GT622199

EST Overview


Name	GT622199
Unique Name	GT622199
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	743

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT622199 vs. TrEMBL
Match: B9RKH3_RICCO (Mitochondrial import inner membrane translocase subunit tim17, putative OS=Ricinus communis GN=RCOM_1049400 PE=4 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 4.710e-53
Identity = 107/165 (64.85%), Postives = 124/165 (75.15%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDE---PVSGHPNXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            AVR+NAPRVGGSFAVWGGLFS+FDCTMVY RQKEDPWNSIFAGAATGGFLSMRQGL AS R+A FGG+LL LIEGAGIMLNKV+S QQ +P ++D+    ++G P+                  WFGG   G   K +EP++  G +T+ILESFDAPPVPNFEYK
Sbjct:   54 AVRMNAPRVGGSFAVWGGLFSAFDCTMVYARQKEDPWNSIFAGAATGGFLSMRQGLGASARAALFGGILLGLIEGAGIMLNKVMSAQQNMPVMIDDSAPAMAGLPSLPGQEPASTSSGSESGSSWFGGWFGG--GKTEEPITSKGGKTEILESFDAPPVPNFEYK 216

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BLAST of GT622199 vs. TrEMBL
Match: B9N332_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_750203 PE=4 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 6.152e-53
Identity = 114/170 (67.06%), Postives = 129/170 (75.88%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEPV----SGHPNXXXXXXXXXXXXXS----EAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            AVR+NAPRVGGSFAVWGGLFS+FDC+MVYLRQKEDPWNSI AGAATGGFLSMRQGL ASGRSA FGGVLLALIEGAGIMLNKV+S QQ +P ++++PV     GH +             S     +  WFGG  FG  KK+ E  +  GS+T+ILESFDAPPVPNFEYK
Sbjct:   54 AVRMNAPRVGGSFAVWGGLFSAFDCSMVYLRQKEDPWNSIIAGAATGGFLSMRQGLGASGRSALFGGVLLALIEGAGIMLNKVMSAQQEMPIMIEDPVPSMAGGHGSPMGQPHAHAQEGASASGTNSGSWFGG-WFGGGKKESE-ANISGSKTEILESFDAPPVPNFEYK 221

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BLAST of GT622199 vs. TrEMBL
Match: B9HKZ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_565047 PE=4 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 3.053e-52
Identity = 107/163 (65.64%), Postives = 121/163 (74.23%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDE-PVSGHPNXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            AVR+NAPRVGGSFAVWGGLFS+FDC+MVYLRQKEDPWNSI AGAATGGFLSMRQGL AS RSA FGGVLL LIEGAGIMLNKV+  QQ +P ++DE P+                   E+  WFGG   GE KKK+   +   S+T++LESFDAPPVP FEYK
Sbjct:   54 AVRMNAPRVGGSFAVWGGLFSAFDCSMVYLRQKEDPWNSIIAGAATGGFLSMRQGLGASARSAVFGGVLLGLIEGAGIMLNKVMGDQQSMPIMIDEDPIPAMAGGGPGGFPMGQAQEGESGSWFGGWFGGEKKKKES--TAASSKTEVLESFDAPPVPTFEYK 214

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BLAST of GT622199 vs. TrEMBL
Match: D7TBG4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_16.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00015301001 PE=4 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 1.160e-51
Identity = 111/163 (68.10%), Postives = 123/163 (75.46%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEPVSGHPNXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAP-PVPNFEYK 486
            AVR+NAPRVGGSFAVWGGLFS+FDCTMVY+RQKEDPWNSI AGAATGGFL MRQGL AS RSA FGGVLL LIEGAGIMLNKVLS  Q +P  M+EPV G                S +  WFGG LFG  KK+ EP +  GS+T+ILESFD P P+P+FEYK
Sbjct:   54 AVRMNAPRVGGSFAVWGGLFSAFDCTMVYVRQKEDPWNSIIAGAATGGFLQMRQGLAASTRSAIFGGVLLGLIEGAGIMLNKVLSAPQNLPMTMEEPVPG---------MTPGPPSSGSSSWFGG-LFGGGKKQ-EPAANSGSKTEILESFDTPTPMPSFEYK 205

