GT620683

EST Overview


Name	GT620683
Unique Name	GT620683
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	706

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT620683 vs. TrEMBL
Match: Q8H8C9_ORYSJ (AMP-dependent synthetase and ligase family protein, putative, expressed OS=Oryza sativa subsp. japonica GN=OJ1006F06.17 PE=4 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 6.979e-40
Identity = 81/117 (69.23%), Postives = 97/117 (82.91%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY +++     + I I   ENIS+IE+E VI+ HPAV+EAAVV RPD+YWGETPCAFVKLK+G NATE EII FCRERLPH+MAP+TVVF DLPKTSTGKTQK++L+EKA+AMG
Sbjct:  424 HPDGYIQLKDR--AKDIIISGGENISSIEVESVIFSHPAVLEAAVVARPDDYWGETPCAFVKLKDGANATEGEIISFCRERLPHYMAPKTVVFDDLPKTSTGKTQKFVLREKARAMG 538

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BLAST of GT620683 vs. TrEMBL
Match: Q0DVE7_ORYSJ (Os03g0133600 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os03g0133600 PE=4 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 6.979e-40
Identity = 81/117 (69.23%), Postives = 97/117 (82.91%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY +++     + I I   ENIS+IE+E VI+ HPAV+EAAVV RPD+YWGETPCAFVKLK+G NATE EII FCRERLPH+MAP+TVVF DLPKTSTGKTQK++L+EKA+AMG
Sbjct:  375 HPDGYIQLKDR--AKDIIISGGENISSIEVESVIFSHPAVLEAAVVARPDDYWGETPCAFVKLKDGANATEGEIISFCRERLPHYMAPKTVVFDDLPKTSTGKTQKFVLREKARAMG 489

[Close]

BLAST of GT620683 vs. TrEMBL
Match: A2XC55_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_09875 PE=4 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 6.979e-40
Identity = 81/117 (69.23%), Postives = 97/117 (82.91%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY +++     + I I   ENIS+IE+E VI+ HPAV+EAAVV RPD+YWGETPCAFVKLK+G NATE EII FCRERLPH+MAP+TVVF DLPKTSTGKTQK++L+EKA+AMG
Sbjct:  424 HPDGYIQLKDR--AKDIIISGGENISSIEVESVIFSHPAVLEAAVVARPDDYWGETPCAFVKLKDGANATEGEIISFCRERLPHYMAPKTVVFDDLPKTSTGKTQKFVLREKARAMG 538

[Close]

BLAST of GT620683 vs. TrEMBL
Match: B9H5H2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_761596 PE=4 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 4.524e-39
Identity = 79/117 (67.52%), Postives = 93/117 (79.49%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY  +E     + I I   ENIS+IE+E  ++ HPAV+EAA+VGRPD+YWGETPCAFVKLKEG NA  EE+IKFCR+ LPH+M PRTVVF +LPKTSTGK QKY+LKEKAKAMG
Sbjct:  427 HPDGY--IELKDRSKDIIISGGENISSIEVESALFSHPAVLEAAIVGRPDDYWGETPCAFVKLKEGCNANAEELIKFCRDHLPHYMTPRTVVFQELPKTSTGKVQKYVLKEKAKAMG 541

[Close]

BLAST of GT620683 vs. TrEMBL
Match: C5WZ16_SORBI (Putative uncharacterized protein Sb01g048050 OS=Sorghum bicolor GN=Sb01g048050 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 2.245e-38
Identity = 78/117 (66.67%), Postives = 96/117 (82.05%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY  +E     + I I   ENIS+IE+E VI+ HPAV+EAAVV RPD++WGETPCAFVKLK+G +ATE EII FCRERLPH+MAP+TVVF D+PKTSTGKTQK++L++KA+AMG
Sbjct:  422 HPDGY--IELKDRAKDIIISGGENISSIEVESVIFSHPAVLEAAVVARPDDHWGETPCAFVKLKDGASATEAEIISFCRERLPHYMAPKTVVFEDMPKTSTGKTQKFVLRDKARAMG 536

[Close]

