GT621129

EST Overview


Name	GT621129
Unique Name	GT621129
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	840

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT621129 vs. TrEMBL
Match: B7FHC6_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 280.026 bits (715), Expect = 1.760e-73
Identity = 139/148 (93.92%), Postives = 143/148 (96.62%), Query Frame = 2

Query:   17 MLRIAKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            MLRIAK+LV TASFE P PA+RVLPRFYHERVVDHYDNPRNVGSFDKNDPT+GTGLVGAPACGDVMKLQIKVDE  GKI+DARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE
Sbjct:    1 MLRIAKKLVQTASFETPNPAIRVLPRFYHERVVDHYDNPRNVGSFDKNDPTVGTGLVGAPACGDVMKLQIKVDE-NGKIIDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 147

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BLAST of GT621129 vs. TrEMBL
Match: C6T2J7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 5.133e-65
Identity = 127/149 (85.23%), Postives = 136/149 (91.28%), Query Frame = 2

Query:   17 MLRI-AKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            MLR+ AKR++ T  F + +PA     R YHERVVDHYDNPRNVGSFDKNDP++GTGLVGAPACGDVMKLQIKVD+ TGKIVDARFKTFGCGSAIASSSVATEWVKG+QMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE
Sbjct:    1 MLRVGAKRVLETTLFGVRVPA-----RLYHERVVDHYDNPRNVGSFDKNDPSVGTGLVGAPACGDVMKLQIKVDDKTGKIVDARFKTFGCGSAIASSSVATEWVKGRQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 144

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BLAST of GT621129 vs. TrEMBL
Match: B8R1J5_EUCGR (Iron-sulfer cluster scaffold protein ISU1 OS=Eucalyptus grandis GN=ISU1 PE=2 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 2.157e-63
Identity = 130/151 (86.09%), Postives = 136/151 (90.07%), Query Frame = 2

Query:   17 MLRI-AKRLVGTASFEMPIPAVRVLP--RFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            MLR+ AK+L G A      PA RV+P  R YHERVVDHY+NPRNVGSFDKNDP +GTGLVGAPACGDVMKLQIKVDE TGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVL+IKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE
Sbjct:    1 MLRLGAKKLAGLAGAAAG-PA-RVVPPLRLYHERVVDHYNNPRNVGSFDKNDPAVGTGLVGAPACGDVMKLQIKVDEETGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLSIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 149

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BLAST of GT621129 vs. TrEMBL
Match: B9RB15_RICCO (Nitrogen fixation protein nifu, putative OS=Ricinus communis GN=RCOM_1510480 PE=4 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 3.679e-63
Identity = 125/149 (83.89%), Postives = 136/149 (91.28%), Query Frame = 2

Query:   17 MLR-IAKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            MLR ++KRL+G  S   P P V++L R YHE V+DH++NPRNVGSFDKNDP +GTGLVGAPACGDVMKLQIKVDE TG+IVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY+
Sbjct:    1 MLRLVSKRLLGQVS---PRP-VQILQRCYHENVIDHFNNPRNVGSFDKNDPAVGTGLVGAPACGDVMKLQIKVDEKTGEIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYQ 145

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BLAST of GT621129 vs. TrEMBL
Match: A9PEA4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_575170 PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 4.805e-63
Identity = 114/127 (89.76%), Postives = 125/127 (98.43%), Query Frame = 2

Query:   80 RVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            R+LPRFYHE+V+DH++NPRNVGSFDKNDP +GTGLVGAPACGDVMKLQIKVD+ TG+I+DARFKTFGCGSAIASSSVATEWVKGKQMEEV+TIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY+
Sbjct:   17 RILPRFYHEKVIDHFNNPRNVGSFDKNDPAVGTGLVGAPACGDVMKLQIKVDDTTGEIIDARFKTFGCGSAIASSSVATEWVKGKQMEEVMTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYQ 143

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BLAST of GT621129 vs. TrEMBL
Match: A9PDY0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834330 PE=2 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.826e-62
Identity = 113/127 (88.98%), Postives = 125/127 (98.43%), Query Frame = 2

