GT620834

EST Overview


Name	GT620834
Unique Name	GT620834
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	776

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT620834 vs. TrEMBL
Match: C6T0L4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 2.211e-56
Identity = 107/144 (74.31%), Postives = 120/144 (83.33%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV*HLD 553
            MVSFEMNDRKKI                    DKGLLAMGN+LFVSGVSLTIGLKSTMQFFMKRSNFKGT+SFG+GF ILI+GWPILGMIVESYGF+VLFSGFWPTL++FLQKIPVLGW+ QQP++RSL DRY+G+RVPV H+D
Sbjct:    1 MVSFEMNDRKKIGLGLTGFGIFFSFLGLIFFFDKGLLAMGNVLFVSGVSLTIGLKSTMQFFMKRSNFKGTISFGIGFFILILGWPILGMIVESYGFIVLFSGFWPTLAVFLQKIPVLGWLFQQPFVRSLLDRYRGRRVPVKHVD 144

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BLAST of GT620834 vs. TrEMBL
Match: B7FGH0_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 4.925e-56
Identity = 105/140 (75.00%), Postives = 119/140 (85.00%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M+SFEMNDRKKI                    DKGLLAMGN+LFVSGVSLTIGLKSTMQFFMKRSNFKGT+SFG+GFLILI+GWPILGMI+E+YGF+VLFSGFWPTL++FLQKIPVLGW++QQP+IRS FDRY+GKRVPV
Sbjct:    1 MLSFEMNDRKKIGLGLTGFGIFFSFLGIVFFFDKGLLAMGNVLFVSGVSLTIGLKSTMQFFMKRSNFKGTISFGIGFLILILGWPILGMIIEAYGFIVLFSGFWPTLAVFLQKIPVLGWVIQQPFIRSFFDRYRGKRVPV 140

[Close]

BLAST of GT620834 vs. TrEMBL
Match: C6T5P0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 3.192e-55
Identity = 105/140 (75.00%), Postives = 118/140 (84.29%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            MVSFEMNDRKKI                    DKGLLAMGN+LFVSGVS+TIGLKSTMQFFMKRSNFKGT+SFGVGF ILIMGWPI+GMIVE+YGF+VLFSGFWPTL++FLQKIPVLGW+ QQP++RSLFDRY+GKR+PV
Sbjct:    1 MVSFEMNDRKKIGLGLTGFGIFFSFLGIIFFFDKGLLAMGNVLFVSGVSITIGLKSTMQFFMKRSNFKGTISFGVGFFILIMGWPIVGMIVEAYGFVVLFSGFWPTLAVFLQKIPVLGWLFQQPFVRSLFDRYRGKRMPV 140

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BLAST of GT620834 vs. TrEMBL
Match: C6SX17_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 1.584e-54
Identity = 105/140 (75.00%), Postives = 117/140 (83.57%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            MVSFEMND+KKI                    DKGLL MGNILFVSGVS+TIGLKSTMQFF+KRSNFKGT+SFGVGF ILIMGWPILGMIVE+YGF+VLFSGFWPTL++FLQKIPVLGW+ QQP+IRSLFDRY+GKR+PV
Sbjct:    1 MVSFEMNDQKKIGLGLTGFGIFFSFLGIIFFFDKGLLVMGNILFVSGVSITIGLKSTMQFFVKRSNFKGTISFGVGFFILIMGWPILGMIVEAYGFIVLFSGFWPTLAVFLQKIPVLGWLFQQPFIRSLFDRYRGKRMPV 140

[Close]

BLAST of GT620834 vs. TrEMBL
Match: Q9SCL4_ARATH (At3g49420 OS=Arabidopsis thaliana GN=At3g49420/T9C5.20 PE=2 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 2.529e-52
Identity = 99/140 (70.71%), Postives = 113/140 (80.71%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M+SFEMNDRKKI                    DKGLLAMGNILF+SGVSLTIG KSTMQFFMKR N+KGT+SFGVGF  +I+GWPILGM++E+YGF VLFSGFWPTL++F QKIPVLGWI+QQPYIRS FD+Y+GKRVPV
Sbjct:    1 MMSFEMNDRKKIGLGLTGFGVFFSFLGIVFVFDKGLLAMGNILFISGVSLTIGFKSTMQFFMKRQNYKGTISFGVGFFFVIIGWPILGMMLETYGFFVLFSGFWPTLAVFAQKIPVLGWIIQQPYIRSFFDKYRGKRVPV 140