BLAST of GT620683 vs. TrEMBL
Match: B9S6S7_RICCO (AMP dependent ligase, putative OS=Ricinus communis GN=RCOM_0873210 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 2.245e-38
Identity = 76/117 (64.96%), Postives = 95/117 (81.20%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY  V+    L+ I I   ENIST+E+E V+Y HPA+ EAAVV RPD++WG+TPCAFVKLKEG+  +E++IIKFCR+RLPH+MAPRTV+F DLP+TSTGK QK+IL+EKAK MG
Sbjct:  364 HPDGYIEVKDR--LKDIIISGGENISTVEVETVLYSHPAIFEAAVVARPDDHWGQTPCAFVKLKEGFVVSEQDIIKFCRDRLPHYMAPRTVIFEDLPRTSTGKVQKFILREKAKTMG 478

[Close]

BLAST of GT620683 vs. TrEMBL
Match: D7U6N8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_210.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00003832001 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 3.242e-37
Identity = 73/117 (62.39%), Postives = 94/117 (80.34%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY  +E    L+ I I   ENIST+E+E V+Y HPA++EAAVV RPD +WG+TPCAFVKLKEG++   +EI+KFCR+ LPH+MAP+TV+F DLP+TSTGK QK+IL+EKAKA+G
Sbjct:   36 HPDGY--IEMKDRLKDIIISGGENISTVEVETVLYNHPAILEAAVVARPDNHWGQTPCAFVKLKEGFDVDAQEILKFCRDHLPHYMAPKTVIFEDLPRTSTGKIQKFILREKAKALG 150

[Close]

BLAST of GT620683 vs. TrEMBL
Match: C5WZU1_SORBI (Putative uncharacterized protein Sb01g048090 OS=Sorghum bicolor GN=Sb01g048090 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 3.242e-37
Identity = 77/117 (65.81%), Postives = 94/117 (80.34%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY +++     + I I   ENIS+IE+E V++GHPAV++AAVV RPD++WGETPCAFV LK G  AT ++II FCR RLPH+MAPRTVVFSDLPKTSTGKTQKY+L+EKA AMG
Sbjct:  430 HRDGYIQIKDR--SKDIIISGGENISSIEVESVLFGHPAVLDAAVVARPDDHWGETPCAFVTLKNGATATADDIIGFCRARLPHYMAPRTVVFSDLPKTSTGKTQKYLLREKATAMG 544

[Close]

BLAST of GT620683 vs. TrEMBL
Match: C4J0R9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 3.242e-37
Identity = 75/117 (64.10%), Postives = 96/117 (82.05%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY +++     + I I   ENIS+IE+E V++GHPAV++AAVV RPD++WGE PCAFV L++G  AT ++II+FCR RLPH+MAPRTVVFSDLPKTSTGKTQKY+L+EKA+AMG
Sbjct:  359 HPNGYIQLKDR--SKDIIISGGENISSIEVESVLFGHPAVLDAAVVARPDDHWGEAPCAFVTLRDGARATADDIIRFCRARLPHYMAPRTVVFSDLPKTSTGKTQKYLLREKARAMG 473

[Close]

BLAST of GT620683 vs. TrEMBL
Match: C0PGQ6_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 3.242e-37
Identity = 75/117 (64.10%), Postives = 96/117 (82.05%), Query Frame = -2

Query:  160 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 510
            H  GY +++     + I I   ENIS+IE+E V++GHPAV++AAVV RPD++WGE PCAFV L++G  AT ++II+FCR RLPH+MAPRTVVFSDLPKTSTGKTQKY+L+EKA+AMG
Sbjct:  435 HPNGYIQLKDR--SKDIIISGGENISSIEVESVLFGHPAVLDAAVVARPDDHWGEAPCAFVTLRDGARATADDIIRFCRARLPHYMAPRTVVFSDLPKTSTGKTQKYLLREKARAMG 549

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BLAST of GT620683 vs. SwissProt
Match: ACSF2_DANRE (Acyl-CoA synthetase family member 2, mitochondrial OS=Danio rerio GN=acsf2 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.982e-16
Identity = 44/113 (38.94%), Postives = 68/113 (60.18%), Query Frame = -2

