GT622547

EST Overview


Name	GT622547
Unique Name	GT622547
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	774

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT622547 vs. TrEMBL
Match: Q8LP21_9SOLA (Flavanone 3-hydroxylase OS=Nierembergia sp. NB17 GN=f3h PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 1.105e-31
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct:  171 KLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

[Close]

BLAST of GT622547 vs. TrEMBL
Match: Q9ZWR0_CITSI (Flavanone 3-hydroxylase OS=Citrus sinensis GN=CitF3H PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.443e-31
Identity = 67/70 (95.71%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQK+VVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct:  170 KLLEVLSEAMGLEKEALTKACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 239

[Close]

BLAST of GT622547 vs. TrEMBL
Match: Q8L8C8_SOLTU (Flavanone 3 beta-hydroxylase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.443e-31
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQKVVVN+YPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct:  169 KLLEVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 238

[Close]

BLAST of GT622547 vs. TrEMBL
Match: Q56I80_9SOLN (Flavanone 3 beta-hydroxylase OS=Solanum pinnatisectum GN=F3H PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.443e-31
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQKVVVN+YPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct:  170 KLLEVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 239

[Close]

BLAST of GT622547 vs. TrEMBL
Match: D3JWB5_CITMA (Flavanone 3-hydroxylase OS=Citrus maxima GN=F3H PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.443e-31
Identity = 67/70 (95.71%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQK+VVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct:  170 KLLEVLSEAMGLEKEALTKACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 239

[Close]

BLAST of GT622547 vs. TrEMBL
Match: D2XQZ1_9MYRT (Flavanone 3-hydroxylase (Fragment) OS=Syzygium malaccense GN=F3H PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.443e-31
Identity = 70/82 (85.37%), Postives = 73/82 (89.02%), Query Frame = 1

Query:  532 QQKQESIFAYV-KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            QQ  E +     K LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  129 QQYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 210

[Close]

BLAST of GT622547 vs. TrEMBL
Match: Q9LEG9_JUGNI (Flavanone 3-hydroxylase (Fragment) OS=Juglans nigra GN=F3H PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.461e-31
Identity = 67/70 (95.71%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  148 KLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 217

[Close]

BLAST of GT622547 vs. TrEMBL
Match: D2DFB1_MEDTR (Flavanone-3-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.461e-31
Identity = 67/82 (81.71%), Postives = 74/82 (90.24%), Query Frame = 1

Query:  529 DQQKQESIFAYVKFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            +Q  ++ +    K LEVLSEAMGLEK+ALTKACVDMDQKVV+NYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct:  159 EQYSEKLMNLACKLLEVLSEAMGLEKDALTKACVDMDQKVVINYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

[Close]

BLAST of GT622547 vs. TrEMBL
Match: C5IUN9_9ROSI (Flavanone 3-hydroxylase (Fragment) OS=Juglans regia GN=F3H PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.461e-31
Identity = 67/70 (95.71%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  148 KLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 217

[Close]

BLAST of GT622547 vs. TrEMBL
Match: A7UNA1_9ROSI (Flavanone-3-hydroxylase (Fragment) OS=Phyllanthus emblica PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.461e-31
Identity = 67/70 (95.71%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  108 KLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 177

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_CALCH (Naringenin,2-oxoglutarate 3-dioxygenase OS=Callistephus chinensis GN=FHT PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.603e-32
Identity = 67/70 (95.71%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  168 KLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 237

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_PETHY (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida GN=AN3 PE=1 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.094e-32
Identity = 67/70 (95.71%), Postives = 69/70 (98.57%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K L+VLSEAMGLEKEALTKACVDMDQKVVVN+YPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct:  173 KLLDVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 242

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_MATIN (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana GN=FHT PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.772e-31
Identity = 64/70 (91.43%), Postives = 68/70 (97.14%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKE+LT ACVDMDQK+VVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  169 KLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 238

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_ARATH (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana GN=F3H PE=1 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.772e-31
Identity = 64/70 (91.43%), Postives = 68/70 (97.14%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKE+LT ACVDMDQK+VVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  170 KLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 239

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_PETCR (Flavanone 3-dioxygenase OS=Petroselinum crispum GN=FHT PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.315e-31
Identity = 64/70 (91.43%), Postives = 68/70 (97.14%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKE LTKACVDMDQKV+VNYYPKCP+P+LTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  171 KLLEVLSEAMGLEKEGLTKACVDMDQKVIVNYYPKCPQPNLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_MALDO (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 3.342e-30
Identity = 63/70 (90.00%), Postives = 67/70 (95.71%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K L VLSEAMGL+ EALTKACVDMDQKVVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  171 KLLGVLSEAMGLDTEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_DIACA (Naringenin,2-oxoglutarate 3-dioxygenase OS=Dianthus caryophyllus GN=FHT PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 3.342e-30
Identity = 63/68 (92.65%), Postives = 66/68 (97.06%), Query Frame = 1