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BLAST of GT620834 vs. TrEMBL
Match: D7M722_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_486933 PE=4 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 3.303e-52
Identity = 98/140 (70.00%), Postives = 113/140 (80.71%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M+SFEMNDRKKI                    DKGLLAMGNILF+SGVSLTIG KSTMQFFMKR N+KGT+SFGVGF  +I+GWPILGM++E+YGF VLFSGFWPTL++F QKIP+LGWI+QQPYIRS FD+Y+GKRVPV
Sbjct:    1 MMSFEMNDRKKIGLGLTGFGVFFSFLGIVFVFDKGLLAMGNILFISGVSLTIGFKSTMQFFMKRQNYKGTISFGVGFFFVIIGWPILGMMLETYGFFVLFSGFWPTLAVFAQKIPILGWIIQQPYIRSFFDKYRGKRVPV 140

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BLAST of GT620834 vs. TrEMBL
Match: D7TTH3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_12.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00013243001 PE=4 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 2.141e-51
Identity = 96/140 (68.57%), Postives = 114/140 (81.43%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            MVSFEMNDRKKI                    DKGLLAMGNILF+SGV+LTIGLKST+QFFMKR N+KGT+SFGVGF  +I+GWPILGM++E+YGF+VLFSGFWPTL++FLQKIP LGW+ QQP++RS FD+Y+GKRVPV
Sbjct:    1 MVSFEMNDRKKIGLGLTAFGVFFSFLGIVFLFDKGLLAMGNILFISGVTLTIGLKSTVQFFMKRQNYKGTISFGVGFFFVIIGWPILGMMLEAYGFIVLFSGFWPTLAVFLQKIPFLGWVFQQPFVRSFFDKYRGKRVPV 140

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BLAST of GT620834 vs. TrEMBL
Match: B9MXZ3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_741425 PE=4 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 6.230e-51
Identity = 93/140 (66.43%), Postives = 112/140 (80.00%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            MVSFE+NDRKKI                    DKG LAMGNILF+SGVSLTIG KSTMQFFMKR NFKGT+SFG GF  +++GWP++GMI+E+YGF+VLFSGFWPTL++F+QKIP+LGW+ QQP++RS FDRY+GKRVPV
Sbjct:    1 MVSFELNDRKKIGLGLTGFGIFFSFLGIVFFFDKGFLAMGNILFISGVSLTIGPKSTMQFFMKRQNFKGTISFGAGFFFVVIGWPVIGMILEAYGFIVLFSGFWPTLAVFIQKIPILGWVFQQPFVRSFFDRYRGKRVPV 140

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BLAST of GT620834 vs. TrEMBL
Match: B9HHD3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_819718 PE=4 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 8.136e-51
Identity = 93/140 (66.43%), Postives = 112/140 (80.00%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            MVSFEMNDRKKI                    DKGLLAMGNILF+SGVSLTIG KSTMQFFMKR NFKGT+SFG GF  +++GWP++GMI+E+YGF+VLFSGFWPTL++F+Q+IP+ GW+ QQP++RS FDRY+GKRVPV
Sbjct:    1 MVSFEMNDRKKIGLGLTGFGIFFSFLGIVFFFDKGLLAMGNILFISGVSLTIGPKSTMQFFMKRQNFKGTISFGAGFFFVVIGWPVIGMILEAYGFIVLFSGFWPTLAVFVQRIPIFGWVFQQPFVRSFFDRYRGKRVPV 140

