GT623254

EST Overview


Name	GT623254
Unique Name	GT623254
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	707

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT623254 vs. TrEMBL
Match: C6TAH5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.359e-43
Identity = 91/108 (84.26%), Postives = 94/108 (87.04%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVWLASSSHVM AEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLW+AQ     +Y       SF+I
Sbjct:   90 FLVFSLKFGVWLASSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWIAQPPENMKYAALVICGSFVI 197

HSP 2 Score: 177.178 bits (448), Expect = 1.150e-42
Identity = 88/98 (89.80%), Postives = 95/98 (96.94%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QPPENM+ AALV+CGSFVIEGASL+VAIQAVK+GAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGL IAGASLVAV+ TGNA+YDPIGSI+VGNLL
Sbjct:  179 QPPENMKYAALVICGSFVIEGASLIVAIQAVKKGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLVIAGASLVAVNVTGNAIYDPIGSIVVGNLL 276

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BLAST of GT623254 vs. TrEMBL
Match: B9HV32_POPTR (Metal tolerance protein (Fragment) OS=Populus trichocarpa GN=PtrMTP7 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 6.312e-41
Identity = 83/108 (76.85%), Postives = 91/108 (84.26%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVW +++SHVM AEVVHS+ADFANQALLAYGLSSSRRAPDA+HPYGYSKERFVWSLISAVGIFCLGSGAT+VNG+QNLW A      +Y       SFII
Sbjct:   30 FLVFSLKFGVWFSTNSHVMLAEVVHSIADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVNGIQNLWTAHPPGNIQYAALVIGGSFII 137

HSP 2 Score: 163.31 bits (412), Expect = 1.719e-38
Identity = 82/97 (84.54%), Postives = 91/97 (93.81%), Query Frame = 3

Query:  417 PPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            PP N+Q AALV+ GSF+IEGASL+VAIQAVK+GAAAEGM +RDYIWRGHDPTSVAVMTEDGAAVTGLAIA ASLVAV+ TGNA+YDPIGSI+VGNLL
Sbjct:  120 PPGNIQYAALVIGGSFIIEGASLIVAIQAVKKGAAAEGMTVRDYIWRGHDPTSVAVMTEDGAAVTGLAIAAASLVAVNTTGNAIYDPIGSIIVGNLL 216

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BLAST of GT623254 vs. TrEMBL
Match: D7KHU0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_892136 PE=4 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 8.244e-41
Identity = 86/108 (79.63%), Postives = 90/108 (83.33%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVW  SSSHV+ AEVVHSVADFANQALLAYGLSSSRRAPDA+HPYGYSKERFVWSLISAVGIFCLGSGAT+VNGVQNLW +      EY       SFII
Sbjct:  121 FLVFSLKFGVWWTSSSHVIMAEVVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVNGVQNLWTSSPPPNMEYAALVIGGSFII 228

HSP 2 Score: 157.918 bits (398), Expect = 7.222e-37
Identity = 79/97 (81.44%), Postives = 87/97 (89.69%), Query Frame = 3

Query:  417 PPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            PP NM+ AALV+ GSF+IEGASLLVAIQ+VK+GAA EGM +RDYIWRGHDPTSVAVMTEDGAAV GLAIA ASLVAV  TGN +YDPIGSI+VGNLL
Sbjct:  211 PPPNMEYAALVIGGSFIIEGASLLVAIQSVKKGAAQEGMTIRDYIWRGHDPTSVAVMTEDGAAVAGLAIAAASLVAVRMTGNPIYDPIGSIVVGNLL 307

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BLAST of GT623254 vs. TrEMBL
Match: B9FEB5_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_14188 PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.837e-40
Identity = 84/108 (77.78%), Postives = 92/108 (85.19%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVWL++SSHVM AE+VHSVADFANQALLAYGLSSSRRAPDA+HPYGYSKERFVWSLISAVGIFCLGSGAT+V+GVQNLW +Q      Y       SF+I
Sbjct:   42 FLVFSLKFGVWLSTSSHVMLAELVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVHGVQNLWNSQPPENIHYAALVIGGSFLI 149

HSP 2 Score: 167.548 bits (423), Expect = 9.115e-40
Identity = 84/98 (85.71%), Postives = 92/98 (93.88%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QPPEN+  AALV+ GSF+IEGASLLVAI+AV++GAAAEGM +RDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAV  TGNAMYDPIGSI+VGNLL
Sbjct:  131 QPPENIHYAALVIGGSFLIEGASLLVAIKAVRKGAAAEGMSIRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVQTTGNAMYDPIGSIIVGNLL 228

