GT626003

EST Overview


Name	GT626003
Unique Name	GT626003
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	454

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT626003 vs. TrEMBL
Match: Q8L949_ARATH (Plastid protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.345e-29
Identity = 62/66 (93.94%), Postives = 64/66 (96.97%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQ+RVEPQPQR QDRPRYNDRTRY RR+ENTR
Sbjct:  154 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRXQDRPRYNDRTRYSRRRENTR 219

[Close]

BLAST of GT626003 vs. TrEMBL
Match: Q7DLI5_ARATH (Plastid protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.757e-29
Identity = 62/66 (93.94%), Postives = 64/66 (96.97%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQ+RVEPQPQR QDRPRYNDRTRY RR+ENTR
Sbjct:  133 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRAQDRPRYNDRTRYSRRRENTR 198

[Close]

BLAST of GT626003 vs. TrEMBL
Match: O24657_ARATH (DAL1 protein OS=Arabidopsis thaliana GN=dal1 PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.757e-29
Identity = 62/66 (93.94%), Postives = 64/66 (96.97%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQ+RVEPQPQR QDRPRYNDRTRY RR+ENTR
Sbjct:  154 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRAQDRPRYNDRTRYSRRRENTR 219

[Close]

BLAST of GT626003 vs. TrEMBL
Match: O22793_ARATH (At2g33430/F4P9.20 OS=Arabidopsis thaliana GN=At2g33430 PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.757e-29
Identity = 62/66 (93.94%), Postives = 64/66 (96.97%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQ+RVEPQPQR QDRPRYNDRTRY RR+ENTR
Sbjct:  154 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRAQDRPRYNDRTRYSRRRENTR 219

[Close]

BLAST of GT626003 vs. TrEMBL
Match: D7LGA3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_482286 PE=4 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.757e-29
Identity = 62/66 (93.94%), Postives = 64/66 (96.97%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQ+RVEPQPQR QDRPRYNDRTRY RR+ENTR
Sbjct:  154 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRAQDRPRYNDRTRYSRRRENTR 219

[Close]

BLAST of GT626003 vs. TrEMBL
Match: B9T3R2_RICCO (DAG protein, chloroplast, putative OS=Ricinus communis GN=RCOM_0038710 PE=4 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 6.676e-29
Identity = 61/66 (92.42%), Postives = 63/66 (95.45%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQ+RVEPQPQR  DRPRYNDRTRYVRR+EN R
Sbjct:  183 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRANDRPRYNDRTRYVRRRENMR 248

[Close]

BLAST of GT626003 vs. TrEMBL
Match: B9HSR6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_725889 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 9.640e-28
Identity = 60/66 (90.91%), Postives = 61/66 (92.42%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPE KDYGAELFVNGEIVQR PERQKRVEPQPQR  DRPRYNDRTRYVRR+EN R
Sbjct:   80 GVLFVLPDSYVDPEYKDYGAELFVNGEIVQRPPERQKRVEPQPQRANDRPRYNDRTRYVRRRENMR 145

[Close]

BLAST of GT626003 vs. TrEMBL
Match: A9PHD1_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 9.640e-28
Identity = 60/66 (90.91%), Postives = 61/66 (92.42%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPE KDYGAELFVNGEIVQR PERQKRVEPQPQR  DRPRYNDRTRYVRR+EN R
Sbjct:  176 GVLFVLPDSYVDPEYKDYGAELFVNGEIVQRPPERQKRVEPQPQRANDRPRYNDRTRYVRRRENMR 241

[Close]

BLAST of GT626003 vs. TrEMBL
Match: D7U342_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_5.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00028387001 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.644e-27
Identity = 58/66 (87.88%), Postives = 63/66 (95.45%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPE+Q+RVEPQPQ  +DRP+YNDRTRYVRR+EN R
Sbjct:   80 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPEQQRRVEPQPQTGRDRPKYNDRTRYVRRRENMR 145

[Close]

BLAST of GT626003 vs. TrEMBL
Match: D7SZL0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_49.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027872001 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.644e-27
Identity = 59/66 (89.39%), Postives = 62/66 (93.94%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPE KDYGAELFVNGEIVQRSPERQ+RVEP PQR QDRPRYND+TRYVRR+EN R
Sbjct:   51 GVLFVLPDSYVDPEYKDYGAELFVNGEIVQRSPERQRRVEPAPQRAQDRPRYNDKTRYVRRRENMR 116

[Close]

BLAST of GT626003 vs. SwissProt
Match: UMP1_ARATH (Uncharacterized protein At3g15000, mitochondrial OS=Arabidopsis thaliana GN=At3g15000 PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.959e-8
Identity = 32/70 (45.71%), Postives = 42/70 (60.00%), Query Frame = 3

Query:    6 VLFVLPDSYVDPENKDYGAELFVNGEIVQRSPE------RQKRVEPQPQRHQDRPRYNDRTR-YVRRKEN 194
            V +VLPDSY+D  NKDYG E F++G+ V   P+      R      +  R  DRPR NDR+R + RR+EN
Sbjct:  167 VRWVLPDSYLDVRNKDYGGEPFIDGKAVPYDPKYHEEWIRNNARANERNRRNDRPRNNDRSRNFERRREN 236