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BLAST of GT620834 vs. TrEMBL
Match: Q6NMM1_ARATH (At3g03180 OS=Arabidopsis thaliana GN=At3g03180 PE=2 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 1.216e-46
Identity = 87/140 (62.14%), Postives = 109/140 (77.86%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M S EMND KKI                    DKGL+AMGNILF++GV+LTIG+   +QFF KR NFKGT+SFG+GFL+++ GWPI G+++ESYGFLVLFSGFWPTL++FLQ+IP+LGW++QQPYIRSL DRY+G+RVPV
Sbjct:    1 MASLEMNDLKKIGLGLTGFGVFFTFLGVIFVFDKGLIAMGNILFLAGVTLTIGINPAIQFFTKRQNFKGTISFGLGFLLVVFGWPIFGLLLESYGFLVLFSGFWPTLAVFLQRIPLLGWLLQQPYIRSLLDRYRGRRVPV 140

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BLAST of GT620834 vs. SwissProt
Match: GOT1B_MOUSE (Vesicle transport protein GOT1B OS=Mus musculus GN=Golt1b PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 5.715e-22
Identity = 47/100 (47.00%), Postives = 71/100 (71.00%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDR 517
            DK LLA+GN+LFV+G++  IGL+ T +FF +R   K T  F  G  ++++GWP++GMI E YGF +LF GF+P +  F++++PVLG ++  P IRS  D+
Sbjct:   31 DKALLAIGNVLFVAGLAFVIGLERTFRFFFQRHKVKATGFFLGGVFVVLIGWPLIGMIFEIYGFFLLFRGFFPVVVGFIRRVPVLGSLLNLPGIRSFVDK 130

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BLAST of GT620834 vs. SwissProt
Match: GOT1B_HUMAN (Vesicle transport protein GOT1B OS=Homo sapiens GN=GOLT1B PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 9.748e-22
Identity = 46/100 (46.00%), Postives = 71/100 (71.00%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDR 517
            DK LLA+GN+LFV+G++  IGL+ T +FF ++   K T  F  G  ++++GWP++GMI E YGF +LF GF+P +  F++++PVLG ++  P IRS  D+
Sbjct:   31 DKALLAIGNVLFVAGLAFVIGLERTFRFFFQKHKMKATGFFLGGVFVVLIGWPLIGMIFEIYGFFLLFRGFFPVVVGFIRRVPVLGSLLNLPGIRSFVDK 130

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BLAST of GT620834 vs. SwissProt
Match: GOT1B_BOVIN (Vesicle transport protein GOT1B OS=Bos taurus GN=GOLT1B PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 9.748e-22
Identity = 46/100 (46.00%), Postives = 71/100 (71.00%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDR 517
            DK LLA+GN+LFV+G++  IGL+ T +FF ++   K T  F  G  ++++GWP++GMI E YGF +LF GF+P +  F++++PVLG ++  P IRS  D+
Sbjct:   31 DKALLAIGNVLFVAGLAFVIGLERTFRFFFQKHKMKATGFFLGGVFVVLIGWPLIGMIFEIYGFFLLFRGFFPVVVGFIRRVPVLGSLLNLPGIRSFVDK 130

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BLAST of GT620834 vs. SwissProt
Match: GOT1_CAEEL (Probable Golgi transport protein 1 OS=Caenorhabditis elegans GN=F41C3.4 PE=2 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 1.009e-18
Identity = 47/132 (35.61%), Postives = 74/132 (56.06%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDR 517
            M++FE++  K+I                    D  LLA+GN+LF+ G++  IG++ T+ FF +    KG++ F  G L+++ G+P+ GMI E +GF+VLF GF P +   L+ IP +  I   P IR + DR
Sbjct:    1 MMNFEVSTTKQIGVGLTTFGFFFIFLGVLMFLDSALLAIGNLLFIVGITFIIGVQRTLVFFFEFRKLKGSILFFGGILVVLFGYPLFGMIAECWGFIVLFGGFLPGIVNLLRSIPGISTITYLPGIRQVLDR 132

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BLAST of GT620834 vs. SwissProt
Match: GOT1A_BOVIN (Vesicle transport protein GOT1A OS=Bos taurus GN=GOLT1A PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.538e-18
Identity = 47/103 (45.63%), Postives = 65/103 (63.11%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKG 526
            D  LLA GN+LF++G+SL IGL+ T  FF +R  FKGT  F  G +I+++ WP+LGM +E+YGF  LF GF+P    F      LG     P++ +LF R +G
Sbjct:   31 DSVLLAFGNLLFLTGLSLIIGLRRTFSFFFQRHKFKGTSFFLGGVVIVLLRWPLLGMCLETYGFFSLFRGFFPVAFGF------LGSASNIPFLSALFQRLQG 127