Query:   80 RVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            ++LPRFYHE+V+DH++NPRNVGSFDK DPT+GTGLVGAPACGDVMKLQIKVD+ TG+I+DARFKTFGCGSAIASSSVATEWVKGKQMEEV+TIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY+
Sbjct:   16 QILPRFYHEKVIDHFNNPRNVGSFDKTDPTVGTGLVGAPACGDVMKLQIKVDDTTGEIIDARFKTFGCGSAIASSSVATEWVKGKQMEEVMTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYQ 142

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BLAST of GT621129 vs. TrEMBL
Match: O49627_ARATH (AT4g22220/T10I14_50 OS=Arabidopsis thaliana GN=isu1 PE=2 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 5.873e-61
Identity = 119/144 (82.64%), Postives = 129/144 (89.58%), Query Frame = 2

Query:   29 AKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            AK+ +G  S +    +V +L R YHE V+DHYDNPRNVGSFDKNDP +GTGLVGAPACGDVMKLQIKVDE TG+IVDARFKTFGCGSAIASSSVATEWVKGK ME+VLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY+
Sbjct:    7 AKKALGLTSRQSTPWSVGIL-RTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYK 149

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BLAST of GT621129 vs. TrEMBL
Match: Q8L984_ARATH (NifU-like protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 1.308e-60
Identity = 118/144 (81.94%), Postives = 128/144 (88.89%), Query Frame = 2

Query:   29 AKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            AK+ +G  S +    +V +  R YHE V+DHYDNPRNVGSFDKNDP +GTGLVGAPACGDVMKLQIKVDE TG+IVDARFKTFGCGSAIASSSVATEWVKGK ME+VLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY+
Sbjct:    6 AKKALGLTSRQSTPWSVSIF-RTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYK 148

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BLAST of GT621129 vs. TrEMBL
Match: D7ME63_ARALY (Iron-sulfur cluster assembly complex protein OS=Arabidopsis lyrata subsp. lyrata GN=ISU1 PE=4 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 3.807e-60
Identity = 117/144 (81.25%), Postives = 128/144 (88.89%), Query Frame = 2

Query:   29 AKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            AK+ +G  S +    +V +  R YHE V+DHYDNPRNVGSFDKNDP +GTGLVGAPACGDVMKLQIKVDE TG+IVDARFKTFGCGSAIASSSVATEWVKGK M++VLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY+
Sbjct:    7 AKKALGLTSRQSTPWSVGIF-RSYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMDDVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYK 149

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BLAST of GT621129 vs. TrEMBL
Match: C5YVY2_SORBI (Putative uncharacterized protein Sb09g028890 OS=Sorghum bicolor GN=Sb09g028890 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 3.223e-59
Identity = 120/157 (76.43%), Postives = 134/157 (85.35%), Query Frame = 2

Query:   17 MLRIA-KRLVGTASFEM--PIP------AVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            MLR A +RL+G    E   P+P      AV +  R YHERVVDHY+NPRNVGSFDK+D  +GTG+VGAPACGDVMKLQI+VDE +G+IVDARFKTFGCGSAIASSSVA+EW+KGKQMEEV+ IKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE
Sbjct:    2 MLRAAGRRLLGLGGGEAAPPVPPVATVAAVAIRGRGYHERVVDHYNNPRNVGSFDKDDADVGTGIVGAPACGDVMKLQIRVDEGSGRIVDARFKTFGCGSAIASSSVASEWIKGKQMEEVVAIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 158

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BLAST of GT621129 vs. SwissProt
Match: ISCU_HUMAN (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial OS=Homo sapiens GN=ISCU PE=1 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 9.280e-53
Identity = 105/149 (70.47%), Postives = 121/149 (81.21%), Query Frame = 2

Query:   14 KMLRIAKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            ++ R+A  L+   S  +P   +    R YH++VVDHY+NPRNVGS DK    +GTGLVGAPACGDVMKLQI+VDE  GKIVDARFKTFGCGSAIASSS+ATEWVKGK +EE LTIKNT+IAK L LPPVKLHCSMLAEDAIKAA+ DY+
Sbjct:    8 RLRRVASALL-LRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDE-KGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYK 154

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BLAST of GT621129 vs. SwissProt
Match: ISCU_MOUSE (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial OS=Mus musculus GN=Iscu PE=1 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 1.583e-52
Identity = 105/147 (71.43%), Postives = 118/147 (80.27%), Query Frame = 2

