GT623127

EST Overview


Name	GT623127
Unique Name	GT623127
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	680

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT623127 vs. TrEMBL
Match: B9GH97_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_549896 PE=3 SV=1)

HSP 1 Score: 236.498 bits (602), Expect = 1.480e-60
Identity = 116/170 (68.24%), Postives = 134/170 (78.82%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            TTPLI +P+ PSFYYLSLEGISVGDTRL I+KSTF +                 Y+EE+AF  + KEF ++ NLPVD SGSTGLDVCF+LPSG T +E+PKLVFHF G DLEL  ENYMIGDSS+GVACLAMG+S GMSIFGNVQQQN+LV HDLEKET+SF+PTQC+ L
Sbjct:  270 TTPLIHSPAHPSFYYLSLEGISVGDTRLPIKKSTFSLQDDGSGGLIIDSGTTITYLEESAFNLVAKEFTAKINLPVDSSGSTGLDVCFTLPSGSTNIEVPKLVFHFDGADLELPAENYMIGDSSMGVACLAMGSSSGMSIFGNVQQQNMLVLHDLEKETLSFLPTQCDLL 439

[Close]

BLAST of GT623127 vs. TrEMBL
Match: B9N2J4_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_264367 PE=3 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 2.137e-59
Identity = 113/170 (66.47%), Postives = 134/170 (78.82%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            TTPLI N +QPSFYYLSLEGISVGDT L I+KSTF +                 Y+E++AF  + KEF SQ NLPVD+SGSTGL+VCF+LPSG T +E+PKLVFHF G DLEL  ENYMI D+S+GVACLAMG+S GMSIFGN+QQQN+LV HDLEKET+SF+PTQC++L
Sbjct:  270 TTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLQEDGSGGLIIDSGTTITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPSGSTDIEVPKLVFHFDGADLELPAENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 439

[Close]

BLAST of GT623127 vs. TrEMBL
Match: B9SA95_RICCO (Aspartic proteinase nepenthesin-1, putative OS=Ricinus communis GN=RCOM_1697100 PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.694e-56
Identity = 108/170 (63.53%), Postives = 132/170 (77.65%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            TTPLI NP QPSFYYLSLEGISVG TRL I++STF++                 Y+EE+AF  +KKEF SQ  LPVD+SG+TGL++C++LPS  +++E+PKLV HF G DLEL GENYMI DSS+GV CLAMG+S GMSIFGNVQQQN+ V+HDLEKET+SF+PT C +L
Sbjct:  273 TTPLIQNPLQPSFYYLSLEGISVGGTRLPIKESTFQLQDDGTGGLIIDSGTTITYLEESAFDLVKKEFTSQMGLPVDNSGATGLELCYNLPSDTSELEVPKLVLHFTGADLELPGENYMIADSSMGVICLAMGSSGGMSIFGNVQQQNMFVSHDLEKETLSFLPTNCGQL 442

[Close]

BLAST of GT623127 vs. TrEMBL
Match: Q84M99_ARATH (At2g03200 OS=Arabidopsis thaliana GN=At2g03200 PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.873e-55
Identity = 109/175 (62.29%), Postives = 127/175 (72.57%), Query Frame = 2

Query:   14 VTKHVTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            VTK  T  L+ NP QPSFYYL L+GI+VG  RLS+EKSTFE+                 Y+EE AF  LK+EF S+ +LPVDDSGSTGLD+CF LP     + +PK++FHFKG DLEL GENYM+ DSS GV CLAMG+S GMSIFGNVQQQN  V HDLEKET+SFVPT+C KL
Sbjct:  289 VTK--TMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

[Close]

BLAST of GT623127 vs. TrEMBL
Match: O81054_ARATH (Putative chloroplast nucleoid DNA binding protein OS=Arabidopsis thaliana GN=At2g03200 PE=2 SV=2)