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BLAST of GT622199 vs. TrEMBL
Match: Q38M66_SOLTU (Putative uncharacterized protein OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 9.193e-49
Identity = 112/186 (60.22%), Postives = 124/186 (66.67%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEP------VSGHP------------------NXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            AVR+NAPR+GGSFAVWGGLFS+FDCTMVYLRQKEDPWNSI AGAATGGFL MRQGL A+ RSA FGGVLLALIEGAGIMLNKV+S  Q  P  MDEP      V G+P                  +             S +  WFGG LFG  KK +E  S GGS+T++LESFDAP  P FEYK
Sbjct:   54 AVRMNAPRIGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLQMRQGLGAASRSAMFGGVLLALIEGAGIMLNKVMSAPQNFPP-MDEPLPNVPGVPGYPPGQLPGQPMGKLPGQAPVSIDGMMTESSAPSSSSSTSWFGG-LFGGGKK-EETTSSGGSKTQVLESFDAPSPPTFEYK 236

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BLAST of GT622199 vs. TrEMBL
Match: C5XDW4_SORBI (Putative uncharacterized protein Sb02g038750 OS=Sorghum bicolor GN=Sb02g038750 PE=4 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.917e-46
Identity = 103/172 (59.88%), Postives = 124/172 (72.09%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVP--------AIMDEPVSGHPNXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAP--PVPNFEYK 486
            AVR+NAPRVGGSFAVWGGLFS+FDC MVY RQKEDPWNSI AGAATGGFLSMRQG+ A+GRSA  GG+LLALIEGAG+MLN+VL+   P+P        A+   P  G P              S A  WFGG LFG+ +++ +P +GGG +++ILESFD P  P+P+FEYK
Sbjct:   54 AVRMNAPRVGGSFAVWGGLFSTFDCAMVYARQKEDPWNSIVAGAATGGFLSMRQGMGAAGRSALMGGILLALIEGAGLMLNRVLA-NPPLPADDPNLTAAMGGNPFPGLPQAPPVVAPPEAASSSGAGGWFGG-LFGKKEEEKKPSAGGG-KSEILESFDTPSSPIPSFEYK 222

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BLAST of GT622199 vs. TrEMBL
Match: A1YMY1_BRACM (Protein translocase OS=Brassica campestris PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 3.270e-46
Identity = 103/171 (60.23%), Postives = 122/171 (71.35%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEPVSGHPNXXXXXXXXXXXXXSEAKP---------WFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            AV +NAPR+GGSFAVWGGLFS+FDC+MVYLRQKEDPWNSI AGAATGGFLSMRQG  A+ RSA  GGVLLALIEGAGI LNK+++  +P    M+E ++G P               +  P         WFGG LFG+  ++ +P S  GSETK+LESFDAPPVP+FEYK
Sbjct:   54 AVAMNAPRLGGSFAVWGGLFSTFDCSMVYLRQKEDPWNSIIAGAATGGFLSMRQGPNAAVRSALVGGVLLALIEGAGIALNKMMA--EPQHMQMEEGMAGVPGIQMGQQQQQVPNQGQMIPPPENTASSSWFGG-LFGKKNEETQPTS-SGSETKVLESFDAPPVPSFEYK 220

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BLAST of GT622199 vs. TrEMBL
Match: B6SUP6_MAIZE (Mitochondrial import inner membrane translocase subunit Tim17 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 3.615e-45
Identity = 101/172 (58.72%), Postives = 117/172 (68.02%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVP--------AIMDEPVSGHPNXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAP--PVPNFEYK 486
            AVR+NAPRVGGSFAVWGGLFS+FDC MVY RQKEDPWNSI AGAATGGFLSMRQG+ A+GRSA  GGVLLALIEGAG+MLN+VL+   P+P        A+ D P  G P              S A  WFGG +     +KDE       +++ILESFD P  P+P+FEYK
Sbjct:   54 AVRMNAPRVGGSFAVWGGLFSTFDCAMVYARQKEDPWNSIIAGAATGGFLSMRQGMGAAGRSALMGGVLLALIEGAGLMLNRVLA-NPPLPAEDPNLTAAMGDNPFPGLPQAPPVVAPPEVASSSGAGGWFGGLI----GRKDEEKKSSAGKSEILESFDTPSSPIPSFEYK 220

