GT627259

EST Overview


Name	GT627259
Unique Name	GT627259
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	781

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT627259 vs. TrEMBL
Match: Q9SC91_MEDTR (Glutamine synthetase I OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 1.475e-105
Identity = 112/124 (90.32%), Postives = 117/124 (94.35%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            SKQDEIVLGDLN+QPGQAWEYCPREALRR SKILKDEFDL MNAGFENEFFLLKS  REG+EEWIQFDSTPYCSSSAFD ASPILRE+TSALHSMGIPVEQLHAEAGKGQFELVLGH +C K+A
Sbjct:   99 SKQDEIVLGDLNLQPGQAWEYCPREALRRVSKILKDEFDLAMNAGFENEFFLLKSAIREGKEEWIQFDSTPYCSSSAFDVASPILREVTSALHSMGIPVEQLHAEAGKGQFELVLGHMVCTKSA 222

HSP 2 Score: 173.711 bits (439), Expect = 1.475e-105
Identity = 87/98 (88.78%), Postives = 89/98 (90.82%), Query Frame = 1

Query:   16 MDLIELRKAEEEVELVNDLETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            MDLIEL KA E VELV+ L+TDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSM MTSFLDG   GSGLGSVGEARLTPDLST RTIPW
Sbjct:    1 MDLIELSKAVENVELVDTLDTDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMVMTSFLDGSPAGSGLGSVGEARLTPDLSTKRTIPW 98

[Close]

BLAST of GT627259 vs. TrEMBL
Match: D7TIU8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033692001 PE=4 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.278e-74
Identity = 87/124 (70.16%), Postives = 110/124 (88.71%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            +KQ+E+VL D++++PG+ WEYCPREALRR SK+LKDEF+LV+NAGFE EF+LLK I REG+EEW+ FDSTPYCS+SAFDAASPI  E+ +AL S+ +PVEQLHAEAGKGQFE+ LGHT+C+ +A
Sbjct:  483 AKQEEMVLADMHLKPGKPWEYCPREALRRISKVLKDEFNLVVNAGFEVEFYLLKRILREGKEEWVPFDSTPYCSTSAFDAASPIFHEVLAALQSLNVPVEQLHAEAGKGQFEIALGHTVCSCSA 606

HSP 2 Score: 110.538 bits (275), Expect = 1.278e-74
Identity = 50/82 (60.98%), Postives = 59/82 (71.95%), Query Frame = 1

Query:   64 NDLETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            N  + D++ VRI+WVD SGQ RCR +P +RFYDVV KNGVGL F  M M+S +DGPA G+ L  VGE RL PDLST   IPW
Sbjct:  401 NSSQNDITLVRIIWVDASGQQRCRVVPARRFYDVVLKNGVGLTFACMGMSSAIDGPADGTNLSGVGETRLVPDLSTKCRIPW 482

[Close]

BLAST of GT627259 vs. TrEMBL
Match: B9S0C8_RICCO (Protein fluG, putative OS=Ricinus communis GN=RCOM_1353330 PE=3 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 5.249e-70
Identity = 81/123 (65.85%), Postives = 103/123 (83.74%), Query Frame = 2

Query:  413 KQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            KQ+E+VL D++++P +AWEYCPREALR+ S +LKDEF+LVMNAGFENEF LL+S +REG+EEWI  DS PYCS++ +DAA+PIL EI +ALHS+ I VEQLH EAGKGQFE+ L +T C  +A
Sbjct:  483 KQEEMVLADMHIKPSEAWEYCPREALRKVSNVLKDEFNLVMNAGFENEFVLLQSASREGKEEWIPIDSAPYCSTAGYDAAAPILHEIVAALHSLNITVEQLHKEAGKGQFEIALEYTTCTISA 605

HSP 2 Score: 110.153 bits (274), Expect = 5.249e-70
Identity = 52/80 (65.00%), Postives = 60/80 (75.00%), Query Frame = 1