HSP 1 Score: 219.55 bits (558), Expect = 1.873e-55
Identity = 109/175 (62.29%), Postives = 127/175 (72.57%), Query Frame = 2

Query:   14 VTKHVTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            VTK  T  L+ NP QPSFYYL L+GI+VG  RLS+EKSTFE+                 Y+EE AF  LK+EF S+ +LPVDDSGSTGLD+CF LP     + +PK++FHFKG DLEL GENYM+ DSS GV CLAMG+S GMSIFGNVQQQN  V HDLEKET+SFVPT+C KL
Sbjct:  181 VTK--TMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 353

[Close]

BLAST of GT623127 vs. TrEMBL
Match: D7LQF2_ARALY (Aspartyl protease family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_904590 PE=4 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.873e-55
Identity = 109/175 (62.29%), Postives = 128/175 (73.14%), Query Frame = 2

Query:   14 VTKHVTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            VTK  T  L+ NP QPSFYYL L+GI+VG  RLS+EKSTFE+                 Y+EE AF  LK+EF S+ +LPVDDSGSTGLD+CF LP+    + +PKL+FHFKG DLEL GENYM+ DSS GV CLAMG+S GMSIFGNVQQQN  V HDLEKET++FVPT+C KL
Sbjct:  290 VTK--TMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELSEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTGLDLCFKLPNAAKNIAVPKLIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGMSIFGNVQQQNFNVLHDLEKETVTFVPTECGKL 462

[Close]

BLAST of GT623127 vs. TrEMBL
Match: A5BUH7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006338 PE=3 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 2.289e-53
Identity = 108/175 (61.71%), Postives = 128/175 (73.14%), Query Frame = 2

Query:   14 VTKHVTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            V   + TPLI NPS+PSFYYLSLEGISVGDT L IEKSTF +                 Y+++NAF +LKKEFISQ  L VD SGST L++CF+LP   + VE+P+LVFHF+G DL+L  ENY+I DS+L V CL MG+S GMSIFGN QQQNI+V HDLEKETISF P QCN+L
Sbjct:  262 VKSAIPTPLIQNPSRPSFYYLSLEGISVGDTLLPIEKSTFSIQDDGSGGLIIDSGTTITYLKDNAFAALKKEFISQMKLDVDASGSTELELCFTLPPDGSPVEVPQLVFHFEGVDLKLPKENYIIEDSALRVICLTMGSSSGMSIFGNFQQQNIVVLHDLEKETISFAPAQCNQL 436

[Close]

BLAST of GT623127 vs. TrEMBL
Match: Q7XV20_ORYSJ (OSJNBa0064H22.10 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0064H22.10 PE=3 SV=2)

HSP 1 Score: 163.31 bits (412), Expect = 1.593e-38
Identity = 84/172 (48.84%), Postives = 106/172 (61.63%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPS-GKTQVEIPKLVFHFKGG-DLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            TTPLI NPSQPSFYY+SL+ I+VG TR+S+  S F V                 Y+E   + +LKK F +Q  LP  D    GLD+CF  P+ G  QVE+P+LVFHF GG DL+L  ENYM+ D   G  CL +  S+G+SI GN QQQN    +D+  +T+SF P QCNKL
Sbjct:  273 TTPLIKNPSQPSFYYVSLKAITVGSTRISLPSSAFAVQDDGTGGVIVDSGTSITYLEVQGYRALKKAFAAQMALPAADGSGVGLDLCFRAPAKGVDQVEVPRLVFHFDGGADLDLPAENYMVLDGGSGALCLTVMGSRGLSIIGNFQQQNFQFVYDVGHDTLSFAPVQCNKL 444

[Close]

