GT623650

EST Overview


Name	GT623650
Unique Name	GT623650
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	761

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT623650 vs. TrEMBL
Match: A9YWR2_MEDTR (BHLH transcription factor OS=Medicago truncatula PE=4 SV=1)

HSP 1 Score: 322.783 bits (826), Expect = 1.967e-86
Identity = 172/233 (73.82%), Postives = 185/233 (79.40%), Query Frame = 3

Query:   75 MELSQLGFFEELLAPRKDTWSTLSTGLNDLLLPNGWNLDSFDENLLINPSLNPSFASFSTPLDHRFECSYGTDA--VSYPFVDGFTVPELDDSTPLLP-QXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMD-EGTDQINVLGIS 761
            MELSQLGF EELLAPRKDTW+TLSTGLN+LLLPNGW  DSFDENLLINPSLNPSFASFSTPLDHRFEC YGTDA  +SYP++DGF+VPE DDS P+LP Q                EE KISCKVEEQ+SE PVF+MGLC             SKRVEGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERI KLQEE++ EGT+QIN+LGIS
Sbjct:    1 MELSQLGFLEELLAPRKDTWNTLSTGLNELLLPNGWTFDSFDENLLINPSLNPSFASFSTPLDHRFECPYGTDASSLSYPYLDGFSVPEFDDSAPVLPQQESIEEFGFVGSENKRFEESKISCKVEEQVSETPVFNMGLC-------GEKKAKSKRVEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERISKLQEEIEKEGTNQINLLGIS 226

[Close]

BLAST of GT623650 vs. TrEMBL
Match: C6TNX2_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 281.952 bits (720), Expect = 3.848e-74
Identity = 160/244 (65.57%), Postives = 183/244 (75.00%), Query Frame = 3

Query:   75 MELSQLGFFEELLAPRKDT-WST-LSTGLNDLLLPNGWNLDSFDENLLINPSLNPSFASFSTPLDHRFECSYGTDAVSYPFVDGFTVPELD-------DSTPLLPQXXXXXXXXXXXXXXX-----LEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQ-EEMDEGTDQINVLGIS 761
            MELSQLGF +ELLAPRKDT WS  LSTGLN+LLLP+GW+ DSFDEN  +  +LNPSFA+FSTPLDHRFEC YG++A +YPFVDGFT+PELD       +S PLLPQ                    LE+ KI CK+EEQ++EIPVF+MGLC             SK++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQ EE++EGT+QIN+LGIS
Sbjct:    1 MELSQLGFLDELLAPRKDTTWSNALSTGLNELLLPSGWSFDSFDENQGL-ATLNPSFAAFSTPLDHRFECPYGSEA-AYPFVDGFTLPELDSSYTRNDESAPLLPQEDNPSLEDEEFGFLGRDNQSLEQAKIGCKIEEQVTEIPVFNMGLCGEKKPK-------SKKLEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEEIEEGTNQINLLGIS 235

[Close]

BLAST of GT623650 vs. TrEMBL
Match: C6TA88_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 2.581e-70
Identity = 156/244 (63.93%), Postives = 181/244 (74.18%), Query Frame = 3

Query:   75 MELSQLGFFEELLAPRKDT-WST-LSTGLNDLLLPNGWNLDSFDENLLINPSLNPSFASFSTPLDHRFECSYGTDAVSYPFVDGFTVPELD-------DSTPLLPQXXXXXXXXXXXXXXX-----LEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQ-EEMDEGTDQINVLGIS 761
            MELSQLGF +ELLAPRKDT WS+ LSTGLN+LLLP+GW+ DSFDEN  ++ +LNPSFA+FSTPLDHRFEC YG++A +YPFVDGFT+PELD       +S PLLPQ                    LE+ KI CK+EE ++EIP F+MGL              SK++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQ EE +EGT++IN+LGIS
Sbjct:    1 MELSQLGFLDELLAPRKDTSWSSALSTGLNELLLPSGWSFDSFDENQGLS-TLNPSFAAFSTPLDHRFECPYGSEA-AYPFVDGFTLPELDSSYTRNDESAPLLPQEDNPSLEDEEFGFLGSESQSLEQAKIGCKIEE-LTEIPAFNMGL-------GGEKRPKSKKLEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEEKEEGTNRINLLGIS 234

[Close]

