GT620501

EST Overview


Name	GT620501
Unique Name	GT620501
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	797

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT620501 vs. TrEMBL
Match: B9H5N0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_760229 PE=4 SV=1)

HSP 1 Score: 414.075 bits (1063), Expect = 7.143e-114
Identity = 184/242 (76.03%), Postives = 219/242 (90.50%), Query Frame = 3

Query:   75 HHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHDSAPA-HESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            HH+PVPFDPLVA++ER REDK+WEKQ  EHSH E +HDSA A HESQPEWEDF++AED++NDE+KFNVT+RLL+LFPKIDV+P D ++  HELT+WNL+Q+ +EV+HR++REM++HDKNHDGFVSF+EY+PPSWV+++DK+SFGYDMGWWKEEHFNASDADGDG LN+TEFNDF HPADS N KL QWLCKEEVRERDSD+DGKVNF+EFFHGLFDLVRNYD EE H  SH SDN M+APA+
Sbjct:   51 HHEPVPFDPLVADIERKREDKEWEKQYFEHSHPEFVHDSAAAGHESQPEWEDFIDAEDYVNDEEKFNVTNRLLVLFPKIDVEPDDGYVSEHELTEWNLKQSEKEVMHRTKREMDVHDKNHDGFVSFAEYEPPSWVRNSDKNSFGYDMGWWKEEHFNASDADGDGFLNITEFNDFQHPADSKNPKLLQWLCKEEVRERDSDKDGKVNFQEFFHGLFDLVRNYD-EEGHNSSHLSDNLMEAPAK 291

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BLAST of GT620501 vs. TrEMBL
Match: B9RGH6_RICCO (Reticulocalbin-2, putative OS=Ricinus communis GN=RCOM_1454090 PE=4 SV=1)

HSP 1 Score: 408.683 bits (1049), Expect = 3.001e-112
Identity = 182/243 (74.90%), Postives = 217/243 (89.30%), Query Frame = 3

Query:   75 HHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHD--SAPAHESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            HH+PVPFDPLVA++ER REDKQWEK  IE SH ++L D  +AP HE QPEWEDFM+AED++NDE+KFNVTSRLL+LFPKIDVDPVD  +  HELT+WN+ QA REV+HR+QRE+E+HDKNHDG +SFSEY+PPSWV ++D+++FGYDMGWW+EEHFNASDADGDG+LN+TEFNDFLHPADS N KL QWLC EEVRERDSD+DGKVNFKEFFHGLFDLVRNYD EE+H  SH +D+S++APA+
Sbjct:   50 HHEPVPFDPLVADIERRREDKQWEKHYIESSHPDILKDIDAAPGHEPQPEWEDFMDAEDYLNDEEKFNVTSRLLLLFPKIDVDPVDGHVSEHELTEWNMDQAKREVMHRTQREVEVHDKNHDGLISFSEYEPPSWVHNSDQNTFGYDMGWWREEHFNASDADGDGLLNITEFNDFLHPADSKNPKLLQWLCMEEVRERDSDKDGKVNFKEFFHGLFDLVRNYD-EESHNSSHPTDDSLEAPAK 291

[Close]

BLAST of GT620501 vs. TrEMBL
Match: B9HGD1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_819878 PE=4 SV=1)

HSP 1 Score: 404.445 bits (1038), Expect = 5.660e-111
Identity = 178/251 (70.92%), Postives = 223/251 (88.84%), Query Frame = 3

Query:   48 NFTLSPSHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHDSA-PAHESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            +F   P H HH+PVPFDPLVA++ER REDK+WE+Q +E+SH EL+H SA P HESQPE ++FM+AED++NDE+KFNVT+RLL+LFPKID++PVD ++  HELT+W+L+Q+ +EV+HR++REM++HDKNHDG +SF+EY+PPSWV ++DK+SFGYDMGWWKEEHFNASDADGDG+LN+TEFNDF HPADS N KL QWLCKEEVRERDSD+DGKVNF+EFFHGLFD VRNYD EE H  SH SD+S++APA+
Sbjct:   40 DFAFDPRHHHHEPVPFDPLVADIERKREDKEWERQYLENSHPELVHHSAAPGHESQPEVDNFMDAEDYLNDEEKFNVTNRLLVLFPKIDMEPVDGYLSEHELTEWSLKQSEKEVMHRTKREMDVHDKNHDGLISFAEYEPPSWVHNSDKNSFGYDMGWWKEEHFNASDADGDGLLNITEFNDFQHPADSKNPKLLQWLCKEEVRERDSDKDGKVNFQEFFHGLFDSVRNYD-EEGHNSSHLSDDSVEAPAK 289