BLAST of GT623127 vs. TrEMBL
Match: Q0JCV1_ORYSJ (Os04g0448300 protein OS=Oryza sativa subsp. japonica GN=Os04g0448300 PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.593e-38
Identity = 84/172 (48.84%), Postives = 106/172 (61.63%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPS-GKTQVEIPKLVFHFKGG-DLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            TTPLI NPSQPSFYY+SL+ I+VG TR+S+  S F V                 Y+E   + +LKK F +Q  LP  D    GLD+CF  P+ G  QVE+P+LVFHF GG DL+L  ENYM+ D   G  CL +  S+G+SI GN QQQN    +D+  +T+SF P QCNKL
Sbjct:  283 TTPLIKNPSQPSFYYVSLKAITVGSTRISLPSSAFAVQDDGTGGVIVDSGTSITYLEVQGYRALKKAFAAQMALPAADGSGVGLDLCFRAPAKGVDQVEVPRLVFHFDGGADLDLPAENYMVLDGGSGALCLTVMGSRGLSIIGNFQQQNFQFVYDVGHDTLSFAPVQCNKL 454

[Close]

BLAST of GT623127 vs. TrEMBL
Match: Q01J77_ORYSA (H0818E04.1 protein OS=Oryza sativa GN=OSIGBa0152K17.10 PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.593e-38
Identity = 84/172 (48.84%), Postives = 106/172 (61.63%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPS-GKTQVEIPKLVFHFKGG-DLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            TTPLI NPSQPSFYY+SL+ I+VG TR+S+  S F V                 Y+E   + +LKK F +Q  LP  D    GLD+CF  P+ G  QVE+P+LVFHF GG DL+L  ENYM+ D   G  CL +  S+G+SI GN QQQN    +D+  +T+SF P QCNKL
Sbjct:  273 TTPLIKNPSQPSFYYVSLKAITVGSTRISLPSSAFAVQDDGTGGVIVDSGTSITYLEVQGYRALKKAFAAQMALPAADGSGVGLDLCFRAPAKGVDQVEVPRLVFHFDGGADLDLPAENYMVLDGGSGALCLTVMGSRGLSIIGNFQQQNFQFVYDVGHDTLSFAPVQCNKL 444

[Close]

BLAST of GT623127 vs. SwissProt
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.612e-35
Identity = 80/168 (47.62%), Postives = 104/168 (61.90%), Query Frame = 2

Query:   32 TPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXX-YIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGAS-QGMSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            T LI +   P+FYY++L G+SVG TRL I+ S F +                  Y   NA+ S+++EFISQ NLPV +  S+G D+CF  PS  + ++IP  V HF GGDLEL  ENY I  S+ G+ CLAMG+S QGMSIFGN+QQQN+LV +D     +SF   QC
Sbjct:  268 TTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPSN-GLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of GT623127 vs. SwissProt
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.726e-29
Identity = 67/169 (39.64%), Postives = 99/169 (58.58%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQ--GMSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            +T LI +   P++YY++L+GI+VG   L I  STF++                 Y+ ++A+ ++ + F  Q NLP  D  S+GL  CF  PS  + V++P++   F GG L L  +N +I  +  GV CLAMG+S   G+SIFGN+QQQ   V +DL+   +SFVPTQC
Sbjct:  268 STTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT2G03200.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr2:966506-967891 REVERSE LENGTH=461)

HSP 1 Score: 219.55 bits (558), Expect = 1.284e-57
Identity = 109/175 (62.29%), Postives = 127/175 (72.57%), Query Frame = 2

Query:   14 VTKHVTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            VTK  T  L+ NP QPSFYYL L+GI+VG  RLS+EKSTFE+                 Y+EE AF  LK+EF S+ +LPVDDSGSTGLD+CF LP     + +PK++FHFKG DLEL GENYM+ DSS GV CLAMG+S GMSIFGNVQQQN  V HDLEKET+SFVPT+C KL
Sbjct:  289 VTK--TMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT1G25510.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr1:8959372-8960823 REVERSE LENGTH=483)

HSP 1 Score: 99.7525 bits (247), Expect = 1.483e-21
Identity = 62/170 (36.47%), Postives = 83/170 (48.82%), Query Frame = 2