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BLAST of GT622199 vs. TrEMBL
Match: Q6S710_ARATH (Mitochondrial inner membrane translocase TM17-2 (Fragment) OS=Arabidopsis thaliana GN=At2g37410 PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 2.343e-44
Identity = 104/195 (53.33%), Postives = 121/195 (62.05%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEP-VSGHP--------------------------------NXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            +V +NAPR GGSFAVWGGLFS+FDCTMVYLRQKEDPWNSI AGAATGGFLSMRQG  A+ RSA FGGVLLALIEGAGIMLN+VL+  QP   +M++P + G P                                              S +  WFGG     DKKK+E   G  S+T++LESFDAPPVP+FE+K
Sbjct:   54 SVSMNAPRTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMRQGAGAASRSAIFGGVLLALIEGAGIMLNEVLA--QPQNMMMEDPGMQGMPGMQGMQGMPGMPGMQGMPGMQGMQMGQMQSQAQIRSESQNQNTASSSSSSSWFGGLF---DKKKEEVQPGSESKTEVLESFDAPPVPSFEFK 243

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BLAST of GT622199 vs. TrEMBL
Match: C0HHS3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 6.817e-44
Identity = 100/171 (58.48%), Postives = 120/171 (70.18%), Query Frame = 1

Query:    4 VRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVP--------AIMDEPVSGHPNXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAP--PVPNFEYK 486
            VR+NAPRVGGSFAVWGGLFS+FDC MVY RQKEDPWNSI AGAATGGFLSMRQG+ A+GRSA  GG+LLALIEGAG+MLN+VL+   P+P        A+   P  G P              S     FGG LFG  +++ +P +GGG +++ILESFD P  P+P+FEYK
Sbjct:   55 VRMNAPRVGGSFAVWGGLFSTFDCAMVYARQKEDPWNSIVAGAATGGFLSMRQGMGAAGRSALMGGILLALIEGAGLMLNRVLA-NPPLPADDPNLTAAMGGNPFPGLPQAPPVVAPPEATTSSGVGGLFGG-LFGRKEEEKKPSAGGG-KSEILESFDTPSSPIPSFEYK 222

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BLAST of GT622199 vs. SwissProt
Match: TIM17_ARATH (Mitochondrial import inner membrane translocase subunit Tim17 OS=Arabidopsis thaliana GN=TIM17 PE=2 SV=2)

HSP 1 Score: 184.496 bits (467), Expect = 5.300e-46
Identity = 105/195 (53.85%), Postives = 121/195 (62.05%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEP-VSGHP--------------------------------NXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            +V +NAPR GGSFAVWGGLFS+FDCTMVYLRQKEDPWNSI AGAATGGFLSMRQG  A+ RSA FGGVLLALIEGAGIMLNKVL+  QP   +M++P + G P                                              S +  WFGG     DKKK+E   G  S+T++LESFDAPPVP+FE+K
Sbjct:   54 SVSMNAPRTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMRQGAGAASRSAIFGGVLLALIEGAGIMLNKVLA--QPQNMMMEDPGMQGMPGMQGMQGMPGMPGMQGMPGMQGMQMGQMQSQAQIRSESQNQNTASSSSSSSWFGGLF---DKKKEEVQPGSESKTEVLESFDAPPVPSFEFK 243

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BLAST of GT622199 vs. SwissProt
Match: TI17A_MOUSE (Mitochondrial import inner membrane translocase subunit Tim17-A OS=Mus musculus GN=Timm17a PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.773e-25
Identity = 56/89 (62.92%), Postives = 68/89 (76.40%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQP 267
            A++  AP++GGSFAVWGGLFS+ DC+MV +R KEDPWNSI +GA TG  L+ R G VA   SAA GG+LLALIEGAGI+L +  S Q P
Sbjct:   54 AIKTRAPQLGGSFAVWGGLFSTIDCSMVQIRGKEDPWNSITSGALTGAILAARNGPVAMVGSAAMGGILLALIEGAGILLTRFASAQFP 142

