GT627251

EST Overview


Name	GT627251
Unique Name	GT627251
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	789

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT627251 vs. TrEMBL
Match: B9SY85_RICCO (GrpE protein homolog OS=Ricinus communis GN=RCOM_0845250 PE=3 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 2.015e-52
Identity = 104/129 (80.62%), Postives = 113/129 (87.60%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDS 387
            E LLPVLDNFERAKAQIK+ETEGEEK+NNSYQSIYKQF+EIL SLGV PVET+GNPFDP+LHEAIMREDS EFE+GII+QEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQ     E  T G + +  S
Sbjct:  221 ENLLPVLDNFERAKAQIKLETEGEEKINNSYQSIYKQFVEILGSLGVVPVETIGNPFDPLLHEAIMREDSTEFEEGIILQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQAESSAEVETAGETSQEGS 349

[Close]

BLAST of GT627251 vs. TrEMBL
Match: C5XXB8_SORBI (GrpE protein homolog OS=Sorghum bicolor GN=Sb04g025770 PE=3 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 1.765e-48
Identity = 103/137 (75.18%), Postives = 115/137 (83.94%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGP---AKPEQEVPLEEQVTNGISEESDSNAKTE 402
            E+ LPVLDNFERAK QIKVETEGEEK+NNSYQSIYKQFIEILNSLGVE VETVG PFDPMLHEAIMREDS E+E+GII+QEFRKGFKLG+RLLRP+MVKVSAGPGP   A P  EV  ++  T  + E+S +  K E
Sbjct:  189 ESFLPVLDNFERAKMQIKVETEGEEKINNSYQSIYKQFIEILNSLGVEDVETVGKPFDPMLHEAIMREDSSEYEEGIILQEFRKGFKLGERLLRPAMVKVSAGPGPEVSAGPGPEVSRDDDPT--VVEDSVAPQKIE 323

[Close]

BLAST of GT627251 vs. TrEMBL
Match: B6TXQ6_MAIZE (GrpE protein homolog OS=Zea mays PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 5.135e-48
Identity = 95/108 (87.96%), Postives = 102/108 (94.44%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAK 324
            E+ LPVLDNFERAK QIKVETEGEEK+NNSYQSIYKQFIEILNSLGVE VETVG PFDPMLHEAIMRE+S EFE+GII+QEFRKGFKLG+RLLRP+MVKVSAGPGP K
Sbjct:  189 ESFLPVLDNFERAKVQIKVETEGEEKINNSYQSIYKQFIEILNSLGVEDVETVGKPFDPMLHEAIMREESSEFEEGIILQEFRKGFKLGERLLRPAMVKVSAGPGPEK 296

[Close]

BLAST of GT627251 vs. TrEMBL
Match: B9I664_POPTR (GrpE protein homolog OS=Populus trichocarpa GN=POPTRDRAFT_571297 PE=3 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 6.707e-48
Identity = 97/127 (76.38%), Postives = 108/127 (85.04%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQ--EVPLEEQVTNGISE 375
            E LL VLDNFERAK QIK  TEGEEK+NNSYQ+IYKQF+EIL SLGV PVET+G PFDPMLHEAIMREDSD FE+G +++E+RKGFKLGDRLLRPSMVKVSAGPGP KPEQ  E   E + T+G SE
Sbjct:  207 ENLLSVLDNFERAKTQIKTATEGEEKINNSYQNIYKQFMEILVSLGVVPVETIGKPFDPMLHEAIMREDSDAFEEGTVLEEYRKGFKLGDRLLRPSMVKVSAGPGPVKPEQVEESQEEAEATSGTSE 333

[Close]

BLAST of GT627251 vs. TrEMBL
Match: D7LX36_ARALY (EMB1241 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_909770 PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 1.652e-46
Identity = 97/131 (74.05%), Postives = 110/131 (83.97%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDSNA 393
            E LL VLDNFERAK+QIKVETEGEEKV NSYQSIYKQF+EIL SLGV  VETVG  FDPMLHEAIMREDS E+E+GI+++E+RKGF LG+RLLRPSMVKVSAGPGP KP +E  +EE    G SEE  S++
Sbjct:  197 ENLLAVLDNFERAKSQIKVETEGEEKVTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPEKP-REAEVEETTAQGSSEEEASSS 326