Query:   70 LETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            L+  VSFVR++WVD SGQHRCR +P KRF DVV  NG+GL  VSM MTS +DGPA+ + L  VGE RL PDLST R IPW
Sbjct:  402 LDNGVSFVRVIWVDTSGQHRCRVVPIKRFNDVVKTNGLGLTLVSMGMTSAVDGPANETNLTGVGEIRLIPDLSTQRRIPW 481

[Close]

BLAST of GT627259 vs. TrEMBL
Match: B9MTI4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_589509 PE=3 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 6.244e-68
Identity = 81/123 (65.85%), Postives = 102/123 (82.93%), Query Frame = 2

Query:  413 KQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            ++ E+VL D++++PG+ WEYCPREALRR  K+LKDEFDLVM+AGFENEF LLKS++ EG+EEW+  DS PYCS+++FD  SPIL EI  ALHS+ I VEQLHAE+GKGQFEL +GHT C  +A
Sbjct:  475 ERQEMVLADMHLRPGEPWEYCPREALRRVLKVLKDEFDLVMDAGFENEFVLLKSVSWEGKEEWVPIDSAPYCSTASFDLVSPILDEIVGALHSLDITVEQLHAESGKGQFELAMGHTTCPLSA 597

HSP 2 Score: 103.219 bits (256), Expect = 6.244e-68
Identity = 48/80 (60.00%), Postives = 57/80 (71.25%), Query Frame = 1

Query:   70 LETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            LE   S VR++WVD SGQHRCRA+P KRF D+V KNGVGL   SM M+S  D PA  +GL  VGE RL PD++T + IPW
Sbjct:  394 LENSSSLVRVMWVDTSGQHRCRAVPVKRFSDIVRKNGVGLTHASMGMSSAADSPADETGLTGVGEIRLIPDVTTRKKIPW 473

[Close]

BLAST of GT627259 vs. TrEMBL
Match: D7LUF4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_485693 PE=4 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.062e-67
Identity = 80/124 (64.52%), Postives = 104/124 (83.87%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            +KQ+ +VL D++++PG+AWEYCPRE LRR +K+LKDEFDLVMNAGFENEF+LLK++ REG+EE++ F+  PYCS+S+FD ASPI  EI  AL S+ I VEQ HAE+GKGQFE+ LGHT+ + AA
Sbjct:  496 TKQESMVLADMHLKPGEAWEYCPRETLRRVAKVLKDEFDLVMNAGFENEFYLLKNVVREGKEEYVPFEFGPYCSTSSFDVASPIFHEIVPALESLNIEVEQFHAESGKGQFEVSLGHTVASHAA 619

HSP 2 Score: 102.449 bits (254), Expect = 1.062e-67
Identity = 51/79 (64.56%), Postives = 55/79 (69.62%), Query Frame = 1

Query:   73 ETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            E   SFVRI+WVD SGQ RCRA+  +RF   V KNGVGL F SM MTSF DGPA  S L  VGE RL PDLST +TI W
Sbjct:  417 EDSSSFVRIIWVDTSGQQRCRAVQAQRFNKSVKKNGVGLTFASMGMTSFTDGPAEESNLTGVGEIRLVPDLSTKQTIRW 495

[Close]

BLAST of GT627259 vs. TrEMBL
Match: Q9SCP3_ARATH (Nodulin / glutamate-ammonia ligase-like protein OS=Arabidopsis thaliana GN=T4D2.110 PE=1 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 1.385e-67
Identity = 80/124 (64.52%), Postives = 103/124 (83.06%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            +KQ+ +VL D+ ++PG+AW YCPRE LRR +K+LKDEFDLVMNAGFENEF+LLK++ REG+EE++ FD  PYC++S+FDAASPI  +I  AL S+ I VEQ HAE+GKGQFE+ LGHTI + AA
Sbjct:  488 TKQESMVLADMQLKPGEAWGYCPRETLRRVAKVLKDEFDLVMNAGFENEFYLLKNVVREGKEEYMPFDFGPYCATSSFDAASPIFHDIVPALESLNIEVEQFHAESGKGQFEVSLGHTIASHAA 611

