GT627144

EST Overview


Name	GT627144
Unique Name	GT627144
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	753

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT627144 vs. TrEMBL
Match: C6TCL7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 6.663e-79
Identity = 151/189 (79.89%), Postives = 161/189 (85.19%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            DH VYEQ YL HVLQTILKVV DYAKDRPIIFSTFQP+AA+L+RKLQ  YPVFFLTNGGCE YED+RRNSLEEA+KLCLENGL+GIVSEIKGIFRNPGAV+KIKES LSLL+YGKLNNVPEAVYMQHLMGIDGVIVD VQEITEAVADM+KP+K                 SKPQFSQ ELSFL KLIP
Sbjct:  188 DHFVYEQDYLVHVLQTILKVVFDYAKDRPIIFSTFQPEAAMLIRKLQTNYPVFFLTNGGCEIYEDVRRNSLEEAMKLCLENGLEGIVSEIKGIFRNPGAVTKIKESKLSLLSYGKLNNVPEAVYMQHLMGIDGVIVDLVQEITEAVADMIKPTKGGEGEGLVEGCGKVQGNSKPQFSQQELSFLLKLIP 376

[Close]

BLAST of GT627144 vs. TrEMBL
Match: B9N0X1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_783367 PE=4 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 2.998e-71
Identity = 135/189 (71.43%), Postives = 156/189 (82.54%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            DHIVY+Q YL  VLQ IL+VV D+ KDRP+IFS+FQPDAA+LVRKLQ T+PV+FLTNGG E + D RRNSLEEA+K+CLE GL GIVSE+KG+FRNPGAV+KIK++ LSLLTYGKLNNV EAVYMQHLMGIDGVIVD VQEITEAV+D++KPSK+                SKPQFSQ+ELSFL KLIP
Sbjct:  189 DHIVYQQDYLIRVLQAILQVVFDHGKDRPVIFSSFQPDAALLVRKLQSTFPVYFLTNGGTEIFYDARRNSLEEAIKVCLEGGLQGIVSEVKGVFRNPGAVNKIKDAKLSLLTYGKLNNVSEAVYMQHLMGIDGVIVDLVQEITEAVSDLIKPSKMGEAESLAEGDGEMEVKSKPQFSQMELSFLLKLIP 377

[Close]

BLAST of GT627144 vs. TrEMBL
Match: A5AMY0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038621 PE=4 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 2.538e-70
Identity = 137/189 (72.49%), Postives = 154/189 (81.48%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            D+IVYEQ YLTHVLQ +++VV  YAKDRPIIFSTFQPDAA LVRKLQ +YPVFFLTNGG E Y D+RRNSLEEA+KLCLE GL GIVS++K +FRNP AV+KIKES LSLLTYG+LNNV EAVYMQHLMG++GVIVD V+EITEAV+D+MKPSK                 SKPQFSQ ELSFL KLIP
Sbjct:  189 DNIVYEQEYLTHVLQAMVEVVFQYAKDRPIIFSTFQPDAAQLVRKLQSSYPVFFLTNGGTEVYYDVRRNSLEEAVKLCLEGGLQGIVSQVKAVFRNPAAVTKIKESKLSLLTYGQLNNVAEAVYMQHLMGVEGVIVDLVKEITEAVSDLMKPSKEGDEESLPEGVGQMQVKSKPQFSQRELSFLLKLIP 377

[Close]

BLAST of GT627144 vs. TrEMBL
Match: D7SVL1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_68.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033033001 PE=4 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 2.806e-69
Identity = 137/189 (72.49%), Postives = 154/189 (81.48%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            D+IVYEQ YLTHVLQ +++VV  YAKDRPIIFSTFQPDAA LVRKLQ +YPVFFLTNGG E Y D+RRNSLEEA+KLCLE GL GIVS++K +FRNP AV+KIKES LSLLTYG+LNNV EAVYMQHLMG++GVIVD V+EITEAV+D+MKPSK                 SKPQFSQ ELSFL KLIP
Sbjct:  189 DNIVYEQEYLTHVLQAMVEVVFQYAKDRPIIFSTFQPDAAQLVRKLQSSYPVFFLTNGGTEVYYDVRRNSLEEAVKLCLEGGLQGIVSQVKAVFRNPAAVTKIKESKLSLLTYGQLNNVAEAVYMQHLMGVEGVIVDLVKEITEAVSDLMKPSK----------EGQMQVKSKPQFSQRELSFLLKLIP 367