Query:   20 LRIAKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            LR A   +   S  +P   +    R YH++VVDHY+NPRNVGS DK    +GTGLVGAPACGDVMKLQI+VDE  GKIVDARFKTFGCGSAIASSS+ATEWVKGK +EE LTIKNT+IAK L LPPVKLHCSMLAEDAIKAA+ DY+
Sbjct:   10 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDE-KGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYK 155

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BLAST of GT621129 vs. SwissProt
Match: ISU1_KLULA (Iron sulfur cluster assembly protein 1, mitochondrial OS=Kluyveromyces lactis GN=ISU1 PE=3 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 3.301e-50
Identity = 98/147 (66.67%), Postives = 115/147 (78.23%), Query Frame = 2

Query:   35 RLVGTASFEMPIPAVRVLP-----RFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            R++G A+     P V   P     RFYH +V+DHY NPRNVGS DKN P +GTGLVGAPACGDVMKLQI+V++ TG I + +FKTFGCGSAIASSS  TE V+GK +E+   IKNTEIA+ LSLPPVKLHCSMLAEDAIKAA+KDY+
Sbjct:   25 RIIGVAAARATAPTVGSSPSMASARFYHPKVIDHYTNPRNVGSLDKNLPNVGTGLVGAPACGDVMKLQIQVNDETGVIENVKFKTFGCGSAIASSSYMTELVRGKTLEDAAKIKNTEIARELSLPPVKLHCSMLAEDAIKAAIKDYQ 171

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BLAST of GT621129 vs. SwissProt
Match: ISU1_DEBHA (Iron sulfur cluster assembly protein 1, mitochondrial OS=Debaryomyces hansenii GN=ISU1 PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 5.270e-48
Identity = 99/149 (66.44%), Postives = 114/149 (76.51%), Query Frame = 2

Query:   35 RLVGTASFEMP-IPAVRVLP-------RFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY 457
            RL   A   MP +  V ++P       R YHE+V+DHY NPRNVG+ +K D  +GTGLVGAPACGDVM+LQI+VD+ TG I D +FKTFGCGSAIASSS  TE VKGK +EE + IKNT IAK LSLPPVKLHCSMLAEDAIK+AVKDY
Sbjct:    8 RLANPARRAMPAVKRVNMMPSMALPTKRLYHEKVLDHYSNPRNVGTLNKLDVDVGTGLVGAPACGDVMRLQIQVDDETGVIKDVKFKTFGCGSAIASSSYLTELVKGKTIEEAVKIKNTAIAKELSLPPVKLHCSMLAEDAIKSAVKDY 156

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BLAST of GT621129 vs. SwissProt
Match: NIFU_SHIFL (NifU-like protein OS=Shigella flexneri GN=nifU PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.298e-46
Identity = 87/121 (71.90%), Postives = 105/121 (86.78%), Query Frame = 2

Query:   98 YHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            Y E+V+DHY+NPRNVGSFD ND  +G+G+VGAPACGDVMKLQIKV++  G I DARFKT+GCGSAIASSS+ TEWVKGK ++E   IKNT+IA+ L LPPVK+HCS+LAEDAIKAA+ DY+
Sbjct:    3 YSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVND-EGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYK 122

[Close]

BLAST of GT621129 vs. SwissProt
Match: NIFU_ECOLI (NifU-like protein OS=Escherichia coli (strain K12) GN=nifU PE=1 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.298e-46
Identity = 87/121 (71.90%), Postives = 105/121 (86.78%), Query Frame = 2

Query:   98 YHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            Y E+V+DHY+NPRNVGSFD ND  +G+G+VGAPACGDVMKLQIKV++  G I DARFKT+GCGSAIASSS+ TEWVKGK ++E   IKNT+IA+ L LPPVK+HCS+LAEDAIKAA+ DY+
Sbjct:    3 YSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVND-EGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYK 122

[Close]

BLAST of GT621129 vs. SwissProt
Match: NIFU_ECOL6 (NifU-like protein OS=Escherichia coli O6 GN=nifU PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.298e-46
Identity = 87/121 (71.90%), Postives = 105/121 (86.78%), Query Frame = 2