[Close]

BLAST of GT620501 vs. TrEMBL
Match: D7TXZ7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_71.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034380001 PE=4 SV=1)

HSP 1 Score: 382.874 bits (982), Expect = 1.763e-104
Identity = 175/250 (70.00%), Postives = 211/250 (84.40%), Query Frame = 3

Query:   48 NFTLSPSHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHDSAPAHESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            ++T + ++ H   V FDP+VA++ER REDK+WEK + ++ H E   DSAPA ESQPEWEDFMNAED++NDED+FN+T RL++LFPKIDVDP D F+   ELTQWNL+Q+ +EVLHR+QREMELHDKN DG VSFSEY+ PSWV+++D  SFGYDMGWWKEEHFNASDADGDG+LN+TEFNDFLHPADS N KL QWLCKEE+RERD+D+DGKVNF EFFHGLFDLVRNY+ EE H  SH S + M+APA+
Sbjct:   44 SYTFTGANDHR--VAFDPIVADIERRREDKEWEKLQFQNRHPEF--DSAPAAESQPEWEDFMNAEDYLNDEDRFNITGRLVLLFPKIDVDPEDGFVSESELTQWNLKQSEKEVLHRTQREMELHDKNKDGLVSFSEYEAPSWVRNSDNTSFGYDMGWWKEEHFNASDADGDGLLNITEFNDFLHPADSKNPKLVQWLCKEEIRERDTDKDGKVNFNEFFHGLFDLVRNYN-EEGHNSSHESSDLMEAPAK 288

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BLAST of GT620501 vs. TrEMBL
Match: D7M1F9_ARALY (Calcium-binding EF hand family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_325456 PE=4 SV=1)

HSP 1 Score: 372.859 bits (956), Expect = 1.825e-101
Identity = 166/261 (63.60%), Postives = 210/261 (80.46%), Query Frame = 3

Query:   48 NFTLSPSHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELL-----------HDSAPAHESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            +F   P+   H PVPFDPLVA++ER REDK+WE+Q I+HSH EL+           H+ AP HESQPEWEDFM+AED++NDE+KFNVT RL++LFPKIDV P D F+   ELT+W +Q + +EV+HR+QR++++HD+N DGF+SFSEY+PPSWV+ +D +SFGYDMGWWKEEHFNASDA+GDG+LNLTEFNDFLHPAD+ N KL  WLCKEEVRERDSD+DGK++F+EFFHGLFD VRNY EE+ H  +H   +  + PA+
Sbjct:   48 SFNFKPTR--HDPVPFDPLVADMERRREDKEWERQYIDHSHPELVSHSQKETTGGGHEHAPGHESQPEWEDFMDAEDYLNDEEKFNVTDRLMLLFPKIDVSPTDGFVTESELTEWTMQSSAKEVVHRTQRDLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTEFNDFLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNY-EEDNHNSTHPYHDLPEGPAK 305

[Close]

BLAST of GT620501 vs. TrEMBL
Match: Q9FNN0_ARATH (Genomic DNA, chromosome 5, P1 clone:MAH20 OS=Arabidopsis thaliana GN=MAH20.14 PE=2 SV=1)

HSP 1 Score: 371.703 bits (953), Expect = 4.065e-101
Identity = 166/261 (63.60%), Postives = 210/261 (80.46%), Query Frame = 3

