GT622366

EST Overview


Name	GT622366
Unique Name	GT622366
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	699

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT622366 vs. TrEMBL
Match: A2Q3F3_MEDTR (Putative uncharacterized protein OS=Medicago truncatula GN=MtrDRAFT_AC155882g2v2 PE=2 SV=1)

HSP 1 Score: 355.91 bits (912), Expect = 1.754e-96
Identity = 174/176 (98.86%), Postives = 174/176 (98.86%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPS DNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISS PACSFVVRIKA
Sbjct:    1 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSTDNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSLPACSFVVRIKA 176

[Close]

BLAST of GT622366 vs. TrEMBL
Match: C6SZE5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 9.321e-90
Identity = 161/176 (91.48%), Postives = 168/176 (95.45%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            MVREVSESCVDSL+TEMVACYCNRFY NKPELAARRIEAIGYQVG QLSERYTMERPRF DHLEAIKFICKDFWSE+FKKQIDNLKTNHRGTFVLQDNKF WLARMS+DPS DNVS +ED ++PTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADIS+ PACSFVVRIKA
Sbjct:    1 MVREVSESCVDSLLTEMVACYCNRFYTNKPELAARRIEAIGYQVGHQLSERYTMERPRFTDHLEAIKFICKDFWSELFKKQIDNLKTNHRGTFVLQDNKFRWLARMSIDPSTDNVSPLEDITSPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISNLPACSFVVRIKA 176

[Close]

BLAST of GT622366 vs. TrEMBL
Match: C6TC59_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 330.872 bits (847), Expect = 6.042e-89
Identity = 159/176 (90.34%), Postives = 169/176 (96.02%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            MVREVSESC+DSL+TEMVACYCNRFYANKPELAARRIEAIGYQVG QLSERYTM+RPRF DHLEAIKFICKDFWSE+FKKQIDNLKTN+RGTFVLQDNKF WLARMS+DPS +NVS VED ++PTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADIS+ PACSFVVRIKA
Sbjct:    1 MVREVSESCIDSLLTEMVACYCNRFYANKPELAARRIEAIGYQVGHQLSERYTMDRPRFTDHLEAIKFICKDFWSELFKKQIDNLKTNYRGTFVLQDNKFRWLARMSIDPSTENVSPVEDITSPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISNLPACSFVVRIKA 176

[Close]

BLAST of GT622366 vs. TrEMBL
Match: B9REX0_RICCO (Trafficking protein particle complex subunit 6b, putative OS=Ricinus communis GN=RCOM_1429540 PE=4 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 6.056e-81
Identity = 150/176 (85.23%), Postives = 159/176 (90.34%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            M REVSESC+DSL+TEMV+ YCNRF ANKPELA RRIEAIGYQVG QL+ERYTMERPRF DHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKF WLARMS+DPS +N+   +D S    ESK+AQAMSMHLYFPCGIIRGALSNLGIPCAVSADISS PACSFVVRIKA
Sbjct:    1 MGREVSESCIDSLLTEMVSSYCNRFDANKPELAGRRIEAIGYQVGHQLAERYTMERPRFSDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFRWLARMSIDPSIENIGLSQDPSV-LEESKSAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSLPACSFVVRIKA 175

[Close]

BLAST of GT622366 vs. TrEMBL
Match: A5AGD5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_031270 PE=4 SV=1)

HSP 1 Score: 302.368 bits (773), Expect = 2.301e-80
Identity = 149/176 (84.66%), Postives = 160/176 (90.91%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            M REVSESCV+SL+TEMV+ YC RFYA+KP+LAARRIEAIGYQVG QLSERYTMERPRF DHLEAIKFICKDFWSE+FKKQIDNLKTNHRGTFVLQDNKF WL+RMSVDPS +N  S +D S  T E+KAAQA SMHLYFPCGIIRGALSNLGIPCAVSADIS+ PACSFVVRIKA
Sbjct:    1 MGREVSESCVESLLTEMVSSYCTRFYADKPDLAARRIEAIGYQVGHQLSERYTMERPRFSDHLEAIKFICKDFWSELFKKQIDNLKTNHRGTFVLQDNKFRWLSRMSVDPSPENTGSAQDPS--TDENKAAQATSMHLYFPCGIIRGALSNLGIPCAVSADISNLPACSFVVRIKA 174

[Close]