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BLAST of GT623254 vs. TrEMBL
Match: B8AS09_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_15280 PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.837e-40
Identity = 84/108 (77.78%), Postives = 92/108 (85.19%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVWL++SSHVM AE+VHSVADFANQALLAYGLSSSRRAPDA+HPYGYSKERFVWSLISAVGIFCLGSGAT+V+GVQNLW +Q      Y       SF+I
Sbjct:  142 FLVFSLKFGVWLSTSSHVMLAELVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVHGVQNLWNSQPPENIHYAALVIGGSFLI 249

HSP 2 Score: 167.548 bits (423), Expect = 9.115e-40
Identity = 84/98 (85.71%), Postives = 92/98 (93.88%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QPPEN+  AALV+ GSF+IEGASLLVAI+AV++GAAAEGM +RDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAV  TGNAMYDPIGSI+VGNLL
Sbjct:  231 QPPENIHYAALVIGGSFLIEGASLLVAIKAVRKGAAAEGMSIRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVQTTGNAMYDPIGSIIVGNLL 328

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BLAST of GT623254 vs. TrEMBL
Match: D7SS51_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_54.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029626001 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 3.133e-40
Identity = 84/108 (77.78%), Postives = 90/108 (83.33%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVWL +SSHVM AEVVHS ADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGAT+V+GVQNLW +      +Y       SF+I
Sbjct:  129 FLVFSLKFGVWLTTSSHVMLAEVVHSAADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATIVHGVQNLWTSHPPANIQYAALVIGGSFLI 236

HSP 2 Score: 160.614 bits (405), Expect = 1.114e-37
Identity = 80/97 (82.47%), Postives = 90/97 (92.78%), Query Frame = 3

Query:  417 PPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            PP N+Q AALV+ GSF+IEGASL+VAI AVK+GAAAEGMK+RDY+WRGHDPTSVAVMTEDGAAVTGL IAGASLVAV+ TGN +YDPIGSI+VGNLL
Sbjct:  219 PPANIQYAALVIGGSFLIEGASLVVAIHAVKKGAAAEGMKVRDYVWRGHDPTSVAVMTEDGAAVTGLIIAGASLVAVNTTGNPIYDPIGSIVVGNLL 315

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BLAST of GT623254 vs. TrEMBL
Match: Q7XVV0_ORYSA (OSJNBa0035B13.1 protein OS=Oryza sativa GN=OSJNBa0035B13.1 PE=4 SV=3)

HSP 1 Score: 165.622 bits (418), Expect = 3.464e-39
Identity = 83/97 (85.57%), Postives = 91/97 (93.81%), Query Frame = 3

Query:  417 PPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            PPEN+  AALV+ GSF+IEGASLLVAI+AV++GAAAEGM +RDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAV  TGNAMYDPIGSI+VGNLL
Sbjct:   94 PPENIHYAALVIGGSFLIEGASLLVAIKAVRKGAAAEGMSIRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVQTTGNAMYDPIGSIIVGNLL 190

HSP 2 Score: 61.6178 bits (148), Expect = 7.044e-8
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQAL 111
            FLVFSLK GVWL++SSHVM AE+VHSVADFANQ L
Sbjct:   42 FLVFSLKFGVWLSTSSHVMLAELVHSVADFANQVL 76

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BLAST of GT623254 vs. TrEMBL
Match: B9RKV4_RICCO (Metal tolerance protein C4, putative OS=Ricinus communis GN=RCOM_1562020 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 2.245e-38
Identity = 81/97 (83.51%), Postives = 92/97 (94.85%), Query Frame = 3

Query:  417 PPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            PPEN++ AALV+ GSF+IEGASL+VAIQAVK+GAAAEGMK+RDY+WRGHDPTSVAVMTEDGAAVTGLAIA ASLVAV+ TGNA+YD IGSI+VGNLL
Sbjct:   51 PPENIKYAALVIGGSFIIEGASLVVAIQAVKKGAAAEGMKVRDYVWRGHDPTSVAVMTEDGAAVTGLAIAAASLVAVNTTGNAIYDSIGSIIVGNLL 147

HSP 2 Score: 78.9518 bits (193), Expect = 4.264e-13
Identity = 38/56 (67.86%), Postives = 42/56 (75.00%), Query Frame = 1