BLAST of GT623650 vs. TrEMBL
Match: Q2HRF0_MEDTR (Helix-loop-helix DNA-binding OS=Medicago truncatula GN=MtrDRAFT_AC159144g2v2 PE=4 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 5.791e-46
Identity = 110/187 (58.82%), Postives = 117/187 (62.57%), Query Frame = 3

Query:   87 QLGFFEELLAPRKDTWSTLSTGLNDLLLPNGWNLDSFDENLLINPSLNPSFASFSTPLDHRFECSYGTDAVSYPFVDGFTVPELDDSTPLLP-QXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMD 644
            +LGF EELLAPRKDTW+TLS GLN+LLLPNGW  DSFDENLLINPSLNPSFASFSTPLDHRFE                  PE DDS PLLP Q               LE+ KISCKVEEQ+SE PVF+MGLC             SKRVE +  K           LNDRLSMLRSIVPKISK D
Sbjct:    2 ELGFLEELLAPRKDTWNTLSNGLNELLLPNGWTFDSFDENLLINPSLNPSFASFSTPLDHRFE-----------------FPEFDDSAPLLPQQESIEEFGFVGSENKSLEQSKISCKVEEQVSETPVFNMGLC-------DEKKAKSKRVERRRRKR----------LNDRLSMLRSIVPKISKFD 154

[Close]

BLAST of GT623650 vs. TrEMBL
Match: B9SSC2_RICCO (DNA binding protein, putative OS=Ricinus communis GN=RCOM_0187390 PE=4 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 3.513e-43
Identity = 130/262 (49.62%), Postives = 151/262 (57.63%), Query Frame = 3

Query:   75 MELSQLGFFEELL-APR-KDT-------WSTL-----STGLNDLLLPNGWNLDSFDENLLINPSLNP-SFASFSTPLDHRFECSYGTDAVSYPFVDGFT-----VP-ELDDSTPLLPQXXXXXXXXXXXXXXXL-------EER--KISCKVE-EQMSEI----PVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQ-EEMDEGTDQINVL 752
            MELSQ GF EELL  PR +DT       W+T      +TG ++    NGWN DSFDEN  ++P+ NP SF  F TP +  F+CS+     +YPF DGFT     VP E+DD TP  P                L       EER    SCKVE EQ S       VF MGL              SK++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERI KLQ EE ++GT ++ ++
Sbjct:    1 MELSQHGFLEELLQGPRIRDTNSGSGSSWATFPITTAATGSSEFFT-NGWNFDSFDENTSLSPAPNPCSFLGFPTPTEPTFDCSFNDQ--TYPFFDGFTAATTTVPTEIDDHTPPFPTQEDYPSMVEDEELGGLLLRSHSFEERLSSTSCKVELEQASNNNNNGQVFSMGLSAEKKNK-------SKKLEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERINKLQEEESEDGTTEMTLM 252

[Close]

BLAST of GT623650 vs. TrEMBL
Match: A5BDS2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033510 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 2.784e-40
Identity = 110/245 (44.90%), Postives = 140/245 (57.14%), Query Frame = 3

Query:   75 MELSQLGFFEELLAPRKDTWSTLSTGLNDLLLPNGWNLD-SFDENLLINPSLNPSFASFSTPLDHRFECSYGTDAVSYPFVDGFTVPELDDSTPLLPQXXXXXXXXXXXXXXXLEERK---------------ISCKVEE-QMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTDQINVLGI 758
            ME+++ GF EELLA R D W T+ TG+N+    +GW  D  FDEN  +    N SF  FS P++  F   Y  + +  PF   F+ P++ DS+    Q               +EE +                +CKVE  Q +E PVF++G+C             +K+VEGQPSKNLMAE      LNDRLSMLRS+VPKISKMDRTSILGDTIDYMKELLERI  LQEE +  + Q+N+LGI
Sbjct:    1 MEVNEHGFLEELLALRGDPWETIPTGMNEFF-SHGWGFDYRFDENP-VTFLPNSSFEXFSGPIEPGF--GYSFNEMYSPFGXEFSTPQVTDSS-YTKQDTPPFPTQEDYPFPMMEEEEPAVHPGVDLHNMGLQATCKVEPIQSTEFPVFNVGVCNEVKNKR------TKKVEGQPSKNLMAERRRRKRLNDRLSMLRSVVPKISKMDRTSILGDTIDYMKELLERINNLQEENEVDSSQLNLLGI 234

[Close]

BLAST of GT623650 vs. TrEMBL
Match: B9SMX0_RICCO (DNA binding protein, putative OS=Ricinus communis GN=RCOM_0481980 PE=4 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 1.867e-36
Identity = 108/257 (42.02%), Postives = 141/257 (54.86%), Query Frame = 3