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BLAST of GT622199 vs. SwissProt
Match: TI17A_HUMAN (Mitochondrial import inner membrane translocase subunit Tim17-A OS=Homo sapiens GN=TIMM17A PE=1 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 3.024e-25
Identity = 58/98 (59.18%), Postives = 71/98 (72.45%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQP-VPAIMDEP 291
            A++  AP++GGSFAVWGGLFS  DC+MV +R KEDPWNSI +GA TG  L+ R G VA   SAA GG+LLALIEGAGI+L +  S Q P  P   ++P
Sbjct:   54 AIKTRAPQLGGSFAVWGGLFSMIDCSMVQVRGKEDPWNSITSGALTGAILAARNGPVAMVGSAAMGGILLALIEGAGILLTRFASAQFPNGPQFAEDP 151

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BLAST of GT622199 vs. SwissProt
Match: TI17A_RAT (Mitochondrial import inner membrane translocase subunit Tim17-A OS=Rattus norvegicus GN=Timm17a PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 3.949e-25
Identity = 56/89 (62.92%), Postives = 67/89 (75.28%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQP 267
            A++  AP++GGSFAVWGGLFS+ DC MV +R KEDPWNSI +GA TG  L+ R G VA   SAA GG+LLALIEGAGI+L +  S Q P
Sbjct:   54 AIKTRAPQLGGSFAVWGGLFSTIDCGMVQIRGKEDPWNSITSGALTGAILAARNGPVAMVGSAAMGGILLALIEGAGILLTRFASAQFP 142

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BLAST of GT622199 vs. SwissProt
Match: TI17B_MOUSE (Mitochondrial import inner membrane translocase subunit Tim17-B OS=Mus musculus GN=Timm17b PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 4.366e-24
Identity = 55/98 (56.12%), Postives = 71/98 (72.45%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQ-QPVPAIMDEP 291
            AVR+ AP++GGSFAVWGGLFS+ DC +V LR KEDPWNSI +GA TG  L+ R G +A   SA  GG+LLALIEG GI+L +  + Q +  P  +++P
Sbjct:   54 AVRIRAPQIGGSFAVWGGLFSTIDCGLVRLRGKEDPWNSISSGALTGAVLAARSGPLAMVGSAMMGGILLALIEGVGILLTRYTAQQFRNAPPFLEDP 151

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BLAST of GT622199 vs. SwissProt
Match: TI17B_HUMAN (Mitochondrial import inner membrane translocase subunit Tim17-B OS=Homo sapiens GN=TIMM17B PE=1 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 4.366e-24
Identity = 55/98 (56.12%), Postives = 71/98 (72.45%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQ-QPVPAIMDEP 291
            AVR+ AP++GGSFAVWGGLFS+ DC +V LR KEDPWNSI +GA TG  L+ R G +A   SA  GG+LLALIEG GI+L +  + Q +  P  +++P
Sbjct:   54 AVRIRAPQIGGSFAVWGGLFSTIDCGLVRLRGKEDPWNSITSGALTGAVLAARSGPLAMVGSAMMGGILLALIEGVGILLTRYTAQQFRNAPPFLEDP 151

[Close]

BLAST of GT622199 vs. SwissProt
Match: TI17B_BOVIN (Mitochondrial import inner membrane translocase subunit Tim17-B OS=Bos taurus GN=TIMM17B PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 4.827e-23
Identity = 54/98 (55.10%), Postives = 70/98 (71.43%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQ-QPVPAIMDEP 291
            AVR+ AP++GGSFAVWGGLFS+ DC +V LR KEDPWNSI +GA TG  L+ R   +A   SA  GG+LLALIEG GI+L +  + Q +  P  +++P
Sbjct:   54 AVRIRAPQIGGSFAVWGGLFSTIDCGLVRLRGKEDPWNSITSGALTGAVLAARSVPLAMVGSAMMGGILLALIEGVGILLTRYTAQQFRNAPPFLEDP 151

[Close]