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BLAST of GT620834 vs. SwissProt
Match: GOT1A_MOUSE (Vesicle transport protein GOT1A OS=Mus musculus GN=Golt1a PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.457e-17
Identity = 45/103 (43.69%), Postives = 66/103 (64.08%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKG 526
            D  LLA GN+LF++G+SL IGL+ T  FF +R   KGT  F  G  I+++ WP+LGM++E+YGF+ LF GF+P +  F      LG     P++ +LF + +G
Sbjct:   31 DSVLLAFGNLLFLTGLSLIIGLRRTFAFFFQRHKLKGTSFFLGGVAIVLLRWPLLGMLLEAYGFISLFKGFFPVVFGF------LGSAFNIPFLSTLFQKLQG 127

[Close]

BLAST of GT620834 vs. SwissProt
Match: GOT1A_HUMAN (Vesicle transport protein GOT1A OS=Homo sapiens GN=GOLT1A PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 9.441e-17
Identity = 45/103 (43.69%), Postives = 64/103 (62.14%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKG 526
            D  LLA GN+LF++G+SL IGL+ T  FF +R   KGT     G +I+++ WP+LGM +E+YGF  LF GF+P    F      LG +   P++ +LF R +G
Sbjct:   31 DSVLLAFGNLLFLTGLSLIIGLRKTFWFFFQRHKLKGTSFLLGGVVIVLLRWPLLGMFLETYGFFSLFKGFFPVAFGF------LGNVCNIPFLGALFRRLQG 127

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BLAST of GT620834 vs. SwissProt
Match: GOT1_DICDI (Protein transport protein got1 homolog OS=Dictyostelium discoideum GN=golt1 PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.103e-16
Identity = 38/101 (37.62%), Postives = 66/101 (65.35%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIM-GWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDR 517
            D+ LLA+GN+L VSG+ L +GL+ T +FF ++   KGT+ F  G ++L++  W  +GM++E +GF+ LF   +P +   L+K+P++G I+  P +  L  +
Sbjct:   29 DRNLLALGNLLLVSGIVLILGLQKTTKFFAQKKKIKGTILFFFGIVVLLVTRWTFVGMVIEIFGFVNLFGDAFPIVISILRKLPIIGNILNHPLVNRLLQK 129

[Close]

BLAST of GT620834 vs. SwissProt
Match: GOT1_YEAST (Protein transport protein GOT1 OS=Saccharomyces cerevisiae GN=GOT1 PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.747e-16
Identity = 42/106 (39.62%), Postives = 66/106 (62.26%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRV 535
            D+ LLA+GNILF+ GV L IG + T  FF + +  +G++ F VG  ++++ W  LG I+ES G + LF  F+  +  FL+ +P++G I+  P I  + D+  G RV
Sbjct:   30 DRALLALGNILFLIGVFLIIGSQKTYIFFTRPNKRRGSLFFLVGAFLILLKWTFLGFIIESLGIIGLFGDFFGVIVQFLRSMPIIGPILSHPAIAPIVDKLAGVRV 135

[Close]

BLAST of GT620834 vs. SwissProt
Match: GOT1_SCHPO (Protein transport protein got1 OS=Schizosaccharomyces pombe GN=got1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.194e-11
Identity = 35/97 (36.08%), Postives = 55/97 (56.70%), Query Frame = 2

Query:  218 DKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSL 508
            D  LL++GN+L V G  +  G   ++ FF+++    G++SF  G L+ +  +PI+G  VE  GF  LF  F+P +  FL+ +P +G     PYI  L
Sbjct:   30 DGPLLSLGNLLLVFGFFMIAGFSKSVSFFLRKDRMLGSISFFSGLLLTLFHFPIIGFFVECLGFFNLFKVFYPLIISFLRTVPYIG-----PYIDRL 121