[Close]

BLAST of GT627251 vs. TrEMBL
Match: Q6K635_ORYSJ (GrpE protein homolog OS=Oryza sativa subsp. japonica GN=OJ1004_A05.20 PE=3 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 2.158e-46
Identity = 97/139 (69.78%), Postives = 114/139 (82.01%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVP--LEEQVT-NGISEESDSNAKTESA 408
            E+LLPVLDNFERAK QIKVETE E K+N+SYQSIYKQFI+ILNSLGVE VETVG PFDPMLHEAIMRE+S E+E+G+I+QEFRKGFKLG+RLLRP+MVKVSAGPGP KP  + P  +E+ V    + E  D     ++A
Sbjct:  193 ESLLPVLDNFERAKTQIKVETEQETKINDSYQSIYKQFIDILNSLGVEDVETVGKPFDPMLHEAIMREESVEYEEGVILQEFRKGFKLGERLLRPAMVKVSAGPGPEKPVYDDPAMVEDSVAPQKVKEAEDDGFDDDNA 331

[Close]

BLAST of GT627251 vs. TrEMBL
Match: B9F116_ORYSJ (GrpE protein homolog OS=Oryza sativa subsp. japonica GN=OsJ_07499 PE=3 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 2.158e-46
Identity = 97/139 (69.78%), Postives = 114/139 (82.01%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVP--LEEQVT-NGISEESDSNAKTESA 408
            E+LLPVLDNFERAK QIKVETE E K+N+SYQSIYKQFI+ILNSLGVE VETVG PFDPMLHEAIMRE+S E+E+G+I+QEFRKGFKLG+RLLRP+MVKVSAGPGP KP  + P  +E+ V    + E  D     ++A
Sbjct:  193 ESLLPVLDNFERAKTQIKVETEQETKINDSYQSIYKQFIDILNSLGVEDVETVGKPFDPMLHEAIMREESVEYEEGVILQEFRKGFKLGERLLRPAMVKVSAGPGPEKPVYDDPAMVEDSVAPQKVKEAEDDGFDDDNA 331

[Close]

BLAST of GT627251 vs. TrEMBL
Match: B8AFE0_ORYSI (GrpE protein homolog OS=Oryza sativa subsp. indica GN=OsI_08039 PE=3 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 2.158e-46
Identity = 97/139 (69.78%), Postives = 114/139 (82.01%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVP--LEEQVT-NGISEESDSNAKTESA 408
            E+LLPVLDNFERAK QIKVETE E K+N+SYQSIYKQFI+ILNSLGVE VETVG PFDPMLHEAIMRE+S E+E+G+I+QEFRKGFKLG+RLLRP+MVKVSAGPGP KP  + P  +E+ V    + E  D     ++A
Sbjct:  193 ESLLPVLDNFERAKTQIKVETEQETKINDSYQSIYKQFIDILNSLGVEDVETVGKPFDPMLHEAIMREESVEYEEGVILQEFRKGFKLGERLLRPAMVKVSAGPGPEKPVYDDPAMVEDSVAPQKVKEAEDDGFDDDNA 331

[Close]

BLAST of GT627251 vs. TrEMBL
Match: B4FLR0_MAIZE (GrpE protein homolog OS=Zea mays PE=2 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 2.818e-46
Identity = 93/114 (81.58%), Postives = 102/114 (89.47%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVP 342
            E+ LPVLDNFERAK QIKVETEGEEK+NNSYQSI KQFIEILNSLGVE VETVG PFDPMLHEAIMRE+S E+E+GII+QEFRKGFKLG+RLLRP+MVKVSAGPGP     + P
Sbjct:  189 ESFLPVLDNFERAKMQIKVETEGEEKINNSYQSINKQFIEILNSLGVEDVETVGKPFDPMLHEAIMREESSEYEEGIILQEFRKGFKLGERLLRPAMVKVSAGPGPENSGDDDP 302