[Close]

BLAST of GT627144 vs. TrEMBL
Match: B9GF71_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_174634 PE=4 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 2.375e-68
Identity = 130/189 (68.78%), Postives = 155/189 (82.01%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            +HIVY+Q YL  VLQ IL+VV ++ KDRPIIFS+FQPDAA+LVRKLQ TYPV+FLT+GG E + D+RRNSLEEA+K+CLE GL GIVSE+K +FRNPGAVSKIK++ LSL+TYGKLNNV EAVY+QHLMGIDGVIVD VQEITEAV+D+++PSK+                SKP+FSQ ELSFL KLIP
Sbjct:  179 NHIVYQQDYLIRVLQAILQVVFEHGKDRPIIFSSFQPDAALLVRKLQSTYPVYFLTDGGVEIFYDVRRNSLEEAIKVCLEGGLQGIVSEVKAVFRNPGAVSKIKDAKLSLITYGKLNNVSEAVYIQHLMGIDGVIVDLVQEITEAVSDLIRPSKVEEAESLAEGHDEMEEKSKPKFSQRELSFLLKLIP 367

[Close]

BLAST of GT627144 vs. TrEMBL
Match: B9RH28_RICCO (Glycerophosphodiester phosphodiesterase, putative OS=Ricinus communis GN=RCOM_1446530 PE=4 SV=1)

HSP 1 Score: 261.536 bits (667), Expect = 5.292e-68
Identity = 135/190 (71.05%), Postives = 154/190 (81.05%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKI-XXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            DHIVY+Q YL +VLQ IL+VV +Y  DRPIIFS+FQPDAA+LVR+LQ TYPV+FLTNGG E + D RRNSLEEA+K+CLE GL GIVSE+KGIFRNPGAV+KIKE+ LSLLTYGKLNNV EAVYMQHLMGIDGVIVD V+EITEAV+DM+KP+K+                 S PQFSQ ELSFL KLIP
Sbjct:  189 DHIVYQQDYLNYVLQAILQVVFEYGLDRPIIFSSFQPDAALLVRQLQCTYPVYFLTNGGTEIFYDTRRNSLEEAIKVCLEGGLHGIVSEVKGIFRNPGAVAKIKEAKLSLLTYGKLNNVSEAVYMQHLMGIDGVIVDLVKEITEAVSDMIKPAKVDDEESNLAEKDSEMEMKSIPQFSQKELSFLLKLIP 378

[Close]

BLAST of GT627144 vs. TrEMBL
Match: B9GMQ2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_548064 PE=4 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 8.183e-61
Identity = 116/188 (61.70%), Postives = 147/188 (78.19%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLI 564
            D+I+Y++  L H+LQ IL+VV ++AK+RP++FS+FQPDAA+L+RKLQ TYPVFFLTNGG E Y D+RRNSL+EA+K+C E GL GIVSE+K +FRNPGAV++IKES LSL+TYG+LNNVPE V+MQHLMGI+GVIVD V EITEAV+  +  +K                 +KP+FS  ELSFL KLI
Sbjct:  195 DNIIYKEEELKHILQVILQVVFEHAKERPVMFSSFQPDAALLMRKLQSTYPVFFLTNGGSEIYTDVRRNSLDEAIKVCTEGGLQGIVSEVKAVFRNPGAVTRIKESKLSLITYGQLNNVPEVVHMQHLMGIEGVIVDLVHEITEAVSHFVDSAKHGEESGLFAEEGKIQVKTKPRFSSEELSFLMKLI 382

[Close]

