GT627398

EST Overview


Name	GT627398
Unique Name	GT627398
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	769

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT627398 vs. TrEMBL
Match: C6TEZ7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 1.015e-37
Identity = 74/97 (76.29%), Postives = 83/97 (85.57%), Query Frame = 1

Query:  475 MESTCFGSNVEVSLKQKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYG 765
            MES  F SN EVSLKQK KS + E TIS+ E  CFDCNIC+ESA+DPVVTLCGHLYCWPCIYKWL+VQ+SSVEP +Q TCPVCK+ ISHTS+VPLYG
Sbjct:    1 MESKYFDSNGEVSLKQKSKSISAEPTISTSEYDCFDCNICMESAHDPVVTLCGHLYCWPCIYKWLDVQSSSVEPYQQQTCPVCKSEISHTSVVPLYG 97

[Close]

BLAST of GT627398 vs. TrEMBL
Match: B9SNN5_RICCO (Rnf5, putative OS=Ricinus communis GN=RCOM_1010170 PE=4 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 4.274e-28
Identity = 57/96 (59.38%), Postives = 73/96 (76.04%), Query Frame = 1

Query:  490 FGSNVEVSLKQKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPD---KQPTCPVCKAVISHTSLVPLYGR 768
            F +  ++++KQK KS +    +S  ++ CF CNICL+SANDPVVTLCGHLYCWPCIYKWL V+ +S + D   +QP+CPVCKA IS  S+VPLYGR
Sbjct:   12 FDAEEDLTVKQKWKSVSAATGLSEDDDDCFSCNICLDSANDPVVTLCGHLYCWPCIYKWLQVKRTSSDVDEQQQQPSCPVCKANISSNSMVPLYGR 107

[Close]

BLAST of GT627398 vs. TrEMBL
Match: A9PCH2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_743801 PE=2 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 4.274e-28
Identity = 55/88 (62.50%), Postives = 68/88 (77.27%), Query Frame = 1

Query:  505 EVSLKQKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            E  + Q  KS +   T S   +GCFDCNIC + A++PVVTLCGHLYCWPCIYKWL+VQ++S+  D+ P CPVCKA ISHT++VPLYGR
Sbjct:    4 EKYVTQGWKSVSSAATDSENPSGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLASDEHPQCPVCKADISHTTMVPLYGR 91

[Close]

BLAST of GT627398 vs. TrEMBL
Match: C6TBF7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.244e-27
Identity = 52/85 (61.18%), Postives = 67/85 (78.82%), Query Frame = 1

Query:  514 LKQKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            + +  K+     T +   NGCFDCNICL+ A++PVVTLCGHLYCWPCIYKWL+VQ+ S+ PD+ P CPVCKA IS++++VPLYGR
Sbjct:    7 ISRDLKTIPNAATETENSNGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSDSLAPDEHPQCPVCKADISNSTMVPLYGR 91

[Close]

BLAST of GT627398 vs. TrEMBL
Match: Q8H222_9ROSI (Putative RING protein OS=Populus tremula x Populus alba GN=RING PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.624e-27
Identity = 53/83 (63.86%), Postives = 66/83 (79.52%), Query Frame = 1

Query:  520 QKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            Q  KS +   + S   +GCFDCNIC + A++PVVTLCGHLYCWPCIYKWL+VQ++S+  D+ P CPVCKA ISHT++VPLYGR
Sbjct:    9 QGWKSVSSAASDSENPSGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLASDEHPQCPVCKADISHTTMVPLYGR 91

[Close]

BLAST of GT627398 vs. TrEMBL
Match: B9RZ87_RICCO (Rnf5, putative OS=Ricinus communis GN=RCOM_0936330 PE=4 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.624e-27
Identity = 53/80 (66.25%), Postives = 64/80 (80.00%), Query Frame = 1

Query:  529 KSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            KS     T S   +GCFDCNIC + A++PVVTLCGHLYCWPCIYKWL+VQ++S+  D+ P CPVCKA ISHT++VPLYGR
Sbjct:   12 KSIASAATGSESFSGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASIASDEHPQCPVCKADISHTTMVPLYGR 91