BLAST of GT622199 vs. SwissProt
Match: TI17A_DROME (Probable mitochondrial import inner membrane translocase subunit Tim17 1 OS=Drosophila melanogaster GN=Tim17b1 PE=2 SV=2)

HSP 1 Score: 100.908 bits (250), Expect = 7.707e-21
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLN 243
            AVR  +  VGG+FAVWG  FS+ DC++VY R+KEDPWN+I +GA TGG L+ R GL +   SA  GG LLALIEG GI+++
Sbjct:   54 AVRARSGLVGGNFAVWGATFSAIDCSLVYFRKKEDPWNAIISGATTGGILAARTGLTSMLSSALVGGALLALIEGVGIVVS 134

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BLAST of GT622199 vs. SwissProt
Match: TIM17_NEUCR (Mitochondrial import inner membrane translocase subunit tim-17 OS=Neurospora crassa GN=tim-17 PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.898e-19
Identity = 52/103 (50.49%), Postives = 67/103 (65.05%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQ-KEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSG----QQPVPAIMDEPV 294
            A+++ AP +GG+F VWGGLFS+FDC +  LR  KEDPWNSI AG  TGG L++R G  A+   A    VLLA+IEG GI   K+L+G    + P P   +E V
Sbjct:   51 AIKMRAPALGGNFGVWGGLFSTFDCAIKGLRNHKEDPWNSILAGFFTGGALAVRGGYKAARNGAIGCAVLLAVIEGVGIGFQKMLAGATKLEAPAPPPSNEKV 153

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BLAST of GT622199 vs. SwissProt
Match: TI17C_DROME (Probable mitochondrial import inner membrane translocase subunit Tim17 3 OS=Drosophila melanogaster GN=Tim17a1 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.230e-18
Identity = 42/76 (55.26%), Postives = 55/76 (72.37%), Query Frame = 1

Query:    4 VRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAG 231
            V++  P + GSFAVWG  FS+ DC +V+ RQ+ED WNSI +GAATGG L+ R G+ A   SA  G ++LA+IEGAG
Sbjct:   54 VKMRTPSIAGSFAVWGATFSTVDCALVHYRQREDAWNSILSGAATGGILAARNGIRAMANSALVGCLVLAMIEGAG 129

[Close]

BLAST of GT622199 vs. TAIR peptide
Match: AT2G37410.2 (| Symbols: ATTIM17-2, TIM17, TIM17-2 | translocase inner membrane subunit 17-2 | chr2:15698119-15698850 REVERSE LENGTH=243)

HSP 1 Score: 184.496 bits (467), Expect = 5.272e-47
Identity = 105/195 (53.85%), Postives = 121/195 (62.05%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEP-VSGHP--------------------------------NXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            +V +NAPR GGSFAVWGGLFS+FDCTMVYLRQKEDPWNSI AGAATGGFLSMRQG  A+ RSA FGGVLLALIEGAGIMLNKVL+  QP   +M++P + G P                                              S +  WFGG     DKKK+E   G  S+T++LESFDAPPVP+FE+K
Sbjct:   54 SVSMNAPRTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMRQGAGAASRSAIFGGVLLALIEGAGIMLNKVLA--QPQNMMMEDPGMQGMPGMQGMQGMPGMPGMQGMPGMQGMQMGQMQSQAQIRSESQNQNTASSSSSSSWFGGLF---DKKKEEVQPGSESKTEVLESFDAPPVPSFEFK 243

[Close]

BLAST of GT622199 vs. TAIR peptide
Match: AT2G37410.1 (| Symbols: ATTIM17-2, TIM17, TIM17-2 | translocase inner membrane subunit 17-2 | chr2:15698119-15698850 REVERSE LENGTH=243)

HSP 1 Score: 184.496 bits (467), Expect = 5.272e-47
Identity = 105/195 (53.85%), Postives = 121/195 (62.05%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEP-VSGHP--------------------------------NXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            +V +NAPR GGSFAVWGGLFS+FDCTMVYLRQKEDPWNSI AGAATGGFLSMRQG  A+ RSA FGGVLLALIEGAGIMLNKVL+  QP   +M++P + G P                                              S +  WFGG     DKKK+E   G  S+T++LESFDAPPVP+FE+K
Sbjct:   54 SVSMNAPRTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMRQGAGAASRSAIFGGVLLALIEGAGIMLNKVLA--QPQNMMMEDPGMQGMPGMQGMQGMPGMPGMQGMPGMQGMQMGQMQSQAQIRSESQNQNTASSSSSSSWFGGLF---DKKKEEVQPGSESKTEVLESFDAPPVPSFEFK 243