BLAST of GT627144 vs. TrEMBL
Match: Q9SGA2_ARATH (F1C9.18 protein OS=Arabidopsis thaliana GN=At3g02040 PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 2.381e-60
Identity = 123/189 (65.08%), Postives = 140/189 (74.07%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            D++ Y   +L+ +L  IL+VV D   DR IIFS+F PDAA+LVRKLQ TYPVFFLTNGG E Y D RRNSLEEA+K+CLE GL GIVSE+KG+FRNP  V+KIKES LSL+TYGKLNNV EAVYMQHLMGI+GVIVD V+EITEAV +MMKPS                   KP FS  ELSFL KLIP
Sbjct:  178 DNVFYSSDHLSRLLLPILQVVSDIGNDRTIIFSSFHPDAALLVRKLQTTYPVFFLTNGGTEMYHDTRRNSLEEAIKVCLEGGLQGIVSEVKGVFRNPALVNKIKESKLSLMTYGKLNNVAEAVYMQHLMGIEGVIVDHVEEITEAVREMMKPSN----------RDADGTKPKPNFSDRELSFLLKLIP 356

[Close]

BLAST of GT627144 vs. TrEMBL
Match: Q84WI7_ARATH (Expressed protein OS=Arabidopsis thaliana GN=At3g02040 PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 2.381e-60
Identity = 123/189 (65.08%), Postives = 140/189 (74.07%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            D++ Y   +L+ +L  IL+VV D   DR IIFS+F PDAA+LVRKLQ TYPVFFLTNGG E Y D RRNSLEEA+K+CLE GL GIVSE+KG+FRNP  V+KIKES LSL+TYGKLNNV EAVYMQHLMGI+GVIVD V+EITEAV +MMKPS                   KP FS  ELSFL KLIP
Sbjct:  178 DNVFYSSDHLSRLLLPILQVVSDIGNDRTIIFSSFHPDAALLVRKLQTTYPVFFLTNGGTEMYHDTRRNSLEEAIKVCLEGGLQGIVSEVKGVFRNPALVNKIKESKLSLMTYGKLNNVAEAVYMQHLMGIEGVIVDHVEEITEAVREMMKPSN----------RDADGTKPKPNFSDRELSFLLKLIP 356

[Close]

BLAST of GT627144 vs. TrEMBL
Match: Q2V8D7_ORYSJ (Glycerophosphoryl diester phosphodisterase (Fragment) OS=Oryza sativa subsp. japonica GN=GDPD PE=2 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 3.110e-60
Identity = 120/189 (63.49%), Postives = 147/189 (77.78%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            D++ Y++  LT +LQ ILKVV +YAKDRPIIFS+FQPDAA ++RKLQ TYPV+FLTNGG E Y D+RRNSLEEA+KLCL +G+ GIVSE +GIFR+P AV KIKE+NLSLLTYG LNNVPEAVYMQHLMG++GVIVD VQEITEAV++++   +                 + P FSQ E+SFL +LIP
Sbjct:  134 DNLEYQEEELTCILQAILKVVFEYAKDRPIIFSSFQPDAAQVMRKLQSTYPVYFLTNGGTEIYADVRRNSLEEAIKLCLASGMQGIVSEARGIFRHPAAVPKIKEANLSLLTYGTLNNVPEAVYMQHLMGVNGVIVDLVQEITEAVSELITVPE-PDLNADNLSNGAAKDAATPHFSQCEISFLLRLIP 321

[Close]

BLAST of GT627144 vs. SwissProt
Match: GDE1_YEAST (Glycerophosphodiester phosphodiesterase GDE1 OS=Saccharomyces cerevisiae GN=GDE1 PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.146e-20
Identity = 56/133 (42.11%), Postives = 74/133 (55.64%), Query Frame = 1

Query:   28 LTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEI 426
            + H + T+LKVV D A  R IIFS+F PD  I++   Q   P+ FLT GG E   DLR +SL+  ++   +  L GIVS    I + P  V  +K + L  +TYG  NN PE   +Q   G+D VIVD V  I
Sbjct: 1081 MNHWVDTVLKVVFDNANGRDIIFSSFHPDICIMLSLKQPVIPILFLTEGGSEQMADLRASSLQNGIRFAKKWNLLGIVSAAAPILKAPRLVQVVKSNGLVCVTYGVDNNDPENASIQIEAGVDAVIVDSVLAI 1213