Query:   98 YHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            Y E+V+DHY+NPRNVGSFD ND  +G+G+VGAPACGDVMKLQIKV++  G I DARFKT+GCGSAIASSS+ TEWVKGK ++E   IKNT+IA+ L LPPVK+HCS+LAEDAIKAA+ DY+
Sbjct:    3 YSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVND-EGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYK 122

[Close]

BLAST of GT621129 vs. SwissProt
Match: NIFU_ECO57 (NifU-like protein OS=Escherichia coli O157:H7 GN=nifU PE=1 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.298e-46
Identity = 87/121 (71.90%), Postives = 105/121 (86.78%), Query Frame = 2

Query:   98 YHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            Y E+V+DHY+NPRNVGSFD ND  +G+G+VGAPACGDVMKLQIKV++  G I DARFKT+GCGSAIASSS+ TEWVKGK ++E   IKNT+IA+ L LPPVK+HCS+LAEDAIKAA+ DY+
Sbjct:    3 YSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVND-EGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYK 122

[Close]

BLAST of GT621129 vs. SwissProt
Match: ISU1_YARLI (Iron sulfur cluster assembly protein 1, mitochondrial OS=Yarrowia lipolytica GN=ISU1 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.695e-46
Identity = 94/152 (61.84%), Postives = 114/152 (75.00%), Query Frame = 2

Query:    2 KLKRKMLRIAKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY 457
            ++ R    +A+    T +   P+  + +  R YHE+V+DHY+NPRNVGS +KND  +GTGLVGAPACGDVMKLQI+VD+  G I D +FKTFGCGSAIASSS  TE V+GK + E   IKNT IAK LSLPPVKLHCSMLAEDAIK+A+ DY
Sbjct:    5 RITRSAGLLARARPTTTAVMSPMTPLAISRRGYHEKVLDHYNNPRNVGSMNKNDEDVGTGLVGAPACGDVMKLQIRVDD-NGVIQDVKFKTFGCGSAIASSSYVTELVRGKSLAEAGKIKNTVIAKELSLPPVKLHCSMLAEDAIKSAISDY 155

[Close]

BLAST of GT621129 vs. SwissProt
Match: ISU1_YEAST (Iron sulfur cluster assembly protein 1, mitochondrial OS=Saccharomyces cerevisiae GN=ISU1 PE=1 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 2.214e-46
Identity = 89/129 (68.99%), Postives = 105/129 (81.40%), Query Frame = 2

Query:   74 AVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            A  +  R YH +V++HY +PRNVGS DK  P +GTGLVGAPACGDVM+LQIKV++ TG I D +FKTFGCGSAIASSS  TE V+G  +++   IKNTEIAK LSLPPVKLHCSMLAEDAIKAA+KDY+
Sbjct:   27 ASSITKRLYHPKVIEHYTHPRNVGSLDKKLPNVGTGLVGAPACGDVMRLQIKVNDSTGVIEDVKFKTFGCGSAIASSSYMTELVQGMTLDDAAKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYK 155

[Close]

BLAST of GT621129 vs. TAIR peptide
Match: AT4G22220.1 (| Symbols: ISU1, ATISU1 | SufE/NifU family protein | chr4:11759444-11760881 REVERSE LENGTH=167)

HSP 1 Score: 238.424 bits (607), Expect = 3.705e-63
Identity = 119/144 (82.64%), Postives = 129/144 (89.58%), Query Frame = 2

Query:   29 AKRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            AK+ +G  S +    +V +L R YHE V+DHYDNPRNVGSFDKNDP +GTGLVGAPACGDVMKLQIKVDE TG+IVDARFKTFGCGSAIASSSVATEWVKGK ME+VLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY+
Sbjct:    7 AKKALGLTSRQSTPWSVGIL-RTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYK 149

[Close]

BLAST of GT621129 vs. TAIR peptide
Match: AT4G04080.1 (| Symbols: ISU3, ATISU3 | ISCU-like 3 | chr4:1963386-1964308 FORWARD LENGTH=171)

HSP 1 Score: 215.698 bits (548), Expect = 2.571e-56
Identity = 106/143 (74.13%), Postives = 124/143 (86.71%), Query Frame = 2