[Close]

BLAST of GT627398 vs. TrEMBL
Match: B9H2V8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_713979 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 3.618e-27
Identity = 53/83 (63.86%), Postives = 64/83 (77.11%), Query Frame = 1

Query:  520 QKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            Q  KS +     S   +GCFDCNIC + AN+PVVTLCGHLYCWPCIYKWL+VQ++S+  D+ P CPVCKA ISH ++VPLYGR
Sbjct:    9 QGWKSVSSAAADSESFSGCFDCNICFDFANEPVVTLCGHLYCWPCIYKWLHVQSASLASDEHPQCPVCKADISHATMVPLYGR 91

[Close]

BLAST of GT627398 vs. TrEMBL
Match: D7T5W2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_916.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00002819001 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 8.913e-26
Identity = 50/80 (62.50%), Postives = 62/80 (77.50%), Query Frame = 1

Query:  529 KSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            +S +   T +   N  F+CNIC +SA DPVVTLCGHLYCWPC+YKW +VQ++S+  D+ P CPVCKA ISHT+LVPLYGR
Sbjct:   12 RSVSAAATEAENLNDSFECNICFDSARDPVVTLCGHLYCWPCVYKWFHVQSASLASDEHPQCPVCKAEISHTTLVPLYGR 91

[Close]

BLAST of GT627398 vs. TrEMBL
Match: A5B310_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013326 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 8.913e-26
Identity = 50/80 (62.50%), Postives = 62/80 (77.50%), Query Frame = 1

Query:  529 KSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            +S +   T +   N  F+CNIC +SA DPVVTLCGHLYCWPC+YKW +VQ++S+  D+ P CPVCKA ISHT+LVPLYGR
Sbjct:   12 RSVSAAATEAENLNDSFECNICFDSARDPVVTLCGHLYCWPCVYKWFHVQSASLASDEHPQCPVCKAEISHTTLVPLYGR 91

[Close]

BLAST of GT627398 vs. TrEMBL
Match: B9N1I4_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_263506 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.164e-25
Identity = 55/85 (64.71%), Postives = 66/85 (77.65%), Query Frame = 1

Query:  517 KQKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVE-PDKQPTCPVCKAVISHTSLVPLYGR 768
            KQK K       +S  ++G F+CNICL+SA+DPVVTLCGHLYCWPCIYKWL+V+ SS +   +QP+CPVCKA IS  SLVPLYGR
Sbjct:    1 KQKHKLIPVPTAVSDNDSGYFECNICLDSAHDPVVTLCGHLYCWPCIYKWLHVKTSSPDASQQQPSCPVCKADISPNSLVPLYGR 85

[Close]

BLAST of GT627398 vs. SwissProt
Match: RMA3_ARATH (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana GN=RMA3 PE=1 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.172e-27
Identity = 57/85 (67.06%), Postives = 67/85 (78.82%), Query Frame = 1

Query:  517 KQKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDK-QPTCPVCKAVISHTSLVPLYGR 768
            KQK   TT      + E+GCFDCNICL++A+DPVVTLCGHL+CWPCIYKWL+VQ SSV  D+ Q  CPVCK+ I+ TSLVPLYGR
Sbjct:   22 KQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGR 106

[Close]

BLAST of GT627398 vs. SwissProt
Match: RMA1_CAPAN (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.937e-22
Identity = 47/84 (55.95%), Postives = 60/84 (71.43%), Query Frame = 1

Query:  523 KCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVE--PDKQPTCPVCKAVISHTSLVPLYGR 768
            K KS  +E  +    +G FDCNICL+  ++PV+TLCGHLYCWPCIYKW+  Q+ S E    +QP CPVCKA +S  +L+PLYGR
Sbjct:   23 KWKSMNDE--VEENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQPQCPVCKAEVSEKTLIPLYGR 104

[Close]