Query:   75 MELS-QLGFFEELLAPRKDTWSTLSTGLNDL--LLPNGWNLDSFDENLLINPSLNPSFA--SF-----STPLDHRFECSYGTDAVSYPF-----VDGFTVPELDDS------TPLLPQXXXXXXXXXXXXXXX---------LEERKISCKVEEQMS-EIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTDQINVL 752
            ME++ Q GF EE L+ R+D   T+ +  +++  +  NGWN   FDE   IN   +  FA  SF     S+PL+H+    Y  + VS PF      D F+ P+  DS      TP  P                         + E + +CK E   S E+PVF +G C             SK++EGQPSKNLMAE      LNDRL+MLRSIVPKISKMDRTSILGDTIDY+KELLERI  LQ+E++ G++Q+N+L
Sbjct:    1 MEINRQQGFLEEFLSLRRDNLETIPSFPSEIGEMFSNGWNFSCFDEFDGINSVASTLFAPNSFYQEFSSSPLEHQDFSHYYFNEVSCPFGDPPFTDDFSAPQFTDSSYNNLDTPPFPVQEGHTPMSLMENDQETGLLPNHVQISEMQAACKFEPSQSPEVPVFSIGACPERKIR-------SKKLEGQPSKNLMAERRRRKRLNDRLAMLRSIVPKISKMDRTSILGDTIDYVKELLERINSLQQELEMGSNQLNIL 250

[Close]

BLAST of GT623650 vs. TrEMBL
Match: A9P9D2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816406 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.871e-28
Identity = 93/256 (36.33%), Postives = 128/256 (50.00%), Query Frame = 3

Query:   75 MELSQLGFFEELLAPRKDTWSTLSTGLNDL--LLPNGWNLDSFDE--NLLINPSLNPSFASFSTPLDHRFECSYGTDAVSYPFVDGFTVPELDDSTPLLPQXXXXXXXXXXXXXXXLEE-----------------------RKISCKVEE-QMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTDQINVLGI 758
            ME++   F EEL+A  +++W       +++  L    +N   F+E    L   S  P     S+PL   F  +Y  + V  PF D F+ P+  D     PQ               +++                        + +CKVE  Q  E+  F+ G+C              K++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERI  LQ+E++ G++++ ++ I
Sbjct:    1 MEINGHDFLEELIALSRESWQPTPNYPSEMNELFSGSFNHGCFEEIPATLPQTSFCPE-GLISSPLKQDFN-NYYFNEVFCPFGDEFSAPQFTDEFSSAPQFTDSSYNTLDTPPFPVQDDTPMSMMEDEELGLLANDQQNLQMQGTCKVEPIQSPEVSAFNAGICPERKIR-------GKKMEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERINSLQQEIEVGSEELKMISI 247

[Close]

BLAST of GT623650 vs. TrEMBL
Match: C6TH74_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 9.288e-28
Identity = 97/242 (40.08%), Postives = 125/242 (51.65%), Query Frame = 3

Query:   75 MELSQLGFFEELLAPRKDTWSTLSTGLNDLLLPNGWNLDSFDENLLINPSLNPSFASFSTPL----DHRFECSYGT--DAVSYPFVDGF---------TVPEL-DDSTPLLPQXXXXXXXXXXXXXXXLEERKISCKVEE-QMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTDQINV 749
            ME     F EEL+  R++TW T +    +    +G + D FD+N L  P L  S      P     D+ F   YG   D  S   +D F         T P L  +  P+L                 LE  + +CK+E  Q SE+PVF+MG               SK+++GQPSKNLMAE      LNDRLSMLR+IVPKISKMDRTSILGDTIDYMKELLE+I  LQ+E++  ++   +
Sbjct:    1 MEFYDQDFLEELMTLRRETWDT-NPRTEENQFFDGGSFDCFDQNSL--PYLPNSSCGQEVPQTYNNDYPFSEIYGCLLDESSPQIMDSFYSTLDTPLNTPPFLGQEDYPILSMMEEEEQGLLGEELKNLE-LQTTCKMEPTQSSEMPVFNMGT-------GLERKNRSKKLQGQPSKNLMAERRRRKRLNDRLSMLRAIVPKISKMDRTSILGDTIDYMKELLEKINNLQQEVEVDSNMAGI 231

[Close]