Query:   32 KRLVGTASFEMPIPAVRVLPRFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            +  +G AS + P P   V+ R YH  V+DHYDNPRNVGSFDKNDP +GTGLVGAP CGDVMKLQ+K D  +G+I+DA+FKTFGCGSAIA+SSVATEWVKGK +EEVLTIKN++IAKHLSLPPVKLHCSMLAEDAIKAA+K+Y+
Sbjct:    8 RAFLGLAS-QNPTP-FPVVSRLYHPNVIDHYDNPRNVGSFDKNDPNVGTGLVGAPQCGDVMKLQVKFDG-SGQIIDAKFKTFGCGSAIAASSVATEWVKGKSVEEVLTIKNSQIAKHLSLPPVKLHCSMLAEDAIKAAIKNYK 147

[Close]

BLAST of GT621129 vs. TAIR peptide
Match: AT3G01020.1 (| Symbols: ISU2, ATISU2 | ISCU-like 2 | chr3:5139-5893 FORWARD LENGTH=163)

HSP 1 Score: 208.379 bits (529), Expect = 4.105e-54
Identity = 95/123 (77.24%), Postives = 114/123 (92.68%), Query Frame = 2

Query:   92 RFYHERVVDHYDNPRNVGSFDKNDPTIGTGLVGAPACGDVMKLQIKVDEVTGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYE 460
            R YHE V+DH++NPRNVGSF++NDP +GTGLVGAPACGD+M LQIKVD+ +G+I+D RFKTFGCGSAIASSSVA+EW+KGK ++EV+TIKN EIAKHL LPPVKLHCSMLAEDAIK+AV+DY+
Sbjct:   25 RLYHENVIDHFENPRNVGSFNRNDPNVGTGLVGAPACGDLMSLQIKVDD-SGQIIDTRFKTFGCGSAIASSSVASEWIKGKTLDEVMTIKNAEIAKHLRLPPVKLHCSMLAEDAIKSAVRDYK 146

The following BLAST results are available for this feature:

BLAST of GT621129 vs. TrEMBL
BLAST of GT621129 vs. SwissProt
BLAST of GT621129 vs. TAIR peptide

BLAST of GT621129 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FHC6_MEDTR	1.760e-73	93.92	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6T2J7_SOYBN	5.133e-65	85.23	Putative uncharacterized protein OS=Glycine max PE...	[more]
B8R1J5_EUCGR	2.157e-63	86.09	Iron-sulfer cluster scaffold protein ISU1 OS=Eucal...	[more]
B9RB15_RICCO	3.679e-63	83.89	Nitrogen fixation protein nifu, putative OS=Ricinu...	[more]
A9PEA4_POPTR	4.805e-63	89.76	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PDY0_POPTR	1.826e-62	88.98	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
O49627_ARATH	5.873e-61	82.64	AT4g22220/T10I14_50 OS=Arabidopsis thaliana GN=isu...	[more]
Q8L984_ARATH	1.308e-60	81.94	NifU-like protein OS=Arabidopsis thaliana PE=2 SV=...	[more]
D7ME63_ARALY	3.807e-60	81.25	Iron-sulfur cluster assembly complex protein OS=Ar...	[more]
C5YVY2_SORBI	3.223e-59	76.43	Putative uncharacterized protein Sb09g028890 OS=So...	[more]

BLAST of GT621129 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ISCU_HUMAN	9.280e-53	70.47	Iron-sulfur cluster assembly enzyme ISCU, mitochon...	[more]
ISCU_MOUSE	1.583e-52	71.43	Iron-sulfur cluster assembly enzyme ISCU, mitochon...	[more]
ISU1_KLULA	3.301e-50	66.67	Iron sulfur cluster assembly protein 1, mitochondr...	[more]
ISU1_DEBHA	5.270e-48	66.44	Iron sulfur cluster assembly protein 1, mitochondr...	[more]
NIFU_SHIFL	1.298e-46	71.90	NifU-like protein OS=Shigella flexneri GN=nifU PE=...	[more]
NIFU_ECOLI	1.298e-46	71.90	NifU-like protein OS=Escherichia coli (strain K12)...	[more]
NIFU_ECOL6	1.298e-46	71.90	NifU-like protein OS=Escherichia coli O6 GN=nifU P...	[more]
NIFU_ECO57	1.298e-46	71.90	NifU-like protein OS=Escherichia coli O157:H7 GN=n...	[more]
ISU1_YARLI	1.695e-46	61.84	Iron sulfur cluster assembly protein 1, mitochondr...	[more]
ISU1_YEAST	2.214e-46	68.99	Iron sulfur cluster assembly protein 1, mitochondr...	[more]