[Close]

BLAST of GT620834 vs. TAIR peptide
Match: AT5G01430.2 (| Symbols: | Got1/Sft2-like vescicle transport protein family | chr5:176797-178010 REVERSE LENGTH=140)

HSP 1 Score: 209.534 bits (532), Expect = 1.643e-54
Identity = 99/140 (70.71%), Postives = 113/140 (80.71%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M+SFEMNDRKKI                    DKGLLAMGNILF+SGVSLTIG KSTMQFFMKR N+KGT+SFGVGF  +I+GWPILGM++E+YGF VLFSGFWPTL++F QKIPVLGWI+QQPYIRS FD+Y+GKRVPV
Sbjct:    1 MMSFEMNDRKKIGLGLTGFGVFFSFLGIVFVFDKGLLAMGNILFISGVSLTIGFKSTMQFFMKRQNYKGTISFGVGFFFVIIGWPILGMMLETYGFFVLFSGFWPTLAVFAQKIPVLGWIIQQPYIRSFFDKYRGKRVPV 140

[Close]

BLAST of GT620834 vs. TAIR peptide
Match: AT5G01430.1 (| Symbols: | Got1/Sft2-like vescicle transport protein family | chr5:176797-178010 REVERSE LENGTH=140)

HSP 1 Score: 209.534 bits (532), Expect = 1.643e-54
Identity = 99/140 (70.71%), Postives = 113/140 (80.71%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M+SFEMNDRKKI                    DKGLLAMGNILF+SGVSLTIG KSTMQFFMKR N+KGT+SFGVGF  +I+GWPILGM++E+YGF VLFSGFWPTL++F QKIPVLGWI+QQPYIRS FD+Y+GKRVPV
Sbjct:    1 MMSFEMNDRKKIGLGLTGFGVFFSFLGIVFVFDKGLLAMGNILFISGVSLTIGFKSTMQFFMKRQNYKGTISFGVGFFFVIIGWPILGMMLETYGFFVLFSGFWPTLAVFAQKIPVLGWIIQQPYIRSFFDKYRGKRVPV 140

[Close]

BLAST of GT620834 vs. TAIR peptide
Match: AT3G49420.1 (| Symbols: | Got1/Sft2-like vescicle transport protein family | chr3:18329855-18331070 FORWARD LENGTH=140)

HSP 1 Score: 209.534 bits (532), Expect = 1.643e-54
Identity = 99/140 (70.71%), Postives = 113/140 (80.71%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M+SFEMNDRKKI                    DKGLLAMGNILF+SGVSLTIG KSTMQFFMKR N+KGT+SFGVGF  +I+GWPILGM++E+YGF VLFSGFWPTL++F QKIPVLGWI+QQPYIRS FD+Y+GKRVPV
Sbjct:    1 MMSFEMNDRKKIGLGLTGFGVFFSFLGIVFVFDKGLLAMGNILFISGVSLTIGFKSTMQFFMKRQNYKGTISFGVGFFFVIIGWPILGMMLETYGFFVLFSGFWPTLAVFAQKIPVLGWIIQQPYIRSFFDKYRGKRVPV 140

[Close]

BLAST of GT620834 vs. TAIR peptide
Match: AT3G03180.1 (| Symbols: | Got1/Sft2-like vescicle transport protein family | chr3:733531-734421 FORWARD LENGTH=140)

HSP 1 Score: 190.66 bits (483), Expect = 7.899e-49
Identity = 87/140 (62.14%), Postives = 109/140 (77.86%), Query Frame = 2

Query:  122 MVSFEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQPYIRSLFDRYKGKRVPV 541
            M S EMND KKI                    DKGL+AMGNILF++GV+LTIG+   +QFF KR NFKGT+SFG+GFL+++ GWPI G+++ESYGFLVLFSGFWPTL++FLQ+IP+LGW++QQPYIRSL DRY+G+RVPV
Sbjct:    1 MASLEMNDLKKIGLGLTGFGVFFTFLGVIFVFDKGLIAMGNILFLAGVTLTIGINPAIQFFTKRQNFKGTISFGLGFLLVVFGWPIFGLLLESYGFLVLFSGFWPTLAVFLQRIPLLGWLLQQPYIRSLLDRYRGRRVPV 140