BLAST of GT622366 vs. TrEMBL
Match: A9PBG7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834509 PE=2 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 2.381e-77
Identity = 144/175 (82.29%), Postives = 154/175 (88.00%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            M REVSESC+DSL+TEMV+ YCNRFY+N PELAARRIEAIGYQVG QLSERYTMERPRF DHLEAIKFICKDFWSE+FKKQIDNLKTNH+GTFVLQDNKF WL+RMS DPS +N            ESKAAQAMSM+LYFPCGIIRGALSNLGIPCAVSADISS PACSFV+RIK
Sbjct:    1 MGREVSESCIDSLLTEMVSSYCNRFYSNTPELAARRIEAIGYQVGHQLSERYTMERPRFTDHLEAIKFICKDFWSELFKKQIDNLKTNHKGTFVLQDNKFRWLSRMSGDPSVEN-------GTQDPESKAAQAMSMYLYFPCGIIRGALSNLGIPCAVSADISSLPACSFVIRIK 168

[Close]

BLAST of GT622366 vs. TrEMBL
Match: Q9CAW4_ARATH (Putative uncharacterized protein At3g05000 OS=Arabidopsis thaliana GN=At3g05000 PE=2 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 5.305e-77
Identity = 143/176 (81.25%), Postives = 157/176 (89.20%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            M REV+ESCVD+++ EMVA Y  RFYANKPELAARRIEAIGYQVG QLSERYTMERPRF DHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKF WL+R+S+DPS++N    +D S P  ESKAAQA+SM+LYFPCGIIRG LSNLGIPCAVSADISS P CSFV+R+KA
Sbjct:    1 MGREVAESCVDTMLMEMVAMYSGRFYANKPELAARRIEAIGYQVGHQLSERYTMERPRFSDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFRWLSRVSIDPSSEN--ETQDPSTP-GESKAAQAVSMYLYFPCGIIRGVLSNLGIPCAVSADISSLPTCSFVIRVKA 173

[Close]

BLAST of GT622366 vs. TrEMBL
Match: B9MZL8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_827343 PE=4 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 1.035e-72
Identity = 140/177 (79.10%), Postives = 153/177 (86.44%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDP-SADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            M REVS+SC+D L+TEMV+ YC RFY+NKPELAARRIEAIGYQVG QLSERYT+ERPRF DHLEAIKFICKDFWSE+FKKQIDNLKTN RGTFVLQDNKFPWL+RMS D  S +N   +ED      ESK AQAMSM+ YFPCGIIRGALSNLGIPCAVSADISS PACSFV+ +KA
Sbjct:    1 MGREVSKSCMDGLVTEMVSSYCTRFYSNKPELAARRIEAIGYQVGHQLSERYTIERPRFTDHLEAIKFICKDFWSELFKKQIDNLKTNRRGTFVLQDNKFPWLSRMSGDDLSVEN--GMED-----PESKGAQAMSMYCYFPCGIIRGALSNLGIPCAVSADISSLPACSFVISVKA 170

[Close]

BLAST of GT622366 vs. TrEMBL
Match: Q6K335_ORYSJ (Putative trafficking protein particle complex 6B OS=Oryza sativa subsp. japonica GN=OSJNBa0069P02.11-1 PE=2 SV=1)

HSP 1 Score: 274.248 bits (700), Expect = 6.711e-72
Identity = 132/175 (75.43%), Postives = 153/175 (87.43%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            MVREV+ESCVD ++ EMVA YC RFYA KPELAARRIEAIG+QVG QL+ERYTMERPRF DHLEAIKFICKDFWSE+FKKQIDNLKTNHRGTFVLQDN+F WL R+S+DPS +++ + ++ SA   +S AAQ  SM LYFPCGIIRGAL+NLGI C+V+AD+S+ PACSFVVRIK
Sbjct:    1 MVREVAESCVDGVVMEMVAAYCGRFYAAKPELAARRIEAIGFQVGHQLTERYTMERPRFSDHLEAIKFICKDFWSELFKKQIDNLKTNHRGTFVLQDNRFRWLTRVSIDPSVESMDATDNDSATLGDS-AAQTTSMLLYFPCGIIRGALTNLGISCSVTADMSNLPACSFVVRIK 174

[Close]