[Close]

BLAST of GT627251 vs. TrEMBL
Match: B6TLM3_MAIZE (GrpE protein homolog OS=Zea mays PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 1.398e-45
Identity = 92/114 (80.70%), Postives = 101/114 (88.60%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVP 342
            E+ LPVLDNFERAK QIKVETEGEEK+NNSYQSI KQFIEILNSL VE VETVG PFDPMLHEAIMRE+S E+E+GII+QEFRKGFKLG+RLLRP+MVKVSAGPGP     + P
Sbjct:  189 ESFLPVLDNFERAKMQIKVETEGEEKINNSYQSINKQFIEILNSLSVEDVETVGKPFDPMLHEAIMREESSEYEEGIILQEFRKGFKLGERLLRPAMVKVSAGPGPENSGDDDP 302

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROMT (Protein grpE OS=Prochlorococcus marinus (strain NATL2A) GN=grpE PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.717e-29
Identity = 60/133 (45.11%), Postives = 89/133 (66.92%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDSNAKTES 405
            +LP++DNFERA+ Q+  E E  + ++ SYQ +YKQ +E+L +LGV P+  V   FDP LHEA+MRE SDE  + I+I+E ++G+ L  R+LR ++VKVS GPGP    +E+P +      +SE  D + K E+
Sbjct:  127 ILPIVDNFERARQQLNPEGEEAQALHRSYQGLYKQLVEVLKNLGVAPMRVVDQAFDPSLHEAVMREPSDEKAEDIVIEELQRGYHLNGRVLRHALVKVSMGPGPKVINEEIPDQSASNQELSESVDGSTKDEN 259

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROM1 (Protein grpE OS=Prochlorococcus marinus (strain NATL1A) GN=grpE PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 2.242e-29
Identity = 60/133 (45.11%), Postives = 88/133 (66.17%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDSNAKTES 405
            +LP++DNFERA+ Q+  E E  + ++ SYQ +YKQ +E+L +LGV P+  V   FDP LHEA+MRE SDE  + I+I+E ++G+ L  R+LR ++VKVS GPGP    +E+P +      +SE  D   K E+
Sbjct:  127 ILPIVDNFERARQQLNPEGEEAQALHRSYQGLYKQLVEVLKNLGVAPMRVVDQAFDPSLHEAVMREPSDEKAEDIVIEELQRGYHLNGRVLRHALVKVSMGPGPKAVNEEIPDQSASNQELSESVDGPTKDEN 259

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROM0 (Protein grpE OS=Prochlorococcus marinus (strain MIT 9301) GN=grpE PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 2.479e-28
Identity = 61/131 (46.56%), Postives = 90/131 (68.70%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDSNAKT 399
            +LP++DNFERA+ Q+K E+E  + ++ SYQ +YKQ +E+L   GV P+  VG  FDP LHEA++RE S+EFE+  II+E ++G+ L  ++LR ++ KVS GPG  K +QEV  E+    G   + DS+A T
Sbjct:  110 ILPIVDNFERARQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAVLREPSEEFEEDFIIEELQRGYHLEGKVLRHALAKVSMGPGKQKSQQEV--EKDTVEG---DVDSDANT 235

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROM4 (Protein grpE OS=Prochlorococcus marinus (strain MIT 9211) GN=grpE PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 5.523e-28
Identity = 56/133 (42.11%), Postives = 89/133 (66.92%), Query Frame = 1

Query:    4 TLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDSNAKTE 402
            ++LPV+DNF+RA+ Q+  E E  + ++ SYQ +YKQ +++L  LGV P+  VG  FDP LHEA++RE SDE  + II++E ++G+ L  R+LR ++VKVS GPGP    +E   E+ +    + +  S+ K++
Sbjct:  115 SILPVVDNFDRARQQLNPEGEEAQALHKSYQGLYKQLVDVLKQLGVAPMRVVGQTFDPSLHEAVLREPSDELAEDIIVEELQRGYHLNGRVLRHALVKVSMGPGPKDDGEETITEQSLEGDNTTDQQSSEKSD 247