[Close]

BLAST of GT620834 vs. TAIR peptide
Match: AT1G05785.2 (| Symbols: | Got1/Sft2-like vescicle transport protein family | chr1:1730981-1732406 FORWARD LENGTH=135)

HSP 1 Score: 105.916 bits (263), Expect = 2.559e-23
Identity = 46/121 (38.02%), Postives = 74/121 (61.16%), Query Frame = 2

Query:  131 FEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQP 493
            +E+ ++KK+                    D+GLLA+GN+ ++ GV L +G +ST + F   +N +GT+ F +G  ++ + WPI+G+I+E YG +VLF GFW T+  FL +IP +GWI+Q P
Sbjct:    3 YEITEQKKVGLGLIGFGLSFTFLGVILYFDRGLLALGNLFWLIGVGLLLGWQSTWRVFTNVNNLRGTICFVLGLFLIFVRWPIIGIILEIYGVIVLFGGFWSTVKAFLSQIPFVGWIIQYP 123

[Close]

BLAST of GT620834 vs. TAIR peptide
Match: AT1G05785.1 (| Symbols: | Got1/Sft2-like vescicle transport protein family | chr1:1730981-1732406 FORWARD LENGTH=135)

HSP 1 Score: 105.916 bits (263), Expect = 2.559e-23
Identity = 46/121 (38.02%), Postives = 74/121 (61.16%), Query Frame = 2

Query:  131 FEMNDRKKIXXXXXXXXXXXXXXXXXXXXDKGLLAMGNILFVSGVSLTIGLKSTMQFFMKRSNFKGTVSFGVGFLILIMGWPILGMIVESYGFLVLFSGFWPTLSLFLQKIPVLGWIVQQP 493
            +E+ ++KK+                    D+GLLA+GN+ ++ GV L +G +ST + F   +N +GT+ F +G  ++ + WPI+G+I+E YG +VLF GFW T+  FL +IP +GWI+Q P
Sbjct:    3 YEITEQKKVGLGLIGFGLSFTFLGVILYFDRGLLALGNLFWLIGVGLLLGWQSTWRVFTNVNNLRGTICFVLGLFLIFVRWPIIGIILEIYGVIVLFGGFWSTVKAFLSQIPFVGWIIQYP 123

The following BLAST results are available for this feature:

BLAST of GT620834 vs. TrEMBL
BLAST of GT620834 vs. SwissProt
BLAST of GT620834 vs. TAIR peptide

BLAST of GT620834 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T0L4_SOYBN	2.211e-56	74.31	Putative uncharacterized protein OS=Glycine max PE...	[more]
B7FGH0_MEDTR	4.925e-56	75.00	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6T5P0_SOYBN	3.192e-55	75.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SX17_SOYBN	1.584e-54	75.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q9SCL4_ARATH	2.529e-52	70.71	At3g49420 OS=Arabidopsis thaliana GN=At3g49420/T9C...	[more]
D7M722_ARALY	3.303e-52	70.00	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7TTH3_VITVI	2.141e-51	68.57	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9MXZ3_POPTR	6.230e-51	66.43	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HHD3_POPTR	8.136e-51	66.43	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q6NMM1_ARATH	1.216e-46	62.14	At3g03180 OS=Arabidopsis thaliana GN=At3g03180 PE=...	[more]

