GT619625

EST Overview
NameGT619625
Unique NameGT619625
TypeEST
OrganismLens culinaris (lentil)
Sequence length338
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Lens culinaris unigene v12010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide2011-02-03
Homology
[Close]
BLAST of GT619625 vs. TrEMBL
Match: Q9SXU0_CICAR (Aspartic proteinase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.451e-15
Identity = 38/38 (100.00%), Postives = 38/38 (100.00%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA
Sbjct:  167 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 204
[Close]
BLAST of GT619625 vs. TrEMBL
Match: Q2HRQ7_MEDTR (Peptidase A1, pepsin OS=Medicago truncatula GN=MtrDRAFT_AC157894g38v2 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.895e-15
Identity = 37/38 (97.37%), Postives = 38/38 (100.00%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            FD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA
Sbjct:  359 FDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 396
[Close]
BLAST of GT619625 vs. TrEMBL
Match: Q948P0_SOYBN (Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.513e-15
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 112
            FD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:  471 FDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 507
[Close]
BLAST of GT619625 vs. TrEMBL
Match: C6TIU2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.513e-15
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 112
            FD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:  107 FDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 143
[Close]
BLAST of GT619625 vs. TrEMBL
Match: C6TDL8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.513e-15
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 112
            FD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:  140 FDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 176
[Close]
BLAST of GT619625 vs. TrEMBL
Match: Q8H948_SOYBN (Aspartic proteinase 5 (Fragment) OS=Glycine max GN=soyAP5 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 7.961e-14
Identity = 34/38 (89.47%), Postives = 37/38 (97.37%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            FDIPPP+GPLWILGDVFMR YHTVFDYGNL+VGFA+AA
Sbjct:  138 FDIPPPRGPLWILGDVFMRVYHTVFDYGNLRVGFAKAA 175
[Close]
BLAST of GT619625 vs. TrEMBL
Match: Q93XR0_IPOBA (Putative aspartic protease OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.774e-13
Identity = 34/37 (91.89%), Postives = 36/37 (97.30%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            D+PPP+GPLWILGDVFM AYHTVFDYGNLQVGFAEAA
Sbjct:  468 DVPPPQGPLWILGDVFMGAYHTVFDYGNLQVGFAEAA 504
[Close]
BLAST of GT619625 vs. TrEMBL
Match: Q0JKM8_ORYSJ (Os01g0663400 protein OS=Oryza sativa subsp. japonica GN=Os01g0663400 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.025e-13
Identity = 34/37 (91.89%), Postives = 36/37 (97.30%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            DIPPP+GPLWILGDVFM AYHTVFDYGNL+VGFAEAA
Sbjct:  486 DIPPPRGPLWILGDVFMGAYHTVFDYGNLKVGFAEAA 522
[Close]
BLAST of GT619625 vs. TrEMBL
Match: B8A762_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_03158 PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.025e-13
Identity = 34/37 (91.89%), Postives = 36/37 (97.30%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            DIPPP+GPLWILGDVFM AYHTVFDYGNL+VGFAEAA
Sbjct:  486 DIPPPRGPLWILGDVFMGAYHTVFDYGNLKVGFAEAA 522
[Close]
BLAST of GT619625 vs. TrEMBL
Match: B9I6C6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834438 PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.951e-13
Identity = 32/37 (86.49%), Postives = 36/37 (97.30%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            D+PPP+GPLWILGDVFM AYHT+FDYGNL+VGFAEAA
Sbjct:  471 DVPPPRGPLWILGDVFMGAYHTIFDYGNLEVGFAEAA 507
[Close]
BLAST of GT619625 vs. SwissProt
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 8.068e-13
Identity = 30/37 (81.08%), Postives = 35/37 (94.59%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            DIPPP+GPLWILGDVFM AYHTVFDYG ++VGFA++A
Sbjct:  473 DIPPPRGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509
[Close]
