GT622514

EST Overview


Name	GT622514
Unique Name	GT622514
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	691

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT622514 vs. TrEMBL
Match: B9HIA6_POPTR (Integrin-linked protein kinase OS=Populus trichocarpa GN=POPTRDRAFT_765778 PE=3 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 8.743e-64
Identity = 113/138 (81.88%), Postives = 130/138 (94.20%), Query Frame = -2

Query:  280 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGY-IPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLF 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVFKHR+Y+KKVDV+SFAMILYEMLEGEPPF+++EPY+ AK+ AEGHRP FR+KG+ + EL+ELT QCWAADMN+RP F+EILKRLEKIKENLP+DHHWH+F
Sbjct:  301 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPPFSNFEPYEAAKYVAEGHRPTFRSKGFNVFELRELTDQCWAADMNRRPTFLEILKRLEKIKENLPTDHHWHIF 438

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BLAST of GT622514 vs. TrEMBL
Match: D7TX21_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_182.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00003214001 PE=4 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 1.948e-63
Identity = 112/139 (80.58%), Postives = 128/139 (92.09%), Query Frame = -2

Query:  274 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLFAS 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVFKHR+Y+KKVDV+SFAMILYEMLEG+PP ++YEPY+ AK+ AEG RP+FRAKGYI EL+ELT+QCWAADMN RP F+EILKRLEKIKE LP DHHW++F +
Sbjct:  297 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGDPPLSNYEPYEAAKYVAEGQRPMFRAKGYITELKELTEQCWAADMNHRPSFLEILKRLEKIKEILPPDHHWNIFTA 435

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BLAST of GT622514 vs. TrEMBL
Match: Q9XI87_ARATH (Integrin-linked protein kinase OS=Arabidopsis thaliana GN=At1g14000 PE=2 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 5.667e-63
Identity = 112/139 (80.58%), Postives = 126/139 (90.65%), Query Frame = -2

Query:  274 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLFAS 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVFKHRRY+KKVDV+SFAMILYEMLEGEPPFA++EPY+ AKH ++GHRP FR+KG  P+L+EL  +CW ADMNQRP F++ILKRLEKIKE LPSDHHW LF S
Sbjct:  300 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRRYDKKVDVFSFAMILYEMLEGEPPFANHEPYEAAKHVSDGHRPTFRSKGCTPDLRELIVKCWDADMNQRPSFLDILKRLEKIKETLPSDHHWGLFTS 438

[Close]

BLAST of GT622514 vs. TrEMBL
Match: Q9LMF8_ARATH (Integrin-linked protein kinase OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 5.667e-63
Identity = 112/139 (80.58%), Postives = 126/139 (90.65%), Query Frame = -2

Query:  274 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLFAS 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVFKHRRY+KKVDV+SFAMILYEMLEGEPPFA++EPY+ AKH ++GHRP FR+KG  P+L+EL  +CW ADMNQRP F++ILKRLEKIKE LPSDHHW LF S
Sbjct:  307 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRRYDKKVDVFSFAMILYEMLEGEPPFANHEPYEAAKHVSDGHRPTFRSKGCTPDLRELIVKCWDADMNQRPSFLDILKRLEKIKETLPSDHHWGLFTS 445

[Close]

BLAST of GT622514 vs. TrEMBL
Match: Q8S9J9_ARATH (Integrin-linked protein kinase OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 5.667e-63
Identity = 112/139 (80.58%), Postives = 126/139 (90.65%), Query Frame = -2

Query:  274 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLFAS 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVFKHRRY+KKVDV+SFAMILYEMLEGEPPFA++EPY+ AKH ++GHRP FR+KG  P+L+EL  +CW ADMNQRP F++ILKRLEKIKE LPSDHHW LF S
Sbjct:  300 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRRYDKKVDVFSFAMILYEMLEGEPPFANHEPYEAAKHVSDGHRPTFRSKGCTPDLRELIVKCWDADMNQRPSFLDILKRLEKIKETLPSDHHWGLFTS 438

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BLAST of GT622514 vs. TrEMBL
Match: D7KBF8_ARALY (Kinase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_471561 PE=4 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.262e-62
Identity = 111/139 (79.86%), Postives = 126/139 (90.65%), Query Frame = -2