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROMS (Protein grpE OS=Prochlorococcus marinus (strain AS9601) GN=grpE PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 9.421e-28
Identity = 57/127 (44.88%), Postives = 89/127 (70.08%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDS 387
            +LP++DNFERA+ Q+K E+E  + ++ SYQ +YKQ +E+L   GV P+  VG  FDP LHEA++RE S++FE+  II+E ++G+ L  ++LR ++VKVS GPG    ++EV  ++   +  SEE+ S
Sbjct:  110 ILPIVDNFERARQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPSLHEAVLREPSEKFEEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQEEVEKDKVEGDIDSEENTS 236

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROMP (Protein grpE OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) GN=grpE PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.099e-27
Identity = 60/130 (46.15%), Postives = 90/130 (69.23%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPG---PAKPEQEVPLEEQV--TNGISEES 381
            +LP++DNFERA+ Q+K E+E  + ++ SYQ +YKQ +E+L   GV P+  V   FDP LHEA++RE S EF + III+E ++G+ L  ++LR ++VKVS GPG     +PE++  +EE +   N ISEE+
Sbjct:  110 ILPIVDNFERARQQLKPESEEAQTLHRSYQGLYKQLVEVLKQQGVSPMRVVAQQFDPKLHEAVLREPSQEFNEDIIIEELQRGYHLEGKVLRHALVKVSMGPGQQNSQEPEEKDKVEEDIDSENPISEEN 239

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_SYNPW (Protein grpE OS=Synechococcus sp. (strain WH7803) GN=grpE PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 7.975e-27
Identity = 55/127 (43.31%), Postives = 83/127 (65.35%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDS 387
            +LPV+DNFERA+ Q+  + E  + ++ SYQ +YKQ +++L  LGV P+  VG  FDP LHEA++RE SD   + ++I+E ++G+ L  ++LR +MVKVS GPGP       P E    +  +E+S S
Sbjct:  109 ILPVVDNFERARQQLDPQGEEAQALHRSYQGLYKQLVDVLKQLGVAPMRVVGQEFDPTLHEAVLREPSDAHPEDVVIEELQRGYHLNGKVLRHAMVKVSMGPGPQDGASSQPAEAPAADAPAEDSGS 235

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROM2 (Protein grpE OS=Prochlorococcus marinus (strain MIT 9215) GN=grpE PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 7.975e-27
Identity = 56/129 (43.41%), Postives = 88/129 (68.22%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEV---PLEEQVTNGISEESD 384
            +LP++DNFERA+ Q++ E+E  + ++ SYQ +YKQ +E+L   GV P+  VG  FDP LHEA++RE S+E ++  II+E ++G+ L  ++LR ++VKVS GPG    +QEV    +EE V + ++   D
Sbjct:  110 ILPIVDNFERARQQLQPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAVLREPSEESDEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQQEVEKDTVEEDVNSEVNTSED 238

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_THEEB (Protein grpE OS=Thermosynechococcus elongatus (strain BP-1) GN=grpE PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.777e-26
Identity = 53/102 (51.96%), Postives = 77/102 (75.49%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGP 312
            LLPV+D+FE A+  I+ ETE EEK++ SYQ +YKQ +E L  +GV  ++  G PFDP LHEA++RE ++E  +G +I+E ++G+ LGDR+LR +MVKV+A P
Sbjct:  134 LLPVVDSFELARTHIQTETEAEEKIHRSYQGVYKQLVECLKRIGVSAMQAKGKPFDPNLHEAVLREATNEHPEGTVIEELKRGYMLGDRVLRHAMVKVAAPP 235

[Close]

BLAST of GT627251 vs. SwissProt
Match: GRPE_PROM9 (Protein grpE OS=Prochlorococcus marinus (strain MIT 9312) GN=grpE PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.777e-26
Identity = 55/127 (43.31%), Postives = 88/127 (69.29%), Query Frame = 1