Query:  169 YGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEKAK 504
            + Y ++E    ++ + I   ENI   E+E  ++ HP ++EA VVG  DE  GE  CA ++LKEG   T EEI  +C+ ++ H+  PR ++F  D P T TGK QK+ L+E+ +
Sbjct:  491 FAYCKIEGR--IKDLIIRGGENIYPAEIEQFLHTHPKILEAQVVGVKDERMGEEVCACIRLKEGQECTVEEIKAYCKGKIAHYKVPRYILFVQDYPLTITGKIQKHKLRERTE 601

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BLAST of GT620683 vs. SwissProt
Match: YNGI_BACSU (Putative acyl-CoA synthetase yngI OS=Bacillus subtilis GN=yngI PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.371e-15
Identity = 45/114 (39.47%), Postives = 69/114 (60.53%), Query Frame = -2

Query:  163 GYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFS-DLPKTSTGKTQKYILKEKAKAM 501
            GY R+     L+ + I   ENI   E+E  +Y HPAV++  VVG PD  +GE   A++KLK+G + + +E+  +C+ ++     PR V+F+ D P T++GK QKY L+EK   M
Sbjct:  431 GYCRITGR--LKDMLIRGGENIYPREIEEFLYQHPAVLDVQVVGVPDAKFGEEAAAWIKLKDGKSVSPDELKAYCKGKIARHKIPRYVIFTDDYPMTASGKIQKYKLREKTIEM 542

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BLAST of GT620683 vs. SwissProt
Match: ACSF2_RAT (Acyl-CoA synthetase family member 2, mitochondrial OS=Rattus norvegicus GN=Acsf2 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.749e-15
Identity = 43/91 (47.25%), Postives = 56/91 (61.54%), Query Frame = -2

Query:  175 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSD-LPKTSTGKTQKYILKEK 444
            ENI   ELE   + HP V EA VVG  D+  GE  CA ++LK G   TEEEI  FC+ ++ HF  PR +VF +  P T +GK QK+ L+E+
Sbjct:  518 ENIYPAELEDFFHKHPQVQEAQVVGVKDDRMGEEICACIRLKSGETTTEEEIKAFCKGKISHFKIPRYIVFVEGYPLTVSGKIQKFKLREQ 608

[Close]

BLAST of GT620683 vs. SwissProt
Match: MENE_GEOSW (2-succinylbenzoate--CoA ligase OS=Geobacillus sp. (strain WCH70) GN=menE PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 7.509e-15
Identity = 38/90 (42.22%), Postives = 56/90 (62.22%), Query Frame = -2

Query:  178 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSD-LPKTSTGKTQKYILKE 444
            EN+   E+E V+  H AV EA V G  DE WG+ PCAFVK K GY+ T E++ +FC+  L  +  P+ + F D LP+ ++ K  ++ LK+
Sbjct:  393 ENVYPAEIEAVLLSHEAVEEAGVTGIDDETWGQVPCAFVKRKRGYSVTVEQLKQFCQAHLAKYKIPKQIYFVDELPRNASQKLLRHQLKQ 482

[Close]

BLAST of GT620683 vs. SwissProt
Match: ACSF2_BOVIN (Acyl-CoA synthetase family member 2, mitochondrial OS=Bos taurus GN=ACSF2 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.281e-14
Identity = 42/91 (46.15%), Postives = 56/91 (61.54%), Query Frame = -2

Query:  175 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEK 444
            ENI   ELE   + HP V E  VVG  D+  GE  CA ++LKEG   T EEI  FC+ ++ HF  PR +VF ++ P T +GK QK+ L+E+
Sbjct:  518 ENIYPAELEDFFHTHPQVQEVQVVGVKDDRMGEEICACIRLKEGEKTTAEEIKAFCKGKISHFKIPRYIVFVTNYPLTVSGKIQKFKLREQ 608

[Close]

BLAST of GT620683 vs. SwissProt
Match: ACSF2_MOUSE (Acyl-CoA synthetase family member 2, mitochondrial OS=Mus musculus GN=Acsf2 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.853e-14
Identity = 43/91 (47.25%), Postives = 54/91 (59.34%), Query Frame = -2