BLAST of GT620834 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GOT1B_MOUSE	5.715e-22	47.00	Vesicle transport protein GOT1B OS=Mus musculus GN...	[more]
GOT1B_HUMAN	9.748e-22	46.00	Vesicle transport protein GOT1B OS=Homo sapiens GN...	[more]
GOT1B_BOVIN	9.748e-22	46.00	Vesicle transport protein GOT1B OS=Bos taurus GN=G...	[more]
GOT1_CAEEL	1.009e-18	35.61	Probable Golgi transport protein 1 OS=Caenorhabdit...	[more]
GOT1A_BOVIN	6.538e-18	45.63	Vesicle transport protein GOT1A OS=Bos taurus GN=G...	[more]
GOT1A_MOUSE	1.457e-17	43.69	Vesicle transport protein GOT1A OS=Mus musculus GN...	[more]
GOT1A_HUMAN	9.441e-17	43.69	Vesicle transport protein GOT1A OS=Homo sapiens GN...	[more]
GOT1_DICDI	2.103e-16	37.62	Protein transport protein got1 homolog OS=Dictyost...	[more]
GOT1_YEAST	2.747e-16	39.62	Protein transport protein GOT1 OS=Saccharomyces ce...	[more]
GOT1_SCHPO	1.194e-11	36.08	Protein transport protein got1 OS=Schizosaccharomy...	[more]

BLAST of GT620834 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT5G01430.2	1.643e-54	70.71	\| Symbols: \| Got1/Sft2-like vescicle transport pr...	[more]
AT5G01430.1	1.643e-54	70.71	\| Symbols: \| Got1/Sft2-like vescicle transport pr...	[more]
AT3G49420.1	1.643e-54	70.71	\| Symbols: \| Got1/Sft2-like vescicle transport pr...	[more]
AT3G03180.1	7.899e-49	62.14	\| Symbols: \| Got1/Sft2-like vescicle transport pr...	[more]
AT1G05785.2	2.559e-23	38.02	\| Symbols: \| Got1/Sft2-like vescicle transport pr...	[more]
AT1G05785.1	2.559e-23	38.02	\| Symbols: \| Got1/Sft2-like vescicle transport pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007305	Vesicle transport protein, Got1/SFT2-like	PFAM	PF04178	Got1	coord: 23..141 score: 8.8
None	No IPR available	PANTHER	PTHR21493	CGI-141-RELATED/LIPASE CONTAINING PROTEIN	coord: 74..152 score: 1.7
None	No IPR available	SEG	seg	seg	coord: 25..44 score
None	No IPR available	SEG	seg	seg	coord: 6..19 score
None	No IPR available	SEG	seg	seg	coord: 69..82 score
None	No IPR available	SEG	seg	seg	coord: 67..80 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620834 ID=GT620834; Name=GT620834; organism=Lens culinaris; type=EST; length=776bp
TCACGCGCCTCTACACAGCCATTTCCATCTTCGTCAAACCCAAACCCCAA
GCAATCGCCACCACATCAAGGTGACTCAACCGTGAAAGCAAGCCTTTGAA
CATTTGAATTTTTGGTTTAAAATGGTTTCCTTTGAGATGAATGACCGCAA
GAAGATTGGATTGGGATTAACAGGCTTTGGTATATTTTTCTCATTCCTCG
GCGTTATATTTTTCTTTGACAAGGGTTTGCTAGCAATGGGAAATATCCTC
TTTGTTTCTGGAGTATCTCTGACCATTGGACTGAAATCCACCATGCAATT
CTTCATGAAACGTAGTAATTTCAAGGGGACAGTTTCATTTGGTGTAGGAT
TCTTGATTCTTATAATGGGATGGCCTATTTTGGGCATGATTGTGGAATCT
TACGGGTTCCTCGTGCTCTTCAGCGGCTTCTGGCCTACACTCTCTCTTTT
CTTGCAGAAGATTCCCGTTCTTGGTTGGATTGTTCAACAGCCATATATTC
GATCGTTGTTTGACCGCTATAAAGGCAAACGAGTGCCTGTGTAACACTTG
GATCCATGGAAACATTATGTAGCTGCTCACAAACGCATATGTAAGTCCAT
GTAAAGAAATTACTTGATACAAAGACCTAGCTCCAAATGGCTCTCATTTT
GATGGTAGAAAATTGTCATGATAGTCAAAAGAAGATGGAAAGGATAAAGG
TTTTGGTTTTCATTTTGCAACTCATTGCTTAGTCCATTTTCAACTCTCAT
TCGTTGTTAAACATTGAGTTGTCAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0016192	vesicle-mediated transport

Vocabulary: **INTERPRO**
Term	Definition
IPR007305	Vesicle_transpt_Got1/SFT2

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