618887 vs. TrEMBL
Match: B7FHS5_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 5.685e-28
Identity = 56/68 (82.35%), Postives = 64/68 (94.12%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA 206
            F+F+IWKNVPQGAATTCYVALHPS+KGVTGKYF+DCNEF+PSA+A N +L +KLWDFSNKLI SISKA
Sbjct:  257 FSFYIWKNVPQGAATTCYVALHPSVKGVTGKYFVDCNEFKPSAYAKNKLLAKKLWDFSNKLINSISKA 324
[Close]
BLAST of GT618887 vs. TrEMBL
Match: C6TA87_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.389e-26
Identity = 56/68 (82.35%), Postives = 60/68 (88.24%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA 206
            F+F IWKNVPQGAATTCYVALHPSLKGVTGKY  DCNE  PSA ASN +LGRKLWDFSNK+I S+SKA
Sbjct:  256 FSFMIWKNVPQGAATTCYVALHPSLKGVTGKYLQDCNESPPSAHASNELLGRKLWDFSNKMINSLSKA 323
[Close]
BLAST of GT618887 vs. TrEMBL
Match: C6TE58_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.321e-26
Identity = 55/67 (82.09%), Postives = 59/67 (88.06%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISK 203
            FTFF WKN+PQGAATT YVALHPSLKGVTGKYF DCNE QPS  ASN +LGRKLWDFSNK+I S+SK
Sbjct:  133 FTFFAWKNIPQGAATTYYVALHPSLKGVTGKYFRDCNECQPSTHASNELLGRKLWDFSNKMINSLSK 199
[Close]
BLAST of GT618887 vs. TrEMBL
Match: B9HBK4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_819154 PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 6.950e-26
Identity = 54/68 (79.41%), Postives = 60/68 (88.24%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA 206
            F+FF+WKNVPQGAATTCYVALHPSLKGVTGKY++DCN   PSAFA +  L RKLWDFSNKLI S+SKA
Sbjct:  255 FSFFLWKNVPQGAATTCYVALHPSLKGVTGKYYVDCNSLGPSAFARDEALARKLWDFSNKLITSVSKA 322
[Close]
BLAST of GT618887 vs. TrEMBL
Match: A2Q191_MEDTR (Short-chain dehydrogenase/reductase SDR OS=Medicago truncatula GN=MtrDRAFT_AC148343g23v2 PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.712e-24
Identity = 53/68 (77.94%), Postives = 61/68 (89.71%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA 206
            F+F+IWKN   GAATTCYVALHPS+KGVTGKYF+DCNEF+PSA+A N +L +KLWDFSNKLI SISKA
Sbjct:  257 FSFYIWKN---GAATTCYVALHPSVKGVTGKYFVDCNEFKPSAYAKNKLLAKKLWDFSNKLINSISKA 321
[Close]
BLAST of GT618887 vs. TrEMBL
Match: B9SZC4_RICCO (Short-chain dehydrogenase, putative OS=Ricinus communis GN=RCOM_1202730 PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.449e-23
Identity = 50/68 (73.53%), Postives = 58/68 (85.29%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA 206
            F+  +WKNVPQGAATTCY ALHPSLKG +GKYF+DCNE +PSAFA + +L RKLWD+SNKLI S SKA
Sbjct:  255 FSLPLWKNVPQGAATTCYAALHPSLKGASGKYFVDCNEIKPSAFARDDLLARKLWDYSNKLITSASKA 322
[Close]
BLAST of GT618887 vs. TrEMBL
Match: Q9LZ48_ARATH (Putative uncharacterized protein T22P11_130 OS=Arabidopsis thaliana GN=T22P11_130 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.393e-23
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA*FVSIE 224
            F+F++WKN+PQGAATTCYVALHPS+KGVTGKYF DCNE  PS  A +  L +KLWDFS KLI S+SK  ++  +
Sbjct:  274 FSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLINSVSKKNYLGFD 347
[Close]
BLAST of GT618887 vs. TrEMBL
Match: Q8RWJ2_ARATH (Putative uncharacterized protein At5g02540 OS=Arabidopsis thaliana GN=At5g02540 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.393e-23
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA*FVSIE 224
            F+F++WKN+PQGAATTCYVALHPS+KGVTGKYF DCNE  PS  A +  L +KLWDFS KLI S+SK  ++  +
Sbjct:  255 FSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLINSVSKKNYLGFD 328
[Close]
BLAST of GT618887 vs. TrEMBL
Match: Q8LCE7_ARATH (Putativepod-specific dehydrogenase SAC25 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.393e-23
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA*FVSIE 224
            F+F++WKN+PQGAATTCYVALHPS+KGVTGKYF DCNE  PS  A +  L +KLWDFS KLI S+SK  ++  +
Sbjct:  255 FSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLINSVSKKNYLGFD 328
[Close]
BLAST of GT618887 vs. TrEMBL
Match: D7M7U4_ARALY (Short-chain dehydrogenase/reductase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487032 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.227e-22
Identity = 48/67 (71.64%), Postives = 56/67 (83.58%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISK 203
            F+F++WKN+PQGAATTCYVALHPS+KGVTGKYF DCNE  PS  A +  L +KLWDFS KLI S+SK
Sbjct:  255 FSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLINSVSK 321
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT5G02540.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr5:568609-570720 FORWARD LENGTH=331)