BLAST of GT623650 vs. TrEMBL
Match: D7MU05_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496795 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 8.137e-24
Identity = 92/249 (36.95%), Postives = 115/249 (46.18%), Query Frame = 3

Query:   75 MELS-QLGFFEELLAPRKDTWS------TLSTGLNDLLLPNGWNLDSF-------DENLLINPSL---------------NPSFASFSTPLDHRFECSYGTDAVSYPFVDGFT--VPELDDSTPLLPQXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            MELS Q+  FEELL P K   +      + + G +    PN +N+D         DEN L+ PS                 P     S PL      +    +  YPF++     +     S PL+ Q                     S  +E   S+   F +G C             +K++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGD IDYMKELL++I KLQ+E  E
Sbjct:    1 MELSSQMNVFEELLVPTKQETTDNINNMSFNGGFDHQFFPNAYNIDYLCFNNEEEDENTLLYPSSFMDLISQPPPLLLHQPPPLQPLSPPLSS--SATAAAASFDYPFLEALQEIIDSSSSSPPLILQNGQEENFNNPTSYP-------SPLMESDQSKS--FSVGYCGGETNKKK-----NKKLEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDAIDYMKELLDKINKLQDEEQE 233

[Close]

BLAST of GT623650 vs. SwissProt
Match: BH093_ARATH (Transcription factor bHLH93 OS=Arabidopsis thaliana GN=BHLH93 PE=1 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.420e-25
Identity = 97/255 (38.04%), Postives = 115/255 (45.10%), Query Frame = 3

Query:   75 MELS-QLGFFEELLAPRKDTWSTLSTGLNDL------------LLPNGWNLDSF-------DENLLINPSL---------------NPSFASFSTPLDHRFECSYGTDAVSYPFVDGFT--VPELDDSTPLLPQXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            MELS Q+  FEELL P K    T    +N+L              PNG+N+D         DEN L+ PS                 P     S PL          D   YPF++     +     S PL+ Q                     S  +E   S+   F +G C             SK++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGD IDYMKELL++I KLQ+E  E
Sbjct:    1 MELSTQMNVFEELLVPTKQ--ETTDNNINNLSFNGGFDHHHHQFFPNGYNIDYLCFNNEEEDENTLLYPSSFMDLISQPPPLLLHQPPPLQPLSPPLSSSATAGATFD---YPFLEALQEIIDSSSSSPPLILQNGQEENFNNPMSYP-------SPLMESDQSKS--FSVGYCGGETNKKK-----SKKLEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDAIDYMKELLDKINKLQDEEQE 236

[Close]

BLAST of GT623650 vs. SwissProt
Match: BH061_ARATH (Transcription factor bHLH61 OS=Arabidopsis thaliana GN=BHLH61 PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 4.722e-21
Identity = 75/207 (36.23%), Postives = 96/207 (46.38%), Query Frame = 3

Query:  159 DLLLPNGWNLDSFDENLLINPSLNPSFASFST---------PLDHR--------FECSYGTDAVSYPFVDGFTVPELDDSTPLLPQXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            D  +PN WN D    N L+    N    S S+         PL H+        ++    + A  YPF++            +LP                      S  +EE  S I + +                 +K++EGQPSKNLMAE      LNDRLS+LRSIVPKI+KMDRTSILGD IDYMKELL++I KLQE+  E
Sbjct:   27 DQFVPNDWNFDYLCFNNLLQEDDNIDHPSSSSLMNLISQPPPLLHQPPQPSSPLYDSPPLSSAFDYPFLEDIIHSSYSPPPLILPASQE-------------NTNNYSPLMEESKSFISIGETN-----------KKRSNKKLEGQPSKNLMAERRRRKRLNDRLSLLRSIVPKITKMDRTSILGDAIDYMKELLDKINKLQEDEQE 209

[Close]

BLAST of GT623650 vs. SwissProt
Match: ICE1_ARATH (Transcription factor ICE1 OS=Arabidopsis thaliana GN=SCRM PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.681e-16
Identity = 42/63 (66.67%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  537 EGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMD 725
            +G P+KNLMAE      LNDRL MLRS+VPKISKMDR SILGD IDY+KELL+RI  L  E++
Sbjct:  302 KGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELE 364

[Close]

BLAST of GT623650 vs. SwissProt
Match: SCRM2_ARATH (Transcription factor SCREAM2 OS=Arabidopsis thaliana GN=SCRM2 PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 3.502e-16
Identity = 42/63 (66.67%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  537 EGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMD 725
            +G P+KNLMAE      LNDRL MLRS+VPKISKMDR SILGD IDY+KELL+RI  L  E++
Sbjct:  262 KGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHTELE 324