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT2G33430.1 (| Symbols: DAL1, DAL | differentiation and greening-like 1 | chr2:14162732-14164729 FORWARD LENGTH=219)

HSP 1 Score: 132.109 bits (331), Expect = 1.257e-31
Identity = 62/66 (93.94%), Postives = 64/66 (96.97%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKRVEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQ+RVEPQPQR QDRPRYNDRTRY RR+ENTR
Sbjct:  154 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRAQDRPRYNDRTRYSRRRENTR 219

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT1G32580.1 (| Symbols: | plastid developmental protein DAG, putative | chr1:11784108-11785430 FORWARD LENGTH=229)

HSP 1 Score: 103.99 bits (258), Expect = 3.666e-23
Identity = 49/67 (73.13%), Postives = 58/67 (86.57%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKR-VEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLF+LPDSYVD ENKDYGAELFVNGEIVQR PERQ++ +E   QR  D+P+Y+D+TRYVRR+EN R
Sbjct:  163 GVLFILPDSYVDQENKDYGAELFVNGEIVQRPPERQRKIIELTTQRTNDKPKYHDKTRYVRRRENMR 229

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT2G35240.1 (| Symbols: | plastid developmental protein DAG, putative | chr2:14845099-14846262 REVERSE LENGTH=232)

HSP 1 Score: 97.8265 bits (242), Expect = 2.627e-21
Identity = 49/67 (73.13%), Postives = 55/67 (82.09%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQKR-VEPQPQRHQDRPRYNDRTRYVRRKENTR 200
            GVLFVLPDSYVDPE KDYGAELFVNGE+V R PERQ+R VE   QR  D+P+Y+DR R VRR+EN R
Sbjct:  166 GVLFVLPDSYVDPEFKDYGAELFVNGEVVPRPPERQRRMVELTNQRGSDKPKYHDRIRNVRRRENMR 232

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT3G15000.1 (| Symbols: | cobalt ion binding | chr3:5050321-5052121 FORWARD LENGTH=395)

HSP 1 Score: 58.151 bits (139), Expect = 2.307e-9
Identity = 32/70 (45.71%), Postives = 42/70 (60.00%), Query Frame = 3

Query:    6 VLFVLPDSYVDPENKDYGAELFVNGEIVQRSPE------RQKRVEPQPQRHQDRPRYNDRTR-YVRRKEN 194
            V +VLPDSY+D  NKDYG E F++G+ V   P+      R      +  R  DRPR NDR+R + RR+EN
Sbjct:  167 VRWVLPDSYLDVRNKDYGGEPFIDGKAVPYDPKYHEEWIRNNARANERNRRNDRPRNNDRSRNFERRREN 236

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT3G06790.2 (| Symbols: | plastid developmental protein DAG, putative | chr3:2144564-2145743 REVERSE LENGTH=244)

HSP 1 Score: 57.7658 bits (138), Expect = 3.013e-9
Identity = 32/68 (47.06%), Postives = 43/68 (63.24%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPER---QKRVEPQPQRHQDRPRYNDR--TRYVRRKE 191
            GVL+VLPDSY+D  NKDYG +L+V G+++ R   R   Q+   P+P     RPRY+ R  T  V R+E
Sbjct:  163 GVLWVLPDSYLDVPNKDYGGDLYVEGKVIPRPQYRFTEQRHTRPRP-----RPRYDRRRETMQVERRE 225

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT3G06790.1 (| Symbols: | plastid developmental protein DAG, putative | chr3:2144564-2145743 REVERSE LENGTH=244)

HSP 1 Score: 57.7658 bits (138), Expect = 3.013e-9
Identity = 32/68 (47.06%), Postives = 43/68 (63.24%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPER---QKRVEPQPQRHQDRPRYNDR--TRYVRRKE 191
            GVL+VLPDSY+D  NKDYG +L+V G+++ R   R   Q+   P+P     RPRY+ R  T  V R+E
Sbjct:  163 GVLWVLPDSYLDVPNKDYGGDLYVEGKVIPRPQYRFTEQRHTRPRP-----RPRYDRRRETMQVERRE 225

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT4G20020.2 (| Symbols: | unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G44780.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). | chr4:10844360-10846085 REVERSE LENGTH=406)

HSP 1 Score: 53.9138 bits (128), Expect = 4.351e-8
Identity = 23/45 (51.11%), Postives = 32/45 (71.11%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQK-RVEPQPQ 134
            GV+F+LPDSY+DP+NK+YG + + NG I  R P  Q  R  P+P+
Sbjct:  159 GVVFILPDSYIDPQNKEYGGDKYENGVITHRPPPIQSGRARPRPR 203

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT4G20020.1 (| Symbols: | unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G44780.1); Has 28928 Blast hits to 16023 proteins in 1033 species: Archae - 4; Bacteria - 4155; Metazoa - 15463; Fungi - 2938; Plants - 3091; Viruses - 205; Other Eukaryotes - 3072 (source: NCBI BLink). | chr4:10844433-10846085 REVERSE LENGTH=419)