Query:  571 LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            L VLSEAMGLE EALTKACVDMDQK+VVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  173 LGVLSEAMGLELEALTKACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_VITVI (Naringenin,2-oxoglutarate 3-dioxygenase OS=Vitis vinifera GN=F3H PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.659e-29
Identity = 61/70 (87.14%), Postives = 67/70 (95.71%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAM L+K+ALT ACVDMDQKVVVN+YP+CP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  171 KLLEVLSEAMDLDKDALTNACVDMDQKVVVNFYPQCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

[Close]

BLAST of GT622547 vs. SwissProt
Match: FL3H_HORVU (Naringenin,2-oxoglutarate 3-dioxygenase OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 5.336e-28
Identity = 59/66 (89.39%), Postives = 63/66 (95.45%), Query Frame = 1

Query:  577 VLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            VLSEAMGLE EAL KACVDMDQKVVVN+YP+CP+PDLTLGLKRHTDPGTITLLLQD VGGLQAT+D
Sbjct:  177 VLSEAMGLETEALAKACVDMDQKVVVNFYPRCPQPDLTLGLKRHTDPGTITLLLQDLVGGLQATRD 242

[Close]

BLAST of GT622547 vs. SwissProt
Match: FNSI_PETCR (Flavone synthase OS=Petroselinum crispum GN=FNSI PE=1 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 6.970e-28
Identity = 57/70 (81.43%), Postives = 65/70 (92.86%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEK  LTKACVDM+QKV++NYYP CP+PDLTLG++RHTDPGTIT+LLQD VGGLQAT+D
Sbjct:  171 KLLEVLSEAMGLEKGDLTKACVDMEQKVLINYYPTCPQPDLTLGVRRHTDPGTITILLQDMVGGLQATRD 240

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT3G51240.2 (| Symbols: F3H | flavanone 3-hydroxylase | chr3:19025661-19026658 FORWARD LENGTH=274)

HSP 1 Score: 136.346 bits (342), Expect = 1.768e-32
Identity = 64/70 (91.43%), Postives = 68/70 (97.14%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKE+LT ACVDMDQK+VVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:   86 KLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 155

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT3G51240.1 (| Symbols: F3H, TT6, F3'H | flavanone 3-hydroxylase | chr3:19025409-19026658 FORWARD LENGTH=358)

HSP 1 Score: 136.346 bits (342), Expect = 1.768e-32
Identity = 64/70 (91.43%), Postives = 68/70 (97.14%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            K LEVLSEAMGLEKE+LT ACVDMDQK+VVNYYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct:  170 KLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 239

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT4G33640.1 (| Symbols: | unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr4:16159727-16160211 REVERSE LENGTH=95)

HSP 1 Score: 112.079 bits (279), Expect = 3.548e-25
Identity = 54/65 (83.08%), Postives = 58/65 (89.23%), Query Frame = -2

Query:  261 GEVQTDGSYKVTFGTLFHDDRCANIFEALVGTLRAAKKRKVVAYEGELLLQGVHDNVVIILNPSP 455
            G  QTDGSYKVTFG LF+DDRCANIFEALVGTLRAAKKRK+VA+EGELLLQGVHD V I L P+P
Sbjct:   18 GSRQTDGSYKVTFGVLFNDDRCANIFEALVGTLRAAKKRKIVAFEGELLLQGVHDKVEITLRPTP 82

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT4G10500.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:6491089-6492342 FORWARD LENGTH=349)

HSP 1 Score: 77.411 bits (189), Expect = 9.746e-15
Identity = 36/71 (50.70%), Postives = 47/71 (66.20%), Query Frame = 1

Query:  562 VKFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            ++ LE +SE++GLE + ++       Q +  NYYP CPEP+LT GL  H DP  IT+LLQDQV GLQ  KD
Sbjct:  173 LRLLEAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKD 243

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT5G24530.1 (| Symbols: DMR6 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr5:8378964-8383154 FORWARD LENGTH=341)

HSP 1 Score: 75.485 bits (184), Expect = 3.704e-14
Identity = 37/71 (52.11%), Postives = 48/71 (67.61%), Query Frame = 1

Query:  565 KFLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQD-QVGGLQATKD 774
            K  E++SE++GLEK+ + K   +  Q + VNYYP CPEP+LT GL  HTDP  +T+LLQD  V GLQ   D
Sbjct:  165 KIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILID 235

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT4G10490.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:6483900-6485179 FORWARD LENGTH=348)

HSP 1 Score: 74.3294 bits (181), Expect = 8.251e-14
Identity = 33/68 (48.53%), Postives = 46/68 (67.65%), Query Frame = 1