Query:  274 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLFAS 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVF+HRRY+KKVDV+SFAMILYEMLEGEPPFA++EPY+ AKH ++GHRP FR+KG  P+L+EL  +CW ADMNQRP F++ILKRLEKIKE LPSDHHW LF S
Sbjct:  300 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFRHRRYDKKVDVFSFAMILYEMLEGEPPFANHEPYEAAKHVSDGHRPTFRSKGCTPDLRELIVKCWDADMNQRPSFLDILKRLEKIKETLPSDHHWGLFTS 438

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BLAST of GT622514 vs. TrEMBL
Match: B9S913_RICCO (Integrin-linked protein kinase OS=Ricinus communis GN=RCOM_0837790 PE=3 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.262e-62
Identity = 113/137 (82.48%), Postives = 124/137 (90.51%), Query Frame = -2

Query:  280 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLF 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVFKHR+Y+KKVDV+SFAMILYEMLEGEPP A+YEPY+ AK  AEGHRP F AKG+  EL+ELT QCWAADMN+RP F+EILKRLEKIKE LP+DHHW LF
Sbjct:  293 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPPLANYEPYEAAKFVAEGHRPTFHAKGFTIELRELTDQCWAADMNRRPSFLEILKRLEKIKEVLPADHHWSLF 429

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BLAST of GT622514 vs. TrEMBL
Match: Q7XI53_ORYSJ (Integrin-linked protein kinase OS=Oryza sativa subsp. japonica GN=P0519E12.122 PE=3 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 5.137e-56
Identity = 101/139 (72.66%), Postives = 122/139 (87.77%), Query Frame = -2

Query:  277 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPS-DHHWHLFA 690
            HLKVGDFGLSK+I  Q ++DVYKMTGETGSYRYMAPEVFKHR+Y+KKVDV+SFAMILYEMLEG+PPF++YEPY+ AK+  EGHRP FR+KG+  EL+EL + CW+ D++ RP F+EILKRLEKIKE L + DHHWHLF+
Sbjct:  331 HLKVGDFGLSKIIKAQHANDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGDPPFSNYEPYEAAKYVGEGHRPPFRSKGFTNELKELIELCWSGDIHLRPSFLEILKRLEKIKEYLAAHDHHWHLFS 469

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BLAST of GT622514 vs. TrEMBL
Match: B9FUE6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_25251 PE=4 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 5.137e-56
Identity = 101/139 (72.66%), Postives = 122/139 (87.77%), Query Frame = -2

Query:  277 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPS-DHHWHLFA 690
            HLKVGDFGLSK+I  Q ++DVYKMTGETGSYRYMAPEVFKHR+Y+KKVDV+SFAMILYEMLEG+PPF++YEPY+ AK+  EGHRP FR+KG+  EL+EL + CW+ D++ RP F+EILKRLEKIKE L + DHHWHLF+
Sbjct:  256 HLKVGDFGLSKIIKAQHANDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGDPPFSNYEPYEAAKYVGEGHRPPFRSKGFTNELKELIELCWSGDIHLRPSFLEILKRLEKIKEYLAAHDHHWHLFS 394

[Close]

BLAST of GT622514 vs. TrEMBL
Match: B8B4X3_ORYSI (Integrin-linked protein kinase OS=Oryza sativa subsp. indica GN=OsI_26985 PE=3 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 5.137e-56
Identity = 101/139 (72.66%), Postives = 122/139 (87.77%), Query Frame = -2

Query:  277 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPS-DHHWHLFA 690
            HLKVGDFGLSK+I  Q ++DVYKMTGETGSYRYMAPEVFKHR+Y+KKVDV+SFAMILYEMLEG+PPF++YEPY+ AK+  EGHRP FR+KG+  EL+EL + CW+ D++ RP F+EILKRLEKIKE L + DHHWHLF+
Sbjct:  316 HLKVGDFGLSKIIKAQHANDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGDPPFSNYEPYEAAKYVGEGHRPPFRSKGFTNELKELIELCWSGDIHLRPSFLEILKRLEKIKEYLAAHDHHWHLFS 454

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BLAST of GT622514 vs. SwissProt
Match: HT1_ARATH (Serine/threonine-protein kinase HT1 OS=Arabidopsis thaliana GN=HT1 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.125e-15
Identity = 53/137 (38.69%), Postives = 72/137 (52.55%), Query Frame = -2