Query:   48 NFTLSPSHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELL-----------HDSAPAHESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            +F   P+   H PVPFDPLVA++ER REDK+WE+Q IEHSH EL+           H+ AP HESQPEWE+FM+AED++NDE+KFNVT RL++LFPKIDV P D F+   ELT+W +Q + +EV+HR+QR++++HD+N DGF+SFSEY+PPSWV+ +D +SFGYDMGWWKEEHFNASDA+GDG+LNLTEFNDFLHPAD+ N KL  WLCKEEVRERDSD+DGK++F+EFFHGLFD VRNY EE+ H  +H   +  + PA+
Sbjct:   48 SFNFKPTR--HDPVPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFMDAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTEFNDFLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNY-EEDNHNSTHPYHDLPEGPAK 305

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BLAST of GT620501 vs. TrEMBL
Match: C5XYF3_SORBI (Putative uncharacterized protein Sb04g007870 OS=Sorghum bicolor GN=Sb04g007870 PE=4 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 6.764e-72
Identity = 137/250 (54.80%), Postives = 169/250 (67.60%), Query Frame = 3

Query:   66 SHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWV-KSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            +H HH  +PFDP+VA++ER  ED++WE+      H       AP  E+ P  ED  + E     D+IND  +FNVT R+  LFPKIDVDP D  +   EL  WNL  A REVLHR+ RE+ELHD++HDG V+F EY+ PSW  +  D +S    +GWWKEEHFNA+D DGDG LNLTEFNDFLHPAD+ N KL  WLCKEEVRERD D DGK+NF+EFF GLF  +R+YD+E    D+  S    DAPA+
Sbjct:   59 AHVHHHQIPFDPVVADIERRIEDREWERLAAAGLH-------APGMEAAPVPEDLTDGEADADEDYINDAARFNVTHRVEALFPKIDVDPADGAVTGAELAAWNLANARREVLHRTARELELHDRDHDGRVAFGEYERPSWAWRFDDHNSTNDGVGWWKEEHFNAADMDGDGFLNLTEFNDFLHPADTTNPKLINWLCKEEVRERDKDNDGKLNFQEFFSGLFYSIRHYDDEGITDDTGGS----DAPAK 297

[Close]

BLAST of GT620501 vs. TrEMBL
Match: B8AE92_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_06410 PE=4 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 1.507e-71
Identity = 136/254 (53.54%), Postives = 173/254 (68.11%), Query Frame = 3

Query:   66 SHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHDSAPAHESQPEWEDFMNA---------EDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWV-KSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            S S    +PFDP++A LER R+D++WE+      H       AP  E+ P  ED+++          ED+IND  +FN+T R+  LFPKIDVDP D  +   ELT WNL  A REV+HR+ RE++LHD++HDG ++FSEY+ PSW  +  D +S    +GWWKEEHFNASD DGDG LNLTEFNDFLHPAD+ N KL  WLCKEEVRERD D DGK+NF+EF++GLF  +R++DEE +  DS    N  DAPAR
Sbjct:   59 SPSPRGQIPFDPVIAGLERRRDDREWERLAAAGLH-------APGFEAAPVPEDYIDGGGGFGADPDEDYINDAARFNLTRRVEALFPKIDVDPADGAVTPAELTAWNLASARREVMHRTARELDLHDRDHDGRIAFSEYERPSWAWRFDDHNSSNDGVGWWKEEHFNASDMDGDGFLNLTEFNDFLHPADTTNPKLINWLCKEEVRERDKDNDGKLNFQEFYNGLFYSIRHFDEEASTDDS----NVSDAPAR 301

[Close]

BLAST of GT620501 vs. TrEMBL
Match: B4FVI6_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 273.092 bits (697), Expect = 1.968e-71
Identity = 135/250 (54.00%), Postives = 171/250 (68.40%), Query Frame = 3

Query:   66 SHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWV-KSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            +H+HH  +PFDP+VA++ER  ED++WE+      H       AP  E+ P  ED  + E     D+IND  +FNVT R+  LFPKIDVDP D  +   EL  WNL  A REVLHR+ RE+ELHD++HDG V++ EY+ PSW  +  D +S    +GWWKEEHF+A+D DGDG LNLTEFNDFLHPAD+ N KL  WLCKEEVRERD D DGK+NF+EFF+GLF  +R+YD+E    D+  S    DAPA+
Sbjct:   57 AHAHHHQIPFDPVVADIERRLEDREWERLAAAGLH-------APGMEAAPVPEDLTDGEVDADEDYINDAARFNVTLRVEALFPKIDVDPADGAVTGAELAAWNLANARREVLHRTARELELHDRDHDGRVAYGEYERPSWAWRFDDHNSTDDGVGWWKEEHFSAADMDGDGFLNLTEFNDFLHPADTTNPKLIHWLCKEEVRERDKDNDGKLNFQEFFNGLFYSIRHYDDEGLTDDTGGS----DAPAK 295