BLAST of GT619625 vs. SwissProt
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.054e-12
Identity = 30/37 (81.08%), Postives = 34/37 (91.89%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            DIPPP+GPLWILGDVFM  YHTVFDYG L++GFA+AA
Sbjct:  472 DIPPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508
[Close]
BLAST of GT619625 vs. SwissProt
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 1.376e-12
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            D+ PP GPLWILGDVFM  YHTVFDYGNL+VGFAEAA
Sbjct:  437 DVAPPHGPLWILGDVFMGQYHTVFDYGNLRVGFAEAA 473
[Close]
BLAST of GT619625 vs. SwissProt
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.376e-12
Identity = 31/38 (81.58%), Postives = 34/38 (89.47%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            FDIPPP+GPLWILGDVFM  YHTVFD+G L+VG AEAA
Sbjct:  476 FDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513
[Close]
BLAST of GT619625 vs. SwissProt
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 3.066e-12
Identity = 29/38 (76.32%), Postives = 35/38 (92.11%), Query Frame = 2 
Query:    2 FDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            FDIPPP+GPLWILGDVFM AYHTVFD+G  ++GFA++A
Sbjct:  459 FDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496
[Close]
BLAST of GT619625 vs. SwissProt
Match: CARDH_CYNCA (Cardosin-H (Fragments) OS=Cynara cardunculus PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.288e-10
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:   23 GPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            GPLWILGDVFMR YHTVFDYGNL VGFAEAA
Sbjct:  235 GPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 265
[Close]
BLAST of GT619625 vs. SwissProt
Match: CARDG_CYNCA (Cardosin-G (Fragments) OS=Cynara cardunculus PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.288e-10
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:   23 GPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            GPLWILGDVFMR YHTVFDYGNL VGFAEAA
Sbjct:  236 GPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 266
[Close]
BLAST of GT619625 vs. SwissProt
Match: CARDF_CYNCA (Cardosin-F (Fragments) OS=Cynara cardunculus PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.288e-10
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:   23 GPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            GPLWILGDVFMR YHTVFDYGNL VGFAEAA
Sbjct:  251 GPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 281
[Close]
BLAST of GT619625 vs. SwissProt
Match: CARDE_CYNCA (Cardosin-E (Fragments) OS=Cynara cardunculus PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.288e-10
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:   23 GPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            GPLWILGDVFMR YHTVFDYGNL VGFAEAA
Sbjct:  194 GPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 224
[Close]
BLAST of GT619625 vs. SwissProt
Match: CARDA_CYNCA (Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.288e-10
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:   23 GPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            GPLWILGDVFMR YHTVFDYGNL VGFAEAA
Sbjct:  474 GPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 504
[Close]
BLAST of GT619625 vs. TAIR peptide
Match: AT1G62290.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 72.0182 bits (175), Expect = 8.367e-14
Identity = 31/36 (86.11%), Postives = 33/36 (91.67%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 112
            DIPPP+GPLWILGDVFM  YHTVFD+GN QVGFAEA
Sbjct:  477 DIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512
[Close]
BLAST of GT619625 vs. TAIR peptide
Match: AT1G62290.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 72.0182 bits (175), Expect = 8.367e-14
Identity = 31/36 (86.11%), Postives = 33/36 (91.67%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 112
            DIPPP+GPLWILGDVFM  YHTVFD+GN QVGFAEA
Sbjct:  477 DIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512
[Close]
BLAST of GT619625 vs. TAIR peptide
Match: AT1G11910.1 (| Symbols: APA1, ATAPA1 | aspartic proteinase A1 | chr1:4017119-4019874 REVERSE LENGTH=506)

HSP 1 Score: 70.0922 bits (170), Expect = 3.179e-13