Query:  292 LKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAE-GHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEK----IKENLPSDHH 687
            +KV DFG S L T        +  G  G+YR+MAPE+ K + Y +KVDVYSF ++L+E+     PF    P   A   AE   RP   A    P L  L ++CW+ + ++RP F  I+  LEK    +KE LP   H
Sbjct:  226 VKVADFGTSCLET-----QCREAKGNMGTYRWMAPEMIKEKPYTRKVDVYSFGIVLWELTTALLPFQGMTPVQAAFAVAEKNERPPLPASCQ-PALAHLIKRCWSENPSKRPDFSNIVAVLEKYDECVKEGLPLTSH 356

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BLAST of GT622514 vs. SwissProt
Match: ROCO4_DICDI (Probable serine/threonine-protein kinase roco4 OS=Dictyostelium discoideum GN=roco4 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 9.522e-15
Identity = 52/129 (40.31%), Postives = 71/129 (55.04%), Query Frame = -2

Query:  316 KVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVF--KHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKH----AAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIK 684
            KV DFGLS+    QS H V   +G  G++++MAPE    +   Y +K D YSFAMILY +L GE PF  Y  Y   K       EG RP    +   P L+ + + CW+ D  +RPHF  I+K L +++
Sbjct: 1174 KVADFGLSQ----QSVHSV---SGLLGNFQWMAPETIGAEEESYTEKADTYSFAMILYTILTGEGPFDEYS-YGKIKFINMIREEGLRPTI-PEDCPPRLRNVIELCWSGDPKKRPHFSYIVKELSELR 1293

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BLAST of GT622514 vs. SwissProt
Match: PYK3_DICDI (Dual specificity protein kinase pyk3 OS=Dictyostelium discoideum GN=pyk3 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.624e-14
Identity = 52/154 (33.77%), Postives = 82/154 (53.25%), Query Frame = -2

Query:  232 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYE-PYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLFAS*IFMSIDMLRNSDD 690
            ++K+ DFGL+  ++     D   ++G T   R+ +PE+ K   Y +KVDVYSF +++YE+  G+ PF   +     AK A E +RP       +  L++L  +CWA+D +QRP F EIL  LE +K           F   +    D+++N DD
Sbjct: 1187 NIKIADFGLATTLS-----DDMTLSGITNP-RWRSPELTKGLVYNEKVDVYSFGLVVYEIYTGKIPFEGLDGTASAAKAAFENYRPAIPPDCPV-SLRKLITKCWASDPSQRPSFTEILTELETMKSK---------FIKQLSFLNDLIQNPDD 1324

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BLAST of GT622514 vs. SwissProt
Match: YTYK2_DICDI (Probable tyrosine-protein kinase DDB_G0283397 OS=Dictyostelium discoideum GN=DDB_G0283397 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 8.061e-14
Identity = 50/126 (39.68%), Postives = 69/126 (54.76%), Query Frame = -2

Query:  310 KVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAA-EGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKEN 684
            K+ DFGLSK    +      +MT   GS  +MAPE FK   Y +KVDVYSFA+IL+E++    P+   EP   A  A+ E +R      G+ P   EL  +CW    + RP F EIL+ L +I+ +
Sbjct:  323 KINDFGLSK----EMKPGPTEMTAAMGSLAWMAPECFKAENYTEKVDVYSFAIILWEIVTCRDPYNGMEPLRLAFLASVEDYR--LPLNGFPPYWVELISKCWNITPSLRPSFKEILQILNQIESD 442

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BLAST of GT622514 vs. SwissProt
Match: SHKE_DICDI (Dual specificity protein kinase shkE OS=Dictyostelium discoideum GN=shkE PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.053e-13
Identity = 45/126 (35.71%), Postives = 69/126 (54.76%), Query Frame = -2

Query:  319 LKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGE---PPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKI 687
            +K+GDFG S++I   ++    K  G  G+  YMAPEV     + +K DVYSF +ILYEM   E   P ++  +P+  A    +   PI     +   L+ L Q+CW  D N+RP F E+ +R+ ++
Sbjct:  373 VKIGDFGFSQVIKTGTTLSDQK--GPKGTALYMAPEVMMKHEFNEKADVYSFGLILYEMATCEELFPEYSEIDPFYDAICNKKLRPPI--PDSFPKSLKTLIQKCWDHDPNKRPSFNEVTQRMNEV 494