[Close]

BLAST of GT623650 vs. SwissProt
Match: AMS_ARATH (Transcription factor ABORTED MICROSPORES OS=Arabidopsis thaliana GN=AMS PE=1 SV=2)

HSP 1 Score: 73.559 bits (179), Expect = 1.377e-12
Identity = 36/70 (51.43%), Postives = 50/70 (71.43%), Query Frame = 3

Query:  528 KRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTD 737
            K  +G  +KNLMAE      LNDRL  LRS+VP+I+K+DR SILGD I+Y+KEL     +LQ+E++E ++
Sbjct:  306 KSGKGSQAKNLMAERRRRKKLNDRLYALRSLVPRITKLDRASILGDAINYVKELQNEAKELQDELEENSE 375

[Close]

BLAST of GT623650 vs. SwissProt
Match: AIB_ARATH (Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Arabidopsis thaliana GN=AIB PE=2 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 1.206e-8
Identity = 31/66 (46.97%), Postives = 43/66 (65.15%), Query Frame = 3

Query:  543 QPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTDQ 740
            +P  ++ AE      LN R   LRS+VP ISKMD+ S+LGD I Y+KEL E++ K+ E+   GTD+
Sbjct:  392 EPLNHVEAERQRREKLNQRFYALRSVVPNISKMDKASLLGDAISYIKELQEKV-KIMEDERVGTDK 456

[Close]

BLAST of GT623650 vs. SwissProt
Match: BH027_ARATH (Transcription factor bHLH27 OS=Arabidopsis thaliana GN=BHLH27 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.688e-8
Identity = 28/60 (46.67%), Postives = 43/60 (71.67%), Query Frame = 3

Query:  549 SKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            SKN+++E      LN RL  LRS+VP ISK+D+ S++ D+IDYM+EL+++   L+ E+ E
Sbjct:   53 SKNVVSERNRRQKLNQRLFALRSVVPNISKLDKASVIKDSIDYMQELIDQEKTLEAEIRE 112

[Close]

BLAST of GT623650 vs. SwissProt
Match: BH004_ARATH (Transcription factor MYC4 OS=Arabidopsis thaliana GN=BHLH4 PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 7.820e-8
Identity = 27/70 (38.57%), Postives = 43/70 (61.43%), Query Frame = 3

Query:  543 QPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTDQINVL 752
            +P  ++ AE      LN R   LR++VP +SKMD+ S+LGD I Y+ EL  ++ K + + +E   QI+V+
Sbjct:  413 EPLNHVEAERQRREKLNQRFYSLRAVVPNVSKMDKASLLGDAISYISELKSKLQKAESDKEELQKQIDVM 482

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT5G65640.2 (| Symbols: bHLH093 | beta HLH protein 93 | chr5:26237137-26238635 FORWARD LENGTH=290)

HSP 1 Score: 115.931 bits (289), Expect = 2.395e-26
Identity = 97/255 (38.04%), Postives = 115/255 (45.10%), Query Frame = 3

Query:   75 MELS-QLGFFEELLAPRKDTWSTLSTGLNDL------------LLPNGWNLDSF-------DENLLINPSL---------------NPSFASFSTPLDHRFECSYGTDAVSYPFVDGFT--VPELDDSTPLLPQXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            MELS Q+  FEELL P K    T    +N+L              PNG+N+D         DEN L+ PS                 P     S PL          D   YPF++     +     S PL+ Q                     S  +E   S+   F +G C             SK++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGD IDYMKELL++I KLQ+E  E
Sbjct:    1 MELSTQMNVFEELLVPTKQ--ETTDNNINNLSFNGGFDHHHHQFFPNGYNIDYLCFNNEEEDENTLLYPSSFMDLISQPPPLLLHQPPPLQPLSPPLSSSATAGATFD---YPFLEALQEIIDSSSSSPPLILQNGQEENFNNPMSYP-------SPLMESDQSKS--FSVGYCGGETNKKK-----SKKLEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDAIDYMKELLDKINKLQDEEQE 236

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT5G65640.1 (| Symbols: bHLH093 | beta HLH protein 93 | chr5:26237137-26238904 FORWARD LENGTH=351)

HSP 1 Score: 115.931 bits (289), Expect = 2.395e-26
Identity = 97/255 (38.04%), Postives = 115/255 (45.10%), Query Frame = 3