[Close]

BLAST of GT620501 vs. TrEMBL
Match: Q0E2Q0_ORYSJ (Os02g0221800 protein OS=Oryza sativa subsp. japonica GN=Os02g0221800 PE=4 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.357e-71
Identity = 135/254 (53.15%), Postives = 173/254 (68.11%), Query Frame = 3

Query:   66 SHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELLHDSAPAHESQPEWEDFMNA---------EDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWV-KSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            S S    +PFDP++A LER R+D++WE+      H       AP  E+ P  ED+++          ED+IND  +FN+T R+  LFPKIDVDP D  +   ELT WNL  A REV+HR+ RE++LHD++HDG ++FSEY+ PSW  +  D +S    +GWWKEEHFNASD DGDG LNL EFNDFLHPAD+ N KL  WLCKEEVRERD D DGK+NF+EF++GLF  +R++DEE +  DS    N+ DAPAR
Sbjct:   59 SPSPRGQIPFDPVIAGLERRRDDREWERLAAAGLH-------APGFEAAPVPEDYIDGGGGFGADPDEDYINDAARFNLTRRVEALFPKIDVDPADGAVTPAELTAWNLASARREVMHRTARELDLHDRDHDGRIAFSEYERPSWAWRFDDHNSSNDGVGWWKEEHFNASDMDGDGFLNLIEFNDFLHPADTTNPKLINWLCKEEVRERDKDNDGKLNFQEFYNGLFYSIRHFDEEASTDDS----NASDAPAR 301

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BLAST of GT620501 vs. SwissProt
Match: CALU_RABIT (Calumenin OS=Oryctolagus cuniculus GN=CALU PE=1 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 3.297e-12
Identity = 60/213 (28.17%), Postives = 97/213 (45.54%), Query Frame = 3

Query:  144 EKQRIEHSHL--ELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGYDMGWWKEE-HFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            +K R+ H     + +HD A + +   + + F+ AE     D +  E+      RL  +  KID D  D F+ V EL  W      R +    +R+ + HD N DG VS+ EY   ++    D     D F Y     ++E  F  +D DGD +    EF  FLHP + +  K    + +E + + D + DG ++ +E+   ++    N DE E
Sbjct:   24 KKDRVHHEPQLSDKVHDDAQSFDY--DHDAFLGAEEAKTFDQLTPEES---KERLGKIVSKIDADK-DGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMK--DIVVQETMEDIDKNGDGFIDLEEYIGDMYSHDGNADEPE 228

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALU_PONAB (Calumenin OS=Pongo abelii GN=CALU PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.636e-11
Identity = 59/213 (27.70%), Postives = 97/213 (45.54%), Query Frame = 3

Query:  144 EKQRIEHSHL--ELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGYDMGWWKEE-HFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            +K R+ H     + +H+ A + +   + + F+ AE     D +  E+      RL  +  KID D  D F+ V EL  W      R +    +R+ + HD N DG VS+ EY   ++    D     D F Y     ++E  F  +D DGD +    EF  FLHP + +  K    + +E + + D + DG ++ +E+   ++    N DE E
Sbjct:   24 KKDRVHHEPQLSDKVHNDAQSFDY--DHDAFLGAEEAKTFDQLTPEES---KERLGKIVSKID-DDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMK--DIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPE 228

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALU_HUMAN (Calumenin OS=Homo sapiens GN=CALU PE=1 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 2.137e-11
Identity = 59/213 (27.70%), Postives = 97/213 (45.54%), Query Frame = 3