Query:   26 VTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGD-LELAGENYMIGDSSLGVACLAMG-ASQGMSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            V  PL+ N    +FYYL L GISVG   L I +S+FE+                  ++   + SL+  F+  T      +G    D C++L S KT VE+P + FHF GG  L L  +NYMI   S+G  CLA    +  ++I GNVQQQ   V  DL    I F   +C
Sbjct:  315 VVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNL-SAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT3G61820.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:22880074-22881525 REVERSE LENGTH=483)

HSP 1 Score: 97.0561 bits (240), Expect = 9.614e-21
Identity = 62/171 (36.26%), Postives = 90/171 (52.63%), Query Frame = 2

Query:   26 VTTPLITNPSQPSFYYLSLEGISVGDTRL-SIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEF-ISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQG-MSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            V TPL+TNP   +FYYL L GISVG +R+  + +S F++                  + + A+ +L+  F +  T L    S S   D CF L SG T V++P +VFHF GG++ L   NY+I  ++ G  C A   + G +SI GN+QQQ   V +DL    + F+   C
Sbjct:  315 VFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSL-FDTCFDL-SGMTTVKVPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT3G18490.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:6349090-6350592 REVERSE LENGTH=500)

HSP 1 Score: 89.7373 bits (221), Expect = 1.535e-18
Identity = 55/170 (32.35%), Postives = 80/170 (47.06%), Query Frame = 2

Query:   29 TTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQT-NLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGG-DLELAGENYMIGDSSLGVACLAMG-ASQGMSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            T PL+ N    +FYY+ L G SVG  ++ +  + F+V                  ++  A+ SL+  F+  T NL    S  +  D C+   S  T V++P + FHF GG  L+L  +NY+I     G  C A    S  +SI GNVQQQ   + +DL K  I     +C
Sbjct:  332 TAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT1G01300.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr1:117065-118522 FORWARD LENGTH=485)

HSP 1 Score: 87.0409 bits (214), Expect = 9.949e-18
Identity = 54/168 (32.14%), Postives = 80/168 (47.62%), Query Frame = 2

Query:   32 TPLITNPSQPSFYYLSLEGISVGDTRL-SIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAM-GASQGMSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            TPL++NP   +FYY+ L GISVG TR+  +  S F++                  +   A+ +++  F             +  D CF L S   +V++P +V HF+G D+ L   NY+I   + G  C A  G   G+SI GN+QQQ   V +DL    + F P  C
Sbjct:  318 TPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDL-SNMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT5G33340.1 (| Symbols: CDR1 | Eukaryotic aspartyl protease family protein | chr5:12594474-12595787 FORWARD LENGTH=437)

HSP 1 Score: 85.1149 bits (209), Expect = 3.781e-17
Identity = 52/171 (30.41%), Postives = 78/171 (45.61%), Query Frame = 2

Query:   26 VTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAMGASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCNKL 538
            V+TPLI   SQ +FYYL+L+ ISVG  ++    S  E                   +    +  L+    S  +        +GL +C+S       +++P +  HF G D++L   N  +  S   + C A   S   SI+GNV Q N LV +D   +T+SF PT C K+
Sbjct:  274 VSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSATG---DLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDCAKM 437

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT3G20015.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:6978746-6980158 REVERSE LENGTH=470)

HSP 1 Score: 85.1149 bits (209), Expect = 3.781e-17
Identity = 56/168 (33.33%), Postives = 82/168 (48.81%), Query Frame = 2

Query:   35 PLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQT-NLPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHF-KGGDLELAGENYMIGDSSLGVACLAMGAS-QGMSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            PL+ NP  PSFYY+ L+G+ VG  R+ +    F++                  +   A+ + +  F SQT NLP   SG +  D C+ L SG   V +P + F+F +G  L L   N+++     G  C A  AS  G+SI GN+QQ+ I V+ D     + F P  C
Sbjct:  305 PLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLP-RASGVSIFDTCYDL-SGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT3G25700.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:9358937-9360295 FORWARD LENGTH=452)