Query:   75 MELS-QLGFFEELLAPRKDTWSTLSTGLNDL------------LLPNGWNLDSF-------DENLLINPSL---------------NPSFASFSTPLDHRFECSYGTDAVSYPFVDGFT--VPELDDSTPLLPQXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            MELS Q+  FEELL P K    T    +N+L              PNG+N+D         DEN L+ PS                 P     S PL          D   YPF++     +     S PL+ Q                     S  +E   S+   F +G C             SK++EGQPSKNLMAE      LNDRLSMLRSIVPKISKMDRTSILGD IDYMKELL++I KLQ+E  E
Sbjct:    1 MELSTQMNVFEELLVPTKQ--ETTDNNINNLSFNGGFDHHHHQFFPNGYNIDYLCFNNEEEDENTLLYPSSFMDLISQPPPLLLHQPPPLQPLSPPLSSSATAGATFD---YPFLEALQEIIDSSSSSPPLILQNGQEENFNNPMSYP-------SPLMESDQSKS--FSVGYCGGETNKKK-----SKKLEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDAIDYMKELLDKINKLQDEEQE 236

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT5G10570.1 (| Symbols: | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr5:3341357-3342877 FORWARD LENGTH=315)

HSP 1 Score: 101.679 bits (252), Expect = 4.675e-22
Identity = 75/207 (36.23%), Postives = 96/207 (46.38%), Query Frame = 3

Query:  159 DLLLPNGWNLDSFDENLLINPSLNPSFASFST---------PLDHR--------FECSYGTDAVSYPFVDGFTVPELDDSTPLLPQXXXXXXXXXXXXXXXLEERKISCKVEEQMSEIPVFDMGLCXXXXXXXXXXXXXSKRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            D  +PN WN D    N L+    N    S S+         PL H+        ++    + A  YPF++            +LP                      S  +EE  S I + +                 +K++EGQPSKNLMAE      LNDRLS+LRSIVPKI+KMDRTSILGD IDYMKELL++I KLQE+  E
Sbjct:   27 DQFVPNDWNFDYLCFNNLLQEDDNIDHPSSSSLMNLISQPPPLLHQPPQPSSPLYDSPPLSSAFDYPFLEDIIHSSYSPPPLILPASQE-------------NTNNYSPLMEESKSFISIGETN-----------KKRSNKKLEGQPSKNLMAERRRRKRLNDRLSLLRSIVPKITKMDRTSILGDAIDYMKELLDKINKLQEDEQE 209

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT3G26744.4 (| Symbols: ICE1, ATICE1, SCRM | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr3:9832953-9834790 REVERSE LENGTH=494)

HSP 1 Score: 85.8853 bits (211), Expect = 2.655e-17
Identity = 42/63 (66.67%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  537 EGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMD 725
            +G P+KNLMAE      LNDRL MLRS+VPKISKMDR SILGD IDY+KELL+RI  L  E++
Sbjct:  302 KGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELE 364

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT3G26744.2 (| Symbols: ICE1, ATICE1, SCRM | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr3:9832953-9834790 REVERSE LENGTH=494)

HSP 1 Score: 85.8853 bits (211), Expect = 2.655e-17
Identity = 42/63 (66.67%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  537 EGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMD 725
            +G P+KNLMAE      LNDRL MLRS+VPKISKMDR SILGD IDY+KELL+RI  L  E++
Sbjct:  302 KGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELE 364

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT3G26744.1 (| Symbols: ICE1, ATICE1, SCRM | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr3:9832953-9834790 REVERSE LENGTH=494)

HSP 1 Score: 85.8853 bits (211), Expect = 2.655e-17
Identity = 42/63 (66.67%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  537 EGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMD 725
            +G P+KNLMAE      LNDRL MLRS+VPKISKMDR SILGD IDY+KELL+RI  L  E++
Sbjct:  302 KGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELE 364

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT1G12860.1 (| Symbols: SCRM2, ICE2 | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr1:4384758-4386392 FORWARD LENGTH=450)

HSP 1 Score: 85.5001 bits (210), Expect = 3.467e-17
Identity = 42/63 (66.67%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  537 EGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMD 725
            +G P+KNLMAE      LNDRL MLRS+VPKISKMDR SILGD IDY+KELL+RI  L  E++
Sbjct:  262 KGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHTELE 324

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT2G16910.1 (| Symbols: AMS | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr2:7331721-7334077 FORWARD LENGTH=571)