HSP 2 Score: 104.76 bits (260), Expect = 1.385e-67
Identity = 52/79 (65.82%), Postives = 56/79 (70.89%), Query Frame = 1

Query:   73 ETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            E   SFVRI+WVD SGQ RCRA+  +RF   V KNGVGL F SM MTSF DGPA  S L  VGE RL PDLST +TIPW
Sbjct:  409 EDSSSFVRIIWVDTSGQQRCRAVQAQRFNRSVKKNGVGLTFASMGMTSFTDGPAEESKLTGVGEIRLVPDLSTKQTIPW 487

[Close]

BLAST of GT627259 vs. TrEMBL
Match: C5WZH7_SORBI (Putative uncharacterized protein Sb01g020230 OS=Sorghum bicolor GN=Sb01g020230 PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 9.614e-63
Identity = 69/124 (55.65%), Postives = 98/124 (79.03%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            S+++E+V+ D+ ++PG+ WEYCPR ALR+ +K+L DEF++ M AGFENEFFL + +  +G E+W+ +D+T YCS+SAFD AS IL+E+ S+L + GI VEQLHAEAGKGQFE+ L + +C  AA
Sbjct:  478 SRREEMVMADMQIRPGEGWEYCPRNALRKVTKVLLDEFNVTMKAGFENEFFLRRKLVSDGVEQWVPYDNTNYCSTSAFDGASSILQEVYSSLKASGIVVEQLHAEAGKGQFEIALKYILCTLAA 601

HSP 2 Score: 106.686 bits (265), Expect = 9.614e-63
Identity = 47/83 (56.63%), Postives = 59/83 (71.08%), Query Frame = 1

Query:   61 VNDLETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            ++ +E DV FVRI+W D SGQ+RCR +P +RFY++    GVGL F +M MTSF DGPA GS L  VGE RL PD+ T+  IPW
Sbjct:  395 LSSVEEDVLFVRIIWSDASGQYRCRVVPARRFYEITRNKGVGLTFAAMGMTSFCDGPADGSNLTGVGEIRLVPDMPTLVRIPW 477

[Close]

BLAST of GT627259 vs. TrEMBL
Match: B8BH89_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_33879 PE=3 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 2.779e-62
Identity = 66/124 (53.23%), Postives = 97/124 (78.23%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            S+++E+V+ +++++PG+AWEYCPR  LR+ +K+L DEF++ M AGFENEFFL K +    +E W+ FD+TPYCS++AFD AS +L+E+ ++L +  I VEQLHAEAGKGQFE+ L + +C  AA
Sbjct:  486 SRREEMVMAEMHIRPGEAWEYCPRNTLRKVTKVLLDEFNVTMMAGFENEFFLRKKVVSGEKELWVPFDNTPYCSTTAFDGASSVLQEVYTSLKAAEIVVEQLHAEAGKGQFEIALKYVLCTLAA 609

HSP 2 Score: 110.923 bits (276), Expect = 2.779e-62
Identity = 47/79 (59.49%), Postives = 58/79 (73.42%), Query Frame = 1

Query:   73 ETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            E DV FVR++W+D SGQHRCR +P  RFY++  K G+GL F SM MTSF DGPA G+ L  VGE RL PD+ST+  +PW
Sbjct:  407 EQDVLFVRVVWIDTSGQHRCRVVPAGRFYEIARKKGIGLTFASMGMTSFTDGPADGTNLTGVGEIRLMPDMSTLLRLPW 485

[Close]