Query:    7 LLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDS 387
            +LP++DNFERA+ Q+K E+E  + ++ SYQ +YKQ +E+L   GV P+  VG  FDP LHEA++RE S+EF++ +I++E ++G+ L  ++LR ++VKVS G G    ++EV  +    +  SEE+ S
Sbjct:  110 ILPIVDNFERARQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAVLREPSEEFKEDLIVEELQRGYHLEGKVLRHALVKVSMGHGQQNSQEEVEKDTVEEDIDSEENTS 236

[Close]

BLAST of GT627251 vs. TAIR peptide
Match: AT5G17710.2 (| Symbols: EMB1241 | Co-chaperone GrpE family protein | chr5:5839560-5841639 REVERSE LENGTH=326)

HSP 1 Score: 186.808 bits (473), Expect = 1.176e-47
Identity = 96/131 (73.28%), Postives = 108/131 (82.44%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDSNA 393
            E LL VLDNFERAK+QIKVETEGEEKV NSYQSIYKQF+EIL SLGV  VETVG  FDPMLHEAIMREDS E+E+GI+++E+RKGF LG+RLLRPSMVKVSAGPGP KP  E   EE    G +EE  S++
Sbjct:  197 ENLLAVLDNFERAKSQIKVETEGEEKVTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPEKP-LEAEGEEATAQGSAEEESSSS 326

[Close]

BLAST of GT627251 vs. TAIR peptide
Match: AT5G17710.1 (| Symbols: EMB1241 | Co-chaperone GrpE family protein | chr5:5839560-5841639 REVERSE LENGTH=324)

HSP 1 Score: 186.808 bits (473), Expect = 1.176e-47
Identity = 96/131 (73.28%), Postives = 108/131 (82.44%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGPGPAKPEQEVPLEEQVTNGISEESDSNA 393
            E LL VLDNFERAK+QIKVETEGEEKV NSYQSIYKQF+EIL SLGV  VETVG  FDPMLHEAIMREDS E+E+GI+++E+RKGF LG+RLLRPSMVKVSAGPGP KP  E   EE    G +EE  S++
Sbjct:  195 ENLLAVLDNFERAKSQIKVETEGEEKVTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPEKP-LEAEGEEATAQGSAEEESSSS 324

[Close]

BLAST of GT627251 vs. TAIR peptide
Match: AT1G36390.2 (| Symbols: | Co-chaperone GrpE family protein | chr1:13701811-13703524 REVERSE LENGTH=279)

HSP 1 Score: 131.339 bits (329), Expect = 5.865e-31
Identity = 59/104 (56.73%), Postives = 83/104 (79.81%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGP 312
            ++LLP++D+FE+AK Q++V+T+ E+K++ SYQ IY+QF+E+L  L V  + TVG PFDP+LHEAI RE+S+  + GII +E  KGF LGDR+LRP+ VKVS GP
Sbjct:  160 KSLLPIIDSFEKAKLQVRVDTDKEKKIDTSYQGIYRQFVEVLRYLRVSVIATVGKPFDPLLHEAISREESEAVKAGIITEELNKGFVLGDRVLRPAKVKVSLGP 263

[Close]

BLAST of GT627251 vs. TAIR peptide
Match: AT1G36390.1 (| Symbols: | Co-chaperone GrpE family protein | chr1:13701811-13703524 REVERSE LENGTH=279)

HSP 1 Score: 131.339 bits (329), Expect = 5.865e-31
Identity = 59/104 (56.73%), Postives = 83/104 (79.81%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSIYKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAGP 312
            ++LLP++D+FE+AK Q++V+T+ E+K++ SYQ IY+QF+E+L  L V  + TVG PFDP+LHEAI RE+S+  + GII +E  KGF LGDR+LRP+ VKVS GP
Sbjct:  160 KSLLPIIDSFEKAKLQVRVDTDKEKKIDTSYQGIYRQFVEVLRYLRVSVIATVGKPFDPLLHEAISREESEAVKAGIITEELNKGFVLGDRVLRPAKVKVSLGP 263

[Close]

BLAST of GT627251 vs. TAIR peptide
Match: AT4G26780.1 (| Symbols: AR192 | Co-chaperone GrpE family protein | chr4:13485066-13486560 REVERSE LENGTH=327)