Query:  163 YGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            YGYSKERFVWSLISAVGIFCLGSGAT+V+GVQNLW +      +Y       SFII
Sbjct:   13 YGYSKERFVWSLISAVGIFCLGSGATIVHGVQNLWTSHPPENIKYAALVIGGSFII 68

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BLAST of GT623254 vs. TrEMBL
Match: B4KSP8_DROMO (GI18486 OS=Drosophila mojavensis GN=GI18486 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 3.914e-36
Identity = 47/101 (46.53%), Postives = 67/101 (66.34%), Query Frame = 1

Query:   25 KSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFI 327
            K+G WL   SH MFAEV+HS+AD  NQ +LA+G+  S + PD +HPYGY   R+V SLIS VGIFC+GSG ++ +G++ L   +V     ++ C  + S +
Sbjct:  319 KTGGWLYCGSHSMFAEVIHSLADLVNQLILAFGIYKSSQLPDTVHPYGYMNMRYVSSLISGVGIFCVGSGLSIYHGIEGLLNPEVITDLFWVYCILAGSLV 419

HSP 2 Score: 72.7886 bits (177), Expect = 3.914e-36
Identity = 35/87 (40.23%), Postives = 54/87 (62.07%), Query Frame = 3

Query:  447 VLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            +L GS V EGA+L+VAI  +K+ A    +  ++Y+  G DP    V+ ED AAVTG+ +A   +     TG+ ++D IGS+++G LL
Sbjct:  413 ILAGSLVSEGATLVVAINELKRAAKENKLSFKEYVLTGKDPCVNVVLCEDAAAVTGVVVAAGCMGLSSLTGSPIFDAIGSLVIGGLL 499

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BLAST of GT623254 vs. TrEMBL
Match: A9T1R0_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_138991 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 7.474e-34
Identity = 76/121 (62.81%), Postives = 88/121 (72.73%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFIISFCHLSYFNYWSV 369
            F+VFS+K GVW  +SSHVM AE VHS AD ANQ LLAYGL SSRRAPDA+HPYGYS+ERFVWSLISAVGIFCLG GAT+V+GVQNL  A+  +         + S I+    L Y  Y +V
Sbjct:   22 FVVFSMKVGVWFMTSSHVMLAEAVHSAADLANQGLLAYGLISSRRAPDALHPYGYSRERFVWSLISAVGIFCLGCGATIVHGVQNLATAEPLSNVTIAAAVLTGSMIMEGASL-YVAYGAV 141

HSP 2 Score: 127.872 bits (320), Expect = 8.004e-28
Identity = 63/98 (64.29%), Postives = 76/98 (77.55%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            +P  N+ +AA VL GS ++EGASL VA  AVK+GAAAEGM LR+Y+WRGHDPTSVAV+ EDGAA  GL IAG +L A   TGN M+D +GSI+VG LL
Sbjct:  111 EPLSNVTIAAAVLTGSMIMEGASLYVAYGAVKKGAAAEGMSLREYLWRGHDPTSVAVLMEDGAACAGLGIAGIALTAAEITGNPMWDALGSIVVGGLL 208

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BLAST of GT623254 vs. SwissProt
Match: MTPC4_ARATH (Metal tolerance protein C4 OS=Arabidopsis thaliana GN=MTPC4 PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 7.967e-41
Identity = 84/108 (77.78%), Postives = 89/108 (82.41%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVW  SSSHV+ AEVVHSVADFANQALLAYGLSSSRRAPDA+HPYGYSKERFVWSLISAVGIFCLGSGAT+VNGVQNLW +      E        SF+I
Sbjct:  125 FLVFSLKFGVWWTSSSHVIMAEVVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVNGVQNLWTSSPPPNMELAAVVIGGSFLI 232

HSP 2 Score: 157.918 bits (398), Expect = 4.833e-38
Identity = 79/97 (81.44%), Postives = 88/97 (90.72%), Query Frame = 3

Query:  417 PPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            PP NM+LAA+V+ GSF+IEGASLLVAIQ+VK+GAA EGM +RDYIWRGHDPTSVAVMTEDGAAV GLAIA ASLVAV  TGN +YDPIGSI+VGNLL
Sbjct:  215 PPPNMELAAVVIGGSFLIEGASLLVAIQSVKKGAAQEGMTIRDYIWRGHDPTSVAVMTEDGAAVAGLAIAAASLVAVRMTGNPIYDPIGSIVVGNLL 311

