GT622322

EST Overview


Name	GT622322
Unique Name	GT622322
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	343

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT622322 vs. TrEMBL
Match: Q9LLQ6_SOYBN (Seed maturation protein PM34 OS=Glycine max GN=PM34 PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.707e-22
Identity = 51/55 (92.73%), Postives = 52/55 (94.55%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            FKEEETAQFG QVPM RAGQPIEVAPSYVFLAS+QCSSY TGQVLHPNGGTVVNG
Sbjct:  239 FKEEETAQFGAQVPMKRAGQPIEVAPSYVFLASNQCSSYITGQVLHPNGGTVVNG 293

[Close]

BLAST of GT622322 vs. TrEMBL
Match: C6TAX1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.707e-22
Identity = 51/55 (92.73%), Postives = 52/55 (94.55%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            FKEEETAQFG QVPM RAGQPIEVAPSYVFLAS+QCSSY TGQVLHPNGGTVVNG
Sbjct:  239 FKEEETAQFGAQVPMKRAGQPIEVAPSYVFLASNQCSSYITGQVLHPNGGTVVNG 293

[Close]

BLAST of GT622322 vs. TrEMBL
Match: C6T8W9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.029e-21
Identity = 50/55 (90.91%), Postives = 51/55 (92.73%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            FKEEETAQFG QVPM RAGQPIEVAPSYVFLA +QCSSY TGQVLHPNGGTVVNG
Sbjct:  239 FKEEETAQFGAQVPMKRAGQPIEVAPSYVFLACNQCSSYITGQVLHPNGGTVVNG 293

[Close]

BLAST of GT622322 vs. TrEMBL
Match: Q2HTL8_MEDTR (Short-chain dehydrogenase/reductase SDR OS=Medicago truncatula GN=MtrDRAFT_AC150244g25v2 PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.145e-19
Identity = 45/54 (83.33%), Postives = 49/54 (90.74%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE+TAQFG  VPM RAGQP+EVAPS+VFLAS+QCSSY TGQVLHPNGGTVVN
Sbjct:  239 FNEEKTAQFGSDVPMKRAGQPVEVAPSFVFLASNQCSSYITGQVLHPNGGTVVN 292

[Close]

BLAST of GT622322 vs. TrEMBL
Match: B9I7W9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_729630 PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.816e-18
Identity = 44/55 (80.00%), Postives = 46/55 (83.64%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            FKEEE A FG QVPM RAGQP EVAPSYVFLA + CSSY TGQVLHPNGG +VNG
Sbjct:  239 FKEEEVANFGKQVPMQRAGQPAEVAPSYVFLACNHCSSYITGQVLHPNGGVIVNG 293

[Close]

BLAST of GT622322 vs. TrEMBL
Match: Q8GSE7_LUPAN (Putative TAG factor protein OS=Lupinus angustifolius PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 6.901e-18
Identity = 44/55 (80.00%), Postives = 46/55 (83.64%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            FKEEETAQFG  VPM R GQP EVAPS+VFLA + CSSY TGQVLHPNGG VVNG
Sbjct:  240 FKEEETAQFGSDVPMKRPGQPSEVAPSFVFLACNICSSYITGQVLHPNGGNVVNG 294

[Close]

BLAST of GT622322 vs. TrEMBL
Match: D7KQ00_ARALY (Oxidoreductase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_314874 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.622e-17
Identity = 42/54 (77.78%), Postives = 45/54 (83.33%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE+   FG +VPM RAGQPIEVAPSYVFLA + CSSYFTGQVLHPNGG VVN
Sbjct:  280 FNEEKIKNFGSEVPMKRAGQPIEVAPSYVFLACNHCSSYFTGQVLHPNGGAVVN 333

[Close]