HSP 1 Score: 73.559 bits (179), Expect = 1.363e-13
Identity = 36/70 (51.43%), Postives = 50/70 (71.43%), Query Frame = 3

Query:  528 KRVEGQPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTD 737
            K  +G  +KNLMAE      LNDRL  LRS+VP+I+K+DR SILGD I+Y+KEL     +LQ+E++E ++
Sbjct:  306 KSGKGSQAKNLMAERRRRKKLNDRLYALRSLVPRITKLDRASILGDAINYVKELQNEAKELQDELEENSE 375

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT2G46510.1 (| Symbols: ATAIB, AIB | ABA-inducible BHLH-type transcription factor | chr2:19091187-19092887 REVERSE LENGTH=566)

HSP 1 Score: 60.4622 bits (145), Expect = 1.194e-9
Identity = 31/66 (46.97%), Postives = 43/66 (65.15%), Query Frame = 3

Query:  543 QPSKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDEGTDQ 740
            +P  ++ AE      LN R   LRS+VP ISKMD+ S+LGD I Y+KEL E++ K+ E+   GTD+
Sbjct:  392 EPLNHVEAERQRREKLNQRFYALRSVVPNISKMDKASLLGDAISYIKELQEKV-KIMEDERVGTDK 456

[Close]

BLAST of GT623650 vs. TAIR peptide
Match: AT4G29930.4 (| Symbols: | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr4:14644108-14645168 FORWARD LENGTH=184)

HSP 1 Score: 59.3066 bits (142), Expect = 2.661e-9
Identity = 28/60 (46.67%), Postives = 43/60 (71.67%), Query Frame = 3

Query:  549 SKNLMAEXXXXXXLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERIGKLQEEMDE 728
            SKN+++E      LN RL  LRS+VP ISK+D+ S++ D+IDYM+EL+++   L+ E+ E
Sbjct:   53 SKNVVSERNRRQKLNQRLFALRSVVPNISKLDKASVIKDSIDYMQELIDQEKTLEAEIRE 112

The following BLAST results are available for this feature:

BLAST of GT623650 vs. TrEMBL
BLAST of GT623650 vs. SwissProt
BLAST of GT623650 vs. TAIR peptide

BLAST of GT623650 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A9YWR2_MEDTR	1.967e-86	73.82	BHLH transcription factor OS=Medicago truncatula P...	[more]
C6TNX2_SOYBN	3.848e-74	65.57	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
C6TA88_SOYBN	2.581e-70	63.93	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q2HRF0_MEDTR	5.791e-46	58.82	Helix-loop-helix DNA-binding OS=Medicago truncatul...	[more]
B9SSC2_RICCO	3.513e-43	49.62	DNA binding protein, putative OS=Ricinus communis ...	[more]
A5BDS2_VITVI	2.784e-40	44.90	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9SMX0_RICCO	1.867e-36	42.02	DNA binding protein, putative OS=Ricinus communis ...	[more]
A9P9D2_POPTR	1.871e-28	36.33	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C6TH74_SOYBN	9.288e-28	40.08	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7MU05_ARALY	8.137e-24	36.95	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of GT623650 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
BH093_ARATH	2.420e-25	38.04	Transcription factor bHLH93 OS=Arabidopsis thalian...	[more]
BH061_ARATH	4.722e-21	36.23	Transcription factor bHLH61 OS=Arabidopsis thalian...	[more]
ICE1_ARATH	2.681e-16	66.67	Transcription factor ICE1 OS=Arabidopsis thaliana ...	[more]
SCRM2_ARATH	3.502e-16	66.67	Transcription factor SCREAM2 OS=Arabidopsis thalia...	[more]
AMS_ARATH	1.377e-12	51.43	Transcription factor ABORTED MICROSPORES OS=Arabid...	[more]
AIB_ARATH	1.206e-8	46.97	Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Ar...	[more]
BH027_ARATH	2.688e-8	46.67	Transcription factor bHLH27 OS=Arabidopsis thalian...	[more]
BH004_ARATH	7.820e-8	38.57	Transcription factor MYC4 OS=Arabidopsis thaliana ...	[more]