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BLAST of GT623254 vs. SwissProt
Match: ZNT9_DANRE (Zinc transporter 9 OS=Danio rerio GN=slc30a9 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 9.477e-18
Identity = 47/106 (44.34%), Postives = 64/106 (60.38%), Query Frame = 1

Query:   10 LVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFI 327
            L F  K   W+ + S  MF+E +HS+AD  NQALLA G+S S R PDAIHPYG+S  R++ SLIS VGIF +G+G +  +G+  L   Q      +  C  + S +
Sbjct:  254 LNFFFKLLAWVYTGSASMFSEAIHSLADTCNQALLALGISQSVRNPDAIHPYGFSNMRYIASLISGVGIFMMGAGLSWYHGIMGLLHPQPIESLLWAYCILAGSLV 359

HSP 2 Score: 82.4185 bits (202), Expect = 2.581e-15
Identity = 41/98 (41.84%), Postives = 60/98 (61.22%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QP E++  A  +L GS V EGA+LLVAI  +K+ A  +G+   +Y+ +  DP++  V+ ED AAV G+ +A   +     TGN  YD +GS+ VG LL
Sbjct:  342 QPIESLLWAYCILAGSLVSEGATLLVAINEIKKSARTQGLSFYEYVMQSRDPSTNVVLLEDAAAVLGVVLAAGCMGLTSLTGNPYYDSLGSLGVGTLL 439

[Close]

BLAST of GT623254 vs. SwissProt
Match: ZNT9_XENLA (Zinc transporter 9 OS=Xenopus laevis GN=slc30a9 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.158e-15
Identity = 44/106 (41.51%), Postives = 62/106 (58.49%), Query Frame = 1

Query:   10 LVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFI 327
            L F  K   W+ + S  MF+E +HS+AD  NQALLA G+S S R PD  HPYG++  R++ SLIS VGIF +G+G +  +G+  L   Q      +  C  + S +
Sbjct:  240 LNFFFKLLAWVYTGSASMFSEALHSLADTLNQALLALGISQSARTPDPGHPYGFTNMRYIASLISGVGIFMMGAGLSWYHGIIGLLHPQPIESLLWAYCILAGSLV 345

HSP 2 Score: 82.8037 bits (203), Expect = 1.976e-15
Identity = 41/98 (41.84%), Postives = 62/98 (63.27%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QP E++  A  +L GS V EGA+LLVAI  +++ + A+G+    Y+ +  DP++  V+ ED AAV GL +A + +     TGN +YD +GS+ VG LL
Sbjct:  328 QPIESLLWAYCILAGSLVSEGATLLVAINEIRKSSRAKGLSFYQYVMQSRDPSTNVVLMEDAAAVLGLVMAASCMGLTSLTGNPLYDSLGSLGVGTLL 425

[Close]

BLAST of GT623254 vs. SwissProt
Match: ZNT9_PONAB (Zinc transporter 9 OS=Pongo abelii GN=SLC30A9 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.371e-15
Identity = 40/97 (41.24%), Postives = 58/97 (59.79%), Query Frame = 1

Query:   37 WLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFI 327
            W+ + S  MF+E +HS++D  NQ LLA G+S S + PD  HPYG+S  R++ SLIS VGIF +G+G +  +GV  L   Q      +  C  + S +
Sbjct:  259 WIYTGSASMFSEAIHSLSDTCNQGLLALGISKSVQTPDPSHPYGFSNMRYISSLISGVGIFMMGAGLSWYHGVMGLLHPQPIESLLWAYCILAGSLV 355

HSP 2 Score: 81.2629 bits (199), Expect = 5.749e-15
Identity = 41/98 (41.84%), Postives = 60/98 (61.22%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QP E++  A  +L GS V EGA+LLVA+  +++ A A+GM    Y+    DP++  ++ ED AAV G+ IA   +     TGN +YD +GS+ VG LL
Sbjct:  338 QPIESLLWAYCILAGSLVSEGATLLVAVNELRRNARAKGMSFYKYVMESRDPSTNVILLEDTAAVLGVIIAATCMGLTSITGNPLYDSLGSLGVGTLL 435

[Close]

BLAST of GT623254 vs. SwissProt
Match: ZNT9_HUMAN (Zinc transporter 9 OS=Homo sapiens GN=SLC30A9 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.371e-15
Identity = 40/97 (41.24%), Postives = 58/97 (59.79%), Query Frame = 1