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BLAST of GT622514 vs. SwissProt
Match: PYK1_DICDI (Dual specificity protein kinase pyk1 OS=Dictyostelium discoideum GN=splA PE=1 SV=3)

HSP 1 Score: 75.485 bits (184), Expect = 3.063e-13
Identity = 46/125 (36.80%), Postives = 71/125 (56.80%), Query Frame = -2

Query:  313 KVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAA-EGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKE 684
            K+ DFGLS+L   Q+S    +MT   G   YMAPEVFK     +K DVYS+ M+L+E+L  + P    +P   A  AA E +RP         + +E+  QCW ++ + RP F +I+  L+++++
Sbjct: 2271 KISDFGLSRLKMEQAS----QMTQSVGCIPYMAPEVFKGDSNSEKSDVYSYGMVLFELLTSDEPQQDMKPMKMAHLAAYESYRPPIPLT-TSSKWKEILTQCWDSNPDSRPTFKQIIVHLKEMED 2390

[Close]

BLAST of GT622514 vs. SwissProt
Match: ROC11_DICDI (Probable serine/threonine-protein kinase roco11 OS=Dictyostelium discoideum GN=roco11 PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 4.001e-13
Identity = 46/131 (35.11%), Postives = 66/131 (50.38%), Query Frame = -2

Query:  307 KVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVF-KHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDG----AKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENL 684
            KV DFGLS+         +Y ++G  G++++MAPE       Y +K+D YSF+MIL+ +L GE PF  +  +       K   E  RP        P +  L + CW+ D  +RPHF  I+K L     NL
Sbjct: 1333 KVADFGLSQ-------QSLYSVSGLLGNFQWMAPETIGAEESYTEKIDTYSFSMILFTILTGECPFDEFTSFGKMEFIRKIREEDLRPTI-PSDCPPTISNLIELCWSGDPKKRPHFSYIVKELTNFYYNL 1455

[Close]

BLAST of GT622514 vs. SwissProt
Match: PHY1_CERPU (Light-sensor Protein kinase OS=Ceratodon purpureus GN=PHY1 PE=3 SV=3)

HSP 1 Score: 74.7146 bits (182), Expect = 5.225e-13
Identity = 54/141 (38.30%), Postives = 70/141 (49.65%), Query Frame = -2

Query:  316 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVF---------------KHRRYEKKVDVYSFAMILYEMLEGEPPFASYE-PYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIK 690
            H+KV DFGLSK  T  SS      T   G+ R+MAPEV                K  +Y  K DVYSF M+ YE+L G+ PF   + P +  +   EG RP   A   I EL+ L   CW  D  +RP F  I ++L+ +K
Sbjct: 1149 HVKVADFGLSK--TKDSSTRYSNQTWNRGTNRWMAPEVINLGYESTEGEISFDGKVPKYPLKSDVYSFGMVCYEVLTGDVPFPEEKNPNNVKRMVLEGVRPDLPAHCPI-ELKALITDCWNQDPLKRPSFAVICQKLKYLK 1286

[Close]

BLAST of GT622514 vs. SwissProt
Match: Y9963_DICDI (Probable serine/threonine-protein kinase DDB_G0272254 OS=Dictyostelium discoideum GN=DDB_G0272254 PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 6.824e-13
Identity = 43/124 (34.68%), Postives = 68/124 (54.84%), Query Frame = -2

Query:  322 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYD-GAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEK 690
            ++K+ D G+++  +   +     MT   G+  + APE+ +H  Y +K DVYS+A++LYE+L GE P+    P + G   A++G RP        P  ++L   CW+ D N+RP F EI   L K
Sbjct: 1213 NIKIADLGIARETSFTQT-----MT-TIGTVAWTAPEILRHESYNQKADVYSYAIVLYELLTGEEPYQGIPPMNAGILVASKGLRPEL-PDNCDPNWKKLVVWCWSEDPNKRPSFEEITNYLTK 1329

[Close]

BLAST of GT622514 vs. SwissProt
Match: SHKD_DICDI (Dual specificity protein kinase shkD OS=Dictyostelium discoideum GN=shkD PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 6.824e-13
Identity = 48/125 (38.40%), Postives = 67/125 (53.60%), Query Frame = -2