BLAST of GT622366 vs. TrEMBL
Match: A9NV50_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 5.681e-71
Identity = 131/176 (74.43%), Postives = 150/176 (85.23%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVD-PSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            MVREVSESCVDS+  EMVA YC RFYANK ELAARRIEAIGYQVG QL+ERYT ERPRF DHLEAIKFICKDFWSE+FKKQIDNLKTNHRGTFVLQDN+F WL R+S+D P      S ++ S  + ++K+AQA  MHLYFPCGIIRGAL+NLG+PC VSA++++ PACSFV+RIK
Sbjct:    1 MVREVSESCVDSVTMEMVAAYCGRFYANKQELAARRIEAIGYQVGLQLAERYTKERPRFADHLEAIKFICKDFWSELFKKQIDNLKTNHRGTFVLQDNRFRWLTRISLDSPLPTANGSPKESSTTSNDAKSAQAAGMHLYFPCGIIRGALTNLGVPCQVSAEVANLPACSFVIRIK 176

[Close]

BLAST of GT622366 vs. SwissProt
Match: TPC6B_HUMAN (Trafficking protein particle complex subunit 6B OS=Homo sapiens GN=TRAPPC6B PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 5.652e-31
Identity = 69/140 (49.29%), Postives = 91/140 (65.00%), Query Frame = 3

Query:  150 RIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            ++E +G++VGQ L ER+T +  RF D L+ +KFICKDFW+ VFKKQIDNL+TNH+G +VLQDNKF  L +MS                  A  +  +  S +L F CG+IRG LSNLGI   V+A++SS PAC F V I+
Sbjct:   35 KLENMGFRVGQGLIERFTKDTARFKDELDIMKFICKDFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLTQMS------------------AGKQYLEHASKYLAFTCGLIRGGLSNLGIKSIVTAEVSSMPACKFQVMIQ 156

[Close]

BLAST of GT622366 vs. SwissProt
Match: TPC6B_BOVIN (Trafficking protein particle complex subunit 6B OS=Bos taurus GN=TRAPPC6B PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 5.652e-31
Identity = 69/140 (49.29%), Postives = 91/140 (65.00%), Query Frame = 3

Query:  150 RIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            ++E +G++VGQ L ER+T +  RF D L+ +KFICKDFW+ VFKKQIDNL+TNH+G +VLQDNKF  L +MS                  A  +  +  S +L F CG+IRG LSNLGI   V+A++SS PAC F V I+
Sbjct:   35 KLENMGFRVGQGLIERFTKDTARFKDELDIMKFICKDFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLTQMS------------------AGKQYLEHASKYLAFTCGLIRGGLSNLGIKSIVTAEVSSMPACKFQVMIQ 156

[Close]

BLAST of GT622366 vs. SwissProt
Match: TPC6B_MOUSE (Trafficking protein particle complex subunit 6B OS=Mus musculus GN=Trappc6b PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 7.381e-31
Identity = 68/140 (48.57%), Postives = 92/140 (65.71%), Query Frame = 3

Query:  150 RIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            ++E++G++VGQ L ER+T +  RF D L+ +KFICKDFW+ VFKKQIDNL+TNH+G +VLQDNKF  L ++S                  A  +  +  S +L F CG+IRG LSNLGI   V+A++SS PAC F V I+
Sbjct:   35 KLESMGFRVGQGLIERFTKDTARFKDELDIMKFICKDFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLIQLS------------------AGKQYLEHASKYLAFTCGLIRGGLSNLGIKSIVTAEVSSMPACKFQVMIQ 156

[Close]

BLAST of GT622366 vs. SwissProt
Match: TPC6A_BOVIN (Trafficking protein particle complex subunit 6A OS=Bos taurus GN=TRAPPC6A PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.348e-24
Identity = 60/139 (43.17%), Postives = 84/139 (60.43%), Query Frame = 3

Query:  153 IEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            +E +G++VGQ L ER   E   F + L+ +KF+CKD W  VF KQ+D+L+TNH+GT+VLQDN FP L RM     A  +  +E+  AP             L F CG++RG LS LG+   V+A +++ PAC F V I+
Sbjct:   37 LEGMGFRVGQALGERLPRETLTFREELDILKFLCKDLWVAVFHKQMDSLRTNHQGTYVLQDNSFPLLVRM-----ASGLQYLEE--AP-----------KFLAFTCGLLRGTLSTLGVKSLVTASVAALPACKFQVVIQ 157

[Close]

BLAST of GT622366 vs. SwissProt
Match: TPC6A_MOUSE (Trafficking protein particle complex subunit 6A OS=Mus musculus GN=Trappc6a PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.761e-24
Identity = 65/170 (38.24%), Postives = 93/170 (54.71%), Query Frame = 3