Query:  175 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSD-LPKTSTGKTQKYILKEK 444
            ENI   ELE     HP V EA VVG  DE  GE  CA ++LK G   T EEI  FC+ ++ HF  PR +VF +  P T +GK QK+ L+E+
Sbjct:  518 ENIYPAELEDFFLKHPQVQEAQVVGVKDERMGEEICACIRLKSGETTTAEEIKAFCKGKISHFKIPRYIVFVEGYPLTISGKIQKFKLREQ 608

[Close]

BLAST of GT620683 vs. SwissProt
Match: ACSF2_MACFA (Acyl-CoA synthetase family member 2, mitochondrial OS=Macaca fascicularis GN=ACSF2 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.853e-14
Identity = 40/91 (43.96%), Postives = 57/91 (62.64%), Query Frame = -2

Query:  175 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEK 444
            ENI   ELE   + HP V E  VVG  D+  GE  CA ++LK+G   T EE+  FC+ ++ HF  PR +VF ++ P T++GK QK+ L+E+
Sbjct:  521 ENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTAEEMKAFCKGKISHFKIPRYIVFVTNYPLTTSGKIQKFKLREQ 611

[Close]

BLAST of GT620683 vs. SwissProt
Match: ACSF2_PONAB (Acyl-CoA synthetase family member 2, mitochondrial OS=Pongo abelii GN=ACSF2 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.727e-14
Identity = 41/91 (45.05%), Postives = 56/91 (61.54%), Query Frame = -2

Query:  175 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEK 444
            ENI   ELE   + HP V E  VVG  D+  GE  CA ++LK+G   T EEI  FC+ ++ HF  PR +VF ++ P T +GK QK+ L+E+
Sbjct:  518 ENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHFKIPRYIVFVTNYPLTISGKIQKFKLREQ 608

[Close]

BLAST of GT620683 vs. SwissProt
Match: MENE_BACWK (2-succinylbenzoate--CoA ligase OS=Bacillus weihenstephanensis (strain KBAB4) GN=menE PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.357e-14
Identity = 42/95 (44.21%), Postives = 60/95 (63.16%), Query Frame = -2

Query:  163 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEKAKAM 444
            ENI   ++E V+  HPAVVEA VVG  DE WG+ P AFV +K G + TEEEII+FC E+L  +  P+   F  +LP+ ++ K  +  L++  + M
Sbjct:  389 ENIYPAQIEEVLLSHPAVVEAGVVGMADESWGQVPAAFV-VKSG-DVTEEEIIRFCEEKLAKYKVPKKACFLEELPRNASKKLLRRELRKLVEEM 481

[Close]

BLAST of GT620683 vs. SwissProt
Match: ACSF2_HUMAN (Acyl-CoA synthetase family member 2, mitochondrial OS=Homo sapiens GN=ACSF2 PE=1 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 8.302e-14
Identity = 40/91 (43.96%), Postives = 56/91 (61.54%), Query Frame = -2

Query:  175 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEK 444
            ENI   ELE   + HP V E  VVG  D+  GE  CA ++LK+G   T EEI  FC+ ++ HF  P+ +VF ++ P T +GK QK+ L+E+
Sbjct:  518 ENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHFKIPKYIVFVTNYPLTISGKIQKFKLREQ 608

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT1G20560.2 (| Symbols: AAE1 | acyl activating enzyme 1 | chr1:7119927-7121363 REVERSE LENGTH=478)

HSP 1 Score: 147.517 bits (371), Expect = 6.636e-36
Identity = 70/116 (60.34%), Postives = 89/116 (76.72%), Query Frame = -2

Query:  163 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAM 510
            H  GY  +E     + I I   ENIS+IE+E  ++ HP V+EAAVV RPDEYWGET CAFVKLK+G  A+ EE+I +CR+RLPH+MAPR++VF DLPKTSTGK QK++L+ KAKA+
Sbjct:  354 HPDGY--IELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 467

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT1G20560.1 (| Symbols: AAE1 | acyl activating enzyme 1 | chr1:7119927-7121730 REVERSE LENGTH=556)

HSP 1 Score: 147.517 bits (371), Expect = 6.636e-36
Identity = 70/116 (60.34%), Postives = 89/116 (76.72%), Query Frame = -2