BLAST of GT627398 vs. SwissProt
Match: RMA1_ARATH (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana GN=RMA1 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.639e-21
Identity = 43/66 (65.15%), Postives = 55/66 (83.33%), Query Frame = 1

Query:  577 FDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQ--NSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            FDCNICL+S  +PVVTLCGHL+CWPCI+KWL+VQ  ++S E  +   CPVCK+ +SH++LVPLYGR
Sbjct:   46 FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTLVPLYGR 111

[Close]

BLAST of GT627398 vs. SwissProt
Match: RMA2_ARATH (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana GN=RMA2 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.175e-19
Identity = 43/83 (51.81%), Postives = 53/83 (63.86%), Query Frame = 1

Query:  541 EERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQ-------PTCPVCKAVISHTSLVPLYGR 768
            E+ T      G FDCNICL+   DPVVTLCGHL+CWPCI+KW    N+S +   Q       P CPVCK+ +S  +LVP+YGR
Sbjct:    7 EDDTTLVDSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKSDVSEATLVPIYGR 89

[Close]

BLAST of GT627398 vs. SwissProt
Match: RN185_RAT (RING finger protein 185 OS=Rattus norvegicus GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.447e-18
Identity = 37/71 (52.11%), Postives = 53/71 (74.65%), Query Frame = 1

Query:  556 SSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            S G++  F+CNICL++A D V++LCGHL+CWPC+++WL  +     P++Q  CPVCKA IS   ++PLYGR
Sbjct:   30 SGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETR-----PNRQ-VCPVCKAGISRDKVIPLYGR 94

[Close]

BLAST of GT627398 vs. SwissProt
Match: RN185_PONAB (RING finger protein 185 OS=Pongo abelii GN=RNF185 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.447e-18
Identity = 37/71 (52.11%), Postives = 53/71 (74.65%), Query Frame = 1

Query:  556 SSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            S G++  F+CNICL++A D V++LCGHL+CWPC+++WL  +     P++Q  CPVCKA IS   ++PLYGR
Sbjct:   30 SGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETR-----PNRQ-VCPVCKAGISRDKVIPLYGR 94

[Close]

BLAST of GT627398 vs. SwissProt
Match: RN185_MOUSE (RING finger protein 185 OS=Mus musculus GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.447e-18
Identity = 37/71 (52.11%), Postives = 53/71 (74.65%), Query Frame = 1

Query:  556 SSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            S G++  F+CNICL++A D V++LCGHL+CWPC+++WL  +     P++Q  CPVCKA IS   ++PLYGR
Sbjct:   30 SGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETR-----PNRQ-VCPVCKAGISRDKVIPLYGR 94

[Close]

BLAST of GT627398 vs. SwissProt
Match: RN185_HUMAN (RING finger protein 185 OS=Homo sapiens GN=RNF185 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.447e-18
Identity = 37/71 (52.11%), Postives = 53/71 (74.65%), Query Frame = 1

Query:  556 SSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            S G++  F+CNICL++A D V++LCGHL+CWPC+++WL  +     P++Q  CPVCKA IS   ++PLYGR
Sbjct:   30 SGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETR-----PNRQ-VCPVCKAGISRDKVIPLYGR 94

[Close]

BLAST of GT627398 vs. SwissProt
Match: RNF5_RAT (E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegicus GN=Rnf5 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 8.420e-18
Identity = 39/69 (56.52%), Postives = 51/69 (73.91%), Query Frame = 1

Query:  562 GENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            G +  F+CNICLE+A + VV++CGHLYCWPC+++WL  +     PD+Q  CPVCKA IS   +VPLYGR
Sbjct:   20 GASATFECNICLETAREAVVSVCGHLYCWPCLHQWLETR-----PDRQ-ECPVCKAGISREKVVPLYGR 82

[Close]

BLAST of GT627398 vs. SwissProt
Match: RNF5_MOUSE (E3 ubiquitin-protein ligase RNF5 OS=Mus musculus GN=Rnf5 PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 8.420e-18
Identity = 39/69 (56.52%), Postives = 51/69 (73.91%), Query Frame = 1