HSP 1 Score: 80.8777 bits (198), Expect = 7.130e-16
Identity = 52/178 (29.21%), Postives = 89/178 (50.00%), Query Frame = 2

Query:   14 VTKHVTTPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGSTGLDVCFSLPSGKTQVE--IPKLVFHFKGGDLEL-AGENYMIGDSSLGVACLAMGA---SQGMSIFGNVQQQNILVNHDLEKETISFVPTQC 529
            ++K   TPL+TNP  P+FYY+ L+ + V   +L I+ S +E+                 ++ E A+ S+      +  LP+ D+ + G D+C ++ SG T+ E  +P+L F F GG + +    NY I ++   + CLA+ +     G S+ GN+ QQ  L   D ++  + F    C
Sbjct:  274 ISKLFFTPLLTNPLSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIADALTPGFDLCVNV-SGVTKPEKILPRLKFEFSGGAVFVPPPRNYFI-ETEEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGC 449

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT1G09750.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr1:3157541-3158960 FORWARD LENGTH=449)

HSP 1 Score: 75.485 bits (184), Expect = 2.996e-14
Identity = 49/174 (28.16%), Postives = 79/174 (45.40%), Query Frame = 2

Query:   32 TPLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTNLPVDDSGST--GLDVCFSLPSGKTQVEIPKLVFHFKGGDLELAGENYMIGDSSLGVACLAM-----GASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCN 532
            TPL+ NP +PS YY++L G+SVG  ++ ++                          +  + +++ EF  Q N+    S ST    D CFS  +       PK+  H    DL+L  EN +I  S+  + CL+M      A+  +++  N+QQQN+ +  D+    I   P  CN
Sbjct:  282 TPLLRNPRRPSLYYVNLTGVSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNV---SSFSTLGAFDTCFSADNENV---APKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of GT623127 vs. TAIR peptide
Match: AT3G52500.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:19465644-19467053 REVERSE LENGTH=469)

HSP 1 Score: 72.7886 bits (177), Expect = 1.942e-13
Identity = 54/179 (30.17%), Postives = 78/179 (43.58%), Query Frame = 2

Query:   35 PLITNPSQPSFYYLSLEGISVGDTRLSIEKSTFEVXXXXXXXXXXXXXXXXXYIEENAFGSLKKEFISQTN---LPVDDSGSTGLDVCFSLPSGKTQVEIPKLVFHFKGG-DLELAGENYMIGDSSLGVACLAM---------GASQGMSIFGNVQQQNILVNHDLEKETISFVPTQCN 532
            P ++N +   +YYL+L  I VG   + I                        ++E   F  + +EF SQ +      D    TGL  CF++ SGK  V +P+L+F FKGG  LEL   NY     +    CL +         G +    I G+ QQQN LV +DLE +   F   +C+
Sbjct:  291 PNVSNKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQMSNYTREKDLEKETGLGPCFNI-SGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNTDTVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

The following BLAST results are available for this feature:

BLAST of GT623127 vs. TrEMBL
BLAST of GT623127 vs. SwissProt
BLAST of GT623127 vs. TAIR peptide

BLAST of GT623127 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9GH97_POPTR	1.480e-60	68.24	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9N2J4_POPTR	2.137e-59	66.47	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9SA95_RICCO	1.694e-56	63.53	Aspartic proteinase nepenthesin-1, putative OS=Ric...	[more]
Q84M99_ARATH	1.873e-55	62.29	At2g03200 OS=Arabidopsis thaliana GN=At2g03200 PE=...	[more]
O81054_ARATH	1.873e-55	62.29	Putative chloroplast nucleoid DNA binding protein ...	[more]
D7LQF2_ARALY	1.873e-55	62.29	Aspartyl protease family protein OS=Arabidopsis ly...	[more]
A5BUH7_VITVI	2.289e-53	61.71	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q7XV20_ORYSJ	1.593e-38	48.84	OSJNBa0064H22.10 protein OS=Oryza sativa subsp. ja...	[more]
Q0JCV1_ORYSJ	1.593e-38	48.84	Os04g0448300 protein OS=Oryza sativa subsp. japoni...	[more]
Q01J77_ORYSA	1.593e-38	48.84	H0818E04.1 protein OS=Oryza sativa GN=OSIGBa0152K1...	[more]