BLAST of GT627259 vs. TrEMBL
Match: Q5MD07_SACOF (Glutamine synthetase (Fragment) OS=Saccharum officinarum GN=GSI PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 4.737e-62
Identity = 68/124 (54.84%), Postives = 98/124 (79.03%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            S+++E+V+ D+ ++PG+ WEYCPR A+R+ +K+L DEF++ M AGFENEFFL + +  +G E+W+ +D+T YCS+SAFD AS IL+E+ S+L + GI VEQLHAEAGKGQFE+ L + +C  AA
Sbjct:  233 SRREEMVMADMQIRPGECWEYCPRNAMRKVTKVLLDEFNVTMKAGFENEFFLRRKLVSDGVEQWVPYDNTNYCSTSAFDGASSILQEVYSSLKASGIVVEQLHAEAGKGQFEIALKYILCTLAA 356

HSP 2 Score: 105.145 bits (261), Expect = 4.737e-62
Identity = 47/83 (56.63%), Postives = 58/83 (69.88%), Query Frame = 1

Query:   61 VNDLETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            ++ +E DV FVRI+W D SGQ+RCR +P  RFY++    GVGL F +M MTSF DGPA GS L  VGE RL PD+ T+  IPW
Sbjct:  150 LSSVEEDVLFVRIIWSDASGQYRCRVVPAGRFYEITRNKGVGLTFAAMGMTSFCDGPADGSNLTGVGEIRLVPDMPTLVRIPW 232

[Close]

BLAST of GT627259 vs. TrEMBL
Match: Q0IX96_ORYSJ (Os10g0456500 protein OS=Oryza sativa subsp. japonica GN=Os10g0456500 PE=2 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 6.160e-62
Identity = 65/124 (52.42%), Postives = 97/124 (78.23%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            S+++E+V+ +++++PG+AWEYCPR  LR+ +K+L DEF++ M AGFENEFFL K +    +E W+ FD+TPYCS++AFD AS +L+E+ ++L +  I VEQLHAE+GKGQFE+ L + +C  AA
Sbjct:  486 SRREEMVMAEMHIRPGEAWEYCPRNTLRKVTKVLLDEFNVTMMAGFENEFFLRKKVVSGEKELWVPFDNTPYCSTTAFDGASSVLQEVYTSLKAAEIVVEQLHAESGKGQFEIALKYVLCTLAA 609

HSP 2 Score: 110.923 bits (276), Expect = 6.160e-62
Identity = 47/79 (59.49%), Postives = 58/79 (73.42%), Query Frame = 1

Query:   73 ETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            E DV FVR++W+D SGQHRCR +P  RFY++  K G+GL F SM MTSF DGPA G+ L  VGE RL PD+ST+  +PW
Sbjct:  407 EQDVLFVRVVWIDTSGQHRCRVVPAGRFYEIARKKGIGLTFASMGMTSFTDGPADGTNLTGVGEIRLMPDMSTLLRLPW 485

[Close]

BLAST of GT627259 vs. SwissProt
Match: GLNA1_DICDI (Type-1 glutamine synthetase 1 OS=Dictyostelium discoideum GN=glnA1 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.657e-14
Identity = 37/106 (34.91%), Postives = 58/106 (54.72%), Query Frame = 2

Query:  464 WEYCPREALRRASKILKDEFDLVMNAGFENEFFLLK------SIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHT 763
            W  CPR +L+RA   LK++F + +   FE EF+L+K      S          + D   + +  + D    IL +IT+AL   G+P+EQL +E+G GQFE+ + +T
Sbjct:  154 WLLCPRNSLQRAIDRLKNKFGISLKGSFEEEFYLIKKGDNNNSSVASLLNSIEKLDHGTFANYHSLDCYGDILEKITNALEEQGLPIEQLLSESGSGQFEITIDYT 259

HSP 2 Score: 27.335 bits (59), Expect = 6.657e-14
Identity = 29/122 (23.77%), Postives = 51/122 (41.80%), Query Frame = 1