HSP 1 Score: 52.373 bits (124), Expect = 3.462e-7
Identity = 33/119 (27.73%), Postives = 53/119 (44.54%), Query Frame = 1

Query:    1 ETLLPVLDNFERAKAQIKVETEGEEKVNNSYQSI----------------YKQFIEILNSLGVEPVETVGNPFDPMLHEAIMREDSDEFEDGIIIQEFRKGFKLGDRLLRPSMVKVSAG 309
            ++LL V DN  RA + +K   E   K++ S  S                  KQ  E+    G+E  + +  PFDP  H A+ +       +G +    + G+ L DR++RP+ V V+ G
Sbjct:  200 KSLLDVADNLGRASSVVK---ESFSKLDTSEDSAGAAPLLKTLLEGVEMTEKQLAEVFKKFGMEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRVIRPAEVGVTQG 315

The following BLAST results are available for this feature:

BLAST of GT627251 vs. TrEMBL
BLAST of GT627251 vs. SwissProt
BLAST of GT627251 vs. TAIR peptide

BLAST of GT627251 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SY85_RICCO	2.015e-52	80.62	GrpE protein homolog OS=Ricinus communis GN=RCOM_0...	[more]
C5XXB8_SORBI	1.765e-48	75.18	GrpE protein homolog OS=Sorghum bicolor GN=Sb04g02...	[more]
B6TXQ6_MAIZE	5.135e-48	87.96	GrpE protein homolog OS=Zea mays PE=2 SV=1	[more]
B9I664_POPTR	6.707e-48	76.38	GrpE protein homolog OS=Populus trichocarpa GN=POP...	[more]
D7LX36_ARALY	1.652e-46	74.05	EMB1241 OS=Arabidopsis lyrata subsp. lyrata GN=ARA...	[more]
Q6K635_ORYSJ	2.158e-46	69.78	GrpE protein homolog OS=Oryza sativa subsp. japoni...	[more]
B9F116_ORYSJ	2.158e-46	69.78	GrpE protein homolog OS=Oryza sativa subsp. japoni...	[more]
B8AFE0_ORYSI	2.158e-46	69.78	GrpE protein homolog OS=Oryza sativa subsp. indica...	[more]
B4FLR0_MAIZE	2.818e-46	81.58	GrpE protein homolog OS=Zea mays PE=2 SV=1	[more]
B6TLM3_MAIZE	1.398e-45	80.70	GrpE protein homolog OS=Zea mays PE=2 SV=1	[more]

BLAST of GT627251 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GRPE_PROMT	1.717e-29	45.11	Protein grpE OS=Prochlorococcus marinus (strain NA...	[more]
GRPE_PROM1	2.242e-29	45.11	Protein grpE OS=Prochlorococcus marinus (strain NA...	[more]
GRPE_PROM0	2.479e-28	46.56	Protein grpE OS=Prochlorococcus marinus (strain MI...	[more]
GRPE_PROM4	5.523e-28	42.11	Protein grpE OS=Prochlorococcus marinus (strain MI...	[more]
GRPE_PROMS	9.421e-28	44.88	Protein grpE OS=Prochlorococcus marinus (strain AS...	[more]
GRPE_PROMP	2.099e-27	46.15	Protein grpE OS=Prochlorococcus marinus subsp. pas...	[more]
GRPE_SYNPW	7.975e-27	43.31	Protein grpE OS=Synechococcus sp. (strain WH7803) ...	[more]
GRPE_PROM2	7.975e-27	43.41	Protein grpE OS=Prochlorococcus marinus (strain MI...	[more]
GRPE_THEEB	1.777e-26	51.96	Protein grpE OS=Thermosynechococcus elongatus (str...	[more]
GRPE_PROM9	1.777e-26	43.31	Protein grpE OS=Prochlorococcus marinus (strain MI...	[more]