Query:  319 LKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAA--EGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKI 687
            +KV DFG S++   +   D        G+  +MAPEV     Y +K DVYSF +IL+E+L  E P++ ++ YD   +A   E  RP   A   +P L+ L Q CW  +   RP F EIL RL +I
Sbjct:  414 VKVTDFGFSQIKEGEEFQD----KAAKGTPLWMAPEVMMGNPYNEKADVYSFGIILWEILTKEAPYSHHKDYDIFFNAICNEKERPPIPA-DTLPSLRHLIQTCWDHNPQNRPSFSEILFRLNEI 533

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT1G14000.1 (| Symbols: VIK | VH1-interacting kinase | chr1:4797606-4800043 FORWARD LENGTH=438)

HSP 1 Score: 244.588 bits (623), Expect = 3.844e-65
Identity = 112/139 (80.58%), Postives = 126/139 (90.65%), Query Frame = -2

Query:  274 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHLFAS 690
            HLKVGDFGLSKLI VQ+SHDVYKMTGETGSYRYMAPEVFKHRRY+KKVDV+SFAMILYEMLEGEPPFA++EPY+ AKH ++GHRP FR+KG  P+L+EL  +CW ADMNQRP F++ILKRLEKIKE LPSDHHW LF S
Sbjct:  300 HLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRRYDKKVDVFSFAMILYEMLEGEPPFANHEPYEAAKHVSDGHRPTFRSKGCTPDLRELIVKCWDADMNQRPSFLDILKRLEKIKETLPSDHHWGLFTS 438

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT4G18950.1 (| Symbols: | Integrin-linked protein kinase family | chr4:10375685-10378129 FORWARD LENGTH=459)

HSP 1 Score: 112.079 bits (279), Expect = 2.978e-25
Identity = 60/138 (43.48%), Postives = 83/138 (60.14%), Query Frame = -2

Query:  283 HLKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRA--KGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHWHL 690
            HLKV DFG+SKL+TV+        T +  S RY+APEVF    Y+ K DV+SFA+I+ EM+EG  PFA  E  + ++  A  HRP+F+A  K Y   L+ L ++CW     +RP F EI+KRLE I  ++     W +
Sbjct:  294 HLKVADFGVSKLVTVKEDKP---FTCQDISCRYIAPEVFTSEEYDTKADVFSFALIVQEMIEGRMPFAEKEDSEASEAYAGKHRPLFKAPSKNYPHGLKTLIEECWHEKPAKRPTFREIIKRLESILHHMGHKRQWRM 428

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT3G58760.1 (| Symbols: | Integrin-linked protein kinase family | chr3:21728756-21731740 FORWARD LENGTH=471)

HSP 1 Score: 107.457 bits (267), Expect = 7.334e-24
Identity = 61/138 (44.20%), Postives = 81/138 (58.70%), Query Frame = -2

Query:  289 HLKVGDFGLSKLITVQSS--HDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAEGHRPIFRA--KGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHW 690
            HLKV DFG+SKL+ V+ +   D   +T    S+RYMAPEV+++  Y+ KVDV+SFA+IL EM+EG  PF   E  +  K   E  RP F A  K Y   LQEL Q CW  + ++RP F  I+  LE I + +     W
Sbjct:  300 HLKVADFGVSKLLVVKKTVKKDRPVVTCLDSSWRYMAPEVYRNEEYDTKVDVFSFALILQEMIEGCEPFHEIEDREVPKAYIEDERPPFNAPTKSYPFGLQELIQDCWDKEASKRPTFRVIISTLELISDRIARKRSW 437

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT4G35780.1 (| Symbols: | ACT-like protein tyrosine kinase family protein | chr4:16946729-16950405 REVERSE LENGTH=570)

HSP 1 Score: 94.3597 bits (233), Expect = 6.425e-20
Identity = 51/131 (38.93%), Postives = 82/131 (62.60%), Query Frame = -2

Query:  298 LKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAE-GHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSD 687
            +KV DFG+++   VQ+   V  MT ETG+YR+MAPEV +H+ Y+ + DV+S+A++L+E+L GE P++   P   A    + G RP    + + P+L EL ++CW  D   RP+F EI++ L ++   +  D
Sbjct:  427 VKVADFGVAR---VQTESGV--MTAETGTYRWMAPEVIEHKPYDHRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETH-PKLTELLEKCWQQDPALRPNFAEIIEMLNQLIREVGDD 551