BLAST of GT623650 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G65640.2	2.395e-26	38.04	\| Symbols: bHLH093 \| beta HLH protein 93 \| chr5:26...	[more]
AT5G65640.1	2.395e-26	38.04	\| Symbols: bHLH093 \| beta HLH protein 93 \| chr5:26...	[more]
AT5G10570.1	4.675e-22	36.23	\| Symbols: \| basic helix-loop-helix (bHLH) DNA-bi...	[more]
AT3G26744.4	2.655e-17	66.67	\| Symbols: ICE1, ATICE1, SCRM \| basic helix-loop-h...	[more]
AT3G26744.2	2.655e-17	66.67	\| Symbols: ICE1, ATICE1, SCRM \| basic helix-loop-h...	[more]
AT3G26744.1	2.655e-17	66.67	\| Symbols: ICE1, ATICE1, SCRM \| basic helix-loop-h...	[more]
AT1G12860.1	3.467e-17	66.67	\| Symbols: SCRM2, ICE2 \| basic helix-loop-helix (b...	[more]
AT2G16910.1	1.363e-13	51.43	\| Symbols: AMS \| basic helix-loop-helix (bHLH) DNA...	[more]
AT2G46510.1	1.194e-9	46.97	\| Symbols: ATAIB, AIB \| ABA-inducible BHLH-type tr...	[more]
AT4G29930.4	2.661e-9	46.67	\| Symbols: \| basic helix-loop-helix (bHLH) DNA-bi...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 7..23 score
None	No IPR available	PANTHER	PTHR12565	STEROL REGULATORY ELEMENT-BINDING PROTEIN	coord: 189..231 score: 0.0
None	No IPR available	PANTHER	PTHR12565:SF7	CENTROMERE-BINDING PROTEIN 1, CBP-1	coord: 189..231 score: 0.0
None	No IPR available	SEG	seg	seg	coord: 3..15 score: NAcoord: 122..136 score: NAcoord: 162..174 score: NAcoord: 190..195 score
None	No IPR available	SEG	seg	seg	coord: 21..41 score
None	No IPR available	SEG	seg	seg	coord: 31..46 score
IPR001092	Helix-loop-helix DNA-binding domain	PFAM	PF00010	HLH	coord: 186..229 score: 8.9
IPR001092	Helix-loop-helix DNA-binding domain	SMART	SM00353	no description	coord: 186..235 score: 1.2
IPR001092	Helix-loop-helix DNA-binding domain	PROFILE	PS50888	HLH	coord: 174..230 score: 12
IPR011598	Helix-loop-helix DNA-binding	GENE3D	4.10.280.10	no description	coord: 187..249 score: 3.7
IPR011598	Helix-loop-helix DNA-binding	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 170..247 score: 3.1

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT623650 ID=GT623650; Name=GT623650; organism=Lens culinaris; type=EST; length=761bp
CTTATTTTCTTCTTCTTCATCTTCTGTTTAGTTTACTCATAGCTCTCAAA
AGAGAGAAAAATAACAGAGAAAACATGGAGCTTTCTCAACTTGGTTTCTT
TGAGGAACTACTAGCTCCAAGAAAAGACACATGGAGTACTTTATCCACCG
GTTTAAACGACCTACTACTTCCTAATGGTTGGAACTTGGACTCATTTGAT
GAAAATCTACTCATCAACCCATCTCTAAACCCTTCATTTGCTTCGTTTTC
AACACCATTAGACCATAGATTTGAATGCTCTTATGGAACTGACGCTGTTT
CATACCCTTTTGTTGATGGGTTCACTGTTCCAGAGCTTGATGATTCAACT
CCACTTTTACCTCAAGAGGGTGTTGAAGAGTTCGGTTTTGTTGGAAGTGA
GGAGAAGGGTTTGGAAGAGAGGAAAATTAGCTGTAAAGTTGAGGAACAAA
TGAGTGAGATTCCTGTTTTTGACATGGGTTTGTGTGGTGGTGGTGGTGGT
GGTGGTGGTCAGAAGAAAGGTAAATCCAAAAGGGTTGAAGGACAACCTTC
GAAGAATCTCATGGCTGAAAGAAGGAGAAGAAAGAGGTTGAACGATAGAC
TTTCCATGCTTAGGTCAATTGTCCCTAAGATCAGTAAGATGGACAGAACA
TCGATTCTCGGAGATACAATTGATTACATGAAAGAGCTTCTTGAAAGGAT
TGGTAAGTTGCAAGAAGAAATGGATGAAGGAACAGATCAGATAAATGTTT
TGGGAATTTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0030528	obsolete transcription regulator activity

Vocabulary: **Biological Process**
Term	Definition
GO:0045449	regulation of transcription

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
IPR001092	Helix-loop-helix DNA-binding domain
IPR011598	bHLH_dom

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