BLAST of GT622322 vs. TrEMBL
Match: B9RF47_RICCO (Short chain dehydrogenase, putative OS=Ricinus communis GN=RCOM_1431360 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.425e-17
Identity = 40/55 (72.73%), Postives = 45/55 (81.82%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            F EEE A FG +VPM RAGQP+EVAP YVFLA + CSSY TGQVLHPNGG ++NG
Sbjct:  239 FDEEEVANFGKEVPMQRAGQPVEVAPCYVFLACNHCSSYITGQVLHPNGGAIING 293

[Close]

BLAST of GT622322 vs. TrEMBL
Match: Q7DLK6_ARATH (NodG homolog (Fragment) OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.301e-16
Identity = 41/52 (78.85%), Postives = 44/52 (84.62%), Query Frame = -2

Query:  181 EEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 336
            EE+   FG +VPM RAGQPIEVAPSYVFLA + CSSYFTGQVLHPNGG VVN
Sbjct:    1 EEKIKNFGSEVPMKRAGQPIEVAPSYVFLACNHCSSYFTGQVLHPNGGAVVN 52

[Close]

BLAST of GT622322 vs. TrEMBL
Match: D7L3S3_ARALY (Short-chain dehydrogenase/reductase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_477813 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.102e-15
Identity = 37/54 (68.52%), Postives = 43/54 (79.63%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE   QFG + PM RA QP+EVAPSYVFLA + CSSY+TGQ+LHPNGG +VN
Sbjct:  237 FSEEAIKQFGSETPMKRAAQPVEVAPSYVFLACNHCSSYYTGQILHPNGGLIVN 290

[Close]

BLAST of GT622322 vs. SwissProt
Match: GRDH1_ARATH (Glucose and ribitol dehydrogenase homolog 1 OS=Arabidopsis thaliana GN=At1g54870 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.276e-18
Identity = 42/54 (77.78%), Postives = 45/54 (83.33%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE+   FG +VPM RAGQPIEVAPSYVFLA + CSSYFTGQVLHPNGG VVN
Sbjct:  234 FNEEKIKNFGSEVPMKRAGQPIEVAPSYVFLACNHCSSYFTGQVLHPNGGAVVN 287

[Close]

BLAST of GT622322 vs. SwissProt
Match: GRDH2_ARATH (Glucose and ribitol dehydrogenase homolog 2 OS=Arabidopsis thaliana GN=At3g05260 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.360e-17
Identity = 37/54 (68.52%), Postives = 43/54 (79.63%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE   QFG + PM RA QP+EVAPSYVFLA + CSSY+TGQ+LHPNGG +VN
Sbjct:  235 FSEEAIKQFGSETPMKRAAQPVEVAPSYVFLACNHCSSYYTGQILHPNGGLIVN 288

[Close]

BLAST of GT622322 vs. SwissProt
Match: GRDH_DAUCA (Glucose and ribitol dehydrogenase OS=Daucus carota GN=CAISE5 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.841e-15
Identity = 38/55 (69.09%), Postives = 44/55 (80.00%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            F EEE  QFG +VPM RAGQP E+A +YVFLAS   SSY++GQVLHPNGG +VNG
Sbjct:  238 FDEEEVKQFGSEVPMKRAGQPYEIATAYVFLASCD-SSYYSGQVLHPNGGAIVNG 291

[Close]

BLAST of GT622322 vs. SwissProt
Match: GRDH_ORYSJ (Glucose and ribitol dehydrogenase homolog OS=Oryza sativa subsp. japonica GN=Os05g0140800 PE=2 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 8.557e-15
Identity = 38/55 (69.09%), Postives = 44/55 (80.00%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            F EE+  QFG QVPM RAGQP EVAPS+VFLASD  +SY +GQ+LH NGG +VNG
Sbjct:  247 FAEEKVRQFGSQVPMGRAGQPSEVAPSFVFLASDD-ASYMSGQMLHVNGGVIVNG 300

[Close]