Query:  571 LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            LE +SE++GL K+ ++       Q + +NYYP+CP+P+LT GL  H D   IT+LLQD+V GLQ  KD
Sbjct:  174 LEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKD 241

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT4G16330.2 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:9226298-9227914 REVERSE LENGTH=364)

HSP 1 Score: 73.9442 bits (180), Expect = 1.078e-13
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = 1

Query:  571 LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            L ++SE++GL   ++ +A  ++ Q + V YYP CP+P+LTLGL+ H+D G ITLL+QD V GLQ  KD
Sbjct:  199 LGLISESLGLPCSSIEEAVGEIYQNITVTYYPPCPQPELTLGLQSHSDFGAITLLIQDDVEGLQLYKD 266

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT4G16330.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:9226298-9227235 REVERSE LENGTH=258)

HSP 1 Score: 73.9442 bits (180), Expect = 1.078e-13
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = 1

Query:  571 LEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 774
            L ++SE++GL   ++ +A  ++ Q + V YYP CP+P+LTLGL+ H+D G ITLL+QD V GLQ  KD
Sbjct:   93 LGLISESLGLPCSSIEEAVGEIYQNITVTYYPPCPQPELTLGLQSHSDFGAITLLIQDDVEGLQLYKD 160

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT5G43450.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr5:17457321-17458714 REVERSE LENGTH=362)

HSP 1 Score: 73.1738 bits (178), Expect = 1.838e-13
Identity = 33/63 (52.38%), Postives = 46/63 (73.02%), Query Frame = 1

Query:  574 EVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQ 762
            ++LSEA+GL+ E L +        ++ +YYP CP+PDLTLG+ +HTD   +TLLLQDQ+GGLQ
Sbjct:  191 QLLSEALGLDSETLKRIDCLKGLFMLCHYYPPCPQPDLTLGISKHTDNSFLTLLLQDQIGGLQ 253

[Close]

BLAST of GT622547 vs. TAIR peptide
Match: AT5G43440.2 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr5:17455660-17456608 REVERSE LENGTH=290)

HSP 1 Score: 72.4034 bits (176), Expect = 3.135e-13
Identity = 33/63 (52.38%), Postives = 45/63 (71.43%), Query Frame = 1

Query:  574 EVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQ 762
            ++LSEA+GL+ E L          ++ +YYP CP+PDLTLG+ +HTD   IT+LLQDQ+GGLQ
Sbjct:  194 QLLSEALGLDSEILKNMDCLKGLLMLCHYYPPCPQPDLTLGISKHTDNSFITILLQDQIGGLQ 256

The following BLAST results are available for this feature:

BLAST of GT622547 vs. TrEMBL
BLAST of GT622547 vs. SwissProt
BLAST of GT622547 vs. TAIR peptide

BLAST of GT622547 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8LP21_9SOLA	1.105e-31	97.14	Flavanone 3-hydroxylase OS=Nierembergia sp. NB17 G...	[more]
Q9ZWR0_CITSI	1.443e-31	95.71	Flavanone 3-hydroxylase OS=Citrus sinensis GN=CitF...	[more]
Q8L8C8_SOLTU	1.443e-31	97.14	Flavanone 3 beta-hydroxylase OS=Solanum tuberosum ...	[more]
Q56I80_9SOLN	1.443e-31	97.14	Flavanone 3 beta-hydroxylase OS=Solanum pinnatisec...	[more]
D3JWB5_CITMA	1.443e-31	95.71	Flavanone 3-hydroxylase OS=Citrus maxima GN=F3H PE...	[more]
D2XQZ1_9MYRT	1.443e-31	85.37	Flavanone 3-hydroxylase (Fragment) OS=Syzygium mal...	[more]
Q9LEG9_JUGNI	2.461e-31	95.71	Flavanone 3-hydroxylase (Fragment) OS=Juglans nigr...	[more]
D2DFB1_MEDTR	2.461e-31	81.71	Flavanone-3-hydroxylase OS=Medicago truncatula PE=...	[more]
C5IUN9_9ROSI	2.461e-31	95.71	Flavanone 3-hydroxylase (Fragment) OS=Juglans regi...	[more]
A7UNA1_9ROSI	2.461e-31	95.71	Flavanone-3-hydroxylase (Fragment) OS=Phyllanthus ...	[more]