Query:   10 ERMDLIELRKAEEEVELVNDLETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTK-NGVGLAFVSMAMTSFLDG-PAHGSGLGSVGEARLTPDLSTIRTIPWYTTSS**KIYINTFFTFSYI 369
            E   ++  ++  E++++       + F+R+ W+D S + R +AI      +   K   V +  V M++  F D          + GEA L P  +T   I  Y  S     +I  F  F Y+
Sbjct:   28 ENQLILNSKQLLEKIKISKKKNPQLKFIRVCWIDISNKIRTKAINIDWILNHEPKLIHVSITNVCMSLLCFEDSVTIEALKSENFGEAFLIPITTTKLNILPYCPS-----HIQIFGEFFYL 144

[Close]

BLAST of GT627259 vs. SwissProt
Match: FLUG_EMENI (Protein fluG OS=Emericella nidulans GN=fluG PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.525e-9
Identity = 38/106 (35.85%), Postives = 52/106 (49.06%), Query Frame = 2

Query:  449 QPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIA--REGREEWIQFDSTPYCSSSAFDAAS--PILREITSALHSMGIPVEQLHAEAGKGQFELVL 754
            + G++ E CPR  L   +  LKDEF +    GFE E   LK       G E+W    +    S    +     P+L EI   L S+GI ++Q HAE+  GQFE +L
Sbjct:  531 EQGESLEECPRTNLLNINNKLKDEFGIQATCGFEIEVVFLKPTTDPSTGEEDWAPSVTNHSWSQMTRETRRMLPLLEEIAETLASIGIHLQQFHAESAPGQFEFIL 636

[Close]

BLAST of GT627259 vs. SwissProt
Match: GLNA_METVO (Glutamine synthetase OS=Methanococcus voltae GN=glnA PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 3.045e-8
Identity = 35/118 (29.66%), Postives = 53/118 (44.92%), Query Frame = 2

Query:  428 VLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            ++ D+  + G+ +E  PR  L+R     K+EF      G E EFF+LK+       +W+  D   Y      D  + + R I  AL ++G  VE  H E   GQ E+   +    K A
Sbjct:   98 IICDVYRKNGKPFEGDPRGCLKRVLAEFKEEFKGEYFVGPEPEFFILKN----ENGKWVPGDDAGYFELEPLDEGNDLRRNIVFALENLGFHVEASHHEVAPGQHEVDFKYDNAVKTA 211

HSP 2 Score: 26.5646 bits (57), Expect = 3.045e-8
Identity = 23/79 (29.11%), Postives = 37/79 (46.84%), Query Frame = 1

Query:   79 DVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEAR--LTPDLSTIRTIPW 309
            +V F+R  +VD  G+ +  A P K    + T +G       +    F DG +   G   + ++   L PDLST+  +PW
Sbjct:   15 NVKFLRFQFVDIHGEPKNIAYPVK----LGTADGEEELMGVLENGLFFDGSSI-EGFVEIEDSDMVLKPDLSTLSVLPW 88

[Close]

BLAST of GT627259 vs. SwissProt
Match: GLNA_HALVD (Glutamine synthetase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / IFO 14742 / NCIMB 2012 / DS2) GN=glnA PE=3 SV=2)

HSP 1 Score: 49.6766 bits (117), Expect = 2.420e-7
Identity = 34/113 (30.09%), Postives = 52/113 (46.02%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFEL 748
            S    ++    N   G+ +   PR  L+RA +   +E    +N   E EFFL +    +GR   +  D+  Y   +  D AS + R+I   L SMG  +E  H E  +GQ E+
Sbjct:   98 SAAGRLICDVFNTSTGEPFSGDPRGVLKRAIE-RAEELGYDVNVAPEPEFFLFEE-DEDGRATTVTNDAGGYFDLAPKDLASDVRRDIIYGLESMGFDIEASHHEVAEGQHEI 208

HSP 2 Score: 26.1794 bits (56), Expect = 2.420e-7
Identity = 21/91 (23.08%), Postives = 42/91 (46.15%), Query Frame = 1