BLAST of GT622322 vs. SwissProt
Match: YHXC_BACSU (Uncharacterized oxidoreductase yhxC OS=Bacillus subtilis GN=yhxC PE=3 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 1.046e-12
Identity = 33/55 (60.00%), Postives = 41/55 (74.55%), Query Frame = -2

Query:  178 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVNG 342
            F  ++   FG  VPM R GQP+EVAPSY++LASD  S+Y TGQ +H NGGT+VNG
Sbjct:  232 FAAKDVEVFGSDVPMERPGQPVEVAPSYLYLASDD-STYVTGQTIHVNGGTIVNG 285

[Close]

BLAST of GT622322 vs. SwissProt
Match: GS39_BACSU (General stress protein 39 OS=Bacillus subtilis GN=ydaD PE=1 SV=3)

HSP 1 Score: 62.3882 bits (150), Expect = 8.288e-10
Identity = 32/53 (60.38%), Postives = 34/53 (64.15%), Query Frame = -2

Query:  184 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVV 342
            F EE  AQFG   PM R GQP+E    YV LASD+ SSY TGQ LH NGG  V
Sbjct:  233 FPEETVAQFGQDTPMGRPGQPVEHVGCYVLLASDE-SSYMTGQTLHVNGGNFV 284

[Close]

BLAST of GT622322 vs. SwissProt
Match: YAY8_SCHPO (Uncharacterized oxidoreductase C4H3.08 OS=Schizosaccharomyces pombe GN=SPAC4H3.08 PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.411e-9
Identity = 28/51 (54.90%), Postives = 34/51 (66.67%), Query Frame = -2

Query:  181 EETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 333
            +E  +   QVP+ R GQP+EVA  Y+FLA      Y TGQ LHPNGGTV+N
Sbjct:  236 KEKIELSDQVPLGRMGQPVEVASCYLFLACSD-GGYMTGQTLHPNGGTVIN 285

[Close]

BLAST of GT622322 vs. SwissProt
Match: YHDF_BACSU (Uncharacterized oxidoreductase yhdF OS=Bacillus subtilis GN=yhdF PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 9.163e-9
Identity = 29/54 (53.70%), Postives = 36/54 (66.67%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE+  Q G   PM R GQP+E A +YV LASD+ SSY TGQ +H NGG  ++
Sbjct:  236 FPEEKVKQHGLDTPMGRPGQPVEHAGAYVLLASDE-SSYMTGQTIHVNGGRFIS 288

[Close]

BLAST of GT622322 vs. TAIR peptide
Match: AT1G54870.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr1:20459011-20460417 FORWARD LENGTH=335)

HSP 1 Score: 91.6633 bits (226), Expect = 1.014e-19
Identity = 42/54 (77.78%), Postives = 45/54 (83.33%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE+   FG +VPM RAGQPIEVAPSYVFLA + CSSYFTGQVLHPNGG VVN
Sbjct:  281 FNEEKIKNFGSEVPMKRAGQPIEVAPSYVFLACNHCSSYFTGQVLHPNGGAVVN 334

[Close]

BLAST of GT622322 vs. TAIR peptide
Match: AT3G05260.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr3:1497665-1498919 REVERSE LENGTH=289)

HSP 1 Score: 86.2705 bits (212), Expect = 4.261e-18
Identity = 37/54 (68.52%), Postives = 43/54 (79.63%), Query Frame = -2

Query:  181 FKEEETAQFGGQVPMNRAGQPIEVAPSYVFLASDQCSSYFTGQVLHPNGGTVVN 342
            F EE   QFG + PM RA QP+EVAPSYVFLA + CSSY+TGQ+LHPNGG +VN
Sbjct:  235 FSEEAIKQFGSETPMKRAAQPVEVAPSYVFLACNHCSSYYTGQILHPNGGLIVN 288

The following BLAST results are available for this feature:

BLAST of GT622322 vs. TrEMBL
BLAST of GT622322 vs. SwissProt
BLAST of GT622322 vs. TAIR peptide