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BLAST of GT622199 vs. TAIR peptide
Match: AT1G20350.1 (| Symbols: ATTIM17-1, TIM17-1 | translocase inner membrane subunit 17-1 | chr1:7043737-7044393 FORWARD LENGTH=218)

HSP 1 Score: 161.77 bits (408), Expect = 3.659e-40
Identity = 90/168 (53.57%), Postives = 108/168 (64.29%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALIEGAGIMLNKVLSGQQPVPAIMDEPVSGHP------NXXXXXXXXXXXXXSEAKPWFGGALFGEDKKKDEPVSGGGSETKILESFDAPPVPNFEYK 486
            A+R++ PR GGSF+VWGGL+S+FDC +VY RQKEDPWNSI +GAATGGFLS+RQGL AS RSA  GGVLLA+IEG GIMLNKV S       + D   +  P      +             S +  WF G+LF   KKK+       S T ILESFDAPPVP +E+K
Sbjct:   54 ALRMSGPRSGGSFSVWGGLYSTFDCALVYARQKEDPWNSILSGAATGGFLSLRQGLGASARSALVGGVLLAMIEGVGIMLNKVQSTAHNEQFMEDHAATSLPYGMGQISGQSVPVPETSSSSSGSVSWF-GSLF--KKKKETEDHHSESRTHILESFDAPPVPTYEFK 218

[Close]

BLAST of GT622199 vs. TAIR peptide
Match: AT5G11690.1 (| Symbols: ATTIM17-3, TIM17-3 | translocase inner membrane subunit 17-3 | chr5:3761759-3762160 FORWARD LENGTH=133)

HSP 1 Score: 93.5893 bits (231), Expect = 1.224e-19
Identity = 44/73 (60.27%), Postives = 58/73 (79.45%), Query Frame = 1

Query:    1 AVRLNAPRVGGSFAVWGGLFSSFDCTMVYLRQKEDPWNSIFAGAATGGFLSMRQGLVASGRSAAFGGVLLALI 219
            A  +NAPR+GG+FAV+GGL S+FD  +V +R+KEDPWNSI AGAATGG LS+R+G+VA+  SA   G  LA++
Sbjct:   54 AASMNAPRLGGTFAVFGGLLSTFDYALVRIRKKEDPWNSIVAGAATGGVLSIRKGVVAASTSAVMFGFFLAVL 126

The following BLAST results are available for this feature:

BLAST of GT622199 vs. TrEMBL
BLAST of GT622199 vs. SwissProt
BLAST of GT622199 vs. TAIR peptide

BLAST of GT622199 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RKH3_RICCO	4.710e-53	64.85	Mitochondrial import inner membrane translocase su...	[more]
B9N332_POPTR	6.152e-53	67.06	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HKZ5_POPTR	3.053e-52	65.64	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TBG4_VITVI	1.160e-51	68.10	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q38M66_SOLTU	9.193e-49	60.22	Putative uncharacterized protein OS=Solanum tubero...	[more]
C5XDW4_SORBI	1.917e-46	59.88	Putative uncharacterized protein Sb02g038750 OS=So...	[more]
A1YMY1_BRACM	3.270e-46	60.23	Protein translocase OS=Brassica campestris PE=2 SV...	[more]
B6SUP6_MAIZE	3.615e-45	58.72	Mitochondrial import inner membrane translocase su...	[more]
Q6S710_ARATH	2.343e-44	53.33	Mitochondrial inner membrane translocase TM17-2 (F...	[more]
C0HHS3_MAIZE	6.817e-44	58.48	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of GT622199 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TIM17_ARATH	5.300e-46	53.85	Mitochondrial import inner membrane translocase su...	[more]
TI17A_MOUSE	1.773e-25	62.92	Mitochondrial import inner membrane translocase su...	[more]
TI17A_HUMAN	3.024e-25	59.18	Mitochondrial import inner membrane translocase su...	[more]
TI17A_RAT	3.949e-25	62.92	Mitochondrial import inner membrane translocase su...	[more]
TI17B_MOUSE	4.366e-24	56.12	Mitochondrial import inner membrane translocase su...	[more]
TI17B_HUMAN	4.366e-24	56.12	Mitochondrial import inner membrane translocase su...	[more]
TI17B_BOVIN	4.827e-23	55.10	Mitochondrial import inner membrane translocase su...	[more]
TI17A_DROME	7.707e-21	58.02	Probable mitochondrial import inner membrane trans...	[more]
TIM17_NEUCR	1.898e-19	50.49	Mitochondrial import inner membrane translocase su...	[more]
TI17C_DROME	1.230e-18	55.26	Probable mitochondrial import inner membrane trans...	[more]