HSP 1 Score: 109.768 bits (273), Expect = 5.242e-25
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 3 
Query:    3 FTFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA*FVSIE 224
            F+F++WKN+PQGAATTCYVALHPS+KGVTGKYF DCNE  PS  A +  L +KLWDFS KLI S+SK  ++  +
Sbjct:  255 FSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLINSVSKKNYLGFD 328
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT2G37540.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:15751695-15753820 REVERSE LENGTH=321)

HSP 1 Score: 101.679 bits (252), Expect = 1.428e-22
Identity = 48/66 (72.73%), Postives = 51/66 (77.27%), Query Frame = 3 
Query:    6 TFFIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISK 203
            TF  WKN+PQGAATTCYVALHP L+GVTGKYF DCN   PS FA+N  L  KLWDFS  LI SISK
Sbjct:  256 TFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCNIVAPSKFATNNSLADKLWDFSVFLIDSISK 321
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G23420.3 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12226060-12228562 FORWARD LENGTH=333)

HSP 1 Score: 72.4034 bits (176), Expect = 9.275e-14
Identity = 34/61 (55.74%), Postives = 43/61 (70.49%), Query Frame = 3 
Query:   12 FIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKS 194
            ++ K+VPQGAATTCYVAL+P + GVTG+YF D N  +P     +  L +KLWDFS KL  S
Sbjct:  272 YMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTELAKKLWDFSTKLTDS 332
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G23420.2 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12226288-12228562 FORWARD LENGTH=316)

HSP 1 Score: 72.4034 bits (176), Expect = 9.275e-14
Identity = 34/61 (55.74%), Postives = 43/61 (70.49%), Query Frame = 3 
Query:   12 FIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKS 194
            ++ K+VPQGAATTCYVAL+P + GVTG+YF D N  +P     +  L +KLWDFS KL  S
Sbjct:  255 YMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTELAKKLWDFSTKLTDS 315
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G23420.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12226288-12228562 FORWARD LENGTH=316)

HSP 1 Score: 72.4034 bits (176), Expect = 9.275e-14
Identity = 34/61 (55.74%), Postives = 43/61 (70.49%), Query Frame = 3 
Query:   12 FIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKS 194
            ++ K+VPQGAATTCYVAL+P + GVTG+YF D N  +P     +  L +KLWDFS KL  S
Sbjct:  255 YMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTELAKKLWDFSTKLTDS 315
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G23430.3 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12229171-12231493 FORWARD LENGTH=310)

HSP 1 Score: 71.633 bits (174), Expect = 1.582e-13
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = 3 
Query:   12 FIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSIS 200
            +I K+VPQGAATTCYVAL+P + GV+G+YF D N  +P     +  L +K+WDFS KL  S S
Sbjct:  243 YILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAKKVWDFSTKLTDSQS 305
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G23430.2 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12229171-12231493 FORWARD LENGTH=322)

HSP 1 Score: 71.633 bits (174), Expect = 1.582e-13
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = 3 
Query:   12 FIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSIS 200
            +I K+VPQGAATTCYVAL+P + GV+G+YF D N  +P     +  L +K+WDFS KL  S S
Sbjct:  255 YILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAKKVWDFSTKLTDSQS 317
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G23430.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12229171-12231493 FORWARD LENGTH=320)

HSP 1 Score: 71.633 bits (174), Expect = 1.582e-13
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = 3 
Query:   12 FIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSIS 200
            +I K+VPQGAATTCYVAL+P + GV+G+YF D N  +P     +  L +K+WDFS KL  S S
Sbjct:  253 YILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAKKVWDFSTKLTDSQS 315
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G11410.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:6945127-6947186 REVERSE LENGTH=317)