Query:   40 AEEEVELVNDLET-DVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            ++E   +++++E  +V F+R+ + D  G  +  +IP  +     T+   G+ F   ++  F+             + RL PD ST   +PW
Sbjct:   13 SDEAQAVIDEIEEKNVDFLRLQFTDILGTVKNVSIPASQAEKAFTE---GIYFDGSSIDGFVRIQE--------SDMRLEPDPSTFAVLPW 92

[Close]

BLAST of GT627259 vs. TAIR peptide
Match: AT3G53180.1 (| Symbols: | glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases | chr3:19707068-19711188 FORWARD LENGTH=852)

HSP 1 Score: 177.563 bits (449), Expect = 8.947e-70
Identity = 80/124 (64.52%), Postives = 103/124 (83.06%), Query Frame = 2

Query:  410 SKQDEIVLGDLNVQPGQAWEYCPREALRRASKILKDEFDLVMNAGFENEFFLLKSIAREGREEWIQFDSTPYCSSSAFDAASPILREITSALHSMGIPVEQLHAEAGKGQFELVLGHTICAKAA 781
            +KQ+ +VL D+ ++PG+AW YCPRE LRR +K+LKDEFDLVMNAGFENEF+LLK++ REG+EE++ FD  PYC++S+FDAASPI  +I  AL S+ I VEQ HAE+GKGQFE+ LGHTI + AA
Sbjct:  495 TKQESMVLADMQLKPGEAWGYCPRETLRRVAKVLKDEFDLVMNAGFENEFYLLKNVVREGKEEYMPFDFGPYCATSSFDAASPIFHDIVPALESLNIEVEQFHAESGKGQFEVSLGHTIASHAA 618

HSP 2 Score: 104.76 bits (260), Expect = 8.947e-70
Identity = 52/79 (65.82%), Postives = 56/79 (70.89%), Query Frame = 1

Query:   73 ETDVSFVRILWVDNSGQHRCRAIPRKRFYDVVTKNGVGLAFVSMAMTSFLDGPAHGSGLGSVGEARLTPDLSTIRTIPW 309
            E   SFVRI+WVD SGQ RCRA+  +RF   V KNGVGL F SM MTSF DGPA  S L  VGE RL PDLST +TIPW
Sbjct:  416 EDSSSFVRIIWVDTSGQQRCRAVQAQRFNRSVKKNGVGLTFASMGMTSFTDGPAEESKLTGVGEIRLVPDLSTKQTIPW 494

The following BLAST results are available for this feature:

BLAST of GT627259 vs. TrEMBL
BLAST of GT627259 vs. SwissProt
BLAST of GT627259 vs. TAIR peptide

BLAST of GT627259 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SC91_MEDTR	1.475e-105	90.32	Glutamine synthetase I OS=Medicago truncatula PE=2...	[more]
D7TIU8_VITVI	1.278e-74	70.16	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9S0C8_RICCO	5.249e-70	65.85	Protein fluG, putative OS=Ricinus communis GN=RCOM...	[more]
B9MTI4_POPTR	6.244e-68	65.85	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7LUF4_ARALY	1.062e-67	64.52	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9SCP3_ARATH	1.385e-67	64.52	Nodulin / glutamate-ammonia ligase-like protein OS...	[more]
C5WZH7_SORBI	9.614e-63	55.65	Putative uncharacterized protein Sb01g020230 OS=So...	[more]
B8BH89_ORYSI	2.779e-62	53.23	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q5MD07_SACOF	4.737e-62	54.84	Glutamine synthetase (Fragment) OS=Saccharum offic...	[more]
Q0IX96_ORYSJ	6.160e-62	52.42	Os10g0456500 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of GT627259 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
GLNA1_DICDI	6.657e-14	34.91	Type-1 glutamine synthetase 1 OS=Dictyostelium dis...	[more]
FLUG_EMENI	2.525e-9	35.85	Protein fluG OS=Emericella nidulans GN=fluG PE=1 S...	[more]
GLNA_METVO	3.045e-8	29.66	Glutamine synthetase OS=Methanococcus voltae GN=gl...	[more]
GLNA_HALVD	2.420e-7	30.09	Glutamine synthetase OS=Haloferax volcanii (strain...	[more]