BLAST of GT622322 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9LLQ6_SOYBN	2.707e-22	92.73	Seed maturation protein PM34 OS=Glycine max GN=PM3...	[more]
C6TAX1_SOYBN	2.707e-22	92.73	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6T8W9_SOYBN	1.029e-21	90.91	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q2HTL8_MEDTR	2.145e-19	83.33	Short-chain dehydrogenase/reductase SDR OS=Medicag...	[more]
B9I7W9_POPTR	1.816e-18	80.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q8GSE7_LUPAN	6.901e-18	80.00	Putative TAG factor protein OS=Lupinus angustifoli...	[more]
D7KQ00_ARALY	2.622e-17	77.78	Oxidoreductase OS=Arabidopsis lyrata subsp. lyrata...	[more]
B9RF47_RICCO	3.425e-17	72.73	Short chain dehydrogenase, putative OS=Ricinus com...	[more]
Q7DLK6_ARATH	1.301e-16	78.85	NodG homolog (Fragment) OS=Arabidopsis thaliana PE...	[more]
D7L3S3_ARALY	1.102e-15	68.52	Short-chain dehydrogenase/reductase family protein...	[more]

BLAST of GT622322 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
GRDH1_ARATH	1.276e-18	77.78	Glucose and ribitol dehydrogenase homolog 1 OS=Ara...	[more]
GRDH2_ARATH	5.360e-17	68.52	Glucose and ribitol dehydrogenase homolog 2 OS=Ara...	[more]
GRDH_DAUCA	3.841e-15	69.09	Glucose and ribitol dehydrogenase OS=Daucus carota...	[more]
GRDH_ORYSJ	8.557e-15	69.09	Glucose and ribitol dehydrogenase homolog OS=Oryza...	[more]
YHXC_BACSU	1.046e-12	60.00	Uncharacterized oxidoreductase yhxC OS=Bacillus su...	[more]
GS39_BACSU	8.288e-10	60.38	General stress protein 39 OS=Bacillus subtilis GN=...	[more]
YAY8_SCHPO	2.411e-9	54.90	Uncharacterized oxidoreductase C4H3.08 OS=Schizosa...	[more]
YHDF_BACSU	9.163e-9	53.70	Uncharacterized oxidoreductase yhdF OS=Bacillus su...	[more]

BLAST of GT622322 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G54870.1	1.014e-19	77.78	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT3G05260.1	4.261e-18	68.52	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002198	Short-chain dehydrogenase/reductase SDR	PANTHER	PTHR19410	SHORT-CHAIN DEHYDROGENASES/REDUCTASE FAMILY MEMBER	coord: 1..49 score: 2
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 3..53 score: 2.4
None	No IPR available	PANTHER	PTHR19410:SF77	OXIDOREDUCTASE, SHORT CHAIN DEHYDROGENASE/REDUCTASE	coord: 1..49 score: 2
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..53 score: 1.9

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT622322 ID=GT622322; Name=GT622322; organism=Lens culinaris; type=EST; length=343bp
GATCTTAATACATAATGATCTGCATATTCTTTTACCTACATTTCTCCAAA
AATACATATTTATTTGCATAAAACTCTTTCACCCCAAACCCATCTCTTCA
CATTACATGGCACCGCTCTACTACTGCCCTACTGTTGTTTGTTCGTCTAT
TCATACAGTAATATTGCTAAATCTTCAACCATTGACTACGGTTCCACCAT
TGGGGTGAAGGACTTGTCCAGTAAAATAAGAGGAGCATTGATCAGAGGCA
AGAAAGACATAAGAGGGAGCAACCTCAATAGGTTGACCAGCTCTATTCAT
AGGAACTTGTCCACCAAATTGAGCAGTCTCTTCCTCCTTGAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002198	DH_sc/Rdtase_SDR
IPR016040	NAD(P)-bd_dom

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