Query:  163 HTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAM 510
            H  GY  +E     + I I   ENIS+IE+E  ++ HP V+EAAVV RPDEYWGET CAFVKLK+G  A+ EE+I +CR+RLPH+MAPR++VF DLPKTSTGK QK++L+ KAKA+
Sbjct:  432 HPDGY--IELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 545

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT2G17650.1 (| Symbols: | AMP-dependent synthetase and ligase family protein | chr2:7671041-7672936 FORWARD LENGTH=603)

HSP 1 Score: 139.428 bits (350), Expect = 1.807e-33
Identity = 66/115 (57.39%), Postives = 88/115 (76.52%), Query Frame = -2

Query:  160 GYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATE-EEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 501
            GY  ++    L+ + I   ENIS++E+E V+  H AV+EAAVV RPD +WG+TPC FVKLKEG++  + EEII FCR+ LPH+MAP+T+VF D+PKTSTGK QKY+L++KA  MG
Sbjct:  489 GYIEIKDR--LKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEMG 601

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT3G16910.1 (| Symbols: AAE7, ACN1 | acyl-activating enzyme 7 | chr3:5773231-5775411 REVERSE LENGTH=569)

HSP 1 Score: 124.79 bits (312), Expect = 4.606e-29
Identity = 59/100 (59.00%), Postives = 76/100 (76.00%), Query Frame = -2

Query:  160 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGY-----NATEEEIIKFCRERLPHFMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 444
            ENIS++E+E V+Y HPAV+EA+VV RPDE W E+PCAFV LK  Y     N   ++I+KFCRE+LP +  P++VVF  LPKT+TGK QK+IL+ KAK MG
Sbjct:  463 ENISSVEVENVVYHHPAVLEASVVARPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGKIQKHILRTKAKEMG 562

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT5G16340.1 (| Symbols: | AMP-dependent synthetase and ligase family protein | chr5:5349255-5350907 REVERSE LENGTH=550)

HSP 1 Score: 123.25 bits (308), Expect = 1.340e-28
Identity = 61/121 (50.41%), Postives = 88/121 (72.73%), Query Frame = -2

Query:  160 MHTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNA--TEEEIIKFCRERLPHFMAPRTVVFSD-LPKTSTGKTQKYILKEKAKAMG 513
            +H+ GY  ++     + I I   EN+S++E+E V+Y  PAV E AVV RPDE+WGETPCAFV LK G++   TEEE++++CR+++P +M P+TV F D LPK+STGK  K++L++ AK MG
Sbjct:  427 IHSDGYLEIKDR--SKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFVLRDIAKKMG 545

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT5G16370.1 (| Symbols: AAE5 | acyl activating enzyme 5 | chr5:5356823-5358481 REVERSE LENGTH=552)

HSP 1 Score: 120.553 bits (301), Expect = 8.687e-28
Identity = 62/121 (51.24%), Postives = 85/121 (70.25%), Query Frame = -2

Query:  160 MHTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEG--YNATEEEIIKFCRERLPHFMAPRTVVFSD-LPKTSTGKTQKYILKEKAKAMG 513
            +H+ GY  ++     + I I   EN+S++E+E V+Y +PAV E AVV RPD +WGETPCAFV LK G     TE E+I++CR+++P +M P+TV F D LPKTSTGK  K++L+E AK MG
Sbjct:  427 IHSDGYLEIKDR--SKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFVLREIAKKMG 545

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT1G75960.1 (| Symbols: | AMP-dependent synthetase and ligase family protein | chr1:28518187-28519821 FORWARD LENGTH=544)

HSP 1 Score: 118.627 bits (296), Expect = 3.301e-27
Identity = 62/120 (51.67%), Postives = 85/120 (70.83%), Query Frame = -2

Query:  163 MHTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGY--NATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEKAKAM 513
            +H  GY  ++     + + I   EN+S++E+E V+Y +PAV EAAVV RPDE+WGETPCAFV LK G     T++EII++C+ ++P +MAP+TV F  +LPKTSTGK  K +LKE AK M
Sbjct:  427 IHGDGYLEIKDR--SKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKSLLKEIAKNM 544