Query:   37 WLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFI 327
            W+ + S  MF+E +HS++D  NQ LLA G+S S + PD  HPYG+S  R++ SLIS VGIF +G+G +  +GV  L   Q      +  C  + S +
Sbjct:  258 WIYTGSASMFSEAIHSLSDTCNQGLLALGISKSVQTPDPSHPYGFSNMRYISSLISGVGIFMMGAGLSWYHGVMGLLHPQPIESLLWAYCILAGSLV 354

HSP 2 Score: 81.2629 bits (199), Expect = 5.749e-15
Identity = 41/98 (41.84%), Postives = 60/98 (61.22%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QP E++  A  +L GS V EGA+LLVA+  +++ A A+GM    Y+    DP++  ++ ED AAV G+ IA   +     TGN +YD +GS+ VG LL
Sbjct:  337 QPIESLLWAYCILAGSLVSEGATLLVAVNELRRNARAKGMSFYKYVMESRDPSTNVILLEDTAAVLGVIIAATCMGLTSITGNPLYDSLGSLGVGTLL 434

[Close]

BLAST of GT623254 vs. SwissProt
Match: ZNT9_MOUSE (Zinc transporter 9 OS=Mus musculus GN=Slc30a9 PE=1 SV=2)

HSP 1 Score: 81.6481 bits (200), Expect = 4.402e-15
Identity = 39/97 (40.21%), Postives = 58/97 (59.79%), Query Frame = 1

Query:   37 WLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFI 327
            W+ + S  MF+E +HS++D  NQ LLA G+S S + PD  HPYG+S  R++ SLIS VGIF +G+G +  +G+  L   Q      +  C  + S +
Sbjct:  257 WIYTGSASMFSEAIHSLSDTCNQGLLALGISKSVQTPDPSHPYGFSNMRYISSLISGVGIFMMGAGLSWYHGIMGLLHPQPMESLLWAYCILAGSLV 353

HSP 2 Score: 79.337 bits (194), Expect = 2.185e-14
Identity = 41/98 (41.84%), Postives = 59/98 (60.20%), Query Frame = 3

Query:  414 QPPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            QP E++  A  +L GS V EGA+LLVAI  +++ A A+G     Y+    DP++  ++ ED AAV G+ IA   +     TGN +YD +GS+ VG LL
Sbjct:  336 QPMESLLWAYCILAGSLVSEGATLLVAINELRRSAQAKGTTFYKYVMESRDPSTNVILLEDTAAVLGVIIAATCMGLTSITGNPLYDSLGSLGVGTLL 433

[Close]

BLAST of GT623254 vs. TAIR peptide
Match: AT1G51610.1 (| Symbols: | Cation efflux family protein | chr1:19136625-19139621 FORWARD LENGTH=457)

HSP 1 Score: 167.162 bits (422), Expect = 8.093e-42
Identity = 84/108 (77.78%), Postives = 89/108 (82.41%), Query Frame = 1

Query:    7 FLVFSLKSGVWLASSSHVMFAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISAVGIFCLGSGATVVNGVQNLWVAQVFNQTEYLKCRHSNSFII 330
            FLVFSLK GVW  SSSHV+ AEVVHSVADFANQALLAYGLSSSRRAPDA+HPYGYSKERFVWSLISAVGIFCLGSGAT+VNGVQNLW +      E        SF+I
Sbjct:  125 FLVFSLKFGVWWTSSSHVIMAEVVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVNGVQNLWTSSPPPNMELAAVVIGGSFLI 232

HSP 2 Score: 157.918 bits (398), Expect = 4.910e-39
Identity = 79/97 (81.44%), Postives = 88/97 (90.72%), Query Frame = 3

Query:  417 PPENMQLAALVLCGSFVIEGASLLVAIQAVKQGAAAEGMKLRDYIWRGHDPTSVAVMTEDGAAVTGLAIAGASLVAVHYTGNAMYDPIGSILVGNLL 707
            PP NM+LAA+V+ GSF+IEGASLLVAIQ+VK+GAA EGM +RDYIWRGHDPTSVAVMTEDGAAV GLAIA ASLVAV  TGN +YDPIGSI+VGNLL
Sbjct:  215 PPPNMELAAVVIGGSFLIEGASLLVAIQSVKKGAAQEGMTIRDYIWRGHDPTSVAVMTEDGAAVAGLAIAAASLVAVRMTGNPIYDPIGSIVVGNLL 311

The following BLAST results are available for this feature:

BLAST of GT623254 vs. TrEMBL
BLAST of GT623254 vs. SwissProt
BLAST of GT623254 vs. TAIR peptide