Query:  144 EKQRIEHSHL--ELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGYDMGWWKEE-HFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            +K R+ H     + +H+ A + +   + + F+ AE     D +  E+      RL  +  KID D  D F+ V EL  W      R +    +R+ + HD N DG VS+ EY   ++    D     D F Y     ++E  F  +D DGD +    EF  FLHP + +  K    + +E + + D + DG ++ +E+   ++    N DE E
Sbjct:   24 KKDRVHHEPQLSDKVHNDAQSFDY--DHDAFLGAEEAKTFDQLTPEES---KERLGKIVSKIDGDK-DGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMK--DIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPE 228

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALU_MOUSE (Calumenin OS=Mus musculus GN=Calu PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.791e-11
Identity = 58/203 (28.57%), Postives = 91/203 (44.83%), Query Frame = 3

Query:  201 HESQPEWEDFMNAEDFINDEDKF---------------NVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRS-QREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGYDMGWWKEE-HFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            HE Q   +   +A++F  D D F                   RL  +  KID D  D F+ V EL  W ++ A +  +H   +R+ + HD N DG VS+ EY   ++    D     D F Y     ++E  F  +D DGD +    EF  FLHP + +  K    + +E + + D + DG ++ +E+   ++    N DE E
Sbjct:   30 HEPQLSDKVHNDAQNFDYDHDAFLGAEEAKSFDQLTPEESKERLGKIVSKID-DDKDGFVTVDELKGW-IKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMK--DIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPE 228

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALUB_SALSA (Calumenin-B OS=Salmo salar GN=calub PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 6.218e-11
Identity = 61/233 (26.18%), Postives = 97/233 (41.63%), Query Frame = 3

Query:  144 EKQRIEHSHLELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGY-DMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLF---------DLVRNYDEEETHTDSHHSDNSMD 785
            +K R+ H       D   A     + E F+  +     D +  E+      RL +L  +ID D  D ++ V E+ +W      R +     R+ + HD N DG VS+ EY   ++    D    +D F Y  M    E  F  SD D D   N  EF  FLHP + ++ K    +  E + + D + DG ++ +E+   ++         + VR   E+ T     + D  MD
Sbjct:   25 KKDRVHHDDPLSSRDHEDAENFDYDHEAFLGQDEAKTFDQLTPEES---KERLGMLVERIDEDK-DGYVSVEEMKKWIKHSQKRWIYDDVDRQWKGHDHNGDGLVSWEEYKNATYGYILDDPDPEDGFSYRQMISRDERRFKMSDLDADLKANKEEFTAFLHPEEYDHMK--DIVVLETMEDIDKNGDGFIDLEEYIGDMYNQEGDPSEPEWVRTEREQFTEFRDTNKDGRMD 251

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALUB_DANRE (Calumenin-B OS=Danio rerio GN=calub PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.385e-10
Identity = 59/224 (26.34%), Postives = 96/224 (42.86%), Query Frame = 3

Query:  183 HDSAPAHESQPEWEDF-MNAEDFINDEDKFNVTS--------RLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSW---VKSAD-KDSFGY-DMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLF---------DLVRNYDEEETHTDSHHSDNSMD 785
            HD+  +++   + E+F  + E F+  E+              RL  +  KID D  D F+   E+ +W      R +     R+ + HD N D FVS+ EY   ++   +  AD +D F Y  M    E  F  +D DGD   N  EF  FLHP + +  K    +  E + + D + DG ++  E+   ++         + V+   E+ T     + D  MD
Sbjct:   30 HDAPLSNKDHDDEENFDYDHEAFLGQEEAKTFDQLTPEESKERLGKIVEKIDEDH-DGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKDATYGYILDEADPEDGFNYRQMMTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMK--DIVVLETMEDIDKNGDGLIDLNEYIGDMYSQNGDSSEPEWVKTEREQFTEFRDKNKDGRMD 250

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALU_MESAU (Calumenin OS=Mesocricetus auratus GN=CALU PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.363e-10
Identity = 57/203 (28.08%), Postives = 90/203 (44.33%), Query Frame = 3