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT2G17700.1 (| Symbols: | ACT-like protein tyrosine kinase family protein | chr2:7685778-7689278 REVERSE LENGTH=546)

HSP 1 Score: 93.9745 bits (232), Expect = 8.391e-20
Identity = 52/128 (40.62%), Postives = 81/128 (63.28%), Query Frame = -2

Query:  307 LKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAE-GHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENL 687
            +KV DFG+++   VQ    V  MT ETG+YR+MAPEV +H+ Y  K DV+S+A++L+E+L G+ P+A   P   A    + G RP    K + P+++ L ++CW  D  QRP F EI++ L++I + +
Sbjct:  421 VKVADFGVAR---VQIESGV--MTAETGTYRWMAPEVIEHKPYNHKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTH-PKVKGLLERCWHQDPEQRPLFEEIIEMLQQIMKEV 542

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT4G38470.1 (| Symbols: | ACT-like protein tyrosine kinase family protein | chr4:17999432-18003551 FORWARD LENGTH=575)

HSP 1 Score: 91.2781 bits (225), Expect = 5.439e-19
Identity = 50/128 (39.06%), Postives = 80/128 (62.50%), Query Frame = -2

Query:  307 LKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAAE-GHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENL 687
            +KV DFG+++   V++   V  MT ETG+YR+MAPEV +H+ Y+ K DV+S+ ++L+E+L G+ P+    P   A    + G RP    K   P+L EL ++ W  D  QRP F EI+++L++I + +
Sbjct:  425 VKVADFGVAR---VKAQTGV--MTAETGTYRWMAPEVIEHKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTI-PKNTHPKLAELLERLWEHDSTQRPDFSEIIEQLQEIAKEV 546

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT3G59830.1 (| Symbols: | Integrin-linked protein kinase family | chr3:22103006-22105323 REVERSE LENGTH=477)

HSP 1 Score: 89.3521 bits (220), Expect = 2.067e-18
Identity = 54/140 (38.57%), Postives = 77/140 (55.00%), Query Frame = -2

Query:  289 LKVGDFGLSKLITVQSSHDVYKMTGETG----SYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAA-EGHRPIFR--AKGYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHW 687
            LK+  FGL KL  +    D  K+         S  Y+APE++K   ++K+ DV+SF +ILYE+ EG   F    P + A+    EG RP  R  +K Y PEL+EL ++CW  +++ RP F EI+ RL+KI  N      W
Sbjct:  331 LKISGFGLIKLSKI--GEDSAKVVNHEAQIDKSNYYIAPEIYKDEVFDKRADVHSFGVILYELTEGVSLFHPKPPEEVAESICIEGKRPTIRTKSKSYPPELKELIEECWHPEISVRPIFSEIIIRLDKIVTNCSKQGWW 468

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT2G43850.2 (| Symbols: | Integrin-linked protein kinase family | chr2:18159517-18161984 REVERSE LENGTH=479)

HSP 1 Score: 88.5817 bits (218), Expect = 3.526e-18
Identity = 52/140 (37.14%), Postives = 77/140 (55.00%), Query Frame = -2

Query:  289 LKVGDFGLSKLITVQSSHDVYKMTGETG----SYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAK-HAAEGHRPIFRAK--GYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHW 687
            LK+  FG+ +L  +  S D  K+         S  Y+APEV+K   ++ +VD +SF +ILYE+ EG P F    P + A+    EG RP+F+ K   Y P+++EL ++CW  +   RP F EI+ RL+KI  N      W
Sbjct:  333 LKISGFGMIRLSKI--SQDKAKVANHKAHIDLSNYYIAPEVYKDEIFDLRVDAHSFGVILYEITEGVPVFHPRPPEEVARMMCLEGKRPVFKTKSRSYPPDIKELIEKCWHPEAGIRPTFSEIIIRLDKIVANCSKQGWW 470

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT2G43850.1 (| Symbols: | Integrin-linked protein kinase family | chr2:18159517-18161984 REVERSE LENGTH=479)