[Close]

BLAST of GT627144 vs. SwissProt
Match: GDE1_ASHGO (Glycerophosphodiester phosphodiesterase GDE1 OS=Ashbya gossypii GN=GDE1 PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.073e-17
Identity = 50/137 (36.50%), Postives = 75/137 (54.74%), Query Frame = 1

Query:   28 LTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAV 438
            + H + T+L+VV D  + R +IFS+FQPD  +++   Q ++P+ FLT GG     D+R  SL+ A++      L GIVS    I   P     +K S L  +TYG  NN PE   ++   G+D VIVD V  + + +
Sbjct: 1175 MNHWIDTVLEVVYDNVEGRDVIFSSFQPDVCLMLSLKQPSFPILFLTEGGTAKRCDIRAASLQNAIRFAHRWNLLGIVSAAAPIVIAPRLAQIVKSSGLVCVTYGVENNDPEIARVEMDAGVDAVIVDSVLAVRKGL 1311

[Close]

BLAST of GT627144 vs. SwissProt
Match: GDE1_SCHPO (Glycerophosphodiester phosphodiesterase gde1 OS=Schizosaccharomyces pombe GN=gde1 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.401e-17
Identity = 54/133 (40.60%), Postives = 72/133 (54.14%), Query Frame = 1

Query:   40 LQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAV 438
            + TIL ++  Y   R  IFS+F PD  IL+  L+ T PV FLT GG  +  D+R  SL +ALK   +    GIVS  + +   P  +  +K+  LS  TYG LNN  + V  Q   G+D VIVD V  I  A+
Sbjct:  935 VDTILSIIKKYGGKRKYIFSSFNPDICILL-SLKSTNPVLFLTEGGTAYRTDVRAASLRQALKFASQWSFLGIVSACEPLIMCPRLIKAVKQLGLSCYTYGVLNNDVDNVRRQVRFGVDAVIVDNVLAIRRAL 1066

[Close]

BLAST of GT627144 vs. SwissProt
Match: GPCP1_RAT (Putative glycerophosphocholine phosphodiesterase GPCPD1 OS=Rattus norvegicus GN=Gpcpd1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.149e-11
Identity = 41/131 (31.30%), Postives = 65/131 (49.62%), Query Frame = 1

Query:   40 LQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYE---DLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQE 423
            L  ILK V++ +  R I+FS+F  D   +VR+ Q  YP+ FLT G  + Y    DLR  +   A+       + GI +  + + RNP  V + K+  L +  +G   N PE        G++G+I D + +
Sbjct:  483 LDIILKTVLENSGKRRIVFSSFDADICTMVRQKQNKYPILFLTQGKSDIYPELMDLRSRTTPIAMSFAQFENILGINAHTEDLLRNPSYVQEAKDKGLVIFCWGDDTNDPENRRKLKEFGVNGLIYDRIYD 613

[Close]

BLAST of GT627144 vs. SwissProt
Match: GPCP1_HUMAN (Putative glycerophosphocholine phosphodiesterase GPCPD1 OS=Homo sapiens GN=GPCPD1 PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 1.149e-11
Identity = 42/131 (32.06%), Postives = 64/131 (48.85%), Query Frame = 1

Query:   40 LQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYE---DLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQE 423
            L  ILK V++ +  R I+FS+F  D   +VR+ Q  YP+ FLT G  E Y    DLR  +   A+       L GI    + + RNP  + + K   L +  +G   N PE       +G++G+I D + +
Sbjct:  483 LDIILKTVLENSGKRRIVFSSFDADICTMVRQKQNKYPILFLTQGKSEIYPELMDLRSRTTPIAMSFAQFENLLGINVHTEDLLRNPSYIQEAKAKGLVIFCWGDDTNDPENRRKLKELGVNGLIYDRIYD 613

[Close]

BLAST of GT627144 vs. SwissProt
Match: GPCP1_MOUSE (Putative glycerophosphocholine phosphodiesterase GPCPD1 OS=Mus musculus GN=Gpcpd1 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.560e-11
Identity = 41/131 (31.30%), Postives = 64/131 (48.85%), Query Frame = 1