BLAST of GT622199 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT2G37410.2	5.272e-47	53.85	\| Symbols: ATTIM17-2, TIM17, TIM17-2 \| translocase...	[more]
AT2G37410.1	5.272e-47	53.85	\| Symbols: ATTIM17-2, TIM17, TIM17-2 \| translocase...	[more]
AT1G20350.1	3.659e-40	53.57	\| Symbols: ATTIM17-1, TIM17-1 \| translocase inner ...	[more]
AT5G11690.1	1.224e-19	60.27	\| Symbols: ATTIM17-3, TIM17-3 \| translocase inner ...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003397	Mitochondrial inner membrane translocase complex, subunit Tim17/22	PFAM	PF02466	Tim17	coord: 3..79 score: 2.5
None	No IPR available	PANTHER	PTHR10485	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM17	coord: 1..87 score: 4.9
None	No IPR available	SEG	seg	seg	coord: 104..116 score
None	No IPR available	SEG	seg	seg	coord: 56..72 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT622199 ID=GT622199; Name=GT622199; organism=Lens culinaris; type=EST; length=743bp
GCCGTTCGTCTCAACGCTCCCCGTGTCGGCGGTAGTTTCGCCGTTTGGGG
TGGTCTCTTCTCCTCTTTCGACTGCACCATGGTCTACCTCCGCCAAAAAG
AAGACCCTTGGAACTCTATCTTCGCCGGCGCTGCCACCGGTGGTTTTCTC
TCCATGCGTCAAGGTTTAGTCGCTTCCGGGCGGTCTGCTGCGTTCGGTGG
TGTTCTCTTGGCACTTATTGAGGGCGCGGGGATTATGCTTAACAAGGTGC
TTAGCGGCCAGCAGCCGGTGCCGGCTATCATGGATGAACCAGTCTCTGGG
CATCCCAACGGTGGTGTTCCGGGGAAACAAGTTCCTCCGCCGGTTGGTTC
TGAAGCTAAACCGTGGTTTGGTGGTGCGTTGTTCGGTGAAGATAAGAAAA
AGGATGAACCTGTTTCTGGTGGAGGAAGTGAGACCAAGATTTTGGAGAGT
TTTGATGCTCCTCCTGTGCCAAATTTTGAGTACAAATGAAATCTGGTTTT
GGTTATTATGAATTATGAACCAAAGATCAAATATTTTGACAAAGGTTTTG
GTTTTTCTGAGCCTTTTCTACTGTATTTTACTTATAAGCAAGAGTTAGTT
TTATGTTGTATGATATTAATGCAAGAGAATGTTTACTAATTGGTGTGAAC
ATCTTATTGTGTGCAGGGTTTACAGTGTTTCCCCAGATATAGTTTGGGGA
GAAATATGTTATGGCATACACTTTTTTTTGTTTAGAAATGTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005743	mitochondrial inner membrane

Vocabulary: **Molecular Function**
Term	Definition
GO:0008565	obsolete protein transporter activity

Vocabulary: **Biological Process**
Term	Definition
GO:0015031	protein transport

Vocabulary: **INTERPRO**
Term	Definition
IPR003397	Tim17/Tim22/Tim23/PMP24

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