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT1G77240.1 (| Symbols: | AMP-dependent synthetase and ligase family protein | chr1:29017958-29019595 REVERSE LENGTH=545)

HSP 1 Score: 116.701 bits (291), Expect = 1.254e-26
Identity = 62/119 (52.10%), Postives = 83/119 (69.75%), Query Frame = -2

Query:  163 MHTYGYDRVEQN*SLEKIDIE*AENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLK-EGYNATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEKAKAM 513
            MH  GY  V+     + + I   ENIS+ ELE V+Y +PA+ EAAVV +PD+ WGETPCAFV LK    + TE EI +FC+ +LP +M PR VVF  +LPKTSTGK QK++L++ AK++
Sbjct:  428 MHPDGYLEVKDR--SKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPDKMWGETPCAFVSLKYHDGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQKFLLRQMAKSL 544

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT1G76290.1 (| Symbols: | AMP-dependent synthetase and ligase family protein | chr1:28623443-28625408 REVERSE LENGTH=546)

HSP 1 Score: 115.161 bits (287), Expect = 3.650e-26
Identity = 57/98 (58.16%), Postives = 74/98 (75.51%), Query Frame = -2

Query:  160 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYNATEEEIIKFCRERLPH---FMAPRTVVFSDLPKTSTGKTQKYILKEKAKAMG 444
            E + + E+EG++Y HPAV +A VVGRPDE  GE+ CAFVKLKEG  A EEEII+FC+ +L +    M P+TVVFSD+PKT TGK +K +L++ AK MG
Sbjct:  441 EIVGSKEIEGILYSHPAVYDAGVVGRPDETLGESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNVLRKMAKDMG 538

[Close]

BLAST of GT620683 vs. TAIR peptide
Match: AT1G21540.1 (| Symbols: | AMP-dependent synthetase and ligase family protein | chr1:7548758-7550521 REVERSE LENGTH=550)

HSP 1 Score: 112.849 bits (281), Expect = 1.811e-25
Identity = 56/99 (56.57%), Postives = 73/99 (73.74%), Query Frame = -2

Query:  163 ENISTIELEGVIYGHPAVVEAAVVGRPDEYWGETPCAFVKLKEGYN----ATEEEIIKFCRERLPHFMAPRTVVF-SDLPKTSTGKTQKYILKEKAKAM 444
            ENIS+ E+E V+Y +P V EAAVV +PD+ WGETPCAFV LK   N     TE EI +FC+ RLP +M PR V+F  +LPKTSTGK QK++L++ AK++
Sbjct:  451 ENISSAEVETVLYTNPVVKEAAVVAKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKIQKFLLRQMAKSL 549

The following BLAST results are available for this feature:

BLAST of GT620683 vs. TrEMBL
BLAST of GT620683 vs. SwissProt
BLAST of GT620683 vs. TAIR peptide

BLAST of GT620683 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8H8C9_ORYSJ	6.979e-40	69.23	AMP-dependent synthetase and ligase family protein...	[more]
Q0DVE7_ORYSJ	6.979e-40	69.23	Os03g0133600 protein (Fragment) OS=Oryza sativa su...	[more]
A2XC55_ORYSI	6.979e-40	69.23	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B9H5H2_POPTR	4.524e-39	67.52	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5WZ16_SORBI	2.245e-38	66.67	Putative uncharacterized protein Sb01g048050 OS=So...	[more]
B9S6S7_RICCO	2.245e-38	64.96	AMP dependent ligase, putative OS=Ricinus communis...	[more]
D7U6N8_VITVI	3.242e-37	62.39	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5WZU1_SORBI	3.242e-37	65.81	Putative uncharacterized protein Sb01g048090 OS=So...	[more]
C4J0R9_MAIZE	3.242e-37	64.10	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C0PGQ6_MAIZE	3.242e-37	64.10	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of GT620683 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ACSF2_DANRE	3.982e-16	38.94	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
YNGI_BACSU	3.371e-15	39.47	Putative acyl-CoA synthetase yngI OS=Bacillus subt...	[more]
ACSF2_RAT	5.749e-15	47.25	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
MENE_GEOSW	7.509e-15	42.22	2-succinylbenzoate--CoA ligase OS=Geobacillus sp. ...	[more]
ACSF2_BOVIN	1.281e-14	46.15	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
ACSF2_MOUSE	2.853e-14	47.25	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
ACSF2_MACFA	2.853e-14	43.96	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
ACSF2_PONAB	3.727e-14	45.05	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
MENE_BACWK	6.357e-14	44.21	2-succinylbenzoate--CoA ligase OS=Bacillus weihens...	[more]
ACSF2_HUMAN	8.302e-14	43.96	Acyl-CoA synthetase family member 2, mitochondrial...	[more]