Query:   40 LQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYE---DLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQE 423
            L  ILK V++ +  R I+FS+F  D   +VR+ Q  YP+ FLT G  + Y    DLR  +   A+       + GI +  + + RNP  V + K   L +  +G   N PE        G++G+I D + +
Sbjct:  486 LDIILKTVLENSGKRRIVFSSFDADICTMVRQKQNKYPILFLTQGKSDIYPELMDLRSRTTPIAMSFAQFENILGINAHTEDLLRNPSYVQEAKAKGLVIFCWGDDTNDPENRRKLKEFGVNGLIYDRIYD 616

[Close]

BLAST of GT627144 vs. SwissProt
Match: GPC1A_CAEEL (Putative glycerophosphocholine phosphodiesterase GPCPD1 homolog T05H10.7 OS=Caenorhabditis elegans GN=T05H10.7 PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 7.707e-8
Identity = 39/132 (29.55%), Postives = 64/132 (48.48%), Query Frame = 1

Query:   40 LQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFY---EDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGK-LNNVPEAVYMQHLMGIDGVIVDFVQE 423
            +  IL  V+ +A +R I+FS+F PD   +V   Q  YPV FL  G  + Y   +D R ++   A+       L G+    + + ++P  V K  E  +    +G+ L+      Y +  +G+DGVI D + E
Sbjct:  575 VDVILADVMKHAGNRRIMFSSFDPDICSMVATKQNKYPVLFLCVGETQRYTPFQDQRTSTSMTAVNFAAGADLLGVNFNSEDLLKDPMPVKKANEFGMVTFVWGEDLDKKENINYFKKELGVDGVIYDRIGE 706

[Close]

BLAST of GT627144 vs. TAIR peptide
Match: AT3G02040.1 (| Symbols: SRG3 | senescence-related gene 3 | chr3:348505-349909 REVERSE LENGTH=361)

HSP 1 Score: 236.113 bits (601), Expect = 1.568e-62
Identity = 123/189 (65.08%), Postives = 140/189 (74.07%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLIP 567
            D++ Y   +L+ +L  IL+VV D   DR IIFS+F PDAA+LVRKLQ TYPVFFLTNGG E Y D RRNSLEEA+K+CLE GL GIVSE+KG+FRNP  V+KIKES LSL+TYGKLNNV EAVYMQHLMGI+GVIVD V+EITEAV +MMKPS                   KP FS  ELSFL KLIP
Sbjct:  178 DNVFYSSDHLSRLLLPILQVVSDIGNDRTIIFSSFHPDAALLVRKLQTTYPVFFLTNGGTEMYHDTRRNSLEEAIKVCLEGGLQGIVSEVKGVFRNPALVNKIKESKLSLMTYGKLNNVAEAVYMQHLMGIEGVIVDHVEEITEAVREMMKPSN----------RDADGTKPKPNFSDRELSFLLKLIP 356

[Close]

BLAST of GT627144 vs. TAIR peptide
Match: AT5G41080.2 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr5:16441989-16443835 FORWARD LENGTH=358)

HSP 1 Score: 233.802 bits (595), Expect = 7.781e-62
Identity = 117/188 (62.23%), Postives = 145/188 (77.13%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLI 564
            D  VYE+ +L H+L+++L+VV +YAKDRP+IFS+FQPDAA LVR+LQ TYPVFFLT+ G E + D RRNSLEEA+++CLE GL GIVSE+KG+FRNP A+SKIKESNLSLLTYGKLNNV EAVYMQ++MGIDGVIVDFV+EI E+   MM                     ++P+FSQ E+SFL KL+
Sbjct:  165 DQTVYEREFLVHILRSVLQVVSNYAKDRPVIFSSFQPDAAKLVRELQSTYPVFFLTDAGNEIHNDERRNSLEEAIQVCLEGGLQGIVSEVKGVFRNPAAISKIKESNLSLLTYGKLNNVGEAVYMQYVMGIDGVIVDFVEEIIESTTRMMIRPPPSSSPLPSPSKDDDVAITRPEFSQKEISFLLKLL 352