BLAST of GT627251 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G17710.2	1.176e-47	73.28	\| Symbols: EMB1241 \| Co-chaperone GrpE family prot...	[more]
AT5G17710.1	1.176e-47	73.28	\| Symbols: EMB1241 \| Co-chaperone GrpE family prot...	[more]
AT1G36390.2	5.865e-31	56.73	\| Symbols: \| Co-chaperone GrpE family protein \| c...	[more]
AT1G36390.1	5.865e-31	56.73	\| Symbols: \| Co-chaperone GrpE family protein \| c...	[more]
AT4G26780.1	3.462e-7	27.73	\| Symbols: AR192 \| Co-chaperone GrpE family protei...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000740	GrpE nucleotide exchange factor	PRINTS	PR00773	GRPEPROTEIN	coord: 54..69 score: 1e-11coord: 81..100 score: 1
IPR000740	GrpE nucleotide exchange factor	PANTHER	PTHR21237	GRPE PROTEIN	coord: 1..112 score: 1.5
IPR000740	GrpE nucleotide exchange factor	PFAM	PF01025	GrpE	coord: 2..101 score: 2.1
IPR000740	GrpE nucleotide exchange factor	PROSITE	PS01071	GRPE	coord: 57..100 score
IPR009012	GrpE nucleotide exchange factor, head	GENE3D	2.30.22.10	no description	coord: 46..103 score: 1.4
IPR009012	GrpE nucleotide exchange factor, head	SUPERFAMILY	51064	Head domain of nucleotide exchange factor GrpE	coord: 47..105 score: 5.5
IPR013805	GrpE nucleotide exchange factor, coiled-coil	GENE3D	3.90.20.20	no description	coord: 2..43 score: 1.4
None	No IPR available	PANTHER	PTHR21237:SF4	GRPE PROTEIN (HEAT SHOCK PROTEIN) (CGE1)	coord: 1..112 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627251 ID=GT627251; Name=GT627251; organism=Lens culinaris; type=EST; length=789bp
GAGACTCTGTTGCCTGTATTGGATAATTTTGAGAGAGCCAAAGCTCAGAT
TAAGGTGGAAACCGAGGGAGAGGAGAAAGTAAACAACAGCTATCAGAGCA
TATATAAGCAGTTCATTGAAATTCTAAACTCACTTGGAGTTGAACCAGTG
GAGACAGTTGGAAATCCCTTTGATCCAATGCTACATGAAGCAATTATGCG
CGAGGATTCTGATGAATTTGAAGATGGCATTATAATTCAAGAATTCAGAA
AGGGTTTTAAACTTGGTGATCGCCTCTTACGTCCATCAATGGTGAAGGTA
TCAGCTGGTCCAGGACCTGCAAAGCCGGAACAAGAAGTACCTCTGGAAGA
ACAAGTCACCAATGGAATTTCCGAAGAGAGTGATAGCAACGCAAAAACAG
AGTCTGCTTGAAAGACAAATCTTTGCCTGGATTCAGTTTTGTACAAGTAT
ATCAGTTTAATCTCCAAGAACTTAATGATGGGTGTTACAGTTGCTCAAGA
GATATTCATTTTAAGGGAACTTAGTATTATGAGATTTTGAATTATGATTT
CAGATTTTTATGTCCTTGCACGGTTTTGGTAGAGATGACTAAATTTTCAG
AGGTACCATGTTTCTGAAAGGGCCCCAAGTAAGATGCAATGGATCAAGTG
CTTGGCACTTGGAGTTGGGAGATCAGCGTGAACTTTTAAATTAAGGTTTT
ATAACGACACTGTCTCTTCATCTTATTGCACATTATGTATTATTTAGTTA
AGCTAGAAGCAATTCTAGTTAATATACCTCGTGAGTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000774	adenyl-nucleotide exchange factor activity
GO:0042803	protein homodimerization activity
GO:0051087	protein-folding chaperone binding
GO:0005515	protein binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005739	mitochondrion

Vocabulary: **Biological Process**
Term	Definition
GO:0006457	protein folding
GO:0030150	protein import into mitochondrial matrix

Vocabulary: **INTERPRO**
Term	Definition
IPR000740	GrpE
IPR009012	GrpE_head
IPR013805	GrpE_coiled_coil

Search form