HSP 1 Score: 88.5817 bits (218), Expect = 3.526e-18
Identity = 52/140 (37.14%), Postives = 77/140 (55.00%), Query Frame = -2

Query:  289 LKVGDFGLSKLITVQSSHDVYKMTGETG----SYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAK-HAAEGHRPIFRAK--GYIPELQELTQQCWAADMNQRPHFIEILKRLEKIKENLPSDHHW 687
            LK+  FG+ +L  +  S D  K+         S  Y+APEV+K   ++ +VD +SF +ILYE+ EG P F    P + A+    EG RP+F+ K   Y P+++EL ++CW  +   RP F EI+ RL+KI  N      W
Sbjct:  333 LKISGFGMIRLSKI--SQDKAKVANHKAHIDLSNYYIAPEVYKDEIFDLRVDAHSFGVILYEITEGVPVFHPRPPEEVARMMCLEGKRPVFKTKSRSYPPDIKELIEKCWHPEAGIRPTFSEIIIRLDKIVANCSKQGWW 470

[Close]

BLAST of GT622514 vs. TAIR peptide
Match: AT2G24360.1 (| Symbols: | Protein kinase superfamily protein | chr2:10364742-10366075 REVERSE LENGTH=411)

HSP 1 Score: 88.1965 bits (217), Expect = 4.605e-18
Identity = 49/122 (40.16%), Postives = 74/122 (60.66%), Query Frame = -2

Query:  325 LKVGDFGLSKLITVQSSHDVYKMTGETGSYRYMAPEVFKHRRYEKKVDVYSFAMILYEMLEGEPPFASYEPYDGAKHAA-EGHRPIFRAKGYIPELQELTQQCWAADMNQRPHFIEILKRLE 687
            +K+ DFG+++ I VQ+      MT ETG+YR+MAPE+ +HR Y +KVDVYSF ++L+E++ G  PF +      A      G RP       +P L ++  +CW A+   RP F+E++K LE
Sbjct:  270 IKIADFGVAR-IEVQTEG----MTPETGTYRWMAPEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTV-PNDCLPVLSDIMTRCWDANPEVRPCFVEVVKLLE 385

The following BLAST results are available for this feature:

BLAST of GT622514 vs. TrEMBL
BLAST of GT622514 vs. SwissProt
BLAST of GT622514 vs. TAIR peptide

BLAST of GT622514 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HIA6_POPTR	8.743e-64	81.88	Integrin-linked protein kinase OS=Populus trichoca...	[more]
D7TX21_VITVI	1.948e-63	80.58	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9XI87_ARATH	5.667e-63	80.58	Integrin-linked protein kinase OS=Arabidopsis thal...	[more]
Q9LMF8_ARATH	5.667e-63	80.58	Integrin-linked protein kinase OS=Arabidopsis thal...	[more]
Q8S9J9_ARATH	5.667e-63	80.58	Integrin-linked protein kinase OS=Arabidopsis thal...	[more]
D7KBF8_ARALY	1.262e-62	79.86	Kinase family protein OS=Arabidopsis lyrata subsp....	[more]
B9S913_RICCO	1.262e-62	82.48	Integrin-linked protein kinase OS=Ricinus communis...	[more]
Q7XI53_ORYSJ	5.137e-56	72.66	Integrin-linked protein kinase OS=Oryza sativa sub...	[more]
B9FUE6_ORYSJ	5.137e-56	72.66	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8B4X3_ORYSI	5.137e-56	72.66	Integrin-linked protein kinase OS=Oryza sativa sub...	[more]

BLAST of GT622514 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
HT1_ARATH	1.125e-15	38.69	Serine/threonine-protein kinase HT1 OS=Arabidopsis...	[more]
ROCO4_DICDI	9.522e-15	40.31	Probable serine/threonine-protein kinase roco4 OS=...	[more]
PYK3_DICDI	1.624e-14	33.77	Dual specificity protein kinase pyk3 OS=Dictyostel...	[more]
YTYK2_DICDI	8.061e-14	39.68	Probable tyrosine-protein kinase DDB_G0283397 OS=D...	[more]
SHKE_DICDI	1.053e-13	35.71	Dual specificity protein kinase shkE OS=Dictyostel...	[more]
PYK1_DICDI	3.063e-13	36.80	Dual specificity protein kinase pyk1 OS=Dictyostel...	[more]
ROC11_DICDI	4.001e-13	35.11	Probable serine/threonine-protein kinase roco11 OS...	[more]
PHY1_CERPU	5.225e-13	38.30	Light-sensor Protein kinase OS=Ceratodon purpureus...	[more]
Y9963_DICDI	6.824e-13	34.68	Probable serine/threonine-protein kinase DDB_G0272...	[more]
SHKD_DICDI	6.824e-13	38.40	Dual specificity protein kinase shkD OS=Dictyostel...	[more]