Query:  562 GENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            G +  F+CNICLE+A + VV++CGHLYCWPC+++WL  +     PD+Q  CPVCKA IS   +VPLYGR
Sbjct:   20 GASATFECNICLETAREAVVSVCGHLYCWPCLHQWLETR-----PDRQ-ECPVCKAGISREKVVPLYGR 82

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT4G27470.1 (| Symbols: ATRMA3, RMA3 | RING membrane-anchor 3 | chr4:13735576-13736307 FORWARD LENGTH=243)

HSP 1 Score: 123.635 bits (309), Expect = 1.171e-28
Identity = 57/85 (67.06%), Postives = 67/85 (78.82%), Query Frame = 1

Query:  517 KQKCKSTTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDK-QPTCPVCKAVISHTSLVPLYGR 768
            KQK   TT      + E+GCFDCNICL++A+DPVVTLCGHL+CWPCIYKWL+VQ SSV  D+ Q  CPVCK+ I+ TSLVPLYGR
Sbjct:   22 KQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGR 106

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT4G03510.2 (| Symbols: RMA1, ATRMA1 | RING membrane-anchor 1 | chr4:1557905-1558654 REVERSE LENGTH=249)

HSP 1 Score: 103.219 bits (256), Expect = 1.638e-22
Identity = 43/66 (65.15%), Postives = 55/66 (83.33%), Query Frame = 1

Query:  577 FDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQ--NSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            FDCNICL+S  +PVVTLCGHL+CWPCI+KWL+VQ  ++S E  +   CPVCK+ +SH++LVPLYGR
Sbjct:   46 FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTLVPLYGR 111

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT4G03510.1 (| Symbols: RMA1, ATRMA1 | RING membrane-anchor 1 | chr4:1557905-1558654 REVERSE LENGTH=249)

HSP 1 Score: 103.219 bits (256), Expect = 1.638e-22
Identity = 43/66 (65.15%), Postives = 55/66 (83.33%), Query Frame = 1

Query:  577 FDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQ--NSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            FDCNICL+S  +PVVTLCGHL+CWPCI+KWL+VQ  ++S E  +   CPVCK+ +SH++LVPLYGR
Sbjct:   46 FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTLVPLYGR 111

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT1G19310.1 (| Symbols: | RING/U-box superfamily protein | chr1:6676424-6677104 REVERSE LENGTH=226)

HSP 1 Score: 97.4413 bits (241), Expect = 8.987e-21
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 1

Query:  556 SSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            ++ ++  F+CNICL+ A DP+VTLCGHL+CWPC+YKWL++ + S +      CPVCKAVI    LVPLYGR
Sbjct:   14 NNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKD------CPVCKAVIEEDRLVPLYGR 78

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT4G28270.1 (| Symbols: ATRMA2, RMA2 | RING membrane-anchor 2 | chr4:14007614-14008195 REVERSE LENGTH=193)

HSP 1 Score: 97.0561 bits (240), Expect = 1.174e-20
Identity = 43/83 (51.81%), Postives = 53/83 (63.86%), Query Frame = 1

Query:  541 EERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQ-------PTCPVCKAVISHTSLVPLYGR 768
            E+ T      G FDCNICL+   DPVVTLCGHL+CWPCI+KW    N+S +   Q       P CPVCK+ +S  +LVP+YGR
Sbjct:    7 EDDTTLVDSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKSDVSEATLVPIYGR 89

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT2G23780.1 (| Symbols: | RING/U-box superfamily protein | chr2:10123551-10124234 REVERSE LENGTH=227)

HSP 1 Score: 97.0561 bits (240), Expect = 1.174e-20
Identity = 41/79 (51.90%), Postives = 54/79 (68.35%), Query Frame = 1

Query:  532 STTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            S ++    ++ + G F+CNIC E A DP+VTLCGHL+CWPC+Y+WL+  + S E      CPVCKAV+    LVPLYGR
Sbjct:   11 SYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQE------CPVCKAVVQDDKLVPLYGR 83