BLAST of GT622547 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
FL3H_CALCH	1.603e-32	95.71	Naringenin,2-oxoglutarate 3-dioxygenase OS=Callist...	[more]
FL3H_PETHY	2.094e-32	95.71	Naringenin,2-oxoglutarate 3-dioxygenase (Fragment)...	[more]
FL3H_MATIN	1.772e-31	91.43	Naringenin,2-oxoglutarate 3-dioxygenase (Fragment)...	[more]
FL3H_ARATH	1.772e-31	91.43	Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabido...	[more]
FL3H_PETCR	2.315e-31	91.43	Flavanone 3-dioxygenase OS=Petroselinum crispum GN...	[more]
FL3H_MALDO	3.342e-30	90.00	Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus d...	[more]
FL3H_DIACA	3.342e-30	92.65	Naringenin,2-oxoglutarate 3-dioxygenase OS=Dianthu...	[more]
FL3H_VITVI	1.659e-29	87.14	Naringenin,2-oxoglutarate 3-dioxygenase OS=Vitis v...	[more]
FL3H_HORVU	5.336e-28	89.39	Naringenin,2-oxoglutarate 3-dioxygenase OS=Hordeum...	[more]
FNSI_PETCR	6.970e-28	81.43	Flavone synthase OS=Petroselinum crispum GN=FNSI P...	[more]

BLAST of GT622547 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G51240.2	1.768e-32	91.43	\| Symbols: F3H \| flavanone 3-hydroxylase \| chr3:19...	[more]
AT3G51240.1	1.768e-32	91.43	\| Symbols: F3H, TT6, F3'H \| flavanone 3-hydroxylas...	[more]
AT4G33640.1	3.548e-25	83.08	\| Symbols: \| unknown protein; Has 30201 Blast hit...	[more]
AT4G10500.1	9.746e-15	50.70	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT5G24530.1	3.704e-14	52.11	\| Symbols: DMR6 \| 2-oxoglutarate (2OG) and Fe(II)-...	[more]
AT4G10490.1	8.251e-14	48.53	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT4G16330.2	1.078e-13	50.00	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT4G16330.1	1.078e-13	50.00	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT5G43450.1	1.838e-13	52.38	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT5G43440.2	3.135e-13	52.38	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent oxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 65..108 score: 3.4
None	No IPR available	GENE3D	2.60.120.330	no description	coord: 40..109 score: 2
None	No IPR available	PANTHER	PTHR10209	IRON/ASCORBATE-DEPENDENT OXIDOREDUCTASE FAMILY MEMBER	coord: 40..109 score: 2.5
None	No IPR available	PANTHER	PTHR10209:SF16	NARINGENIN,2-OXOGLUTARATE 3-DIOXYGENASE (NARINGENIN 3- DIOXYGENASE) (FLAVANONE 3-HYDROXYLASE)	coord: 40..109 score: 2.5
None	No IPR available	SEG	seg	seg	coord: 1..27 score
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 40..109 score: 1.7
None	No IPR available	SEG	seg	seg	coord: 34..46 score
None	No IPR available	SEG	seg	seg	coord: 1..13 score
None	No IPR available	PANTHER	PTHR22739	STRIATED MUSCLE ACTIVATOR OF RHO-DEPENDENT SIGNALING-RELATED	coord: 16..95 score: 0.0
None	No IPR available	SEG	seg	seg	coord: 11..34 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT622547 ID=GT622547; Name=GT622547; organism=Lens culinaris; type=EST; length=774bp
TTTCAAATAAAAAAAAAGTTAGTAACACAAATATTGGGGTTTTGTTACAC
AAAGTTACCACTCAAATATGTTGGTTTTACAATTGATATACAAACTAAAT
GGTAATGAGTTTATGAAGAGATACATGTACTAGTTCACTTCACAAGCATT
TTTATAAGCCCTTACCCAACATGCATACATTACAAACAATCACACAAAGT
AGAATCATGCGAGCCAAGGAAGATCAATGGTCCTCCGCGATTGTATCAGT
TGACAGCAGCAGGGGAAGGGTTAAGAATGATTACCACATTATCATGGACT
CCCTGCAACAGTAGCTCACCCTCGTATGCAACTACTTTACGCTTTTTGGC
AGCTCGTAGTGTGCCAACAAGGGCTTCAAATATATTTGCGCACCGATCAT
CGTGAAAGAGCGTTCCGAATGTAACCTTGTAAGAACCATCTGTTTGAACC
TCTCCAAGCCTCTTGATTTCTTCCCTGAGTTTTTCGACCTCTTCTTCTAC
ATTCATCATTACAGTATTCTCCCTCTCGGACCAACAAAAACAGGAAAGTA
TATTTGCTTATGTGAAGTTTTTGGAAGTTTTATCAGAGGCTATGGGGTTA
GAGAAAGAAGCTCTAACAAAAGCATGTGTTGATATGGATCAAAAGGTTGT
GGTGAATTACTACCCAAAATGCCCTGAACCTGATCTTACTCTTGGGCTGA
AACGACACACTGACCCTGGCACAATAACTCTGTTGCTTCAAGATCAAGTG
GGTGGTCTTCAAGCTACTAAAGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase

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