Query:  201 HESQPEWEDFMNAEDFINDEDKF---------------NVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRS-QREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGYDMGWWKEE-HFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            HE Q   +   +A++F  D D F                   RL  +  KID D  D F+ V EL  W ++ A +  +H   +R+ + HD N DG VS+ EY   ++    D     D F Y     ++E  F  +D DGD +    EF  F HP + +  K    + +E + + D + DG ++ +E+   ++    N DE E
Sbjct:   30 HEPQLSDKVHNDAQNFDYDHDAFLGAEEAKSFDQLTPEESKERLGKIVSKID-DDKDGFVTVDELKGW-IKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFPHPDEYDYMK--DIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPE 228

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALU_XENTR (Calumenin OS=Xenopus tropicalis GN=calu PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.086e-10
Identity = 57/213 (26.76%), Postives = 99/213 (46.48%), Query Frame = 3

Query:  144 EKQRIEHSHL--ELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGYDMGWWKEE-HFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            +K R+ H     E +HD A   +   + + F+ AE     D +  E+      RL ++  KID+D  D ++   ELT W  +   + V    +R+ +  D N DG VS+ EY   ++    D     +SF Y     ++E  F  +D DGD +    EF  FLHP + +  K    +  E + + D + DG ++ +E+   +++   + +E E
Sbjct:   24 KKDRVHHDPQLSEKVHDDAQNFDY--DHDAFLGAEEAKTFDQLTPEES---KERLGMIVSKIDLDN-DGYVTEGELTAWIKKAQKKYVYDNVERQWQEFDLNQDGLVSWDEYRNVTYGTYLDDPDPDNSFNYKQMMVRDERRFKMADQDGDLIATKEEFTAFLHPEEFDYMK--DIVVLETMEDIDKNGDGLIDLEEYIGDMYNHDGDANEPE 228

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALU_BOVIN (Calumenin OS=Bos taurus GN=CALU PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 5.264e-10
Identity = 54/213 (25.35%), Postives = 95/213 (44.60%), Query Frame = 3

Query:  144 EKQRIEHSHL--ELLHDSAPAHESQPEWEDFMNAE-----DFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADK----DSFGYDMGWWKEEH-FNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            +K R+ H     + +H+ A + +   + + F+ AE     D +  E+      RL ++  KID D  D F+   EL  W      + +    + + +  D N DG +S+ EY   ++    D     D F Y     ++E  F  +D DGD +    EF  FLHP + +  K    + +E + + D + DG ++ +E+   ++    N DE E
Sbjct:   24 KKDRVHHEPQLSDKVHNDAQSFDY--DHDAFLGAEEAKTFDQLTPEES---KERLGMIVDKIDADK-DGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDLNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMK--DIVVQEPMEDIDKNADGFIDLEEYIGDMYSHDGNADEPE 228

[Close]

BLAST of GT620501 vs. SwissProt
Match: CALU_XENLA (Calumenin OS=Xenopus laevis GN=calu PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 6.874e-10
Identity = 56/212 (26.42%), Postives = 97/212 (45.75%), Query Frame = 3

Query:  144 EKQRIEHSHL--ELLHDSAPA----HESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSAD----KDSFGYDMGWWKEE-HFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEE 746
            +K R+ H     + +HD A      H++    ED    +    +E K     RL ++  KID+D  D ++   ELT W  +   + V    +R+ +  D + DG VS+ EY   ++    D     +SF Y     ++E  F  +D DGD V    EF  FLHP + +  K    +  E + + D + DG ++ +E+   +++   + +E E
Sbjct:   24 KKDRVHHDSQLSDKVHDDAQNFDYDHDAFLGAEDAKTFDQLTPEESK----ERLGMIVGKIDLDN-DGYVTEGELTAWIKKAQKKYVYDNVERQWQEFDLSQDGLVSWDEYRNVTYGTYLDDQDPDNSFNYKQMMIRDERRFKMADKDGDLVATKEEFTAFLHPEEFDYMK--DIVVLETMEDIDKNGDGLIDLEEYIGDMYNHDGDANEPE 228

[Close]

BLAST of GT620501 vs. TAIR peptide
Match: AT5G08580.1 (| Symbols: | Calcium-binding EF hand family protein | chr5:2780976-2782961 REVERSE LENGTH=391)

HSP 1 Score: 371.703 bits (953), Expect = 2.586e-103
Identity = 166/261 (63.60%), Postives = 210/261 (80.46%), Query Frame = 3