HSP 1 Score: 68.5514 bits (166), Expect = 1.339e-12
Identity = 29/58 (50.00%), Postives = 42/58 (72.41%), Query Frame = 3 
Query:   12 FIWKNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKL 185
            ++ K++PQGAATTCY ALHP  KGV+G+Y +D N   P++   +  L +KLW+FS +L
Sbjct:  254 YVLKSIPQGAATTCYAALHPQAKGVSGEYLMDNNISDPNSQGKDKDLAKKLWEFSLRL 311
[Close]
BLAST of GT618887 vs. TAIR peptide
Match: AT4G24050.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12497287-12499661 FORWARD LENGTH=332)

HSP 1 Score: 68.1662 bits (165), Expect = 1.749e-12
Identity = 34/64 (53.12%), Postives = 38/64 (59.38%), Query Frame = 3 
Query:   21 KNVPQGAATTCYVALHPSLKGVTGKYFLDCNEFQPSAFASNGILGRKLWDFSNKLIKSISKA*F 212
            K VPQ AATTCYVA +P L  V+GKYF DCNE  PS   +N     KLW  S  L+   SK  F
Sbjct:  265 KTVPQAAATTCYVATNPRLVNVSGKYFTDCNETTPSGLGTNSSEATKLWAASEILVTQHSKTSF 328
The following BLAST results are available for this feature:
BLAST of GT618887 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B7FHS5_MEDTR5.685e-2882.35Putative uncharacterized protein OS=Medicago trunc... [more]
C6TA87_SOYBN2.389e-2682.35Putative uncharacterized protein OS=Glycine max PE... [more]
C6TE58_SOYBN5.321e-2682.09Putative uncharacterized protein (Fragment) OS=Gly... [more]
B9HBK4_POPTR6.950e-2679.41Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
A2Q191_MEDTR1.712e-2477.94Short-chain dehydrogenase/reductase SDR OS=Medicag... [more]
B9SZC4_RICCO1.449e-2373.53Short-chain dehydrogenase, putative OS=Ricinus com... [more]
Q9LZ48_ARATH9.393e-2364.86Putative uncharacterized protein T22P11_130 OS=Ara... [more]
Q8RWJ2_ARATH9.393e-2364.86Putative uncharacterized protein At5g02540 OS=Arab... [more]
Q8LCE7_ARATH9.393e-2364.86Putativepod-specific dehydrogenase SAC25 OS=Arabid... [more]
D7M7U4_ARALY1.227e-2271.64Short-chain dehydrogenase/reductase family protein... [more]
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BLAST of GT618887 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT5G02540.15.242e-2564.86| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT2G37540.11.428e-2272.73| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G23420.39.275e-1455.74| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G23420.29.275e-1455.74| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G23420.19.275e-1455.74| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G23430.31.582e-1353.97| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G23430.21.582e-1353.97| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G23430.11.582e-1353.97| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G11410.11.339e-1250.00| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
AT4G24050.11.749e-1253.13| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
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InterPro
Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002198Short-chain dehydrogenase/reductase SDRPANTHERPTHR19410SHORT-CHAIN DEHYDROGENASES/REDUCTASE FAMILY MEMBERcoord: 1..37
score: 2.7
NoneNo IPR availablePANTHERPTHR19410:SF98RETINOL DEHYDROGENASEcoord: 1..37
score: 2.7

Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT618887 ID=GT618887; Name=GT618887; organism=Lens culinaris; type=EST; length=408bp
TGTTCACGTTTTTCATATGGAAGAATGTCCCACAGGGAGCTGCCACAACT
TGCTATGTTGCACTGCATCCAAGCTTGAAAGGGGTAACTGGGAAGTACTT
TTTGGATTGTAATGAGTTTCAACCAAGTGCATTTGCTTCAAATGGAATCT
TAGGAAGAAAGCTTTGGGATTTTAGCAACAAGTTGATTAAATCAATTTCA
AAAGCTTGATTTGTCTCTATAGAGACAGAGTCATTCATCACATAATTGTT
GTATCAATCAAAGCCTATTATTGTTAATTAAGATACCCGCATATAATTTA
TAAGTTCTATGAAGAAAAATATATATAGATATCTATGATTTGTATTACTA
TTATGTGCTCGTAATATAGAAGTGTTGTTTTGTGTATGAGACATCTTCTT
CCATGCTT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR002198DH_sc/Rdtase_SDR