Identity = 30/37 (81.08%), Postives = 33/37 (89.19%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            D+ PP+GPLWILGDVFM  YHTVFD+GN QVGFAEAA
Sbjct:  470 DVAPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEAA 506
[Close]
BLAST of GT619625 vs. TAIR peptide
Match: AT4G04460.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=504)

HSP 1 Score: 65.855 bits (159), Expect = 5.996e-12
Identity = 28/37 (75.68%), Postives = 32/37 (86.49%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            DI PP+GPLWILGD+FM  YHTVFDYG  +VGFA+AA
Sbjct:  468 DIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504
[Close]
BLAST of GT619625 vs. TAIR peptide
Match: AT4G04460.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=508)

HSP 1 Score: 65.855 bits (159), Expect = 5.996e-12
Identity = 28/37 (75.68%), Postives = 32/37 (86.49%), Query Frame = 2 
Query:    5 DIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 115
            DI PP+GPLWILGD+FM  YHTVFDYG  +VGFA+AA
Sbjct:  472 DIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508
The following BLAST results are available for this feature:
BLAST of GT619625 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9SXU0_CICAR1.451e-15100.00Aspartic proteinase (Fragment) OS=Cicer arietinum ... [more]
Q2HRQ7_MEDTR1.895e-1597.37Peptidase A1, pepsin OS=Medicago truncatula GN=Mtr... [more]
Q948P0_SOYBN5.513e-1597.30Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=... [more]
C6TIU2_SOYBN5.513e-1597.30Putative uncharacterized protein OS=Glycine max PE... [more]
C6TDL8_SOYBN5.513e-1597.30Putative uncharacterized protein OS=Glycine max PE... [more]
Q8H948_SOYBN7.961e-1489.47Aspartic proteinase 5 (Fragment) OS=Glycine max GN... [more]
Q93XR0_IPOBA1.774e-1391.89Putative aspartic protease OS=Ipomoea batatas PE=2... [more]
Q0JKM8_ORYSJ3.025e-1391.89Os01g0663400 protein OS=Oryza sativa subsp. japoni... [more]
B8A762_ORYSI3.025e-1391.89Putative uncharacterized protein OS=Oryza sativa s... [more]
B9I6C6_POPTR3.951e-1386.49Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
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BLAST of GT619625 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
ASPR1_ORYSJ8.068e-1381.08Aspartic proteinase oryzasin-1 OS=Oryza sativa sub... [more]
ASPR_HORVU1.054e-1281.08Phytepsin OS=Hordeum vulgare PE=1 SV=1[more]
CYPR1_CYNCA1.376e-1283.78Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO... [more]
ASPR_CUCPE1.376e-1281.58Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1[more]
ASPRX_ORYSJ3.066e-1276.32Aspartic proteinase OS=Oryza sativa subsp. japonic... [more]
CARDH_CYNCA1.288e-1093.55Cardosin-H (Fragments) OS=Cynara cardunculus PE=1 ... [more]
CARDG_CYNCA1.288e-1093.55Cardosin-G (Fragments) OS=Cynara cardunculus PE=1 ... [more]
CARDF_CYNCA1.288e-1093.55Cardosin-F (Fragments) OS=Cynara cardunculus PE=1 ... [more]
CARDE_CYNCA1.288e-1093.55Cardosin-E (Fragments) OS=Cynara cardunculus PE=1 ... [more]
CARDA_CYNCA1.288e-1093.55Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 ... [more]
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BLAST of GT619625 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 5
Match NameE-valueIdentityDescription
AT1G62290.28.367e-1486.11| Symbols: | Saposin-like aspartyl protease famil... [more]
AT1G62290.18.367e-1486.11| Symbols: | Saposin-like aspartyl protease famil... [more]
AT1G11910.13.179e-1381.08| Symbols: APA1, ATAPA1 | aspartic proteinase A1 |... [more]
AT4G04460.25.996e-1275.68| Symbols: | Saposin-like aspartyl protease famil... [more]
AT4G04460.15.996e-1275.68| Symbols: | Saposin-like aspartyl protease famil... [more]
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Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT619625 ID=GT619625; Name=GT619625; organism=Lens culinaris; type=EST; length=338bp
GTTCGACATTCCTCCTCCAAAGGGTCCACTTTGGATTCTTGGTGATGTTT
TCATGAGGGCATATCACACTGTTTTCGACTATGGAAATCTCCAAGTTGGT
TTTGCTGAAGCTGCCTAGTTATGAAGTTGTGGATGAATGGATGATTATGT
CAAGGAATATTTTCATAATTTCGTTTCTGCATTGTAAATAAGTTGTACTA
CTTTGTAACTATAGCTCAGGTTCACTTCACGGATTCATATATGTATGTAT
TTATCTATGTCTATGCACGTACGTTAAGATAAGACGTGAAAATATACACT
TAAAAGTTTATGTGATCTGACATGAACGATTTTCATTT
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