BLAST of GT623127 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
NEP1_NEPGR	1.612e-35	47.62	Aspartic proteinase nepenthesin-1 OS=Nepenthes gra...	[more]
NEP2_NEPGR	1.726e-29	39.64	Aspartic proteinase nepenthesin-2 OS=Nepenthes gra...	[more]

BLAST of GT623127 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G03200.1	1.284e-57	62.29	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT1G25510.1	1.483e-21	36.47	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT3G61820.1	9.614e-21	36.26	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT3G18490.1	1.535e-18	32.35	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT1G01300.1	9.949e-18	32.14	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT5G33340.1	3.781e-17	30.41	\| Symbols: CDR1 \| Eukaryotic aspartyl protease fam...	[more]
AT3G20015.1	3.781e-17	33.33	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT3G25700.1	7.130e-16	29.21	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT1G09750.1	2.996e-14	28.16	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT3G52500.1	1.942e-13	30.17	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Peptidase A1	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 9..176 score: 4.6
IPR001461	Peptidase A1	PFAM	PF00026	Asp	coord: 11..171 score: 9.3
IPR001969	Peptidase aspartic, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 54..65 score
IPR009007	Peptidase aspartic, catalytic	GENE3D	2.40.70.10	no description	coord: 7..178 score: 1.2
IPR021109	Peptidase aspartic	SUPERFAMILY	50630	Acid proteases	coord: 3..176 score: 8.9
None	No IPR available	PANTHER	PTHR13683:SF98	CHLOROPLAST NUCLEIOD DNA-BINDING-RELATED	coord: 9..176 score: 4.6
None	No IPR available	SEG	seg	seg	coord: 47..63 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT623127 ID=GT623127; Name=GT623127; organism=Lens culinaris; type=EST; length=680bp
CGCAAATGTTCATGTCACAAAACATGTCACAACACCACTTATTACAAACC
CATCACAACCCTCTTTTTATTATCTTTCACTCGAAGGAATCTCGGTGGGT
GACACAAGATTATCCATTGAGAAATCAACTTTTGAAGTTAGTGACGATGG
AAGTGGTGGTATGATTATTGATTCTGGCACAACCATTACTTACATTGAAG
AAAATGCTTTTGGCTCATTGAAGAAAGAGTTCATTTCTCAGACGAATTTA
CCGGTCGATGATTCGGGTTCGACCGGATTAGATGTTTGTTTTAGTCTTCC
ATCAGGGAAAACACAAGTCGAAATTCCAAAACTGGTTTTTCATTTTAAAG
GTGGTGATTTGGAGTTGGCTGGTGAAAATTATATGATTGGTGATTCGAGT
TTAGGCGTGGCTTGTTTAGCTATGGGAGCTTCTCAAGGAATGTCTATCTT
TGGTAATGTTCAGCAACAGAATATTTTGGTGAATCATGATCTTGAGAAAG
AAACTATTTCTTTTGTTCCTACTCAGTGTAATAAATTGTGACGAGTTTGT
GTTATATGGTTCTTATGGACTCATCTAAATCTCTTTGTTTCTTTGATTGT
TTTCTATTATGTTGCCTTAGAACTAGTGTTGTTTTTATGATTGAATTTCT
TAGTTGGTTTAATAATATGATGATGGTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR001461	Aspartic_peptidase_A1
IPR001969	Aspartic_peptidase_AS
IPR009007	Peptidase aspartic, catalytic
IPR021109	Peptidase_aspartic_dom_sf

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