Query:   48 NFTLSPSHSHHQPVPFDPLVAELERNREDKQWEKQRIEHSHLELL-----------HDSAPAHESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGYDMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSHHSDNSMDAPAR 797
            +F   P+   H PVPFDPLVA++ER REDK+WE+Q IEHSH EL+           H+ AP HESQPEWE+FM+AED++NDE+KFNVT RL++LFPKIDV P D F+   ELT+W +Q + +EV+HR+QR++++HD+N DGF+SFSEY+PPSWV+ +D +SFGYDMGWWKEEHFNASDA+GDG+LNLTEFNDFLHPAD+ N KL  WLCKEEVRERDSD+DGK++F+EFFHGLFD VRNY EE+ H  +H   +  + PA+
Sbjct:   48 SFNFKPTR--HDPVPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFMDAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTEFNDFLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNY-EEDNHNSTHPYHDLPEGPAK 305

[Close]

BLAST of GT620501 vs. TAIR peptide
Match: AT4G27790.1 (| Symbols: | Calcium-binding EF hand family protein | chr4:13850066-13851488 FORWARD LENGTH=345)

HSP 1 Score: 145.591 bits (366), Expect = 3.014e-35
Identity = 75/225 (33.33%), Postives = 124/225 (55.11%), Query Frame = 3

Query:   93 FDPLVAELERNREDKQWEKQRIEHSHLELLHDSAPAHESQPEWEDFMNAEDFINDEDKFNVTSRLLILFPKIDVDPVDSFIDVHELTQWNLQQAHREVLHRSQREMELHDKNHDGFVSFSEYDPPSWVKSADKDSFGY-DMGWWKEEHFNASDADGDGVLNLTEFNDFLHPADSNNTKLQQWLCKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDEEETHTDSH 764
            FDPLV  +ER   +K+   + +E               ++ E +D    E++   E + N T R+  LFP +D  P D F+ + EL  W +QQ    +++R+ +E+EL DK+ DG ++F EY P    +  +K+  G+ + GWW E+ F  SD D +G L++ EFN+FLHP DS N   Q+W+ KE +   D++ DGK+ +KEF    +++ + + + E   D +
Sbjct:   48 FDPLVTRIERLSHEKEAGTKTVE--------------AAKEEKDDMF--EEYFAQERRLNTTMRIKFLFPLLDASPRDGFVSLKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEAGWWMEQ-FKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKEFVKNAYEMYKEFAKFEKEEDEN 255

The following BLAST results are available for this feature:

BLAST of GT620501 vs. TrEMBL
BLAST of GT620501 vs. SwissProt
BLAST of GT620501 vs. TAIR peptide

BLAST of GT620501 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9H5N0_POPTR	7.143e-114	76.03	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RGH6_RICCO	3.001e-112	74.90	Reticulocalbin-2, putative OS=Ricinus communis GN=...	[more]
B9HGD1_POPTR	5.660e-111	70.92	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TXZ7_VITVI	1.763e-104	70.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7M1F9_ARALY	1.825e-101	63.60	Calcium-binding EF hand family protein OS=Arabidop...	[more]
Q9FNN0_ARATH	4.065e-101	63.60	Genomic DNA, chromosome 5, P1 clone:MAH20 OS=Arabi...	[more]
C5XYF3_SORBI	6.764e-72	54.80	Putative uncharacterized protein Sb04g007870 OS=So...	[more]
B8AE92_ORYSI	1.507e-71	53.54	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B4FVI6_MAIZE	1.968e-71	54.00	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q0E2Q0_ORYSJ	3.357e-71	53.15	Os02g0221800 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of GT620501 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CALU_RABIT	3.297e-12	28.17	Calumenin OS=Oryctolagus cuniculus GN=CALU PE=1 SV...	[more]
CALU_PONAB	1.636e-11	27.70	Calumenin OS=Pongo abelii GN=CALU PE=2 SV=1	[more]
CALU_HUMAN	2.137e-11	27.70	Calumenin OS=Homo sapiens GN=CALU PE=1 SV=2	[more]
CALU_MOUSE	2.791e-11	28.57	Calumenin OS=Mus musculus GN=Calu PE=1 SV=1	[more]
CALUB_SALSA	6.218e-11	26.18	Calumenin-B OS=Salmo salar GN=calub PE=2 SV=1	[more]
CALUB_DANRE	1.385e-10	26.34	Calumenin-B OS=Danio rerio GN=calub PE=2 SV=1	[more]
CALU_MESAU	2.363e-10	28.08	Calumenin OS=Mesocricetus auratus GN=CALU PE=2 SV=...	[more]
CALU_XENTR	3.086e-10	26.76	Calumenin OS=Xenopus tropicalis GN=calu PE=2 SV=1	[more]
CALU_BOVIN	5.264e-10	25.35	Calumenin OS=Bos taurus GN=CALU PE=2 SV=1	[more]
CALU_XENLA	6.874e-10	26.42	Calumenin OS=Xenopus laevis GN=calu PE=2 SV=1	[more]