Query:   81 LMTEMVACYCNRFYANKPELAA---RR----IEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIK 569
            L TEMVA      +A  P+  +   RR    +E +G++VGQ L ER  +E P F + L+A+KF+C+D W+ +F+K +D L+TNH+GT+VLQDN FP L  M   P                  +  +     L F CG++ GAL  LG    V+A ++S PAC F V I+
Sbjct:   10 LHTEMVA----ELWAPDPDPGSGGKRRSLSVLEGLGFRVGQALGERLPLETPAFREELDALKFLCRDLWAAMFQKHMDGLRTNHQGTYVLQDNSFPLLVTMGSGP------------------QYLEEAPKFLAFTCGLLCGALHTLGFQSLVTASVASLPACKFQVVIQ 157

[Close]

BLAST of GT622366 vs. SwissProt
Match: TPC6A_HUMAN (Trafficking protein particle complex subunit 6A OS=Homo sapiens GN=TRAPPC6A PE=1 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 2.300e-24
Identity = 67/169 (39.64%), Postives = 95/169 (56.21%), Query Frame = 3

Query:   81 LMTEMVACYCNRFYANKPE-------LAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRI 566
            L TEMVA      +A+ P+       ++   +E +G++VGQ L ER   E   F + L+ +KF+CKD W  VF+KQ+D+L+TNH+GT+VLQDN FP L      P A  +  +E+  AP             L F CG++RGAL  LGI   V+A +++ P C F V I
Sbjct:   10 LHTEMVA----ELWAHDPDPGPGGQKMSLSVLEGMGFRVGQALGERLPRETLAFREELDVLKFLCKDLWVAVFQKQMDSLRTNHQGTYVLQDNSFPLLL-----PMASGLQYLEE--AP-----------KFLAFTCGLLRGALYTLGIESVVTASVAALPVCKFQVVI 156

[Close]

BLAST of GT622366 vs. SwissProt
Match: YA95_SCHPO (Uncharacterized protein C13G6.05c OS=Schizosaccharomyces pombe GN=SPAC13G6.05c PE=1 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 2.228e-19
Identity = 47/137 (34.31%), Postives = 73/137 (53.28%), Query Frame = 3

Query:  153 IEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVR 563
            +E+IG+QVG++++ER  + R R  +  + ++F+C++ W  VF+K +DNLKTN RG FVL D  F W  +M+                    ++ AQ  + + YFP G IRG +   G    V A   + P C F V+
Sbjct:   43 LESIGFQVGRKITERLLLNRNRITETTDVMRFLCRELWPIVFRKPLDNLKTNRRGIFVLTDTYFYWFTKMTA----------------MTGTEMAQITTPYFYFPSGFIRGVVYTFGYSAQVIAQCPNLPTCIFHVK 163

[Close]

BLAST of GT622366 vs. SwissProt
Match: TRS33_YEAST (Transport protein particle 33 kDa subunit OS=Saccharomyces cerevisiae GN=TRS33 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 4.807e-14
Identity = 50/140 (35.71%), Postives = 69/140 (49.29%), Query Frame = 3

Query:  135 ELAARRIEAIGYQVGQQLSERYTMERP-----RFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSF 539
            E    R+  IG+Q+G +LSE            +  D L  +KFIC+D W ++F KQIDNLKTNHRGTF L D  +  +   S++  A N              +  + +   L  P GIIRG LS+LG        ++SF
Sbjct:  117 EKVLARLRNIGFQIGLKLSELLIFSNNPNLKFKEMDLLLIMKFICRDVWKQIFGKQIDNLKTNHRGTFYLLDYDYRPIQSFSLEEDAKN--------------EELKMIEPFLEIPVGIIRGVLSSLGYSSEEVICLASF 242

[Close]

BLAST of GT622366 vs. TAIR peptide
Match: AT3G05000.1 (| Symbols: | Transport protein particle (TRAPP) component | chr3:1387444-1388697 REVERSE LENGTH=173)

HSP 1 Score: 291.197 bits (744), Expect = 3.635e-79
Identity = 143/176 (81.25%), Postives = 157/176 (89.20%), Query Frame = 3

Query:   45 MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSADNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSFPACSFVVRIKA 572
            M REV+ESCVD+++ EMVA Y  RFYANKPELAARRIEAIGYQVG QLSERYTMERPRF DHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKF WL+R+S+DPS++N    +D S P  ESKAAQA+SM+LYFPCGIIRG LSNLGIPCAVSADISS P CSFV+R+KA
Sbjct:    1 MGREVAESCVDTMLMEMVAMYSGRFYANKPELAARRIEAIGYQVGHQLSERYTMERPRFSDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFRWLSRVSIDPSSEN--ETQDPSTP-GESKAAQAVSMYLYFPCGIIRGVLSNLGIPCAVSADISSLPTCSFVIRVKA 173