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT1G74990.1 (| Symbols: | RING/U-box superfamily protein | chr1:28159837-28160250 REVERSE LENGTH=137)

HSP 1 Score: 90.8929 bits (224), Expect = 8.412e-19
Identity = 41/77 (53.25%), Postives = 51/77 (66.23%), Query Frame = 1

Query:  535 TTEERTISSGENGCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYG 765
            T EE   S+     F CNICLE A +P+VTLCGHL+CWPC+YKWL+  +      K   CPVCKA++   +LVPLYG
Sbjct:    7 TNEEDDASNN----FGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHS------KSNHCPVCKALVKEDTLVPLYG 73

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT3G58030.4 (| Symbols: | RING/U-box superfamily protein | chr3:21485527-21486837 FORWARD LENGTH=436)

HSP 1 Score: 87.0409 bits (214), Expect = 1.215e-17
Identity = 35/72 (48.61%), Postives = 50/72 (69.44%), Query Frame = 1

Query:  556 SSGENG-CFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            SSG +G  FDCNICL+ + +PV+T CGHLYCWPC+Y+WL + ++         CPVCK  ++  ++ P+YGR
Sbjct:  129 SSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQISDAK-------ECPVCKGEVTSKTVTPIYGR 193

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT3G58030.3 (| Symbols: | RING/U-box superfamily protein | chr3:21485527-21486837 FORWARD LENGTH=436)

HSP 1 Score: 87.0409 bits (214), Expect = 1.215e-17
Identity = 35/72 (48.61%), Postives = 50/72 (69.44%), Query Frame = 1

Query:  556 SSGENG-CFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            SSG +G  FDCNICL+ + +PV+T CGHLYCWPC+Y+WL + ++         CPVCK  ++  ++ P+YGR
Sbjct:  129 SSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQISDAK-------ECPVCKGEVTSKTVTPIYGR 193

[Close]

BLAST of GT627398 vs. TAIR peptide
Match: AT3G58030.2 (| Symbols: | RING/U-box superfamily protein | chr3:21485527-21486837 FORWARD LENGTH=436)

HSP 1 Score: 87.0409 bits (214), Expect = 1.215e-17
Identity = 35/72 (48.61%), Postives = 50/72 (69.44%), Query Frame = 1

Query:  556 SSGENG-CFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQNSSVEPDKQPTCPVCKAVISHTSLVPLYGR 768
            SSG +G  FDCNICL+ + +PV+T CGHLYCWPC+Y+WL + ++         CPVCK  ++  ++ P+YGR
Sbjct:  129 SSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQISDAK-------ECPVCKGEVTSKTVTPIYGR 193

The following BLAST results are available for this feature:

BLAST of GT627398 vs. TrEMBL
BLAST of GT627398 vs. SwissProt
BLAST of GT627398 vs. TAIR peptide

BLAST of GT627398 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TEZ7_SOYBN	1.015e-37	76.29	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9SNN5_RICCO	4.274e-28	59.38	Rnf5, putative OS=Ricinus communis GN=RCOM_1010170...	[more]
A9PCH2_POPTR	4.274e-28	62.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C6TBF7_SOYBN	1.244e-27	61.18	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q8H222_9ROSI	1.624e-27	63.86	Putative RING protein OS=Populus tremula x Populus...	[more]
B9RZ87_RICCO	1.624e-27	66.25	Rnf5, putative OS=Ricinus communis GN=RCOM_0936330...	[more]
B9H2V8_POPTR	3.618e-27	63.86	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7T5W2_VITVI	8.913e-26	62.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5B310_VITVI	8.913e-26	62.50	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9N1I4_POPTR	1.164e-25	64.71	Predicted protein (Fragment) OS=Populus trichocarp...	[more]