BLAST of GT627259 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G53180.1	8.947e-70	64.52	\| Symbols: \| glutamate-ammonia ligases;catalytics...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.20.70	no description	coord: 25..106 score: 3.4
None	No IPR available	PANTHER	PTHR20852	GLUTAMINE SYNTHETASE	coord: 33..108 score: 2.2
None	No IPR available	PANTHER	PTHR20852:SF8	NODULIN / GLUTAMATE-AMMONIA LIGASE-LIKE PROTEIN	coord: 33..108 score: 2.2
None	No IPR available	PANTHER	PTHR20852	GLUTAMINE SYNTHETASE	coord: 24..146 score: 2.2
None	No IPR available	PANTHER	PTHR20852:SF8	NODULIN / GLUTAMATE-AMMONIA LIGASE-LIKE PROTEIN	coord: 24..146 score: 2.2
None	No IPR available	SEG	seg	seg	coord: 2..19 score
None	No IPR available	SUPERFAMILY	55931	Glutamine synthetase/guanido kinase	coord: 42..146 score: 5.6
None	No IPR available	SEG	seg	seg	coord: 25..38 score: NAcoord: 41..52 score
IPR008146	Glutamine synthetase, catalytic domain	PFAM	PF00120	Gln-synt_C	coord: 42..143 score: 7.2
IPR014746	Glutamine synthetase/guanido kinase, catalytic domain	GENE3D	3.30.590.10	no description	coord: 45..146 score: 7.1

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627259 ID=GT627259; Name=GT627259; organism=Lens culinaris; type=EST; length=781bp
GAGATTAGTGAGAGAATGGATTTGATTGAGTTGAGAAAAGCTGAGGAAGA
AGTTGAACTTGTGAATGATTTAGAGACTGATGTTTCATTTGTTCGTATAT
TATGGGTTGATAATTCAGGCCAACATAGATGTCGAGCAATTCCTAGAAAA
CGTTTCTATGATGTTGTTACAAAGAATGGTGTTGGTTTAGCATTTGTGAG
TATGGCAATGACATCTTTTTTAGATGGACCTGCACATGGTTCTGGTTTGG
GTTCAGTTGGTGAAGCAAGATTAACACCTGATTTGTCTACTATAAGGACA
ATTCCTTGGTACACAACTTCATCATAGTAAAAAATATATATAAACACTTT
CTTTACATTTTCATATATTTTTTCATTCCTTTGGTCTTTTTCTGTTTCAT
TATTATAGGAGCAAGCAAGATGAAATAGTTTTAGGTGATTTGAATGTTCA
ACCTGGTCAAGCATGGGAATATTGTCCAAGAGAAGCCTTAAGAAGAGCTT
CCAAAATTTTGAAAGATGAATTTGATTTGGTAATGAATGCAGGGTTTGAG
AATGAGTTTTTTCTCTTGAAGAGCATAGCAAGGGAAGGAAGAGAAGAATG
GATACAATTTGACTCAACTCCTTACTGTTCCTCTTCTGCATTCGACGCTG
CTTCCCCAATTCTTCGTGAAATTACATCTGCTTTACATTCCATGGGAATT
CCAGTAGAACAGTTACATGCAGAAGCTGGAAAAGGTCAATTCGAATTGGT
TTTAGGACACACCATTTGTGCTAAAGCTGCA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004356	glutamine synthetase activity
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006807	nitrogen compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR008146	Gln_synth_cat_dom
IPR014746	Gln_synth/guanido_kin_cat_dom

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