BLAST of GT623254 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TAH5_SOYBN	1.359e-43	84.26	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9HV32_POPTR	6.312e-41	76.85	Metal tolerance protein (Fragment) OS=Populus tric...	[more]
D7KHU0_ARALY	8.244e-41	79.63	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9FEB5_ORYSJ	1.837e-40	77.78	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AS09_ORYSI	1.837e-40	77.78	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7SS51_VITVI	3.133e-40	77.78	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q7XVV0_ORYSA	3.464e-39	85.57	OSJNBa0035B13.1 protein OS=Oryza sativa GN=OSJNBa0...	[more]
B9RKV4_RICCO	2.245e-38	83.51	Metal tolerance protein C4, putative OS=Ricinus co...	[more]
B4KSP8_DROMO	3.914e-36	46.53	GI18486 OS=Drosophila mojavensis GN=GI18486 PE=4 S...	[more]
A9T1R0_PHYPA	7.474e-34	62.81	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of GT623254 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
MTPC4_ARATH	7.967e-41	77.78	Metal tolerance protein C4 OS=Arabidopsis thaliana...	[more]
ZNT9_DANRE	9.477e-18	44.34	Zinc transporter 9 OS=Danio rerio GN=slc30a9 PE=2 ...	[more]
ZNT9_XENLA	1.158e-15	41.51	Zinc transporter 9 OS=Xenopus laevis GN=slc30a9 PE...	[more]
ZNT9_PONAB	3.371e-15	41.24	Zinc transporter 9 OS=Pongo abelii GN=SLC30A9 PE=2...	[more]
ZNT9_HUMAN	3.371e-15	41.24	Zinc transporter 9 OS=Homo sapiens GN=SLC30A9 PE=1...	[more]
ZNT9_MOUSE	4.402e-15	40.21	Zinc transporter 9 OS=Mus musculus GN=Slc30a9 PE=1...	[more]

BLAST of GT623254 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G51610.1	8.093e-42	77.78	\| Symbols: \| Cation efflux family protein \| chr1:...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002524	Cation efflux protein	PFAM	PF01545	Cation_efflux	coord: 2..103 score: 2.5
None	No IPR available	GENE3D	1.20.1510.10	no description	coord: 2..99 score: 2.3
None	No IPR available	PANTHER	PTHR13414	HUEL-CATION TRANSPORTER	coord: 3..89 score: 6.7
None	No IPR available	PANTHER	PTHR13414	HUEL-CATION TRANSPORTER	coord: 5..102 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT623254 ID=GT623254; Name=GT623254; organism=Lens culinaris; type=EST; length=707bp
GTGTACTTCCTTGTTTTTTCACTCAAATCTGGAGTATGGTTAGCTTCCTC
TAGTCATGTTATGTTCGCTGAAGTTGTGCATTCCGTTGCCGATTTTGCAA
ACCAGGCGCTGCTTGCTTATGGTCTATCTAGCTCTAGGCGGGCACCAGAT
GCTATCCATCCGTATGGTTATTCCAAAGAAAGATTTGTTTGGTCATTGAT
ATCTGCTGTTGGGATATTTTGTCTTGGTTCTGGTGCTACGGTTGTTAATG
GAGTTCAAAACTTGTGGGTTGCACAGGTATTCAACCAAACTGAGTATTTG
AAATGCAGACATTCAAATTCTTTCATCATAAGCTTTTGTCATCTTTCTTA
TTTTAATTACTGGTCTGTGAGTTGTCTCTCATTTAATTTATTGGCTTTTG
AGAAATTATTTTTCAGCCCCCTGAAAATATGCAATTAGCAGCTTTGGTGT
TATGTGGTTCATTTGTCATTGAAGGAGCCTCTCTTCTTGTTGCCATTCAA
GCTGTAAAGCAAGGCGCAGCTGCGGAGGGAATGAAGTTAAGAGACTATAT
TTGGCGTGGACATGATCCTACATCTGTTGCAGTAATGACAGAGGATGGTG
CTGCAGTAACTGGCCTTGCTATTGCTGGGGCATCATTGGTGGCAGTGCAC
TATACCGGAAATGCTATGTATGATCCCATAGGCTCAATCTTGGTTGGCAA
CTTACTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006812	monoatomic cation transport
GO:0055085	transmembrane transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0008324	monoatomic cation transmembrane transporter activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR002524	Cation_efflux

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