BLAST of GT620683 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G20560.2	6.636e-36	60.34	\| Symbols: AAE1 \| acyl activating enzyme 1 \| chr1:...	[more]
AT1G20560.1	6.636e-36	60.34	\| Symbols: AAE1 \| acyl activating enzyme 1 \| chr1:...	[more]
AT2G17650.1	1.807e-33	57.39	\| Symbols: \| AMP-dependent synthetase and ligase ...	[more]
AT3G16910.1	4.606e-29	59.00	\| Symbols: AAE7, ACN1 \| acyl-activating enzyme 7 \|...	[more]
AT5G16340.1	1.340e-28	50.41	\| Symbols: \| AMP-dependent synthetase and ligase ...	[more]
AT5G16370.1	8.687e-28	51.24	\| Symbols: AAE5 \| acyl activating enzyme 5 \| chr5:...	[more]
AT1G75960.1	3.301e-27	51.67	\| Symbols: \| AMP-dependent synthetase and ligase ...	[more]
AT1G77240.1	1.254e-26	52.10	\| Symbols: \| AMP-dependent synthetase and ligase ...	[more]
AT1G76290.1	3.650e-26	58.16	\| Symbols: \| AMP-dependent synthetase and ligase ...	[more]
AT1G21540.1	1.811e-25	56.57	\| Symbols: \| AMP-dependent synthetase and ligase ...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 79..90 score
None	No IPR available	GENE3D	3.30.300.30	no description	coord: 2..94 score: 1.7
None	No IPR available	PANTHER	PTHR11968	ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBER	coord: 2..95 score: 6.4
None	No IPR available	PANTHER	PTHR11968:SF26	ADENOSINE MONOPHOSPHATE BINDING PROTEIN	coord: 2..95 score: 6.4
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 2..95 score: 4.3

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620683 ID=GT620683; Name=GT620683; organism=Lens culinaris; type=EST; length=706bp
CGAATTGAAGTTTCTTTATTCAAATAAACAAATCTTACATGCATATGATA
AGTACTAACATTACATCACACCATGACATTAAAGAATATTATTACATTCA
GTTTTATTTTCTTTAGAAATGCATTACAATCTGCTGCTGTTCTTTTTCTT
AGACAAGCTTCCCATAGCCTTTGCTTTCTCCTTCAAAATATATTTCTGTG
TCTTGCCAGTTGATGTCTTTGGCAGATCAGAAAACACGACTGTTCGAGGA
GCCATAAAATGAGGCAAACGCTCCCGACAAAATTTGATTATCTCTTCTTC
TGTTGCATTGTACCCTTCCTTCAGCTTCACAAATGCACAGGGTGTCTCTC
CCCAATATTCATCAGGTCTCCCCACAACAGCAGCTTCAACAACTGCTGGA
TGACCATAAATCACACCTTCCAACTCAATTGTGCTAATGTTTTCGGCTTA
CTCAATATCTATCTTCTCCAACGACTAATTTTGTTCAACTCTGTCGTAAC
CATAAGTGTGCATCTAATGATTTGTTCGTAGACTTTTAGTAATTGTAATA
GCATGTAAAAACGTACATGGAGATGATTGTCCTATGCAGGTGAAGCTATT
TTGATAGCTAGTATGCAATATCCAAGTTTGTAGTGCAGTGCAGATTGTGC
GCCTGTTGCATGTGCAAAGCTTTTATTTTGTTCTTTAACTTTGGACGCCC
GTGACT

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