[Close]

BLAST of GT627144 vs. TAIR peptide
Match: AT5G41080.1 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr5:16441858-16443835 FORWARD LENGTH=374)

HSP 1 Score: 233.802 bits (595), Expect = 7.781e-62
Identity = 117/188 (62.23%), Postives = 145/188 (77.13%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADMMKPSKIXXXXXXXXXXXXXXXXSKPQFSQLELSFLYKLI 564
            D  VYE+ +L H+L+++L+VV +YAKDRP+IFS+FQPDAA LVR+LQ TYPVFFLT+ G E + D RRNSLEEA+++CLE GL GIVSE+KG+FRNP A+SKIKESNLSLLTYGKLNNV EAVYMQ++MGIDGVIVDFV+EI E+   MM                     ++P+FSQ E+SFL KL+
Sbjct:  181 DQTVYEREFLVHILRSVLQVVSNYAKDRPVIFSSFQPDAAKLVRELQSTYPVFFLTDAGNEIHNDERRNSLEEAIQVCLEGGLQGIVSEVKGVFRNPAAISKIKESNLSLLTYGKLNNVGEAVYMQYVMGIDGVIVDFVEEIIESTTRMMIRPPPSSSPLPSPSKDDDVAITRPEFSQKEISFLLKLL 368

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BLAST of GT627144 vs. TAIR peptide
Match: AT5G43300.1 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr5:17376946-17379070 REVERSE LENGTH=370)

HSP 1 Score: 193.741 bits (491), Expect = 8.923e-50
Identity = 88/149 (59.06%), Postives = 123/149 (82.55%), Query Frame = 1

Query:    1 DHIVYEQAYLTHVLQTILKVVVDYAKDRPIIFSTFQPDAAILVRKLQKTYPVFFLTNGGCEFYEDLRRNSLEEALKLCLENGLDGIVSEIKGIFRNPGAVSKIKESNLSLLTYGKLNNVPEAVYMQHLMGIDGVIVDFVQEITEAVADM 447
            D+ VY +  L   L  IL VV +++K+RPIIFS+F PDAA L+R +Q+ YPVFFLTNGGCE Y+D+RRNSL+EA+KLC E+GL G+VSE+K I R P A++++K+S LSLL+YG+LNNV E +Y+Q+LMG++GVIVD V++I+EA+A++
Sbjct:  177 DNTVYGEGELRQTLDNILTVVNEHSKNRPIIFSSFHPDAARLIRNMQRCYPVFFLTNGGCEIYKDVRRNSLDEAIKLCKESGLQGLVSEVKAILRTPNAITRVKDSKLSLLSYGQLNNVVEVIYLQYLMGVEGVIVDMVKDISEAIANI 325

The following BLAST results are available for this feature:

BLAST of GT627144 vs. TrEMBL
BLAST of GT627144 vs. SwissProt
BLAST of GT627144 vs. TAIR peptide

BLAST of GT627144 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TCL7_SOYBN	6.663e-79	79.89	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9N0X1_POPTR	2.998e-71	71.43	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5AMY0_VITVI	2.538e-70	72.49	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7SVL1_VITVI	2.806e-69	72.49	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GF71_POPTR	2.375e-68	68.78	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9RH28_RICCO	5.292e-68	71.05	Glycerophosphodiester phosphodiesterase, putative ...	[more]
B9GMQ2_POPTR	8.183e-61	61.70	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SGA2_ARATH	2.381e-60	65.08	F1C9.18 protein OS=Arabidopsis thaliana GN=At3g020...	[more]
Q84WI7_ARATH	2.381e-60	65.08	Expressed protein OS=Arabidopsis thaliana GN=At3g0...	[more]
Q2V8D7_ORYSJ	3.110e-60	63.49	Glycerophosphoryl diester phosphodisterase (Fragme...	[more]