The following BLAST results are available for this feature:

BLAST of GT622366 vs. TrEMBL
BLAST of GT622366 vs. SwissProt
BLAST of GT622366 vs. TAIR peptide

BLAST of GT622366 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A2Q3F3_MEDTR	1.754e-96	98.86	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6SZE5_SOYBN	9.321e-90	91.48	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TC59_SOYBN	6.042e-89	90.34	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9REX0_RICCO	6.056e-81	85.23	Trafficking protein particle complex subunit 6b, p...	[more]
A5AGD5_VITVI	2.301e-80	84.66	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A9PBG7_POPTR	2.381e-77	82.29	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9CAW4_ARATH	5.305e-77	81.25	Putative uncharacterized protein At3g05000 OS=Arab...	[more]
B9MZL8_POPTR	1.035e-72	79.10	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q6K335_ORYSJ	6.711e-72	75.43	Putative trafficking protein particle complex 6B O...	[more]
A9NV50_PICSI	5.681e-71	74.43	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of GT622366 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
TPC6B_HUMAN	5.652e-31	49.29	Trafficking protein particle complex subunit 6B OS...	[more]
TPC6B_BOVIN	5.652e-31	49.29	Trafficking protein particle complex subunit 6B OS...	[more]
TPC6B_MOUSE	7.381e-31	48.57	Trafficking protein particle complex subunit 6B OS...	[more]
TPC6A_BOVIN	1.348e-24	43.17	Trafficking protein particle complex subunit 6A OS...	[more]
TPC6A_MOUSE	1.761e-24	38.24	Trafficking protein particle complex subunit 6A OS...	[more]
TPC6A_HUMAN	2.300e-24	39.64	Trafficking protein particle complex subunit 6A OS...	[more]
YA95_SCHPO	2.228e-19	34.31	Uncharacterized protein C13G6.05c OS=Schizosacchar...	[more]
TRS33_YEAST	4.807e-14	35.71	Transport protein particle 33 kDa subunit OS=Sacch...	[more]

BLAST of GT622366 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G05000.1	3.635e-79	81.25	\| Symbols: \| Transport protein particle (TRAPP) c...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007194	Transport protein particle (TRAPP) component	PFAM	PF04051	TRAPP	coord: 23..189 score: 1.2
IPR011644	Haem NO binding	SUPERFAMILY	111126	Ligand-binding domain in the NO signalling and Golgi transport	coord: 17..188 score: 2.1
None	No IPR available	GENE3D	3.30.1380.20	no description	coord: 18..185 score: 2.5
None	No IPR available	PANTHER	PTHR12817	TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B	coord: 11..190 score: 4.9
None	No IPR available	SEG	seg	seg	coord: 1..14 score
None	No IPR available	SEG	seg	seg	coord: 59..80 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT622366 ID=GT622366; Name=GT622366; organism=Lens culinaris; type=EST; length=699bp
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATGGTG
AGGGAAGTTTCAGAGAGTTGCGTCGATAGCTTGATGACAGAAATGGTTGC
GTGCTATTGCAATCGGTTCTACGCCAACAAACCTGAACTCGCTGCCCGGA
GGATCGAAGCCATTGGTTATCAGGTCGGTCAACAGCTCTCTGAAAGATAT
ACGATGGAACGGCCTCGGTTTGGTGATCATTTGGAAGCAATCAAGTTTAT
TTGTAAGGATTTTTGGTCTGAGGTATTTAAGAAGCAAATTGACAACTTGA
AAACTAACCATAGGGGTACATTTGTATTGCAAGATAATAAATTTCCCTGG
CTTGCACGGATGTCGGTTGATCCATCCGCTGATAATGTTAGTTCAGTTGA
GGATTATTCTGCACCTACAGCTGAAAGTAAGGCAGCACAGGCAATGAGCA
TGCATCTGTATTTCCCGTGTGGGATTATTAGAGGAGCTCTTTCCAATTTG
GGAATTCCCTGTGCCGTTTCTGCAGACATATCAAGCTTTCCAGCATGTTC
ATTCGTGGTCCGTATAAAAGCTTGATAAATTTGTGCTATGCTGCGAGACT
AGCAACATTGTTCGGAACAATCGTTTGGTGTCTGCGATTGTGCTCTTTGG
CACCAAATTTGTTCTTTATCATATTTAAACTATCAAATACTATTGACTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004383	guanylate cyclase activity
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006182	cGMP biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR007194	TRAPP_component
IPR011644	Heme_NO-bd

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