BLAST of GT620501 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G08580.1	2.586e-103	63.60	\| Symbols: \| Calcium-binding EF hand family prote...	[more]
AT4G27790.1	3.014e-35	33.33	\| Symbols: \| Calcium-binding EF hand family prote...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 16..40 score
None	No IPR available	SEG	seg	seg	coord: 2..17 score: NAcoord: 21..44 score
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 1..249 score: 2.6
None	No IPR available	PANTHER	PTHR10827:SF13	RETICULOCALBIN	coord: 1..249 score: 2.6
None	No IPR available	SEG	seg	seg	coord: 2..15 score
None	No IPR available	SUPERFAMILY	47473	EF-hand	coord: 90..240 score: 2.7
None	No IPR available	SEG	seg	seg	coord: 34..58 score
None	No IPR available	SEG	seg	seg	coord: 78..89 score: NAcoord: 140..154 score
IPR011992	EF-hand-like domain	GENE3D	1.10.238.10	no description	coord: 94..237 score: 9.9
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152 score: NAcoord: 182..194 score: NAcoord: 221..233 score
IPR018249	EF-HAND 2	PROFILE	PS50222	EF_HAND_2	coord: 127..152 score: 5.782coord: 175..204 score: 9.743coord: 221..243 score: 7

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620501 ID=GT620501; Name=GT620501; organism=Lens culinaris; type=EST; length=797bp
CCAACCACCACTCTTCCCACCGTCACCGCCGTCTCAAACTCCGTTCCAAC
TTCACTCTCTCCCCTTCCCATTCCCACCACCAACCCGTCCCTTTCGACCC
TCTCGTCGCCGAACTCGAACGCAACCGCGAAGACAAGCAATGGGAGAAAC
AGCGCATTGAACATTCTCACCTCGAACTCCTCCATGACTCCGCCCCCGCT
CACGAGTCGCAGCCTGAATGGGAAGACTTCATGAACGCCGAAGACTTCAT
CAATGATGAAGATAAGTTCAATGTTACTTCTCGCCTTTTGATTCTCTTCC
CCAAAATTGATGTTGATCCTGTTGATTCGTTTATCGATGTTCATGAGTTG
ACTCAGTGGAATCTTCAACAGGCTCATAGAGAGGTTTTGCATCGTTCTCA
GAGAGAGATGGAACTTCATGATAAGAATCATGACGGTTTCGTTTCCTTCT
CTGAGTATGATCCTCCTAGTTGGGTTAAATCCGCAGATAAAGATTCCTTT
GGTTATGATATGGGTTGGTGGAAAGAAGAGCATTTTAATGCATCTGATGC
AGATGGAGATGGTGTTCTCAACTTAACTGAGTTCAATGACTTTCTGCACC
CGGCTGATAGTAATAATACGAAGCTTCAACAATGGTTGTGCAAGGAGGAA
GTCAGAGAAAGAGACTCAGATAGAGATGGGAAGGTCAACTTTAAAGAATT
TTTTCACGGGCTCTTTGATTTGGTAAGAAACTATGATGAAGAAGAAACTC
ACACTGATTCCCATCATTCTGATAATTCAATGGATGCTCCAGCTAGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR018249	EF-HAND 2

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