BLAST of GT627398 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RMA3_ARATH	1.172e-27	67.06	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis th...	[more]
RMA1_CAPAN	1.937e-22	55.95	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum ann...	[more]
RMA1_ARATH	1.639e-21	65.15	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis th...	[more]
RMA2_ARATH	1.175e-19	51.81	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis th...	[more]
RN185_RAT	6.447e-18	52.11	RING finger protein 185 OS=Rattus norvegicus GN=Rn...	[more]
RN185_PONAB	6.447e-18	52.11	RING finger protein 185 OS=Pongo abelii GN=RNF185 ...	[more]
RN185_MOUSE	6.447e-18	52.11	RING finger protein 185 OS=Mus musculus GN=Rnf185 ...	[more]
RN185_HUMAN	6.447e-18	52.11	RING finger protein 185 OS=Homo sapiens GN=RNF185 ...	[more]
RNF5_RAT	8.420e-18	56.52	E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegi...	[more]
RNF5_MOUSE	8.420e-18	56.52	E3 ubiquitin-protein ligase RNF5 OS=Mus musculus G...	[more]

BLAST of GT627398 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G27470.1	1.171e-28	67.06	\| Symbols: ATRMA3, RMA3 \| RING membrane-anchor 3 \|...	[more]
AT4G03510.2	1.638e-22	65.15	\| Symbols: RMA1, ATRMA1 \| RING membrane-anchor 1 \|...	[more]
AT4G03510.1	1.638e-22	65.15	\| Symbols: RMA1, ATRMA1 \| RING membrane-anchor 1 \|...	[more]
AT1G19310.1	8.987e-21	56.34	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT4G28270.1	1.174e-20	51.81	\| Symbols: ATRMA2, RMA2 \| RING membrane-anchor 2 \|...	[more]
AT2G23780.1	1.174e-20	51.90	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT1G74990.1	8.412e-19	53.25	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT3G58030.4	1.215e-17	48.61	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT3G58030.3	1.215e-17	48.61	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT3G58030.2	1.215e-17	48.61	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	no description	coord: 62..108 score: 2.7
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 62..109 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	no description	coord: 60..119 score: 1.8
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 77..86 score
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 62..108 score: 3.7
None	No IPR available	PANTHER	PTHR12313	RNF5	coord: 56..123 score: 1.7
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 36..115 score: 6.9

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627398 ID=GT627398; Name=GT627398; organism=Lens culinaris; type=EST; length=769bp
GCTCTTCTATATGGATGTTGTTGCCTGAATTTTTTAATGTCCCTTATTTC
TCTTTGGAATCATCAACTTACTTTCTGATAAAGGATGATTGTTTGGTATA
TGGTTATTGGCCTGCTGCTTCCATACCATTATTCTATTTGAAAATGACCA
ATGAACATATAAATTTAATCTATCGTGTAGATTCCATGTTACAAATGATT
CTGGGGACCTTGTATGGATAAAAGTTTTCATATCTTAAGAAATCAACATG
GTAAATTATCTGTTCGTATGATGTGATTTCGGTTTTCAATATCATTAGTT
TTGTTTTTCTTCTTCCTATATTTGCCATAGAATCATAAGAAATGTTGTAT
CTTGTGTCATCTGAGTACCTGATCTAATTGATTCTACTTCTGCATCTGAA
TTGTCTCTAATGATTGCAGTTTTCTGAAATTTGTAAAATCTATAACAGGG
TATATTTCTGTAGTAGAAAATTCAATGGAATCTACATGCTTTGGTTCTAA
TGTGGAAGTCTCACTAAAGCAAAAATGTAAATCTACTACAGAAGAGAGAA
CAATCTCTAGTGGTGAAAATGGTTGCTTTGATTGTAATATTTGCTTGGAA
TCAGCAAATGATCCTGTGGTTACACTTTGTGGTCATCTCTATTGCTGGCC
ATGCATATATAAATGGCTTAATGTCCAAAACTCTTCAGTTGAACCAGATA
AGCAGCCTACATGCCCTGTTTGTAAAGCTGTCATCTCCCATACTTCATTA
GTCCCTCTTTATGGCCGTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD
IPR017907	Znf_RING_CS
IPR018957	Znf_C3HC4_RING-type

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