BLAST of GT627144 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
GDE1_YEAST	5.146e-20	42.11	Glycerophosphodiester phosphodiesterase GDE1 OS=Sa...	[more]
GDE1_ASHGO	1.073e-17	36.50	Glycerophosphodiester phosphodiesterase GDE1 OS=As...	[more]
GDE1_SCHPO	1.401e-17	40.60	Glycerophosphodiester phosphodiesterase gde1 OS=Sc...	[more]
GPCP1_RAT	1.149e-11	31.30	Putative glycerophosphocholine phosphodiesterase G...	[more]
GPCP1_HUMAN	1.149e-11	32.06	Putative glycerophosphocholine phosphodiesterase G...	[more]
GPCP1_MOUSE	2.560e-11	31.30	Putative glycerophosphocholine phosphodiesterase G...	[more]
GPC1A_CAEEL	7.707e-8	29.55	Putative glycerophosphocholine phosphodiesterase G...	[more]

BLAST of GT627144 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT3G02040.1	1.568e-62	65.08	\| Symbols: SRG3 \| senescence-related gene 3 \| chr3...	[more]
AT5G41080.2	7.781e-62	62.23	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]
AT5G41080.1	7.781e-62	62.23	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]
AT5G43300.1	8.923e-50	59.06	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004129	Glycerophosphoryl diester phosphodiesterase	PFAM	PF03009	GDPD	coord: 11..140 score: 4.2
IPR017946	PLC-like phosphodiesterase, TIM beta/alpha-barrel domain	GENE3D	3.20.20.190	no description	coord: 11..143 score: 2.2
IPR017946	PLC-like phosphodiesterase, TIM beta/alpha-barrel domain	SUPERFAMILY	51695	PLC-like phosphodiesterases	coord: 2..147 score: 1.4
None	No IPR available	PANTHER	PTHR22958	RELATED TO MULTIFUNCTIONAL CYCLIN-DEPENDENT KINASE-RELATED	coord: 1..153 score: 2.3
None	No IPR available	SEG	seg	seg	coord: 156..171 score
None	No IPR available	SEG	seg	seg	coord: 34..56 score
None	No IPR available	SEG	seg	seg	coord: 5..19 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627144 ID=GT627144; Name=GT627144; organism=Lens culinaris; type=EST; length=753bp
GACCATATTGTTTATGAACAAGCTTATCTTACTCATGTCCTTCAAACCAT
TTTAAAGGTTGTTGTTGATTATGCCAAGGATAGACCTATTATATTCTCTA
CTTTTCAACCTGATGCTGCTATACTTGTTAGGAAATTGCAAAAAACCTAC
CCTGTTTTCTTTTTAACTAATGGAGGGTGTGAATTCTATGAAGATTTGAG
AAGAAACTCTTTGGAAGAAGCTTTAAAGCTCTGTTTGGAAAATGGATTGG
ATGGAATTGTCTCTGAGATTAAAGGGATATTTAGAAATCCTGGAGCAGTA
AGCAAGATCAAGGAGTCCAATCTTTCTCTTTTAACATATGGAAAATTGAA
TAATGTCCCAGAAGCGGTTTACATGCAGCATCTAATGGGAATTGATGGGG
TAATAGTAGATTTTGTGCAAGAGATAACAGAAGCAGTTGCAGATATGATG
AAACCATCAAAAATTGGTGAGGAAGAAGGATTGAGTGAAGGACTTGAGAA
GGTGGAAGGGAAGTCAAAGCCTCAATTTTCACAGCTTGAGCTGTCTTTTC
TTTACAAGCTCATTCCACAATTACTTTTGTTATAACAACAAAGCGATATC
TTTTTTTCATATTAAACTCGATTGGTAGAAAATGGGGACAGATTTATGAA
TGACTTAACCCTTTGTACATATACGTTCTCTACGTTTCACTTGAGGATGA
AAGATATTGTGGTTCTGTATTAATACTTAGTTTGGAGGTTCTCCATTGAA
ATT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006071	glycerol metabolic process
GO:0006629	lipid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008889	glycerophosphodiester phosphodiesterase activity
GO:0008081	phosphoric diester hydrolase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR004129	GlyceroP-diester-Pdiesterase
IPR017946	PLC-like_Pdiesterase_TIM-brl

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