HSP 1 Score: 53.9138 bits (128), Expect = 4.351e-8
Identity = 23/45 (51.11%), Postives = 32/45 (71.11%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIVQRSPERQK-RVEPQPQ 134
            GV+F+LPDSY+DP+NK+YG + + NG I  R P  Q  R  P+P+
Sbjct:  159 GVVFILPDSYIDPQNKEYGGDKYENGVITHRPPPIQSGRARPRPR 203

[Close]

BLAST of GT626003 vs. TAIR peptide
Match: AT1G11430.1 (| Symbols: | plastid developmental protein DAG, putative | chr1:3847273-3848938 FORWARD LENGTH=232)

HSP 1 Score: 50.0618 bits (118), Expect = 6.283e-7
Identity = 22/52 (42.31%), Postives = 36/52 (69.23%), Query Frame = 3

Query:    3 GVLFVLPDSYVDPENKDYGAELFVNGEIV-----QRSPERQKRVEPQPQRHQ 143
            GVL+VLPDSY+D +NKDYG + ++NGEI+        P+++   + Q +R++
Sbjct:  157 GVLWVLPDSYIDVKNKDYGGDKYINGEIIPCTYPTYQPKQRNNTKYQSKRYE 208

The following BLAST results are available for this feature:

BLAST of GT626003 vs. TrEMBL
BLAST of GT626003 vs. SwissProt
BLAST of GT626003 vs. TAIR peptide

BLAST of GT626003 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8L949_ARATH	1.345e-29	93.94	Plastid protein OS=Arabidopsis thaliana PE=2 SV=1	[more]
Q7DLI5_ARATH	1.757e-29	93.94	Plastid protein OS=Arabidopsis thaliana PE=2 SV=1	[more]
O24657_ARATH	1.757e-29	93.94	DAL1 protein OS=Arabidopsis thaliana GN=dal1 PE=2 ...	[more]
O22793_ARATH	1.757e-29	93.94	At2g33430/F4P9.20 OS=Arabidopsis thaliana GN=At2g3...	[more]
D7LGA3_ARALY	1.757e-29	93.94	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9T3R2_RICCO	6.676e-29	92.42	DAG protein, chloroplast, putative OS=Ricinus comm...	[more]
B9HSR6_POPTR	9.640e-28	90.91	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PHD1_POPTR	9.640e-28	90.91	Putative uncharacterized protein OS=Populus tricho...	[more]
D7U342_VITVI	1.644e-27	87.88	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SZL0_VITVI	1.644e-27	89.39	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of GT626003 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
UMP1_ARATH	1.959e-8	45.71	Uncharacterized protein At3g15000, mitochondrial O...	[more]

BLAST of GT626003 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 9


Match Name	E-value	Identity	Description
AT2G33430.1	1.257e-31	93.94	\| Symbols: DAL1, DAL \| differentiation and greenin...	[more]
AT1G32580.1	3.666e-23	73.13	\| Symbols: \| plastid developmental protein DAG, p...	[more]
AT2G35240.1	2.627e-21	73.13	\| Symbols: \| plastid developmental protein DAG, p...	[more]
AT3G15000.1	2.307e-9	45.71	\| Symbols: \| cobalt ion binding \| chr3:5050321-50...	[more]
AT3G06790.2	3.013e-9	47.06	\| Symbols: \| plastid developmental protein DAG, p...	[more]
AT3G06790.1	3.013e-9	47.06	\| Symbols: \| plastid developmental protein DAG, p...	[more]
AT4G20020.2	4.351e-8	51.11	\| Symbols: \| unknown protein; BEST Arabidopsis th...	[more]
AT4G20020.1	4.351e-8	51.11	\| Symbols: \| unknown protein; BEST Arabidopsis th...	[more]
AT1G11430.1	6.283e-7	42.31	\| Symbols: \| plastid developmental protein DAG, p...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 26..37 score
None	No IPR available	SEG	seg	seg	coord: 43..53 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT626003 ID=GT626003; Name=GT626003; organism=Lens culinaris; type=EST; length=454bp
CTGGAGTTCTGTTTGTTCTCCCAGATTCATATGTTGATCCCGAAAACAAG
GACTACGGTGCTGAGTTATTTGTGAATGGAGAAATTGTCCAAAGGTCACC
CGAAAGACAGAAAAGGGTGGAGCCACAGCCACAGAGACATCAAGACAGAC
CGAGATACAATGATAGAACAAGGTATGTCCGACGCAAAGAAAACACAAGA
TGAAGTAGATTTCGATAACAAGAAGAGGACAGGGATATAGAGGGCAAGAG
AGGGACATGCTCTGAAAGAGTGAAGGTTGAACTGTTATAGTCACAGCCTT
TGCATGCAGAAATCTTATTTGTTCGATGTTCTATTGGTAAATAGATATGT
TTGTAGTTGAACCAATATGAATATAAACTTTATTATGCTATCAATGCCTT
GTCCTTAATCACTTCTCTTACAAACTGTCAACATAAAAATATGCACGTAT
GAAT

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