BLAST of GT621129 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G22220.1	3.705e-63	82.64	\| Symbols: ISU1, ATISU1 \| SufE/NifU family protein...	[more]
AT4G04080.1	2.571e-56	74.13	\| Symbols: ISU3, ATISU3 \| ISCU-like 3 \| chr4:19633...	[more]
AT3G01020.1	4.105e-54	77.24	\| Symbols: ISU2, ATISU2 \| ISCU-like 2 \| chr3:5139-...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002871	NIF system FeS cluster assembly, NifU, N-terminal	PANTHER	PTHR10093	IRON-SULFUR CLUSTER ASSEMBLY ENZYME (NIFU HOMOLOG)	coord: 31..155 score: 5
IPR002871	NIF system FeS cluster assembly, NifU, N-terminal	PFAM	PF01592	NifU_N	coord: 33..157 score: 7.2
IPR011339	ISC system FeS cluster assembly, IscU scaffold	TIGRFAMs	TIGR01999	iscU: FeS cluster assembly scaffold IscU	coord: 33..157 score: 2.7
None	No IPR available	GENE3D	3.90.1010.10	no description	coord: 32..157 score: 1.9
None	No IPR available	SEG	seg	seg	coord: 154..163 score
None	No IPR available	SUPERFAMILY	82649	SufE/NifU	coord: 31..156 score: 8.4
None	No IPR available	SUPERFAMILY	57059	omega toxin-like	coord: 38..74 score: 1.1
None	No IPR available	SEG	seg	seg	coord: 13..38 score
IPR012512	Albumin I	PFAM	PF08027	Albumin_I	coord: 18..75 score: 2.9

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT621129 ID=GT621129; Name=GT621129; organism=Lens culinaris; type=EST; length=840bp
AAAATTGAAAAGAAAAATGCTTAGAATCGCAAAGAGACTCGTGGGAACGG
CGTCGTTTGAAATGCCAATTCCCGCCGTTAGGGTTCTTCCACGATTCTAC
CACGAGAGGGTGGTGGATCATTACGATAATCCCCGTAATGTTGGATCCTT
TGACAAGAACGACCCGACGATAGGTACCGGTCTTGTTGGAGCACCCGCGT
GCGGTGATGTCATGAAGCTCCAAATTAAGGTTGATGAGGTCACCGGAAAA
ATCGTCGATGCTCGCTTTAAAACCTTTGGATGCGGATCCGCCATTGCTTC
CTCATCCGTCGCTACTGAATGGGTTAAAGGAAAGCAAATGGAAGAGGTTT
TGACCATTAAGAACACTGAGATTGCAAAGCATCTTTCACTCCCACCGGTT
AAGCTTCACTGCAGCATGCTTGCTGAGGATGCTATAAAAGCAGCTGTTAA
AGATTATGAAGCTAAAAAAGCCTCTGCTGCTGCCACTGCGTGATTTCTTC
CATTTCTTGAATGATGATGGAAAAAAAAGCATTAGTTTGATCGATTGCAC
ATCATTAAAACATTAGTCAGACTTTTCTTGGTTGGAAGTACACTGACATA
TTGTATCTCATAAAACAACTGTAGCGGGTTTTCTTTTGATCGTACAATGG
CTTATGTTAAACTCGCTTCTTTGATTGTCTTGTTTGCCACATTCGGTATA
TTCCAGACAAAGAACGCAGGAGCAGCAGACTGTAATGGGGCTTGTTCTCC
ATTTGAGATGCCACCGTGTCGCTCTTCAGCCTGTCGCTGTATCCCTGTTG
GTCTAGTTGTAGGTTATTGCAGACATCCATCTTCATTGAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0051536	iron-sulfur cluster binding
GO:0005515	protein binding
GO:0045735	nutrient reservoir activity

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly
GO:0009405	obsolete pathogenesis

Vocabulary: **INTERPRO**
Term	Definition
IPR002871	NIF_FeS_clus_asmbl_NifU_N
IPR011339	ISCU
IPR012512	Albumin_I

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