BLAST of GT622514 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G14000.1	3.844e-65	80.58	\| Symbols: VIK \| VH1-interacting kinase \| chr1:479...	[more]
AT4G18950.1	2.978e-25	43.48	\| Symbols: \| Integrin-linked protein kinase famil...	[more]
AT3G58760.1	7.334e-24	44.20	\| Symbols: \| Integrin-linked protein kinase famil...	[more]
AT4G35780.1	6.425e-20	38.93	\| Symbols: \| ACT-like protein tyrosine kinase fam...	[more]
AT2G17700.1	8.391e-20	40.63	\| Symbols: \| ACT-like protein tyrosine kinase fam...	[more]
AT4G38470.1	5.439e-19	39.06	\| Symbols: \| ACT-like protein tyrosine kinase fam...	[more]
AT3G59830.1	2.067e-18	38.57	\| Symbols: \| Integrin-linked protein kinase famil...	[more]
AT2G43850.2	3.526e-18	37.14	\| Symbols: \| Integrin-linked protein kinase famil...	[more]
AT2G43850.1	3.526e-18	37.14	\| Symbols: \| Integrin-linked protein kinase famil...	[more]
AT2G24360.1	4.605e-18	40.16	\| Symbols: \| Protein kinase superfamily protein \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 46..61 score
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 1..130 score: 5.2
None	No IPR available	PANTHER	PTHR23257	SERINE-THREONINE PROTEIN KINASE	coord: 1..134 score: 6.1
None	No IPR available	PANTHER	PTHR23257:SF89	ANKYRIN-KINASE	coord: 1..134 score: 6.1
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..135 score: 20
IPR001245	Serine-threonine/tyrosine-protein kinase	PFAM	PF07714	Pkinase_Tyr	coord: 2..121 score: 2.4
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..137 score: 4.3
IPR020635	Tyrosine-protein kinase, catalytic domain	SMART	SM00219	no description	coord: 1..121 score: 0.0

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT622514 ID=GT622514; Name=GT622514; organism=Lens culinaris; type=EST; length=691bp
AACAAAAAGATGAAAGCACAACACACCAGACTGTACGATTTGTCTACTTC
AACTTGTTTGTACACTTAAAACATAATAAACTTGTCCAACAACTTGTGTA
TGTATGCTCATTTGTTGGATATGACACGGGCTTTAATGAGGAAGTAGAAT
TGGAATACGTATTGGTTTTGCAGGTTTTCCAGTTAAGCTTTACCAATCCA
TAAATCTGGAAGCAATGGTAATGGCAAATAAATCATCTGAGTTTCTGAGC
ATATCAATGCTCATAAATATTTATGAAGCAAACAGATGCCAGTGATGATC
TGAAGGTAGATTTTCCTTAATCTTTTCAAGCCGCTTAAGGATCTCTATGA
AATGAGGTCTTTGATTCATATCTGCAGCCCAACACTGCTGCGTTAACTCC
TGCAGCTCAGGGATATAACCCTTCGCTCGAAAAATAGGCCTGTGCCCTTC
AGCTGCGTGTTTAGCTCCATCATAAGGTTCATAACTTGCGAAAGGTGGTT
CACCTTCAAGCATCTCATATAAAATCATTGCAAAGGAGTACACGTCGACC
TTTTTCTCATACCTCCGATGTTTGAACACTTCTGGAGCCATATAGCGATA
GCTCCCAGTTTCACCAGTCATCTTGTATACATCATGAGAACTCTGGACAG
TGATAAGCTTACTCAATCCAAAATCTCCAACTTTTAAATGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR001245	Ser-Thr/Tyr_kinase_cat_dom
IPR011009	Kinase-like_dom_sf
IPR020635	Tyr_kinase_cat_dom

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